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1. A Study On Superovuation Protocol For The Development Of Embryo Transfer Technique In Mice

by Muhammad Ameen jamal | Dr. Amjad riaz | Dr. Aamir | Prof. Dr Mian abdul sattar.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1900,T] (1).

2. Effect Of Cholesterol Loaded Cyclodextrins (Clc) On Post-Thaw Quality In Canine Spermatozoa

by Junaid khan | Prof. Dr.Nasim ahmad | Dr. Asim khalid | Prof. Dr. Mian abdul sattar.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2005,T] (1).

3. Standardization Of Bovine Serum Albumin (Bsa) For Cryopreservation Of Beetal Buck Semen

by Muhammad Shahzad | Prof. Dr. Mian abdul sattar | Prof. Dr | Prof. Dr. Naseem ahmad.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2018,T] (1).

4. Effect Of Cholesterol-Loaded Cyclodextrin (Clc) Addition On Egg Yolk Ratio In Semen Extender And Post-Thaw Quality Of Buffalo Bull Sperm

by Mehboob Ahmed | Dr. Mushtaq Ahmad | Prof. Dr. Mian Abdul Sattar.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2085,T] (1).

5. Optimization Of Strontium Chloride For Parthenogenetic Activation Of Mouse Oocytes

by Arslan Mahmood Ahmad (2007-VA-67) | Dr. Amjad Riaz | Dr. Aqeel Javeed | Prof. Dr. Mian Abdul Sattar.

Material type: book Book; Format: print Publisher: 2014Dissertation note: There are two main methods by which activation can be performed: (i) physical methods and (ii) chemical methods. Physical methods include electrical stimulation, temperate and mechanical ways, whereas the chemical methods comprise of different artificial chemical agents, including strontium chloride, calcium ionophores, ethanol that promote to rise in intracellular Ca2+ oscillations, cycloheximide, that inhibit protein synthesis and 6-DMAP (6-dimethyl amino purine) which inhibit protein phosphorylation. The contribution of both maternal and paternal genomes is required for thedevelopment to full term of mammalian embryos. However, the percentage of parthenogeneticallyactivated embryos developing to blastocyst stage is lower as compared to normal fertilized embryos. (Renard et al. 1991).In mouse, strontium chloride has been successfully employed in manydifferent studies to induce artificial oocyte activation. The role of strontium to induce calcium oscillations appears to be more physiologically sound than alternativemethods of oocyte activation that produce a monotonic rise in calcium.Strontium chloride (SrCl2) is recognized as one of the most popular parthenogenetic agents for mouse oocytes activation and induces calcium oscillations leads to improved activation rate and blastocyst formation. (Locham-kaplan et al. 2003) (Satoshi et al. 2006). The diploid parthenogenetic oocytes have more developmental competence as compared to haploid form(Liu et al. 2002). A substancecytochalasin B (CB) prevents the release of the second polar body after activation of mammalian oocyte which results in diploid form of embryo (Fukui et al. 1992) and it may also contribute to prevent fragmentation and degradation of embryos ( Yi and Park 2005). Parthenogenetic oocyte activation technique is mainly used in cloning and is a key step for nuclear transfer for cloning. The technique is also useful for understanding of physiological mechanisms of fertilization and early embryonic development. Embryonic stem cells can be derived from fertilized embryos. The stem cells which are produced by parthenogenetic activation have the same totipotency and proliferation as formed by normal sperm-egg fertilization..( Ju 2008). Resultantly, parthenogenetic activation technology has become a target of reproductive biology. This technology can also be used to establish embryonic stem cell lines (Mizutani et al. 2004) and embryonic stems cells are the fundamental source in field of regenerative medicine; used to treat many diseases such as diabetes, beta thalassemia, heart infarction etc by providing patient specific replacement cells. Mouse is one of the most commonly animal models used for parthenogenetic activation. The other animals which have been used for parthenogenetic activation include rabbits, cattle, sheep, horses, monkeys and pigs. Parthenogenetic embryos are failed to develop to term, due to genomic imprinting, an epigenetic change of certain genes, depending on the parent of origin.(Uranga and Arechaga 1997). The studies pertaining to parthenogenetic activation technology for mouse oocytes is extremely limited at present (Mizutani et al. 2004). Availability: Items available for loan: UVAS Library [Call number: 2188,T] (1).

6. Comparative Effect Of Cidr Based Estrus Synchronization Protocol With Or Without Gnrh In Non-Descript Cows During Low Breeding Season

by Muhammad Bilal (2008-VA-152) | Prof. Dr. Mian Abdul Sattar | Dr. Muhammad Anwar | Dr. Muhammad Rizwan Yousuf | Dr. Muhammad Lateef.

Material type: book Book; Literary form: not fiction Publisher: 2015Dissertation note: The livestock sector occupies a peculiar position in the national plan of economic development in Pakistan. It subscribed approximately 55.9 percent to the agricultural value added and 11.8 percent to national GDP with a growth rate of 2.7 % in 2013-14 (Anonymous 2014). Dairying has become an important subsidiary source of income for thousands of rural families with an important role in generating earning opportunity. In Pakistan dairy sector is developing and commercializing at a rapid pace to meet increasing requirement of milk and other dairy products (Dongre et al. 2011). Pakistan owns renowned breeds of dairy buffaloes (Nili-Ravi and Kundi) and cattle (Sahiwal, Red Sindhi, Thari and many others). Cattle in Pakistan belong to genus Bos indicus. According to the latest livestock census (Anonymous 2006), out of 29.6 million cattle, 46% (13.6 million) have been described as non-descript. Non-descript cattle do not fall in any defined breeds of cattle. Milk production of non-descript cows is < 1000 lit per lactation in mountains area of NWEP pakistan (Khan and Usmani 2005). As non-descript breeds make up the largest group of cattle in Pakistan, there is a dire need to work on genetic improvement of these animals. Artificial insemination, the best tool for genetic up-gradation in dairy cattle is applied only in 11.1% cows in Pakistan (Anonymous 2006). The main hindrances are small sized scattered herds and lack of experienced technical manpower in the field. Estrus synchronization of a large number of animals and timed insemination can be used to overcome these hindrances .The technique may also help in 11 reducing a prolonged calving interval and postpartum anestrus and seasonality of breeding in these animals (Zafar et al. 2008). Estrus synchronization widely practiced in temperate dairy cattle in developed countries (Hansen and Arechiga 1999). Before launching a large scale estrus synchronization program in non-descript cattle, there is a need to assess the efficacy of various synchronization protocols in terms of estrus incidence, intensity and conception rate. Additionaly, distinct differences have also been reported between Bos taurus and Bos indicus in terms of estrus duration and intensity of expression of estrus sign (Mattoni and Ouedraogo 2000). The low estrus intensity and less duration of estrus signs of Bos Indicus are due to smaller diameter of follicle as compared to that of Bos Taurus (Bo et al. 2003). Developing successful methods for synchronizing estrus and ovulation in cattle has been a major research interest. Ultimately, the goal has been to achieve precise synchronization of ovulation so that cattle can be inseminated without regard to estrus detection. One method to increase conception rates is to use hormonal treatments in zebu breeds for synchronizing ovulation and for timed artificial insemination (TAI). Hormonal programs for synchronizing ovulation to control the follicular and luteal phases and estrus behavior have been used in Bos taurus cows and heifers(Castellanos et al. 1997), and Bos Indicus cows (Pinheiro et al. 1998). The intensity and duration of estrus behaviors during the estrous cycle is highly variable among individuals. More commonly animals are diagnosed to be in estrus based on the mounting or standing to be mounted, appearance of mucus discharge, and other physical activities (Van Eerdenburg et al. 2002). Scoring system were established on the basis of observed estrus signs and most of them have taken mounting and standing to be 12 mounted behavior as the most reliable signs to predict ovulation time in Bos taurus cows (Roelofs et al. 2005). However, it is now well acknowledged that the expression of estrus behavior change with breed of cows (Naidu and Babu Rao 2006). Fertility is an important parameter to assess the efficacy of estrus synchronization. Bos indicus cows after treatment with CIDR conception rate in adult cows 40% and in heifers 20% (Singh et al. 2006). CIDR may have ability to overcome the problems in field condition and increasing the reproductive efficiency by minimize the hindrance anestrus postpartum cows. In Pakistan research work have been done to evaluate the efficacy of CIDR for conception rate in indigenous cattle. Until now no study conducted on non- descript cattle. Therefore, it is hypothesized that CIDR+GnRH can provide better in vivo fertility compared to CIDR. Availability: Items available for loan: UVAS Library [Call number: 2248-T] (1).

7. Comparative Effect Of Alpha Lipoic Acid And Butylated Hydroxytoulene On Post Thaw Quality Of Buck Semen

by Muhammad Khurram Shahzad | Prof. Dr. Mian Abdul Sattar | Dr. Mushtaq Ahmad | Dr. Muhammad Yasin Tipu.

Material type: book Book; Literary form: not fiction Publisher: 2015Dissertation note: Amongst different livestock species, goats and sheep are the major source of livelihood for over a million livestock farmers in Pakistan. Total goat population in Pakistan is estimated to be 66.6 million. These animals are mostly kept by small holders for whom these are only source of their livelihood. Milk production from goats is 0.822 million tonnes while mutton production from both sheep and goats is 0.657 million tonnes (Anonymous 2014). Pakistani people mostly prefer the goat meat over sheep. All irrigated areas of Punjab including district Faisalabad, Sahiwal, Sargodha, Jhang, Jhelum, Lahore and Multan are the habitat of Makhi Cheeni Beetal goats. The color of its body coat is red spotted or golden brown with white patches. Its body is very well developed and compact. Males have long spiraled horns while females have shorter. It has roman nose with pendulous broad and long ears. It has long teats and well developed udder. Female weighs 37kg and males 46kg. Twins or triplets births are more than 50%. In 130 days of lactation period, there is 290 liters milk yield (Shah et al. 2001). Some breeds of goats especially dairy goats have more demand than the others and these bucks are not available everywhere. To cope with this situation artificial insemination techniques is necessary. Artificial insemination plays a great role in increasing the economics by spreading the superior genetics within a short period of time. Semen is processed by different methods but cryopreservation is considered to be the best method. Cryopreservation has been reported to compromise the quality of processed semen resulting in the loss of sperm motility, viability, in-vivo fertilizing ability, deterioration of plasma membrane and acrosomal integrity, apoptosis and damage of deoxyribonucleic acid (DNA) (Medeiros et al. 2002; Purdy 2006a). Sperm damage may occur due to various factors like osmotic stress, oxidative stress, low-temperature exposure and combination of different factors (Sarıözkan et al. 2009). Thawing of semen may also cause osmotic changes and the sperm quality is further decreased. It is generally accepted that sperm viability is reduced by as much as 50% during the process of semen cryopreservation (Watson, 2000). Extension of buck semen with egg yolk containing extender may be more injurious to sperms. This is due to the presence of coagulating enzymes of bulbourethral origin named as egg yolk coagulating enzymes (EYCE). EYCE decreases the tenacity of chilled or frozen semen (Roy, 1957). EYCE also catalyze the conversion of egg yolk lecithin into lsolecithin and fatty acid, thus sperm membrane become more fusogenic due to hydrolysis. So there is increase in chromatin decondensation and acrosomal reaction that is harmful for sperm (Leboeuf et al. 2000). Due to excess of poly unsaturated fatty acids (PUFA) in sperms, they are more susceptible to lipid peroxidation (Cassani et al. 2005). Lipid peroxidation of PUFA lead to production of reactive oxygen species (ROS) (Alvarez et al. 1995). Small amount of ROS are normally involved in capacitation, acrosmal reaction and ultimately fertilizing ability of sperms. But when the ROS are produced in excess Introduction 3 amount, these may compromise the enzymatic function and sperm fertility (Baumber et al. 2000). At 4-5 ºC the motility and plasma membrane integrity is decrease with the passage of time which ultimately leads to decrease in fertility. One of the cause of this decrease is production of ROS by the lipid peroxidation of spermatozoa’s membrane (Storey et al. 1998). Major decrease in sperm motility and fertility occur during phase transition from liquid crystalline to gel phase (Chakrabarty et al. 2007). Lipid peroxidation leads to irreversible loss in motility and damage to DNA of sperm (Maxwell et al. 1996). Motility of sperm is adversely effected with ROS, when the ROS harm the plasma membrane and acrosomal integrity which ultimately leads to fragmentation of DNA. Sperms have their own antioxidants system which include the glutathione (GSH) , GSH peroxides, superoxide dismutase, catalase and chelators of transferrin, lactoferrin and ceruplasmin (Agarwal et al. 2002). Normally the ROS production and scavenging are in equilibrium but during the semen preservation the excessive production of ROS (superoxide, hydroxyl, hydrogen peroxide, nitric oxide, peroxynitrile) with low level of scavenging system and antioxidants leads to oxidative stress. During the process of freezing and thawing the natural antioxidants systems are unable to stop lipid peroxidation. Therefore a powerful antioxidant system should be used to avoid the cryo-injuries and lipid peroxidation (Irvine 1996). Different antioxidants are being used i.e. fetuin (F), amino acid (AS), cysteine (CY) taurine, glutathione (GSH) glutathione peroxidase (GSH-PX), catalase (CAT), superoxide dismutase (SOD) glutamine, hyaluronan, trehalose, Alpha lipoic acid (ALA) and Butylated Hydroxytoulene (BHT) (Atessahin et al. 2008; Bucak et al. 2009; Taşdemir et al. 2014). Addition of antioxidants to semen extenders are considered to improve the quality of semen (Rao et al. 2013). ALA is a short chain fatty acid which act as an antioxidant in both aqueous and lipid environments, its therapeutic effects in other tissues like brain (Piotrowski et al. 2001), heart, kidneys and testicles has already been Introduction 4 discussed. It is called as universal antioxidant because of its effect in different parts of body. It is not only involve in scavenging the ROS but also activate the body antioxidants systems against ROS. ALA reduced to dithiol form called dihydrolipoic acid (DHLA) which is an excellent antioxidant (Handelman et al. 1994). ALA also regenerates vitamin C from reduced vitamin C in the presence of glutathione (GSH) which also enhance the antioxidant activity (Ibrahim et al. 2008). BHT, a phenolic lipophilic antioxidant that has antiviral activity, have the ability to relieve the cold shock in spermatozoa from several animal species. It stops the auto oxidation by converting the peroxide radical to hydroperoxide as it is also called as synthetic analogues of Vit E (Memon et al. 2011). BHT acts as a membrane lipid protectant which reduces the changes in permeability of sperm plasma membrane in cold shock (Graham et al. 1992). BHT minimizes the effect of cold shock on semen (Shoae et al. 2008), boar (Roca et al. 2004) and goat (Khalifa et al. 2008). Availability: Items available for loan: UVAS Library [Call number: 2254-T] (1).

8. Comparison Between Aspiration And Slicing Methods For Retrieval Of Oocytes In Bovine

by Muhammad Husnain (2008-VA-281) | Prof. Dr. Mian Abdul Sattar | Dr. Qaiser Shahzad | Dr. Amjad Riaz | Dr. Muhammad Avais.

Material type: book Book; Literary form: not fiction Publisher: 2015Dissertation note: Livestock contribution to agriculture stood at 55.9 percent while it contributes 11.8 percent to the national GDP during 2013-14. Buffalo, cattle, sheep and goat population in Pakistan is 34.6, 39.7, 29.1 and 66.6 million numbers during 2013-14. Total milk production from buffalo and cattle as major milk producing animals is 31,252 and 18,027 (000 tons) (Economic Survey of Pakistan 2013-14). Advanced biotechnologies coming from different areas of biological sciences exhibit great promise to enhance the efficiency of livestock production. From these technologies one such biotechnology is the use of in vitro maturation of follicular oocytes and in vitro fertilization for production of livestock embryos in laboratory. Proper oocytes recovery and their selection in the laboratory are of great importance for successful in vitro embryo production. Total one hundred and forty four ovaries (n=144) from cattle (72 ovaries) and buffalo (72 ovaries) were collected and 223 oocytes were retrieved from these ovaries. Average oocytes per ovary were 1.66 + 0.43 oocytes per ovary were obtained via aspiration and 1.89 + 0.00 average oocytes per ovary through slicing method from cattle ovaries. Average 1.55 ± 0.55 oocytes per ovary via aspiration and 1.53 ± 0.20 oocytes per ovary through slicing from buffalo ovaries. Overall grade-A oocytes were 28 (40) percent with aspiration in cattle and 25(36.76) through slicing method. In buffalo overall grade-A oocytes retrieval was obtained in percentage as 20 (44.44) and 26 (52) through aspiration and slicing methods respectively. Grade-B oocytes recovery obtained was in percentage as 23 (33.82) with slicing and 19 (31.67) through aspiration technique from cattle ovaries. Summary 26 Commonly used methods of recovery of oocytes from slaughterhouse animals are aspiration and slicing. Recovery rate of oocytes is different from slaughterhouse ovaries. Aspiration is the best method for retrieval of good quality oocytes from slaughterhouse bovine ovaries because it gave more good quality oocytes in less time than slicing method. In this study, it is found that weight of ovary and no. of follicles/ovary in cattle have strong correlation of 71% existed between weight of ovary and no. of follicles /ovary in buffalo was observed. Correlation between average number of follicles on ovary and weight /ovary was stronger in cattle. The more the number of follicles present on the ovaries and more weight of the ovary, the more will be the recovery of oocytes. In cattle average number of follicles was 10.09 ± 0.30 and when it was checked in buffalo, differed significantly and it was found as 7.16 ± 0.19 on an average per ovary. Likewise weight of buffalo in this study was differed significantly from cattle 4.04 ± 0.10 and 7.62 ± 0.15 respectively. It is suggested that oocytes retrieval should be done in buffalo using aspiration method to retrieve better quality oocytes. It is concluded that aspiration is the suitable method for retrieval of good quality oocytes from slaughterhouse buffalo ovaries because it gave more good quality oocytes in less time than slicing method. But both methods have minor difference between recovery rates but aspiration is more convenient than slicing and it yields more quality oocytes. It is also found that there is very strong correlation existed between average weight of ovary and number of follicles per ovary and the both parameters play a great help for more quality and quantity oocytes. Availability: Items available for loan: UVAS Library [Call number: 2279-T] (1).
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9. Effect Of L-Cysteine And Glutathione On Post Thaw Quality Of Sahiwal Bull Spermatozoa

by Farhan Younas (2007-VA-495) | Prof. Dr. Mian Abdul Sattar | Dr. Syed Murtaza Hasan Andrabi | Prof. Dr. Nasim Ahmad | Prof. Dr. Aneela Zameer Durrani.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2015Dissertation note: Freezing and thawing of semen leads to production of reactive oxygen species (ROS) due to plasma membrane lipid peroxidation. Because of this semen quality can be compromised. To overcome this problem, antioxidants have been used in cryopreservation medium. Glutathione and cysteine have thiol groups which penetrate into the cell and protect it from oxidative stress. In this study, effect of different concentrations of cysteine and glutathione on post thaw quality of Sahiwal bull spermatozoa was determined. Semen was collected with artificial vagina from five mature regular donor Sahiwal bulls kept at the Semen Production Unit Qadirabad, Sahiwal. Semen samples possessing >60% motility and >500x10 6 sperm/ml were included in study. After collection, semen samples from five bulls were pooled, divided into seven equal aliquots and kept at 37 ºC in water bath. After that dilution was done with Tris citric egg yolk extender having different concentrations of cysteine and glutathione as Con (0.0 mM), C1 (1.0 mM cystein), G1 (1.0 mM glutathione), CG0.5/1(0.5 mM Cysteine+1.0 mM glutathione), CG1/0.5 (1.0 mM cysteine+0.5 mM Glutathione), CG0.5/0.5 (0.5 mM cysteine+0.5 mM glutathione) and CG1/1 (1.0 mM cysteine+1.0 mM glutathione). Diluted samples were cooled to 4ºC in two hours and equilibrated for 4 hours at 4 o C. After that they were packaged into 0.5 ml French semen straws (20x10 6 sperm/straw). All semen straws were placed 4cm above liquid nitrogen surface in vapors for 10 minutes. Then, semen straws were plunged into liquid nitrogen for freezing and stored until post thaw analysis. The experiment was repeated for five times (replicates = 5). Four semen straws/treatment were thawed for 30 seconds in water bath at 37ºC and evaluated for visual motility, plasma membrane integrity (PMI), acrosome integrity, mitochondrial trans membrane potential and CASA motility parameters and kinematics. 42 Summary PMI in group CG0.5/0.5 was significantly higher (40.00±1.42 %) as compared to Con 26.67±0.80 (P<0.5). Plasma membrane integrity in groups CG1/1, CG0.5/1, G1 and C1 was significantly higher (36.00±1.88 %, 36.20±1.07 %, 33.60±1.21 % and 32.80±0.80 % respectively) as compared to Con (26.67±0.80 %) (P<0.05). There was no significant difference in C1 (32.80±0.80 %) and G1 (33.60±1.21 %) (P>0.05). In case of acrosome integrity, NAR value of group CG0.5/0.5 was significantly higher (71.40±1.08 %) as compared to Con (59.67±0.37 %) (P<0.05). All other groups also showed significant differences as compared to Con (P<0.05). CG0.5/0.5 also showed significantly higher NAR value (71.40±1.08 %) as compared to C1 (64.40±1.40 %) and G1 (67.60±2.07 %) (P<0.05). CG0.5/0.5 had significantly higher value (71.40±1.08 %) as compared to CG1/0.5 and CG1/1 (65.60±0.81 % and 68.80±0.97 % respectively) (P<0.05). CG0.5/0.5 had significantly higher subjective motility (54.00±1.88) as compared to Con (36.66±0.92) Mitochondrial transmembrane potential of CG0.5/0.5 was significantly higher (37.00±0.71 %) as compared to Con (25.33±1.28 %) (P<0.05). All the other treatment groups also had higher mitochondrial transmembrane potential as compared to Con (P<0.05). In groups of combination of cysteine and glutathione, CG0.5/0.5 showed significant difference (37.00±0.71 %) as compared to CG1/1 and CG1/0.5 (29.00±1.00 % and 33.80±0.86 %) respectively (P<0.05). CASA results showed that CG1/1 had significantly higher motility as compared to the control. But the percentage of progressive spermatozoa was significantly higher in CG0.5/0.5. VSL of group CG0.5/0.5 was significantly higher (53.33±2.90 %) as compared to Con (45.10±0.50 %). However, VSL, VCL, ALH and BCF did not vary significantly among groups. STR and LIN of group CG0.5/0.5 were significantly higher as compared to the control group. 43 Summary In conclusion, addition of cysteine and glutathione in tris citric egg yolk extender improved the post thaw quality of Sahiwal bull spermatozoa. In case of additive effect of cysteine and glutathione, CG0.5/0.5 showed higher plasma membrane integrity, acrosome integrity, mitochondrial transmembrane potential, progressive and rapid spermatozoa as compared to CG0.5/1, CG1/0.5 and CG1/1. 44 Availability: Items available for loan: UVAS Library [Call number: 2318-T] (1).

10. Effect Of Bio-Stimulation On Estrus Expression And Pregnancy Rate In Cidr Based Synchronization Protocol In Nili-Ravi Buffalo

by Abdul Waheed (2009-VA-133) | Dr. Aijaz ali Channa | Dr. Syed Murtaza Hassan Andrabi | Prof. Dr. Mian Abdul Sattar | Prof. Dr. Aftab Ahmad Anjum.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: Our water buffalo (Bubalus bubalis) has much potential for production of milk. But this animal has some problems regarding reproduction including delayed puberty, poor estrus behavior, silent heat, long postpartum period and low conception rate by artificial insemination. This leads to poor reproduction and hence great economic loss. Therefore, the requirement is to address these problems efficiently and formulate more effective techniques for improvements. Researchers have devised many estrus synchronization protocols (PGF2α, P4, GnRH, eCG, hCG etc.) that help bringing many animals in heat and hence improve the reproductive performance when fixed time artificial insemination is combined with them. But these protocols give inconsistent results when they are applied on buffaloes making it necessary to improve the techniques. This study was planned on the hypothesis that presence of bull (bio-stimulation), at the time of synchronization, may play an important role in enhancement of estrus intensity and fertility rate in Nili-Ravi buffaloes. Seventy one adult buffaloes were randomly selected from different areas of field conditions and LRS (NARC) and subjected to CIDR based heat synchronization in combination of either bio-stimulation or non-stimulation. The animals were observed for behavioral estrus signs twice a day starting after 12 hours of CIDR removal till 96 hours. Pregnancy diagnosis was done by rectal palpation 60 days post CIDR removal. Estrus response and pregnancy rate were analyzed by Chi-square test using MINITAB version 15. Estrus signs and total estrus intensity were compared by Mann Whitney U test. Difference was considered significant at probability level of (P < 0.05). In peri-urban areas, more animals from bio-stimulated group showed better behavioral estrus signs, more total intensity score and significantly higher pregnancy rate as compared to nonSUMMARY 63 stimulated group of animals. At LRS (NARC), more animals from non-stimulated group were found in behavioral estrus but intensity of heat signs was high in bio-stimulated animals. Pregnancy rate was also higher in non-stimulated animals but the difference was not significant. Overall, in this study, we got higher pregnancy rate in bio-stimulated animals than non-stimulated group which indicates a positive response of bull stimulation on reproductive performance of Nili- Ravi buffaloes who were synchronized with CIDR based estrus synchronization protocol. Availability: Items available for loan: UVAS Library [Call number: 2469-T] (1).

11. Comparison Of Commercial Triladyl Extender With A Tris-Citric-Egg-Yolk (TCEY) Extender On Post-Thaw Semen Quality Of Nili Ravi Buffalo

by Muhammad Asad Ullah Khan | Prof. Dr. Mian Abdul Sattar | Prof. Dr. Nasim Ahmad | Prof. Dr. Mansur ud Din Ahmad.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: Cryopreservation of semen is the most important step for its usage in artificial insemination. Freezing of semen leads to a remarkable reduction in post-thaw semen quality. Therefore, selection of a better semen extender has always been considered priority that could serve as a good cryoprotectant.. Our semen production units (SPUs) have been using Tris based egg yolk semen extender since long time. Some modern SPUs like CEBG are using commercially available semen extenders for better post-thaw semen quality. After collection pooled semen divided into two equal aliquots in separate sterilized test tubes and kept in water bath at 37 ºC. Semen was diluted with each of extender (TCEY and Triladyl) on the basis of sperm concentration (40x106sperm/ ml). Diluted semen was placed bottles and placed in safety cabinet cooled to 4 ºC over and equilibrated for 4 hrs. After equilibration semen was filled in 0.5 ml French straws (20x106sperm/ 0.5 ml). All semen straws placed in automatic freezer 4cm above liquid nitrogen surface in vapors for 10 minutes. Liquid Nitrogen vapors used in automatic programmable freezer to reduce temperature from 4 ºC to -180 ºC and then plunged into liquid nitrogen -196 ºC for freezing and was stored until analyzed. The experiment was repeated for seven times (replicates = 07) CASA sperm motility parameter and kinematics were analyzed at Center of Excellence for Bovine Genetics (CEBG) Renala khurd District Okara. For further analysis frozen semen straws were brought to the Department of Theriogenology UVAS, Lahore. Effects of Triladyl and TCEY on post-thaw semen quality of the Nili Ravi buffalo semen were compared. Summary 54 In Triladyl group, significantly (P<0.05) higher post-thaw motility (PTM %), Plasma membrane integrity (PMI, %),) DNA integrity (%), Live percentage was found. However, no significant (P<0.05) difference was found regarding NAR results between both groups. Sperm abnormalities were found significantly lower in Triladyl group as compared to TCEY group. In overall assessment regarding and post-thaw CASA motility parameters, CASA motility, (PROG %), rapid (RAP %), medium (MED%), and slow (Slow, %) and sperm motility kinematics (VAP μm/sec), (VSL μm/sec), (VCL μm/sec), (ALH μm), (BCF HZ), (STR%) and (LIN%) Triladyl was found better than TCEY. This was concluded that use of commercial semen extender Triladyl resulted in significantly better post-thaw semen quality as compared to Tris citric egg yolk (TCEY) extender. Availability: Items available for loan: UVAS Library [Call number: 2581-T] (1).

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