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1. Effects Of Storage And Processing Condition On The Quality Of Whola Milk Powder

by Muhammad Yaqoob Ellahi | Prof.Dr. Muhammad Abdullah | Dr. Muhammad Ayaz | Dr. Saima.

Material type: book Book; Format: print Publisher: 2009Dissertation note: Milk is a complex mixture of fat, proteins, carbohydrates, minerals, vitamins and other miscellaneous constituents dispersed in water. Milk production in flush season is much more than the requirement. Milk production and supply fluctuate through out the year and during winter it is surplus to its demand. Dairy is one of the expanding industrial sectors in Pakistan; about 17 units are engaged in the production of various dairy products. Surplus milk is available in winter and this is the normal practice of dairy industry in Pakistan that surplus milk is converted in to different types of milk powders. The objectives of the study were to evaluate the physico- chemical and keeping quality of whole milk powder commercially available in the market and comparative study of functional properties among different brands of whole milk powders, evaluate the storage stability of different brands of whole milk powder atl5°C, 25°C and 40°C temperature and study the effects of processing conditions indicator like Hydroxymethyl Furfural (HMF) during storage. Project was undertaken to study the effect of storage temperatures (15, 25 and 40°C) and processing conditions on physico chemical and sensory characteristics of whole milk powder. Statistical analysis of whole milk powder samples indicated during storage non significant changes in protein, ash, fat, lactose, acidity, burnt particles within the treatments as a function of storage was observed. Significant changes were recorded in free fatty acids, solubility index and per oxide value within the treatments and during storage period of 120 days. At 120 days of storage minimum increase in free fatty acids and peroxide value was noted in T3. Acidity, free fatty acids and peroxide value increased throughout the storage period of 120 days but at 15 °C the effect of storage on free fatty acids and peroxide value was comparatively less than 25 and 40 °C. Hydroxy methyl furfural was found significant within the treatments and effect of storage period and temperature was also significant. Hydroxy methyl furfural almost remained same at 15 °C, with the increase of temperature it increased significantly. Sensory evaluation was carried out by a panel of six trained judges it was noted that score for color, taste; smell and mouth feel decreased with the increase of temperature and storage period. The highest score for color, smell, taste and mouth feel was obtained by T3 during 120 days of storage. Chemical analysis of whole milk powder samples showed that whole milk powder samples stored at 40 °C were most affected in terms of physico chmeical and sensory quality. Overall results indicated that physico chemical quality and sensory properties of T3 was found best and it was comparatively less affected by storage temperatures. On the basis of this study it is recommended that for proper storage of whole milk powder 15 °C temperatures is suitable. Availability: Items available for loan: UVAS Library [Call number: 1060,T] (1).

2. Effect Of Various Levels Of Probiotics(Lactobacillus Acidophilus And Bifidobacterium Bifidum) On Physicochemical, Microbiological And Sensory Characteristics Of Ice Cream

by Aliya Javed | Dr. Muhammad Ayaz | Dr. Saima | Muhammad Nadeem.

Material type: book Book; Format: print Publisher: 2010Dissertation note: Ice-cream is a frozen mixture of combination of components, such as milk, sweeteners, stabilizers, emulsifiers and flavoring agents. Ice-creams are food products which show excellent potential for delivering probiotics to consumer. Probiotics are basically health promoting gut friendly bacteria. Minimum viable quantity of probiotics which is beneficial for human beings is 106 or 107 cfu/g. Benefits are strain specific and cannot be extrapolated. Keeping in view the importance of probiotics the present research study was planned to determine the influence of various levels of probiotics (Lactobacillus acidophilus and Bifidobacterium bifidum) on physicochemical and sensory characteristics of ice cream, assessing the viability of probiotics at different storage periods and to give innovative and value added product to commercial ice cream manufacturers. In order to carry out the study, Freeze dried cultures of two probiotic bacteria i.e., Lactobacilus acidophilus (DVS LA-5 Probio-Tec®) and Bifidobacterium bifidum (DVS BB-12® Probio-Tec®) were obtained from Chr. Hansen (Hørsholm Denmark). The probiotic cultures were stored at - 18? C in freezer. These two probiotic strains were inoculated alone and in combination in ice cream mix at three different inclusion levels. Hence there were total nine experimental treatments. A regular or control ice cream i.e., without any probiotic culture was also used along with treatments. Probiotic ice cream preparation and physical tests were performed at walls Ice Cream factory, Lahore, Pakistan. Chemical analysis were performed at department of Food and Nutrition laboratory, whereas, Microbiological tests were conducted at department of Microbiology, University of Veterinary and Animal Sciences, Lahore. Probiotic ice cream was manufactured in the pilot plant of the research and development department, Unilever Walls Ice Cream factory Lahore. Ice cream mix was prepared by following standard procedure. After mixing, homogenization and pasteurization of ice cream mix, it was cooled to 41°C and was divided into ten equal parts. Probiotics cultures alone and in combination, according to experimental design, were added in ice cream mix. Then it was fermented for two hours at 41°C. Then mix was aged at 4°C for a period of 2 hours followed by freezing and hardening. Ice cream samples were packed in one liter plastic tubs and were stored at - 18°C in freezer. Ice cream samples were analyzed after every fifteen days interval during storage period of three months. Physicochemical tests including overrun, viscosity, melting resistance, pH, acidity, fat, protein, total solids were performed by following the methods of AOAC (2000). As far as results of physicochemical tests are concerned, it was observed that both Lactobacillus acidophilus and Bifidobacterium bifidum are able to produce acid in ice cream. Acidity tended to increase as a result of increase in the number of bacteria. Highest pH was observed in control (R) sample, whereas, lowest pH was observed for samples having mixed culture. pH tended to decrease throughout storage. Lactobacillus acidophilus found more acid producing than Bifidobacterium bifidum. Ice cream samples containing high levels of probiotic bacteria showed decreased value of overrun. Whereas no effect was found in case of viscosity. Samples with increased level of bacteria showed significantly less melting resistance. Also, melting resistance tended to decrease significantly with increase in storage. Total solids and fat contents remained constant throughout storage and effect of treatments was statistically non significant. In case of protein contents, a positive correlation was observed .Increase in level of bacteria , increased the protein contents of ice cream, but it remained constant throughout the storage. Viable probiotic bacteria were enumerated by using the technique of spread plate method by using RCA media. In present study, cell count of viable bacteria, after fermentation, showed increase number of colonies. Samples inoculated with B3 were excellent in regard of exhibiting probiotic property followed by treatments B2 and A3. Treatments in combination i.e., C1, C2 and C3 showed results close to treatments having single cultur , which might be due to nutrients competency with each other. Furthermore, it was quite difficult to count colonies in mixed culture. Ice cream samples prepared from mixed culture were more acidic in taste and flavour and were least liked by sensory evaluators. Probiotic ice cream was subjected for sensory evaluation by five panel of judges. They rated ice cream by using nine point hedonic scale. Color of ice cream did not show any change throughout storage and was not affected by various levels of probiotics. No surface spots were found in any treatment at any storage period. Due to acid producing nature of probiotics, slight acidic changes were observed in thickness, flavour and taste of ice cream. However, overall acceptability for all probiotic ice cream was good. The data were analyzed according to analysis of variance technique under factorial arrangement. Significance of means was compared by using Duncan's multiple range test. Availability: Items available for loan: UVAS Library [Call number: 1205,T] (1).

3. Physico-Chemical And Sensory Characteristics Of Feta Cheese Made From Sheep Milk Blends.

by Muhammad Adeel | Dr. Imran Javed | Dr. Muhammad Nasir | Dr. Saima Inayat.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1639,T] (1).

4. The Effect Of Date Palm Fruit (Phoenix Dactylifera L.) Extract On Oxidative Stabilization Of Butter At Ambient

by Fazal Rehman | Dr. Muhammad Nadeem | Dr. Saima Inayat | Dr. Yasir Zahoor.

Material type: book Book; Format: print ; Literary form: drama Publisher: 2013Dissertation note: This investigation aimed to explore the antioxidant potential of date fruit extract using butter as an oxidation substrate on the basis of certain instrumental, orthodox analytical techniques and sensory characteristics. The objectives of this study were to characterize the date extract for polyphenolic compounds, free radical scavenging activity and inhibition of nitric oxide macromolecules and to study the effect of various concentrations of the date palm extract on oxidative stability and sensory characteristics. The butter was added 200, 400 and 600-ppm respectively date palm extract and compared with a control, stored at refrigeration and ambient temperature during winter months for 90-days at the interval of 30-days. Peroxide value, anisidine value, iodine value, conjugated dines, totox value, free fatty acids and sensory evaluation were performed in the fresh and three month stored butters. Sensory evaluation of supplemented butter added with various concentrations of date palm extract stored revealed the effect of treatments, storage period and their interaction to be significant. Taste, Color and overall acceptability score was non-significant up to 30-days of storage. After this sensory score started to deteriorate and went on decreasing the rest of storage period. The decline in taste score was not due to the addition of date palm extract rather it was due to the generation of peroxides and other oxidation products. Oxidation products and peroxides are found to be in direct relation with temperature; with rise in temperature the decline in sensory evaluations was more pronounced. This connection between taste score and peroxide value was highly correlated (R= 0.9982). The results of triplicate treatments and triplicate analysis (n=3x3; ±SD n=3x3) was analyzed by using one way analysis of variance techniques (ANOVA) to find out the effect of the date palm extract to the storage conditions. Differences among treatments were compared by Duncan Multiple Range Test. Supplementation of butters with date palm fruit extract did not revealed any significant effect on the inhibition of free fatty acids (P>0.05). The free fatty acid content of fresh and 90 days stored treatments and control were at par with each other. The peroxide value of all the treatments and control increased during the storage period but to varying degree. The addition of date palm extract revealed a significant effect on inhibition of peroxide value (P<0.05). The peroxide value of fresh supplemented, control and 90 days stored samples showed a significant difference. The difference in control and treatments were in order of To > T 1 > T2 > T3 in 90-days stored experimental samples and control. T3 revealed a maximum resistance towards the increase of peroxide value during storage period of 90-days over the control at refrigeration temperature. It is evident from the result that the addition of date palm extract significantly inhibited the auto-oxidation phenomenon in butter stored at room temperature, however the concentration of oxidation products were higher as compared to butter stored at refrigeration temperature. The iodine value decreased during the storage period in control experimental samples throughout the storage period of 90-days but to varying extents at refrigeration temperature. Control (T0) showed highest decrease in iodine value while T3 showed least change in iodine value during the storage period. The anisidine value of control and treatments stored at refrigeration temperature were in the order T0 > T1 > T2 > T3. Control revealed significant increase in anisidine value as compared to treatments. After 90 days of storage period the extent of secondary oxidation products in control and T3 was 22.24 and 8.35 at refrigeration temperature respectively. The classical rise of anisidine value was observed in all the treatments to varying degree, supplementation of natural antioxidant offered a great deal of resistance towards the rise of anisidine value. Supplementation of butter with date palm fruit extract revealed significant effect on the generation of oxidation products. Conjugated dienes for control and all the treatments increased throughout the storage time slowly and steadily. Analysis of variance revealed that the effect of treatments, storage period and their interaction was significant. Taste, Color and overall acceptability score was non-significant up to 30-days of storage. After this sensory score started to deteriorate and went on decreasing the rest of storage period. The results indicate that ethanolic date palm extract at 600-ppm concentration can be used as natural antioxidant source for the long term storage of butter at ambient temperature during winter months. The outcome of this study will help the food industry for preservation of fats and oils. Availability: Items available for loan: UVAS Library [Call number: 1800,T] (1).

5. Quality Enhancement Of Soy Milk And Soy Yoghurt Blender With Buffalo Milk

by Muhammad Asad Hameed | Dr. Saima Inayat | Dr. Imran Javed | Dr. Muhammad Nasir.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2014Dissertation note: Yoghurt is a fermented milk product, which is produced by the bacterial fermentation of milk. It is a rich source of calcium, protein and vitamin B-complex. Lactose-intolerant people can eat yoghurt without any harm as lactose is converted into lactic acid by the bacterial culture. Yoghurt is more nutritive then milk and possesses better digestibility. The benefit of yoghurt depends upon the presence of beneficial viable bacterial culture in adequate number. The bacterial cultures are used in the fermentation process to metabolize the lactose, secondly the proteolysis of protein for improving bioavailability and thirdly lactic acid bacteria for production of some B-complex vitamins and vitamin K. Yoghurt culture are responsible for the production of aromatic flavor compounds. In Pakistan the production of soy milk and soy yoghurt isnot common because ofconsumerunawareness for such kind of products. Therefore, this study was designed to develop soy milk and soy yoghurt by using different concentrations of buffalo milk. To produce soy yoghurt, soy milk and whole milk was pasteurized at 85°C for 30 minutes to kill the pathogenic microorganism. Thencooled at 42°C. This milk was used for the production of yoghurt.Thecommercial starter culture was used for the manufacturing of soy yoghurt. This culture was imported from Italy. During storage the prepared soy yoghurt was evaluated for its sensory qualities. A panel of judges evaluated the yoghurt samples for appearance, taste, color and overall acceptability on 9-point hedonic scale (9 = like extremely; 1 = dislike extremely). The sensory evaluation of the product at 0, 7, 14 and 21 day was carried out in the Department of Dairy Technology, University of Veterinary and Animal Sciences, Ravi Campus, Pattoki. There was significant effect of storage on sensory attributes of soy yoghurt. Highest score was awarded to T0. Soy yoghurts were evaluated for physico-chemical parameters (Fat, protein, total solid, acidity, pH and ash) during storage of 21 days with 7 days interval. Fat, pH and ash contents were continuously decreased while protein, total solid and acidity values show continuous increase in all treatments. Soy yoghurt was also evaluated for microbiological examination (total plate). Highest microbial count was observed in T0. Availability: Items available for loan: UVAS Library [Call number: 1842,T] (1).

6. Qualitative Studies Of Cheese Made By Using Bifidobacterium Bifidum & Lactobacillus Acidophilus

by Abid hussain | Dr. Saima inayat | Dr. Jalees | Dr. Muhammad ayaz.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2042,T] (1).

7. Incidence Of Spore Former Microbes In Pasteurized Milk Available In Lahore Disteict

by Muhammad Asim ikram | Dr. Imran javed | Dr. Saima inayt.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2054,T] (1).

8. Development And Quality Enhancement Of Cottage Cheese Made From Nili Ravi Buffalo Postpartum Milk (Colostrum)

by Maryam Batool (2010-Va-360) | Dr. Saima Inayat | Dr. Muhammad Ayaz | Dr. Saeed Ahmad.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: Colostrum is milk obtained from mammals secreted after parturition. It differs from normal milk as it contains numerous bioactive components which include growth factors, lacto-peroxidase, lacto-ferrin, Igs, lysozyme, nucleosides, vitamins, oligosaccharides and peptides, which are health promoters. Cottage cheese is an excellent source of cyanocobalamin, riboflavin and pyridoxine. Using colostrum for making cottage cheese can give opportunities to increase cottage cheese quality which may be more functional, healthier with high yield. Preservation of colostrum in the form of cottage cheese can be supportive for obtaining functional cheese with enhanced shelf life. Nili Ravi Buffalo postpartum milk was used for the production of Cottage cheese. While the control was prepared from Nili-Ravi buffalo normal milk. Milk was pasteurized at 63 °C for around 30 minutes in batch pasteurizer. Then the milk temperature was brought down at 37°C for inoculation with the mother cultures mentioned above at 2% level. Three replicates for each treatment was prepared. Incubation continued for 30 minutes at 42°C. Then 1.7 ml CaCl2 was added and after 10 minutes 1.7 ml of rennet solution was added in cheese milk and left for another 40 minutes for coagulation. Then the curds was cut into 1cm3 and healed for 10 minutes. The temperature is raised to 55°C for cooking for 30 minutes. Then whey was drained for 24 hrs. The curd was pressed and shaped into mold and then wrapped in aluminum foil and stored at 4°C for 7 days for further analysis. The physicochemical analysis such as pH, Acidity%, Fat %, TS %, Ash %, Chlorides %, Casein %, Protein% and Lactose% of Colostrum and Colostrum based Cottage cheese with respect to control was carried out by standard methods as described in AOAC. The data thus collected were analyzed through one way analysis of variance under Completely Summary 139 Randomized Design (CRD). Means were compared through Duncan’s Multiple Range (DMR) Test (SAS 9.1 Statistical Software). Physicochemical analysis of Colostrum showed that pH is in the increasing trend with the increase of age of milk and results showed that Colostrum pH values lower than milk values and were related to the high proteins content that is usually revealed in colostrum obtained 2-3 days after birth. While the trend of titratable acidity %, fat %, chlorides %, specific gravity, ash %, Total solids %, Total protein % and casein % is decreasing with an increase in the age (Days) of postpartum milk. Physicochemical analysis of Colostrum Cottage Cheese showed that the trend of pH of colostrum cottage cheese remained constant with the increase of age. The trend of lactose% and ash % is increasing with an increase in the age of postpartum milk. While the trend of titratable acidity %, fat %, chloride%, total solids %, total protein % and casein % of cottage cheese is decreasing with an increase in the age (days) of postpartum milk. Microbiological analysis of Cottage cheese was examined. Coliforms remained absent (<10 cfu/g) during whole storage time. Total Plate Count (TPC) was not significantly different throughout the storage period as it renders growth if there are hygiene and proper storage conditions available. Sensory evaluation was be carried out using criteria of appearance, taste, color, flavor and whole acceptability on a hedonic scale of nine points. T0, T3, T4 and T5 showed cheese like aroma, T1 showed yoghurt like aroma while T2 showed cheese like/ slight acidic aroma after 7 days of storage period. T0 and T5 showed whitish appearance, T1 showed light yellow appearance, T2 showed slight creamy like color after 7 days of storage period while T3 and T4 showed off white appearance after 7 days of storage period. T0, T4 and T5 showed light acidic flavor, T1 and T2 Summary 140 showed slight saltish / most bitterness in taste while T3 showed acidic taste after 7 days of storage period. T0 and T5showed granular texture with no creaminess, T1 showed soft yoghurt like texture, T2 showed meaty / hard texture while T3 and T4 showed harder toffee like texture after 7 days of storage period. Colostrum is the most proteinaceous food as compared to milk and improves the nutritional aspects as well as quality and yield of the products. So Cottage cheese made from Colostrum is more nutritious, functional with probiotic characters as well as enhanced physico-chemical properties with high yield. Availability: Items available for loan: UVAS Library [Call number: 2473-T] (1).

9. Quality Enhancement Of Buffalo Milk Yoghurt By Using Glutathione Treated Transglutaminase Enzyme As A Gelatin Replacer

by H. M. Arslan Amin (2009-VA-486) | Dr. Saima Inayat | Dr. Muhammad Junaid | Prof. Dr. Khalid Javed.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: Yoghurt is a fermented dairy product and intensively used in all over the world. It is very popular in sub-continental region due to its high consumption as compare to other regions of the world. Fermentation of milk is carried out by mixture of lactic acid bacteria. Synergistic effect of Streptococcus thermophillus and Lactobacillus delbrueckii ssp. bulgaricus is a key factor which determines the rate of fermentation process and quality of yoghurt. In traditionally made set yoghurt, protein gel is stabilized by weak non covalent interactions (hydrogen bonding, electrostatic interactions and hydrophobic bonds). Addition of Transglutaminase enzyme (TGase) can provide opportunities to increase covalent bonding and cohesiveness which ultimately enhances the gel consistency with reduction of syneresis. For the production of yogurt standardized buffalo milk of 5.5% fat and 9.5% SNF contents were used. Milk was pasteurized for 15 seconds at 72°C to kill the pathogenic bacteria and cooled to 42°C. After cooling the Glutathione treated Transglutaminase enzyme was added in the milk with different concentrations (1.25g/250ml, 2.50g/250ml, 3.75g/250ml and 5g/250ml) and cooled to 42°C. While the control sample was prepared without the addition of Glutathione treated Transglutaminase enzyme. After that each treatment was inoculated with standard yoghurt culture (Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus) at the rate of 2% at 42°C for 3-4 hours and each sample was stored at 4°C in a refrigerator for 28 days. The physico-chemical parameters such as pH, acidity %, fat %, protein % and syneresis % were observed weekly till 4 weeks. The quality comparison of prepared yoghurt was carried out by using Two Way Analysis of Variance (ANOVA) technique under Completely Randomized Design. Significant means were compared using Duncan’s Multiple Range (DMR) Test with the help of SAS 9.1. Results showed variations in pH, acidity %, fat%, protein %, snf% and syneresis % values due to the production of Yoghurt with Glutathione treated Transglutaminase in different percentages. pH in different treatments decreased from 4.71 – 4.61. Acidity % during storage period increased from 0.40 % to 0.43 %. Fat % also showed some variation during storage period decreased from 5.37% – 4.49 %. Protein % in different treatments increased from 4.10 % – 4.27 % and SNF % in different treatments increased from 9.81% – 10.00 % . Syneresis % in different treatments decreased from 5.17% - 3.48 %. Sensory evaluation was carried out by a panel of 06 semi trained people, using criteria of appearance, taste, color, flavor and whole acceptability on a 9 points hedonic scale. Color, taste, aroma, acidity, appearance and overall acceptability showed non-significant results as compared with controlled sample after 28 days. Results of the present study showed that yoghurt prepared with Glutathione treated Transglutaminase enzyme reduced the syneresis and enhanced the nutritional aspects of yoghurt. Availability: Items available for loan: UVAS Library [Call number: 2483-T] (1).

10. Enhancement Of Omega Fatty Acids In Margarine Through Chia (Salvia Hispanica L.) Oil

by Hafiza Anam Baig (2010-VA-355) | Dr. Muhammad Nadeem | Dr. Saima Inayat | Dr. Muhammad Hayat Jaspal.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: The higher incidents of deaths from cardiovascular diseases have led to the identification and modification of risk factors for atherogenesis; it is regarded as the number one reason of deaths in developed and underdeveloped countries. Chia contains about 40% edible oil, with fairly high content of unsaturated fatty acids, oil contains about 60% omega-3 fatty acids on weight basis. Margarine will be prepared from palm oil, palm kernel oil, butter and chia oil blends by varying the concentration from 5% to 20%. Margarine was prepared by blending butter, palm oil and Chia seed oil in various proportions. Market margarine (Blue Band) was used as a positive control. Butter, Palm oil and Chia Seed oil was characterized for free fatty acids, moisture content, saponification value, unsaponifiable matter, iodine value, refractive index. Solid fat index was determined at 0, 10, 20, 30 and 40˚C by the dilatometric method, melting point was determined by open capillary tube technique. Colour, free fatty acids, iodine value, peroxide value, anisidine value, conjugated dienes and trienes was determined. Fatty acids composition of margarines was determined by the transformation into fatty acid methyl esters. Margarine was stored at 6±1oC, for 2 months, iodine value, peroxide anisidine values, free fatty acids, conjugated dienes and trienes was determined. Sensory evaluation was performed by a panel of ten trained panel of judges, samples was evaluated for color, smell, taste and overall acceptability on a 9-point Hedonic scale. Peroxide value of 60 days old control and T4 (12% chia oil) were 7.06 and 12.10 (MeqO2/kg). After 60 days of storage period, peroxide value was in the order of T4> T3> T2> T1> control. Free fatty acids increased with the storage period in all the treatments. They are also increasing with the treatments having higher concentration of chia oil. At 60 day, control Summary 68 and T4 are 0.16 and 0.19% respectively. Anisidine value trend is considerably accelerating along the treatments as well as with the storage time. At 60 day, control and T4 is 24.42 and 44.78 respectively. Conjugated Dienes have the increasing trend throughout the treatments. They also have higher values from 0 to 60 days of time period. At 6o day, the control and T4 are 2.47 and 3.34 respectively. Conjugated Trienes of all the samples increased throughout the storage period of 60 days. At 60 day, the control and T4 are 2.60 and 3.28 respectively. Iodine value is in the decreasing trend throughout the storage period and the treatments. They are in the order of T0>T1>T2>T3>T4. Fat content of all the treatments and the control went on decreasing throughout the storage period of 60 days. At 60 day, control and T4 were 68.61 and 90.91 respectively. The order of the fat content in all the treatments were T0>T1>T2>T3>T4. Moisture content of all the treatments and the control went on decreasing throughout the storage period of 60 days. At 0 day, Moisture content of all the treatments were virtually the same. At 60 day, control and T4 were 27.52 and 7.64 respectively. Salt content of all the treatments at the end of the storage period were increased. At 60 day, control and T4 were 4.18 and 1.42 respectively. The taste score of all the treatments along the treatments having different concentrations of unsaturated fatty acids and also along with the storage period of time is decreased. The taste score of the control and T4 were 8.06 and 6.90 respectively. The order of the taste score is in the descending order of T0>T1>T2>T3>T4. Results of this evidenced that concentration of omega fatty acids can be enhanced in margarine through chia oil with acceptable sensory characteristics. Major changes in fatty acid composition were observed around C18:2 and C18:3 (omega 6 and 3). Concentration of trans fatty acids in control, T1, T2, T3 and T4 was 24.75%, 6.67%, 5.49%, 4.71% and 3.19%. Availability: Items available for loan: UVAS Library [Call number: 2578-T] (1).



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