1.
Taxonomical Study Of Ticks Of Genus Rhipicephalus And Their Relation To The Incidence Of Haemoparasites And Comparative Efficacy Of Different Acaricides On Ticks In Sheep And Goats In Kaghan Valley
by Imtiaz Khan, M | Dr. Mubasher Saeed Mian | Dr. M. Afzal | Dr. Muhammad Sarwar Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1993Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0344,T] (1).
2.
Serodiagnosis Of Ovine Hydatidosis
by Javaid, M | Dr. Asif Rabbani | Dr. Mubasher Saeed Mian | Dr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1993Dissertation note: A study was under taken to find out the incidence of hydatidosis and to evaluate the efficacy of indirect haemagglutination (IHA) test for the confirmation of natural hydatidosis in sheep slaughtered at Lahore municipal abattoir.
Blood samples from 200 sheep (50 each from hydatidosis affected and free sheep on the basis of postmortem findings and 100 blood samples Elected randomly without considering postmortem finding). The serum from each sample was separated, properly labelled and stored at -20°C.
For the preparation of antigen, crude cyst fluid was aspirated aseptically from hydatid cysts. Blood from healthy sheep was collected in 3.8% sodium citrate solution and red blood cells were separated by centrifugation. A 2.5% red cell suspension was prepared in Phosphate Buffered Saline (PBS). The sheep erythrocytes were sensitized by Hydatid fluid antigen. Optimal dilution of antigen 1:16 was used in Phosphate Buffered Saline (p11 6.4) for sensitizing the sheep erythrocytes. All the sera were inactivated at 56°C for half an hour and serial two f1d serum dilutions were prepared by micropipettes in microtitre U plates and sensitized erythrocytes were added to the plates and incubated at room temperature in a humid chamber for 3 hours. A titre of 1:32 and above was considered as positive. In positive reactions, the cells agglutinated like a carpet at the bottom of the wells where as in negative cases the cells settled as a compact mass in the centre of the wells. By the indirect haemagglutination test Ninety-two percent sheep were found positive for hydatidosis. (Table-4.2). Out of 50 serum samples (Group A2), 46 (92%) were confirmed positive on postmortem while 3 out of 50 (6%) hydatid free samples (Group A3), gave false positive results with IHA test.
It was concluded that indirect haemagglutination test is an accurate, reliable and sensitive test (92%) for the diagnosis of hydatidosis in sheep.
The blood cell counts (TLC, DLC) and blood chemistry (Total protein, A/G ratio) of the samples under investigation were also carried out. From the results it was evident that the hydatid cysts did not affect the blood values of the host significantly. However, only 28% of hydatid positive animals showed eosinophilia ranging from 7 to 23% which was non pathognomonic.
It was thus inferred that blood cell counts and blood c1vmistry of the hydatid cyst patients was of no diagnostic value.
Availability: Items available for loan: UVAS Library [Call number: 0359,T] (1).
3.
A Study On The Chemotherapy And Taxonomy Of Mange Mites In Ovine And Its Effect On Different Blood Parameters
by Rafique Rana, M | Dr. Muhammad Afzal | Dr. Mubasher Saeed Mian | Prof. Dr. SAghir | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1993Dissertation note: A study was carried out to find out the taxonomy, chemotherapy of mange mites in sheep and its effect on different blood parameters. A total of 30 flocks comprising of fifteen hundred sheep of different age and sexes were examined in the city and villages around Lahore. Out of above mentioned animals 200 suspected sheep were thoroughly examined and 30 (15%) were found positive for mange mites infestation. Species of three genera namely Sarcoptes, Psoroptes and Chorioptes were found infesting these animals.
The average number of mites recovered from the species of each genera were recorded as 851, 825 and 815 for Psoroptes, Sarcoptes and Chorioptes respectively. For the identification of different species of mange mites microscopic examination was carried out and mites were first seen under low power and then high power of magnification. The slides were mounted with mites and then examined to study their morphological characteristics.
The three genera identified were Sarcoptes, Psoroptes and Chorioptes in sheep.
Chemotherapeutic trials were carried out in suspected sheep by using two concentrations of neguvon solution i.e. 0.10% and 0.15% at ten days intervals. For this purpose sheep were divided into three groups. Group A was treated with 0.10% solution of neguvon, group B with 0.15% solution of neguvon and group C was kept as untreated control group. The data obtained on reduction of mites due to acaricide application were subjected to statistical analysis by applying completely randomized design and results were recorded. It was noticed that 0.15% neguvon was highly effective against mange mites infestation when it was replied twice after ten days interval.
The effect of mange mites on different blood parameters was also recorded. For this purpose sheep were divided into three groups. Group I comprised of clinically infested sheep, group II subclinically infested sheep and group C included healthy sheep which was kept as control for the comparison. The effect of mange mites on different blood parameters of two infested groups of sheep i.e. I and II was recorded before and after two applications of 0.10% and 0.15% neguvon solution at ten days interval and results were compared with group C for comparison.
The findings of this study indicated that the infestation of mange mites is a quite common problem in sheep population of this area. The sheep suffering from mange mites exhibit poor feed intake, severe irritation, scratching, bleeding, loss of wool, low body growth, reduced production and damaged skin. Such infested sheep are also susceptible to various viral and bacterial diseases which makes sheep production uneconomical and put extra burden on the sheep breeders.
Keeping all this in view suitable and prompt measures should be taken for early diagnosis and treatment of the infested animals. This will result not only for the control of the disease, but also help to improve the economical condition of the owners. It will also increase the gross national product of Pakistan.
Availability: Items available for loan: UVAS Library [Call number: 0386,T] (1).
4.
A Study On The Prevalence & Taxonomy Of Biting & Sucking Lice Infestation In Sheep With Their Effect On Different Blood Parameters
by Jamal Abdul Nasir | Dr. Muhammad Afzal | Dr. Mubasher Saeed Mian | Dr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1995Dissertation note: The study was planned to determine the prevalence and taxonomy of biting and sucking lice infestation in sheep with their effect on various blood parameters. For this purpose specimens were collected from Outdoor Hospital College of Veterinary Sciences, Lahore and various private clinics in the city.
The collected specimens were preserved, stained and ide.ntified after preparing permanent mounts under the sterioscopic microscope in the laboratory. Two species of lice i.e. Damalinia (biting lice) and Liiioqnathus pedalis (sucking lice) were identified having a percentage of 10.50 and 1.0 respectively. The infestation recorded was highest during spring and autumn and lowest during the summer.
The taxonomy of the identified species were carried out by using morphological characters (Chandler, 1961 and Soulsby, 1982).
Haernatological examinations were made to compare the blood picture of healthy and infested sheep. For this purpose 20 blood samples each from healthy and infested sheep were examined. Five ml of blood was collected from the jugular vien of the sheep, with the help of disposable syringes, in a test tube having few drops of 0.1 % EDTA.
The blood parameters studied were: Haemoglobin level, Total Erythrocyte Count, Total Leukocyte Count, Packed Cell volume and differential leukocytic count. The results of haematological examination showed a significant decrease in haemoglobin, total erythrocyte count and packed cell volume, whereas a significant rise in leukocytic count and D.L.C. of infested sheep as compared to healthy sheep was observed.
The results of the present study will help in planning better control measures in order to minimize lice infestation and also aid in the eradication of various lice born diseases in sheep.
Availability: Items available for loan: UVAS Library [Call number: 0407,T] (1).
5.
Study Of Gastro-Intestinal Helminths And Taxonomy Of Species Of The Genus Paramphistomum In Camels
by Saeed A. Khan | Dr. Muhammad Afzal | Dr. Mubasher Saeed Mian | Dr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1994Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0411,T] (1).
6.
Comparative Efficacy Of Anthelmintics Against Gastro Intestinal Parasites Of Buffalo Calves And Their Effects On Blood Parameters
by Tariq Humayun | Dr. Mubasher Saeed Mian | Dr. Haji Ahmad Hashmi | Dr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1995Dissertation note: A total of 90 (60%) out of 150 fecal samples from buffalo calves of different age groups were found positive for gastrointestinal parasites. Coprological examination revealed Toxocara vitulorurn (42.22%) as single infection and 31.11% as mixed infection. Strongy.Zoides papillosus as 21.11% as single infection arid 20% mixed infection. Nematodirus spp. 1.11% as single. Haemonchus sp., 2.22% as single and 1.11% mixed infection. Honiezia sp. 3% as single and Eimeria spp., 21% as single and 13.33% as mixed infection. Intensity of infection of gastro-intestinal parasites in buffalo calves was determined as 41.1% of calves upto 500 eggs per gm of faeces, in 22.22% calves from 501 to 1000 epg, in 16.66% calves from 1001 to 1500 epg and in 20% calves greater than 1500 epg of faeces.
Comparative percentages of means of EPG counts of infected control subgroups of groups A,B and C showed increasing trend of EPG counts by day 14. However subgroup A3, showed 'I3.l% reduction, subgroup B3 showed 61.15% reduction and C) showed 72.29% reduction in epg counts on day 3 after medication with oxfendazole. Similarly on day 7 of medication, the reduction percentage were, 95.52, 89.93 and 96.38 in respective subgroups. Albendazole in subgroups A4, 84 and C4 showed reduction on day 3, by 67.08, 64.89 and 78.57 and 95.24 respectively. On day 14, both the anthelmintics showed 100% etticacy No significant change was noted in RBC, UBC counts Hb gm/mi and PCV%, however, Eosinophils and lymphocyte counts depicted siynificant increase in the infected subgroups.
Availability: Items available for loan: UVAS Library [Call number: 0439,T] (1).
7.
A Study Of Gastro Intestinal Helminthiasis And The Effects Of Natural Infection On Various Blood Parameters In The Buffaloes
by Ishtiaq Ahmad | Dr. Asif Rabbani | Dr. Mubasher Saeed Mian | Dr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1995Dissertation note: A total of 76 buffaloes brought to the Lahore Metropolitan Slaughter house were studied for helminthiasis. Out of which 50 (65.79%) were found positive.
The helminthiasis positive guts were examined for the presence of helminths and the following species were identified Haemonchus contortus (11.84%), Haemonchus placei (10.53%), Ostertagia ostertagi (7.89%), Trichostrongylus axei (3.95%), Mecistocirrus digitatus (2.63%), Trichostrongylus colubriformis (7.89%), Cooperia oncophora (9.21%), Cooperia punctata (5.26%), Cooperia pectinata (6.58%) , Nematodirus helvetianus (2.63%), Bunostomum phiebotomum (2.63%), Strongyloides papillosus (2.63%), Oesophagostornum radiatum (3.95%), Trichuris globulosa (1.32%), Paramphistomum cervi (3.95%), Paramphistomum microbothrium (2.63%) and Monezia benedeni (2.63%)
The effect of helminthiasis on blood parameters like, total erythrocytic count, total leukocytic count, packed cell volume, erythrocytic sedimentation rate, haemoglobin concentration was investigated and the values recorded were 5.840±0.110x106 Cimm, 5.447±0.074x103 Cumm, 32,858±0.230%, 38.004±0.217 mm/hr. and 10.368±0.096 gm/lOOml respectively. The differential leucocytic count values for neutrophils, lymphocytes, eosinophils, monocytes and basophils were
43.16±0.531%, 43.82±0.718%, 6.40±0.187%, 5.620±0.241% and 1.040±0.124% respectively.
Similarly the helminth free (control> animals studied for their total erythrocytic count, total leukocytic count, packed cell volume, erythrocytic sedimentation rate, haemoglobin concentration, gave their averages as 7.382±0.068x106 Cmm, 6.321±0.084x103 Cimm, 38.927±0.286%, 28.213±0.148 mm/hr. and 11.892±0.286 gm/lOOml respectively. The differential leukocytic count values for neutrophils, lymphocytes, eosinophils, monocytes and basophils were
29.15±0.365%, 56.0±0.513%, 8.12410.28%, 5. 852410 .264% and 0.90±0.191% respectively.
The average values for helminth infected and helminth free animals were analysed statistically. The infected animals had a significantly decreased total erythrocytic count, total leukocytic count, packed cell volume, haemoglobin concentration, lymphocytes and eosinophils with increased erythrocytic sedimentation rate and neutrophils, while no significant difference was seen in monocytes and basophils.
Availability: Items available for loan: UVAS Library [Call number: 0445,T] (1).
8.
A Study On Gastro Intestinal Nematodes Of Camels Slaughtered At Metropolitan Corporation Abattoir Lahore With Taxonomy Of The Isolated Species
by Abbas Ali, Syed | Dr. Asif Rabbani | Dr. Mubasher Saeed Mian | Dr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1993Dissertation note: The present study was carried out to determine the incidence of gastro-intestinal nematodes of camels, with the taxonomy of the isolated species. Sixty guts were examined rendomly at the rate of 15 guts per month from August to November, 1993. The adult parasites were collected, fixed and preserved. The whole mounts were prepared. The faecal samples were examined and the EPG was estimated using McMaster egg counting technique. The taxonomy of the isolated species was determined by examing the whole mounts, according to the morphological characters given in the keys. The five species of nematodes representing different genera were isloated and identifed as Haenionchus ion gistipes, Izlaemonchus contortus, C'ainelostrongy!us mentulatus, Neniatodirus spathiger and Trichuris giobulosa.
The overall incidence recorded from camels during the present study was 65 percent. The morphological characters were studied by preparing permanent mounts and were described.
The findings of the present study will help in planning programme for the control of parasitic disease, especially caused by the above mentioned species of nematodes of camels in Pakistan, which will contribute to the development of animal industry by providing much effective coverage for the devastating effects of the parasites and will ultimately enchance the production of milk, meat and hides.
Availability: Items available for loan: UVAS Library [Call number: 0463,T] (1).
