301.
Comparative Efficacy Of Xylocaine Hcl And Bupivacaine Hcl For Ophthalmic Anesthesia In Horses
by Muhammad Asad Islam (2012-VA-576) | Dr. Sadaf Aslam | Prof. Dr. Muhammad Arif Khan | Prof. Dr. Muhammad Sarwar Khan.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: Ophthalmic procedures can be performed by many clinicians in horses using local nerve blocks by using local anaesthetics for short duration of action for completion of these procedures. These surgical procedures may involve exclusion of third eyelid, suturing of laceration around eye orbit and tumor which can be caused due to any reason with in time period of thirty minutes. Inner chamber centesis can be done easily by using the above mentioned technique in standing horse.
There are numerous benefits of doing standing surgical methods and avoiding general anaesthesia in horses. As hospitalizing horse may get other infectious diseases from surroundings like colitis and laminitis and also injured it when recovering from general anaesthesia. On the other hand standing surgical procedure reduced such complication by using local anaesthetic for short duration.
Bupivacaine Hcl gave an ideal local eye anaesthesia compare to xylocaine Hcl for standing surgical procedures in horses.
The present study was accomplished to assess the effectiveness of two local eye anaesthetics; xylocaine Hcl and bupivacaine Hcl by two different techniques i.e. retrobulbar technique and auriculopalpebral technique in horses. A total of 12 horses from indoor clinic and S.P.C.A were used in this study. These horses were subjected to two groups’ i.e. Group A and B. Each of these groups was further subdivided into two subgroups i.e. Group AI, AII and BI, BII respectively.
SUMMARY
42
Horses in group A were administered xylocaine Hcl through auriculopalpebral technique and retrobulbar technique. While horses of subgroup AI were given xylocaine Hcl by auriculopalpebral technique and horses in subgroup AII were injected xylocaine Hcl by retrobulbar technique.
Likewise horses in subgroup BI were given bupivacaine Hcl by auriculopalpebral technique, while those of subgroup BII were given bupivacaine Hcl through retrobulbar technique. The efficacy of above mentioned local anaesthetics was compared on the basis of Pattern of induction, Duration of anaesthesia and Recovery Pattern. Presence or absence of reflexes was also noted i.e. Pupillary Light Reflex and Blink reflex.
The data were analyzed through one way analysis of variance (ANOVA). The difference in group’s means was determined by Least Significant Difference (LSD) post-hoc test. A probability level of (P<0.05) was considered as statistically significantly difference. The statistical analysis was performed using statistical package for social science (SPSS) version16. Availability: Items available for loan: UVAS Library [Call number: 2679-T] (1).
302.
Infection Rate And Chemotherapy Of Haemonchus Contortus In Mouflon Sheep
by Majeed Ul Zafar Jaidi (2013-VA-890) | Dr. Waseem Yaqub | Dr. Muhammad Sarwar Khan | Dr. Zia Ullah.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: Mouflon sheep have lovely brown colored short haired coat. Typically it is not the wild animal found in Pakistan but a little population of Mouflon sheep is present in Pakistan in the captive vicinities like private zoo and wild life parks .Their population is countable, 150-200 Mouflon sheep are present. They can be parasitized by many nematodes, one of the most important is Haemonchuscontortus. Adult Haemonchuscontortus found in the abomasum of the animal.Female parasite can lay up to 1500 eggs in a day in mid-Summer July- August and those eggs produce the infective stage L3, which after infestation causes heavy blood loss resulting anemia, weight loss, emaciation and sudden death in acute cases compromising the production and propagation losses in Mouflon sheep.
The Mouflon sheep of various private and public Zoo and Wild Life Parks located in area of District Lahore were included in this study. A total of 100 Mouflon sheep were examined coprologically for the presence of Haemonchuscontortus for the present study. It is difficult to restrain the wild animal, a Dort was used for this purpose keeping in view of this problem about 3 gram of sample were collected early in the morning from the freshly passed feces, for this purpose disposable gloves was used on hands,the samples were collected carefully to avoid soil contamination the sample was placed in self-sealing polythene bags and were transferred to the laboratory in ice pack cooler. The samples were stored in refrigerator at 4°C till analysis.
The fecal samples were analyzed for Haemonchuscontortus eggs using direct smear method and floatation technique, while the egg count were performed by McMaster technique at medicine Laboratory University of Veterinary Animal Sciences Lahore the identification of Haemonchuscontortus was made by using standard procedures. Infection rate was calculated by using formula
Infection rate (%) = No. of infected animals (n)/ total No. of sampled animals (N) × 100
The infection rates were calculated in this study and total Thirty (33) animals were found positive after qualitative and quantitative analysis of fecal samples. The infection rate of Haemonchuscontortus in Mouflon sheep were calculated out of sampled animals which resulted the significant (P < 0.05) infection rate in females as well as in male Mouflon sheep. The infection rate in female Mouflon sheep was 33.82 % and in males it was 31.25 % in males out of positive animals. Similarly The infection rate of H. contortus in Lahore Zoo, Safari Park Lahore and Jallo Park was 29.72, 32.50, 39.13 respectively, and the infection rate of H. contortus in age group of 1-3, 4-6, 7-9 was 39.58, 27.27, 26.31 respectively.
For therapeutic trails, a total of 30 animals positive for nematodes having egg per gram between 1000---2000 were divided into 3 groups A, B, and C each group were comprised of 10 animals. The animal of group A was treated with Albendazole at the dose rate of 10 mg per kg of body weight PO; group B was treated with Levamisole at the rate of 7.5 mg per kg of body weight PO whereas the group C was treated with Pyrentelpamoate at the dose rate of 25 gram PO.The fecal sample of all groups were collected at day 0 (pre-treatment) and then at 3rd day 5th, 7th and 12th (post-treatment). The efficacies of these drugs were assessed on the bases of reduction in egg per gram and calculated as per formula of (Iqbal at al. 2013).
Drug efficacy = {(pre-treatment EPG - post-treatment EPG / pre - treatment EPG)} × 100
The chemotherapy of Haemonchuscontortusin Mouflon Sheep were studied in different 3 treatment groups. Microscopically screened out Haemonchuscontortus positive Mouflon sheep were divided in Three (3) treatment groups T 1, T 2 and T 3 and each group contained Ten (10) positive animals. Faecal samples of animals were examined at day 0 pre-treatment and at days 3, 5, 7 and 12 post treatment. All the treatment groups showed a significant reduction (P < 0.05) in eggs per gram (EPG) at 3rd, 5th, 7th and 12th days after treatment. The maximum reduction in EPG %age was 96.1 % showed by T 1 group treated with single dose of Albendazole at 10 mg/kg body weight at day 7 post treatment while the groups T 2 and T 3 showed maximum reduction of 95.52 % and 93.26 % at day 12th post treatment. Hence Albendazole was the best group found against Haemonchuscontortus at day 7 post treatment among the other two groups of drug used.
Data on Infection rate of Haemonchuscontortus was estimated by Pearson’s chi-square test. For significance whereas data on chemotherapy was analyzed by one way analysis of variance (ANOVA) using SPSS, P< 0.05 were considered significant.
Availability: Items available for loan: UVAS Library [Call number: 2677-T] (1).
303.
Comparative Efficacy Of Ozone And Gentamicin Sulphate On Uterine Infections In Crossbred Dairy Cows
by Muhammad Usman Raza (2014-VA-914) | Prof. Dr. Aneela Zameer Durrani | Mr. Ghazzanffar Ali Chishti | Dr. Muti ur Rehman.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Efficient fertility of lactating dairy cows has always been the doorstep to economically profitable dairy farming. It is mostly agreed that uterine diseases in the cow after parturition have a negative effect on overall reproductive performance. Ozone which is assumed as a very potent oxidant, is one among these alternative techniques. The advantage of using ozone rather than antibiotics is in lowering the incidence of bacterial resistance in consumers of foodstuffs of animal origin, and other advantage is that ozone has no withdrawal period for milk, meat and other products. Ozone breaks through the microorganism (bacteria and germs) cell membrane, and also destroys viruses by diffusing through the protein coat in the nucleic acid core, resulting in damage of the viral nucleic acid.
The study was conducted on 50 animals which were randomly divided in two groups. Both groups were having 25, 25 animals. Group A received Ozone while group B received gentamicin sulphate intra uterine. Uterine lavage was taken twice, once before applying treatment and second after 8 hours of applying treatment. Samples were cultured for bacteriology to detect E.coli, F. necrophorum, A. pyogenes and St. pyogenes. Number of positive cases in Group A was 12, 9, 10 and 7 for E. coli, F. necrophorum, A. pyogenes and St. pyogenes respectively. Number of positive cases in Group B was 10, 8, 11 and 8 for E. coli, F. necrophorum, A. pyogenes and St. pyogenes respectively. After applying certain biochemical tests for each bacteria, bacteria was confirmed.
Difference of the colony forming units of before and after applying treatments for each bacterium in both groups was calculated. This difference was compared with difference of the colony forming units for same bacteria of other group by using Independent 2 samples T-test with the help of Statistical Packages for Social Sciences (SPSS Inc.) for the windows Version 13.3 (Chicago IL, USA).
Results were interpreted on the basis of level of significance. Differences among the groups were considered significant at P < 0.05. E. coli, F.necrophorum and St. pyogenes were highly significant as the P value for group differences was less than 0.05. Group differences among S.pyogenes showed no significance as the P>0.05.
The results showed that ozone is better in efficacy as compared to gentamicin sulphate on uterine infections in cows.
Availability: Items available for loan: UVAS Library [Call number: 2685-T] (1).
304.
Molecular Identification And Treatment Of Theileriosis In Small Ruminants Of Northern Balochistan
by Mir Ahmad Khan (2005-VA-214) | Prof. Dr. Muhammad Arif Khan | Prof. Dr. Muhammad Azam Kakar | Prof. Dr. Muhammad Sarwar Khan | Prof. Dr. Aftab Ahmad Anjum.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: The present study was conducted to investigate the prevalence of Ovine and Caprine Theileriosis in Northern Highlands and Suleiman Mountain Region of Balochistan, Six thickly populated /union councils were included in the study area. Samples were collected from 2870 animals Sheep (n= 2200) and Goats (n= 670) for screening of the disease. The samples were collected and processed in Regional Disease Investigation Laboratories, Department of Livestock and Dairy Development Balochistan, T.B. Sanatorium Hospital Quetta and Center for Vaccinology, Bacteriology, The University of Balochistan, Quetta and Medicine Laboratory, Department of Clinical Medicine and Surgery, The University of Veterinary and Animal Sciences, Lahore. Data revealed 20.82% disease in sheep and 9.70%. in goats. The regional prevalence of theileriosis revealed 19.19% in Northern Highlands and 17.48% in Suleiman Mountain Region Chi-square analysis showed significant difference in the prevalence of disease in sheep and goats. The regional difference was not significantly different between two regions of Northern Balochistan. The comparison among union councils showed significant difference being highest prevalence (22.71%) in union council Kuchlak district Quetta followed by Aghberg (18.42%) and Hanna Urak (15.53%) in Northern highlands and Union Council Zangiwal Jogezai (19.83%) followed by Kach Amaqzai (16.30%) and Sinjavi (15.92%) in SMR. The disease prevalence when compared among 4 different breeds of sheep showed significant difference being highest in Karakul breed (34.62%) followed by Shinwari (24.54%), Bibrik (19.36%) and Harnai (16.40%). The highest prevalence of theileriosis in sheep and goats were observed in Summer season (30.30%) followed by Autumn 19.07%, Spring 14.52% and Winter
SUMMERY
105
7.61%. Chi-square analysis of the data showed significant difference in the prevalence of the disease in different seasons of the year. The disease was also compared in three age groups of sheep and goats. The data showed 22.17% disease in adult animal group above 2 years of age followed by 15.85% in animals between 1-2 year and 7.99% in age group below one year. Statistically significant difference in all age groups was found in chi-square analysis. The sex wise prevalence of theileriosis revealed non-significant difference between male and female sheep and goats. Two different species of Theileria were reported by many researchers causing disease in sheep and goats. The PCR was carried out for the identification of Theileria species affecting sheep and goats in Balochistan. Two species specific sets of primers were designed using 18SRNA gene sequence to identify these two species of Theileria and the distribution among the two species of animals. The genomic DNA of two species of parasite was successfully amplified in positive samples. The assay was proved successful and we recommend for the prevalence surveys for theileriosis in sheep and goats. The data showed that the prevalence of T. lestoquardi was 73.80% in sheep and 69.23% was in goats in the target regions. It was found the T. lestoquardi was highly prevalent and causing theileriosis in small ruminants. The prevalence of T. ovis was 26.19% in sheep and 30.76% in goats respectively in the investigated animals; it was less than T. lestoquardi. It was concluded that both Theileria species were identified and found circulating in small ruminants in the target region of Balochistan. In the study we determined that PCR method based on 18S RNA gene could detect and differentiate T. ovis and T. lestoquardi.
Effect of theileriosis in sheep and goats on hemeto-biochemical parameters were studied included RBCs, Hb%, PCV, Platelets, WBCs, MCV, MCHC, AST, ALT, BUN, Bilirubin and Creatinine. Blood samples were collected from Theileria confirmed, diseased animals (sheep and
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106
goats) along with equal number of healthy animals for comparison. In sheep RBCs, Hb%, PCV, WBCs, MCHC, AST, ALT and Creatinine values showed significant difference when compared with values of healthy animals. Significant (p<0.05) reduction was noted in measurement of RBCs, Hb%, PCV and MCHC whereas, AST, ALT and Creatinine showed significant increase in diseased animals. In goats affected with theileriosis showed significant decrease in RBCs count and Hb%. The values for AST, ALT and Creatinine were found significantly increased in diseased animals when compared with healthy control group of equal number of animals. In present study it was noted that Butalex intra muscularly at the rate of 2.5 mg/kg body weight is quite effective in eliminating the Theileria parasite from the blood of sheep and goats and treatment at the day 10 post treatment. Imizol was also found an effective treatment of theileriosis but less effective than Butalex. Availability: No items available
305.
Small Animal Diagnostic Ultrasound / 3rd ed.
by Mattoon, John S.
Edition: 3rd ed.Material type: ; Literary form:
not fiction
Publisher: Canada: Elsevier; 2015Availability: Items available for loan: UVAS Library [Call number: 636.7089607543 Mattoon 32190 3rd 2015 CMS] (1).
306.
Feline General and Oncological Surgery
by Demetriou, Jackie.
Edition: 1st ed. Material type: ; Literary form:
not fiction
Publisher: China: Elsevier; 2014Availability: Items available for loan: UVAS Library [Call number: 636.80897 Demetriou 32138 1st 2014 CMS] (1).
307.
Manual of Canine and Feline Cardiology
by Smith, JR.
Edition: 5thMaterial type: ; Literary form:
not fiction
Publisher: China: Elsivier; 2016Availability: No items available Checked out (1).
308.
Current Therapy in Avian Medicine and Surgery
by Speer, Brian L.
Edition: 1st ed. Material type: ; Literary form:
not fiction
Publisher: China: Elsevier; 2016Availability: Items available for loan: UVAS Library [Call number: 636.5089 Speer 32137 1st 2016 CMS] (1).
309.
Small Animal Diagnostic Ultrasound / 2nd ed.
by Nyland, Thomas.
Edition: 2nd ed.Material type: ; Literary form:
not fiction
Publisher: UK Elsevier; 2002Availability: No items available Checked out (1).
310.
Clinico-Epidemiological Study Of Multiple Drug Resistant Staphylococcus Aureus From Bovine Mastitis
by Muhammad Abdul Rauf Malik (2015-VA-832) | Dr. Muhammad Ijaz | Dr. Syed Saleem Ahmad | Prof. Dr. Aftab Ahmad Anjum.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: Bovine mastitis is one of the most significant problems of livestock causing huge economic losses in dairy industry of Pakistan. Among other bacterial etiology of bovine mastitis, the Staphylococcus aureus is overwhelming to control and is well-known to cause subclinical and contagious mastitis. Methicillin resistant Staphylococcus aureus is our prevailing field issue. In view of the economic importance of Staphylococcus aureus mastitis, the current project was designed to study the Clinico-epidemiology of multiple drug resistant Staphylococcus aureus in bovine mastitis.
A total number of 900 milk samples (n=450 cattle, n=450 buffalo) were collected from Faisalabad district of Punjab, The collected samples were processed in laboratory of Microbiology and Medicine, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, Lahore. Primarily, screening of subclinical mastitis was done by Surf Field Mastitis Test (SFMT). Milk samples were spread out primarily on blood agar to rule out possibility of loss of later growth. Colonies with round and golden color characteristic were put to sub-culturing on Mannitol salt agar as differential and selective medium. The morphological and microscopic clarification was done under microscope using Gram’s staining technique. Various biochemical tests including coagulase and catalase were applied.
Prevalence of subclinical mastitis was found 55% (495/900), however significant differences were found among different tehsils of district Faisalabad. The prevalence of subclinical mastitis in cattle from district Faisalabad was found 54% (243/450) that upon comparison between different cities presented significant difference. While buffaloes presented 56% of subclinical mastitis. Comparisons of subclinical mastitis among different tehsils were
Summary
86
found significant.
Among quarter based prevalence of subclinical mastitis and prevalence of blocked quarters the number of blocked quarters were found 5.58% (201/3600) with highest percentage of blocked quarter noted in case of front right followed by rear right , front left , and rear left with 6.89, 6.56, 4.67, and 4.22%, respectively. However, the quarter based prevalence was found 32% (1088/3399) from bovine. The association of bovine subclinical mastitis with different risk factors presented significant association with few exceptions. Age, parity and body health status of animal were found non- significant with prevalence of mastitis. The breed and open rearing system presented significant relation with chances of mastitis. Chi-square test was used to statistically correlate the risk factors and prevalence of subclinical mastitis. P-value less than 0.05 was considered as significant.
Molecular confirmation of S. aureus was done by using coag gene through PCR technique. The S. aureus which were isolated from milk samples were put to antibiotic (oxacillin) sensitivity test for estimation of prevalence of methicillin resistant S. aureus from the bovine mastitis. Molecular identification of mec-A gene in staphylococcus aureus was done through PCR. The PCR confirmed methicillin resistant S. aureus isolates from cattle (n=20) and buffalo (n=20) were tested for their in-vitro drug response. However, Ciprofloxcin, Moxifloxacine, Linezolid, and Trimethoprim + Sulphamethoxazole were found 100% effective against multiple drug resistant Staphylococcus aureus and Levofloxacin showed 90% efficacy in bovine. While Oxytetracycline, Tylosin, Gentamycin, Amikacin, Vancomycin, and Fusidic acid were also found sensitive moderately except cefoxitin which was responsible for 100% resistane in bovine. Gentamycin was found to much more effective in buffaloes rather than cattle.
Summary
87
The study provided current status of Staphylococcus aureus infection with higher percentage in bovine mastitis. The prevalence of mecA gene revealed variation in methicillin resistant Staphylococcus aureus. In-vitro drug trial provided effective treatment possibilities against multiple drug resistance Staphylococcus aureus. Availability: Items available for loan: UVAS Library [Call number: 2815-T] (1).
311.
Comparative Efficacy Of Ketamine-Xylazine-Tramadol Combination With Ketamine-Xylazine-Ketorolac Tromethamine In Dogs
by Ameer Hamza Rabbani (2009-VA-58) | Dr. Hamad Bin Rashid | Prof. Dr. Muhammad Arif Khan | Dr. Mamoona Chaudhry.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: The study was designed on the premise that use of Ketamine-Xylazine-Tramadol combination was more efficacious in providing better depth of anesthesia and postoperative analgesia than the combination of Ketamine-Xylazine-Ketorolac tromethamine.
Two separate combinations of anesthetic cocktails i.e. ketamine-xylazine-tramadol and ketamine-xylazine-Ketorolac tromethamine were tested for their comparative efficacies. A total of 8 mongrel adult clinically healthy male dogs between 1 to 2 years of age which were presented to clinic for castration, were managed in to two groups comprising 4 (n=4) individuals in each. These groups were designated as group A and B. General and clinical examination of the animal, LFT and RFT were performed prior to surgery. Atropine was administered at dose rate of 0.04mg/kg SC twenty minutes prior to the surgery. Both anesthetic regiments were used respectively in either groups and their efficacy was tested based upon the depth of anesthesia and postoperative analgesia.
In case of group A ketamine was used at a dose rate of 6mg/kg, xylazine at 0.5mg/kg and tramadol at a dose rate of 2mg/kg. These anesthetic agents were administered intramuscularly. The time it took for onset of sedation and depth of anesthesia was monitored, while presence of ocular, oropharyngeal, and anal sphincter reflexes indicated induction, depth, maintenance and recovery from sedation. In case of group B, ketamine was used at a dose rate of 6mg/kg, xylazine at 0.5mg/kg and ketorolac tromethamine at a dose rate of 0.5mg/kg. These anesthetic agents were administered intramuscularly. The time it took for onset of sedation and depth of anesthesia were monitored, while presence of ocular, oropharyngeal, and anal sphincter reflexes indicated induction, depth, maintenance and recovery from sedation. Surgical procedure for castration was
SUMMARY
58
performed as surgical plane of anesthesia was acquired. Postoperatively, Intramuscular antibiotics were injected for 5 days to avoid secondary bacterial infections to wounds. Cleaning of the wound with good antiseptic solution and excellent hygienic measures were adopted to minimize infection. Balanced diet was given to improve health of animals. LFT and RFT were performed preoperatively (baseline), and one hour postoperatively in each dog. The measurements were analyzed as change from baseline in each dog.
The results were statistically analyzed using t-test with significant level 0.05% (p≤0.05), using statistical analysis software Statistica. The results of temperature, pulse and respiration were statistically analyzed using t-test with significant level 0.05% (p≤0.05). Observations pertaining to depth and quality of sedation were analyzed by using t-test with statistical analysis software Statistica. Statistical significance was assigned at p≤0.05.
This study was helpful in determining efficacy of tramadol an opioid against ketorolac tromethamine which is an NSAID. This study emphasized upon discovering the most efficacious adjuvant to anesthetic combination of ketamine and xylazine. This study aimed to reduce the dose of xylazine without compromising the anesthetic effect of the combination while mitigating its side effects as well. This study provided us with a viable analgesic agent which may be administered peri-operatively to provide analgesia during immediate post-surgery period. Furthermore magnitude of improvement in depth of anesthesia helped determine an effective and cheap adjuvant to ketamine-xylazine combination. Availability: Items available for loan: UVAS Library [Call number: 2807-T] (1).
312.
Development Of A Cost-Effective Serodiagnostic Assay For Peste Des Petits Ruminants (PPR)
by Tahira Hanif (2015-VA-1060) | Dr.Jawaria Ali Khan | Dr.Aamer Bin Zahur | Dr. Muhammad Avais | Prof. Dr. Aftab Ahmad Anjum.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: Peste des petits ruminant (PPR) is a highly contagious newly developing disease of Small ruminants (sheep and goats). Currently the poor small ruminant’s farmers in Pakistan are facing huge economic losses due to PPR virus. In Pakistan PPR causes economic losses of Rs. 20.5 billion annually. The objectives of present study were to develop a cost effective sero-diagnostic assay for PPR (active haemagglutination inhibition and passive haemagglutination inhibition) and determination of comparative efficacy of active and passive haemagglutination inhibition assay (HI and PHA respectively) for detection of PPR virus infection.
In the present study, n= 300 sera samples were collected from sheep and goats during the (15 Februry 2016 to 2 January 2017).The serum samples were collected from kotli AJK :20(8 goats and 12 sheep),from gilgit:30 serum (20 goats and 10 sheep),from mansehra:22 serum (13 goats and 9 sheep),from mithi:112 (60 goats and 52 sheep) and 116 serum samples (88goats and 28 sheep) from Dhera ghazi khan.None of the animal was known to have been vaccinated against PPR previously or at the time of sampling.
These samples were collected from animals showed symptoms of PPR suggestive of PPR disease as well as from healthy animals. The sera were transferred into sterile tubes and were preserved on ice packs while shifting to the laboratory. PPR virus isolate was originally isolated from an outbreak in Taxila village, district Rawalpindi, the isolate was attenuated serially onto the Vero cell lines up to 20 passages. After, which antigen was titrated using a micotiter haemagglutination (HA) test with chicken RBCs and stored at -70◦c until use as a PPR antigen in a HI test.
In this study Active haemagglutination inhibition (HI) and passive haemagglutination inhibition were developed. The Haemagglutination Assay was standardized by different factors i.e. diluents, Temperature of incubation, Time of Incubation and concentration of Chicken R.B.C̓s. An additional test passive haemagglutination inhibition was performed to check the comparative efficacy of Active and Passive haemagglutination inhibition. In passive haemagglutination inhibition tanned sensitized cells remains effective due to their long effective life when stored at 4̊c and its makes an ideal test for diagnosis of PPR. Newly developed assays were compared against cELISA for PPR using kappa statistics and diagnostic sensitivity and specificity were determined.
The results of both assays were compared with results of competitive enzyme linked immunosorbent assay. In this study cELISA was considered as golden standard. The relative sensitivity and specificity of Active haemagglutination inhibition is 94.9% and 97.9% respectively. (Kappa 0.9264). However the sensitivity and specificity of Passive haemagglutination inhibition is 91.1% and 95.0% respectively.(kappa 0.8595). This study describes the serological detection of PPR virus by Active haemagglutination and passive haemagglutination inhibition (HI and PHA respectively). It was also concluded the comparative efficacy of (PHA and HI) that Active haemagglutination inhibition is more reliable technique than passive haemagglutination inhibition assay for the diagnosis of PPR disease in small ruminants (sheep and goats). Availability: Items available for loan: UVAS Library [Call number: 2816-T] (1).
313.
Comparative Efficacy of Different Surgical Techniques and Suturing Patterns for Cystotomy in Rabbits
by Uroosa Anjum (2015-VA-601) | Prof. Dr. Muhammad Arif Khan | Dr. Sadaf Aslam | Dr. Raheela Akhtar.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: CD Corrupt. Availability: Items available for loan: UVAS Library [Call number: 2814-T] (1).
314.
Comparative Efficacy Of Surgical And Chemical Methods Of Neutering In Toms
by Zubda Rehman (2015-VA-600) | Dr. Ayesha Safdar Choudhary | Dr. Hammad Bin Rashid | Dr.Arfan Ahmed.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: Sterilization of animals is the most common veterinary practice to control overpopulation and to avoid the transmissible diseases in humans and other animals. This technique also helps to eliminate animals with undesirable characters. This procedure also helps to change the behavior of animals and keep them calm. Castration is carried out to prevent prostatic hyperplasia and mammary tumors etc. There are different methods for sterilization of male animals.
This study was designed to compare the surgical and chemical sterilization. For this purpose 12 free roaming tom cats were used. These cats were divided into group A, group B and group C each having (n=4). Animals of group A were surgically neutered, animals of group B were chemically sterilized with intratesticular injection of 20%calcium chloride and animals of group C were kept as control and injected normal saline intratesticularly.
Chemical sterilization is better than surgical sterilization. Postoperative complications, Serum testosterone level and histopathological examination was evaluated. These parameters were studied for 30 days. Data collected from this study design was evaluated by analysis of Variance. Results of data showed that there was no noteworthy difference in both procedure of sterilization.
From this study we reached the conclusion thatchemical neutering is most preferable than surgical neutering as it is lesser time consuming and cost effective with lesser postoperative complications. Chemical neutering can be easily practiced on large scale to control overpopulation.
. Availability: Items available for loan: UVAS Library [Call number: 2813-T] (1).
315.
Clinico-Theraputic Trial Of Grain Overload In Cattle
by Muhammad Mughees (20015-VA-436) | Dr. Jawaria Ali Khan | Dr. Syed Saleem Ahmed | Dr. Arfan Ahmed .
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: Ruminants are adapted to digest and metabolize predominantly forage diets; however, growth rates and milk production are increased substantially when ruminants consume high grain diets. One consequence of feeding excessive amounts of rapidly fermentable carbohydrates in conjunction with inadequate fiber to ruminants is subacute ruminal acidosis, which is characterized by periods of low ruminal pH that resolve without treatment and is rarely diagnosed. Dairy cows, feedlot cattle, and feedlot sheep are at risk of developing this condition.
The present study was performed in district Kasur in University of Veterinary and Animal Sciences Patoki campus and adjacent areas. Clinical examination of 35 cattle of any breed, age and sex with the history of grain overload/carbohydrate engorgement and showing the sign of Distended rumen, Subnormal temperature, Diarrhea, Depression, Dehydration, Inactivity, Weakness and Anorexia. A total number of 30 animals suffering from lactic acidosis were observed. The ruminal fluid and blood of the affected animals were collected pre-treatment as well as post-treatment to determine their pH values and serum lactate. After processing the diseased animals, treatment trials were run.
A total number of 35 animals were included in this study. They were divided into four groups as A, B, C, and control group. Group A, B and C had 10 diseased animal in each while control group had 5 healthy animals for the reference values. Sodium bi carbonate was given as a treatment to Group A animals at a dose rate of 1g/kg of body weight orally once, Magnesium hydroxide was given as a treatment to Group B animals at a dose rate of 500/450kg of body weight orally once and a combination of Ginger and Ajwain was as a treatment to Group C animals at a dose rate of 100g + 100g orally once. No treatment was given to control group of 5 healthy animals. Ruminal fluid samples and blood samples of all animals were collected for pH and serum lactate values.
Grain engorgement is the managmental disease of cattle in district Kasur.A survey was conducted for a period of 6 months. Convenient sampling was adopted. 5ml of whole blood was collected into test tube from jugular vein using 16 gauge needle and was allowed to clot at room temperature for 1hr to obtain serum.
Serum pH was measured by using vide range of pH indicator paper and pH meter. The ruminal fluid was collected through stomach tube. Ruminal fluid pH wasmeasured by using pH indicator paper and pH meter. A detail predesigned questionnaire was developed for data collection according to inclusion criteria i.e. age, breed, sex, history, severity of signs, management system and vaccination etc.
Data was analyzed using one way ANOVA technique. After this study we are able to know and reduce the major causes & risk factors associated with grain engorgement in large Ruminants.We are able to adopt the different protocols for diagnosis and treatment of grain engorgement.
Availability: Items available for loan: UVAS Library [Call number: 2812-T] (1).
316.
Effect Of Probiotics On Growth Rate And Occurrence Of Diarrhea In Neonatal Holstein Friesian Calves
by Muhammad Ayaz Ahmad (2010-VA-236) | Dr. Muhammad Hassan Saleem | Dr. Muhammad Avais | Dr. Arfan Ahmad.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: Dairy sector commercialization is growing in Pakistan. The import of Holstein cows has been dramatically increased from last decade. Calf mortality is the major constraint in the field of livestock. Calves are considered as the future of dairy farm and mainly responsible for the replacement of stock. Certain diseases cause calf mortality among which bacterial infections carry prime importance.Supplementation of probiotics in milk will reduce the occurrence of diarrhea and increase the body weight, growth rate and average daily gain in Holstein Friesian calves.Holstein Friesian calves (n=60) will be taken from their dams at six days of age and all calves on the basis of initial weight and sex will be selected for the trial. There will be (n=40) calves in the probiotic group and (n=20) calves in the control group.Each group will be further sub-divided into two sub-groups i.e. (n=20) in Group B and (n=25) in Group C. The initial average weight of the calves will be determinedin the two groups. The health status of the calves will be monitored daily with particular attention paid to the occurrence of diarrhea. The actual trial period will start at weaning (day 0) when the calves will be six days old and will be stopped 56 days later. All calves in probiotic group will be offered milk containing probiotic (Calf PRE RD, TechMix, LLCTM, USA) at the dose rate of 4gm per calf per day. The health status, body weight, average daily weight gain and occurrence of diarrhea will be monitored on daily basis. All calves will be given a diarrhea score according to the following scale: 0 = firm, no signs of diarrhea, 1 = soft, slightly loose feacal consistency and 2 = liquid, very loose feacal consistency(Jatkauskas et al., 2010). For each calf the daily scores and the number of days with liquid feaces (score 2) will be summed into an index of the severity of the diarrhea.
Data on occurrence of diarrhea will be compared among groups by using Chi-square (χ2) test whereas data on growth rate, body weight and average daily gain will be measured using t-test. All data will be analyzed using SPSS (statistical package for social sciences),P< 0.05 will be considered significant.
The current study will help in reducing the occurrence of diarrheain Holstein Friesian calves. Further, it will also assist in finding out the efficacy of probiotics supplementation in reducing the incidence of calf mortality.
Availability: Items available for loan: UVAS Library [Call number: 2811-T] (1).
317.
Prevalence, Associated Risk Factors And Treatment Of Toxocariasis In Small Holders’ Buffalo Calves
by Muhammad Qasim Sharif (2007-VA-488) | Dr. Muhammad Avais | Dr. Syed Saleem Ahmad | Dr. Aijaz Ali Channa.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Present study was designed to evaluate the prevalence of Toxocariasis in buffalo calves in outskirts of Lahore. For this 5g fecal samples from 369 buffalo calves were collected directly from the rectum to avoid the soil contamination. For this purpose, buffalo calves between 1 to 20 weeks of age were selected. Data regarding each calf and possible associated risk factor was entered in data capture form. The data capture form included information on species, sex, breed, age, physical condition, weaning time, fecal score, feeding, housing and management etc. After collection the fecal samples were immediately transferred to ice box for transportation to Medicine Laboratory, Department of Clinical Medicine and Surgery, University of Veterinary and Animal Sciences, Lahore. The fecal samples were analyzed by Direct Smear Method as described by Souls by (1982).
Positive samples for T. vitulorum were further subjected to McMaster Technique for Egg per gram (EPG) count.
A total of twenty-four (n=24) calves which were detected as positive for Toxocara. Vitulorum, were randomly selected and divided into 4 groups i.e. Group A, B, C and D, with each group having six calves. Calves in group A were treated with Albendazole at the dose rate of 10 mg/ kg of body weight via oral route while the calves in group B were given Levamisole at dose rate of 8 mg/kg of body weight orally. The animals in group C were treated with Doramectin at 0.2 mg/ kg of body weight via sub-cutaneous route whereas calves in group D were given Chenopodium album at the dose rate of 2g/kg of body weight. All the treatments were given once. The fecal samples from calves in each group were collected at days 0 (pre-treatment) and then at day 4, 7, 14, and 21 (post-treatment) for EPG count through modified McMaster technique. Efficacy of particular treatment was calculated on the basis of fecal egg count reduction test (FECRT).
Data on prevalence was analyzed using chi square test while Odd Ratio (OR) was determined for association of T. vitulorum with different risk factors. The data on EPG at various days was analyzed through two-way analysis of variance (ANOVA). Statistical analysis was performed by using SPSS version 20, at a significance level of 5%.
Out of total 369 buffalo calves, 87 (23.58%). buffalo calves were found to excrete T. vitulorum eggs in their feces. While 212 (76.42 %) buffalo calves were found normal with no fecal egg excretion. 23 calves (26.44 %) with age of 1-4 weeks, 37 calves (42.53 %) with age of 5-10 weeks and 27 calves (31.03 %) were found positive. Percentages of male and female infected calves were 25.79 % and 21.23 % respectively. Number of non-infected calves in healthy and sick animals were 236 (80.55 %) and 54 (71.05 %) respectively. While infected calves in healthy and sick animals were 57 (19.45 %) and 22 (28.95 %). Numbers of sick and healthy mothers of calves were 54 and 315 respectively. 13 calves (24.07%) were infected with T. vitulorum infestation whom mother was sick, while 67 calves (21.27%) were infected with Toxocaravitulorum whom mother was healthy. 77 calves had diarrhea while remaining 292 calves did not have. 28 calves (36.36%) out of 77 (calves suffered from diarrhea) were found as positive for T. vitulorum infestations, while 62 calves (21.23%) out of 292 calves (did not suffer from diarrhea) were infected with T.vitulorum. 280 calves (75.88%) had normal body coat, while 89 calves (24.12%) had abnormal body coat. Number of calves infected with T. vitulorum were 27 (30.34%) and 58 (20.71%), in calves with abnormal and normal body coat respectively.
While after treatment trial it was concluded that Albendazole and Levamisole had effectively reduced the eggs per gram in effected calves. Albendazole eliminate the fecal eggs up to 100 % till day 14. While, Levamisole, Doramectin did the same on day 21. While oral chenopodium album have no significant effect. So it can be concluded that Albendazole can be used in field where there is chances of outbreak of Toxocariasis in calves especially in buffalo calves.
Availability: Items available for loan: UVAS Library [Call number: 2738-T] (1).
318.
A Study Of Risk Factors And Chemotherapy Of Patent Stongyloides Westeri Infection In Foals In Lahore
by Saima Batool (2015-VA-837) | Dr. Jawaria Ali khan | Dr. Muhammad Avais | Dr. Arfan Ahmed.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: Data is available on prevalenc of strongyloideswesteri in foals which cause diarrhea and its allopathic treatment is also available but there is no data available on herbal treatment of s. westeri and best protocol of allopathic drugs. So the purpose of the study is to study the incidence of S. westeri in foals. The risk factors associated with S. westeri and to develop the best treatment protocol for S. Westeri. The hypothesis of this study is; Identification of risk factors that was associated with S. westeri and efficacy of Allopathic verse herbal drugs was determined against S. westeri. Fecal samples were collected from foals. Gloves were lubricated with lubricating material before the sampling. Sample will be collected directly from rectum of the foals. The fecal material was collected in the properly labeled plastic jar. The samples were processed and examined in the Medicine Laboratory, University of Veterinary and Animal Sciences Lahore. Direct smear examination was carried out for the of S. westerieggs under microscope. For quantitative analysis, Mac Master Technique was used. The positive foals were divided into three groups A, B and C to check the efficacy of drugs on S. westeri. These groups were going through to chemotherapeutic trials with different groups of medicines including herbal and allopathic. Herbal medicine was Ginger (zingiberofficinale) and allopathic were oxibendazoleand ivermectin. Milk samples were also collected from newly parturated mares then evaluation and biometery of larvae was also done just to study the risk factors that transfer larvae from dam to foal through colostrum. Comparison and Identification of eggs and larvae in milk along with treatment effect were analyzed by Chi Square method using the SPSS software 22 version. The data obtained from this study revealed that:
The main source of S. westeri infection was milk of dams. The larva of S. westeri passed through the dam’s milk to foals.
The prevalence of S. westeri was strongly associated with the risk factor such age, colostrum intake, species, breed, dite, environment, water supply and deworming treatments.
Vaccination had no effect on prevalence of S. westeri.
Allopathic drugs are more efficient against S. westeri infection in foals as compared the herbal drugs.
The efficacy of Ivermectin was more as compared to Oxibendazole.
Availability: Items available for loan: UVAS Library [Call number: 2817-T] (1).
319.
Fundamentals of small animal surgery / 2nd ed.
by Mann, F. A | Constantinescu, Gheorghe M | Yoon, Hun-Young.
Edition: 2nd ed.Material type: ; Format:
print
; Literary form:
not fiction
Publisher: Malaysia: Wiley Blackwell; 2011Availability: Items available for loan: UVAS Library [Call number: 636.0897 Mann 32319 2nd 2011 CMS] (1).
320.
Medical Mycology : Current Trends and Future Prospects
by Abyaneh - M. Razzaghi.
Edition: 1st ed.Material type: ; Literary form:
not fiction
Publisher: USA: CRC Press; 2016Availability: Items available for loan: UVAS Library [Call number: 616.969 Abyaneh 32312 1st 2016 CMS] (1).
321.
Farm Animal Surgery / 2nd ed.
by Susan L. Fubini | Norm G. Ducharme.
Edition: 2nd ed.Material type: ; Literary form:
not fiction
Publisher: China: Elsevier, 2017Availability: Items available for loan: Pattoki Library [Call number: 636.0897 Fubini 50413 2nd 2017 CMS] (2), UVAS Library [Call number: 636.0897 Fubini 32308 2nd 2017 CMS] (1).
322.
Complications in Small Animal Surgery
by Griffon, Dominique | Hamaide, Annick.
Edition: 1st ed.Material type: ; Literary form:
not fiction
Publisher: Singapore: Wiley Blackwell; 2016Availability: Items available for loan: UVAS Library [Call number: 636.0897 Griffon 32310 1st 2016 CMS] (1).
323.
Veterinary Medicine: Prevention, Diagnosis and Treatment of Diseases in Animals
by Santoro, Andrea.
Edition: 1st ed.Material type: ; Literary form:
not fiction
Publisher: New York: Syrawood; 2016Availability: Items available for loan: UVAS Library [Call number: 636.089 Santoro 32334 1st 2016 CMS] (1).
324.
Color Atlas of Small Animal Dermatology / 2nd ed.
by Wilkinson, George T | Harvey, Richard G.
Edition: 2nd ed.Material type: ; Literary form:
not fiction
Publisher: Spain: Mosby; 1999Availability: Items available for loan: UVAS Library [Call number: 636.708965 Wilkinson 32331 2nd 1999 CMS] (1).
325.
Treatment Of Long Oblique Tibial Fracture With Intramedullary Pin Alone And Full Cerclage Wires In Cats
by Syed Muhammad Faheem Ahmed (2010-VA-147) | Dr. Ayesha Safdar | Prof. Dr. Muhammad Arif Khan | Dr. Muhammad Zahid Tahir.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: Fracture of long bone in animals is a challenging problem these days. Leg fractures in cats are quite common problem. Fractures treated with biomechanically sound fixation and proper attention to soft tissues will be most likely to heal with a functional outcome. However, osteomyelitis, bone sequestration, and joint ankylosis still continue to be major factors in inhibiting healing and bone functionality.
This research project was designed with the aim to test the effectiveness and accuracy of three sets of bone splintage devices in healing long oblique mid-shaft tibial fractures in cats, with minimal side effects and stress to the patient.
For the study total twelve adult cats as and when presented at Pet Centre, University of Veterinary & Animal Sciences Lahore, and Saleem Veterinary Clinic and petshop Lahore, having long oblique fracture of tibia irrespective of breed, sex and considering almost similar age group, both stray and domesticated were selected. These cats were divided into three groups i.e groups A, B and C comprising of four cats in each group, and designated as 1, 2 3 and 4 respectively. They were admitted as clinical trial patients and were kept in separate cat cages at Saleem Veterinary Clinic until the completion of the study. Specific tagged collars were applied on all the cats. They were kept under comfortable environment and on a standard commercially available cat feed diet with ad labitum access to water. All the cats of three groups, having long oblique tibial fracture were treated with 3 separate techniques for comparison. In group A, fracture was reduced and immobilized with intramedullary pinning. In group B, fracture was treated with full cerclage wires alone while cats of group C were treated with intramedullary pin and cerclage wires. Thus comparative fracture healing and efficacy of individual techniques in their respective cases were evaluated.
Subsequent comparative parameters were studied which included physical examination of wound, lameness grading, radiological scoring for callus formation, fracture line union, fracture alignment as well as callus remodelling. The collected data regarding study parameters were illustrated by descriptive statistics and non-parametric analysis of variance (Kruskal Walis Test) using IBM SPSS Statistics version 20.
Conclusion:
Therefore, as advocated by the results of this study, intramedullary pinning (preferably threaded) with at least 2 - 3 full cerclage wiring was a better choice for the correction of a long oblique mid-shaft tibial fracture in cats. The combination of both these techniques together give a strong backbone for bone healing to take place with minimum amount of stress to the bone fragments and also to counteract different forces acting on the fracture site.Both these techniques were easy, required little soft tissue manipulation, were cost-effective, required less equipment and could easily be applied by an average practitioner.
Availability: Items available for loan: UVAS Library [Call number: 2891-T] (1).
326.
Epidemiology, Molecular Detection, Zoonotic Potential, Heamatology And Chemotherapy Of Cryptosporidiosis In Small Ruminants In Southern Khyber Pakhtunkhwa, Pakistan
by Naimat Ullah Khan (2011-VA-516) | Dr. Muhammad Hassan Saleem | Prof. Dr. Aneela Zameer Durrani | Dr. Nisar Ahmad.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: Cryptosporidiosis is one of the most important parasitic enteric protozoan infection affecting all vertebrates. The current study was designed to determine the percent prevalence of cryptosporidiosis in small ruminants and humans along with associated risk factors.
An overall highest percent prevalence of cryptosporidiosis recorded in all four categories of small ruminants were 27.22%, 20.56%, 18.33% and 12.22% in lambs, kids, Sheep and goats respectively.
In the current study, 21.55%, 18.33% and15% prevalence of the Cryptosporidium infection was recorded in sheep in District Kohat, Bannu and Lakki Marwat respectively. In the present study, the highest month wise percent prevalence in sheep, was observed in the month of August (36.66%) followed by April (26.66%), June (26.66%), May, July and September (23.33%), February (10.66%), March (10%), November (10%) while the lowest percent prevalence was observed in the month of December and January (6.66%). In sheep, season wise percent prevalence was also studied where highest prevalence was recorded in summer and autumn season (23.33%), followed by spring (20%) while the lowest percent prevalence was found in the winter season (10%). In sheep, age wise percent prevalence was also studied where highest percent prevalence was found at the age of 1 year (22.38%) followed by 1-2 years (18.03%) while the lowest at the age of 2-3 years (13.46%). In sheep, sex wise percent prevalence was also documented where highest percent prevalence was recorded in female (18.80%) followed by male (17.02%) where lowest percent prevalence was recorded.
In goats, the percent prevalence of the Cryptosporidium infection was also studied in three selected areas where recorded 6.66%, 11.66% and 18.3% prevalence in District Bannu, Lakki Marwat and Kohat respectively. Similarly, in goats, overall highest month wise percent
Summary
154
prevalence was recorded in the month of August (30%), followed by July (23.33%), June (20%), May (16.66%), March and September (13.33%), April and November (10%), January, February and October (3.33%) while the lowest percent prevalence was recorded in December (0%). In the current study, the season wise prevalence was also studied in goats where highest percent prevalence was recorded in the summer season (20.83%), followed by spring (13.33%), autumn (11.66%) while the lowest prevalence was observed in winter season (3.33%). The highest age wise percent prevalence was recorded at the age of 1 year (18.58%) followed by 1-2 years (10.20%) while the lowest at the age of 2-3 years or above (5.95%). According to the sex wise percent prevalence, the highest percent prevalence was recorded in male (12.30%) while the lowest in females (12.17%).
The overall highest percent prevalence of the Cryptosporidium infection was also recorded in lambs in three areas where 33.33%, 25% and 23.33% prevalence was recorded in Kohat, Lakki Marwat and Bannu respectively. The highest month wise percent prevalence was recorded in the month of August (46.6%), followed by other months of the year such as July (40%), April, May and June (30%), September and October (26.66%), November and January (20%) while the lowest in the month of February and December (16.66%) in lambs. The Season wise percent prevalence was recorded in lambs where highest percent prevalence was recorded in summer season (36.66 %), followed by spring and autumn (26.66%) while the lowest in winter season (18.33%). According to the age wise percent prevalence in lambs, the highest prevalence was recorded at the age of 1-15 days (38.09%) followed by 16-30 days (29.41%) while the lowest at the age of 31-60 days or above (15.15%). In lambs, the highest sex wise percent prevalence was recorded in females (31.18%) while the lowest percent prevalence was observed in males (22.98%).
Summary
155
In kids, overall highest percent prevalence was 20.55% recorded in three selected districts where the highest prevalence was recorded in District Kohat (23.33%), followed by District Bannu (20%) while the lowest in District Lakki Marwat (18.33%). In kids the month wise percent prevalence was also studied where the highest percent prevalence was recorded in May and August (33.33%), followed by June, July and September (26.66%), March, April and October (20%), November and December (13.33%) while the lowest percent prevalence was recorded in the month of the January (6.66%). The Season wise percent prevalence was also recorded in kids, where the highest percent prevalence was observed in the summer season (30%), followed by autumn (23.33%), spring (20%) while the lowest prevalence was recorded in winter season (10%). The highest age wise percent prevalence in kids was also recorded at the age of ≤1-15 days (33.92%), followed by 16-30 days (15.38%) while the lowest at the age of ≥31-60 days or above (13.55%). Sex wise percent prevalence was also determined in goat kids where, the highest percent prevalence was recorded in female (20.98%) followed by male kids (19.19%).
To conduct molecular study, 360 fecal samples of sheep were analyzed for presence of the Cryptosporidium oocysts through simple microscopic method first then confirmed by PCR. DNA was extracted with the help of DNA extraction kit (Made in USA, GFC vivantis). The targeted gene of parasite was 18s rRNA which result in amplification of a segment of genomic DNA at 435 bp. The following primers sequence was used for Forward primer: (5-AAGCTCGTAGTTGGATTTCTG- and reverse primers (5-TAAGGTGCTGAAGGAGTAAGG-3. An overall molecular percent prevalence of the Cryptosporidium infection was 24.99% in sheep in three selected zones of southern KPK. The highest molecular percent prevalence was 31.66%, 25% and 18.33% in District Kohat, Bannu and Lakki Marwat respectively.
Summary
156
The highest season wise molecular percent prevalence was also recorded where the highest percent prevalence was recorded in the summer (33.33%), followed by autumn (30%), spring (26.66%) while the lowest in the winter season (13.33%). Molecular percent prevalence was higher in females (27.08%) than male (25.53%). On the basis of environmental factors, overall the highest percent prevalence was recorded in the month of August where highest ambient temperature, relative humidity and heavy rain fall was recorded.
To find out Zoonotic aspect of the Cryptosporidium infection, the overall highest percent prevalence was recorded in children (16.66%), followed by adults (5.55%).
The highest percent prevalence was recorded in diarrhoeic children where direct contact with small ruminants was observed while the lowest prevalence was recorded in those children where no direct or indirect contact was observed.
To conduct the therapeutic trials, a total of 50 goats were selected of the same weight and age that were naturally infected by Cryptosporidium under field conditions. All the goats were placed under same feeding and management conditions and randomly divided into five groups such as A, B, C, D and E. All animals in groups A, B, C and D were treated with Azithromycin (10mg/kg b.wt), Metronidazole (50mg/Kg b.wt), Allium sativum (50mg/Kg b.wt) and Paromomycin (100mg/kg b.wt) respectively while Group-E was placed as a positive control group. The highest percent efficacy in reduction of OPG was shown by different drugs such as Paromomycine (91.77%) followed by Metronidazole (78.20%), Allium sativum (77.00%) while the lowest percent efficacy was shown by Azithromycin (59.29%). On the basis of hematological study, lower lymphocytes count was (48.39%) recorded in non-infected animals while higher (54.33%) count was recorded in infected animals. Similarly higher eosinophil count was (6.73%) recorded in infected group while lower (50 %) counts were recorded in non-
Summary
157
infected group. Hb level was higher in infected group than healthy animals. PCV level was higher (42.94%) in infected animals while low (34.62%) in healthy animals. Biochemical analysis of the serum showed, higher quantity of total protein, albumin, ALP, Sodium, Potassium, Chloride, Zinc, Copper, Urea and Creatinine was recorded in infected goats while lower quantity was observed in healthy goats. Availability: Items available for loan: UVAS Library [Call number: 2880-T] (1).
327.
Comparative Efficacy Of Mycotoxin Binders And Effects Of Aflatoxin B1 On Health Status Of Lactating Beetal Goats
by Haq Aman Ullah (2005-VA-180) | Prof. Dr. Aneela Zameer Durrani | Dr. Muhammad Ijaz | Dr. Aqeel Javeed.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: Aflatoxins are the secondary metabolites produced by moulds particularly by certain strains of Aspergillus flavus and Aspergillus parasiticus. The most toxic is the aflatoxin B1 (AFB1) and is considered as Hepatocarcinogenic. In present study aflatoxin B1 contamination status of different concentrate feeds (cotton seed cake, wanda, wheat bran and homemade concentrate mixture) of dairy goats was investigated in district Lahore of Pakistan. Twenty goat farms were randomly selected and 40 feed samples (two from each farm) were collected. The samples were analysed for the estimation of AFB1 using reverse phase High Performance Liquid Chromatography (HPLC) in Quality Operations Laboratory, UVAS, Lahore. AFB1 was detected in 33 feed samples out of 40 thus with a percent contamination rate of 83%. The quantity of aflatoxin B1 ranged from 0-225.736 ppb (μg/kg). The maximum level of AFB1 was detected in cotton seed cake (mean level 137.059 ± 22.293 ppb) and minimum level was detected in wheat bran (mean level 5.676 ± 1.047 ppb). Amongst the positive concentrate feed samples, 28 samples (85% of the positive) were having the concentration of AFB1 higher than the permissible level recommended by European Communities which is 5 ppb for concentrates. Thus the milk from goats consuming AFB1 contaminated concentrate feed can be a potential hazard for public health. This part of the study fulfills the first objective of the research project, which is to determine the aflatoxin B1 contamination status of concentrate feeds of dairy goats in District Lahore.
Beetal is the best dairy goats’ breed of Pakistan. In second part of the study, lactating beetal goats were used. In this experiment, effects of dietary AFB1 on health status of lactating beetal goats and its transfer from feed into milk as metabolite aflatoxin M1 (AFM1) were investigated. Thirty two lactating beetal goats of 3-4yr old, 6-8 wks. lactation period and weighing 40.91 ± 0.285 Kg, were selected from Small Ruminant Farm, Pattoki, University of veterinary and Animal
97
Sciences Lahore, and were randomly divided into four groups A, B, C and D, each having 8 animals. Group A was kept as negative control, while groups B, C and D were fed with aflatoxin B1 through naturally contaminated cotton seed cake. The cotton seed cake was having high level of AFB1 which was 150 μg/kg. After 7 days of adaptation period, each animal of groups B, C and D was individually fed with 30μg, 40μg and 50μg of aflatoxin B1 per day respectively through naturally contaminated cotton seed cake for a 10 days period., thus each animal of groups B, C, and D was fed with 200 g, 266.66 g, and 333.33 g of cotton seed cake per day respectively, to provide the required amount of AFB1 to each experimental animal. Milk samples were collected 24hr before the first AFB1 feeding and on 3rd, 7th and 10th day post aflatoxin B1 feeding. The samples were tested for AFM1 through HPLC, somatic cell count (SCC) and total viable count (TVC). Blood samples were analyzed for aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) levels before and after aflatoxin B1 administration. AFM1 was detected in all milk samples of the Groups B, C and D, with concentration beyond permissible level which is 0.05ppb (European communities).The amount of AFM1 excreted in milk was positively correlated with the amount of aflatoxin B1 ingested. The AFB1 was excreted in the milk as AFM1 at 1.35-1.59%. The mean SCC of groups A, B, C and D was 1.44×106 ± 1.75×105, 4.13×106 ± 4.09×105, 3.63×106 ± 4.60×105, 1.38×106 ± 1.56×105 cells/ml respectively. TVC obtained was 2.4×104 ± 1.9×103, 4.3×104 ± 1.6×103, 4.8×104 ± 3.3×103, 3.6×104 ± 3.3×103 cfu/ml of milk for group A, B, C and D respectively. The mean ALP levels were 62 ± 7, 223 ± 22, 147 ± 14, 175 ± 16 U/L for group A, B, C and D respectively. Mean levels of AST of group A, B, C and D were 79 ± 2.214, 110 ± 5.386, 104 ± 2.015, 126 ± 9.456 respectively. Mean levels of ALT of group A, B, C and D were 14 ± 0.326, 20 ± 1.118, 21 ± 1.106, 28 ± 1.250 U/L respectively. The ingestion of AFB1 even at low dose of 30μg in goats resulted in excretion
98
of AFM1 in milk beyond the permissible level. SCC, TVC, AST and ALT levels increased with ingestion of dietary AFB1. This part of the study fulfills the 2nd objective of the research project which is to investigate the effects of dietary aflatoxin B1 on health status of lactating beetal goats and its transfer from feed into milk as AFB1.
In the third part of the study, the efficacy of two different mycotoxin binders Toxfin® and Elitox® was determined, in terms of reduction in milk AFM1 excretion, improvement in milk quality and liver health. Groups A and B were kept negative and positive controls respectively. Experimental animals, cotton seed cake and number of groups remained the same as used in part 2 of the experiment. After initial 10 days of the experiment, each animal of groups B, C and D was daily fed with 266.66 g of cotton seed cake to provide 40 μg of AFB1 per day to each animal, for next 7 days. In the meantime, group C was fed with mycotoxin binder Toxfin® (Kemin industries, Inc. USA), at a dose rate of 3 g per Kg of cotton seed cake, while group D was fed with mycotoxin binder Elitox® (Impextraco Belgium ) at a dose rate of 1 g per Kg of cotton seed cake. Toxfin® and Elitox® both significantly decreased the excretion of AFM1 in goats’ milk, detected through high performance liquid chromatography (HPLC). Toxfin® and Elitox® reduced the excretion of AFM1 in milk by 56 % and 48 % respectively, thus the detoxifying ability of Toxfin® in feed was significantly higher than Elitox®. The SCC and TVC remained statistically unchanged after the administration of mycotoxin binders. The enzyme activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were not significantly affected by the mycotoxin binders. The alkaline phosphatase (ALP) level significantly increased in animals receiving either Toxfin® or Elitox®. The mycotoxin binders used in this study were not able to significantly improve milk quality and liver’s health in AFB1 treated goats, during the experimental period. This part of the study fulfills the 3rd objective of the research project, which was to determine the comparative efficacy of two different mycotoxin binders in lactating goats fed with mold contaminated diet. Availability: Items available for loan: UVAS Library [Call number: 2860-T] (1).
328.
Clinical echocardiography of the dog and cat
by Madron, Eric de.
Edition: 1st ed.Material type: ; Literary form:
not fiction
Publisher: USA: Elsevier; 2016Availability: Items available for loan: UVAS Library [Call number: 636.08961207543 Madron 32350 1st 2016 CMS] (1).
329.
Anesthesia and analgesia for veterinary technicians
by Thomas, John A | Lerche, Phillip.
Edition: 5th ed.Material type: ; Literary form:
not fiction
Publisher: China: Elsevier; 2017Availability: Items available for loan: UVAS Library [Call number: 636.089796 Thomas 32349 5th 2017 CMS] (1).
330.
Small animal surgical nursing : skills and concepts
by Busch, Sara J | Tear, Marianne.
Edition: 1st ed.Material type: ; Literary form:
not fiction
Publisher: Canada: Elsevier; 2017Availability: Items available for loan: UVAS Library [Call number: 636.08970231 Busch 32357 1st 2017 CMS] (1).
331.
Small animal clinical techniques / 2nd ed.
by Taylor, Susan Meric.
Edition: 2nd ed.Material type: ; Literary form:
not fiction
Publisher: China: Elsevier; 2016Availability: Items available for loan: UVAS Library [Call number: 636.089 Taylor 32353 2nd 2016 CMS] (1).
332.
Performing the small animal physical examination
by Englar, Ryane E.
Edition: 1st ed.Material type: ; Literary form:
not fiction
Publisher: Singapore: Wiley Blackwell; 2017Availability: Items available for loan: UVAS Library [Call number: 636.0896075 Englar 32351 1st 2017 CMS] (1).
333.
Reconstructive surgery and wound management of the dog and cat
by Kirpensteijn, J.
Edition: 1st ed.Material type: ; Literary form:
not fiction
Publisher: Spain: Manson Publishing; 2013Availability: Items available for loan: UVAS Library [Call number: 636.7089795 Kirpensteijn 32356 1st 2013 CMS] (1).
334.
Practical transfusion medicine for the small animal practitioner / 2nd ed.
by Sink, Carolyn A.
Edition: 2nd ed.Material type: ; Literary form:
not fiction
Publisher: Malaysia: Wiley Blackwell; 2017Availability: Items available for loan: UVAS Library [Call number: 636.089539 Sink 32352 2nd 2017 CMS] (1).
335.
Small Animal Surgery
by Fossum,Theresa Welch | Duprey, Laura Pardi | Huff, Theodore G.
Edition: 4th ed.Material type: ; Literary form:
not fiction
Publisher: Canada: Elsevier; 2013Availability: Items available for loan: UVAS Library [Call number: 636.0897 Fossum 32501 4th 2013 CMS] (1).
336.
Color Atlas of Diseases and Disorders of Cattle
by Blowey, Roger W | Weaver, A. David.
Edition: 2nd ed.Material type: ; Literary form:
not fiction
Publisher: China: Elsevier; 2003Availability: Items available for loan: Pattoki Library [Call number: 636.20896 Blowey 32506 2nd 2003 CMS] (1).
337.
Principles and Practice of Veterinary Technology
by Sirois, Margi.
Edition: 2nd ed. Material type: ; Literary form:
not fiction
Publisher: USA: Mosby; 2004Availability: No items available Checked out (1).
338.
Clinical Medicine of the Dog and Cat
by Schaer, Michael.
Edition: 3rd ed.Material type: ; Literary form:
not fiction
Publisher: India: Replika Press; 2016Availability: Items available for loan: UVAS Library [Call number: 636.70896 Schaer 32714 3rd 2016 CMS] (1).
339.
Dollar's Veterinary Surgery
by Oconnor,JJ.
Edition: 1st ed.Material type: ; Literary form:
not fiction
Publisher: India: CBS Publishing, 2005Availability: Items available for loan: Pattoki Library [Call number: 636.0897 Oconnor 50292 1st 2005 CMS] (3). Checked out (1).
340.
Veterinary Surgical Techniques
by KUMAR,Amresh.
Edition: 1st ed.Material type: ; Literary form:
not fiction
Publisher: India: VIKAS; 2015Availability: Items available for loan: Pattoki Library [Call number: 636.0897 Kumar 50310 1st 2015 CMS] (1).
341.
Adams & Stashak's Lameness in Horeses
by Baxter,Gary M.
Edition: 6th ed.Material type: ; Literary form:
not fiction
Publisher: UK: Wiley Blackwell; 2011Availability: Items available for loan: Pattoki Library [Call number: 636.1089758 Baxter 50415 6th 2011 CMS] (1).
342.
Avian medicine and surgery : self-assessment color review
by Forbes, Neil A.
Edition: 2nd ed.Material type: ; Literary form:
not fiction
Publisher: India: CRC Press; 2017Availability: Items available for loan: UVAS Library [Call number: 636.50896 Forbes 32935 2nd 2017 CMS] (1).
343.
Textbook of Large Animal Surgery
by Oehme, Frederick W.
Edition: 2nd ed.Material type: ; Literary form:
not fiction
Publisher: India: east west press private limited. 2012Availability: Items available for loan: Pattoki Library [Call number: 636.110897 Oehme 29044 2nd 2012 CMS] (1).
344.
The Merck Veterinary Manual / 10th ed
by Aiello, Susan E | Moses, Michael A.
Edition: USA:Material type: ; Format:
print
; Literary form:
not fiction
Publisher: Merck, 2010 Availability: Items available for loan: Pattoki Library [Call number: 636.089 Aiello 29582 10th 2010 CMS] (1).
345.
A Textbook of Preventive Veterinary Medicine 4th ed.
by Chakrabarti Amalendu.
Edition: 4th ed.Material type: ; Format:
print
; Literary form:
not fiction
Publisher: New Delhi: Kalyani Publishers; 2011Availability: Items available for loan: UVAS Library [Call number: 636.0896 Chakrabarti 26092 4th 2011 CMS] (1).
346.
Textbook of Preventive Veterinary Medicine 3rd ed.
by Chakrabarti Amalendu.
Edition: 3rd ed.Material type: ; Format:
print
; Literary form:
not fiction
Publisher: New Delhi: Kalyani Publishers; 2006Availability: Items available for loan: UVAS Library [Call number: 636.0896 Chakrabarti 3rd 2006 26057 CMS] (1).
