1.
Genotyping Of Hydatid Cyst And Itd Prevalence In Cattle,Buffalo And Human Beings
by Muhammad Nauman Zahid | Prf.Dr. Azhar Maqbool | Dr.Aftab | Dr.kamran Ashraf | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Nature of contents: ; Literary form: Publisher: 2008Dissertation note: ACystic echinococcosis (CE) is the larval cystic stage (called echinococcal cysts) of a small taeniid-type tapeworm (Echinococcus granulosus) that may cause illness in intermediate hosts, generally herbivorous animals and people who are infected accidentally. Echinococcus granulosus has number of genetically distinct strains which are known to differ morphologically and epiderniologically. Out of 150 cattle and 150 buffalo examined only 42 Samples of hydatid cysts were collected from different organs i.e. livers, kidneys, lungs and hearts from Lahore abbatoir. From 42 positive samples, 25 cysts were found in cattle and 17 cysts were tound in buffalo. Prevalence of hydatidosis in cattle was recoreded as 16.66% and 11.33% in buffalo. Fertility and viability of the cysts was observed microscopically. Out of 25 cysts of cattle. nine were fertile and out of 17 cysts of buffalo, only five were fertile.
Seroprevalence of hydatidosis in 150 butchers working in abattoir was also determined by the use of Latex agglutination test (LAT) kit for detection of hydatidosis.
The prevalence of Echinococcus is 24% which was derived from serum analysis of butchers.
DNA from hydatid cyst was extracted. Polymerase Chain Reaction was run on extracted DNA samples. Amplicon was run on 1% agarose for confirmation of size and specificity of product. Size of PCR product was approximately l300bp.
Genotyping of Echinococcus granulosus was performed through Polymerase Chain Reaction- Restriction Fragment Length Polymorphism (PCR-RFLP). The PCR-RFLP analysis of CO I gene of Echinococus was performed to confirm the strain of Echinococcus in cattle .The data obtained was analysed and it was concluded that the G5 strain of echinococus is prevalent in Cattle in Punjab area.
It is hoped that the findings of the present study will be helpful for further planning about the control of the disease and correlating the prevalence in cattles,buffalos and butchers from the zoonotic point of view.
According to the results, the PCR-RFLP analysis of samples of patients suspected for Echinococejis is a promising diagnostic method and also confirms the type of Echinococcits prevalent in that area and also enables an early direct detection of parasite DNA. This effort is a step to minimize the losses produced by this disease.
Availability: Items available for loan: UVAS Library [Call number: 1097,T] (1).
2.
Comparative Growth Performance Of Nili-Ravi Buffalo Calves Raised On Three Different Liquid Feeds
by Muhammad Nauman.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2111,T] (1).
3.
Optimization Of Loop-Mediated Isothermal Amplification (Lamp) For The Molecular Diagnosis Of Feline Babesiosis
by Muhammad Awais Salim (2012-va-606) | Dr. Muhammad Lateef | Prof. Dr. Azhar Maqbool | Dr. Muhammad Nauman Zahid.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: Babesia is a worldwide tick borne hemoparasite causing Babesiosis, an important disease affecting a number of animals and attracting the researcher’s attention due to its zoonotic potential.Babesiosis in cats often presents as a chronic and low grade disease, however most common symptoms include anaemia, lethargy, weakness and rarely icterus and fever.
Blood samples were collected from 100 domestic cats at Pet Center, UVAS, Lahore,from their ear tips and cephalic/saphenous vein. The blood will be immediately transferred to EDTA coated vacutainers. Stained thin blood smears were observed for intra-erythrocytic bodies and 45 samples were selected after screening. Blood in EDTA were tested for PCR (already optimized) in the Molecular Parasitology laboratory at UVAS, Lahore, to screen for B. felis. ExtractedDNA of confirmed B. felis samples were further processed forLoop-Mediated Isothermal Amplification (LAMP).
LAMP primers weredesigned recognizing four sections of the B. felis gene. LAMP reactions of 25µL were standardized at 60°C temperature for 1 hour time using DNA extracted from blood samples of cats found positive on PCR.
Briefly, the concentration of FIP and BIP were varied from 0.8µM to 2.4µM, Mg2+from 2mM to 4mM, betaine from 0.2 to 0.8M and dNTPs from 1mM to 4mM.The LAMP reaction was optimized at the final concentration of 0.2µM F3 and B3, 2.0µM of each of the FIP & BIP, 2mM for each dNTPs, 0.8M betaine, 1X reaction buffer, 1µl bst polymerase and 2µl DNA templates at 60°C.
Availability: Items available for loan: UVAS Library [Call number: 2374-T] (1).
4.
Poultry Waste Management And Its Impact on Public Health In Lahore, Punjab, Pakistan
by Muhammad Nauman Akhtar (2006-VA-150) | Dr. Hassan Mushtaq | Prof. Dr. Mansur-ud-Din Ahmad | Dr. Muhammad Ijaz.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: Theses submitted with blank cd. Availability: Items available for loan: UVAS Library [Call number: 2383-T] (1).
5.
Evauation Of Antioxident And Antimicrobial Potential Of Licorice To Enhance Microbial Safety Of Chicken Meat Patties
by Muhammad Nauman Aslam (2011-VA-473) | Dr. Muhammad Sohaib | Dr. Azmat ullah khan | Dr. Sher Ali.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: Safety of processed meat products is one of the challenging issues for the food processing industry. Meat is a perishable commodity and can be spoiled easily from microbial growth as well as chemical deterioration. To enhance the safety and shelf life, synthetic additives are used for maintaining quality of processed meat products. As, these synthetic additives pose toxic and carcinogenic health hazards to human therefore, consumers demand safe, natural and healthy alternatives of these synthetic counterparts. Herbs and spices have ability to act as source of ntural antioxidant & antimicrobial agent and licorice is among one of them. Hypothesis of this study was that added licorice extract can improve the antioxidant as well as microbial safety of meat patties at various storage intervals.
In present study, commercially available chicken meat used to prepare meat patties and licorice extract were used in different treatments during mincing. After product development, patties were cooked followed by storage at refrigeration temperature for analysis at 0, 3rd and 7th day of storage. Lipid oxidation, quality parameters, sensory evaluation and microbial analysis were conducted for detection of oxidation and storage stability. The data was analyzed through two factor Analysis of variance using 2 factor factorial design was applied. ANOVA test was used to estimate the significance of results. Tuckey test was used for the comparison of the Means as per requirements using SPSS software to estimate the level of significance.
The results of study explored that licorice is beneficial for inhibiting the oxidation in chicken meat patties as results of TBARS showed significance difference among control, BHT and licorice enriched treatment samples. Also the T4 (Group containing 1 g/kg of licorice extract and T5 (Group containing 1.25 g/kg of licorice extract) showed most promising results regarding
Summary
61
the antioxidant activity in chicken meat patties. Similarly, current study showed the efficacy of licorice against bacteria. Specially the T4 (Group containing 1 g/kg of licorice extract and T5 (Group containing 1.25 g/kg of licorice extract) and Results of Total Plate count showed positive results as an antibacterial agent in chicken meat patties. While the color of chicken meat patties (parameters L*, b* and a*) was estimated using minolta meter and as expected the results of T4 (Group containing 1 g/kg of licorice extract and T5 (Group containing 1.25 g/kg of licorice extract) was more redish and little light as compared to the control sapmle containing no antioxidant. Sensory evaluation of chicken meat showed that overall acceptability of chicken meat patties containing the licorice extract in high dose as in T4 (Group containing 1 g/kg of licorice extract and T5 (Group containing 1.25 g/kg of licorice extract) had highest values compared to the control and BHT containing chicken meat patties.
Outcomes and suggestions
The utilization of licorice extract as a source of natural antioxidant as well as antimicrobial agent in processed chicken meat products. It provides the estimation of the optimum dose of licorice extract to be used in meat products for safety enhancement and storage stability of products. Also, it provide a natural way to increase the stability and safety of meat based products as well as to check food safety concerns but also enhance the protection against spoilage as well as pathogenic bacteria in meat. Availability: Items available for loan: UVAS Library [Call number: 2961-T] (1).
