51.
Comparative Ealuation Of Melia Azadarch Linn (Neem) For Control Of Coccidiosis In Poultry (Broiler)
by Fouzia Iqbal | Dr. Muhammad Sabir | Dr. Mubasher | Dr. Muhammad Arshad Qureshi.
Material type: ; Format:
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; Literary form:
not fiction
Publisher: 1993Dissertation note: A study was conducted to evaluate the anti-coccidial activity of Melia azadarch Linn (Neem) , an indigenous plant drug in comparison to synthetic coccicliostats (Esb3) in broilers.
The Meli azadarch Linn (Neem) seed was tried in powder, aquous extract and methanolic extract for anti-coccidial activity in broilers. The birds were artificial infected with coccidial oocyst at the age of 30 days. After 5 days of infection the oocyst counting was carried out in the droppings of the birds. When evidence of coccidiosis outbreak in the infected birds was established than the drug was administered to different groups of the broilers. The group A was kept as control, group B, C & D were administered Powder Melia azadarch Linn (Neem) 10, 20 & 30 mg/Kg body weight respectively and group F was given Esb3, to compare the effect of this plant drug. Similarly aquous extract and Methanolic extract was administered equivalent to 10, 20 & 30 mg/Kg B.W. of Melia azadaich Linn (Neem) to respective group. The OPG (oocyst per gram) was examined after 3, 10 & 15 days after the drug administration. It was observed that OPG (oocyst per gram) was decreased to 0.59%, 0.78% and 0.58% at the dose rate of 10, 20 and 30 mg/Kg 13.W. respectively after 3 clays of treatment with Melia azadarch Linn Powder. Whereas it was reduced to 82.79% in group E treated with Esb3. After 10 days of treatment OPG was decreased to 50.17% and 96.55% in groups B, C, D & E respectively at the dose rate oF 10, 20 & 30 mg/Kg [3.W. in case of Melia azadarch Linn (Powder) and lsb1. After 15 days OPG was decreased to 97.94%, 97.08%, 97.87 and 98.60% in group B, C, D & E respectively in case of Melia azadarch Linn (Powder) at the dose rate of 10, 20 and 30 mg/Kg 13.W. and Esb3. at dose rate of 1g/liter.
In case of Melia azadarch Linn (Neern) (Water extract) OPG reduction after 3 days of treatment was 4.46%, 3,93% and
3.67% in groups B, C and 1). While OPG reduction in case of group E was 83.82%.
After 10 days of post treatment OPG counting was 5 1.33%, 51.18%, 51,10% and 96.76 in groups 13, C, 1) and E respectively. In case of OPG counting after 15 days reduction was 90.62%, 86.61%, 79.77% and 98.30% in groups 13, C, I) and l respectively. Dose rate of group B, C, & 1) is equivalent to 10, 20 & 30 mglKg B.W. and group E ig/liter i.e. Esb3:,.
In case of group treated with Melia azaclarch Linn (Neem) (methanolic extract, OPG reduction after 3 days of treatment was 0.59%, 0.78%, 0.58% and 82.79% in groups B, C, D & E respectively. In case OPG counting after 10 days of treatment was 50.17%, 50.23%, 50.17% and 96.55% in groups B, C, D & E respectively. While OPG counting after 15 clays of treatment was 97.94%, 97.08%, 97.87% and 98.60% in groups B, C, D & E. Dose rate of group B, C, D was equivalent to dose rate of 10, 20 and 30mg/kg .B.W. & group E was t ieated with I1sb at dose rate of ig/liter.
It is concluded that. Melia azadarch Linn (Neem ) is equally effective against coccidiosis in powder, aquous and methanolic extract. Although it took longer to produce similar effect as Esb3 but it is quite comparable with the results shown by 1sb. In addition the Melia azadarch linn (Neem). in either from did not show any adverse effect on the growth rate of the broilers. Any how this plant material can be used as prophylactic measures or to treat the outbreak of coccidiosis. Certainly there is a need for investigation to make this drug more efficacious.
Availability: Items available for loan: UVAS Library [Call number: 0384,T] (1).
52.
Taxonomical Studies Of Endo Parasites & Their Sequence Of Prevalence At Different Ages Of Layers
by Anwar ul Haq | Dr. Hajid Ahmad Hashmi | Dr. Mubasher Saeed Mian | Dr. Sh | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1994Dissertation note: In the present study the total infection of gastro-intestinal parasites were studied in commercial layers, maintained at various poultry farms located in and around Lahore. About 930 faecal samples were collected from those birds which were dewormed at least one month before. Birds were divided into six age groups starting from Week-i to 48.
Out of 930 faecal samples, 689 (74%) were positive for parasitic infection and 241 (26%) were negative. Ascariclia g.1ij was found in 432 (46.4%) faecal samples, Heterakis gallinarum 326 (35%) and Coccidiosis in 237 (25.4%) of the total faecal samples examined.
The age wise incidence of parasites in group A (1-8 wks) was 59.3%, in group B (9-16 wks) 66.04%, iii group C (17-24 wks) 74.1%, in group D (25-32 wks) 74.8%, in group E (33-40 wks) 88.3% and in group F (41-48 wks) 81.2% i.e. ascending order of infection. In case of seasonal prevalence of infection, the occurrence of Nematodes in June was 41.25%, in July 40.25%, in August 45.75% and in September 38.80%. The incidence of Coccidiosis in June was 27.7%, in July 24.8%, in August 16.1% and in September 28.5%. The results were recorded on the basis of E.P.G. (Egg Per Gram) of nematodes and O.P.G. (Oocyst Per Gram) of Eimeria species. In general low body weight gain and low Egg production (30-40%) in the parasitised birds as compared with non-parasitised birds were noted. Statistical analysis revealed non-significant difference between prevalence of different species of parasites in the birds.
Availability: Items available for loan: UVAS Library [Call number: 0385,T] (1).
53.
A Study On The Chemotherapy And Taxonomy Of Mange Mites In Ovine And Its Effect On Different Blood Parameters
by Rafique Rana, M | Dr. Muhammad Afzal | Dr. Mubasher Saeed Mian | Prof. Dr. SAghir | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1993Dissertation note: A study was carried out to find out the taxonomy, chemotherapy of mange mites in sheep and its effect on different blood parameters. A total of 30 flocks comprising of fifteen hundred sheep of different age and sexes were examined in the city and villages around Lahore. Out of above mentioned animals 200 suspected sheep were thoroughly examined and 30 (15%) were found positive for mange mites infestation. Species of three genera namely Sarcoptes, Psoroptes and Chorioptes were found infesting these animals.
The average number of mites recovered from the species of each genera were recorded as 851, 825 and 815 for Psoroptes, Sarcoptes and Chorioptes respectively. For the identification of different species of mange mites microscopic examination was carried out and mites were first seen under low power and then high power of magnification. The slides were mounted with mites and then examined to study their morphological characteristics.
The three genera identified were Sarcoptes, Psoroptes and Chorioptes in sheep.
Chemotherapeutic trials were carried out in suspected sheep by using two concentrations of neguvon solution i.e. 0.10% and 0.15% at ten days intervals. For this purpose sheep were divided into three groups. Group A was treated with 0.10% solution of neguvon, group B with 0.15% solution of neguvon and group C was kept as untreated control group. The data obtained on reduction of mites due to acaricide application were subjected to statistical analysis by applying completely randomized design and results were recorded. It was noticed that 0.15% neguvon was highly effective against mange mites infestation when it was replied twice after ten days interval.
The effect of mange mites on different blood parameters was also recorded. For this purpose sheep were divided into three groups. Group I comprised of clinically infested sheep, group II subclinically infested sheep and group C included healthy sheep which was kept as control for the comparison. The effect of mange mites on different blood parameters of two infested groups of sheep i.e. I and II was recorded before and after two applications of 0.10% and 0.15% neguvon solution at ten days interval and results were compared with group C for comparison.
The findings of this study indicated that the infestation of mange mites is a quite common problem in sheep population of this area. The sheep suffering from mange mites exhibit poor feed intake, severe irritation, scratching, bleeding, loss of wool, low body growth, reduced production and damaged skin. Such infested sheep are also susceptible to various viral and bacterial diseases which makes sheep production uneconomical and put extra burden on the sheep breeders.
Keeping all this in view suitable and prompt measures should be taken for early diagnosis and treatment of the infested animals. This will result not only for the control of the disease, but also help to improve the economical condition of the owners. It will also increase the gross national product of Pakistan.
Availability: Items available for loan: UVAS Library [Call number: 0386,T] (1).
54.
A Comparative Study On The Immunogenicity Of Sonicated Pasteurella Multocida And Formalized Broth Culture (Bacterin) Of Organism In Buffalo Calves
by Habib ur Rehman | Dr. Mohammad Amin Sheikh | Dr. Mubasher Saeed Mian | Dr. S.A.R. Rizvi | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1993Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0391,T] (1).
55.
Studies On The Bactgerial Causes Of Uterine Infection In Buffaloes And Cows With Special Refernce To Corynebacterium
by Asif Suleman Sahi | Dr. S.A.R. Rizvi | Dr. M. Amin Sh | Dr. Mubasher Saeed Mian | Faculty of Veterinary Sciences.
Material type: ; Format:
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; Literary form:
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Publisher: 1993Dissertation note: A total of 200 samples of uterine discharge comprising 171 buffaloes and 29 cows showing signs of endometritis were included in this study. The material was subjected to bacteriological examination.
Monoinfection was seen in case of 164 animals (141 buffaloes and 23 cows) while in case of 17 animals, mixed infection was revealed.
The relative incidence of different organisms in cows and buffaloes was calculated. Of 29 cows suffering from endometritis, 25 proved to be bacteriologically positive while of 171 samples from buffaloes suffering from endometritis 141 cases were positive due to specific organisms.
The incidence of different species of bacteria was Staphylococcus aureus 19.48 percent, Streptococcus pyogenes 7.79 percent, Streptocoçç bovis 4.32 percent, Corynebacteri urn pyogenes 23.33 percent, Corynebacterium bovis 5.19 percent, Escherichia cc ll 12.98 percent, Proteus vulgaris 9.52 percent, Pseudomonas aeruginosa 4.32 percent, Bacillus cereus 7.79 percent and Bacillus megaterium 5.19 percent.
Availability: Items available for loan: UVAS Library [Call number: 0393,T] (1).
56.
The Host Reaction To Experimentally Induced Eimeria Infections In Broilers Chicks
by Ata ur Rehman Awan | Dr. Haji Ahmad Hashmi | Dr. Mohammad | Dr. Mubasher Saeed Mian | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1994Dissertation note: The experiment was designed to study the host reaction to the experimentally induced Eimeria species in broiler chicks. For this purpose two hundred and twenty, one-day-old chicks were reared. At the age of day 3, they were divided randomly in four groups each comprising of 55 birds. They were named as A, B, C and D. The group A was kept as un-infected control, while group B, C and D, at the age of day 3, received a primary dose of 1000 virulent sporulated oocysts, through oral, intraperitoneal and subcutaneous routes respectively. The challenge doses of 50,000 virulent sporulated oocysts each were adminigtered to the members of each group B, C and D on days 13, 28 and 43 of age.
The OPG counts in group A remained nil throughout the experiment the maximum OPG counts 65,500 were recorded in group B by the day 21. On the same day OPG counts of group C and D were also on the peak found to be 40,500 and 54,500 respectively. Three mortalities occurred in group B, 4 in C and 6 in group D. Similarly the maximum weight was attained by the members of group A (Control) i.e. 1750 gm, secondly by group B (oral) i.e. 1530 gm, thirdly group D (S/C) i.e. 1410 gm and fourthly group C (I/P) i.e. 1280gm. The difference in B was of 220gm, in C 470 gm, and in D 340 gm lesser as compared to group A.
Availability: Items available for loan: UVAS Library [Call number: 0404,T] (1).
57.
A Study On The Prevalence & Taxonomy Of Biting & Sucking Lice Infestation In Sheep With Their Effect On Different Blood Parameters
by Jamal Abdul Nasir | Dr. Muhammad Afzal | Dr. Mubasher Saeed Mian | Dr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
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; Literary form:
not fiction
Publisher: 1995Dissertation note: The study was planned to determine the prevalence and taxonomy of biting and sucking lice infestation in sheep with their effect on various blood parameters. For this purpose specimens were collected from Outdoor Hospital College of Veterinary Sciences, Lahore and various private clinics in the city.
The collected specimens were preserved, stained and ide.ntified after preparing permanent mounts under the sterioscopic microscope in the laboratory. Two species of lice i.e. Damalinia (biting lice) and Liiioqnathus pedalis (sucking lice) were identified having a percentage of 10.50 and 1.0 respectively. The infestation recorded was highest during spring and autumn and lowest during the summer.
The taxonomy of the identified species were carried out by using morphological characters (Chandler, 1961 and Soulsby, 1982).
Haernatological examinations were made to compare the blood picture of healthy and infested sheep. For this purpose 20 blood samples each from healthy and infested sheep were examined. Five ml of blood was collected from the jugular vien of the sheep, with the help of disposable syringes, in a test tube having few drops of 0.1 % EDTA.
The blood parameters studied were: Haemoglobin level, Total Erythrocyte Count, Total Leukocyte Count, Packed Cell volume and differential leukocytic count. The results of haematological examination showed a significant decrease in haemoglobin, total erythrocyte count and packed cell volume, whereas a significant rise in leukocytic count and D.L.C. of infested sheep as compared to healthy sheep was observed.
The results of the present study will help in planning better control measures in order to minimize lice infestation and also aid in the eradication of various lice born diseases in sheep.
Availability: Items available for loan: UVAS Library [Call number: 0407,T] (1).
58.
Study Of Gastro-Intestinal Helminths And Taxonomy Of Species Of The Genus Paramphistomum In Camels
by Saeed A. Khan | Dr. Muhammad Afzal | Dr. Mubasher Saeed Mian | Dr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1994Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0411,T] (1).
59.
Prevalence And Chemotherapy Of Coccidiosis In Sheep And Goats And Its Effect On Certain Blood Parameters
by Nasir Ali, Faridi | Dr. Muhammad Sarwar Khan | Dr. Khalid Pervaz | Dr. Mubasher | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1995Dissertation note: This project was designed to find out the prevalance and chemotherapy of coccidiosis in sheep and goats and its effect on certain blood parameters like Haemoglobin, TLC, DLC, Glucose,and Total Serum Protein.
Two drugs namely suiphadimidine and suiphaqunoxaline were used in this research project. For this purpose 60 animals(i.e sheep and goats) were taken and divided into 3 groups(A,B and C) containing 20 animals each(i.e 10 sheep and 10 goats). Group A was again subdivided into two subgroups Ag & As containing 10 animals each(i.e 10 goats and 10 sheeps) were treated with sulphadimidine.Group B was again subdivided into two subgroups Bg & Bs containing 10 animals each(i.e 10 goats and 10 sheeps) were treated with suiphaquinoxaline. Group C was again subdivided into two subgroups Cg & Cs containing 10 animals each (i.e 10 goats and 10 sheeps) were kept as infected non.medicated control.
The efficacy of drugs were determined on the reduction of OPG in faeces.
From the results it is find out that the average OPG on zero day of medication in groups i.e Ag,As & Bg,Bs were 75100, 72200, 60800 & 67200 respectively.The average reduction of OPG on 10th day of medication in groups i.e Ag,As & Bg,Bs were 300,340,120 & 280 respectively.The OPG of control groups were increased on the subsequent days.
The average Haemoglobin on zero day of medication in groups Ag,As,& Bg,Bs were 6.53,8.02,6. 89 & 7.93 respectively The average Haemoglobin on lOtti day of medication in groups Ag,As,Bg,Ss were 8.628.80, 9.44 & 8.78 respectively.The Haemglobin of the control groups were decreased on subsequent days. Average TLC on zero day of medication in groups Ag,As,Bg,Bs were 16.50 ,16.50 & 15.50,19.50.Average TLC on 10th day of medication in groups Ag,As & Bg,Bs were 14.50,12.90 & 13.60,13.50 respectively.The TLC of control groups were increased on the subsequent days.Average DLC on zero day of medication in groups Ag was 49.40,68.00,7.00 11.10 & 17.50 in group Bg was 51.20 ,70.30,6.90, 12.20 & 19.40 in group As was 50.20,70.20,8.00,12.00 & 18.50 in group Bs was 50.40.71.60,6.10,17.20 & 18.40 .Average DLC on 10th day of medication in groups Ag was 48.40,67.00,7.00 11.10 & 17.50 in group Bg was 50.20 ,69.30,7.10,12.20 & 19.40.ln Group As was 49.40,69.10,8.00,12.00 & 18.50 in group Bs was 49.40,70.60,6.10,17.20,18.40.DLC of the control groups were incresed on the subsequent days. Average Blood Glucose on zero day of medication in groups Ag,As,Bg,Bs were 24.30, 31.30 & 29.30, 35.50 respectively.Average Blood Glucose on 10th day of medication in groups Ag,As,Bg,Bs were 37.00, 42.40, 42.80, 45.00. Blood glucose in control were decreased on the subsequent days. Average Total Serum protein on zero day of medication in groups Ag,As & Bg,Bs were 3.52,4.25,3.93,4.18. Average Total Serum protein on 10 th day of medication in groups Ag,As,Bg,Bs were 4.89,5.56 & 5.43,5.57 respectively.The total serum protein in control groups were decreased on the susequent days.
Availability: Items available for loan: UVAS Library [Call number: 0426,T] (1).
60.
Extra Articular Substitution Of The Cranial Cruciate Ligament With Patellar Tendon
by Huma Noor | Dr. Mazhar Iqbal | Dr. Mubasher | Dr. Muhammed Younas | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1993Dissertation note: Rupture of the cranial cruciate ligament is the most common cause of hind-limb lameness in the dog. Many techniques have been used for the repair of ruptured cranial cruciate ligament including intra-articular techniques both using synthetic and viable materials.
Viable materials have proved better then synthetic due to lesser chances of rejection and infection. Extra-articular techniques are also though better than intra-articular repairs as whole of the joint is manipulated in the latter which increases the chances of trauma.
In this project 12 dogs were used in which 2 were the control dogs.
The dogs underwent repair of the ruptured cranial cruciate ligament by using extra-articular technique in which a strip of the patellar ligament along with the superficial half of the patella was screwed to the lateral side of the lateral femoral condyle in a way to eliminate the cranial drawer sign. The joint was approached through a medial parapatellar artrotomy. In the control dogs the cranial cruciate ligament was cut but not repaired.
All the dogs were kept in Kennel cages for a period of twelve weeks and evaluated clinically by various physical tests at regular interval. Euthanasia was performed after twelve weeks and the stifle joints of all the operated dogs were explored for gross abnormalities. In case of the repaired dogs, majority of the results confirmed that the joints remained stable throughout the experimental period. However, in case of control dogs the post-euthanasia evaluation was clearly indicative of the complete rupture of cranial cruciate ligaments. Hence it was seen that the surgical technique used in this study provided adequate stability to the stifle joints in which the cranial cruciate ii gament was ruptured experimentally.
Availability: Items available for loan: UVAS Library [Call number: 0431,T] (1).
61.
The Effects Of Piroplasmosis On Hematology In Equines
by Salva | Dr. Mubasher Saeed Mian | Dr. Asif Rabbani | Dr. Shakeel | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1996Dissertation note: Piroplasmosis is a serious disease of the domestic animals including horses. It is caused by species of the genus Babesia i.e. . egui and B. caballi resulting in death due to excessive loss of blood. The present study was conducted to find out the effect of natural Piroplasmosis on various blood parameters, viz, total erythrocytes count (TEC), hemoglobin estimation (Jib), packed cell volume (PCV), total leukocytes count (TLC) and differential leukocytes count (DLC) in horses.
A total of forty horses (Group A including 20 uninfected horses and Group B included 20 horses showing clinical symptoms of the disease and confirmed positive for piroplasmosis through blood examination were used in the study and found to cause the infection although . caballi infection was more predominant.
Heamatological examination revealed a significant decrease (P<0.05) in the erythrocyte count, hemoglobin level and packed cell volume of the infected horses.
Normocytic and hypochromic type of anemia was observed. Total leukocyte count was also decreased. A decrease in the number of rieutrophils, eosinophils, basophils, monocytes and lymphocytes was also observed in the infected horses.
Out of the 20 infected horses, 4 had B. equi and sixteen had B. caballi infection indicating that . caballi was more prevalent.
Availability: Items available for loan: UVAS Library [Call number: 0432,T] (1).
62.
Comparative Efficacy Of Oxfendazole, Albendazole And Ivermectin Against Gastro Intestinal Nematodes Of Horses
by Nadeem Kamal | Prof.Dr.Muhammad Ashraf | Dr. Mubasher | Dr. Muhammad Sarwar Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1995Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0437,T] (1).
63.
Comparative Efficacy Of Anthelmintics Against Gastro Intestinal Parasites Of Buffalo Calves And Their Effects On Blood Parameters
by Tariq Humayun | Dr. Mubasher Saeed Mian | Dr. Haji Ahmad Hashmi | Dr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1995Dissertation note: A total of 90 (60%) out of 150 fecal samples from buffalo calves of different age groups were found positive for gastrointestinal parasites. Coprological examination revealed Toxocara vitulorurn (42.22%) as single infection and 31.11% as mixed infection. Strongy.Zoides papillosus as 21.11% as single infection arid 20% mixed infection. Nematodirus spp. 1.11% as single. Haemonchus sp., 2.22% as single and 1.11% mixed infection. Honiezia sp. 3% as single and Eimeria spp., 21% as single and 13.33% as mixed infection. Intensity of infection of gastro-intestinal parasites in buffalo calves was determined as 41.1% of calves upto 500 eggs per gm of faeces, in 22.22% calves from 501 to 1000 epg, in 16.66% calves from 1001 to 1500 epg and in 20% calves greater than 1500 epg of faeces.
Comparative percentages of means of EPG counts of infected control subgroups of groups A,B and C showed increasing trend of EPG counts by day 14. However subgroup A3, showed 'I3.l% reduction, subgroup B3 showed 61.15% reduction and C) showed 72.29% reduction in epg counts on day 3 after medication with oxfendazole. Similarly on day 7 of medication, the reduction percentage were, 95.52, 89.93 and 96.38 in respective subgroups. Albendazole in subgroups A4, 84 and C4 showed reduction on day 3, by 67.08, 64.89 and 78.57 and 95.24 respectively. On day 14, both the anthelmintics showed 100% etticacy No significant change was noted in RBC, UBC counts Hb gm/mi and PCV%, however, Eosinophils and lymphocyte counts depicted siynificant increase in the infected subgroups.
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64.
A Study Of Gastro Intestinal Helminthiasis And The Effects Of Natural Infection On Various Blood Parameters In The Buffaloes
by Ishtiaq Ahmad | Dr. Asif Rabbani | Dr. Mubasher Saeed Mian | Dr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1995Dissertation note: A total of 76 buffaloes brought to the Lahore Metropolitan Slaughter house were studied for helminthiasis. Out of which 50 (65.79%) were found positive.
The helminthiasis positive guts were examined for the presence of helminths and the following species were identified Haemonchus contortus (11.84%), Haemonchus placei (10.53%), Ostertagia ostertagi (7.89%), Trichostrongylus axei (3.95%), Mecistocirrus digitatus (2.63%), Trichostrongylus colubriformis (7.89%), Cooperia oncophora (9.21%), Cooperia punctata (5.26%), Cooperia pectinata (6.58%) , Nematodirus helvetianus (2.63%), Bunostomum phiebotomum (2.63%), Strongyloides papillosus (2.63%), Oesophagostornum radiatum (3.95%), Trichuris globulosa (1.32%), Paramphistomum cervi (3.95%), Paramphistomum microbothrium (2.63%) and Monezia benedeni (2.63%)
The effect of helminthiasis on blood parameters like, total erythrocytic count, total leukocytic count, packed cell volume, erythrocytic sedimentation rate, haemoglobin concentration was investigated and the values recorded were 5.840±0.110x106 Cimm, 5.447±0.074x103 Cumm, 32,858±0.230%, 38.004±0.217 mm/hr. and 10.368±0.096 gm/lOOml respectively. The differential leucocytic count values for neutrophils, lymphocytes, eosinophils, monocytes and basophils were
43.16±0.531%, 43.82±0.718%, 6.40±0.187%, 5.620±0.241% and 1.040±0.124% respectively.
Similarly the helminth free (control> animals studied for their total erythrocytic count, total leukocytic count, packed cell volume, erythrocytic sedimentation rate, haemoglobin concentration, gave their averages as 7.382±0.068x106 Cmm, 6.321±0.084x103 Cimm, 38.927±0.286%, 28.213±0.148 mm/hr. and 11.892±0.286 gm/lOOml respectively. The differential leukocytic count values for neutrophils, lymphocytes, eosinophils, monocytes and basophils were
29.15±0.365%, 56.0±0.513%, 8.12410.28%, 5. 852410 .264% and 0.90±0.191% respectively.
The average values for helminth infected and helminth free animals were analysed statistically. The infected animals had a significantly decreased total erythrocytic count, total leukocytic count, packed cell volume, haemoglobin concentration, lymphocytes and eosinophils with increased erythrocytic sedimentation rate and neutrophils, while no significant difference was seen in monocytes and basophils.
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65.
A Study On Gastro Intestinal Nematodes Of Camels Slaughtered At Metropolitan Corporation Abattoir Lahore With Taxonomy Of The Isolated Species
by Abbas Ali, Syed | Dr. Asif Rabbani | Dr. Mubasher Saeed Mian | Dr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1993Dissertation note: The present study was carried out to determine the incidence of gastro-intestinal nematodes of camels, with the taxonomy of the isolated species. Sixty guts were examined rendomly at the rate of 15 guts per month from August to November, 1993. The adult parasites were collected, fixed and preserved. The whole mounts were prepared. The faecal samples were examined and the EPG was estimated using McMaster egg counting technique. The taxonomy of the isolated species was determined by examing the whole mounts, according to the morphological characters given in the keys. The five species of nematodes representing different genera were isloated and identifed as Haenionchus ion gistipes, Izlaemonchus contortus, C'ainelostrongy!us mentulatus, Neniatodirus spathiger and Trichuris giobulosa.
The overall incidence recorded from camels during the present study was 65 percent. The morphological characters were studied by preparing permanent mounts and were described.
The findings of the present study will help in planning programme for the control of parasitic disease, especially caused by the above mentioned species of nematodes of camels in Pakistan, which will contribute to the development of animal industry by providing much effective coverage for the devastating effects of the parasites and will ultimately enchance the production of milk, meat and hides.
Availability: Items available for loan: UVAS Library [Call number: 0463,T] (1).
66.
Effect Of Fascioliasis On The Blood Picture And Comparative Efficacy Of Different Fasciolicidal Drugs In Sheep
by Shazia Maqbool | Dr. Khalid Pervaiz | Dr. Mubasher | Dr. Muhammad Ashraf | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1995Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0471,T] (1).
67.
A Comparative Study Of Different Anaesthetic Combinations In Equine
by Sajid Ijaz | Dr. Muhammad Arif Khan | Dr. Mubasher | Dr. Muhammad Ashraf | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1995Dissertation note: These days the scope of anaesthesia is consistently increasing due to increase in surgical patients in veterinary practice. This change in trend is due to the awareness of the owners to different surgical problems and their solutions. They do not hesitate to get their animals treated with modern equipments and highly expensive medicine. To meet this challenge and demand of the owners, veterinary surgeons are taking keen interest to get the advanced knowledge and latest information of modern equipments and their usage in veterinary practice.
In the past surgery was performed by making temporary insensibility by asphyxiation, cerebral concussion by striking wooden bowl placed over the head with a hammer and a regional anaesthesia was attained by compression of nerves and blood vessels of the region to he operated upon. But these days different sedative and anaesthetic agents are being used to make the animal desensitize from all external stimuli. These drugs are available in volatile, nonvolatile and gaseous forms. The usage of anaesthetic agent entirely depends upon the species, age, weight, temperament of the patient, nature of the operation to he performed, cost and availability of the drug.
In this project three different combinations were tried in thirty animals of equine species to find out the best selection for major surgical interventions. The animals were divided into three groups often animal each. These groups were designated as Group A, B and C. Group A was anaesthetized with XylazineiPentothal sodium, Group B with Acepromazine/Pentothal sodium and Group C with Diazepam/Pentothal sodium. The efficacy of these combinations was measured on the basis of nature of induction, recumbency period, nature of recovery, different body reflexes, recording temperature, pulse, respiration, and statistical analysis of results.
The analysis of the results collected during this study clearly indicated that a combination of cliazepam with pentothal sodium was a better selection for anaesthetizing equine patients. This study aslo proved that combination comprising of xylazine and pentothal sodium could he used for minor surgical interventions as it produced light surgical anaesthesia.
It was concluded that a combination consisting of diazepam and pentothal sodium should be used for all types of major surgical procedures in equine practice.
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68.
Comparative Efficacy On Newly Introduced Antibacterial Drugs Against Experimentally Induced Escherichia Coli
by Zubair, M | Dr. Khalid Pervaiz | Dr. Mubasher | Dr. Muhammad Sarwar Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1996Dissertation note: Pakistan faces an acute shortage of animal protein which is acknowledged as a serious problem at national level. Since broilers are the fastest converters of crude protein into digestible high quality protein for human consumption. There is much need in the production of broilers for the said reason in Pakistan. The upgrading of poultry industry is suffered from huge losses caused by certain diseases of bacterial origin. Amongst bacterial diseases, Escherichia coli is the worst threat to poultry industry. Therefore, concrete and consistent effort was done in this project to control the Eschericia coli infection.
The present project was done to study the efficacy of different drugs on prevalent strains of E. coli isolated from broilers that were collected from Diagnostic Laboratory, office of the Project Director, Poultry Production, 16-Cooper Road, Lahore and various Farms in the vicinity of Lahore.
Six different antibacterial drugs which comprised of avitryl, advocin, plasmacolin, flumequine gentamicin and inoxyl were used to check the sensitivity of the isolates. In-vitro the sensitivity results was in this order avitryl, advocin, plasmacolin, gentamicin, flumeuine and inoxyl respectively. Avitryl emerged as best drug.
The pathogenicity of the isolated strains was tested in albino mice taken from V.R.I., Lahore. The confirmed pathogenic isolates of . coli were diluted upto 3x108 bacteria per 0.25 ml, this dilution ws made after doing total viable count and the dose was injected intra-peritoneally to five albino mice while 5 albino mice were kept as controls.
These six drugs were further tested for their efficiency in broilers against . coli infection. A total of two hundred and forty birds reared upto four weeks of age and then they were divided into eight groups, A, B, C, D, E, F, G and H, each having 30 birds. Group H was kept as non-infected and non- treated. While Group G was infected but not treated. The remaining groups A, B, C, D, E and F, 16 hours after infection were treated with avitryl, advocin, plasmacolin, flumequine, gentamicin and inoxyl for five days respectively. All the groups were kept under close observation and their mortality and feed efficiency was noted.
In group G maximum mortality was noted i.e. 57.14 while in the group A, B, C, D, E and F, the mortality recorded was 22.72, 30, 33.33, 40.90, 47.82 and 52.17 respectively.
Avitryl was proved to be the highest protection against coli infection and best in relation to F.C.R. by this experiment.
Availability: Items available for loan: UVAS Library [Call number: 0492,T] (1).
69.
Comparative Nutritive Value Of Corn Gluten Meal And Meat Meal In Broiler Rations
by Syed Tayyab Waqar Bokhari | Dr. SAghir Ahmad Jafri | Dr. Mohammad Yaqoob Malik | Mrt. Mubasher.
Material type: ; Format:
print
; Nature of contents: ; Literary form: Publisher: 1985Dissertation note: The experiment was conducted in the Animal Nutrition Section, College of Veterinary Sciences, Lahore on completely randomized design. One hundred and twenty, day-old broiler chicks were reared for eight weeks period, These were divided into four groups of 30, and each group was further divided into three replicate of 10 birds. Four experimental rations A, B, C and D were fed to the four different groups. There rations contained 0, 10,15 & 20% levels of corn gluten meal with 18, 9. 5,5 & 0% levels of meat meal as sole source of protein. Watering and feeding was done a libitum.
At the end of experiment, the results showed that average body weight gain of individual bird fed on rations A, B, C and D was 322.2, 263.8, 352.7 and 252.8 grams with F.C.R. of 2.35, 2.61,2.30 & 3.04 respectively. The data regarding the body weight gain and F. C. R. was subjected to statistical analysis - using the analysis of variance and further more to find out the difference in treatment means. Student Newman Keul's Technique was applied. The results showed the highest gain in body weight in birds of group C (15% corn gluten, 5% meat meal and 0. 5% L-Lysine) and the least gain in weight in group D (20% corn gluten and 0.5% L-Lysine. Similar results in case of F. C. R. were obtained as well.
The data on dressing percentage of birds fed rations A, B, C and D were 74.5, 72.1, 71.6 and 72.4 percent. Statistically these results found to be significant and the maximum dressed body weight was observed in group A. The gizzard weight of the birds kept on rations A, B, C and D was 126. 0, 08.3, 120.7 and 105.7 grams with 123.7, 92.7, 100.0 and 94.3 grams of liver weight respectively were observed. The statistical analysis showed a significant difference. Improved weight of gizzard and liver were gained by the birds maintained on ration A. The data on heart, spleen and pancrease weights on analysis revealed a non- significant difference.
The data on economics of the experimental rations indicated that the cost per Kg. of gain in body weight was Rupees 10. 52, 8.73, 8.57 and 11.16 respectively. The ration C was comparative by more economical, ration B being second the best. It is evident from the data that 15% corn gluten with 5% meat meal helped in making the ration most economical.
Based on the observations cited above it was inferred that corn gluten meal could be successfully incorporated in broilers ration without any deteriorating effect o growth rate, feed consumption or feed efficiency, provided it is supplemented with animal protein source to make up the limiting amino-acids.
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70.
Study Of Pathogenesis Of Mycoplasma Gallisepticum In White Leg Horn Layer
by Mubasher Rauf | Prof.Dr.Zafar Iqbal Ch | Dr Aftab | Dr.M.Younus Rana.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2010Dissertation note: In first part of present study 380 samples were collected from clinically suspected cases of layers suffering from respiratory diseases in and around Lahore. Samples were subjected for mycoplasma isolation by using Frey's medium. Plates with positive growth revealed characteristic colonies on 8th day post inoculation that reached maximum in size and growth at 15th day post inoculation. Out of 380 samples, 104 (27.36%) samples were positive on culture. Isolates were identified through growth inhibition test (GIT) by using hyper immune sera raised in rabbits. Isolates were further confirmed by PCR. Similarly, tracheal swabs and tissue samples of lungs and trachea collected under refrigeration were also subjected for DNA analysis. Out of 380 samples 264 (69.5%) were positive on PCR analysis. By comparing two diagnostic techniques it was found that PCR was more sensitive and reliable technique for screening of Mycoplasma gallisepticum.
In second part of study experiment isolates were analyzed for protein profile of Mycoplasma by standardization of two techniques Sodium dodecyl sulphate polyacrylamide gel (SDS-PAGE) and western blotting. These techniques help to find out any antigenic variation in prevailing strain of mycoplasma. During our study five bands of protein were detected with molecular size of 32.35 kDa, 43.65 kDa, 52.48 kDa, 64.56 kDa and 70.8 kDa. These proteins were extracted from whole cell of Mycoplasma gallisepticum isolates. On comparing the molecular sizes it was found that isolated species showed low antigenic variation, analyzed by SDS-PAGE. Western blot was used to determine the specific protein of Mycoplasma gallisepticum with the use of specific polyclonal antibody raised in rabbit. The positive reaction site was shown on nitrocellulose membrane confirming target species of CRD.
During third part of present study, it was concluded that aerosol route of infection causes early disease, followed by intra tracheal and per-oral route respectively. The severity of infection was found more in aerosol and intra tracheal routes of inoculation than per-oral route which was found to be very mild. The general gross lesions observed in the above two groups were hemorrhages in trachea with mucous plug. There was air sacculitis, hemorrhages in the lungs, salpingitis and putrefied eggs in the ovary. On histopathological examination lesions were found in trachea, lungs and oviduct.
Re-isolation was carried out to confirm antigen in experimentally inoculated birds. Paraffin embedded sections of trachea, lungs and oviduct were processed for immunohistochemical examination in order to confirm the antigen of Mycoplasma gallisepticum within tissue. A positive immunochemical reaction was found in lungs and oviduct. Which represents that antigen was same as inoculated during study of pathogenesis.
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71.
Identification Of Potential Drug-Drug Interactions In Prescriptions Dispensed By Community Pharmacies In The Urban
by Muhammad Mubasher | Ms. Huma Rasheed | Muhammad Irfan Masood | Prof. Dr.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2011Dissertation note: Pakistan is a developing country of South Asia and health care status of the people is considerably low compared to the developed countries of World. The concept of rational prescribing is still not fully understood by health care professionals of this region of the world. This study was designed to identify the most frequently encountered potential DDIs in prescriptions dispensed in community pharmacies in Lahore. A total of 1554 DIs were identified in 655 prescriptions out of 1000 analyzed prescriptions. The identified drug interactions were classified on the basis of their type, mechanism and outcome, severity, onset, and documentation status. It was observed that alcohol-drug interactions 582 (37.45%) and DDIs 524 (33.72%) are the most frequently occurring drug interactions in our society. Although most of the identified DDIs were moderate 233 (44.38%) in severity having delayed onset 230 (43.89%) and possible documentation 214 (40.84%), incidence of rapid onset 171 (32.63%), major DDIs 88 (16.76%) was also alarming in prescriptions dispensed at community pharmacies of urban Lahore. Aspirin was the most frequently interacting drug 138 times (26.34%) and acetaminophen-orphenadrine combination with 53 (10.91%) encounters was the top interacting combination followed by aspirin-clopidogrel combination. The incidence of DDIs increased significantly with increase in the number of medicines (r value = 0.87) in a prescription. On the basis of findings, recommendations that how potential DDIs can be avoided were made.
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