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1. Effect Of Different Doses Of Bovine Somatotropin Hormone In-Ravi Buffaloes On Growth, Milk Production and Physiological Parameters

by Hafiz Faisal Majeed | Prof. Dr. Makhdoom Abdul Jabbar | Dr. Muhammad | Prof. Dr. Talat Naseer Pasha.

Material type: book Book; Format: print Publisher: 2007Dissertation note: The present study was conducted to see the effect of different doses of bovine sornatotropin hormone (bST) on milk production, milk composition, growth! body weight, and physiological parameters (Hemoglobin, Total protein, 1-lernatocrit values, Cholesterol, Glucose, Urea and ESR level). For this purpose 21 Nili-Ravi lactating buffaloes were involved in this experiment. They were kept at Livestock Experimental Station Bahadur Nagar Okara with similar stage of lactation were selected. They were randomly divided in to three groups A, B and C with 7 animals in each group. The group A was injected with full- recommended dose of bST hormone (250 mg /animal) with trade name of Boostin-250 S/C at an interval of 14 days, while the animals of group B were given S/C injection on alternate days with divided doses of(36 mg/animal). The group C was kept as control. The maintenance requirement of the animals was met through by available green fodder at the rate of 40-50 kg per animal per day while production requirements were met through concentrate ration at the i-ate of half of the milk production. Milk production was recorded daily and milk sarnpes weve coXeeted on weakly basis or analysis. The blood samples were collected on fortnight basis to see the effect of bovine Sornatotropin hormone on physiological parameters. The research trial continued for 6 month till the completion of lactation. The average (rnean± SD) milk production of groups A, B and C were 7.38±1.30, 7.29±1.20 and 6.66±0.80 litters respectively. The increase in milk production was found to be 11% as compared to group C (control). The average (mean± sd) Fat, SNF and T.S percent in animals of group A were recorded 6.59±0.10, 9.13±0.28 and 15.73±0.34, respectively Where as in group B it was 6.58±0.15, 9.28±0.26 and 16.01±0.30 respectively. The averages (mcan±SD) Fat, SNF and TS percent in control group C were, 6.42±0.111, 8.83±0.47 and 15.45 ±0.245 respectively. There was improvement in Fat, SNF and TS percent of the treated group versus control group. The average (mean±SD) body weight found in subgroups A, B and group C was 528.78±50.64, 561.02±91.35 and 518.06±37.50 kgs respectively. There was slight increase was observed in treated group. The average values of Hemoglobin, 1-Iaernatocrit, ESR, Cholesterol, Total protein, lipid glucose, and Urea in experimental animals of group A were I 1.44g/dl, 32.84%, 94.15mm/h 173.87mg/lOOrni, 10.99g/dl, 567.57 g/dl, 86.25 mg/dl, and 4.089 rng/dl and respectively. In animals of group B there were 11.96 g/dl, 32.04%, 74.70 mm/h 181.10 mg/lOOm!, 11.10 g/dl, 548.03 g/dl, 84.655 mg/dl and 3.7474 rng/dl respectively. Whereas in-group C (control) the average values of Hemoglobin, Haernatocrit, ESR Cholesterol, Total protein, lipid glucose, Urea were 12.20 g/dl, 33.11%, 83.11 mm/h 190.78, mg/lOOm!, 10.60 g/dl, 557.32 rng/dl, 84.92 rng/dl and 3.8848 g/dl, respectively. It is concluded that the bovine Somatotropin hormone had no desirable effect on physiological parameters. It is concluded that treatment of bovine sornatotropin hormone had no effect on blood composition, milk composition and physiological parameters in Nili-Ravi buffaloes. It is also concluded that dose of bSt of 250mg injected at 14 days interval is beneficial for overall performance of Nili-Ravi buffaloe. To overall bST had positive effect on economic parameters in Nili-Ravi buffaloes. Availability: Items available for loan: UVAS Library [Call number: 0950,T] (1).

2. Effect Of Sodium Bentonite On Growth Performance And Feed Efficiency

by Ghayur Ahmad Khan | Prof. Dr. Makhdoom Abdul Jabbar | Prof. Dr | Prof. Dr. Talat Naseer Pasha.

Material type: book Book; Format: print ; Nature of contents: biography; Literary form: Publisher: 2007Dissertation note: A trial was conducted to determine the effect of supplementing sodium bentonite, to find the effectiveness of sodium bentonite on the growth performance of Sahiwal male calves. Twelve animals of almost same age and weight were selected from the available stock at Animal Nutrition Centre, Rakh Dera Chahl, Lahore. These animals were divided into three groups and fed a standard ration "A" according to their nutrient requirements. Rations of group B and C were supplemented with sodium bentonite at the rate of 2.5 and 5.0 per cent while group A served as control. The trial was run for a period of 90 days with normal managemental practices. During the experimental period body weight gain and feed consumption of the individual animals was recorded. At the end of the experiment the data were analyzed statistically. The result of the experiment suggested that addition of sodium bentonite had statistically non-significant effect on the body weight gain, feed consumption, and feed conversation ratio and rate of passage of digesta through the gastro-intestinal tract. Based on the results of this trial it could be inferred that supplementation of sodium bentonite may have some beneficial effect on the performance of calves. However, use of this clay over extended period of time needs to be investigated for any adverse effect in future Availability: Items available for loan: UVAS Library [Call number: 0968,T] (1).

3. Impact Of Various Feeding Regimes On Growth Performance Of Male Buffalo Calves

by Rashid Iqbal | Prof. Dr. Makhdoom Abdul Jabbar | Prof. Dr | Prof. Dr. Anjum Khalique.

Material type: book Book; Format: print Publisher: 2011Dissertation note: There has been a gradual decline in per capita availability of proteinous food particularly of animal origin for human consumption in our country. The feasible solution of the problem is to increase the production of meat. The potential of buffalo as a food animal to meet present and future demands of rapidly growing population deserves special attention because of its capacity to economically convert course roughages and other cereal by products into meat. Pakistan is second largest buffalo holding country in the world having 29 million buffalo. Traditional system of buffalo meat production is inefficient; animals destined for slaughter are mostly culled and old stock. Male calves are not reared specifically for producing meat but they are kept only for milk let down in villages and are slaughtered even at 6-7 days of age in pen-urban areas. So far very little work has been done to evaluate the tremendous meat production potential of buffalo calves with a particular plan of nutrition. Present study consisted of a feeding trial of about three months in duration. Fifteen Nih Ravi calves of approximately the same age and weight were selected for the subject study. The animals were randomly divided into three equal groups which were reared on three different feeding regimes. Group I was fed TMR ad-libitum and 2 kg of green fodder. Group II was fed available green fodder ad-libitum and concentrate ration @ 1% of body weight. Group III was fed Lucerne hay ad-libitum and concentrate ration @ 1% of body weight. . Weighed quantity of feed was offer to every animal on individual feeding basis in a group and refusal was also recorded individually. The results of study revealed that animals fed on TMBR performed better as compared to other feeding regimes. Among three feeding groups, highest (124.60 ± 7.82 kg) weight gain was observed in TMBR group while, lowest (108.20 + 5.80 kg) weight gain was found in Hay group. As far as feed intake is concerned, highest (777.42 + 8.87 kg) feed intake was observed in fodder group while, lowest (755.10 ± 16.61 kg) feed intake was found in TMBR group. Out of three feeding groups, calves fed on TMBR showed best feed conversion ratio (6.07 ± 0.47) while, the feed conversion ratio in calves fed on hay and fodder was 7.04 ± 0.39 and 7.01 ± 0.32 respectively. Economic analysis per kg weight gain revealed that TMBR group had the lowest cost of production in rupees (101.43 ± 7.92) per kg weight gain, where as highest cost of production (130.40± 7.40) was found in hay group. Analysis of variance of serum chemistry means revealed that no significant differences were found in total protein, urea, glucose, and creatinine values for all three feeding regimes. On the basis of results of this study it is concluded that TMBR can be utilized as an alternate for our conventional feeding practices as it can meet all nutritional requirements of the animal. It is easy to handle, transport and store besides having a longer shelf life. It is suggested that further trials with TMBR be carried out on different age and sex groups of buffalo and cattle in order to evaluate its effects on the performance of the animals. Availability: Items available for loan: UVAS Library [Call number: 1227,T] (1).

4. Effect Of Different Energy Sources On The Fattening Potential Of Buffalo Calves

by Mujahid Jamal Jami | Prof. Dr. Makhdoom Abdul Jabbar | Dr.Abdul | Prof.Dr.Talat Naseer Pasha.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2011Dissertation note: Buffalo is an important animal which contributes approximately 50% meat in Pakistan. Traditional system of meat production is inefficient. About 50 % of the male calves die during the first week of their life. Some calves are raised to 60-80 kg on extremely poor and unbalanced diets and only a few are raised to about 200 kg of live weight with very low average daily gains. Most of the meat produced in the country comes from end of career, or emergency slaughtered animals. To improve meat production in country calves are required to be fattened through feed lot system. Energy-rich sources available in Pakistan include cereal grains (wheat, maize, barley, oats, sorghum, and rice), wheat bran, rice polishing and molasses etc. At the same time fats and oils are also rich source of energy and infrequently used in animal feeding. Molasses, a by product of sugar industry, is an excellent and cheaper source of energy. An objective of the study was to compare efficiency of energy sources for meat production. For this purpose, a fattening trial was conducted on 15 Nili-Ravi buffalo calves of approximately the same age and weight to study the comparative efficacy of three different energy rations. Animals were divided into 3 groups of 5 calves each. Animals were offered three different rations. The experiment was carried out for 90 days. Group A was fed TMR having molasses as energy source, group B was fed TMR having com grain as energy source and group C was fed TMR with rumen protected fat as energy source. Daily feed consumption, body weight gain, feed conversion ratio, economics and blood parameters (blood glucose, total protein, urea and creatinin) were studied. The Data obtained was analyzed under CRD through ANOV A and the difference among treatment means were tested with LSD. The average weekly growth rate of group A, B and C was 6.23±0.187, 6.l85±O.l87 and 6.08±0.187 respectively. The average daily growth rate of group A, Band C was 0.89, 0.88 and 0.87 kg respectively. Total Mixed Ration taken by group A, B and C was 2064.5 kg, 2546.01 and 1993.7 kg respectively. The average daily feed intake was 4.59 ±O.l06, 5.66±0.106 and 4.43±0.106 kg per day of group A, B and C respectively. Group A, C had non significant difference (P>0.05) from each other while group B was significantly different from group A and C. The feed conversion ratio of group A, B and C was 5.16 ±0.25, 6.40±0.25 and 5.10±0.25 respectively. The results showed that calves fed ration C has highest FCR than ration A and B. The results of the study revealed non significant difference in the concentration of serum urea, creatinine, glucose and serum total protein among the experimental animals. The cost of one kg live weight for group A was Rs 78.73 while Rs 102.78 and Rs 129.1 for group Band C. The results showed th~t ration A was more economical than ration Band C. The findings of this study showed the benefit of utilization of different energy sources in the fattening ration of calves. Due to higher prices and demand of cereal grains, other cheaper sources such as molasses can be used for fattening as energy source. If there is unavailability of molasses and cereal grains then bypass fat (Berga fat) can be used for fattening purpose. The study revealed that each energy sources is equally effective. Availability: Items available for loan: UVAS Library [Call number: 1268,T] (1).

5. Effect Of Feeding Citrus Pulp Silage On The Growth Performance Of Sahiwal Calves

by Muhammad Iqbal | Prof.Dr.Talat Naseer Pasha | Prof. Dr. Makhdoom Abdul Jabbar.

Material type: book Book; Format: print ; Nature of contents: biography; Literary form: Publisher: 2011Dissertation note: Livestock is contributing about 53 per cent of agricultural value added and 11.4 per cent to the national GDP with a total animal heads of 157.3 million but feed availability of crude protein (CP) and total digestible nutrients (TDN) is deficient by 38.10 and 24.02%, respectively. However, a decline in domestic feed resources and the need for Pakistan's livestock production to become internationally competitive are strong reasons for promoting the use of alternative locally available and cheap sources of feed ingredients, such as citrus pulp from fruit processing industry which do not attract competition in consumption between humans and livestock. The primary objective for the use of these by-products is to reduce the feed cost and consequently cost of producing a unit of the product like milk or meat as well as to fulfill the nutrients demands of animals. Citrus pulp is a by-product widely available in Pakistan and is mainly discarded as waste. Its cost is relatively low compared to its nutritive value but is seldom used as ruminant feed. The large amounts which are available during the harvesting season can be ensiled in combination with dry roughage, to get the required moisture level of it. So in the present study citrus pulp with wheat straw was ensiled and the silage was fed to Sahiwal calves to observe its intake, weight gain as well as economics. Results of the present study revealed that the optimum level of 20% citrus pulp in feed gave good results while intake and weight gain decreased for 30% citrus pulp in feed. Average total weight gain were 27.5, 37.5, 28.75 and 23.75kg for ration A, B, C and D, respectively (containing 10, 20 and 30 citrus pulp, respectively). Average daily gain for group A, B, C and D were 0.44, 0.60, 0.46 and 0.38kg, respectively and the difference was statistically significant (P < 0.05). When average daily feed intake for each calf was calculated on dry matter basis it showed 4.40, 4.50, 3.39 and 3.13kg respectively for group A, B, C and D which was not significantly different between diets. By ascertaining the cost of the feed for ration groups it showed significance difference having average cost of production per animal per kg body weight 37.07, 23.80, 21.68 and 25.58 Rs for group A, B, C and D, respectively. Availability: Items available for loan: UVAS Library [Call number: 1284,T] (1).

6. Comparative Growth Rate And Body Composittion Of Major Carps (Labio Rohita , Cata Catla And Cirhinus Mrigala )

by Noor Khan | Prof . Dr . Grant William Vandenberg | Prof . Dr . Makhdoom Abdul Jabbar.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2011Dissertation note: Presently fish culture in Pakistan is primarily dependent on natural food produced in pond by the application of organic and inorganic fertilizers. It is supplemented with cheaper agriculture by-products to meet the nutrient deficiencies. Artificial feed which is a blend of various plant and animal by-products is rarely used. Development .of appropriate artificial feed now has become mandatory to transform conventional fish culture practices to advanced fish production systems to improve per unit fish production. The present study was therefore signed to formulate a quality supplementary feed from cheap and easily available feed ingredients that contains at least minimum required nutrients for different age groups (fingerlings and grow-out). The feeds developed during these studies were evaluated in terms of growth, diet utilizalion efficiency and its effect on the body composition and flesh quality of the three Indian majr carps (Catla cat/a, Labeo rohita and Cirrhinus mrigala).The study comprised of three trials. Trial I was conducted on fingerlings of individual species under monoculture system using 42% protein diet. Trials II and III were conducted on Grow-out fish using 35% protein diet under monoculture and polyculture systems. The study was conducted in earthen ponds having an area of 0.03 ha with three replicates and a control. After preliminary preparation of ponds, in trial I, fingerlings were stocked at 80 fish per pond. while in trial II at 70 fish of each species and in trial III ratio of 30%, 50% and 20% of Catla catla, Labeo rohita and Cirrhinus mrigala per pond were maintained. All the ponds received same amount of organic and inorganic fertilizers (cow dung, poultry manure, SSP and urea) thoughout the experimental period. Supplementary feed in trial I was applied at 4% of fish wet body while in trial II and III feed was applied at 3% of fish wet body weight daily. In trial I 42% protein diet was used containing fish meal. soybean meal. maize gluten (60%). rice polish, wheat bran. maize grains. molasses. vitamins and minerals while in trial II and III 3YYo protein diet containing fish meal, soybean meal. canola meal. rice polish. wheat bran, molasses, vitamins and mineral was used. Growth parameters in terms of length and weight gam were regularly monitored fortnightly. Organolept sensory evaluation was done at the termination of each trial. Proximate fish body composition was determined at the start and at the end of the experimental trials. Fatty acid profile of three experiments was performed at the post-trial basis. In addition, specific growth rate (SGR), feed conversion ratio (FeR). protein efficiency ratio (PER). protein utilization (PU). gross nitrogen retention efficiency (G RE %) and gross energy retention efficiency (GERE %) were also determined. Proximate analysis of feed ingredients and formulated diets was also done. Key physico-chemical parameters viz. temperature, dissolved oxygen (DO), free CO2, pH, electrical conductivity, total dissolved solids, light penetration. salinity and nitrates, were regularly monitored during the study period. In trial I the highest net weight gain was observed in treatment group (D 1) (Catla calla 9425.83 g and 171.5 mm) followed by Labeo rohita (374.34 g and 178.7 mm) and Cirrhinus mrigala (288.18 g and 161.9mm). The lowest growth was observed in Cirrhinus mrigala (176.9 g and 116.4 mm) in control (DO). A significant difference was observed regarding net weight gain among three fish species and between different treatments (DO and 0 I). The net weight gain was significantly higher in trial I treated (01) ponds than control (~O). Percent weight gain and specific growth rate (SGR %) were also determined. Labeo rohita exhibited higher values (1762.51 % and 3.03%) followed by Catla calla (1341.58% and 2.95%), while Cirrhinus mrigala showed lowest (976.17% and 2.6%) with experimental diet (DI) Again Cirrhinus mrigala exhibited lowest percent weight gain and SGR (300.85% and '1.54%)in control (DO) ponds. In trial II grow-out under monoculture the net weight gain of fish differed significantly among three fish species and between treatments (DO and D2). Calla catla showed highest net weight gain (37\.88 g and 72.2 mm) followed by Labeo rohita (310.18 g and 72.3 mm) and Cirrhinus mrigala (270.75 g and 57 mm) in experimental unit (02) while a lowest net weight gain of Cirrhinus mrigala (162.15 g and 36.5 mrn ) was observed in control (DO). Percent weight gain and specific growth rate of three fish species Catla catla, Cirrhinus mrigala and Labeo rohita under different treatments were found non-significant. Although Catla catla showed highest percent weight gain and SGR values (109.78% and 0.81 %) followed by Labeo rohita (90.93% and 0.69%) and Cirrhinus mrigala (84.3% and 0.65%), respectively with experimental diet (D2). Lowest values of percent weight gain and SGR (48.54% and 0.43%) were observed for Cirrhinus mrigala in control ponds (DO). In trial III grow-out under poly culture the average final weight of fish was significantly different in control (~O) and experimental diets (02) while species showed non-significant difference regarding final weight and net weight gain. The highest final and net weight gain of Lobeo rohita (679.46 g and 370.5 g) followed by Cirrhinus mrigala (674.52 g and 303.86 g ) and Catla catla (607.2 and 307.06 g), respectively in experimental unit (D2) while Catla catla exhibited lowest final weight and net gain in weight (493 g and 182.3 g) in control (DO). Regarding percent weight gain and specific growth rate of three fish species under polyculture system no significant difference was observed hence, Labeo rohita showed highest percent weight gain and SGR (126.87% and 0.9%) followed by Catla catla (l 02.31 % and 0.76%) and Cirrhinus mrigala (85.15% and 0.63%), respectively with experimental diet, while Cirrhinus mrigala once again showed lowest values (40.12% and 0.37%), respectively in control diet (DO). Feed conversion ratio (FCR), protein efficiency ratio (PER), protein utilization (PU), gross nitrogen retention efficiency (GNRE %) and gross energy retention efficiency (GERE %), in all the three experiments under monoculture as well as in polyculture system, for fingerlings and grow-out fish of three species were found non-significantly different. However, in trial I fingerlings better FCR values (1.63, 1.56 and 1.43) were obtained for Catla catla, Cirrhinus Mrigala and Labeo rohita. Regarding gross nitrogen retention efficiency Catla catla showed highest GNRE % value (10.4) followed by Labeo rohita (9.3) and were found significantly different from Cirrhinus mrigala (6.5) in experimental unit. In trial II grow-out monoculture, FCR values 3.7. 4.57 and 4.56 for Calla calla. Cirrhinus mrigala and Labeo rohita were pbtained while GNRE % varied 9.5,5.8 and 8.0. respectively. In trial III grow-out poIyculture the FCR values of three species varied from 3.99, 4.72 and 3.61, respectively while GNRE % varied from 10.3, 8.2 and 12.5%, respectively among Calla catla, Cirrhinus mrigala and Labeo Rohita. The Labeo rohita for GNRE% differed significantly from other two species. No significant difference among species and between diets (DO, D 1 and D2) was observed in proximate composition in all the three experiments. However, in case of fingerlings Labeo rohita under experimental diet (D 1) showed higher protein contents (16.44<Yo) while Catla catla showed the lowest protein content (12.9%). Crude fat contents were found highest (7.28 %) in Labeo rohita with control diet (DO) followed by Cirrhinus mrigala (6.96 %) and Labeo rohita (6.S2 %) in experimental diet (01) while lowest values were observed for Calla catla (4.17%) in control (DO). The Ash contents showed minor variations among species and treatments ranged from (4.81 % and 3.S6%) for Catla catla, (4.34% and 4.7S%) for Cirrhinus mrigala and (3.98% and 4.49%) for Labeo rohita in control and treated ponds, respectively. Highest gross energy was found (6.S3MJg'l) for Labeo rohita and lowest (S.OMJg'l) for Catla catla with experimental diet (D 1). In trial II grow-out monoculture the highest crude protein contents (1S .16%) were observed in Labeo rohita followed by Cirrhinus mrigala (14.S3%) with control diet (~O) while lowest for Labeo rohita (12.13%) in (02). Higher contents of crude fat (7.31 %) were observed in Cirrhinus mrigala followed by Catla catla (S.38%) in experimental group and lowest amount 3.18% and 3.19% was observed for Cirrhinus mrigala and Catla catla in control group (~O) . . Higher amount 4.11 % was found in Catla catla under control (~O) while lowest amount 3.1 % was observed in Labeo rohita under experimental diet (D2). Highest gross energy percentage 996.13%) was observed for Cirrhinus mrigala under experimental diet (D2) while lowest 4.91 % was observed for Catla catla in control group (DO). In case of experiment III grow-out polyculture the proximate body composition highest crude protein contents (IS.76% and 10.53%) were observed for Cirrhinus mrigala followed by catla catla 911.87% and 13.3S%) and Labeo rohita (12.72% and 6.S6%) in treated (D2) and control (DO) group. respectively. Higher crude fat contents (6.S7%) were observed in Cirrhinus mrigala under (D2) while lowest (3.13%) in Labeo rohita and (2.9S%) in Catla catla. Ash percentage was found higher in Catla catla and lowest (2.14%) in Labeo rohita and Cirrhinus mrigala (2.87%) under (DO). Gross energy contents were found highest (6.84MJg,l) in catlacalla under (DO) and (6.56MJg,l) Cirrhinus mrigala under (D2) while lowest amount (3.24MJg.l) were observed in Labeo rohita under (DO). Mineral composition of three fish species under three dfferent experiments showed non- sign ificant differences. Minor variation regarding mineral composition was observed in pre- treatment and post-treatment level. However. Ca and P contents showed relatively higher percentage than Mg and K contents in all the three experiments. A significant difference was observed in Mg contents in experiment III where Catla catla showed significantly higher (0.045%) percentage than Cirrhinus mrigala and Labeo rohita each containing 0.02%. A significant difference was observed in fatty acid profile among three fish species and between diets (~O, Oland D2). Among fatty acids, palmitic acid (C 16:0) was found a dominating fatty acids in all the three experiments. In trial I highest concentration (40.59 g 100 g-1 was found in Cirrhinus mrigala under (DO) and 37.19 in (D1) while lowest (30.75 and 30.78 g 100 g.l) in Labeo rohita and Catla catla under (D 1). The concentration of total saturated fatty acids were observed higher and ranged from (40.20 to 53.29 g 100 g-I) followed by total monounsaturated fatty acids (29.30 to 37.81 g 100 g-I), w-6 PUFA (7.65 to 14.94 g 100 g') and @-3 PUFA (7.76 to 11.07 g 100 g-I). respectively. In case of trial II significant differences were also found among three fish species and diets (D0 and 02) for different fatty acids composition. Palmitic acid (C 16:0) also showed highest concentration ranged from 28.36 to 29.73 g 100 g-I). Total saturated fatty acids were found higher that varied from (35.90 to 39.41 g 100 g-I) followed by total monounsaturated fatty acids (36.52 to 40.84 g 100 g-I), and l:PUFA (19.02 to 24.40 g 100 g-I), respectively. In trial III once again same pattern of dominance of palmitic acid along with total saturated fatty acids (36.43 to 42.24 g 100 g-I) followed by total monounsaturated fatty acids (36.899 to 43.72 g 100 g-I) and 2:PUFA (14.97 to 23.03 g 100 g-I) were observed. In case of organoleptic evaluation all the species under di Iferent culture system and treatments illustrated non-significant differences. Hence. significant differences were observed among different cooking processes (steamed and fried fish). The physico-chemical parameters of pond water remained within the acceptable limit for Fish gowth. Although comparatively lower values of temperature were found for experiment II and III for grow-out trial that was conducted in fall. The correlation co-efficient studies revealed a positive significant correlation of temperature, TDS, light penetration and salinity with growth of fish species while pH showed positive non-significant correlation with growth of fish. It was concluded from the present study that both the experimental diets D I and 02 for different age groups (fingerlings and grow-out) showed significantly higher growth of all the three species in monoculture system. The diet D2 did not showed any significant higher growth in polyculture system but overall growth performance remained high in polyculture than monoculture treated ponds of grow-out fish. Comparison of species indicated that artificial diets (DI and D2) remained much suitable for Catla catla and Labeo rohita than Cirrhinus mrigala under both the culture systems. Non-significant difference was observed in the body composition and flesh quality irrespective of their economic viability. Information derived from the present research experiments will be useful in future research and formulating supplementary feed for Indian major craps for different age groups. It can also be helpful in understanding the mineral and fatty aeid profiles of the Indian major carps cultured under semi-intensive pond culure system whieh is first study of its kind on these species in Pakistan. Availability: Items available for loan: UVAS Library [Call number: 1290,T] (1).

7. Nutritional Evaluation Of Locally Availabie Feed Stuffs In Different Agro- Ecological Zones Of Punjab

by Muhammad Arif | Prof. Dr. Makhdoom Abdul Jabbar | Prof. Dr. Akram | Prof. Dr. Talat Naseer Pasha.

Material type: book Book; Format: print Publisher: 2011Dissertation note: Pakistan's economy is mainly based on agriculture and livestock production. The production per unit animal in terms of meat and milk production is low as compared to developed countries. The major reason behind this truth lies in efficient use of feed resources. In Pakistan, availability of data on indigenous feed resources in different agro-ecological zones is scanty and limited. Present study was planned to analyze the nutritional profile of locally available feed resources in different agro-ecological zones of Punjab. This study was conducted into two phases; first phase consisted of collection of samples from ten districts of Punjab. From each district, sample of fodder, dry roughages and concentrate ingredients were collected randomly from different places. The estimated total number of sample was 1020; fodder (440), dry roughages (250) and concentrate (330) samples were collected in both summer and winter seasons. The second phase of the study consisted of chemical analysis of nutritional profile of collected samples. Samples were analyzed for crude protein, ash, detergent fibres i.e., NDF, ADF, cellulose, hemi-cellulose, lignin and cell soluble contents. Data thus collected were subjected to statistical analysis using analysis of variance technique under CR Design. In wheat straw the average CP percentage was 2.20 ± 0.25% while the value for ash content was 9.79 ± 1.97%. NDF and ADF averaged as 80.33 ± 4.43% and 52.41 ± 2.56% respectively. Higher value for protein was reported from Kasur and Sahiwal district while lowest from Sheikhupura district. In the rice straw average CP percentage was 1.71 ± 0.24% while the value for ash content was 13.56 ± 1.91%. NDF and ADF averaged as 78.36 ± 5.40% and 54.11 ± 2.25%. Higher value for protein was reported from Sahiwal district while lowest from Lahore and Pakpatan district. In the maize stovers average CP percentage was 2.76 ± 0.39% while the value for ash content was 7.81 ± 2.16%. NDF and ADF averaged as 75.03 ± 4.78% and 48.15 ± 3.67% respectively. Higher value for protein was reported from Kasur district while lowest from Pakpatan district. In the sorghum stovers average CP percentage was 2.72 ± 0.15% while the value for ash content was 5.81 ± 1.16%. NDF and ADF averaged as 77.74 ± 4.60% and 49.95 ± 2.31% respectively. Higher value for protein was reported from Sahiwal district while lowest from Hafizabad district. In the millet stovers average CP percentage was 3.03 ± 0.37% while the value for ash content was 6.45 ± 0.72%. NDF and ADF averaged as 75.80 ± 4.45% and 43.81 ± 0.94% respectively. Higher value for protein was reported from Hafizabad district while lowest from Kasur district. In the rice husk average CP percentage was 2.99 ± 0.22% while the value for ash content was 16.37 ± 0.58%. NDF and ADF averaged as 78.90 ± 2.90% and 69.01 ± 1.17% respectively. Higher value for protein was reported from Pakpatan district while lowest from Kasur district. In the oat fodder average CP percentage was 11.89 ± 0.74% while the value for ash content was 10.68 ± 0.57%. NDF, ADF, lignin and CSC averaged as 63.09 ± 1.88%, 32.68 ± 2.33%, 4.26 ± 0.81% and 36.91 ± 1.88% respectively. Higher value for protein was reported from Okara district while lowest from Sheikhupura district. In the wheat fodder average CP percentage was 11.74 ± 0.51% while the value for ash content was 6.84 ± 0.78%. NDF, ADF, lignin and CSC averaged as 66.65 ± 2.70%, 36.31 ± 2.08%, 9.40 ± 1.72% and 33.35 ± 2.70% respectively. Higher value for protein was reported from Lahore district while lowest from Khushab district. In the mustarad fodder average CP percentage was 13.42 ± 0.39% while the value for ash content was 11.57 ± 0.66%. NDF, ADF, lignin and CSC averaged as 56.19 ± 1.94%, 38.49 ± 1.98%, 12.24 ± 0.98% and 43.81 ± 1.94% respectively. Higher value for protein was reported from Muzafargarh district while lowest from Pakpatan district. In the berseem average CP percentage was 17.32 ± 0.82% while the value for ash content was 12.94 ± 0.76%. NDF, ADF, lignin and CSC averaged as 57.37 ± 2.63%, 31.63 ± 1.90%, 10.67 ± 1.04% and 42.63 ± 2.63% respectively. Higher value for protein was reported from Pakpatan district while lowest from Hafizabad district. In the lucern average CP percentage was 21.34 ± 0.84% while the value for ash content was 11.65 ± 1.69%. NDF, ADF, lignin and CSC averaged as 45.60 ± 2.04%, 37.73 ± 0.63%, 11.87 ±1.05% and 54.40 ± 2.04% respectively. Higher value for protein was reported from Hafizabad district while lowest from Sahiwal district . In the sugarcane fodder average CP percentage was 5.60 ± 0.31% while the value for ash content was 11.78 ± 0.92%. NDF, ADF, lignin and CSC averaged as 72.30 ± 3.88%, 38.20 ± 1.76%, 12.94 ± 1.13% and 27.20 ± 3.00% respectively. Higher value for protein was reported from Sheikhupura district while lowest from Lahore district. In the grasses average CP percentage was 9.91 ± 0.48% while the value for ash content was 11.74 ± 0.94%. NDF, ADF, lignin and CSC averaged as 72.71 ± 5.20%, 36.25 ± 1.96%, 6.29 ± 0.99% and 27.29 ± 5.20% respectively. Higher value for protein was reported from Lahore district while lowest from Sheikhupura district. In the maize fodder average CP percentage was 8.10 ± 0.75% while the value for ash content was 11.39 ± 1.58%. NDF, ADF, lignin and CSC averaged as 70.88 ± 1.94%, 32.88 ± 1.42%, 7.15 ± 1.87% and 29.12 ± 1.94% respectively. Higher value for protein was reported from Kasur district while lowest from Okara district. In the sorghum fodder average CP percentage was 6.62 ± 0.44% while the value for ash content was 8.96 ± 1.41%. NDF, ADF, lignin and CSC averaged as 67.75 ± 2.79%, 42.11 ± 2.64%, 7.52 ± 0.96% and 32.25 ± 2.79% respectively. Higher value for protein was reported from Okara and Khushab district while lowest from Kasur district. In the millet fodder average CP percentage was 7.76 ± 0.34% while the value for ash content was 14.80 ± 0.88%. NDF, ADF, lignin and CSC averaged as 69.90 ± 1.39%, 40.59 ± 4.54%, 9.74 ± 1.24% and 30.10 ± 1.39% respectively. Higher value for protein was reported from Sahiwal district while lowest from Khushab district. In the corn grain average CP percentage was 9.47 ± 0.22% while the value for CSC content was 75.54 ± 1.39%. While NDF and ADF averaged as 24.46 ± 1.39% and 5.88 ± 8.47%. Higher value for protein was reported from Lahore district while lowest from Okara district. In the wheat grain average CP percentage was 12.66 ± 0.89% while the value for CSC content was 59.75 ± 3.64%. While NDF and ADF averaged as 40.25 ± 3.64% and 4.86 ± 0.80%. Higher value for protein was reported from Lahore district while lowest from Gujranwala district. In the Sorghum grain average CP percentage was 9.96 ± 0.42% while the value for CSC content was 49.11 ± 3.10%. While NDF and ADF averaged as 50.89 ± 3.10% and 22.06 ± 0.72%. Higher value for protein was reported from Hafizabad district while lowest from Muzafargarh district. In the wheat bran average CP percentage was 12.53 ± 1.07% while the value for CSC content was 36.63 ± 2.24%. While NDF and ADF averaged as 63.37 ± 2.24% and 17.25 ± 2.63%. Higher value for protein was reported from Lahore district while lowest from Gujranwala district. In the rice polish average CP percentage was 16.12 ± 0.69% while the value for CSC content was 47.58 ± 2.34%. While NDF and ADF averaged as 52.42 ± 2.34% and 18.28 ± 0.80%. Higher value for protein was reported from Hafizabad district while lowest from Kasur district. In the cotton seed cake average CP percentage was 24.16 ± 0.36% while the value for CSC content was 40.91 ± 2.27%. While NDF and ADF averaged as 59.09 ± 2.27% and 27.65 ± 2.13%. Higher value for protein was reported from Khushab district while lowest from Sheikhupura district. In the rape seed cake average CP percentage was 35.77 ± 0.42% while the value for CSC content was 57.42 ± 2.41%. While NDF and ADF averaged as 42.49 ± 2.59% and 24.61 ± 2.08%. Higher value for protein was reported from Sheikhupura district while lowest from Pakpatan district. On the basis of the results of this study it is concluded that this study has generated compositional data of dry roughages, fodders and concentrates. This has lead to the preparation of dry roughages, fodders and concentrates composition tables for better feeding of livestock. The students, farmers and research workers may benefit from these tables. It is suggested that further trials on palatability, feed intake and digestibility should be conducted to visualize practically the extent of digested nutrients in these dry roughages, fodders and concentrates. Availability: Items available for loan: UVAS Library [Call number: 1320,T] (1).

8. Influence Of Early Weaning On Growth Performance, Plasma Metabolites And Rumen Fermentation Indices In Neonatal

by Muhammad Afzal Rashid | Prof. Dr. Talat Naseer Pasha | Prof. Dr. Makhdoom Abdul Jabbar.

Material type: book Book; Format: print ; Literary form: drama Publisher: 2013Dissertation note: Rearing of young calves is a labor intensive and costly segment of livestock production. From birth to weaning, young calf undergoes a transition from monogastric to adult ruminant. The concept of weaning from milk at an early age is based on early development of functional rumen enabling calves to utilize low quality roughages. In current study, a series of experiments were conducted to refine the more effective weaning regime for buffalo calves and use of by-products of the ethanol production industry in early weaned cattle calves. Conventionally, buffalo calves are kept with the dam, allowed to suckle a little amount of milk along with seasonal green forages, and weaned around the age of one year. To date, limited published work was available on growth performance and economics of buffalo calves weaned from milk at an early age. Therefore, the experiment was conducted to reduce the weaning age and evaluate the growth performance of male Nili-Ravi buffalo calves. Twenty-four male buffalo calves were assigned to one of the three treatments: continuous milk feeding (CMF), limited milk feeding (LMF), and early weaning (EW). After colostrum feeding, calves were individually fed whole milk at 10% of their BW, adjusted weekly until 6 wk of age. Thereafter, milk allowance was gradually tapered to zero in CMF, LMF and EW treatments at 12, 10 and 8 wk of age, respectively. Calf starter feed was provided ad libitum from wk 2 through wk 12 and individual intakes were recorded daily. Blood sampling was carried out form wk 6 through 12, on a weekly basis. The BW and structural measurements (HG, WH, and HW) were carried out at the start of experiment and later on a weekly basis. In young buffalo calves, the regimen of weaning at 8 weeks of age was more effective. The early weaned calves showed similar growth rate to those in the CMF and LMF by consuming more calf starter and saving a substantial amount of high priced milk. On the basis of the results of this experiment, buffalo calves successfully adapted to early weaning that might help to mitigate issues like poor growth and low returns associated with traditional calf rearing practices. Furthermore, this study effectively reduced the weaning age from 1 year to 8 weeks of age. Hence, reducing weaning age did not affect the growth performance of Nili-Ravi buffalo calves by 12 weeks of age. Early development of the rumen is the main objective of a successful early weaning program which depends upon the amount of starter intake, VFA production, and ruminal papillae development. Studies have shown that grains in starter feed can be replaced by DDGS up to 28% of DM without compromising the growth performance and rumen development. Second experiment was planned to evaluate the effects of replacing grains and soybean with DDGS and ammonia treated DDGS at 25% of DM. Study was conducted in collaboration with dairy science department SDSU (USA). Twenty one neonatal male Holstein calves were assigned to one of the three of dietary treatments: C = 0% DDGS, DDGS = 25% DDGS, CAFEX-DDGS = 25% CAFEX treated DDGS. In a 10 week experiment, calves were fed 680 g MR through 4 week, reduced to half during wk 5, and weaned at the end of wk 5. Starter intakes were conducted daily; whereas, body weights, structural measurements were conducted at the start of experiment and then on a weekly basis. Jugular blood samples were taken on a weekly basis using EDTA and NaFl coated evacuated tubes. Rumen samples were collected from a subset of 15 calves (n=5 calves/ treatment) at wk 5, 7 and 10. At the end of experiment, four calves from each treatment were also slaughtered to determine rumen morphometric measurements (PL, PW, RWT and PC). Experiment illustrated that weight gain, structural measurements, total starter intake, DMI and feed efficiency were not affected by the inclusion of DDGS and CAFEX treated DDGS at 25% of DM in starter feeds. CAFEX treatment of DDGS improved the CP contents of DDGS from 29.5% to 40%; however, inclusion of CAFEX-DDGS in starter reduced feed intake during the pre-weaning period. Whereas, overall starter intake was higher in calves fed DDGS based starter feed indicating the effect of ammonia treatment on palatability. Lower pre-weaning starter intake, slow rumen fermentation of CAFEX-DDGS resulted in lesser BHBA concentration leading to lesser development of rumen papillae growth (PL and PW). However, there was a tendency for higher weight gain in calves fed DDGS based starter due to increase in starter intake. In the light of these results it is concluded CAFEX-DDGS can be included in starter feeds at 25% of DM without affecting the growth performance. However, further research is required to evaluate the digestibility of DDGS after CAFEX treatement. Similar, growth performance indicates that CAFEX-DDGS can replace the corn and soybean meal in starter feeds. In third experiment, microbial diversity in developing rumen and intestine of young calves fed DDGS and CAFEX treated DDGS at 25% of starter was investigated. Experiement was carried out at SDSU dairy research station (USA). Fifteen calves with n=5 per treatment, fed according to protocols described in Experiment II. Calves were sampled for rumen contents at wk 5, 7 and 10 of age; whereas, intestinal contents were collected at the time of slaughter. The DNA was extracted subjected to PCR-DGGE and dendogram was constructed using cluster analysis software. Results revealed that microbial population was highly different from each other at wk 10 indicating the effect of age and dietary treatment on rumen micro flora. Whereas, intestinal and rumen bacterial diversity at wk 5 and 7 of age was not affected by inclusion of DDGS and CAFEX-DDGS in starter feed. The changes in intestinal microflora of DDGS and CAFEX-DDGS fed calves compared with control group showed that the effect of dietary treatments on post-ruminal availability of nutrients and microbial proteins. In conclusion, rumen bacterial population changes with the advancing age and the type of ingredients used in the diet. Further, research is required to identify the effect of feeding DDGS on growth of particular bacteria like methanogen and their impact on methane production and feed efficiency. Availability: Items available for loan: UVAS Library [Call number: 1595,T] (1).

9. Epidemiology, Serodiagnosis, Economic Losses and Control of Haemonchosis in Sheep and Goats

by Muhammad Faiz Qamar | Prof. Azhar Maqbool | Prof. Dr. Muhammad Akram Muneer | Prof. Dr. Makhdoom Abdul Jabbar | Prof. Dr. Muhammad Sarwar Khan.

Material type: book Book; Literary form: not fiction Publisher: 2008Dissertation note: CD not available. Availability: Items available for loan: UVAS Library [Call number: 2840-T] (1).



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