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1. Sero-Prevalence Of Brucellosis In Buffaloes And Cattle Of Swat Valley And Government Livestock Farms,Nwfp

by Azhar Khan | Prof.Dr.Masood Rabbani | Prof.Dr.Azhar | Prof.Dr.Khushi Muhammad | Faculty of Veterinary Sciences.

Material type: book Book; Format: print Publisher: 2008Dissertation note: Brucellosis is an infectious zoonotic disease that is associated with chronic debilitating infections in humans and reproductive failure in domestic animals (Corbell, 1997). The sero-prevalence of brucellosis in buffaloes and cattle were undertaken by collecting samples from animals brought to various slaughterhouses and Private and Govt. farms in Swat valley and Peshawar division by screening through i-LLISA, MRT and RBPT. Out of 850 samples, 600 sera, 200 milk samples were collected along with 50 samples of slaughterhouse worker, butcher and veterinarian for this study. All the serum samples tested through RBPT and I-ELISA showed the overall prevalence 3.67% and 4.33% in the cattle and buffaloes population respectively while the combined prevalence in the cattle, buffaloes and human population through RBPT was 3.38 % and through i-ELISA was 4%. The high rate of brucellosis was recorded through RBPT and i-ELISA in buffaloes ( 4.75%,5.5%) while 0.0% prevalence in male buffaloes through RBPT and iELISA, where as in female buffaloes it was 4.85% through RBPT and through i-ELISA was 5.626%. The comparatively low rate (1.5%) of brucellosis was noted in cattle through the RBPT and 2% through i-ELISA while in female cattle it was 1.587% through the RBPT and through i-ELISA 2.12% with 0.0% in males. Among the serum samples (30) of buffalo and cattle having reproductive disorder were tested through the same tests which showed overall prevalence 16.6%. The prevalence at Cattle Breeding and Dairy farm 1-larichand and Livestock Research and Development Farm (Surrezai was 0.0% through Milk Ring Test and i-ELISA. Also cattle milk samples (110) from private farms in swat valley showed prevalence through Milk Ring Test 0.9% and through i-ELISA prevalence was noted to be 1 .82%. As 50 human serum samples were tested through RBPT and i-ELISA but none of these samples were positive showed that the prevalence of brucellosis in human being is very low, The comparison of RBPT, MRT and i-ELISA (milk and serum) was also analyzed statistically by z-test, the data revealed insignificant results. Availability: Items available for loan: UVAS Library [Call number: 1012,T] (1).

2. Study On Molecular Diagnosis Of Canine Distemper Virus

by Muhammad Zubair Shabbir | Prof.Dr.Masood Rabbani | Prof.Dr.Khushi Muhammad | Prof.dr.Zafar | Faculty of Veterinary Sciences.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2008Dissertation note: Samples from fourty five dogs were submitted to the University diagnostic Laboratory, University of Veterinary and Animal Sciences, Lahore from January, 2007 to January 2008 for diagnosis of CDV infection. These dogs presented to referring veterinarians with clinical signs suspicious of CDV infection. Hematological examination (lymphocyte count) was carried out using K-EDTA anti-coagulant added whole blood and RT-PCR tests were performed using biological fluid samples that include plasma, nasal and conjunctival swabs. Only distemper positive dogs by RT-PCR were followed up for subsequent lymphocyte count and prognosis of distemper infection. All the distemper positive dogs were lymphopenic but the degree of severity was variable as the samples were collected from dogs of different ages and phase of the disease. The study revealed that lymphopenia can be used to support presumptive clinical diagnosis but required laboratory procedure for confirmation and animal regain its normal value with the passage of time subjected to recovery. During followed up, two dogs were found to be dead because of CDV infection mixed with secondary bacterial infection in which one exhibited the nervous sign like teeth grinding, ataxia, convulsions and in coordination in body movements. Only ten (22.22%) samples were found positive by RT-PCR using plasma, nasal and conjunctival swabs. CDV RNA was detected in 60% of plasma samples, 70% of nasal and 100% of conjunctival swab sample from lymphopenic dogs whereas the percentage was 13.33, 15,55, and 22.22 from a total of 45 samples. No amplicon of expected length was obtained from normal healthy dogs. On comparison of different fluid samples, the sensitivity of conjunctival swab was found to be highly significant followed by nasal swab and plasma. In conclusion, Lymphopenia is the suggestive of clinical infection of dogs with canine distemper virus ad can help in presumptive diagnosis. It is not necessary that all lymphopenic dogs are distemper posit it requires further laboratory confirmtion. In this context, RT-PCR is test of choice with samples including conjunctival swabs and plasma. Availability: Items available for loan: UVAS Library [Call number: 1034,T] (1).

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