51.
Influence Of Early Weaning On Growth Performance, Plasma Metabolites And Rumen Fermentation Indices In Neonatal
by Muhammad Afzal Rashid | Prof. Dr. Talat Naseer Pasha | Prof. Dr. Makhdoom Abdul Jabbar.
Material type: ; Format:
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drama
Publisher: 2013Dissertation note: Rearing of young calves is a labor intensive and costly segment of livestock production. From birth to weaning, young calf undergoes a transition from monogastric to adult ruminant. The concept of weaning from milk at an early age is based on early development of functional rumen enabling calves to utilize low quality roughages. In current study, a series of experiments were conducted to refine the more effective weaning regime for buffalo calves and use of by-products of the ethanol production industry in early weaned cattle calves.
Conventionally, buffalo calves are kept with the dam, allowed to suckle a little amount of milk along with seasonal green forages, and weaned around the age of one year. To date, limited published work was available on growth performance and economics of buffalo calves weaned from milk at an early age. Therefore, the experiment was conducted to reduce the weaning age and evaluate the growth performance of male Nili-Ravi buffalo calves. Twenty-four male buffalo calves were assigned to one of the three treatments: continuous milk feeding (CMF), limited milk feeding (LMF), and early weaning (EW). After colostrum feeding, calves were individually fed whole milk at 10% of their BW, adjusted weekly until 6 wk of age. Thereafter, milk allowance was gradually tapered to zero in CMF, LMF and EW treatments at 12, 10 and 8 wk of age, respectively. Calf starter feed was provided ad libitum from wk 2 through wk 12 and individual intakes were recorded daily. Blood sampling was carried out form wk 6 through 12, on a weekly basis. The BW and structural measurements (HG, WH, and HW) were carried out at the start of experiment and later on a weekly basis. In young buffalo calves, the regimen of weaning at 8 weeks of age was more effective. The early weaned calves showed similar growth rate to those in the CMF and LMF by consuming more calf starter and saving a substantial amount of high priced milk. On the basis of the results of this experiment, buffalo calves successfully adapted to early weaning that might help to mitigate issues like poor growth and low returns associated with traditional calf rearing practices. Furthermore, this study effectively reduced the weaning age from 1 year to 8 weeks of age. Hence, reducing weaning age did not affect the growth performance of Nili-Ravi buffalo calves by 12 weeks of age.
Early development of the rumen is the main objective of a successful early weaning program which depends upon the amount of starter intake, VFA production, and ruminal papillae development. Studies have shown that grains in starter feed can be replaced by DDGS up to 28% of DM without compromising the growth performance and rumen development. Second experiment was planned to evaluate the effects of replacing grains and soybean with DDGS and ammonia treated DDGS at 25% of DM. Study was conducted in collaboration with dairy science department SDSU (USA). Twenty one neonatal male Holstein calves were assigned to one of the three of dietary treatments: C = 0% DDGS, DDGS = 25% DDGS, CAFEX-DDGS = 25% CAFEX treated DDGS. In a 10 week experiment, calves were fed 680 g MR through 4 week, reduced to half during wk 5, and weaned at the end of wk 5. Starter intakes were conducted daily; whereas, body weights, structural measurements were conducted at the start of experiment and then on a weekly basis. Jugular blood samples were taken on a weekly basis using EDTA and NaFl coated evacuated tubes. Rumen samples were collected from a subset of 15 calves (n=5 calves/ treatment) at wk 5, 7 and 10. At the end of experiment, four calves from each treatment were also slaughtered to determine rumen morphometric measurements (PL, PW, RWT and PC). Experiment illustrated that weight gain, structural measurements, total starter intake, DMI and feed efficiency were not affected by the inclusion of DDGS and CAFEX treated DDGS at 25% of DM in starter feeds. CAFEX treatment of DDGS improved the CP contents of DDGS from 29.5% to 40%; however, inclusion of CAFEX-DDGS in starter reduced feed intake during the pre-weaning period. Whereas, overall starter intake was higher in calves fed DDGS based starter feed indicating the effect of ammonia treatment on palatability. Lower pre-weaning starter intake, slow rumen fermentation of CAFEX-DDGS resulted in lesser BHBA concentration leading to lesser development of rumen papillae growth (PL and PW). However, there was a tendency for higher weight gain in calves fed DDGS based starter due to increase in starter intake. In the light of these results it is concluded CAFEX-DDGS can be included in starter feeds at 25% of DM without affecting the growth performance. However, further research is required to evaluate the digestibility of DDGS after CAFEX treatement. Similar, growth performance indicates that CAFEX-DDGS can replace the corn and soybean meal in starter feeds.
In third experiment, microbial diversity in developing rumen and intestine of young calves fed DDGS and CAFEX treated DDGS at 25% of starter was investigated. Experiement was carried out at SDSU dairy research station (USA). Fifteen calves with n=5 per treatment, fed according to protocols described in Experiment II. Calves were sampled for rumen contents at wk 5, 7 and 10 of age; whereas, intestinal contents were collected at the time of slaughter. The DNA was extracted subjected to PCR-DGGE and dendogram was constructed using cluster analysis software. Results revealed that microbial population was highly different from each other at wk 10 indicating the effect of age and dietary treatment on rumen micro flora. Whereas, intestinal and rumen bacterial diversity at wk 5 and 7 of age was not affected by inclusion of DDGS and CAFEX-DDGS in starter feed. The changes in intestinal microflora of DDGS and CAFEX-DDGS fed calves compared with control group showed that the effect of dietary treatments on post-ruminal availability of nutrients and microbial proteins. In conclusion, rumen bacterial population changes with the advancing age and the type of ingredients used in the diet. Further, research is required to identify the effect of feeding DDGS on growth of particular bacteria like methanogen and their impact on methane production and feed efficiency.
Availability: Items available for loan: UVAS Library [Call number: 1595,T] (1).
52.
Detection of Insulin-Like Growth Factor-I (IGF-I) Gene Polymorphism in Native Aseel, Desi and Naked Neck Chicken Breeds in Pakistan.
by Asad Ali | Prof. Dr. Khalid Javed | Dr. Afzal Ali | Prof. Dr. Muhammad.
Material type: ; Format:
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drama
Publisher: 2013Dissertation note: Growth traits in chicken production system have an important role. Molecular analysis is an easier mean to identify desirable genotypes for growth. Candidate gene (s) for growth trait like insulin-like growth factor-1 (IGF-1) has imperative function for growth, body composition, metabolic and skeletal traits. The polymorphism of Insulin-like growth factor-1 was detected in native Aseel, Desi and Naked Neck chicken breeds of Pakistan. Fifty, fifty birds of Aseel and Naked Neck breed were selected from Indigenous chicken genetic resource center, Department of Poultry Production, UVAS, Lahore, Ravi Campus. While Desi birds were procured from conventional production farm for genetic analysis. Insulin-like growth factor-1 plays very important role in the cell growth, cell differentiation, food intake of cells and have role in overall body growth, this is why it is being used as a marker to study traits like growth. The polymorphism of IGF-I gene was detected by PCR-RFLP-Pst-Iand this revealed two alleles A (364 and 257 bp), B (621 bp),and three genotypes AA, AB, BB.Genotypic data was analyzed with the help of Pop-gene 1.32 software to calculate genotypic and allelic frequencies. GenotypeAB had the highest frequency in all three native breeds. Genotypic frequency of AA, AB and BB in Aseel was 20, 66 and 14% and in Desi it was 12, 64 and 24 % respectively. While in Naked Neck genotypic frequencies of AA, AB and BB were 18, 60 and 22 %. The highest frequency of allele A (0.53) was found in Aseel while highest frequency of Allele B (0.56) was found in Desi. Genotypic frequency of heterozygotes was highest in all three native breeds i.e. 66, 64 and 60 % in Aseel, Desi and Naked Neck respectively. As the frequency of heterozygotes is significantly higher in all three breeds, so all these native breeds have potential to improve through selection.Furtherstudies are needed to link these polymorphisms with performance traits and then using
that information in future breeding plans for high producing individuals would be very helpful for animal breeder in marker assisted selection (MAS). As Aseel carried highest frequency of allele A so, it can be reared for selective breeding program especially for meat type line development in Pakistan.
Availability: Items available for loan: UVAS Library [Call number: 1637,T] (1).
53.
Phenotypic Characterization Of Two Indigenous Chicken Ecotypes Of Pakistan
by Amjad Iqbal | Dr. Afzal Ali | Prof. Dr | Prof. Dr. Khalid Javed.
Material type: ; Format:
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Publisher: 2013Dissertation note: Poultry is the second largest industry of Pakistan and a significant proportion of poultry products (meat and eggs) originates from the rural poultry production system which is an important source of livelihood for about 80% of the rural households in Pakistan. The quest for greater production through the use of exotic breeds has created difficulties for backyard production systems and is dangerous on economic, social and scientific grounds. This practice is also resulting in rapid erosion of indigenous genetic resources.
The commercial sector itself may require access to indigenous genotypes as its genetic base is quite narrow and for catering the future needs industry may require improvement in many other characteristics (for instance, adaptability and disease resistance) in addition to just egg and meat yield enhancement. Unfortunately, in many countries the indigenous breeds have not been studied for their potential uses and benefits and have been indiscriminately cross bred with exotic breeds. Therefore, it is crucial to study and characterize the indigenous breeds and generate necessary information to be used in future to reap maximum benefits. The generated knowledge would provide useful information essential for conservation of indigenous genetic resources.
The knowledge about the morphological attributes of native chickens has not been adequately documented on technical and scientific grounds. Keeping this in view the present study was planned and data on qualitative and quantitative traits regarding the morphology were collected on Aseel and Naked-neck chickens using a specially designed performa.All recorded data were entered in MS Excel 2010 spreadsheets. The qualitative parameters were expressed as percentages and quantitative parameters were expressed as mean ± standard error.
A great variation in plumage colors and patterns was observed in both Aseel and Naked-neck chickens. The Aseel chickens possessed a pea comb whereas Naked-neck chickens possessed single comb. Also it was interesting to note that about 10% of Aseel hens possessed spurs however spurs were absent in Naked-neck females. On the basis of quantitative traits,it was found that the Naked neck chickens were smaller in size than the Aseel chickens regarding body weight (Aseel: 1938.7±36.9 g in females and 2317±98.4 g in males; Naked neck: 1160.4±19.3 g in females and 1415±22.2 in males), shank length (Aseel: 8.1±0.1 cm in females and 10.3±0.1 cm in males; Naked neck: 7.5±0.05 cm in females and 8.6±0.1 cm in males), shank circumference (Aseel: 44.2±0.5 mm in females and 52.4±1.1 mm in males; Naked neck: 36.5±0.7 mm in females and 41.4±0.5 mm in males), and keel length (Aseel: 15.1±0.1 cm in females and 16.0±0.2 cm in males; Naked-neck: 14.1±0.2 cm in females and 14.7±0.1 cm in males).
The information about indigenous Aseel and Naked-neck chickens documented in the present study can be used as reference in future for conservation and breed improvement programs.
Availability: Items available for loan: UVAS Library [Call number: 1679,T] (1).
54.
Humic Acid Efficacy Study Against Aflatoxin B1 In Broiler Rations
by Rana Yasir Arafat | Dr. Saima | Dr. Abdul | Prof. Dr. Anjum Khalique.
Material type: ; Format:
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Publisher: 2013Dissertation note: Fungus is an important concern in poultry feeds because it can grow at any level of their production right from when grains are in field until the finished product is transported and stored. Therefore it is an immense need to protect raw feedstuff as well as finished feed from the attack of fungus. Aflatoxin is one of the toxins produced by fungi and it causes morbidity and mortality in poultry. In the past different compounds have been employed in order to chelate toxins produced by different types of fungi. Humic acid is produced during the normal decaying process by fermentation of organic materials particularly the plants. Humic acid as a toxin binder is a recent advancement that have got the potential to bind aflatoxins produced by A. flavis and A. Parasiticus. The present study was conducted in order to evaluate efficacy of humic acid to chelate aflatoxin B1.
The study was completed in two phases. In first phase aflatoxin B1 was produced while in second phase the toxin thus produced was employed through feed to commercial broiler chicks. A total of 450 birds were used in five different treatments including positive and negative control. There were 90 birds in each treatment and each treatment was comprised of three replicates of 10 birds in each. In positive control three levels of aflatoxin B1 including 100 ppb, 200 ppb and 300 ppb were added into rations without addition of humic acid. In negative control plain feed was offered without supplementation of humic acid and aflatoxin B1. In rest of three treatments different levels of humic acid including 0.1 percent, 0.2 percent and 0.3 percent were used through feed against three levels of aflatoxin B1 including 100 ppb, 200 ppb and 300 ppb. The parameters including feed intake, body weight gain, feed efficiency, giblet weight, keel length, shank length, dressing percentage, tibial toe ash, gross lesions on carcass, mortality and AFB1 concentrations in serum and liver extracts were studied. The experiment was planned under completely Randomized design with factorial arrangement. The data thus collected on different parameters were subjected to statistical analysis through analysis of variance (ANOVA) technique. The difference among treatment means were compared through Duncan's Multiple Range (DMR) Test at P?0.05.
During first week, birds in all experimental groups showed non-significant difference in terms of feed intake, weight gain and FCR as all the birds in different treatments received plain feed without supplementation of either humic acid or aflatoxin B1. During second week, feed intake, weight gain and FCR were minimum in control group consuming different levels of aflatoxin B1 only, while it was maximum among the birds in group 'B' consuming only plain feed with a non-significant difference in the birds receiving different levels of humic acid. It was observed that with increasing level of humic acid feed intake, weight gain and feed efficiency were linearly improved. Similar pattern was observed in terms of the parameters under discussion during 2nd to 4th week. While during last two weeks of experiment all of the birds in different experimental groups were again put on plain feed without supplementation of aflatoxin or humic acid. During this time period the birds in positive control that had consumed different levels of aflatoxin B1 showed variable levels of improvement in terms of feed intake, weight gain and FCR. Non-significant difference (at P?0.05) was observed among the birds consuming different levels of humic acid as compared to control group consuming plain diet.
Mortalities were maximum in groups 'AD', 'BD' and 'CD' with the highest in 'CD' consuming 300 ppb of AFB1. Dressing percentage was best in 'AK3' and followed to BK2, B, BK1 and CK3. This showed growth stimulant activity of humic acid in addition to anti-AFB1 activity.
Results of giblet weight showed non-significant difference among different groups under study except positive control consuming AFB1. Maximum values were observed in groups 'B' and 'CK3' followed by 'AK2' and 'CK3' while minimum was observed in group 'CD'. Results of heart weight were non-significant among different experimental treatments except 'CD' consuming 300 ppb of AFB1. Results regarding liver weight showed significant difference among the birds in positive control consuming different levels of AFB1. Maximum liver weight was observed in group 'CD' consuming 300 ppb of AFB1. During last two weeks when all of the birds were receiving only plain diet again as withdrawal strategy, the birds in positive control that had consumed purified AFB1 during 2nd to 4th week, showed a reversal effect by exhibiting improvement in giblet weight.
Results obtained regarding ND titres showed maximum titres in group 'AK2', 'B' and 'BK2' while were minimum among the birds in group 'CD' consuming 300 ppb of AFB1. Results were significant with control group consuming different levels of AFB1. The results during last two weeks showed that the birds in different groups consuming different levels of humic acid and particularly in 'CD' that had consumed 300 ppb of AFB1 only during time period of 2nd to 4th weeks showed a reversal effect in developing ND titres. The difference was non-significant (at P?0.05) with control consuming plain diet only.
During the time period of active intoxication of birds with purified AFB1, the results obtained regarding concentration of AFB1 in liver extracts showed non-significant difference among different groups consuming humic acid while significant as compared to control group 'CD' consuming 300 ppb of AFB1. This demonstrated AFB1 chelating activity of humic acid. The results were also significant as compared to group 'B' consuming plain diet only. Maximum concentration was found in group 'CD' consuming 300 ppb of AFB1. After 28th day until 42 days of experiment all of the groups showed a clearance effect of AFB1
Conclusions:
Findings of the current study revealed that addition of increasing levels of humic acid improved the growth and performance of commercial broiler chicks as compared to control group suggesting its potential role in chelating Aflatoxin B1 challenge of different magnitude when given through feed. Therefore, humic acid is recommended for its use as Aflatoxin B1 binder through feed at an inclusion rate of 0.2% of the ration.
Availability: Items available for loan: UVAS Library [Call number: 1749,T] (1).
55.
Effect Of Fibrolytic Enzyme On Digestibility And Production Performance Of Lactating Nili Ravi Buffalos
by Burhan-e-azam | Dr. Saima | Prof | Prof. Dr. Anjum khalique.
Material type: ; Format:
print
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not fiction
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1762,T] (1).
56.
Effect Of Mannan Oligosaccharides (Mos) From Saccharomyces Cerevisiae Alone And In Combination With Antibiotic
by Musawir Rehman | Prof. Dr. Anjum Khaliqe | Dr. Saima | Prof. Dr. Muhammad.
Material type: ; Format:
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Publisher: 2013Dissertation note: Poultry feed industry worldwide has been using antibiotics for more than 50 years. But the use of antibiotics as growth promoters was completely banned in 1999 by the European Union (EU) (European Commission, 2001). This was due to increases in microbial resistance to antibiotics and residues in chicken meat products which might be harmful to consumers.
In Pakistan poultry feed industry is using antibiotics. But internationally their alternatives mannan oligosaccharides (MOSs) are being used. MOSs, which are derived from the cell wall of Saccharomyces cerevisiae, have shown promising effects, such as decreasing pathogenic microflora of the gut, stimulating a strong immune response, and elevating the strength of the intestinal mucosa in poultry. Exact mechanisms by which MOSs show positive effects are not known but some suggestions are they provide nutrients, they aid in digestion of foods and inhibit harmful bacteria in the gut (Owings et al.1990). Gastrointestinal normal flora plays an important role in the health and performance of poultry (Thong song et al.2008). It is therefore of vital importance, both from an animal and human health perspective, to obtain natural alternatives like mannan oligosaccharide (MOSs).
MOSs have been shown to increase growth in broilers (Yusrizalet al. 2003), turkeys (Kannanet al.2005), and results of many trials indicated that MOSs can be one of the best alternatives to antibiotic growth promoters (Hoogeet al.2003), and even trace amounts of MOSs added to a ration were quite effective in increasing the health status and production of poultry (Savage et al. 1997). Stanczuk et al., reported that supplementation of rations with MOSs and inulin (0.1% and 0.4% MOS in diet) did not significantly affect live body weight (BW), feed intake (FI), or feed conversion efficiency (FCE) in turkeys in an 8-week trial (Stanczuket al., 2005).
Actigen a product by Alltech, Inc., is having advanced form of mannan oligosaccharide, which was offered in feed to broiler birds in this trial. It acted as alternative to antibiotics without affecting the performance of birds, It protected the birds from pathogens. Keeping in view the situations the present project was planned. In this studymannan oligosaccharide (MOS) was evaluated on broiler (5*6*15=450) as antibacterial and growth promoter alone or in combination with antibiotic (neomycin) in environmentally control shed of Poultry Research and Training Centre, Department of Poultry Production, UVAS-Ravi Campus, Pattoki. Duration of this trial was 35days.The parameters of this experiment were feed intake, body weight gain, feed conversion efficiency, mortality, carcass characteristics, and slaughter parameters, estimation of ALT, AST and cholesterol from blood. Statistically data was analyzed by using ANOVA in completely randomized design (CRD) with help of a special computer based statistical package (SAS9.1, 2002-03).The results showed that there was significant difference in feed intake when birds were fed with 0.1% MOS with antibiotic from other dietary treatments. MOSs and antibiotics alone or in combination depicted significant results upon weight gain. They were significantly different from control. There was significant decrease in FCE in groups where MOSs was used in combination with antibiotic and more in 'A' 'B' and 'D' respectively. There was significant decrease numerically regarding mortality but statistically there difference was non-significant. Effect of dietary treatments on relative organs weight was non-significant. There was also a non-significant effect on dressing %age. Regarding sera ALT level there was significant decrease in treatment groups than control group. Value of AST level was also significantly less in treatment groups than control group. There was not a significant difference in serum values of triglyceride. Cholesterol level decreased in treatment groups 'B' and 'C' significantly than control but increased significantly in groups 'D' and 'E' than control group. The outcome of this study is that MOS have definite effect on bird's performance and could be used in combination or as an alternative to antibiotics. It is cost effective and beneficial for human health and poultry industry of Pakistan.
Availability: Items available for loan: UVAS Library [Call number: 1765,T] (1).
57.
Fiber Levels Durig Different Physiological Stages In Nili Ravi Buffaloes
by Saeed Ahmed | Prof. Dr. Makhdoom Abdul Jabbar | Prof. Dr. Anjum Khalique | Prof. Dr. Khalid.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1779,T] (1).
58.
Mineral Profile In Ruminants Of Canal Lrrigated Districts Of Punjab And The Effect Of Dietary Cation Anion Diffference on Lactational Performance of Nili Ravi Buffaloes and Beetal Goats
by Umar farooq | Prof. Dr. Talat naseer pasha | Prof. Dr. Makhdoom abdul jabbar.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1855,T] (1).
59.
Efficacy Of Toxin Binder In Reducing Induced Aflatoxin B 1 And Ochratoxin A In Broiler Chickens
by Muhammad Umer Zahid | Prof. Dr Anjum khalique | Dr. Saima | Mr. Jibran hussain.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1939,T] (1).
60.
Genetic And Phenotypic Evaluation Of Sheep And Goat Flocks Maintained At Small Ruminant Training And Research Center UVAS
by Raheela saeed malik | Prof. Dr. Khalid javed | Dr.Afzal ali | Mr. Qamar shahid.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1950,T] (1).
61.
Comparative Study Of Different Levels Of Neutral Detergent Fiber In Sahiwal Cattle And Nili-Ravi Buffalo Heifers
by Usama Tayyab | Prof. Dr. Anjum Khalique | Prof. Dr. Talat Naseer Pasha | Prof.Dr.Khalid.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1979,T] (1).
62.
Slow- Release Non-Protien Nitrogen Impact On Performance Of Weaned Crossbred Calves
by Shahzad naveed | Prof. Dr. Talat naseer pasha | Prof. Dr | Prof. Dr. Anjum khalique.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2030,T] (1).
63.
Effect Of Supplementation Of Choline And Betaine On Performance And Blood Triglyceride Level Of Broiler Chicks Fed Methionine Level
by Nadeem akhter | Dr. Saima | Prof. Dr. Anjum khalique | Prof. Dr. Athar.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2040,T] (1).
64.
Isolation Of Phytase Gene From Bacteria Obtained From Different Sources
by Raja Danish munir | Prof. Dr. Masroor ellahi babar | Prof. Dr | Prof. Dr. Khalid javed.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2065,T] (1).
65.
Slow-Release Non -Protien Nitrogen Impact On Performance Of Weaned Crossbred Calves
by Shahzad naveed | Prof. Dr. Talat naseer pasha | Prof. Dr.Anjum khalique.
Material type: ; Format:
print
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2071,T] (1).
66.
Performance Evaluation Of Nili Ravi Buffalo Calves In Response To Wholemilk Vs Milk Replacer
by Muhammad Atif saeed | Prof. Dr. Talat naseer pasha | Dr. Muhammad Naveed ul Haque.
Material type: ; Format:
print
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2072,T] (1).
67.
Efficy Of Symthetic Antioxidant In Stabilizing The Various Levels Of Poultey By Product Meal In Broiler Diet For Subsequent Growth Performance
by Haseeb Ahmad | Prof. Dr Anjum Khalique | Dr. Saima.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2096,T] (1).
68.
Cumulative Effect Of Phytase And Vitamin D On Broiler Performance
by Muhammad Mehran | DR. Saeed Ahmad | Prof. Dr. Anjum Khalique | Prof. Dr. Khalid.
Material type: ; Format:
print
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2121,T] (1).
69.
Association Study Of Leptin Gene With Growth Trait In Lohi Sheep
by Ali Haider Saleem | Prof. Dr. Khalid Javed | Prof. Dr. Masroor Ellahi Babar.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2132,T] (1).
70.
Effect Of Diets Formulated With Different Nutrient Densities On Performance And Blood Parameters Of Broilers At Different Phases.
by Hafiz Gulfraz Ahmad (2007-VA-089) | Prof. Dr. TalatNaseer Pasha | Prof. Dr. Saima | Prof. Dr. Athar Mahmud.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: Poultry sector, the second largest industry of Pakistan, is growing exponentially to fulfill the growing needs of meat and eggs. Although poultry sector has shown incredible growth rate during the past decade; however, there is still gap between poultry meat demand and supply. Every living animal has a need for protein. It is the basic structural material from which all body tissues are formed. Dietary protein and metabolize-able energy content has re-emerged as a topic of interest and importance over the last few years mainly due to environmental concerns. Availability of balanced and economical ration has great importance in broiler production. Feed cost is the single most important factor, which accounts more than 70 percent of total cost in poultry production. The challenge for nutritionist and production managers is not only to provide a feed cheaper to a maximum possible level, but also to maximize profitability, because performance of the bird depends upon a broad range of dietary nutrients.
Two hundred day old chicks were purchased from the commercial hatchery. On the first day all chicks were weighed and were separated into 4 dietary treatment groups in completely randomized design so that each experimental treatment consist of 5 replicates having 10 chicks in each replicates in this way each treatment consist of total 50 chicks(4x5x10=200). Feeder and waterer were clean before start of experiment and were cleaned regularly till the end of experiment. Feed and water which were provided throughout experiment were clean, Ad libitum and completely hygenized.
Four experimental diets were prepared according to the Aviagen Recommendation (2012) but the level of ME in all groups were different, HD feed with ME (3000-3200 Kcal/Kg) and CP (19-24 %), MD feed with ME (2900-3100 Kcal/Kg) and CP (19-23), ND feed with ME (2800- 3000 Kcal/Kg) and CP (19-23 %) and LD feed with ME (2700-2900 Kcal/Kg) and CP (18-23 %) respectively. Data were collected for different parameters of growth performance, carcass traits and blood TG and HDL in mg/dl, respectively. The economics of each treatment was analyzed at the end data was analyzed through Analysis of Variance (ANOVA) with use of Completely Randomized Design.
The highest weight gain (1862.4± 106.56) was observed in group HD having (ME: 3000 Kcal/Kg in Starter ration, 3100 Kcal/Kg in grower ration and 3200 Kcal/Kg in finisher ration) and lowest (2974.6±26.96) was observed in group LD having (ME: 2700 Kcal/Kg in Starter ration, 2800 Kcal/Kg in grower ration and 2900 Kcal/Kg in finisher ration) at 35 days of age. Overall result showed that there was significantly decline in weight gain with decreasing level of ME in diet .Statistical analysis showed that all treatments in starter and finisher rations were different (P≤0.05) except in finisher ration.
Carcass traits were non-significant (p > 0.05) to each other throughout experiment. Dressing percentage of broilers of different treatment groups was highest (60.38±7.90) in MD group and lowest was observed in LD feed group (54.6±10.37). Dressing percentage was increased as the level of metabolize-able energy increased in diets.
The highest blood Triglyceride level (36.3±2.5) was observed in HD group and lowest (29.5±3.92) was observed in ND group. Statistical analysis showed that there was a strong significant difference (P≤0.05) among all these treatment. Same results were found in case of HDL levels in blood. Data analysis proved that MD feed was most economical with good results.
Availability: Items available for loan: UVAS Library [Call number: 2308-T] (1).
71.
Efficiency Of Nitrogen Utilization In Response To Level Of Crude Protein Supplies In Primiparous Lactating Nili-Ravi Buffaloes
by Saba Anwar (2006-VA-68) | Dr. Muhammad Naveed ul Haque | Dr. Fayyaz Ahmed | Prof. Dr. Anjum Khalique | Dr. Muhammad Qamar Shahid.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: Background:
In lactating buffaloes the protein requirements essentially depend on milk production & milk protein content together with the efficiency of conversion of dietary nitrogen to milk nitrogen. Lactating buffaloes utilize crude protein efficiently but still excrete some nitrogen (N) in feces. This inefficient N conversion results in increased feed cost and environmental pollution. There is need for the estimation of optimum level of protein required for lactating buffaloes for its maximum utilization and minimum wastage.
Hypothesis:
To check the efficiency of 3 different levels of crude protein this is better utilized for milk production of buffaloes.
Methodology:
The experiment was carried out at BRI, Pattoki. Six (6) Nili Ravi primiparous buffaloes in mid lactation were used for this experiment. Animals received 3 treatments in a 3×3 latin square design with a 21 days (d) period (total duration of experiment will be 63d). The treatments contain diets are 9% (CP9), 11% (CP11), and 13% (CP13) of crude protein content. Diet distributed as well as orts (refused) was noted on daily basis. Fodder samples were collected after every week in each period for evaluating the DM and for proximate analysis. Concentrate and wheat straw was analyzed once in each period. Milk yield of buffaloes were recorded daily and milk samples were collected for determining composition of milk. Body weights were taken on fortnightly basis. Blood samples were collected for analysis of different physiological metabolites.
Statistical Analyses:
Data was analyzed through General Linear Model by using PROC GLM command (SAS, 2004). Further polynomial linear and quadratic regression were used to determine the effects.
Outcome:
It was concluded from the study that high protein intake in the diet did not increased the DMI and milk parameters except fat contents. Level of BUN increased at high CP diet which depicts the inefficiency of nitrogen utilization by buffaloes. Buffaloes are less efficient in nitrogen utilization than cows. The low protein treatment appears sufficient to support milk production of 9 kg/d. Availability: Items available for loan: UVAS Library [Call number: 2453-T] (1).
72.
Effect of two Feeding Regimens of Sodium Humate on Health and Production Traits of Lactating Nili-Ravi Buffaloes
by Shaheen Iqbal (2004-VA-16) | Prof. Dr. Saima | Prof. dr. Anjum Khalique | Prof. Dr. M. Abdullah.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Thesis submitted with blank cd. Availability: Items available for loan: UVAS Library [Call number: 2472-T] (1).
73.
Morphological Structure Of Thalli Sheep Through Principal Component Analysis Of Body Measurements Muhammad
by Muhammad Arslan Akbar (2014-VA-07) | Prof. Dr. Khalid Javed | Dr. Afzal Ali | Dr. Nisar Ahmad.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Mutton is also very extensively used food and sources of mutton are only sheep and goat.
Sheep have a great genetic potential to fulfill the increasing demand of mutton in our country.
Body conformation and features are very important traits in milch, meat and wool animals. In
developing countries, record keeping is at initial level and the records about pedigree and progeny
of individuals are insufficient and do not provide the estimation about genetic parameters.
Therefore, phenotypic information are necessary for the explanation of relationship among linear
type traits and selection is based on these traits. Principal component analysis technique has been
used to identify the body size, body shape, head size and over all body conformation in Zulu Sheep.
Animal conformation and genetic parameters can be measured by using the technique of
phenotypic characterization.
Data on morphometric traits of Thalli sheep were collected from “Small Ruminant
Research and Development Centre, Rakh Khairewala, District Layyah, Punjab, Pakistan” and
Livestock Experiment Station, Rakh Ghulaman, District Bhakkar, Punjab, Pakistan. Different
phenotypic parameters and twenty one (21) morphometric traits were measured on animals of
Thalli sheep. The traits measured were birth weight, body weight, heart girth, body length, withers
height, head length, head width, ear length, ear width, neck length, neck width, barrel depth, sacral
pelvic width, rump length, rump width, tail length, testes length, testes width, scrotal diameter, teat
length and teat diameter. Different phenotypic characters was recorded as body color, body shape,
eye color, head color, fore head color, face color, face structure, chin color, ear color, ear nature,
appendages color, nostril structure, muzzle structure, neck structure, tail color and tail switch.
Summary
174
Weighing balance (digital) was used for determination of body weight and a flexible
measuring tape (tailor tape) was used to record the different body measurements. To avoid
variations among individuals, measurements were taken by the same person. Animals of different
age groups were reared at these research stations. Animals were divided into different groups (A,
B, C, D, E, F, G, H and I) according to their age as 0-3, 4-6, 7-9, 10-12, 13-15, 16-18, 19-21, 22-
24 and above 24 months. Each group was further divided into two sub-groups of males and females
animals. Normality of data were checked against all animals (overall group and separate groups)
and all animals were fallen in ±3SD but two outliers had been removed. Data on morphometric
traits were analyzed statistically for mean, range, coefficient of variation and standard error.
Pearson’s coefficient of correlation among different biometric traits was estimated and data were
generated for principal component analysis (PCA) from the correlation matrix. Regression
equations were developed for the estimation of body weight.
Descriptive statistics (mean, range, standard deviation and coefficient of variation) of body
measurements of overall female were showed coefficient of variations of overall female animals
of Thalli sheep for mostly variables were ranged from 10-20% and coefficient of variations of birth
weight and body weight were 22.38% and 25.75% respectively. Coefficient of variations of male
animals of Thalli sheep for linear body measurements were ranged 08-25% and tail length had
high coefficient of variations as 26.89%. Male animals of all age groups are heavier than females.
Correlation coefficients of morphometric traits of overall females and males of Thalli sheep
were highly positive and significant (P≤0.01) among withers height, body length, heart girth, head
length, head width, ear length, neck length, neck width, rump length, rump width, barrel depth,
sacral pelvic width and body weight. Withers height, heart girth and body length were observed to
be significantly correlated with each other as well as with live body weight of all age groups.
Summary
175
For overall female animals, two principal components were extracted with eigenvalues
9.005 and 1.558 and 56.279% and 9.740% variances for PC1 and PC2 respectively and their
cumulative variance was 66.020%. For overall male animals, three principal components were
extracted with eigenvalues greater than 1 and PC1 showed high variance 57.516% and PC2 and
PC3 had variances as 12.184% and 7.022% respectively and their cumulative was components
76.721%. In all age groups which has been studied, withers height, body length and heart girth
have high values in commonalities as well as in component matrix. PC1, PC2 and PC3 showed
maximum variations in almost all age group studied.
Regression equations developed to estimate of live weight of all age groups were indicated
that almost all equations had variables withers height, body length and heart girth.
Conclusion:
From findings of present study, it was concluded that body measurements (Withers height,
body length and heart girth) had high correlations with each other and with body weight in almost
all age groups. Principal component analysis of morphometric traits was showed that most of
variation explained by PC1 and in some groups, PC2 and PC3 had also more effects.
Commonalities were higher which showed that all the variables were important but PC1 had high
values for withers height, body length and heart girth and maximum variance. This indicated that
morphometric traits are very important for selection of genetically elite animals. Morphometric
traits can be used to estimate the body weight in the field conditions, where weighing balance is
not usually available. However, further research is needed to investigate the relationship among
different morphometric traits in other breeds of goats, sheep and other livestock breeds like cattle,
buffalo, camel and horse of the country. Availability: Items available for loan: UVAS Library [Call number: 2471-T] (1).
74.
Effect Of Different Sources Of Selenium On Egg Production, Egg Quality And Egg Selenium Concentration In 2ND Phase Of Laying
by Farhat Abbas 92013-VA-891) | Prof. Dr. Saima | Prof. Dr. Anjum Khalique | Dr. Jibran Hussain.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Se is an essential element required for the human body. On the daily basis food consumed by human does not fulfil the requirement of Se for the body. Se cannot be directly consumed as it is harmful for the human body that’s why scientist are trying to produce Se enriched foods for human consumption. In this way eggs are the best medium through which consumable Se can be provided to the human by fortifying eggs when Se given to the birds in feed.
Different sources of Se supplementation in the diet may affect the egg shell quality, albumin height and selenium concentration in the eggs when given to ISA hi-sex white commercial layer.A total of 240 birds from ISA hi-sex fed with two Se sources and having three levels (0.2, 0.4, 0.6 mg/kg) each i.e. organic Selenium Yeast (SY), inorganic Se as Sodium Selenite (SS) and combination of organic and inorganic sources of selenium (SY+SS) one control were used in present study. Daily egg production, egg weight and weekly feed efficiency was recorded. Egg shell thickness, egg shell weight, albumen height, yolk index and haugh unit were measured every week. Whole egg Se concentration was determined 2nd, 4th and 8th week of the experiment.
Data collected were analyzed through completely randomized design (CRD) under one ANOVA technique (Steel et al. 1997). Means were separated through Duncan’s Multiple Range test (Duncan’s, 1955) with the help of SAS 9.1. The results of the observed study showed that supplementation of organic Se in the layer hen’s diet improved the albumen height (mm), Haugh unit score and yolk index. In this experiment different sources and levels of Se did not show the statistically significant difference in the results of egg production, egg weight, egg shell weight and egg shell thickness. All the sources and levels of Se improved the whole egg Se concentration and the organic Se showed much better results in whole egg Se concentration as compared to other sources of Se. Availability: Items available for loan: UVAS Library [Call number: 2574-T] (1).
75.
Impact Of Digestible Lysine, Methionine + Cysteine Ratios On Growth Performance, Ileal Digestibility Of Protein And Gut Health In Broilers Fed Different Protein Sources
by Ch. Ahsan Akram (2009-VA-397) | Prof. Dr. Talat Naseer Pasha | Dr. Shafqat Nawaz Qaisrani | Prof. Dr. Athar Mahmud.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Poultry production is a vibrant segment of agriculture in Pakistan. Genetic selection and nutritional improvements resulted in accelerated growth rate and better FCR in modern broilers. This improved broiler performance requires greater amount of digestible nutrients. Since feeding cost accounts about 70% of total production cost and protein is the most expensive component of poultry diets. Future poultry diets, therefore, will be formulated by using cheaper and poorly digestible protein sources such as CM and SFM due to price volatility of highly digestible but expensive sources like SBM. The undigested protein in hind gut is subjected to microbial fermentation, results in formation of nitrogenous products that are not only harmful to birds, but also excreted into litter bedding and generate ammonia, nitrous acid and nitrous oxide, the latter one is a powerful greenhouse gas with an effect about 300 times compared with carbon dioxide. The potential solution to this problem is supplementation of synthetic amino acids in low nutrient density (CP) diets formulated by using cheaper and poorly digestible protein sources. Therefor a study was designed with the objectives to determine the impact of dLys, M + C ratios on growth performance, apparent Ileal digestibility of protein and gut health in broilers fed poorly digestible protein sources (CM and SFM). An experiment with 432 mixed sex (Ross 308) 1-d-old broilers was conducted to test the hypothesis that supplementation of Meth may improves growth performance, apparent Ileal digestibility of protein and gut health in broilers fed poorly digestible protein sources (CM and SFM). The interaction effects of the protein source (CM vs. SFM), CP levels (high vs. low) and dM+C/d Lys (recommended vs. supplemented) were tested in a factorial arrangement of 8 (2 × 2 × 2) dietary treatments. The supplementation of Meth positively influenced FI (P = 0.042), BWG (P = 0.003) and FCR (P <0.001). The broilers fed Meth supplemented diets had, on an average, 10% greater apparent Ileal digestibility of protein, 34% longer villi, 24.5% shorter crypts and 60% greater VCR compared with those fed diets with recommended levels of Meth. The levels of CP, similarly, influenced (P <0.001) the FI, BWG and FCR. The broilers fed high CP diets had 9% improved apparent Ileal digestibility of protein compared with those fed low CP diets. In conclusion, supplementation of Meth improves the broiler growth performance, apparent Ileal digestibility of protein and gut health fed poorly digestible protein sources (CM and SFM). The negative effects of poorly digestible protein sources thus may be partially ameliorated by supplementation of Meth.
CONCLUSIONS
In conclusion, the negative effects of poorly digestible protein sources thus may be ameliorated by supplementation of Meth. Supplementation of Meth above the recommended levels improves the growth performance, Ileal digestibility of protein and gut health in broilers fed poorly digestible protein sources (CM and SFM).
SUGGESTION AND RECOMMENDATION
Further research is needed to evaluate the different strategies to improve the digestibility of poorly digestible protein sources including guar meal. Threonine also has an important role in gut health so there is a need to study the effects of supplementation of threonine above the recommended levels in broilers fed poorly digestible protein sources. Feed form has pronounced effects on gut development, digestibility of nutrients and subsequent growth performance of birds. There is a dire need to evaluate the other cheaper protein sources, supplemented with synthetic amino acids and to find out a possible interaction between feed form and poorly digestible protein sources that may replace SBM in broilers.
Availability: Items available for loan: UVAS Library [Call number: 2567-T] (1).
76.
Effect of Super Doses of Phytase from Two Different Sources on Growth Performance, Phosphorus Utilization and Carcass Characteristics of Broilers
by Faisal Mushtaq (2009-VA-369) | Prof. Dr. Saima | Prof. Dr. Athar Mahmud.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Cereal grains contain almost two-third of the P that is present in phytate form. Phytate possesses anti-nutritive properties as it binds other nutrients like amino acids and carbohydrates. Birds are unable to use P bound with phytate complex. The phytate P is excreted into the environment causing pollution. To compensate requirements expensive inorganic P sources like DCP and rock phosphate are used in poultry diets, which ultimately increase the ration cost. Exogenous phytase is capable of degrading phytate into lower esters having less chelating capacity. Commercially phytase enzymes are being added in the poultry diets to hydrolyze the phytate P that can replace the expensive inorganic P addition. In the industry, phytase is being added in the poultry diets at 500 FTU/kg. Use of phytase at this rate still leaves some gap. Keeping this thing in view present study was planned to use the super dose of phytase from two different sources (Buttiauxella and E.coli) to minimize the anti-nutritive aspects of phytate and use the phytate P at maximum. For this purpose 35 day feeding trial was conducted to evaluate the effect of super dosing of phytate from two different origins on growth performance, phosphorus utilization and carcass characteristics of broilers. For this in total, 672 one-day old broiler chicks were randomly selected& assigned to 8 dietary treatments, each having 3 replicates of 28 birds. One group was reared on positive control diet formulated according to the Hubbard strain recommended guides with adequate P without any phytase addition. Second group was offered NC diet (deficient in Av.P). While other six diets were formulated with three levels (500, 1000, 1500 FTU/kg) of E.coli and Buttiauxella origin supplemented in NC diet. The FI, BWG and FCR were recorded at the end of each week. At the end of experiment n=3 birds per replicate were selected randomly, weighed and slaughtered to estimate plasma P, ileal digestibility of P, tibia ash, organ weight and dressing percentage. The data collected were analyzed using analysis of variance (ANOVA)
SUMMARY
41
technique in a Completely Randomized Design (CRD) and means were be compared through Duncan’s multiple range test (DMR) (Duncan 1955) by using SAS (version 9.1; SAS Inst. Inc., Cary, NC).
Results of the experiment revealed that FI, BWG and FCR was higher 48%, 11% and 64.5% respectively in phytase supplemented diet best treatment T8, when compared to the NC. Phytase supplementation had no effect on organ weights. Organ weight was higher in diet low in NPP. Phytase supplementation had no effect on gizzard weight even at higher doses. Similarly phytase supplementation had positive effects on bone mineralization. Phytase addition in the diets improved tibia ash, Ca and P% at all levels (P <0.01). Plasma P level was significantly increased at higher level of phytase. The AIDC of P was linearly increased by phytase supplementation. Considering the economics phytase supplemented diets at higher level 1500 FTU/kg were most economical when cost of feed calculated to attain 1kg of live weight.
From this experiment it can be concluded that phytase supplementation at higher dose than the standard ensures the maximum break down of phytate P and reduce its anti-nutritive aspects apart from the source of phytase used in the experiment. Dietary inclusion of phytase at super dose economically generates the bio-available P and reduces the P load in the environment improve growth performance, and bone mineralization.
To replace the highly expensive phosphorus sources super dose of phytase can be used instead of standard dose. It can replace the expensive source of inorganic P and economically can generate more profit. Optimum level of phytase in the diet need to be reevaluated to replace inorganic P to some extent, minimize the anti-nutritive aspects of phytate and reduce P load in the environment. Availability: Items available for loan: UVAS Library [Call number: 2607-T] (1).
