351.
Impact Of Different Protein Sources With Digestible Lysine, Methionine + Cysteine Ratios On Production Efficiency, Carcass Characteristics, Gut Development And Total Tract Protein Digestibility In Broilers
by Ghulam Mustafa (2009-VA-471) | Prof. Dr. Anujum Khalique | Prof. Dr. Saima | Prof. Dr. Athar Mehmud.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Due to price volatility of highly digestible but expensive protein sources like SBM future poultry diets will be formulated by using cheap and less digestible protein sources including CM and SFM due to price volatility. Undigested protein in hind gut subjected to microbial fermentation, resulted into formation of nitrogenous products, which are not only harmful to birds, but also excreted in litter bedding and generate ammonia, nitrous acid and nitrous oxide, the latter is powerful greenhouse gas with an effect about 300 times that of carbon dioxide. The potential solution to this problem is supplementation of synthetic amino acids (nearly 99 % digestible) in low nutrient density (crude protein) formulated by using cheap and less digestible protein sources. Therefore, objectives of current experiment were to determine the impact of digestible Lys, Methionine + Cysteine (M + C) ratios from different protein sources on production efficiency, carcass characteristics, gut development and total tract digestibility of protein in broilers. In total, mixed sex Ross 308 day old broilers were randomly assigned to 9 dietary treatments with replication of 6 replicates per treatment and 8 birds in each replicate. 1) Control corn soy based diet, 2) Canola meal (CM) based high crude protein ( 21% during starter phase and 19% during grower phase) diet with ideal protein ratio (IPR), 3) CM based high protein diet with 0.05 supplemented ( M + C ), 4) CM based low protein (19% during starter phase and 17% during grower phase) diet with IPR, 5) CM based low protein diet with 0.05 supplemented ( M + C ), 6) Sunflower meal (SFM) based high protein diet with IPR, 7) SFM based high protein diet with 0.05% supplemented (M + C), 8) SFM based low protein diet with IPR and 9) SFM based low protein diet with 0.05 % supplemented (M + C). All diets were fed as mash form in two phases (Starter 1-21 days and Grower 22-42 days). Feed and water were provided ad libitum throughout the experimental period. Celite @ 2% were used an inert marker for digestibility
CHAPTER 6
SUMMARY
SUMMARY
42
study. Average body weights, FCR, mortality rate were recorded from each replicate for the estimation of production efficiency factor. At 42 day, fresh excreta were collected from each replicate and 2 birds from each replicate were randomly selected and slaughtered for carcass and gut measurements. Crude protein and acid insoluble ash (AIA) in feed and excreta samples were determined. The results of the present study indicate that the protein sources showed non-significant difference on production efficiency and gut development, however, significant difference was observed on carcass characteristics and total tract digestibility. The birds fed higher CP level showed significant difference on production efficiency, carcass characteristics and total tract digestibility of protein. Amino acid supplementation showed significant difference on production efficiency, carcass characteristics, gut development and total tract digestibility of protein. In this study, CM and SFM showed poor performance regarding production efficiency, protein digestibility, gut development and carcass characteristics compared with SBM. However, the supplementation of Meth had improved the performance. It can be concluded that Meth supplementation may improve the overall performance in broilers fed poorly digestible protein source (CM and SFM).
Further research is needed to evaluate the different strategies to improve the digestibility of poorly digestible protein sources including guar meal. Threonine also has an important role in gut development, so there is a need to study the effects of supplementation of threonine above the recommended levels in broilers fed poorly digestible protein sources. There is a need to evaluate the other protein sources that may replace SBM in broilers. Availability: Items available for loan: UVAS Library [Call number: 2586-T] (1).
352.
Effect of Super Doses of Phytase from Two Different Sources on Growth Performance, Phosphorus Utilization and Carcass Characteristics of Broilers
by Faisal Mushtaq (2009-VA-369) | Prof. Dr. Saima | Prof. Dr. Athar Mahmud.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Cereal grains contain almost two-third of the P that is present in phytate form. Phytate possesses anti-nutritive properties as it binds other nutrients like amino acids and carbohydrates. Birds are unable to use P bound with phytate complex. The phytate P is excreted into the environment causing pollution. To compensate requirements expensive inorganic P sources like DCP and rock phosphate are used in poultry diets, which ultimately increase the ration cost. Exogenous phytase is capable of degrading phytate into lower esters having less chelating capacity. Commercially phytase enzymes are being added in the poultry diets to hydrolyze the phytate P that can replace the expensive inorganic P addition. In the industry, phytase is being added in the poultry diets at 500 FTU/kg. Use of phytase at this rate still leaves some gap. Keeping this thing in view present study was planned to use the super dose of phytase from two different sources (Buttiauxella and E.coli) to minimize the anti-nutritive aspects of phytate and use the phytate P at maximum. For this purpose 35 day feeding trial was conducted to evaluate the effect of super dosing of phytate from two different origins on growth performance, phosphorus utilization and carcass characteristics of broilers. For this in total, 672 one-day old broiler chicks were randomly selected& assigned to 8 dietary treatments, each having 3 replicates of 28 birds. One group was reared on positive control diet formulated according to the Hubbard strain recommended guides with adequate P without any phytase addition. Second group was offered NC diet (deficient in Av.P). While other six diets were formulated with three levels (500, 1000, 1500 FTU/kg) of E.coli and Buttiauxella origin supplemented in NC diet. The FI, BWG and FCR were recorded at the end of each week. At the end of experiment n=3 birds per replicate were selected randomly, weighed and slaughtered to estimate plasma P, ileal digestibility of P, tibia ash, organ weight and dressing percentage. The data collected were analyzed using analysis of variance (ANOVA)
SUMMARY
41
technique in a Completely Randomized Design (CRD) and means were be compared through Duncan’s multiple range test (DMR) (Duncan 1955) by using SAS (version 9.1; SAS Inst. Inc., Cary, NC).
Results of the experiment revealed that FI, BWG and FCR was higher 48%, 11% and 64.5% respectively in phytase supplemented diet best treatment T8, when compared to the NC. Phytase supplementation had no effect on organ weights. Organ weight was higher in diet low in NPP. Phytase supplementation had no effect on gizzard weight even at higher doses. Similarly phytase supplementation had positive effects on bone mineralization. Phytase addition in the diets improved tibia ash, Ca and P% at all levels (P <0.01). Plasma P level was significantly increased at higher level of phytase. The AIDC of P was linearly increased by phytase supplementation. Considering the economics phytase supplemented diets at higher level 1500 FTU/kg were most economical when cost of feed calculated to attain 1kg of live weight.
From this experiment it can be concluded that phytase supplementation at higher dose than the standard ensures the maximum break down of phytate P and reduce its anti-nutritive aspects apart from the source of phytase used in the experiment. Dietary inclusion of phytase at super dose economically generates the bio-available P and reduces the P load in the environment improve growth performance, and bone mineralization.
To replace the highly expensive phosphorus sources super dose of phytase can be used instead of standard dose. It can replace the expensive source of inorganic P and economically can generate more profit. Optimum level of phytase in the diet need to be reevaluated to replace inorganic P to some extent, minimize the anti-nutritive aspects of phytate and reduce P load in the environment. Availability: Items available for loan: UVAS Library [Call number: 2607-T] (1).
353.
Effect Of Methionine And Mannan Oligosaccharides Supplementation On Growth Performance, Carcass Characteristics, Immunity And Selected Blood Metabolites In Broiler Chicken Under Conditions Of High Ambient Temperature And Humidity
by Asif Mahmood (2009-VA-379) | Dr. Muhammad Afzal Rashid | Prof. Dr. Talat Naseer Pasha | Prof. Dr. Athar Mahmud.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Poultry sector is one of the most organized and vibrant segment of agriculture sector in Pakistan and is claimed to be among the largest industries of Pakistan with over more than 200 billion rupees investment and offering employment directly or indirectly to 1.5 million people. Broilers exposed to high environmental temperature and humidity exhibit several physiological and performance related setbacks. Heat stress cause high mortality, poor FCR, reduced feed consumption, body weight gain, and visceral development. Heat stress has been linked to a decrease in intestinal immune activity and to impaired intestinal morphology following pathogenic bacteria invasion of the body through the intestinal epithelium. Nutritional manipulations with its low cost are preferred to alleviate the negative effects of heat stress on performance. Nutritional strategies such as the addition of prebiotics, some amino acids e.g. methionine are reported to be beneficial in alleviating heat stress. As a nutritional intervention, synthetic methionine added to the diet has been explored as method of ameliorating the effects of ROS associated with high environmental temperature. Keeping above in view, the present study has been planned to evaluate the effect of supplementing methionine and MOS on the growth performance, carcass characteristics and immune status against ND under field conditions. In this experiment a total of n=200 day old chicks were procured from a commercial hatchery and randomly assigned to dietary treatments with n=40 chicks per treatment. Each treatment was further divided into four replicates with n=10 birds/replicate. Dietary treatments were : 1) negative control (NC) without any supplementation, 2) positive control (PC) containing antibiotic, 3) MOS supplemented (MOS) containing 800 and 400 g/ton of MOS in the starter and grower, respectively, 4) High Methionine supplemented treatment (HM) containing Methionine 1% of the diet in both starter and grower feeds, 5) Methionine and MOS supplemented (HM+MOS) treatment containing high methionine
SUMMARY
42
(1% in starter and grower) along with 800 and 400 g/ton of MOS in the starter and grower, respectively. The temperature and humidity were recorded daily at 8:00, 14:00 and 18:00 h and means were calculated to determine the daily changes in temperature humidity index. Feed intake, body weight and FCR were recorded at the end of each week. At the end of experiment n=3 birds per replicate were selected, weighed and slaughtered. At the time of slaughtering blood samples were collected in evacuated tubes. Serum was harvested after centrifugation, dived into aliquots and stored at –20 oC until further analysis. Serum samples were used to determine antibody titer against ND. At the time of slaughtering organ weighed and jejunal sample were collected to determine the gut morphology. The data collected were analyzed using analysis of variance (ANOVA) technique in a Completely Randomized Design (CRD). Results were declared statistically significant at P < 0.05 and compared using Tukey, s multiple comparison test. The statistical analysis were carried out using SAS 9.1 (SAS Institute, 2006). Live BW was higher (P<0.05) in HM+MOS compared to NC and HM. Feed intake was higher (P<0.05) in HM+MOS and PC than those in NC. However, FCR was lowest in HM+MOS compared to NC and PC birds. Relative organ weights were similar (P>0.05) among treatments; however, bursa of fabricius was heavier in MOS compared to the NC and HM+MOS. Villus height and villus to crypt ratio was highest (P<0.05) in HM, than the NC treatment birds CONCLUSIONS In conclusion, supplementation of methionine at 1% of diet and MOS improved the weight gain, feed intake and feed conversion ratio in birds reared under conditions of high temperature and humidity. Gut morphology of the bird also improved by supplementation of Methionine and MOS in the broiler diet.
SUMMARY
43
SUGGESTION AND RECOMMENDATION
Further research is needed to re-determine the recommended levels of methionine in the diet when bird are reared under the conditions of high temperature and humidity. Availability: Items available for loan: UVAS Library [Call number: 2606-T] (1).
354.
Protease Supplementation Influences Growth Performance, Gut Health And Ileal Digestibility Of Protein In Broilers Fed Low Protein Diets
by Shahzad Ahmad (2008-VA-240) | Prof. Dr. Talat Naseer Pasha | Prof. Dr. Saima | Prof. Dr. Athar Mahmud.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Proteases are protein-digesting enzymes that are used to break down the proteins of various plant materials and proteinaceous anti-nutrients in plant protein feedstuffs. The use of exogenous proteases can help in the reduction of the protein contents of a given feed by improving the digestibility and availability of the protein thereby reducing the cost of feed contributed by the protein feedstuff. It will also help to reduce the nitrogen excretion by the poultry and will contribute to mitigate the climate change.
In the present study the protease from a bacillus lachniformis spp was supplemented to evaluate the effect of enzyme on protein digestibility, gut health and birds performance. For this three experimental diets with 21, 19 and 17 % CP (HP, MP and LP) with six treatments with and without exogenous protease were used in this trial to evaluate the effect of protease. Each treatment was replicated to 6 pens and 10 birds were allocated to each pen. Parameters studied regarding birds performance were feed intake, weight gain and FCR. All the growth performance parameters were recorded on weekly basis while the Ileal digestibility and gut health parameters were recorded at the 35th day of the trial. Data was analysed through completely randomized design (CRD) under factorial arrangement using ANOVA technique (Steel et al. 1997). Means were separated through Duncan’s Multiple Range test (Duncon’s, 1955) with the help of SAS 9.1.
The results of feed intake, weight gain and feed intake: gain on weekly basis showed inconsistent results, however the overall performance of the birds fed low protein diets supplemented with protease showed significant results. The gut health parameters showed the significant increase in villus height. The apparent Ileal digestibility of protein was significantly increased in the MP supplemented with protease enzyme.
36
CONCLUSION
Based upon the findings of this study it can be concluded that;
Supplementation of protease improves growth performance of the broilers however the results on weekly basis were inconsistent but overall growth performance data shows significant results.
Protease supplementation enhances the CP digestibility in 19 % diets compared to 21 % and 17 % CP diets.
The supplementation of protease enzyme improves the villus height and crypt depth: villus height in broilers.
SUGGESTIONS AND RECOMMENDATIONS
Future researchers can further evaluate the subsequent effects of the protease on growth performance and immune response in broilers.
In future the research can be conducted to determine the effect of protease on energy digestibility and immune response in broilers. Availability: Items available for loan: UVAS Library [Call number: 2601-T] (1).
355.
Effect Of Post Pelleting Liquid Phytase Supplementation On Growth Performance And Phosphorous Utilization In Broilers
by Muhammad Balal Munawar (2014-VA-516) | Prof. Dr. Saima | Dr. Shafqat Nawaz Qaisrani | Prof. Dr. Athar Mahmud.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Poultry sector is one of the most developed and vibrant segment of agriculture sector in
Pakistan and is claimed to be among the largest industries of Pakistan with over more than 200
billion rupees investment and offering employment directly or indirectly to 1.5 million people. In
vegetable sources used in poultry rations, P is mostly present in phytate form. Phytate P is not
digested by poultry due to lack of endogenous phytase enzyme. So exogenous phytase are now
added in feed to release P from phytate. Phytase is mainly derived from fungi (Aspergilus niger)
and bacteria (Escherichi coli). Phytase starts work either at 3rd C (3-Phytase) or at 6thC (6-Phytase)
of phytate Phytase mostly used in pre pelleting powder form but it has some activity issues during
high pelleting temperature. Steam application is the most important factor that reduces enzyme
activity due high steam temperature and addition of moisture ultimately lead to enzyme inactivity.
Post pelleting liquid enzyme can be one solution to combat enzyme activity issue.
In total, 504 1-d-old chicks were procured from a commercial hatchery and randomly
assigned to six dietary treatments with Hubbard straight run n=84 chicks per treatment. Each
treatment was further divided into 3 replicates with 28 birds/replicate. One treatment was served
as positive control, while second was served as negative control with (0.30 and 0.26 %) P in starter
and grower respectively. Other four diets had two levels (500 and 750 FTU/kg) of each powder
and liquid enzyme. Liquid enzyme supplementation was done post pelleting. Pelleting temperature
was kept at 85o C.
Feed intake, body weight gain and FCR was recorded at the end of each week. At 35th day of
experiment, 3 birds per replicate were selected randomly, weighed and slaughtered. At the time of
slaughtering, blood samples were collected in heparinized tubes for the determination of plasma
SUMMARY
40
P.Ileal digesta from the slaughtered birds was collected for the determination of P digestibility.
The visceral organs and left tibia from the slaughtered birds were collected, organ weight was
recorded and tibias were ashed for determination of Calcium and P.
Data collected was analyzed using analysis of variance (ANOVA) technique under
Completely Randomized Design (CRD) and means were compared through DMR test by
using SAS 9.1 version (SAS 2001).
The conducted study showed 49.45%, 64.64%, and 8.64 % higher results in feed
intake, body weight gain and FCR when best group H compared with group A (NC). Liver
and heart weight increase in group A, however gizzard weight showed non significant
(p>0.05) results among all groups. Tibia ash, Phosphorous, calcium and plasma P showed
significant results (p<0.05) when all phytase supplemented diets compared with NC. Availability: Items available for loan: UVAS Library [Call number: 2631-T] (1).
356.
Nutrition and Feeding of Organic Cattle
by Blair, Robert.
Edition: 1st ed.Material type: ; Literary form:
not fiction
Publisher: UK: CABI; 2011Availability: Items available for loan: Pattoki Library [Call number: 636.2085 Blair 31782 1st 2011 A.Nutrition] (1).
357.
Nutrient Requirements of Poultry / 9th ed.
by National Research Council (U.S.).
Edition: 9th reprint edition.Material type: ; Literary form:
not fiction
Publisher: Washington: National Academy Press; 2000Availability: Items available for loan: Pattoki Library [Call number: 636.50852 NRC 16449 9th 2000 Animal.Nutrition] (1). Checked out (1).
358.
Hunt's Animal Nutrition and Feeding
by Robbins, T.
Edition: 1st ed. Material type: ; Literary form:
not fiction
Publisher: India: Medtech; 2016Availability: Items available for loan: Pattoki Library [Call number: 636.085 Robbins 32588 1st 2016 A.Nutrition] (5).
359.
Nutrition Experiments in Pigs and Poultry : A Practical Guide
by Bedford, Michael R | O'Neill, Helen Masey [Contributor; Editor] | Choct, Mingan [Contributor; Editor].
Edition: 1st ed. Material type: ; Literary form:
not fiction
Publisher: UK: Cabi; 2016Availability: Items available for loan: Pattoki Library [Call number: 636.40892 Bedford 31947 1st 2016 A.Nutrition] (1).
360.
Effect Of Different Selenium Sources On Nutritive Composition Of Breast And Thigh Meat In Varieties Of Aseel Cockerels
by Muhammad Tayyab (2014-VA-809) | Prof. Dr. Anjum Khalique | Dr. Yasir Allah Ditta | Dr. Jibran Hussain.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Poultry industry is second largest enterprise of Pakistan owing to be a major source of food in terms of meat and egg and shares an enough contribution towards national GDP, livestock growth and mass employment. Indigenous poultry breeds also have been playing important role in fulfilling the protein requirements of people. Aseel is one of the famous breed of Pakistan and distributed throughout the country in its different varieties. Selenium enriched food items are gaining public interest due to health benefits and longer shelf life. Production of such enriched food items is requiring latest knowledge about supplementation of micro minerals in the diet of birds. For this purpose, a study was conducted with basic objective to determine the effect of different selenium sources on meat quality and composition in four varieties and Aseel chicken. Two hundred day old Aseel chickens cockerels were procured from Avian Research and Training Center. After three weeks of brooding and adaptation period, 120 Aseel cockerels were then randomly selected and separated into four groups (40 birds/group/variety), every group was further subdivided into three treatment sub groups (A, B, and C) with 10 birds in each treatment and were assigned three dietary treatments. Aseel cockerels were housed individually in cages system. Group A and B were experimental groups while group C was control. The Aseel chickens were slaughtered at 20 weeks of age and meat of breast and thighs were used for proximate analysis (CP, DM, Ash, Fat) and minerals (micro and macro) determination. Values obtained were used to analyze through two-way ANOVA technique using SAS 9.1 software. Means were compared with DMR test. Result of this study showed that DM% of breast was higher (P≤0.05) in Mushki × SS and of thigh meat higher (P≤0.05) in Peshawari × SS. Similarly, CP of breast meat was higher (P≤0.05) in Lakha × SS and of thigh meat Lakha SY
SUMMARY
54
group and fat of breast was higher in Lakha × SY group and of thigh meat was higher (P≤0.05) in Mianwali × SS group. Varieties differed in macro-minerals i.e., sodium, potassium, phosphorus and calcium shown by Lakha variety in breast meat while magnesium was higher in breast meat of Mianwali. In thigh meat, sodium, potassium, calcium contents were prominent (P≤0.05) in Lakha while magnesium was higher in Mushki variety. However, phosphorus was non-significant in thigh meat. In micro-minerals, selenium contents in breast and thigh meat of Aseel chicken were higher (P≤0.05) in Lakha variety while for copper contents, breast of Lakha and thigh of Mushki variety had higher values (P≤0.05). Zn, ferrous and Mn contents were non-significant in breast meat while in thigh meat, Mn among was higher in Lakha variety. In breast meat, pH, Lightness, Chroma, Hue, Redness and Yellowness were higher in Peshawari, Mushki, organic × lakha, Organic× Mushki, Inorganic × lakha and organic × Mushki groups respectively while, in thigh meat, Mianwali, Mushki ×inorganic selenium, Lakha × inorganic selenium, Peshawari × Organic selenium, Peshawari × inorganic selenium and Lakha × organic selenium had highest values. From above results, we concluded that inorganic selenium is more beneficial in indigenous Aseel chicken as it improve the DM and CP contents while organic selenium is more helpful due to more fat contents in meat of chicken breast and thigh parts. While, Aseel Lakha chicken is strongly recommended for micro and macro mineral enriched meat production.
CONCLUSION
Based upon the finding of the present study it can be concluded that:
Organic selenium helps in improving breast fat and thigh protein in Aseel rooster.
Selenium don’t have any detrimental effect on minerals contents, pH and color of meat of Aseel rooster.
55
Organic selenium better retained in breast and thigh meat than inorganic selenium.
Meat of Lakha Aseel is more nutritive than others varieties fed organic selenium.
SUGGESTION AND RECOMMENDATIONS
Based upon the result of the present study it is recommended that:
This study would be helpful for poultry meat processing industry because Se not only improve meat quality but also shelf life.
Organic selenium should be added in the diet of chicken to improve the nutritive value of meat.
Lakha and Mushki Aseel should be reared for beneficial effect of meat nutrient on human health.
Selenium yeast enrichment in the Aseel feeds can be very helpful increase in body weight which ultimately increase the profitability. Availability: Items available for loan: UVAS Library [Call number: 2729-T] (1).
361.
Animal Breeding / 4th ed.
by Laurence M Winters.
Edition: 4th ed.Material type: ; Literary form:
not fiction
Publisher: New York: Chapman Hall Ltd; 1958Availability: Items available for loan: UVAS Library [Call number: 636.0821 Laurence 5541 4th 1958 Animal.nutrition] (1).
362.
Feeds And Feeding
by Arthur E. Cullison | Arthur E. Cullison.
Edition: 2ndMaterial type: ; Literary form:
not fiction
Publisher: America; Raston |Publishing ; 1979Availability: Items available for loan: Pattoki Library [Call number: 636.084 Cullison 11019 2nd 1979 Anima nurtrition] (1).
363.
Animal Nutrition
Edition: 7th/ed.Material type: ; Literary form:
not fiction
Publisher: New Delhi: The McGraw-HI;; 1986pAvailability: Items available for loan: Pattoki Library [Call number: 636.085 Maynard, 12969 1986 7th Animal Nutrition] (1).
364.
Animal Nutrition
Edition: 7th/ed.Material type: ; Literary form:
not fiction
Publisher: New Delhi: The McGraw-HI;; 1986pAvailability: No items available
365.
Efficacy Of Different Microbial Phytases On Growth Performance Carcass Characteristics And Mineral Retention In Broilers
by Muhammad Faisal Rafique (2014-VA-918) | Dr. Talat Naseer Pasha | Dr. Saima | Dr. Athar Mahmud.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Cd Crupt Availability: Items available for loan: UVAS Library [Call number: 2762-T] (1).
366.
Effect Of Microencapsulated Butyric Acid Supplementation On Growth Performance, Ileal Digestibility Of Protein, Gut Health And Immunity In Broilers
by Muhammad Imran (2009-VA-417) | Dr. Saeed Ahmed | Dr. Yasir Allah Ditta | Dr. shahid Mehmood.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: In Pakistan poultry industry is well established and organized sector of agriculture, with more than 200 billion rupees investment among the largest industries and claimed largest industry in Pakistan . The need of the recent poultry industry are high levels of production and efficient feed conversion ,which to a certain extent could be attained by the consumption of specific feed additives like (OA). One of the effective feed additive in poultry ration is Butyric acid escepecialy encapsulated butyric acid because coated have extended release at target site .Microencapsulated butyric is tool to improve Growth performance and gut health in broilers . TheBA is a readily available energy source for intestinal epithelial cells and stimulates their differentiation and multiplication consequently it improves broilers performance.The biological trial was conducted at Research and development Farm Sharif Feed Mills (Pvt) Ltd, Okara for the duration of 35 days. In total, 336 1-day-old broiler chicks were procured from a commercial hatchery and randomly assigned to 12 floor pens on a concrete floor with rice husk as a bedding material. Four dietary treatments containing microencapsulated butyric acid (0.00, 0.25, 0.35 & 0.45g/kg × three replicate pens each having 28 chicks). There was one feeding trough and six water nipples in each pen. On 7th day of the experiment, feeding troughs were replaced with a round bottom feeder. All standard management practices were followed through the trial. Birds were vaccinated according to prescribed schedule. Weekly body weight and feed intake was recorded to calculate the weekly body weight gain and feed conversion ratio. At the end of experiment, two birds were randomly picked from each pen and slaughtered for the collection of serum in evacuated tube, duodenal samples and ileal digesta. These duodenal samples preserved in 10% formalin for tissue processing and Ileal digesta samples stored at -20C for AIA and CP.The collected data were analyzed through completely randomizes design (CRD) under one way ANOVA technique. Means were separated through Duncan’s Multiple Range test with the help of SAS 9.1.3.in conclusion we can say that Addition of microencapsulated butyric acid in broilers diet improved body weight gain, feed conversion ratio, gut health and apparent ileal digestibility of protein but no significant effect was observed on antibody titer against Newcastle disease.
Suggestion and recommendation
Further research is needed to evaluate the protected butyric acid on enzyme secretion and starch utilization, along with other nutrient digestibility in broilers
Availability: Items available for loan: UVAS Library [Call number: 2763-T] (1).
367.
Effects Of Varying Dietary Crude Protein Supplies On Performance Of Lactating Nili-Ravi Buffalo Fed High Forage Based Diet
by Muhammad Uzair Akhtar (2008-VA-175) | Dr. Anjum Khalique | dr. Muhammad Qamar Shahid.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Buffaloes contributed 61.1 percent to the total milk of Pakistan, which is world’s 4th largest milk
producing country. Protein requirements of buffalo appear to differ from those of cattle, yet
information on these nutritional requirements is limited. But establishing the protein and energy
requirements for animals is key point to lead the nutritionists for the optimum supply of these
nutrients. Thus, understanding the protein and N efficiency and an estimation of optimum protein
supply to buffalo is the need of time in terms of economics and animal production.
This study was conducted at “Dairy Animals Training and Research Center, University of
Veterinary and Animal Sciences, Ravi Campus, Pattoki” to check the performance of buffaloes in
response to increasing levels of dietary crude protein (CP) supplies (9.3, 10.0 and 11.5% of DM).
Design of the experiment was 3×3 Latin Square with two animals in each treatment, and total
duration of the study was 119 days. Different parameters were determined by using different but
recommended laboratory techniques. Milk parameters include milk yield, milk protein, fat, lactose
and density. Blood parameters include BUN, TG, NEFA and glucose. Fecal and urine sampling
and tests were performed using standard procedures to estimate the N efficiency and N balance.
Data was analyzed using GLM procedures of SAS.
DMI was similar among all the treatments with an average value of 14.36 kg/d. Milk production
increased from 7.75 to 8.04 kg/d in response to increase in dietary CP. Milk protein contents
increased from 3.86 to 3.92%, milk fat contents from 6.12 to 6.44 % and 4% fat corrected milk
from 10.05 to 10.92 kg/d, but milk lactose and milk density showed no response to increase in
dietary CP from 9.3 to 11.5% of DM. However, milk lactose yield increased, but body weight of
the animals decreased from 586 to 565 kg. Milk N efficiency also decreased from 21.9 to 18.7%,
SUMMARY
44
but an increase in N intake observed with higher CP levels. Feed efficiency also increased from
0.54 to 0.56.
Blood metabolites showed a positive response to increase in dietary CP. Glucose, BUN and NEFA
increased with increase in protein supplies. While, TG contents were not affected by the
treatments. Milk N excreted as gram per day increased but decreased as percent of N intake,
resulting in reduced N efficiency. Urinary and fecal N excretion as gram per day also showed a
slight linear increase. Retention of N remained unaffected by the treatments but the values are
higher than that of cow, still a point reported to be attached with genetics of buffalo to retain more
N in the form of BUN as its reserves.
Increasing CP supplies improved both of the milk quantity and quality, and resulted in increased
BUN, glucose, NEFA and N intake, but decreased N efficiency. In this sense, when buffalo is
compared with cow, cow utilizes its dietary N in better way than buffalo. But when we estimate
lactation performance of both, at different low and high levels of protein supplies, buffalo performs
better at low level of dietary CP as compared to high protein diet. Current experiment served as
opportunity to recommend the minimum level of dietary CP for buffaloes without effecting the
animal production to minimize the cost and environment wastage.
CONCLUSIONS
In conclusion, increase in dietary protein supply results in improved quantity and quality of milk,
but meanwhile this increased supply causes a decrease in nitrogen use efficiency, because protein
supply above the required level starts to be excreted in urine and feces. And 11.5% CP of ration
DM is sufficient for Nili-Ravi buffalo at this production status. Availability: Items available for loan: UVAS Library [Call number: 2764-T] (1).
368.
Horse Feeding And Nutrition
by Cunha, Tony J.
Edition: 1st ed.Material type: ; Literary form:
not fiction
Publisher: NewYork: Academic Press; 1980Availability: Items available for loan: UVAS Library [Call number: 636.1085 Cunha 11171 1st 1980 A.Nutrition] (1).
369.
Effects Of Digestible Lysine Levels On Production Efficiency, Protein Digestibility, Gut Health And Carcass Characteristics In Broilers Fed Different Protein Sources
by Muhammad Jhazaib Arslan (2014-VA-956) | Dr. Shafqat Nawaz Qaisrani | Prof. Dr. Saima | Prof. Dr. Athar Mahmud.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: In Pakistan, poultry is a vibrant part of agriculture. Nutritional and genetic improvements resulted in accelerated PEF in broilers. This improved broiler production efficiency requires greater amount of digestible nutrients like digestible amino acids. Feeding cost accounts for 70% of total cost and protein is the most expensive component of broilers ration. Future poultry rations will be formulated by using poorly digestible and cheaper protein sources such as SFM and CM due to price instability of highly digestible sources like SBM. The undigested protein in hind gut subjected to microbial fermentation, results in formation of nitrogenous products that are harmful to birds. The possible solution to this problem is supplementation of synthetic amino acids in low nutrient density (CP) diets formulated by using cheaper and poorly digestible protein sources. Therefore a study was designed with the objectives to determine the impact of dLys on production efficiency, TTP digestibility, gut health and carcass characteristics in broilers fed poorly digestible protein sources (CM and SFM). An experiment with 624 Ross 308 1-d-old broilers was conducted to test the hypothesis that supplementation of dLys may improve production efficiency, TTP digestibility, gut health and carcass characteristics of the broilers fed poorly digestible protein sources (CM and SFM). The interaction effects of the protein source (CM vs. SFM), CP levels (high vs. low) and dLys (high, medium and low) were tested in a factorial arrangement of 12 (2 × 2 × 3) dietary treatments and 1 control treatment. The supplementation of dLys positively influenced production efficiency (P <0.001). The birds fed diets containing 19% CP showed 8.53% greater TTP digestibility compared with those fed diets with 17% CP, however, VH was influenced by protein source (P<0.001) and CP level (P = 0.030), however, not influenced by dLys levels while CD was influenced by dLys levels (P = 0.023). The birds fed 1.16% dLys supplemented diet had a 9.6 and 7.5% greater dressed weight with giblets compared with those birds fed diets with 1.10 and 1.21% level of dLys. In conclusion, supplementation of dLys improves the broiler production efficiency fed poorly digestible protein sources (CM and SFM). The negative effects of poorly digestible protein sources thus may be partially ameliorated by supplementation of dLys.
CONCLUSIONS
Supplementation of dLys above the recommended levels (1.16%) improves the production efficiency, TTP digestibility of protein, gut health and carcass characteristics in broilers fed poorly digestible protein sources (CM and SFM). In conclusion, the efficiency of poorly digestible protein sources may be improved by supplementation of dLys at level of 1.16%.
Availability: Items available for loan: UVAS Library [Call number: 2765-T] (1).
370.
Comparative Evaluation Of Effect Of Selenium Sources On Growth, Production And Reproduction In Different Varieties Of Indigenous Aseel Chicken
by Muhammad Waseem Zia (2012-VA-652) | Prof. Dr. Anjum khalique | Prof. Dr, Saima | Dr. Jibran Hussain.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: Universally, domestic chickens are considered very important due to their better adaptability to the local climatic conditions. Among native poultry breeds of Pakistan, Aseel is a recognized native breed used mostly for backyard poultry production and is famous for its appreciable degree of resistance to various diseases as compared with commercial exotic poultry breeds. In many communities, native chickens are considered very important in breaking the vicious poverty cycle. This breed contains genes and alleles appropriate to their adaptation to the specific environment. On the basis of its characteristic of producing good quality meat, Aseel chicken can be used and further improved as a meat type bird. However, the main limitation in its propagation is its slower growth rate, poorer egg production, smaller egg size, lower fertility and hatchability.Selenium (Se) is an essential micro-mineral for poultry which exists in organic and inorganic forms. Organic Se is reported to be more bio-available which improves the growth performance, productive and reproductive potential of poultry resulting in better egg and meat production as well as the quality. Despite having considerable capabilities, inadequate research work has been so far conducted on Aseel that may be the main bottleneck towards increasing its overall production. In this study, there was a focus on the improvement of growth, productive and reproductive potential of Aseel; also for the development of functional poultry products (eggs and meat, enriched with Se). The present study was planned with the main objectives to investigate the influence of organic (Se-enriched yeast: SY) and inorganic (sodium selenite: SS) Se dietary supplements on growth performance, nutrient utilization, blood biochemical profile, slaughter characteristics, tissue Se concentration, rearing expenditures andmortality as well as the production traits including egg production, egg quality/geometry, hatching traits and egg Se concentration in four recognized varieties namely Lakha, Mushki, Peshaweri and Mianwaliof indigenous Aseel chicken. The study comprised two main experiments conducted at the Indigenous Chicken Genetic Resource Centre (ICGRC), Department of Poultry Production, University of Veterinary and Animal Sciences (UVAS), Ravi campus, Block-C, Pattoki with the objectives to explore theimpact of dietary Se addition, influence of varieties and their interaction on overall performance of Aseel chickens. For this purpose, in the first experiment, 240 three-week-aged birds (120 males and 120 females) were distributed in a randomized complete block design into four groups, 60/variety (30 males + 30 females). These birds of either sex were further subdivided into three treatment groups A, B and C according to the nature of diet (SY added, SS added and without Se added) with 10 birds/treatment and each treatment was replicated 10 times with one bird in each replicate. Data thus collected were subjected to analysis of variance techniques in a randomized complete block design and means were compared using Duncan’s Multiple Range test. The studies regarding overall response to Se supplementation for growth performance depicted substantial influence. Dietary addition with SY increased the nutrient retention, feed conversion, body weight and decreased the mortality and rearing expenditures, especially in the males of Lakha variety. All body measurementswere improved in the birds received SY supplemented diet, the SY-fed males of Lakha exhibited better response compared to rest of the treatments.SY addition significantly increased the values of glucose, triglyceride, globulin, glutathione peroxidase (GPX) in blood serum, while significantly decreased levels of urea, creatinine, uric acid, alanine aminotransferase (ALT), aspartate aminotransferase (AST), total cholesterol, and thyroxine (T4) in the serum were observed in SY-fed birds, especially in the birds of Lakha and Peshaweri varieties. However, total protein and albumin values in the serumwere foundunaffected by Se supplementation. The significant variations in the slaughtering traits were observed; SY inclusion presented improved live final body weight, dressing weight, dressing percent, eviscerated weight, eviscerated percent and giblet weight. Selenium uptake by the chest and thigh muscles was also enhanced in the male birds of Lakha and Mushki varieties exposed to SY added diet. The rearing cost of the birds in SY-fedgroup was noticed lower than the cost recorded for those got SS supplemented or control diet. The males of Lakha in this regard presented the least rearing cost on receiving SY supplemented diet.
The second experiment was also conducted with the objectives to compare the influence of organic and inorganic Se inclusion, varieties and their interaction on productive performance, egg geometry/quality traits, reproductive performance and the egg Se contents in Aseel. For this purpose, 96 twenty-one-weeks old birds (84 females and 12 Males) from Lakha, Mushki, Peshaweri and Mianwali varieties of Aseel were selected and distributed under randomized complete block design into three groups, 24/variety (21 females + 3 males); further subdivided into three treatment groups, 7 females and 1 male in each treatment according to Se treatments (SY, SS and control) and each treatment was replicated 7 times with one bird in each replicate. Each bird was regarded as an experimental unit. The data thus collected were subjected to analysis of variance in a randomized complete block design and means were compared using Duncan’s Multiple Range test. The results showed that the females of Mianwali variety fed with SY supplemented ration exhibited higher feed intake, enhanced body weight and gained sexual maturity earlier; furthermore, Mianwali variety exhibited better egg production, showed higher egg weight and egg mass. FCR/dozen eggs, FCR/kg egg mass were as observed to be higher in the females of Peshaweri variety. Egg Se concentration was higher in the SY supplemented group as compared with SS or control groups.Dietary Se supplementation significantly influenced the shape index, yolk index, Haugh Units and eggshell thickness. However, non-significant variations were observed in Haugh Units among the females of all varieties. Interaction results presented the significant differences in egg breadth, egg length, egg volume, egg weight, egg shape index, egg shell thickness, yolk index and Haugh Units in the females of all varieties; with significantly higher values in the females of Mianwali variety exposed to SY added ration.The reproductive performance pertaining to hatching traits depicted that the Se supplements, varieties and their interaction influenced the hatching traitssignificantly. The SY-fed females of Peshaweri presented the decreased dead germ percent, dead in shell percent and clear egg percent compared with the females got SS supplemented ration or unsupplemented control ration. Improved hatchability percent and the hatch of fertile percent were noticed in the females of SY supplemented group than those of SS or control groups and the greatest increase was recorded in the females of Peshaweri variety. Similarly, enhanced body weights by the newly hatched chicks of Peshaweri and Mianwali varieties were observed fed with SY supplemented ration and also in the interaction, significant results were recorded.
9.1 CONCLUSIONS
Based upon the findings of the present study, it can be concluded that all four varieties of indigenous Aseel responded positively to the dietary addition with SY supplements of Se regarding growth performance of Aseel, especially in the male birds of Lakha variety. SY supplementation also showed the better results regarding productive and reproductive performance, especiallyin the females of Mianwali and Peshaweri varieties with more promising results. Moreover, SY addition displayed the substantial results in shape of production of functional foods such as Se-enriched eggs and meat.
9.2 SUGGESTIONS AND RECOMMENDATIONS
In future, indigenous Aseel chicken raising programs should be given due emphasis while proceeding for poultry farming by using native breeds of poultry.
9.2.1 Recommendations for the farmers
Keeping in mind the results of this study, here are some recommendations for the poultry farmers.
1. Due to the large body size of Aseel and a good ability to show resistance against a variety of infectious diseases, many poultry farmers want to rear and use Aseel as a parent flock for further breeding but are hesitant to go for that because of its slow growth. Slow growth is due to its low feed nutrient utilization and consequently slow gain in body weight. Lower production performance is mainly due to its late sexual maturity, long pauses and short clutch size.In the current situation,the strategic inclusion of Se in the diets of Aseel, being the effective technology, can help the farmers overcome the problems of slow growth and low productive performance in Aseel by synchronizing and consequently bringing the Aseel birds into better growth and production. It can help the farmers to get the production of the Aseel birds, according to their need and situations.
2. In the current study, SY has been substantiated to improve the reproductive performance of Aseel, hence can be used further to elucidate the very old concern of lower fertility and hatchability in Aseel parent flock consequently improving progeny size.
3. Keeping in view the above, the Aseel can be used as a feasible option for the poultry business by enhancing its sluggish growth rate and lower production potential through Se supplementation as well as to lower fertility and hatchability through the supplementation of organic Se supplement (SY) in the diet. Ultimately, it will be remained supportive towards the revival of open- sided poultry farms that have become closed after the trend to establish the environmentally controlled sheds and are lying vacant from a long time.
9.2.2 Suggestions for the researchers
1. In the current study, the fixed levels ofSe supplements were used for supplementation, whereas future researchers can work on different levels of it.
2. Further trials may be planned to check the subsequent effects of different sources of Se supplements on the biochemical profile of meat and eggs; also the immune response in the chicks from hens reared on Se supplemented diets.
3. The evaluation of semen quality in Aseel males and the impact of artificial insemination in the hens by the semen received from Se treated males of Aseel can also be studied.
Availability: Items available for loan: UVAS Library [Call number: 2780-T] (1).
371.
Urea and non-protein nitrogen in ruminant nutrition.
by Stangel, Harvey J; Johnson, Ronald R | Stangel | Stangel, Harvey J; Johnson, Ronald R.
Edition: 2nd/ed.Material type: ; Format:
print
; Literary form:
not fiction
Availability: Items available for loan: Pattoki Library [Call number: 636.085 Harvey 11552 2nd Animal Nutrition] (1).
372.
The Behaviour Of Domestic Animals
by E.S.E Hafeez.
Edition: 1st/ed.Material type: ; Literary form:
not fiction
Publisher: London: Balliere: 1969Availability: Items available for loan: Pattoki Library [Call number: 636.082 E.S.E 10052 1st 1969 Anila Nurtrition] (1).
373.
Influence Of Digestible Lysine Levels On Growth Performance, Gut Development And Ileal Digestibility Of Two Vegetable Protein Sources Fed To Broiler Birds
by Anjum Faiz (2014-VA-955) | Dr. Shafqat Nawaz Qaisrani | Prof. Dr. Saima | Prof. Dr. Athar Mahmud.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: The poultry sector is playing a substantial role in agriculture sector of Pakistan. The extensive
research in poultry has brought vital changes that accelerated growth and improved FCR. Poultry
diets are majorly formulated cost effective for the sustainable poultry industry. For this reason,
the main focus is on protein sources which, if maintained to an adequate level with the
requirement results in cost effective feeding. In the coming days, main focus of the nutritionists
would be on replacement of expensive sources of protein during feed formulation. For this
reason, their first choice may be the selection of poorly digestible protein sources such as CM
and SFM. These sources are cheaper as compare to the SBM which is imported hence increasing
the feed cost. The poorly digestible protein sources, furthermore, in hind gut may be subjected to
microbial fermentation, results in formation of nitrogenous products that are not only harmful to
birds, but also excreted into litter bedding and generate ammonia, nitrous acid and nitrous oxide,
the latter one is a powerful greenhouse gas with an effect about 300 times compared with carbon
dioxide. The potential solution to this problem is supplementation of synthetic amino acids in
low nutrient density (CP) diets formulated by using cheaper and poorly digestible protein
sources. For this reason, a study was designed with the objectives to determine the impact of
dLys, on growth performance, ileal digestibility of protein and gut development in broilers fed
poorly digestible protein sources (CM and SFM). An experiment with 624 mixed sex 1-d-old
broilers (Ross 308) was conducted to test the hypothesis that supplementation of dLys may
improves growth performance of the broilers fed poorly digestible protein sources (CM and
SFM). The interaction effects of the protein source (CM vs. SFM), CP levels (high vs. low) and
dLys (recommended vs. supplemented) were tested in a factorial arrangement of 12 (2 × 2 × 3)
dietary treatments and SBM as a control diet was used. The results indicate the birds fed diets
DISCUSSION
42
containing CM and SFM supplemented with dLys has significant effect on growth performance
in broilers. The results of the study showed that the FI, BWG and FCR for CM and SFM based
diets was different during starter and grower phases. The FI and BWG FCR was influenced by
CM and SFM based diets. The FI was not influenced by dLys levels during starter phase. The
BWG and FCR was improved with supplementation of dLys levels. During starter phase FI and
BWG was not influenced by CP levels, however, FCR was improved. The results of the grower
phase indicate that FI was significantly influenced by protein sources, CP levels, as well as, by
dLys levels. The BWG, similarly, was significantly influenced by protein sources, CP levels, and
dLys levels. The FCR during the grower phase was improved by increasing CP level. The FCR
was also improved when fed CM based diets. Supplementation of dLys significantly influenced
FI and BGW during grower phase. The FCR was also improved with the supplementation of
dLys levels. The results indicate that the birds fed dLys supplemented diets had significant effect
on ileal digestibility of protein. The results of the study showed that ileal digestibility for protein
was not influenced by CP levels. The present study resulted that some of the organs are
significantly influenced by dLys levels and CP levels, whereas, other are in influenced by protein
sources. Amino acid supplementation showed significant difference on ileal digestibility of
protein and gut development in broilers. The negative effect of poorly digestible lysine can be
compensated with the supplementation of synthetic amino acids including lysine.
CONCLUSIONS
Supplementation of dLys above the recommended levels improves the growth performance, ileal
digestibility of protein and gut development in broilers fed poorly digestible protein sources (CM
and SFM). In conclusion, the negative effects of poorly digestible protein sources in broilers may
be ameliorated by supplementation of dLys. Availability: Items available for loan: UVAS Library [Call number: 2776-T] (1).
374.
Effect Of Different Levels Of L-Valine On Production Performance, Serum Biochemistry And Illeal Digestibility Of Protein In Laying Hens (Lsl - Lite)
by Usman Liaqat (2015-VA-1092) | Dr. Yasir Allah Ditta | Dr. Shafqat Nawaz Qaisrani | Dr. ShahzadAli.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: Valine is a limiting branched amino acid in corn soya layer diet.It has both properties of glycogenic and ketogenic. It is also important in protein synthesis and precursor for many amino acids. A very little research has been conducted on levels of valine in layer strains globally, particularly research data is limited in Pakistan. So far, no trial has been conducted to find out the effect of different levels of valine for LSL lite in Pakistan.
A total of 490 laying birds of LSL (LITE) strain of 33 weeks of age were selected and categorized into seven dietary treatments with different levels of Valine i.e. 0.70, 0.75, 0.78, 0.81, 0.84, 0.87 and 0.90 % of feed. All experimental diets were fed in mash form for 56 days of experimental period. Celite was added at rate of 2 % of feed for crude protein digestibility determination. At the end of experiment, two birds per replicate were randomly selected and slaughtered for the collection of blood and illeal digesta. Egg weight and egg production were calculated on daily basis. Three egg were selected from each replicate to measure egg quality parameters at end of each week. The egg quality parameters were calculated on weekly basis and body weight was measured at beginning, mid and end of the experimental period.At day 56, two birds from each replicate under different treatments were randomly selected and slaughtered to collect sample for ileal protein digestibility and blood for serum biochemistry. Ileal samples were
stored at -20C. Serum samples were extracted and stored at -20C for measuring serum chemistry. Collected data were subjected to ONE WAY ANOVA under Complete Randomized Design. Duncan’s multiple range tests (DMR) were used to compare means. Statements of statistical significance are based on P < 0.05. Weekly collected data of egg quality parameters were analyzed by repeated measures SAS (version 9.1; SAS Inst. INc., Cary, NC).
The current study shows significant influence of valine on production performance of laying hens. With increase in valine levels in the diet of laying hens, the egg production, FCR and feed intake significantly improved.Valine has significantly enhanced egg quality parameters i.e. egg weight, Haugh unit, egg yolk color, specific gravity, proportional yolk weight, proportional shell weight, eggyolk index and egg yolk to albumen ratio. Significant effects on total serum protein, serum albumin, immunity and ileal digestibility of proteins have been observedin laying hens. Following results shows that valine is limiting amino acid afterthreonine in diets devoid of animal protein in corn soya bean dietand must be given in diet for maximum production.
Conclusion
It can be concluded that valine is a limiting branched chain amino acid in corn soya layer diet. Supplementation of valine significantly increase egg production, improved FCR / dozen of laying hens. An increase in valine levels also significantly decreases the feed intake in laying hens which may be due to the antagonistic effects with BCCAA levels. Valine improved egg quality parameters (proportional yolk weight, proportional shell weight, egg weight, yolk color, specific gravity, yolk index and yolk to albumen ratio, Haugh unit) of laying hens. Influence of Valine has been observedon serum biochemistry (serum albumin, serum total protein and ND titer) of laying hens.
Recommendations for the farmers
Based on the current findings, 0.87 % valine is recommended level for maximum egg production at the age of 33-40 weeks of age with dig Val : dig Lys ratio of 1.04 : 1.
Suggestions for the future researchers:
1. Same experiment can be done in broilers to assess growth performance, serum biochemistry and ileal digestibility of protein.
2. Future researchers can also apply this research in different layer strains.
Availability: Items available for loan: UVAS Library [Call number: 2857-T] (1).
375.
Effect Of Feed Form And Particle Size Of Corn On Growth Performance, Protein Digestibility And Gut Health Of Broiler
by Shahzada Muhammad Zeshan Haider (2014-VA-919) | Prof. Dr. Talat Naseer Pasha | Prof. Dr. Saima | Prof. Dr. Athar Mahmud.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: The presented study was conducted at control house of Department of Poultry Production, C-block, UVAS Ravi Campus. In total 480, day-old Hubbard Classic broiler chicks were procured from local hatchery, weighed at 1st day and randomly distributed to eight treatment groups in such a way that there were six replicates in each group and each replicate had 10 chicks. Eight isocaloric and isonitrogenous experimental diets were formulated. Half birds were offered crumble form of feed in whole life while half birds were offered crumble form in starter phase and pellet form in finisher phase. Four levels (<1, 1-1.5, 1.5-2 and 2-2.5mm) of corn particle size were used in each form of feed.Body weight and feed consumptionwere recorded weekly in order to calculate feed conversion ratio.Data collected were analyzed by ANOVA using factorial arrangement under CRD. Experimental means were compared using DMR test.
Birds fed diet having higher particle size (2-2.5mm) of corn had lower feed consumption, greater body weight gain and improved FCR compared to those fed smaller particle size during starter, finisher and overall phase. Further, birds fed pelleted feed had lower feed consumption, greater weight gain and improved FCR than those fed crumble form of feed.Further, different particle size of corn resulted in non-significant effect on villus height and crypt depth. Feed form also had non-significant influence on villus height and crypt depth of duodenum.Birds fed diet having higher particle size (2-2.5mm) of corn had higher apparent ileal digestibilityof protein than those fed smaller particle size. Feed form had non-significant effect on apparent ileal digestibilityof protein.
It can be concluded from the results that birds fed pelleted diet containing coarse particle size had improved growth performance and protein digestibility without adverse effect on gut health of broiler.
Availability: Items available for loan: UVAS Library [Call number: 2879-T] (1).
376.
Studies On Growth Performance And Total Tract Protein Digestibility In As Influenced By Different Dietary Inclusion Levels Of White, Par Boiled And Brown Rice Broken In Broilers
by Omar Javed (2007-VA-154) | Prof. Dr. Talat Naseer Pasha | Prof. Dr. Saima.
Material type: Publisher: 2017Dissertation note: Poultry sector is playing substantial role in agriculture sector of Pakistan. In the present era extensive research in poultry sector has brought vital changes which are accelerating growth and lowering feed conversion ratio. Nutritional improvements in the poultry dietsare improving the growth performance and nutrient digestibility. Poultry diets are majorly formulated cost effective for the sustainability of poultry industry and its consumers. For this reason, the main focus is on using nutritionally rich and locally available raw feed ingredients in broiler diets. Cereal grain are main energy sources used in broiler diets and among cereal grains maize is being used as a major energy source in broiler diets, but its availability, price fluctuations, incidence of mycotoxins are the major concerns and hence, a suitable alternate energy source is required. Rice broken may serve as the substitute, but, a little data is available about the efficacy of different types of rice broken at different dietary inclusion levels regarding growth performance and total tract protein digestibility studies. Therefore a study was designed with the objectives to determine the impact of different types of rice broken (white, parboiled and brown) at different dietary inclusion levels (10%, 30% and 50%) in replacement with maize on broiler growth performance and total tract digestibility of protein. An experiment with 1-d-old 600 Cobb-500 broilers was conducted to test the hypothesis that rice broken from different sources may successfully substitute maize in broiler diets. The effects of the energy sources (white broken rice, parboiled broken rice and brown broken rice) and dietary inclusion levels(10%, 30% and 50%) were tested by using one way ANOVA under Completely Randomized Design and red maize as a control diet was used. The broilers fed different energy sources had significant variation in body weight gain and feed conversion ratio. Better body weight gain and feed conversion ratio was observed in control group followed by white broken rice and lower body weight gain and highest feed conversion ratio was observed in birds fed brown rice broken in diets, similarly total tract digestibility of protein was observed higher in birds fed 50 % maize (control group) and birds fed white rice in diets up to 30% inclusion level and lower total tract digestibility of protein was observed in birds fed brown rice broken in diets. The results of the study showed that red maize is the most beneficial energy source used in broiler diets and white rice broken could be used as good alternative energy source as compared to other types of rice broken i.e. parboiled and brown.
CONCLUSIONS
In conclusion, white rice broken can easily substitute maize up to 30% dietary inclusion level in broiler but other types of rice broken i.e. parboiled and brown give comparatively good results in terms of growth performance and total tract protein digestibility up to 10% dietary inclusion level in broilers.
Availability: Items available for loan: UVAS Library [Call number: 2878-T] (1).
377.
Studies On Growth Performance And Total Tract Protein Digestibility In As Influenced By Different Dietary Inclusion Levels Of White, Par Boiled And Brown Rice Broken In Broilers
by Omar Javed (2007-VA-154) | Prof. Dr. Talat Naseer Pasha | Prof. Dr. Saima | Jibran Hussain.
Material type: Publisher: 2017Dissertation note: Poultry sector is playing substantial role in agriculture sector of Pakistan. In the present era extensive research in poultry sector has brought vital changes which are accelerating growth and lowering feed conversion ratio. Nutritional improvements in the poultry dietsare improving the growth performance and nutrient digestibility. Poultry diets are majorly formulated cost effective for the sustainability of poultry industry and its consumers. For this reason, the main focus is on using nutritionally rich and locally available raw feed ingredients in broiler diets. Cereal grain are main energy sources used in broiler diets and among cereal grains maize is being used as a major energy source in broiler diets, but its availability, price fluctuations, incidence of mycotoxins are the major concerns and hence, a suitable alternate energy source is required. Rice broken may serve as the substitute, but, a little data is available about the efficacy of different types of rice broken at different dietary inclusion levels regarding growth performance and total tract protein digestibility studies. Therefore a study was designed with the objectives to determine the impact of different types of rice broken (white, parboiled and brown) at different dietary inclusion levels (10%, 30% and 50%) in replacement with maize on broiler growth performance and total tract digestibility of protein. An experiment with 1-d-old 600 Cobb-500 broilers was conducted to test the hypothesis that rice broken from different sources may successfully substitute maize in broiler diets. The effects of the energy sources (white broken rice, parboiled broken rice and brown broken rice) and dietary inclusion levels(10%, 30% and 50%) were tested by using one way ANOVA under Completely Randomized Design and red maize as a control diet was used. The broilers fed different energy sources had significant variation in body weight gain and feed conversion ratio. Better body weight gain and feed conversion ratio was observed in control group followed by white broken rice and lower body weight gain and highest feed conversion ratio was observed in birds fed brown rice broken in diets, similarly total tract digestibility of protein was observed higher in birds fed 50 % maize (control group) and birds fed white rice in diets up to 30% inclusion level and lower total tract digestibility of protein was observed in birds fed brown rice broken in diets. The results of the study showed that red maize is the most beneficial energy source used in broiler diets and white rice broken could be used as good alternative energy source as compared to other types of rice broken i.e. parboiled and brown.
CONCLUSIONS
In conclusion, white rice broken can easily substitute maize up to 30% dietary inclusion level in broiler but other types of rice broken i.e. parboiled and brown give comparatively good results in terms of growth performance and total tract protein digestibility up to 10% dietary inclusion level in broilers.
Availability: No items available
378.
Evaluation Of Different Strategies To Improve The Dietary Nitrogen Efficiency In Lactating Dairy Cows In Pakistan
by Muhammad Imran (2005-VA-09) | Prof. Dr. Talat Naseer Pasha | Dr. Muhammad Naveed ul Haque | Dr. Muhammad Qamer Shahid.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: The objectives of this study were to optimize the protein supplies and replacement of SBM with locally available ingredients in the rations of high producing Holstein Friesian cows at mid lactation. On the basis of these objectives, three experiments were conducted. Multiparous cows in mid-lactation received three treatments in a 3×3 Latin square design with a period length of 20 d. Number of animals used were nine in 1st and 3rd experiments and 12 in 2nd experiment (Table 6.1). The trials were conducted at a corporate dairy farm. When we compare the initial and final values of milk yield, milk fat and milk protein contents, there is not a big difference of our diets with that currently being practiced in Pakistan (Table 6.1). This also reveals that the experimental milk production was close to pre-experimental milk production indicating that a successful dietary transition was achieved. Table 6.1: Demonstration of parameters before and during the experiments Exp.
Cows
No.
Initial Parameters During Experiment Parameters
DIM Milk
yield (kg)
Milk
fat (%)
Milk
protein (%)
Milk
yield (kg)
Milk fat
(%)
Milk
protein (%)
1 9 113±25 32±4.1 3.65 3.25 29.7±3.1 3.70 3.27
2 12 153±44 23.3±2.1 3.99 3.34 24.7±1.8 3.98 3.31
3 9 109±19 34±3.7 3.71 3.19 30.7±2.5 3.64 3.21
Exp., experiment; DIM, days in milk In the 1st experiment, three dietary treatments were designed to provide similar energy and increasing supply of MP (g/d)—2371 (low), 2561 (medium), and 2711 (high). Increasing the MP supplies did not modify DMI; however, it increased milk protein, fat, and lactose yield linearly. Similarly, FCM increased (9.3%) linearly due to an increase in both milk yield (5.2%) and milk fat content (7.8%). Milk nitrogen efficiency decreased from 0.26 to 0.20, whereas, the
Summary
102
metabolic efficiency of MP decreased from 0.70 to 0.60 at low to high MP supplies and it average value across the treatments was 0.64 (Table 6.2). In conclusion, increasing the MP supplies resulted in increased milk protein yield; however, a higher BUN and low MNE indicated an efficient utilization of dietary protein in low MP supplies. Milk nitrogen efficiency ranges from 20 to 30% in dairy cows at mid stage of lactation. Milk nitrogen efficiency increases slightly but linearly with the increase of dietary protein up to a certain level of supply of protein. At high protein levels of dietary protein MNE is low and vice versa. In the 2nd experiment, the response of balancing metabolizable Lys to Met ratio (3:1) in low protein diets was investigated. Three experimental diets; 1) LP−: low protein diet (13.6% CP) with imbalanced Lys to Met ratio (3.33), 2) LP+: low protein diet (13.5% CP) with balanced Lys to Met ratio (2.94) through HMBi; and 3) HP−: high protein diet (14.7% CP) without balancing Lys to Met ratio (3.39) in a 3×3 Latin square design were designed. Milk yield of LP- was 0.85 kg/d less as compared with the average milk yield of LP+ and HP-. Dry matter intake decreased by 0.7 kg/d in LP+ compared to HP- treatment whereas milk yield tended to be higher by 0.7 kg/d and protein yield by 23 g/d. Balancing the Lys to Met ratio by supplementing HMBi through feed increased feed, N, and MP conversion efficiencies to milk by 4.4, 1.6, and 13.1% respectively compared to the HP- diet. Similarly, 4% FCM was increased by 4.4% in LP+ diet as compared to HP- diet. Moreover, plasma urea concentration was numerically less in LP+ compared to LP- and HP- treatments whereas no effect was observed on plasma glucose and TG concentrations. In the 3rd experiment, three diets 1) Control: with low protein with SMB as a protein source, 2) SBMD: high protein diet with SBM as a major protein source and 3) CGMD: high
Summary
103
protein diet with CGM as a major protein source. Increasing the protein supplies did not affect DMI, milk fat yield, and milk fat and lactose contents in SBMD and CGMD diets compared to the control diet. Similarly, MP balance and MP/NEL increased by 31.5 and 9.1%, respectively. Increasing the protein supplies tended to increase milk yield. Similarly, milk protein and lactose yield increased by 3.5 and 3.3%, respectively. Milk protein contents tended to increase by 1.5% in SBMD and CGMD treatments compared to the control. Increasing the dietary protein supplies increased FE in SBMD and CGMD treatments compared to control, whereas, MNE decreased by 10.9%. No effect was observed on DM, N and NEL intakes when SBM was partially replaced with CGM. Consequently, milk yield, milk components’ yield, milk composition and feed efficiency remained unaffected. Contrary to this, MNE decreased by 5% in CGM treatment compared to SBM. There were no dietary treatment effects on blood metabolites including BUN, glucose and TG concentrations, which means neither replacement of SBM nor concentration of protein in the diet affected the blood metabolites profile. There was no change in lactation performance of cows by the partial replacement of SBM with CGM. Therefore, SMB could be partially replaced with CGM with urea without affecting animal performance, and saving the feed cost. Table 6.2: Effects of experimental diets on different parameters Exp.
MP
efficiencies
Δ MP
efficiencies (%)
Δ MY
(kg)
Δ DMI
(kg)
Δ milk
fat (%)
Δ milk
protein (%)
1 0.64 14.3 5.20 0.10 7.80 5.30
2 0.65 11.6 1.20 0.70 3.93 1.50
3 0.68 9.85 1.10 0.20 2.18 1.10
Exp., experiment; MP, metabolizable protein; MY, milk yield; DMI, dry matter intake
Summary
104
In conclusion, balancing Lys to Met ratio at low protein diets and partial replacement of SMB with CGM is a mean to improve the MNE and reduce feed costs. 6.1 Conclusion and Recommendations Diets with low MP supply result in high MNE and better utilization with low levels of BUN. Although there was less milk yield in low protein diets but utilizing efficiency was high. Low protein corn-soy-based diets supplemented with rumen protected Met (HMBi) result in increased utilization of protein and low levels of BUN. Partial replacement of SBM with CGM plus urea showed no change in DMI, milk yield. Milk nitrogen efficiency was slightly decreased in CGM diet as compared to SBM diet. Feed cost could be saved by replacing 35% SBM with CGM provided that RDP is balanced by using NPN sources. Diets should be given with possible lowest protein levels having balanced AA particularly Lys and Met, which should be 3:1. High levels of protein could result into increased emission of gases to the environment. Soybean meal replacement with CGM along with some NPN source results in similar outcomes. First strategy is the best out of three currently tested and it can save money. 6.2 Future Perspectives Studies must be conducted to investigate the effects of further lowering the dietary protein levels without affecting milk production in Holstein cows. It will help to improve the dietary N utilization for milk synthesis. The above-mentioned strategies can also be tried simultaneously for improved protein/N utilization in dairy cows. Lysine can also be tried along with Met to balance the low protein corn-soy-based diets. On the basis of RDP and RUP values, other ingredients can also be tried to partially replace SMB. Availability: Items available for loan: UVAS Library [Call number: 2920-T] (1).
379.
Moringa Oleifera As Feed Additive In Poultry: Influence On Production Efficiency, Meat And Egg Profile
by Shakeel Ahmad (2011-VA-542) | Prof. Dr. Anjum Khalique | Prof. Dr. Talat Naseer Pasha | Dr. Shahid Mehmood.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: Phytogenic feed additives gained considerable interest in the strategic replacement of antibiotic growth promoters (AGP) after European regulations for animal feed production which imposed a complete ban on antibiotics in animal feed used as growth promoters. Natural phytogenic growth promoters modify animal metabolism and gut microbiota population and positively affect the production and growth performance. Research in the animal feed production has established the basis for better feed efficiency, desired carcass traits and manipulation of bioactive compounds in the poultry eggs and meat, which resulted in the idea of functional foods and phytogenic feeds additives. In response to the above challenges a lot of research on aromatic plants, herbs, and spices was conducted in comparison with conventional antibiotic growth promoters. The data were recorded and analysed regarding gut morphology, gut ecology, feed digestibility, nutrients availability and meat and eggs bioactive compounds index of meat and eggs. Moringa oleifera might be used as phytogenic feed additives on the basis of diverse volatile compounds present in its leaves, pods and roots. Moringa belongs to family Moringaceae and is commercially grown in many tropics and subtropics countries globally. Moringa oleifera leaves and pods are very nutritious and retain their nutrients even when dried and converted to leaf powder. They can be utilized in both fresh as well as dried forms. Moringa leaves contain bioactive compounds like β-carotene, Quercetin and selenium in addition to basic nutrients (crude protein, metabolizable energy, ether extract, and ash). Moringa leaf meal contains 29.7, 22.5, 14.7, 4.3, 2.7, 0.26, 10.6% and 7.86Mj/Kg of CP, CF, Ash, EE, Ca, P, NFE and ME respectively. On the basis of above nutritional and bioactive compounds Moringa oleifera leaves meet the needs of a growth promoter and phytogenic feed additive as lot of studies on antimicrobial, anti-coccidial, AGP replacer and feed ingredient attributes have been conducted. Even then, very limited data was available regarding its phytogenic feed additive attributes. On the basis of above addressed issues the below experiments were designed. For this purpose following experiments were planned and executed. In the first experiment, authors took 200 day old broiler chicks and assigned to four dietary treatments in a completely randomized design replicated five times having ten birds each. Four iso-caloric and iso-nitrogenous diets were formulated for both the starter as well as finisher phases of production and four levels (0, 0.5, 1.0 and 1.5%) of Moringa leaf meal (MLM) were used over and above. Data regarding growth performance were collected and subjected to Analysis of Variance (ANOVA) techniques under Completely Randomized Design and means were compared using Duncan’s Multiple Range (DMR) test. Growth was monitored by feed intake, body weight, total gain, FCR and liveability. Feed intake was linearly decreased whereas quadratic response was observed in FCR (P≤0.05). Body weight, total gain and liveability remained same among the treatment groups. Dressing percentage showed quadratic response and highest values was observed in the group offered 0.5% of supplementation, whereas all other parameters including live weight, carcass weight, digestive organs (liver, gizzard) and heart size showed no effect. Bioactive compounds β-carotene, Quercetin and selenium in breast meat samples were lineally increased resulting in higher DPPH values (P≤0.05). Cholesterol, SGPT, creatinine and glucose levels in serum and breast meat samples were linearly decreased resulting in improved animal as well as consumer health. Moisture level of breast meat samples was linearly decreased whereas all other nutrients including Crude protein (CP), Ash, Ether extract (EE) and all minerals were linearly increased with the supplementation level (P≤0.05). Newcastle dis□ease titers remained unchanged while linear increase in IBD titers was observed. In second trial Moringa oleifera pods meal was added in the diet (starter and finisher) with same levels 0, 0.5, 1.0 and 1.5%. Two hundred broiler birds were purchased from commercial market and assigned to four treatments with five replicates and ten birds per replicate. Feed conversion ratio and feed intake was decreased (P≤0.05) with the increase in the supplementation level, whereas total gain was improved. Dressing percentage was negatively affected (P≤0.05), whereas organs weights (liver, hear & gizzard) were improved with the supplementation levels. β-carotene and Quercetin content of broiler breast meat were respectively 0.08μg/100g and 8μg/100g breast meat samples. Cholesterol level was decreased whereas selenium content was significantly increased (P≤0.05) in the treatment groups. Highest DPPH radicals scavenging activity was recorded in the treatment groups. When proximate analysis of meat was done, the moisture, ether extract, crude protein and ash contents were decreased (P≤0.05). Moreover biochemical indicators SGPT, Creatinine, Glucose and cholesterol were recorded significantly lowest (P≤0.05) in the treatment groups. In the third experiment Moringa oleifera leaf meal was used as feed additive in layers diet to its impact on performance, bioactive compounds and nutrient profile of eggs. For this purpose two hundred, fifty weeks (50) old, HyLine W36 layers were purchased from the commercial market and assigned to four treatments in a completely randomized design with five replicates and ten birds per replicate. Four iso-caloric and iso-nitrogenous diets were formulated and Moringa oleifera leaf meal (MLM) was supplemented at 0, 0.5, 1.0 and 1.5% over and above of basal diet. Results of this experiment significantly affected the production. Egg mass, production percentage and FCR were significantly affected whereas total feed intake and egg weight remained unchanged (P≤0.05). Egg shape and yolk index showed a quadratic response, whereas Haugh units and shell thickness was linearly decreased (P≤0.05). Bioactive compounds like β-carotene, Quercetin and Selenium content were enriched up to the levels 4906 and 241 and 56.82 μg/100g of egg yolk respectively (P≤0.05). Linear decrease in the serum biochemical compounds SGPT, Glucose, Creatinine and cholesterol
SUMMARY
143
levels (serum and eggs) at 4th and 6th week of supplementation was recorded (P≤0.05). Antibody titers against Newcastle disease were also significantly improved
(P≤0.05). Nutrients and minerals profile of egg yolk was also significantly changed (P≤0.05). In experiment No 4, effect of Moringa oleifera pods meal was investigated on 200 HyLine W36 layer birds of 50 weeks age. Diets were formulated having same caloric and protein levels and Moringa oleifera pods meal was added at top with the dose levels of 0, 0.5, 1.0 and 1.5% of basal diet. The results of this study showed quadratic response on egg mass and FCR per dozen eggs whereas feed intake, egg weight and production percentage remained same. Haugh unit showed a quadratic response whereas shell thickness was decreased linearly in the treatment groups (P≤0.05). Antioxidants like, β-carotene, Quercetin and Selenium showed same trend as in the previous experiment. Serum biochemical profile (SGPT, Glucose, Creatinine and cholesterol) were improved along with lower cholesterol content in egg yolk (P≤0.05). Proximate analysis of egg yolk showed that moisture and ether extract was decreased whereas CP, Ash and minerals (Na, K, Ca, Mg, P) profile was improved with the supplementation (P≤0.05). Results of all these four experiments support our hypothesis that Moringa oleifera can be a good phytogenic functional feed additive and can even perform at these lower supplementation levels. Moreover it can also be stated that eggs and meat produced from Moringa oleifera leaf and pods supplemented birds may serve as a functional food.
Conclusion
SUMMARY
144
On the basis of above experiments, it can be concluded that Moringa oleifera leaf and pods meal could be used to enhance the growth and production performance of broilers and layer bird along with bioactive compounds, like antioxidants, minerals and vitamins index of meat and eggs which could meet the advance concept of phytogenic feed additives.
Suggestions and recommendations
Outcomes of the present study including positive impact on growth performance, carcass traits, serum biochemical profile, immunity in broilers, and production percentage, egg geometry, and egg quality in layers in addition to enrichment of β-carotene, Quercetin and selenium in meat eggs suggest that Moringa oleifera can be considered as phytogenic feed additive.
Recommendations for the farmers
Keeping in view the above study following recommendation are given for the poultry producers
1. Moringa oleifera on the basis of its availability and nutrient profile can be a good phytogenic feed additive but there are multiple limitations most important are anti-nutritional compounds like saponins, phytosterols and high fibre and ash content in the leaves and pods meal inclusion in the feed.
2. Moringa pods are available once in a year and its availability is scarce so proper storage should be monitored.
3. Depending on the soil composition and climatic condition and varieties the nutrient especially ash content and bioactive compounds differ in its concentration so proper validation of nutrients is required before supplementation.
4. Supplementation is dependent on the nutrient profile so proper protocol and care must be considered while its use.
SUMMARY
145
5. In present study it is suggested that Moringa oleifera could be an option to be used as phytogenic feed additives to meet the condition including ban on AGP, better growth, production performance and safe and healthy meat and eggs.
Suggestions for the researchers
1. In the present study four levels of supplementation were used but future researchers can explore different levels in their studies.
2. Comparative studies with synthetic antioxidants, carotenoids and organic selenium can be designed.
3. Effect on shelf life can be studied in the animal products can be investigated in future studies.
Subsequent effect on acceptability of consumers regarding effect on the health including cholesterol levels, hypertension and cancer diseases can be studied. Availability: Items available for loan: UVAS Library [Call number: 2940-T] (1).
380.
principles of animal nutrition and Feeds
by Banerjee, G. C.
Edition: 1st revised ed.Material type: ; Literary form:
not fiction
Publisher: India: CBS Publishers; 2018Availability: Items available for loan: Pattoki Library [Call number: 636.085 Banerjee 32592 1st 2018 Animal.Nutrition] (1).
381.
Tannins and Phenolics in Animal Nutrition: Chemistry and Challenges
by Amelot, Miguel E. Alonso.
Edition: 1stMaterial type: ; Literary form:
not fiction
Publisher: New York: Nova Science Publishers; 2011Availability: Items available for loan: Pattoki Library [Call number: 636.0852 Amelot 32692 1st 2011 Animal.Nutrition] (2).
382.
Animal feeding and nutrition
by Jurgens, Marshall H.
Edition: 1st ed.Material type: ; Literary form:
not fiction
Publisher: USA: Kendall Hunt; 32691Availability: Items available for loan: Pattoki Library [Call number: 636.085 Jurgens 32691 1st 2012 Animal.Nutrition] (1).
383.
Poultry Feeds and Nutrition
by Schaible,Philips.
Edition: 1st edMaterial type: ; Literary form:
not fiction
Publisher: UK AVI PUBLISHING 1970Availability: Items available for loan: Pattoki Library [Call number: 636.5084 Schaible 9234 1st 1970 Poultry] (1).
384.
Dairy Chemistry and Animal Nutrition
by Rai,M.M.
Edition: 1st ed.Material type: ; Literary form:
not fiction
Publisher: India: Kalyani; 2011Availability: Items available for loan: Pattoki Library [Call number: 636.2142 Rai 50562 1st 2011 Dairy] (2).
385.
Vitamins in animal and human nutrition
by McDowell, L. R.
Edition: 2nd ed.Material type: ; Literary form:
not fiction
Publisher: USA: Iowa State University Press; 2000Availability: Items available for loan: Pattoki Library [Call number: 613.286 McDowell 32896 2nd 2000 H.Nutrition] (1).
386.
Amino acids in animal nutrition
by D'Mello, J. P. Felix.
Edition: 2nd ed.Material type: ; Literary form:
not fiction
Publisher: UK: CABI Publishing; 2003Availability: Items available for loan: Pattoki Library [Call number: 636.0852 D'Mello 32895 2nd 2003 Animal.Nutrition] (1).
387.
Dog Breeding,Whelping and Puppy Care
by England,Gary.
Edition: 1st ed.Material type: ; Literary form:
not fiction
Publisher: UK: WILLEY BLACKWELL; 2013Availability: Items available for loan: Pattoki Library [Call number: 636.70887 England 50338 1st 2013 Theriogenology] (4). Checked out (1).
388.
Nutritional Management of Hospitalized Small Animals
by Chan,Daniel L.
Edition: 1st ed.Material type: ; Literary form:
not fiction
Publisher: Singapore Blackwell, 2015Availability: Items available for loan: Pattoki Library [Call number: 636.0852 Chan 50382 1st 2015 Animal.Nutrition] (1).
389.
Phosphorous and Calcium Utilization and Requirements in Farm Animals
by Vitti,Dorinha.
Edition: 1st ed.Material type: ; Literary form:
not fiction
Publisher: UK CABI, 2010Availability: Items available for loan: Pattoki Library [Call number: 636.0852 Vitti 50381 1st 2010 Animal.Nutrition] (1).
390.
The Encyclopedia of Farm Animal Nutrition
by Fullar,M.F.
Edition: 1st ed.Material type: ; Literary form:
not fiction
Publisher: UK CABI; 2004Availability: Items available for loan: Pattoki Library [Call number: 636.08503 Fuller 50374 1st 2004 Animal.Nutrition] (1).
391.
Plant Phytochemicals in Animal Nutrition
by Abdel Fattah.
Edition: 1st ed.Material type: ; Literary form:
not fiction
Publisher: USA Nova Science; 2011Availability: Items available for loan: Pattoki Library [Call number: 636.085 Fattah 50379 1st 2011 Animal.Nutrition] (1).
392.
Animal Feeding Formulation Economic and Computer Appications
by Pesti,Gene M.
Edition: 1st ed.Material type: ; Literary form:
not fiction
Publisher: USA CHAPMAN & HALL; 1992Availability: Items available for loan: Pattoki Library [Call number: 636.089557 Pesti 50426 1st 1992 A.Nutrition] (1).
393.
The Ruminant animal : digestive physiology and nutrition
by Church, D. C.
Edition: 1st ed.Material type: ; Literary form:
not fiction
Publisher: USA: Availability: Items available for loan: UVAS Library [Call number: 636.208923 Church 2198BB 1st 1988 Physiology] (1).
394.
Handbook of General Animal Nutrition
by Chahal,Udeybir Singh.
Edition: 1st ed.Material type: ; Literary form:
not fiction
Publisher: India: CBS; 2015Availability: Items available for loan: Pattoki Library [Call number: 636.082 Chahal 50563 1st 2015 A.Nutrition] (2).
395.
Advanced Animal Nutrition
by Reddy,DV.
Edition: 1st ed.Material type: Publisher: India: Oxford and IBH 2017Availability: Items available for loan: Pattoki Library [Call number: 636.082 Reddy 50561 1st 2017 A.Nutrition] (1).
396.
Mineral Tolerance of Animals
by National Research Council.
Edition: 2nd ed.Material type: ; Literary form:
not fiction
Publisher: USA National Research Council, 2005Availability: Items available for loan: Pattoki Library [Call number: 636.08959 NRC 50570 2ND 2005 A.Nutrition] (1).
397.
Principles of animal Nutrition
by Reddy,DV.
Edition: 3rd ed.Material type: ; Literary form:
not fiction
Publisher: India: CBS; 2017Availability: Items available for loan: Pattoki Library [Call number: 636.082 Reddy 50564 1st 2017 A.Nutrition] (1).
