Role Of Single Injection Of Prostaglandin F2 Alpha On Breeding Efficiency Of Buffaloes
Material type: Book ; Format:
; Nature of contents: ; Literary form: Publisher: 1998 Dissertation note: In the present study, a total of twenty Nili-Ravi buffaloes were divided into two equal groups. In group A ten buffaloes were administered with prostagladin F2 alpha (Lutalyse, Upjohn), 2 hours after calving. In group B, ten buffaloes were not given any treatment and designated as control. The reproductive organs of each experimental buffalo were rectally palpated on day 14 and day 21 postpartum. After that twice a week rectal palpation was carried out until the first postpartum oestrus.
The results of present study revealed that cervical and uterine involution was completed significantly (P < 0.05) earlier in group A as compared to group B (28.90± 1.79 and 35.40±3.95 days). There was no significant difference in the diameter of cervix, gravid and nongravid uterine horn at day 14 postpartum. A significant difference between the groups was obtained on days 21, 25 and 28 postpartum in the diameter of cervix and gravid horn. The corpus luteum (CL) of pregnancy regressed very rapidly following calving. The overall period required for complete regression of corpus luteum of pregnancy was (19.20±4.87 days) in treated group and (18.40±6.07 days) in control groups. The difference was significant.
Follicular activity resumed independently of uterine involution. It was, however, delayed slightly by the retained corpus luteum of pregnancy. The mean postpartum interval of initial follicular development was 21.20±5.71 days in treated and 28.20±8.75 days in control groups, respectively. The difference was statistically significant (P <0.05).
Postpartum oestrus interval was shortened in treated group (79.50±19.83 days) as compared to control group (103.0± 17.45 days) and the difference was significant (P<0.05). So it seems beneficial to administer prostaglandin F2 alpha in postpartum buffaloes to reduce the period for uterine involution and enhance the subsequent reproductive performance.
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Effect Of Trehalose And L-Cysteine On Post Thaw Semen Quality, Antioxidant Enzyme Activity And Fertility In Nili Ravi Buffalo Bulls
Material type: Book ; Literary form:
Publisher: 2016 Dissertation note: Addition of various antioxidants in semen extender is one of the vital strategies being applied in reproductive biology for attaining functionally/structurally integral sperms and hence an appropriate conception rate. It is well established that chilling of buffalo semen results in decreased semen quality which is highly associated with decreased antioxidant activity and higher ROS production. Furthermore, buffalo bull spermatozoa are more susceptible to oxidative damage as compared to cattle bull spermatozoa. It is believed that this difference is due to higher contents of polyunsaturated phospholipids present in plasma membrane of buffalo bull spermatozoa. Freezing process accelerates the production of ROS molecules which may decrease the viability of buffalo bull spermatozoa during storage. Therefore, supplementation of antioxidants in semen extender is required to decrease the ROS-mediated damages to buffalo spermatozoa. The present study had, hence, been designed to monitor the effects of trehalose and L-Cysteine on the semen quality, antioxidant enzyme activity and fertility of Nili Ravi Buffalo bulls.
Semen samples (n= 20) from 4 buffalo bulls were diluted in Tris-citric acid based extender having different concentrations of trehalose (0.0, 15, 30, 45, and 60 mM) and frozen in French straws. At post dilution, profile of sperm catalase (U/mL) was higher (P < 0.05) in extenders containing 15, 30 and 45 mM of trehalose as compared to control. While profiles of superoxide dismutase (U/mL) and total glutathione (μM) were higher (P < 0.05) in extenders containing 15 and 30 mM of trehalose as compared to control. At pre freezing, sperm catalase, superoxide dismutase and total glutathione profiles were higher (P < 0.05) in all the treatment groups as compared to control. At post thawing, the profiles of catalase and total glutathione
were higher (P < 0.05) in extender containing 30 mM trehalose as compared to other treatment groups and control. Whereas, profile of superoxide dismutase was higher (P < 0.05) in extenders containing 30, 45 and 60 mM of trehalose as compared to control and 15 mM group. Post thaw total sperm motility (%) was higher (P < 0.05) in extender containing 30 mM trehalose as compared to control and 15 and 60 mM groups. While sperm progressive motility (%), rapid velocity (%), average path velocity (μm/s), straight linear velocity (μm/s), curvilinear velocity (μm/s), plasma membrane (structural and functional, %), acrosome (%) and DNA (%) integrity were higher (P < 0.05) in extender containing 30 mM trehalose as compared to other treatment groups and control. The fertility rates (61% vs. 43%) were higher (P < 0.05) in buffaloes inseminated with semen doses cryopreserved in extender containing 30 mM of trehalose than the control. It is concluded that addition of 30 mM trehalose in extender improves the semen antioxidant enzymes activity, post thaw quality, and fertility in Nili Ravi buffaloes.
Similarly Semen samples from 4 buffalo bulls were diluted in Tris-citric acid based extender having different concentrations of L-cysteine (0.0, 0.5, 1.0, 2.0, and 3.0 mM) and frozen into 0.5 ml French straws. The antioxidative enzymes [catalase, super oxide dismutase and total glutathione (peroxidase/reductase)] were significantly higher (P< 0.05) at pre freezing and post thawing in extender containing 2.0 mM L-Cysteine as compared to other groups. Post thaw total motility (%), progressive motility (%), rapid velocity (%), average path velocity(μm s-1), straight line velocity (μm s-1), curvilinear velocity (μm s-1), beat cross frequency (Hz), viable sperm with intact plasmalemma (%), acrosome and DNA integrity (%) were higher with addition of 2.0 mM L-cysteine as compared to other groups (P < 0.05). The fertility rates (59 vs. 43%) were higher (P < 0.05) in buffaloes inseminated with doses containing 2.0 mM of L-cysteine than the control. In conclusion, addition of 2.0 mM L-cysteine in extender improved the
antioxidant enzymes profile, post thaw quality and in vivo fertility of Nili Ravi buffalo bull spermatozoa.
It was concluded that addition of 30mM Trehalose and 2.0mM L-Cysteine in semen extender has significantly improved semen antioxidant enzymes activity, post thaw quality and fertility in Nili Ravi buffaloes.
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Effect Of Age On Lipid Peroxidation Of Fresh And Frozen-Thawed Semen Of Nili-Ravi Buffalo Bulls
Material type: Book ; Literary form:
Publisher: 2016 Dissertation note: Buffalo spermatozoa are rich in polyunsaturated fatty acids and prone to lipid peroxidation. Malondialdehyde (MDA) is a byproduct of lipid peroxidation and causes irreversible damage to sperm structure and function. In buffalo, blood plasma MDA level increases with age. Therefore, we hypothesized that MDA level in buffalo bull semen will increase with age and will affect the semen quality. The objective of the study was to compare MDA level and quality of fresh and frozen-thawed semen in aged vs. young Nili-Ravi buffalo bulls. Single ejaculate was collected on weekly basis for four weeks from aged (13.6±1.0 years; n=3) and young (3.4±0.3 years; n=3) Nili-Ravi buffalo bulls. MDA level was estimated through thiobarbituric acid assay (TBA) in fresh and frozen-thawed semen. The quality of fresh and frozen-thawed semen was estimated through sperm motility, viability, DNA and acrosome integrity. MDA level (nmol/ml) did not differ (P>0.05) between aged vs. young bulls in fresh (2.3±0.2 vs. 2.9±0.7) and frozen-thawed (53.1±2.8 vs. 48.4±2.6) semen, respectively. In fresh semen, sperm motility and concentration did not differ (P>0.05) in aged vs. young bulls; however, the volume of fresh semen increased (P<0.05), while sperm viability and DNA integrity decreased (P<0.05) in aged vs. young bulls. In frozen-thawed semen, sperm motility, viability, and DNA integrity decreased (P<0.05) in aged vs. young bulls. In frozen-thawed vs. fresh semen, MDA level (nmol/ml) increased within young (48.4±2.6 vs. 2.3±0.2) and aged bulls (53.1±2.8 vs. 2.9±0.7), while motility and viability decreased (P<0.05) within the age groups. In conclusion, 1) lipid peroxidation (MDA) does not increase due to age in buffalo bull semen, and 2) freezing causes increase in lipid peroxidation irrespective of age and deteriorates semen quality of Nili-Ravi bulls.
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