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Effect Of "In Process Quality Control "Factors On Efficacy Of Bird Flu Vaccine

By: Saeed Khan | Prof.Dr.Khushi Muhammad.
Contributor(s): Prof.DR.Irshad Hussain | Prof.Dr.Muham | Faculty of Veterinary Sciences.
Material type: materialTypeLabelBookPublisher: 2008Subject(s): Department of MicrobiologyDDC classification: 1013,T Dissertation note: Bird Flu virus was recovered from lungs, trachea, spleen and fecal contents of the infected birds in 10 days old chicken embryos. HA activity and biological titer of the virus improved by serial passages in the 10 days old chicken embryos. This could be due to high rate of mutation of the Bird Flu virus with its successive passages. Formaldehyde and binary ethylenimine (BET) effectively inactivated the virus. However formaldehyde inactivated virus showed mitigation in HA activity during storage. The BET 5mM inactivate the virus with in 16 hours of incubation at ambient temperature (25°C) or 37°C. It has minimal detrimental effect on the HA activity of the virus, even during storage at refrigeration temperature. Bird Flu virus vaccines without adjuvant induced poor antibody response in the vaccinated broilers. The vaccine containing aluminium hydroxide gel induced antibody response that reached at peak level on 1 8 days post priming and decline thereafter. The vaccine containing montanide (oil based vaccine) increased (90.5 GMT) up to 42 days of age. Boosting of the birds primed with gel based Bird Flu virus vaccine improved the production of antibody titer, while boosting of birds primed with oil based Bird Flu vaccine showed undetectable effect. This was due to increasing trend of antibody titer in oil based primed birds. Montanide based vaccines are therefore recommended for broiler, layers and breeders in high risk area of the disease. Vaccines containing decreased infectivity titer induced decrease antibody titer in the vaccinated broilers. Bird Flu virus improved its HA activity and infectivity titer with serial passages in 10 days old chicken embryos. It is worth mentioning that serial passages of the virus tremendously decreased its antigenicity. It is therefore recommended to prepare commercial vaccine fom passage number 1-4, for effective immuno-prophylaxis. The Bird Flu virus (H5N1) mutates very rapidly every time it passes through chicken embryos. It is therefore suggested to grow the virus at least in bio-safety level-ll plus (BSL-II +) laboratories. On account of it high rate of mutation and risk of human health hazards it is suggested that Bird Flu virus (H5N1) may not be used in vaccine production, however other serotypes containing H5 and N antigen other than N1 may be used for production of commercial vaccine.
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Veterinary Science 1013,T (Browse shelf) Available 1013,T
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Bird Flu virus was recovered from lungs, trachea, spleen and fecal contents of the infected birds in 10 days old chicken embryos. HA activity and biological titer of the virus improved by serial passages in the 10 days old chicken embryos. This could be due to high rate of mutation of the Bird Flu virus with its successive passages. Formaldehyde and binary ethylenimine (BET) effectively inactivated the virus. However formaldehyde inactivated virus showed mitigation in HA activity during storage. The BET 5mM inactivate the virus with in 16 hours of incubation at ambient temperature (25°C) or 37°C. It has minimal detrimental effect on the HA activity of the virus, even during storage at refrigeration temperature.

Bird Flu virus vaccines without adjuvant induced poor antibody response in the vaccinated broilers. The vaccine containing aluminium hydroxide gel induced antibody response that reached at peak level on 1 8 days post priming and decline thereafter. The vaccine containing montanide (oil based vaccine) increased (90.5 GMT) up to 42 days of age. Boosting of the birds primed with gel based Bird Flu virus vaccine improved the production of antibody titer, while boosting of birds primed with oil based Bird Flu vaccine showed undetectable effect. This was due to increasing trend of antibody titer in oil based primed birds. Montanide based vaccines are therefore recommended for broiler, layers and breeders in high risk area of the disease.

Vaccines containing decreased infectivity titer induced decrease antibody titer in the vaccinated broilers. Bird Flu virus improved its HA activity and infectivity titer with serial passages in 10 days old chicken embryos. It is worth mentioning that serial passages of the virus tremendously decreased its antigenicity. It is therefore recommended to prepare commercial vaccine fom passage number 1-4, for effective immuno-prophylaxis.

The Bird Flu virus (H5N1) mutates very rapidly every time it passes through chicken embryos. It is therefore suggested to grow the virus at least in bio-safety level-ll plus (BSL-II +) laboratories.

On account of it high rate of mutation and risk of human health hazards it is suggested that Bird Flu virus (H5N1) may not be used in vaccine production, however other serotypes containing H5 and N antigen other than N1 may be used for production of commercial vaccine.

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