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1. Comparative Efficiency Of Routine Identification Methods With Molecular Technique (Pcr) For Detection Of Caecal Eimeria Species in Broilers

by Muhammad Yasir | Prof. Dr. Kamran Ashraf | Dr. Aftab | Prof. Dr. Azhar Maqbool.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2011Dissertation note: Caecl coccidiosis is caused by Eimeria tenella and E. necatrix and poultry industry is facing huge economic losses due to this infection world wide. Eimeria tenella is the most common cause of caecal coccidiosis but E. necatrix is also isolated rarely. In the present study, 400 caecal samples suspected for coccidiosis were collected from two districts (Lahore and Gujranwala). Ten samples from twenty farms of each district were collected and examined for coccidiosis and species identication. Prevalence of coccidiosis was found 68%. It was more prevalent in district Gujranwala (71.5%) as compared to Lahore (64.5%). Eimeria species were identified by conventional (Direct Microscopy, Sedimentation technique, Floatation Technique and Sporulation) and molecular technique (PCR). Polymerase chain reaction was found the most sensitive ands accurate technique for species identification as compared to all conventional techniques. The prevalence percentage of E.tenella and E,necatrix was found 68% and 12.5% respectively. E.tenella was more prevalent in district Gujranwala (71.5%) as compared to district Lahore (64.5%). E.necatrix was also more prevalent in district Gujranwala (13.5%) than district Lahore (11.5%). The difference in percent prevalence of coccidiosis and species of Eimeria may be due to difference in farm management, farming type, biosecurity measures. Availability: Items available for loan: UVAS Library [Call number: 1274,T] (1).

2. Mosquitocidal Efficacy Of Entomopathogenic Fungus Metarhizium Anisopliae And Its Combined Therapy

by Aalia Riaz | Prof. Dr. Kamran Ashraf | Prof. Dr. Azhar Maqbool | Prof. Dr. Tahir.

Material type: book Book; Format: print Publisher: 2012Dissertation note: The aim of study was to find out the method for disposal of waste material, dead birds and poultry litter and their proper utilization in the poultry industry. Secondly to evaluate the efficacy of composted poultry litter/dead birds in broiler quail ration. The experiment was conducted at Poultry Research and Training Centre and Avian Research and Training Center, UVAS, Lahore in two different phases. The first phase was of 8 weeks duration in which composting of dead birds was doneusing advance windrow pile technique and proximate analysis of the composted material wascarried out. During the second phase, a quail ration was formulated according to dietary recommendations of NRC (1994) with inclusion of 0, 2, 4 and 6% compost and fed to quails,For this purpose, a total of 1200 day old Japanese broiler quails were randomly divided into 4 different experimental groups (A, B, C, and D). Group A was control and group B, C, and D contained 2, 4, and 6% composted ration respectively. The birds in each group were replicated six timescomprising 50 birds in each replicate. After 4 weeks of age three birds per replicate were slaughtered and their slaughtering parameters were recorded. The data thus obtained were analyzed through ANOVA in completely randomized design (Steelet al.1997) and means were compared by Duncan's Multiple Range (DMR) test (Duncan, 1955) using SAS (Statistical Analysis System) version 9.1. In production performance feed intake, body weight, body weight gain and FCR showed positive response when fed different levels of composted diet while mortality % remained unaffected throughout the experimental period. In slaughtering parameters live body weight (g), carcass weight %, dressing Weight %, Giblet weight %, Gizzard weight % and Heart weight % showed positively when fed different levels of composted diet while liver weight % remained unaffected throughout the experimental period. Key Words: Composted ration, Japanese quail, Production Performance, Slaughtering Parameters. Availability: Items available for loan: UVAS Library [Call number: 1489,T] (1).

3. Prevalance Of Endoparasites In Common Carnivorous Fish In Lahore.

by Imtiaz Aziz | Prof. Dr. Azhar Maqbool | Prof. Dr | Prof. Dr. Kamran Ashraf.

Material type: book Book; Format: print Publisher: 2012Dissertation note: Due to intensification of fish culture practices, there is more likely the occurrence of various diseases in general and parasitic in specific in fish. However, there is a paucity of information regarding the prevalence of various parasites in local fish especially fishes of fresh water. Therefore, the present study has been planned to investigate the prevalence of various parasites of the gastrointestinal tract and liver of Wallago attu(Malee) ,Rita rita (Khaga), Channa marulius(Saul) and Mystus seenghala(Seenghari). Therefore, it was hypothesized that there is a variation in the occurrence of various endoparasites in various species of fish. A total fifty gut samples from each of breed of fish (Wallago attu (Malee),Rita rita (Khaga), Channa marulius(Saul) and Mystus seenghala(Seenghari) were collected from various fish markets located in Lahore City like Bhatti Gate, Samnabad, Tollinton Market, and were analyzed to determine the prevalence of various endoparasites in the intestine. The gut samples were collected in 0.9% physiological saline and were preserved in 4% formalin. The recovered parasites were fixed and identified on the basis of morphology to calculate the prevalence rate. The data thus collected was analyzed using a Pearson's chi-square. Results showed that out of 65 fish samples collected from Tollinton market that included Rita rita (n = 20), Wallago attu (n = 15), Channa marulius (n = 15) and Mystus seenghala (n = 15), 7 (10.76%) samples of gut harbored various parasites. Similarly, the prevalence rate of parasites in two other localities were 17.39% in Bhatti-gate fish market and 18.18% in Samanabad Fish market. The overall prevalence rate was found to be 15.5%. There was no statistical difference in the prevalence rate when data was assorted on the basis of localities. Both single and mixed infections were noted in fishes collected from all three localities and all four species of fresh water fish. The various parasites revocered from the intestines of the gut from all species included P. heteropneustus Cucullanus sp., Cucullanus sp., Phyllodistomum sp. and R. magna. Availability: Items available for loan: UVAS Library [Call number: 1499,T] (1).

4. Epidemiology, Zoonotic Potential, Haematiology And Therapy Of Toxocariasi In Dogs And Humans.

by Nisar Ahmad | Prof. Dr. Azhar Maqbool | Prof. Dr | Prof. Dr. Kamran Ashraf.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2010Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1505,T] (1).

5. Prevalence And Chemotherapy Of Trichostrongyloids In Camels In Charsada

by Muhammad Qasim | Prof. Dr. Azhar Maqbool | Dr. Muhammad | Prof. Dr. Kamran Ashraf.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2131,T] (1).

6. Epidemiology Zoonotic Potential Haematology Amd Chemotherapy Of Sarcoptic Mange In Camel In Punjab

by Muhammad Irfan Zahid (2011-VA-800 | Prof. Dr. Azhar Maqbool | Prof. Dr. Muhammad Sarwar Khan | Prof. Dr.Shazia Anjum | Prof. Dr. Kamran Ashraf.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2015Dissertation note: A camel is a very hardy ruminant animal, which can survive under harsh climatic conditions very effectively by utilizing the marginal areas with excellent capabilities and produce under such conditions (Hjort and Hussein, 1986; Abbas and Tilley, 1990). Camel is an important animal as it is well adopted in unique manners in the hot, arid and semi-arid environments (Schwartz, 1992). It can survive without water and food for many days and this unique ability of camel makes it an ideal for such harsh conditions for which it is also commonly known as “The Desert Ship”. In spite of the fact that camel is an important member of a group of animals which produces food for human consumption in the shape of milk and meat, yet it is the most neglected one in the field of scientific research. It may be due to the fact that camel belongs to such areas of the world which are arid, semi-arid or rain fed in nature, having harsh climatic conditions, where poor nutrition and poor management are the major issues (Sohail, 1983). It is an established fact that diseases originating from parasites lead to the main health hazard issues in animals. These parasites survive at the expense of the host animals causing lot of health problems, like skin irritation, anemia leading to weakness and debility. Some of the parasites have zoonotic importance and may become a source for the transfer of many contagious diseases like scabies to the human beings (Dominguez et al. 1978). McClain et al. 2009, observed the scabies as a major health problem globally both for humans and animal population. Sarcoptes scabiei is an ectoparasite which is a cause of scabies, a skin problem in the human beings worldwide and the similar species of mites do also produce a similar type of disease in a large variety of wild and domesticated mammals (Pence and Ueckermann, 2002; Fitzgerald et al. 2004). Fain, 1978, reported that more than fifteen (15) different species of Sarcoptes scabiei morphologically and genetically distinct from each other have been identified in different hosts. Introduction 2 Sarcoptic mange is the second important problematic disease of camel after Trypanosomiasis (Nayel and Abu-Samra, 1986). Scabies caused by Sarcoptes scabiei var cameli is a serious & highly contagious skin problem and also economically important disease of the camels (Pegram and Higgins, 1992). Camels, which are reared with deficient nutrition, poor management and under unhygienic conditions are mostly affected by this disease (Kumar et al. 1992). A large group of people and communities living in arid diverse ecozones in the entire world, particularly in harsh climates earns their livelihoods by depending on camels. This dependence may spread to the utilization of camel milk, meat, wool and leather besides its use in transportation, riding and sports (Wilson, 1984; Snow et al. 1992). In Pakistan camels are also raised by the people for meat, milk, riding, transportation and sports purposes in the deserts, semi desert & rain-fed / warm areas of the entire country being a hardy animal as it can tolerate easily the rugged climate as well as extremes of temperatures of such areas. The natural harsh and adverse climatic conditions, particularly during long dry seasons lead to a paucity of feeding regimes resultantly the camels raised in such areas are subjected to stress conditions which lower their resistance and make them easily vulnerable to diseases (Abbas et al. 1993; Agab, 1993). Abbas & Tilley, 1990; Saint-Martin et al. 1992; Abbas and Agab, 2002; Pathak and Chhabra, 2010; while reviewing the parasites & parasitic diseases of camel population in India were of the opinion that Sarcoptic mange is a serious, debilitating, dreaded and widely prevalent disease of camels in India. Besides other infectious diseases of bacterial and viral origin, camels are exposed to a wide range of internal & external parasitic infestations. Amongst other so many external parasites to which camels are exposed, the Sarcoptic mange is recognized to be one of the most Introduction 3 serious and damaging disease. This disease is caused by a mite known as Sarcoptes scabiei var cameli which belongs to genus Camelus of SARCOPTIDAE family in Veterinary Entomology. It is an extremely pruritic, contagious and debilitating skin disease which is very frequently and sudden in onset. It is also ranked as one of the most serious and important disease of the camels. Sarcoptic mange infestation is very common in the areas of thin skin, the head, neck, flanks, medial aspect of thighs or inguinal region, mammary glands and prepuce. The head is usually affected very rapidly as the animal uses its teeth for scratching the affected areas. Besides linking the occurrence of the disease with poor camel management, malnutrition and contact with infected objects, the stray & infected camels also often become a focus of infecting the healthy animals when mingling with them particularly at watering places for drinking purpose (Richard, 1987; Abdel-Rehman et al. 2001). Sarcoptes is a burrowing mite as it penetrates deeply through the skin surface of the infected camel. This burrowing of mites in the skin helps these parasites lead to intense pruritus and exudative dermatitis. In pruritus, mites penetrate deep into muscular areas, damaging the flesh and lowering the quality of meat. The early inflammatory reaction of the host body towards the mites becomes evident in the shape of small popular elevations, invasion and injuries leading to formation of hairless areas, scaly crust formation or scabs on the affected parts and the skin become dark and thickened. Skin of mangy camel show hemorrhages, and subcutaneous odema after the development of fissures in the underlying epidermis (Kumar et al. 1992; Amer et al. 2006). The fertilized female mites create winding burrows or tunnels in the upper layers of the epidermis of the skin of the host animal and feeding on the serous exudate, a liquid oozing from the damaged tissues. The female mites lay about 40-50 fertilized eggs in these tunnels which Introduction 4 hatch in 3-5 days into a six legged larvae. These larvae immediately crawl to the surface and burrow themselves in the superficial layers of the skin and create small molting pockets. In these molting pockets, the larvae molt to next stages of nymph and adult. The adult male then emerges and seeks a female either in the molting pocket or on the surface of skin. After fertilization the female produces new tunnels, either de novo or, by extension, of the molting pockets, lays eggs in these tunnels and a new life cycle starts. The entire life cycle of Sarcoptic mange is completed in 17-21 days. New hosts can be infected through direct transmission by contact between the animals, presumably from larvae, nymph or adult mites, which are commonly present on the skin surface of the infected animal. Indirect transmission of infestation can also take place through the objects or fomites having mange infection, which come into contact with the affected camel, such as harnesses, blankets, baggage tack, tents and tree trunks (Richards, 1987). The pruritus increases as the mites penetrate deeper in the skin (Al-Rawashdeh et al. 2000, Driot et al. 2011, Bekele et al. 2012). Based on the rate of infection camels can be seriously disturbed by the Sarcoptic infestation as they may stop grazing which can lead to a rapid fall in milk production, and deterioration of health condition. With the increase in the irritation due to scabies, the camel rubs, bites and scratches the affected areas in an attempt to reduce the itchiness. Due to rubbing, biting or scratching, the mites move to the periphery affecting the healthy tissues and resultantly affected area spreads. As the disease prolongs, the skin becomes excoriated, leading to hair loss and the development of scabs. These scabs in turn may be rubbed away and a red surface developed. The animal becomes restless due to severe Sarcoptic mange infestation and involvement of most of the body surface. If the diseased animal is not treated in time, the animal loses its health condition, become emaciated and within two, three weeks the acute stage of Introduction 5 disease may give way to more chronic state (Gorakh et al. 2000, Abubakar et al. 2002, Driot et al. 2011). Sarcoptic mites rarely survive long off the host under natural conditions. A continuous direct contact of animal keepers with their camels can also lead to transmission of diseased condition in human beings which is termed as pseudo scabies. Transmission of infection from camel to man usually takes place during milking, handling or riding. The main symptoms of pseudo scabies can therefore be seen in the inter digital spaces of the hands, on the wrists, forearms, the elbows, the axillary folds and inner side of the thighs. Once a herd is infected with Sarcoptic mange, continuous reinfection of the disease occurs (Schillinger 1987, Singh & Veer 2005, Premalatha et al. 2010). Sarcoptic mange is usually considered to be a seasonal disease and is often reported severe during the winter months as in cold weather the disease had an acute course. However, there is some evidence that in some countries hot weather predisposes to acute outbreaks of camel mange and in the cooler, winter season the rate of mange infestations are at the lowest. In the summer the activity of the mite seems to decline or disease becomes chronic. Dietary intake is an important factor in mange infestation. Nomadic camels on a low nutrition plan, probably carrying heavy worm burdens in hot desert conditions are likely, therefore, to be highly prone to Sarcoptes at this time (Dinka et al, 2010). During such periods of great activity, the mites are readily transmissible from one animal to other animals (Richards, 1987, Banaja & Ghandour, 1994, Tefera & Gebreah, 2001). Mange can easily be diagnosed clinically from the occurrence of pruritus, depilation, alopecia, thickened skin, folds around the joints and encrusted plaques being the main characteristics of this parasitosis. In order to control this zoonotic disease, it is essential to treat Introduction 6 both camel and man along with effective checks over other predisposing factors of the disease such as hygiene and nutritional requirements of the animals. The skin diseases like the scabies both in human beings and animals are being treated with a variety of allopathic drugs now a day, but the role of herbal plants in use since centuries in different shapes cannot be ignored at all, especially in the rural lifestyle. Further with the continuous use of different acaricidal drugs, the issue of resistance development has come across as a challenge for the researchers to find some alternatives for the purpose. Accordingly the research work on the use of traditional herbal medicines is gaining attention day by day. Although there are many reports and studies regarding the prevalence of Sarcoptic mange in camel from different parts of the world, only few preliminary reports are available for Pakistan and none of them provide detailed epidemiology of Sarcoptic mange and its effect on host health. Therefore, keeping in view the importance of the mange problem in camel population of the country, the present project was designed to determine the prevalence of Sarcoptic mange infestation, factors in its occurrence its zoonotic importance, effect on blood physiology and different treatment options in the camel population of Punjab, province in Pakistan. Availability: Items available for loan: UVAS Library [Call number: 2190,T] (1).

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