1.
Molecular Basis Of Antibiotic Resistanc In E. Coli Isolates From Poultry Drinking Water
by Hira Naseer | Ms. Sehrish Firyal | Prof. Dr. Masroor Ellahi Babar.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2012Dissertation note: Back Ground:
E. coli is a single-celled organism belonging to the large bacterial family Enterobacteriaceae, the enteric bacteria. Most of the E. coli strains are harmless but there are some serotypes of it that are pathogenic and cause serious food poisoning in human and major economic losses in both chicken and turkeys. Poultry, the second largest industry in Pakistan is supplied by lots of antibiotics like streptomycin, sulfamethoxazole and tetracycline, streptomycin and ampicillin etc, either through feed or water. Use of antibiotics at large scale is resulting in the development of antibiotic resistance in poultry and human.
Hypothesis:
To check the molecular basis of antibiotic resistance in E.coli and to find out the genotypic and phenotypic correlation between resistant E.coli from poultry drinking water.
Methodology/Parameters:
In this study, drinking water samples from poultry water were collected and cultured on MacConkey agar. Standard disk diffusion method will be used to check antibiotic susceptibility. Plasmid DNA was extracted from colonies showing antibiotic resistance by mini-prep protocol. Using universal set of primers, antibiotic resistant genes was amplified and then sequenced. The sequence thus obtained was compared in the database with previously reported sequences of antibiotic resistant gene in E. coli strains.
Statistical Design:
Prevalence of tetA and tetB genes was shown by a Column chart.
Outcomes:
This study helped to find out prevalence of these antibiotic resistance gene in E. coli isolated from poultry drinking water, which are potential threats to human being.
Availability: Items available for loan: UVAS Library [Call number: 1497,T] (1).
2.
Genetic Characterization Of Pakistani Lathy Pigeons Using D-Loop, Cyt B And 16S Rrna Genes As Genetic Marker
by Muhammad Umair Latif | Ms. Sehrish Firyal | Dr. Ali Raza Awan | Dr. Muhammad.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1399,T] (1).
3.
Diagnostic Value Of 4Bp- 5' Gtca Deletion In Duarte Galactosemia
by Sadia Zia | Dr. Muhammad Imran | Ms. Faiza | Ms. Sehrish Firyal.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1602,T] (1).
4.
Mutational Analysis Of Myelocytomatosis (Myc) Gene Isolated From Dog & Cat Tumours.
by Anum Kamal | Dr. Mhammad Wasim | Dr. Muhammad | Ms. Sehrish Firyal.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2013Dissertation note: Now a day's pets, dogs & cats form an important part of society and their health care have gain much popularity among owners. The hazardous radiations, along with other ailments cause different cancers in them which are the most fatal diseases. This increase in cancer prevalence is also related to animals living to increasingly older ages due to better nutrition, vaccination and health care provided to them. In order to provide proper treatment and care to these animal cancer patients, it is important to gather knowledge about the genes involve in causing them. There are many genes which regulate the cell cycle progression, cell proliferation and cell differentiation. Here in this study we have tried to understand this dreadful disease at molecular level. And hoping the knowledge gain through this would help in providing better treatment in future, not only to pets but also to humans. Dogs and humans share anatomical and physiological similarities, and a large number of cancers are diagnosed and managed to some extent in dogs annually. More importantly the basic biology of cancer in dogs is analogous to human cancers. According to an estimate every fourth dog suffers with cancer.
The deregulation and mutations in proto-oncogenes are the subjects under study. One such proto-oncogene is Myc, whose translated protein act as a transcription factor and regulates the expression of various genes. Myc protein binds DNA at specific sites, i-e e-box sequence and initiates the transcription of other genes, and controls their expression. Myc is assumed to regulate 15% of all cellular genes. Elevated expression of Myc is spotted in about 70% of all cancers.
The sampling was done from pet dogs and cats, belonging to different breeds with diagnosed tumor type, from the pet centre of university of veterinary and animal sciences, Lahore and various private pet clinics. 3-5 ml blood was collected aseptically and genomic DNA was extracted from blood using inorganic extraction method & its quantity was checked by NanoDrop spectophotometer. Four primer sets were designed to amplify protein coding region of Myc gene. After amplification through PCR, DNA sequencing was done. Data interpretation was done by using several softwares like BLAST alignment tool, Chromas Lite, Mega 5.2, Phyre 2, VMD 1.9.1, & PyMOL. A polymorphic change was detected in the protein coding region of Myc gene, which causes an amino acid substitution at lys355 by Arg, thus changing the Myc protein sequence. But this change might not affect protein structure much, as in some bHLH proteins Arg355 resides normally. Some changes in the 3'UTR were also detected which might play crucial part in stabilizing the Myc protein, by altering silencer box or miRNA binding sites. Thus high level of stable Myc protein causes increase cell division leading to tumor production.
This study will make available the genetic data and contribute substantial addition in the existing information in animal genetic resources. It would also aid in future to assess the possibility whether Myc can serve as a useful marker for diagnosis and prognosis of these malignancies. The need for today is to develop valid biomarkers, which can be incorporated in ongoing in vivo and in vitro clinical mechanistic and improve the diagnosis & prognosis of this dreadful disease.
Availability: Items available for loan: UVAS Library [Call number: 1604,T] (1).
5.
Analysis Of Cuticle And Ovoid Bodies In Human Hair
by Aamna Khan | Ms. Maryam Javed | Ms. Faiza | Ms. Sehrish Firyal.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1635,T] (1).
6.
Gender Differentiation From Fingerprint Ridge Count In Pakistani Population
by Ahmed Fayyaz | Prof. Dr. Tahir Yaqub | Dr. Muhammad | Ms. Sehrish Firyal.
Material type: ; Format:
print
Publisher: 2013Dissertation note: In forensic science, fingerprinting has been used for decades as an efficient tool for identification of persons linked to an illegal activity or a crime scene. Different methods for the development and analysis of the latent fingerprints have been introduced including optical, physical and chemical methods. Each method has its own importance in the development and examination of the latent prints, which are invisible to naked eye before the application of fingerprint development methods. A lot of work has been published worldwide regarding fingerprinting. It was also reported that there is a significant difference in the ridge density of males and females. Ridge count might be helpful in the gender differentiation in Pakistani population. Patent prints of 100 males and 100 females were taken on A4 size paper or card paper using pelikan black inkpad and analysis was done with the help of 10x magnification lens. The ridges were counted diagonally within a square of 5mm x 5mm. This value depicts the number of ridges per 25 mm2. Results were analyzed by using Multivariate analysis of variance (MANOVA). The results of this study are used as a helpful tool for forensic expert and law enforcement. It reveals that females have finer epidermal ridge detail than males. The degree of ridge density is used as presumptive indicator of gender of unknown print left at a crime scene. First we qualitatively examine if prints appear coarse or fine and then by quickly quantifying ridge density or ridge count in a manner similar to method described in this study. The outcomes of this study will be helpful in exoneration of innocents in different crimes.
Availability: Items available for loan: UVAS Library [Call number: 1668,T] (1).
7.
Identification Of Polymorphism In Bone Morphogentic Protein Receptor Type-1B (Bmpr-1B) In Teddy Goats
by Sonia Noreen Anjum | Dr. Muhammad Imran | Dr. Abu Saeed | Ms. Sehrish Firyal.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2013Dissertation note: Teddy goats provide a great scope for enhancing meat and milk production being the primary objective to compensate for increased demand in Pakistan. It is an established fact that an animal producing twins or triplet contributes more than 1.5 times toward meat than the animals producing single offspring per kidding. Hence, the identification of major fecundity genes, mutations of which are thought to elevate ovulation rate and litter size in goats as well as sheep breeds, has been the center of attention for all scientists. Four major fecundity genes expressed in goat ovary namely: GDF-9, BMP-15, ESR-? and BMPR-1B are the causative genes for high prolificacy.
Bone morphogenetic protein receptor type-1B (BMPR-1B) gene first identified ingranulosa cells of ovary. A-G transition at 746 bp at the FecB gene locus causing an amino acid substitution namely Q249R increases the antral follicular maturation leading to the release of a large number of ovules hence increasing litter size in range from 1.4-2.7 kids/birth.
In this study, blood samples from 52 Teddy goats were collected having twining record and processed for DNA extraction. DNA fragments containing FecB gene were PCR-amplified from extracted DNA samples. The PCR amplicons containing Q249R substitution were subjected to RFLP so that the presence or absence of these polymorphisms could be analyzed.
On analysis with DdeI restriction enzyme, three types of allelic fragments namely: wild type, homozygous mutant and heterozygous mutant of FecB gene mutation in Pakistani Teddy goats were to be observed. Whereas,the results obtained for this study strongly suggests that the Q249R mutation of FecB marker in BMPR-1B gene was not present in Teddy goats and these goats were found to be non-carriers for this mutation having wild type alleles. However, this work did not claimed the absence of any other mutation in BMPR-1B. There may be the involvement of other fecundity genescausing the increased prolificacy of these goats causing twining and triplets namely: Growth differentation factor-9 (GDF-9) and bone morphogenetic protein-15 (BMP-15).
Availability: Items available for loan: UVAS Library [Call number: 1670,T] (1).
8.
Comparasion Of Differnt Presumptive Tests For Detection Of Bloodstain After Washing Fabric With Different
by Samreen Mushtaq | Ms. Sehrish Firyal | Dr. Muhammad Imran | Ms. Faiza.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1674,T] (1).
9.
Molecular Characterization Of Antimicrobial Resistance Genes In Salmonella Isolates From Poultry
by Saba Zeb Khan | Prof. Dr. Tahir Yaqub | Dr. Muhammad | Ms. Sehrish Firyal.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2013Dissertation note: Salmonella is a gram negative bacteria which can cause a number of different diseases including gastroenteritis, bacteremia, and typhoid fever, with the most common being gastroenteritis, some serotypes of it are pathogenic and cause serious food poisoning in humans and major economic losses in both chicken and turkeys. The birds can be the reservoir of Salmonella species which cause food borne infections in human. Human get such infections by ingesting contaminated products. In poultry farms, Salmonella can be introduced by means of contaminated feeds, particularly those that contain animal raw materials.
Use of antibiotics in poultry has become a popular practice. Different antibiotics like tetracycline, streptomycin, trimethoprim etc. are given in poultry via water and feed for growth and protection against diseases. Extensive and uncontrolled use of antibiotics resulted in increased development of antibiotic resistant bacteria. Statistical data shows that Salmonella is resistant to many antibiotics especially tetracycline.
The goal of our study was Molecular characterization of tetracycline resistance genes in Salmonella spp. and to check the prevalence of tetracycline resistance genes in Salmonella isolates from poultry drinking water.
Total 50 water samples were collected from different poultry farms and poultry meat shops in Lahore district.Various biochemical tests were performed to confirm the isolated strains as Salmonella. Tetracycline resistance was examined against isolates. Plasmid DNA was extracted from these bacteria. Antibiotic resistant plasmid genes were amplified by PCR. After gel electrophoresis the resulting fragments were sequenced through genetic analyzer. After sequencing the sequence thus obtained was compared with the reported sequences of tet genes in Salmonella strains in NCBI.
It was found out that Salmonella isolates from the poultry drinking water are highly resistant to tetracycline, as 83% of the isolated Salmonella from poultry drinking water showed their resistance towards tetracycline.PCR amplification of tet genes indicated the presence of tetA gene in 100% of tetracycline resistant Salmonella, whereas 64% of the samples contained tetB gene. TetB gene was present only in combination with tetA gene. None of the sample contained tetC, tetD and tetGgene.
This study helped to find out the prevalence of antibiotic resistant genes in Salmonella isolated from poultry drinking water, which were potential threats to human being and this study will also help us in future to develop strategies to restrict the emergence of antibiotic resistant genes and their spread.
Availability: Items available for loan: UVAS Library [Call number: 1714,T] (1).
10.
Trace Analysis Of Gun Shot Residue On Different Fabrics Using Locally Manufactured Ammunition In Pakistan
by Muneeba Butt | Prof. Dr. Tahir Yaqub | Ms. Faiza | Ms. Sehrish Firyal.
Material type: ; Format:
print
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1733,T] (1).
11.
Level Of Amylase From Human Saliva Deposited On Fruit First Bite Mark
by Umar Draz | Ms. Sehrish Firyal | Dr. Mohammad Ashraf Tahir | Prof. Dr. Tahir.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2013Dissertation note: Saliva is colorless fluid which consists of epithelial cells, enzymes, non enzyme protein and inorganic components. Saliva is secreted by three glands in mouth. One is parotid gland, second is submandibular gland and third is sublingual gland. There are two types of amylases in human. One is salivary amylase, while other is pancreatic amylase. The salivary amylase is secreted by salivary gland while pancreatic amylase is secreted by pancreas. The salivary amylase is present in saliva, perspiration and breast milk. Pancreatic amylase is present in blood, feces and urine. Saliva stain is very important at crime scene for forensic investigation. Majority of techniques used for detection of saliva are based upon the presence of salivary amylase. Human saliva can serve for identification. One can extract DNA from saliva stain and generate DNA profile, whereby individual can be identified who is a source DNA profile that is generated from saliva stain. In present study level of salivary amylase was determined from human saliva deposited on fruit with first bite mark. Apple, peach and apricot were selected for this experiment. Ten males and ten females were selected to bite on fruits. The time interval was used as variable for determining the level of amylase. The time intervals were 0 hour, 12 hours, 24 hours, 36 hours and 48 hours. Samples were collected from bite mark area on fruit. The samples collected from apples and apricot pits were positive for amylase activity till 48 hours. The samples collected from peach were positive till 12 hours. The samples collected from peach were negative after 24 hours. This research indicates that salivary DNA could be found on bite mark area on apple and apricot pit till 48 hours.
Availability: Items available for loan: UVAS Library [Call number: 1755,T] (1).
12.
Estimation Of Various Adulterants In Milk Available In Local Market
by Farhan Tanveer | Dr. Muhammad Wasim | Dr. Abu Saeed | Ms. Sehrish Firyal.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1781,T] (1).
13.
Development Of The Test For The Diagnosis Of Classical Galactosemia In General Papulation
by Mehmmona Iqbal | Dr Muhammad Imran | Ms Faiza | Ms Sehrish Firyal | IBBT.
Material type: ; Format:
print
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1856,T] (1).
14.
Determination And Quantification Of P- Phenylenediamine (Ppd) From Different Brands Of Henna Available In Punjab
by Aabroo imtiaz gill | Mr.Akhtar Ali | Dr. Muhammad | Ms. Sehrish firyal.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1972,T] (1).
15.
Screening Of Indigenous Microalgae For Antimicrobial Activities And Growth Optimicrobial For Mass Production
by Imran hanif | Prof. Aftab ahmad anjum | Dr. Jawad nazir | Ms. Sehrish firyal.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2015,T] (1).
16.
Systematic And Integrated Analysis Of Bovine Mammary Gland Genes To Exokire Mastitis Resistant Genes
by Iqra Mahmood | Dr. Asif nadeem | Ms. Aisha | Ms. Sehrish firyal.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2023,T] (1).
