1.
Application Of Indirect Haemagglutination (Iha) Test For The Confirmation Of Natural Hydatidosis In Cattle
by Ramiz Hussain Farooqui | Mubasher Saeed Mian | Asif Rabbani | Tufail Muhammed | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1987Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0026,T] (1).
2.
A Study On The Prevalence And Pathogenicity Of Tick Infestation With Taxonomy Of The Genus Rhipicephalus In Dogs
by Azmat Sultana | Mubasher Saeed Mian | Asif Rabbani | Muhammed Sarwer khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1990Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0032,T] (1).
3.
Study On Gastro-Intestinal Nematodes And Taxonomy Of The Species Of The Genus Haemonchus In Sheep And Goats
by Abid Naseem | Asif Rabbani | Mubasher Saeed Mian | Muhammed | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1986Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0100,T] (1).
4.
The Efficacy Of Tetramisole, Mebendazole And Pyrantel Pamoate Against Natural Infectgion Of Toxocara Canis In Dogs
by Shahid Umar, S | Asif Rabbani | Mubasher Saeed Mian | Tufail | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1985Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0118,T] (1).
5.
Effects Of Natural Dicrocoeliasis On Different Blood Parameters & Serum Bilirubin Level In Sheep
by Alia Bano | Mubasher saeed Mian | Asif Rabbani | Manzoor Ahmed Basra | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1991Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0235,T] (1).
6.
Haematological Studies, Serum Bilirubin Determination, Chemotherapy And Incidence Of Fascioliasis In Naturally Infected Sheep
by Ajaz Saleem, M | Mubasher saeed Mian | Asif Rabbani | Iqbal ahmed | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1985Dissertation note: A study was conducted to find out the haematological changes, serum bilirubin level, relative efficacy of the three drugs, i.e., Coriban (Wellcome), Bilevon-M (Bayer) and Acedist (Ciba) and incidence of fascioliasis in naturally infected sheep. The study was made from the first week of January to the last week of March, 1985.
Haematological changes were observed after examining the Total Erythrocyte Count, Total Leucocyte Count, Differential Leucocyte Count, Packed Cell Volume and haemoglobin estimation of 80 naturally infected and 20 healthy sheep in Lahore. It was concluded that there was a drop in erythrocyte count, packed cell volume and haemoglobin concentration in the infected sheep and a normocytic normochromic type of anemia was detected. Besides this, it was also noticed and recorded that there was a rise in the total serum bilirubin level of the same 80 infected sheep which were naturally infected with fascioliasis were divided into four groups A, B, C and D of 20 animals each for the study of the efficacy of three antifascioliatic drugs (Coriban, Bilevon-M and Acedist) at manufacturer's recommended dose rates afterwards. Each group was given a separate drug on the Zero day and the fourth group (D Group) was left untreated as a Control At the end of experiment on the 28th day, the efficacies recorded were 98.86%, 97.67% and 97.70% for Coriban, Bilevon-M and Acedist respectively. Statistically there was no significant difference between the three drugs but a significant difference between the replications was noted by applying analysis of variance. An increase in the daily average weight gain of 48.21, 35.71 and 39.28 grams were recorded in A, B and C groups respectively and by applying paired 't' test it was proved that there was a significant difference between the weight gains of the animals treated with these drugs.
100 livers along with gall bladders were randomly selected from different abbatoirs of Lahore during the months of January, February and March to study the incidence of fascioliasis in sheep. An incidence of 8% was recorded for Fasciola hepatica and 4% for Fasciola gigantica, with an overall percentage of 12. for fascioliasis in sheep.
Availability: Items available for loan: UVAS Library [Call number: 0258,T] (1).
7.
Comparative Studies On The Efficacy Of Indirect Haemagglutination Test (Iha) And Double Immuno Diffusion Test (Did)
by Zulfiqar, M | Mubasher Saeed Mian | Asif Rabbani | Khalid Pervaiz | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1985Dissertation note: One hundred faecal samples, 100 blood samples and livers including bile ducts were collected from sheep suffering from Fascio1iasis slaughtered at Lahore and Sheikhupura abbatoirs.
Faecal examinations were carried out with fresh smear and centrifugal techniques and only 35 animals were found passing Fasciola ova. Serum was prepared from the blood samples collected from infested sheep for performance of indirect hacinagglutination test and double imniuno-diffusion test. Crude antigen was prepared from the fresh liver flukes specimens collected from infected livers which contained 4.5 mg protein/mi. Hyper immuneerim was prepared in rabbits by inoculating antigen. Double immuno-diffusion test was performed on glass slides coated with 1% agar Gel and. incubated at 37 C for 48 hours. The positive reactions showed diffuse lines. 45 sheep were found positive with Double immuno-diffusion test.. For performing the indirect haemagglutination test fresh red blood cell of the healthy sheep were stabilized with gluteraldehyde and sensitized with Tannic acid. Crude antigen was attached to these cells at pH 6.4 which were used in Indirect haemagglutination test. Serum samples were tested with Fasciola antigen in "V" plates of microtiter sptem. The end points of the plates were read after 16 hours a room temperature. Sora were prediluted 50 times but agglutination was seen even at 1/100 dilution. In positive reactions cells were like mat of cells in the bottom of the wells In negative reactions, cells settle down and looked like button in the center. 59 animal were found positive with indirect haemagglutination test. While comparing the sheep serum samples results with hyper immune serum collected rom rabbits it was found that all the positive sheep serum samples coincide with the results of hyper immune serum.
From the conclusion of the results it was found that indirect haemagglutination test was more sensitive (59%) than double immuno diffusion test(45%)as compared with the coprological examination 35%
Availability: Items available for loan: UVAS Library [Call number: 0272,T] (1).
8.
A Study On The Taxonomy & Prevalence Of The Species Of Genus Ancylostoma & Effects On Natural Infections On Various
by Kamran Ashraf | Mubasher Saeed Mian | Asif Rabbani | Muhammed Sarwer Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1993Dissertation note: The study was conducted to determine the prevalence of Ancylostomes (Hookworms) in dogs, taxonomy of the species of the Genus Ancylostoina and their effects on various blood parameters of the infected canines.
For above purpose, samples were collected from dog hospital, College of Veterinary Sciences and different pet clinics in the city.
Faecal and blood samples were stored in the refrigerator, of Parasitology Laboratory.
Only those dogs which had high prevalerit in Ancylostomes were examined by different faecal examination methods, were selected to get mature ancylostomes, after killing the dogs. Preservation and staining of Ancylostimes was carried out by adopting the prescribed methods. The taxonomy of the species of the genus Ancylostoma was studied with the help of keys.
The collective over all prevalence of different types of Ancylostomes recorded in stray and pet dogs was 28%.
The species of Ancylostomes identified in our area were: Ancylostoina Caninum, Ancylostoma braziliense and Ancylostoma duodene1e.
The percentage of the above mentioned species were 100%, 33% and 2.5% respectively. Blood of infected dogs were exposed to various haematological studies including Hb estimation, erythrocyte sedimentation rate (ESR), packed cell volume (PCV), total erythrocyte count (TEC), total serum protien level (TSP) and differential leukocyte count (DLC).
liaematological examinations were made to compare the blood picture of healthy and infected dogs. For this purpose 20 blood samples each from healthy and infected dogs were examined.
The results of haematological examination showed a significant decrease in haemoglobin estimation total erythrocyte count, packed cell volume and total serum protien level, whereas a significant increase in erythrocyte sedimentation rate and differential leukocyte counts, like neutrophils, eosinophils and very little increase in basophils were also seen.
Availability: Items available for loan: UVAS Library [Call number: 0284,T] (1).
9.
Efficacy Of Coxeva In Experimental Caecal Coccidiosis And Its Effects On Body Weight In Broiler Chicks
by Yaqub Malik, M | Mubasher Saeed Mian | Asif Rabbani | Muhammed Sarwer Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1993Dissertation note: The study was conducted to evaluate the efficacy of anticoccidial drug coxeva (a mixture of 80% sulphadimidine and 8% diaveridine) and its effects on body weight, growth rate and feed convertion ratio were studied. For this purpose 120 day old broiler chicks were reared upto the age of 4 weeks. At the end of 4 weeks the birds were randomly divided into four groups A, B, C, and D, each group containing 30 chicks. The birds of each group were weighed. The group B, C and D were infected with 50,000 sporulated oocysts of Eimeria tenella. While the group A was left uninfected and kept as control on the 6th day after the appearance of clinical symptoms of caccal coccidiosis, the group C and D were medicated with coxeva (the group C for five continuous days and group D for three days on 2 days off three days on), the group B was left as infected control. The daily oocysts count per gram of faeces were taken upto the end of experiment which was zero in group C on 10th day and in group D on 13th day the oocysts were at their peak (5,20,000) on 15th day of inoculation. The body weight was recorded at the end of each week throughout the experiment which showed significant difference in group B and group A, C and D . The feed consumed by each group was recorded on daily basis and growth rate was recorded at the end of each week. The analysis of variance revealed significant difference in feed consumption and growth rate in group B and other groups. The feed conversion ratio was recorded for each group at the end of each week which showed non significant difference among group A, B, C and D. The drug was also evaluated for its modes of administration (continuousand intermittent) and found no significant difference in both modesof administration. The mortaility was recorded which was five birds (16%) in group B. The drug proved hundred percent effective against caecal cocidiosis in broiler chicks.
Availability: Items available for loan: UVAS Library [Call number: 0345,T] (1).
10.
Serodiagnosis Of Ovine Hydatidosis
by Javaid, M | Dr. Asif Rabbani | Dr. Mubasher Saeed Mian | Dr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1993Dissertation note: A study was under taken to find out the incidence of hydatidosis and to evaluate the efficacy of indirect haemagglutination (IHA) test for the confirmation of natural hydatidosis in sheep slaughtered at Lahore municipal abattoir.
Blood samples from 200 sheep (50 each from hydatidosis affected and free sheep on the basis of postmortem findings and 100 blood samples Elected randomly without considering postmortem finding). The serum from each sample was separated, properly labelled and stored at -20°C.
For the preparation of antigen, crude cyst fluid was aspirated aseptically from hydatid cysts. Blood from healthy sheep was collected in 3.8% sodium citrate solution and red blood cells were separated by centrifugation. A 2.5% red cell suspension was prepared in Phosphate Buffered Saline (PBS). The sheep erythrocytes were sensitized by Hydatid fluid antigen. Optimal dilution of antigen 1:16 was used in Phosphate Buffered Saline (p11 6.4) for sensitizing the sheep erythrocytes. All the sera were inactivated at 56°C for half an hour and serial two f1d serum dilutions were prepared by micropipettes in microtitre U plates and sensitized erythrocytes were added to the plates and incubated at room temperature in a humid chamber for 3 hours. A titre of 1:32 and above was considered as positive. In positive reactions, the cells agglutinated like a carpet at the bottom of the wells where as in negative cases the cells settled as a compact mass in the centre of the wells. By the indirect haemagglutination test Ninety-two percent sheep were found positive for hydatidosis. (Table-4.2). Out of 50 serum samples (Group A2), 46 (92%) were confirmed positive on postmortem while 3 out of 50 (6%) hydatid free samples (Group A3), gave false positive results with IHA test.
It was concluded that indirect haemagglutination test is an accurate, reliable and sensitive test (92%) for the diagnosis of hydatidosis in sheep.
The blood cell counts (TLC, DLC) and blood chemistry (Total protein, A/G ratio) of the samples under investigation were also carried out. From the results it was evident that the hydatid cysts did not affect the blood values of the host significantly. However, only 28% of hydatid positive animals showed eosinophilia ranging from 7 to 23% which was non pathognomonic.
It was thus inferred that blood cell counts and blood c1vmistry of the hydatid cyst patients was of no diagnostic value.
Availability: Items available for loan: UVAS Library [Call number: 0359,T] (1).
11.
The Effects Of Piroplasmosis On Hematology In Equines
by Salva | Dr. Mubasher Saeed Mian | Dr. Asif Rabbani | Dr. Shakeel | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1996Dissertation note: Piroplasmosis is a serious disease of the domestic animals including horses. It is caused by species of the genus Babesia i.e. . egui and B. caballi resulting in death due to excessive loss of blood. The present study was conducted to find out the effect of natural Piroplasmosis on various blood parameters, viz, total erythrocytes count (TEC), hemoglobin estimation (Jib), packed cell volume (PCV), total leukocytes count (TLC) and differential leukocytes count (DLC) in horses.
A total of forty horses (Group A including 20 uninfected horses and Group B included 20 horses showing clinical symptoms of the disease and confirmed positive for piroplasmosis through blood examination were used in the study and found to cause the infection although . caballi infection was more predominant.
Heamatological examination revealed a significant decrease (P<0.05) in the erythrocyte count, hemoglobin level and packed cell volume of the infected horses.
Normocytic and hypochromic type of anemia was observed. Total leukocyte count was also decreased. A decrease in the number of rieutrophils, eosinophils, basophils, monocytes and lymphocytes was also observed in the infected horses.
Out of the 20 infected horses, 4 had B. equi and sixteen had B. caballi infection indicating that . caballi was more prevalent.
Availability: Items available for loan: UVAS Library [Call number: 0432,T] (1).
12.
A Study Of Gastro Intestinal Helminthiasis And The Effects Of Natural Infection On Various Blood Parameters In The Buffaloes
by Ishtiaq Ahmad | Dr. Asif Rabbani | Dr. Mubasher Saeed Mian | Dr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1995Dissertation note: A total of 76 buffaloes brought to the Lahore Metropolitan Slaughter house were studied for helminthiasis. Out of which 50 (65.79%) were found positive.
The helminthiasis positive guts were examined for the presence of helminths and the following species were identified Haemonchus contortus (11.84%), Haemonchus placei (10.53%), Ostertagia ostertagi (7.89%), Trichostrongylus axei (3.95%), Mecistocirrus digitatus (2.63%), Trichostrongylus colubriformis (7.89%), Cooperia oncophora (9.21%), Cooperia punctata (5.26%), Cooperia pectinata (6.58%) , Nematodirus helvetianus (2.63%), Bunostomum phiebotomum (2.63%), Strongyloides papillosus (2.63%), Oesophagostornum radiatum (3.95%), Trichuris globulosa (1.32%), Paramphistomum cervi (3.95%), Paramphistomum microbothrium (2.63%) and Monezia benedeni (2.63%)
The effect of helminthiasis on blood parameters like, total erythrocytic count, total leukocytic count, packed cell volume, erythrocytic sedimentation rate, haemoglobin concentration was investigated and the values recorded were 5.840±0.110x106 Cimm, 5.447±0.074x103 Cumm, 32,858±0.230%, 38.004±0.217 mm/hr. and 10.368±0.096 gm/lOOml respectively. The differential leucocytic count values for neutrophils, lymphocytes, eosinophils, monocytes and basophils were
43.16±0.531%, 43.82±0.718%, 6.40±0.187%, 5.620±0.241% and 1.040±0.124% respectively.
Similarly the helminth free (control> animals studied for their total erythrocytic count, total leukocytic count, packed cell volume, erythrocytic sedimentation rate, haemoglobin concentration, gave their averages as 7.382±0.068x106 Cmm, 6.321±0.084x103 Cimm, 38.927±0.286%, 28.213±0.148 mm/hr. and 11.892±0.286 gm/lOOml respectively. The differential leukocytic count values for neutrophils, lymphocytes, eosinophils, monocytes and basophils were
29.15±0.365%, 56.0±0.513%, 8.12410.28%, 5. 852410 .264% and 0.90±0.191% respectively.
The average values for helminth infected and helminth free animals were analysed statistically. The infected animals had a significantly decreased total erythrocytic count, total leukocytic count, packed cell volume, haemoglobin concentration, lymphocytes and eosinophils with increased erythrocytic sedimentation rate and neutrophils, while no significant difference was seen in monocytes and basophils.
Availability: Items available for loan: UVAS Library [Call number: 0445,T] (1).
13.
A Study On Gastro Intestinal Nematodes Of Camels Slaughtered At Metropolitan Corporation Abattoir Lahore With Taxonomy Of The Isolated Species
by Abbas Ali, Syed | Dr. Asif Rabbani | Dr. Mubasher Saeed Mian | Dr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1993Dissertation note: The present study was carried out to determine the incidence of gastro-intestinal nematodes of camels, with the taxonomy of the isolated species. Sixty guts were examined rendomly at the rate of 15 guts per month from August to November, 1993. The adult parasites were collected, fixed and preserved. The whole mounts were prepared. The faecal samples were examined and the EPG was estimated using McMaster egg counting technique. The taxonomy of the isolated species was determined by examing the whole mounts, according to the morphological characters given in the keys. The five species of nematodes representing different genera were isloated and identifed as Haenionchus ion gistipes, Izlaemonchus contortus, C'ainelostrongy!us mentulatus, Neniatodirus spathiger and Trichuris giobulosa.
The overall incidence recorded from camels during the present study was 65 percent. The morphological characters were studied by preparing permanent mounts and were described.
The findings of the present study will help in planning programme for the control of parasitic disease, especially caused by the above mentioned species of nematodes of camels in Pakistan, which will contribute to the development of animal industry by providing much effective coverage for the devastating effects of the parasites and will ultimately enchance the production of milk, meat and hides.
Availability: Items available for loan: UVAS Library [Call number: 0463,T] (1).
