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1. Taxonomical Study Of Ticks Of Genus Rhipicephalus And Their Relation To The Incidence Of Haemoparasites And Comparative Efficacy Of Different Acaricides On Ticks In Sheep And Goats In Kaghan Valley

by Imtiaz Khan, M | Dr. Mubasher Saeed Mian | Dr. M. Afzal | Dr. Muhammad Sarwar Khan | Faculty of Veterinary Sciences.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 1993Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0344,T] (1).

2. Serodiagnosis Of Ovine Hydatidosis

by Javaid, M | Dr. Asif Rabbani | Dr. Mubasher Saeed Mian | Dr. Muhammad | Faculty of Veterinary Sciences.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 1993Dissertation note: A study was under taken to find out the incidence of hydatidosis and to evaluate the efficacy of indirect haemagglutination (IHA) test for the confirmation of natural hydatidosis in sheep slaughtered at Lahore municipal abattoir. Blood samples from 200 sheep (50 each from hydatidosis affected and free sheep on the basis of postmortem findings and 100 blood samples Elected randomly without considering postmortem finding). The serum from each sample was separated, properly labelled and stored at -20°C. For the preparation of antigen, crude cyst fluid was aspirated aseptically from hydatid cysts. Blood from healthy sheep was collected in 3.8% sodium citrate solution and red blood cells were separated by centrifugation. A 2.5% red cell suspension was prepared in Phosphate Buffered Saline (PBS). The sheep erythrocytes were sensitized by Hydatid fluid antigen. Optimal dilution of antigen 1:16 was used in Phosphate Buffered Saline (p11 6.4) for sensitizing the sheep erythrocytes. All the sera were inactivated at 56°C for half an hour and serial two f1d serum dilutions were prepared by micropipettes in microtitre U plates and sensitized erythrocytes were added to the plates and incubated at room temperature in a humid chamber for 3 hours. A titre of 1:32 and above was considered as positive. In positive reactions, the cells agglutinated like a carpet at the bottom of the wells where as in negative cases the cells settled as a compact mass in the centre of the wells. By the indirect haemagglutination test Ninety-two percent sheep were found positive for hydatidosis. (Table-4.2). Out of 50 serum samples (Group A2), 46 (92%) were confirmed positive on postmortem while 3 out of 50 (6%) hydatid free samples (Group A3), gave false positive results with IHA test. It was concluded that indirect haemagglutination test is an accurate, reliable and sensitive test (92%) for the diagnosis of hydatidosis in sheep. The blood cell counts (TLC, DLC) and blood chemistry (Total protein, A/G ratio) of the samples under investigation were also carried out. From the results it was evident that the hydatid cysts did not affect the blood values of the host significantly. However, only 28% of hydatid positive animals showed eosinophilia ranging from 7 to 23% which was non pathognomonic. It was thus inferred that blood cell counts and blood c1vmistry of the hydatid cyst patients was of no diagnostic value. Availability: Items available for loan: UVAS Library [Call number: 0359,T] (1).

3. A Comparative Study Of Lungworms In Sheep & Goats & The Effect Of Mixed Namatode Infection On Certain Blood Parameters

by Irshad Ahmed Sherazi | Dr. Sh,. Asif Rabbani | Dr. Muhammad | Dr. Muhammad Afzal | Faculty of Veterinary Sciences.

Material type: book Book; Format: print Publisher: 1994Dissertation note: The present study was conducted to find out the incidence of lungworm infestation, identify the different species and to observe the effect of mixed nematode infections on certain blood parameters in sheep and goats. The study was conducted at Parasitology Laboratory, College of Vety. Sciences Lahore. For this purpose sheep & goats brought to Lahore abattoirs for slaughtering were closely examined before and after slaughter and animals suspected for lungworms were selected for collection of blood and tissue samples. 200 samples of lungs and blood 100 each from sheep and goats were collected for parasitological and haematolog ical examination, respectively. The results revealed that incidence of lungworms infestation in sheep and goats was 31 and 11 %, respectively. It was observed that Dictyocaulus filaria, Protostrongylus rufescence and Muellerius capillaris infection in lung of sheep was in the order of 22, 8, and 1 % respectively, whereas, the incidence of lungworms infestation in lungs of goats caused by the same species was 8, 3 and 0 %, respectively. The highest incidence of lungworms infection observed was that of Dictyocaulus filaria in both the sheep and goats, while Muellerius capillaris was found only in lungs of one sheep and infection by this species could not be detected in goats. The findings of this study showed that average red blood cell count, heamoglobin level and packed cell volume were reduced due to nematode infection in sheep and goats. The results have also indicated that nematode infection in sheep and goats enhanced erythrocyte sedimentation rate. From the results of the present study, it may be concluded that incidence of lungworm infestation in sheep and goats is on the higher side which warrants that suitable treatment and control measures should be adopted to safeguard our valuable livestock from this serious infection. It is also evident from the results that nematode infection adversly affects the components of blood in sheep and goats which may lead to anaemia and loss of growth and production. Availability: Items available for loan: UVAS Library [Call number: 0371,T] (1).

4. Experimental Induction Of Coccidiosis In Broiler Chicks With Eimeria Tenella And Comparative Efficacy Of Different

by Noora Jan Issot | Dr. Hajid Ahmad Hashmi | Dr. Muhammad | Dr. Muhammad Afzal | Faculty of Veterinary Sciences.

Material type: book Book; Format: print Publisher: 1994Dissertation note: The study was designed to look into the immunogenic properties of Eimeria tenella. Two hundred and twenty five day old broiler chickens were reared under controlled conditions. They were equally divided into five groups i.e. A, B, C, D, and E. Group 'A' was kept as uninfected control. Group 'B' acted as infected control, which was infected with 50,000 sporulated oocysts at day 15, 25 and 35 of age. Group 'C' acted as infected and treated, which received sporulated oocysts on day 15, 25 and 35 alongwith 5 days treatment with salinomycine (60 ppm in finished feed) after 3 days of each primary and challenge dose. To group 'D' 1000 live sporulated oocysts were administered orally at day 3 and 10. Similarly, 'E' group received formalin treated 1000 sporulated oocysts orally at day 3 and 10 of age of chickens. Subsequently the members of group D and E received challenge infections of 50,000 Eimeria tenella sporulated oocysts at day 15, 25 and 35. Immunogenicity was measured by the passage of oocysts per gm of faeces and by comparative weight gains amongst different groups. It was noticed that no coccidiosis could be established in groups A, C and E and the OPG counts remained nil throughout the period of experimnt. Group B showed high counts and group 'D' showed moderate ounts but towards the completion of experiment the OPG counts gradually reduced to 8400 and 6100 respectively, in both group. Weight gains recorded were 473, 393, 316 and 310 gin. greater in A, E, D, and C respectively, as compared with group B. Group E depicted 1OC% protection and remained second to the uninfected control group A in respect of weight gains. Group 'D', although developed infection but due to acquired immunity became resistant to challenge infections and was placed 3rd in weight gain. Inspite of complete protection in group C due to Salinomycine treatment, the weight gains remained lower than group D and E. Availability: Items available for loan: UVAS Library [Call number: 0381,T] (1).

5. A Study On The Chemotherapy And Taxonomy Of Mange Mites In Ovine And Its Effect On Different Blood Parameters

by Rafique Rana, M | Dr. Muhammad Afzal | Dr. Mubasher Saeed Mian | Prof. Dr. SAghir | Faculty of Veterinary Sciences.

Material type: book Book; Format: print Publisher: 1993Dissertation note: A study was carried out to find out the taxonomy, chemotherapy of mange mites in sheep and its effect on different blood parameters. A total of 30 flocks comprising of fifteen hundred sheep of different age and sexes were examined in the city and villages around Lahore. Out of above mentioned animals 200 suspected sheep were thoroughly examined and 30 (15%) were found positive for mange mites infestation. Species of three genera namely Sarcoptes, Psoroptes and Chorioptes were found infesting these animals. The average number of mites recovered from the species of each genera were recorded as 851, 825 and 815 for Psoroptes, Sarcoptes and Chorioptes respectively. For the identification of different species of mange mites microscopic examination was carried out and mites were first seen under low power and then high power of magnification. The slides were mounted with mites and then examined to study their morphological characteristics. The three genera identified were Sarcoptes, Psoroptes and Chorioptes in sheep. Chemotherapeutic trials were carried out in suspected sheep by using two concentrations of neguvon solution i.e. 0.10% and 0.15% at ten days intervals. For this purpose sheep were divided into three groups. Group A was treated with 0.10% solution of neguvon, group B with 0.15% solution of neguvon and group C was kept as untreated control group. The data obtained on reduction of mites due to acaricide application were subjected to statistical analysis by applying completely randomized design and results were recorded. It was noticed that 0.15% neguvon was highly effective against mange mites infestation when it was replied twice after ten days interval. The effect of mange mites on different blood parameters was also recorded. For this purpose sheep were divided into three groups. Group I comprised of clinically infested sheep, group II subclinically infested sheep and group C included healthy sheep which was kept as control for the comparison. The effect of mange mites on different blood parameters of two infested groups of sheep i.e. I and II was recorded before and after two applications of 0.10% and 0.15% neguvon solution at ten days interval and results were compared with group C for comparison. The findings of this study indicated that the infestation of mange mites is a quite common problem in sheep population of this area. The sheep suffering from mange mites exhibit poor feed intake, severe irritation, scratching, bleeding, loss of wool, low body growth, reduced production and damaged skin. Such infested sheep are also susceptible to various viral and bacterial diseases which makes sheep production uneconomical and put extra burden on the sheep breeders. Keeping all this in view suitable and prompt measures should be taken for early diagnosis and treatment of the infested animals. This will result not only for the control of the disease, but also help to improve the economical condition of the owners. It will also increase the gross national product of Pakistan. Availability: Items available for loan: UVAS Library [Call number: 0386,T] (1).

6. A Study On The Taxonomy Of Sheep Cestodes And The Efficacy Of Albendazole And Miclosamide Against Natural Infections

by Ashraf, M | Dr. Asif Rabbani | Dr. Mubashar Saeed Mian | Dr. Muhammad | Faculty of Veterinary Sciences.

Material type: book Book; Format: print Publisher: 1994Dissertation note: Pakistan has a large population of live stock, which plays an important role in the economy of the country. Parasitism adversally effects the growth and production of livestock. Great economic losses have been attributed to the sheep population in our country due to the parasitic infections including cestodes. A study of cestodal infections with taxonomy of the prevalent cestoctes species affecting sheep at different age groups in and around Lahore was conducted. The taxonomical study was conducted in the month of August, September and October, 1993. For this purpose 200 guts (one hurtdered each from below six month and above six month of sheep) were collected from Lahore Abattoir. Parasites were identitied under the microscope by preparing permanent mounts. The over all incidence of cestodes infection in sheep was 65.5%. The species of various genera of cestodes observed during the present study were: Moniezia pansa (64%), Moniezia benedeni (60%), Avitellina cjj[iur1ctta (49.50%), Avitellina lahorea (40%), Avitellina sudanea (30%), Stilesia vittata (30%), Stilesia globiounctata (50%) and Thvsanosoma actinioides (6%). Monthwise prevalence of cestodes infection was also carried out and it was observed that the highest infection rate was (76%) during the month of August and (66.25%) during the month of September. While the lowest incidence was recorded as (57.14%) during the month of october, 1993. The taxonomy of the prevalent cestode species was carried out. The effect of age of the animals on the intensity of cestodal infection was also studied. There was a significant difference in the prevalence of cestode infections among the two groups. The present work was also aimed to verify the efficacy of two commonly available anthelmintics namely albendazole (valbazen) and niclosarnide (mansonil), in naturally infected stceep with Gastrointestinal cestocJes. Fifty sheep positive for cestods infections were randomly divided into two groups A and B, each group comprising of 25 animals. Group. A was given albendazole (valbazen) at a dose rate of 2.5 mg/kg body weight orally while the group B was administered niclosamide (mansonil) at a dose rate of 100 mg/kg body weight orally. The efficacy of both the drugs was evaluated on the bases of reduction of segments and number of eggs per gram of faeces after medication. The egg counts were made on zero, 3rd and 21st day. Both the drugs revealed a progressive decrease in the faccal egg/segment counts. The efficacy of albenclazole on 3rd and 21st day was 96.02 and 99.23% repectively while the efficacy of niclosamide (mansonil) was 100% on 3rd and 21st day. Availability: Items available for loan: UVAS Library [Call number: 0387,T] (1).

7. A Study On The Prevalence & Taxonomy Of Biting & Sucking Lice Infestation In Sheep With Their Effect On Different Blood Parameters

by Jamal Abdul Nasir | Dr. Muhammad Afzal | Dr. Mubasher Saeed Mian | Dr. Muhammad | Faculty of Veterinary Sciences.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 1995Dissertation note: The study was planned to determine the prevalence and taxonomy of biting and sucking lice infestation in sheep with their effect on various blood parameters. For this purpose specimens were collected from Outdoor Hospital College of Veterinary Sciences, Lahore and various private clinics in the city. The collected specimens were preserved, stained and ide.ntified after preparing permanent mounts under the sterioscopic microscope in the laboratory. Two species of lice i.e. Damalinia (biting lice) and Liiioqnathus pedalis (sucking lice) were identified having a percentage of 10.50 and 1.0 respectively. The infestation recorded was highest during spring and autumn and lowest during the summer. The taxonomy of the identified species were carried out by using morphological characters (Chandler, 1961 and Soulsby, 1982). Haernatological examinations were made to compare the blood picture of healthy and infested sheep. For this purpose 20 blood samples each from healthy and infested sheep were examined. Five ml of blood was collected from the jugular vien of the sheep, with the help of disposable syringes, in a test tube having few drops of 0.1 % EDTA. The blood parameters studied were: Haemoglobin level, Total Erythrocyte Count, Total Leukocyte Count, Packed Cell volume and differential leukocytic count. The results of haematological examination showed a significant decrease in haemoglobin, total erythrocyte count and packed cell volume, whereas a significant rise in leukocytic count and D.L.C. of infested sheep as compared to healthy sheep was observed. The results of the present study will help in planning better control measures in order to minimize lice infestation and also aid in the eradication of various lice born diseases in sheep. Availability: Items available for loan: UVAS Library [Call number: 0407,T] (1).

8. A Comparative Study Of Helminth And Haemoparasites Of Domestic And Wild Pigeons

by Asma Hussain | Dr. Mubashir Saeed Mian | Dr. Asif Rabbani | Dr. Muhammad | Faculty of Veterinary Sciences.

Material type: book Book; Format: print Publisher: 1995Dissertation note: There is an increasing interest in pigeons and other game and ornamental birds. These birds are generally kept either free roaming or confined in outdoor pens and hence are vulnerable to various parasitic infectious, which greatly effects the productivity of these birds. The meat production of the pigeons can be improved by controlling disease problems particularly helrninths and blood protozoan infections, so that in near future they may contribute towards narrowing down the animal protein supply gap by substituting poultry meat with squabs. The present work was planned for the comparative study of helminths and heemoparasites of domestic and wild pigeons. For this purpose 300 each of guts and blood smears were studied. The overall incidence of gastrointestinal helminths and blood protozoans was 77.33% and 31.99% respectively, while 36% of birds had mixed infection. The incidence of gastro-intestinal helminths and blood protozoa in wild pigeons was 89.33 and 20.66 percent respectively and in domestic pigeons it was 65.33% and 11.33% respectively. Whereas mixed infections were 22% and 14% respectively. The following species of helmitiths and blood protozoa were recorded and identified. 1. Raillietina tetragona 2. Raillietiiia cesticillus 3. Choanolaenia infundibulum 4. Ascaridia colurnbae and 5. Cap illaria obgnata The two species of blood protozoa recovered were: 1. Aegypanella pullorum and 2. Haeiçotuscumbae Among the helrninths recorded, cestodes were found predominating as compared with nernatodes in both wild and domestic pigeons. Raillietina cesticillus was the most common cestode species in both Wild and domestic pigeons i.e. 51 and 33 percent respectively while Asci colurnbae predominated the nernatode species i.e. 18 and 15 percent respectively. The Incidence was higher in wild pigeons. Aegyptianella pullorum was more common blood protozoan i.e. 22 and 11 percent respectively in wild and domestic pigeons. The incidence of Haemoproteus clumbae was 9 and 11 percent respectively. Availability: Items available for loan: UVAS Library [Call number: 0410,T] (1).

9. Study Of Gastro-Intestinal Helminths And Taxonomy Of Species Of The Genus Paramphistomum In Camels

by Saeed A. Khan | Dr. Muhammad Afzal | Dr. Mubasher Saeed Mian | Dr. Muhammad | Faculty of Veterinary Sciences.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 1994Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0411,T] (1).

10. A Study Of Gastro Intestinal Parasitism And Haematological Disturbances Associated With Single Or Multiple Infection In Sheep

by Hafeez, M | Dr. Asif Rabbani | Dr. Mubashar Saeed Mian | Dr. Muhammad | Faculty of Veterinary Sciences.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 1996Dissertation note: Nature has blessed Pakistan with a large population of livestock which play an important role in the economy of the country. Parasitism adversely effects the growth and production of livestock. Great economic losses have been attributed to the sheep population in our country due to parasitic infections including gastro-intestinal parasitism. A study of gastro-intestinal parasitism with isolation and identification of the parasite species affecting sheep at different age groups in and around Lahore was conducted in the months of July, August, September and October, 1994. For this purpose 200 G.I. tracts of sheep (One hundred each from below six months and above six months of age) were collected from Lahore Metropolitan Corporation, Abattoir. The overall incidence of gastro-itestinal parasitism in sheep was found to be 68.5%. Age group wise incidence was found to be 71% and 66% in sheep below and above six months of age respectively. Classwise overall incidence was found to be Sporozoan (51%), Trematodes (21%), Cestodes (62%) and Nematodes (68%). Age group wise incidence of Sporozoan, Trematodes, Cestodes and Nematodes was found to be 60% and 42%, 12% and 30%, 71% and 53%, 73% and 63% in sheep below and above six months of age. Month-wise prevalence of gastro-intestinal parasitism was also carried out which was higher in the month of August and lowest in the month of October. Nineteen different species of Parasites were recorded which are detailed as under: 1. Eimeria arloingi 2. Eimeria ninakohlvakimovae 3. Eimerla parva 4. Elmerla intricata 5. Eimeria faurei 6. Paramphistomum cervi 7. Cotylophoron cotvlophorum 8. Moniezia. expansa 9. Moniezia benedeni 10. Avitellina centripunctata 11. Haemonchus contortus 12. Oesophagostomum colunThianum 13. Oesophagostonim venulosum 14. Trichuri 15. Ostertagia circumcincta 16. Ostertagia ostertagi 17. Chabertia ovina 18. Trichostrongvlus colubriformis 19. Nematodirus spathiger The isolation and identification of various collected parasites was carried out. The relationship between the age of sheep and the occurrence of gestro-Intestinal parasitism was also studied. There was a Non Significant difference in the prevalence of gastro-intestinal parasites among the two groups. The present project was also aimed to observe the effect of single or multiple parasitism on certain haematological parameters like, estimation of haemoglobin contents, total erythrocytic count, total leukocytic count, erythrocyte sedimentation Rate, packed cell volume and differential leukocytic count. These heamatological studies were carried out on control (parasitic free) and parasitised sheep of below and above six months of age. The findings of haematological studies showed that there was decrease in haemoglobin contents. total erythrocytic count, packed cell volume, total leukocytic count and increase in erythrocyte sedimentation rate in both sheep below and above six months of age under the effect of single or multiple gastrointestinal parasitism. The values of differential leukocytic count were variable in both age groups. Most of the haematological disturbances on various blood parameters in both age group of sheep were proved to be statistically significant. From the results of the presents study, it has been concluded that the incidence of gastro-intestinal parasitism in sheep below and above six months of age is on the higher side which warrants that certain essential preventive measures must be adopted to safeguard our valuable livestockfrom these serious parasitic infestations. It is also evident from the results that gastro-intestinal parasitism adversely affects the blood components in sheep which may lead to anaemia, loss of growth and loss of production. Availability: Items available for loan: UVAS Library [Call number: 0433,T] (1).

11. Comparative Efficacy Of Anthelmintics Against Gastro Intestinal Parasites Of Buffalo Calves And Their Effects On Blood Parameters

by Tariq Humayun | Dr. Mubasher Saeed Mian | Dr. Haji Ahmad Hashmi | Dr. Muhammad | Faculty of Veterinary Sciences.

Material type: book Book; Format: print Publisher: 1995Dissertation note: A total of 90 (60%) out of 150 fecal samples from buffalo calves of different age groups were found positive for gastrointestinal parasites. Coprological examination revealed Toxocara vitulorurn (42.22%) as single infection and 31.11% as mixed infection. Strongy.Zoides papillosus as 21.11% as single infection arid 20% mixed infection. Nematodirus spp. 1.11% as single. Haemonchus sp., 2.22% as single and 1.11% mixed infection. Honiezia sp. 3% as single and Eimeria spp., 21% as single and 13.33% as mixed infection. Intensity of infection of gastro-intestinal parasites in buffalo calves was determined as 41.1% of calves upto 500 eggs per gm of faeces, in 22.22% calves from 501 to 1000 epg, in 16.66% calves from 1001 to 1500 epg and in 20% calves greater than 1500 epg of faeces. Comparative percentages of means of EPG counts of infected control subgroups of groups A,B and C showed increasing trend of EPG counts by day 14. However subgroup A3, showed 'I3.l% reduction, subgroup B3 showed 61.15% reduction and C) showed 72.29% reduction in epg counts on day 3 after medication with oxfendazole. Similarly on day 7 of medication, the reduction percentage were, 95.52, 89.93 and 96.38 in respective subgroups. Albendazole in subgroups A4, 84 and C4 showed reduction on day 3, by 67.08, 64.89 and 78.57 and 95.24 respectively. On day 14, both the anthelmintics showed 100% etticacy No significant change was noted in RBC, UBC counts Hb gm/mi and PCV%, however, Eosinophils and lymphocyte counts depicted siynificant increase in the infected subgroups. Availability: Items available for loan: UVAS Library [Call number: 0439,T] (1).

12. A Study Of Gastro Intestinal Helminthiasis And The Effects Of Natural Infection On Various Blood Parameters In The Buffaloes

by Ishtiaq Ahmad | Dr. Asif Rabbani | Dr. Mubasher Saeed Mian | Dr. Muhammad | Faculty of Veterinary Sciences.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 1995Dissertation note: A total of 76 buffaloes brought to the Lahore Metropolitan Slaughter house were studied for helminthiasis. Out of which 50 (65.79%) were found positive. The helminthiasis positive guts were examined for the presence of helminths and the following species were identified Haemonchus contortus (11.84%), Haemonchus placei (10.53%), Ostertagia ostertagi (7.89%), Trichostrongylus axei (3.95%), Mecistocirrus digitatus (2.63%), Trichostrongylus colubriformis (7.89%), Cooperia oncophora (9.21%), Cooperia punctata (5.26%), Cooperia pectinata (6.58%) , Nematodirus helvetianus (2.63%), Bunostomum phiebotomum (2.63%), Strongyloides papillosus (2.63%), Oesophagostornum radiatum (3.95%), Trichuris globulosa (1.32%), Paramphistomum cervi (3.95%), Paramphistomum microbothrium (2.63%) and Monezia benedeni (2.63%) The effect of helminthiasis on blood parameters like, total erythrocytic count, total leukocytic count, packed cell volume, erythrocytic sedimentation rate, haemoglobin concentration was investigated and the values recorded were 5.840±0.110x106 Cimm, 5.447±0.074x103 Cumm, 32,858±0.230%, 38.004±0.217 mm/hr. and 10.368±0.096 gm/lOOml respectively. The differential leucocytic count values for neutrophils, lymphocytes, eosinophils, monocytes and basophils were 43.16±0.531%, 43.82±0.718%, 6.40±0.187%, 5.620±0.241% and 1.040±0.124% respectively. Similarly the helminth free (control> animals studied for their total erythrocytic count, total leukocytic count, packed cell volume, erythrocytic sedimentation rate, haemoglobin concentration, gave their averages as 7.382±0.068x106 Cmm, 6.321±0.084x103 Cimm, 38.927±0.286%, 28.213±0.148 mm/hr. and 11.892±0.286 gm/lOOml respectively. The differential leukocytic count values for neutrophils, lymphocytes, eosinophils, monocytes and basophils were 29.15±0.365%, 56.0±0.513%, 8.12410.28%, 5. 852410 .264% and 0.90±0.191% respectively. The average values for helminth infected and helminth free animals were analysed statistically. The infected animals had a significantly decreased total erythrocytic count, total leukocytic count, packed cell volume, haemoglobin concentration, lymphocytes and eosinophils with increased erythrocytic sedimentation rate and neutrophils, while no significant difference was seen in monocytes and basophils. Availability: Items available for loan: UVAS Library [Call number: 0445,T] (1).

13. Effects Of Theileriasis On Various Blood Parameters And Total Serum Protein In Exotic And Cross Bred Cattle Present In And Around Lahore

by Arshad Mahmood, Ch | Dr. Muhammad Afzal | Dr. M. Sarwar | Dr. Muhasher Saeed Mian | Faculty of Veterinary Sciences.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 1994Dissertation note: A study was conducted on Theileriasis in rfleilel.ia species, as well as haematological values were conducted by using 28 crossbred and 22 exotic cattles. Theileria annulata was the only species identified in the erythrocytes of infected cattle. Clinically, signs of panting, temperature ranging from 103.8 to 105.8°F, swelling of superficial lymph nodes, salivation and decrease in milk production were observed in infected animals. There were significant differences in haematological values in infected cattle as compared with non-infected ones. There was decrease in total erythrocytic count, haemoglobin concentration, packed cell volume, Total leukocytic count, serum protein and neutrophils. Whereas lymphocytes showed an increase in the infected cattle. Regarding the comparison of crossbred and exotic cattle, there was statistically significant difference in both the means of total erythrocyte count, PCV, Haemoglobin, Serum Protein, lymphocytes and neutrophils. But no significant difference was seen between means of eosinophils, Basophils and monocytes of the exotics as well as the crossbred cattle. Availability: Items available for loan: UVAS Library [Call number: 0450,T] (1).

14. The Effects Of Induced Coccidiosis On Growth And Blood Parameters In Commercial Quails (Coturnix Coturnix

by Ashraf, M | Dr. Asif Rabbani | Dr. Mubashar Saeed | Dr. Muhammad | Faculty of Veterinary Sciences.

Material type: book Book; Format: print Publisher: 1994Dissertation note: The study was designed to see the effects of experimentally induced coccidiosis op growth and blood parameters in commercial quails (coturnix coturnix japonica). For this purpose 200 day old quail chicks were obtained from a local hatchery and raised under standard, controlled coccidia free conditions. A commercial coccidiostat free feed was provided. At the age of 21 days, birds were divided into 2 groups A and B each comprising 100 birds. Birds of group 'A' were kept as non infected control and birds of group 'B' were infected with I ml of coccidial inoculum having 50,000 sporulated oocysts given directly into crop. After the infection, the OPG of the both groups was recorded. Weekly weight gain/bird of both A and B group was recorded. Meanwhile the morbidity, mortality, clinical finding and post-mortem finding were also observed. At the end of experimental period of 42 day, 20 birds from each group were slaughtered and blood sample were collected for haematology. The weight of birds in group 'B' was significantly lower than that of group 'A' birds. The morbidity and mortality rate in group B were 100% and 38% respectively. Availability: Items available for loan: UVAS Library [Call number: 0451,T] (1).

15. A Study On Gastro Intestinal Nematodes Of Camels Slaughtered At Metropolitan Corporation Abattoir Lahore With Taxonomy Of The Isolated Species

by Abbas Ali, Syed | Dr. Asif Rabbani | Dr. Mubasher Saeed Mian | Dr. Muhammad | Faculty of Veterinary Sciences.

Material type: book Book; Format: print Publisher: 1993Dissertation note: The present study was carried out to determine the incidence of gastro-intestinal nematodes of camels, with the taxonomy of the isolated species. Sixty guts were examined rendomly at the rate of 15 guts per month from August to November, 1993. The adult parasites were collected, fixed and preserved. The whole mounts were prepared. The faecal samples were examined and the EPG was estimated using McMaster egg counting technique. The taxonomy of the isolated species was determined by examing the whole mounts, according to the morphological characters given in the keys. The five species of nematodes representing different genera were isloated and identifed as Haenionchus ion gistipes, Izlaemonchus contortus, C'ainelostrongy!us mentulatus, Neniatodirus spathiger and Trichuris giobulosa. The overall incidence recorded from camels during the present study was 65 percent. The morphological characters were studied by preparing permanent mounts and were described. The findings of the present study will help in planning programme for the control of parasitic disease, especially caused by the above mentioned species of nematodes of camels in Pakistan, which will contribute to the development of animal industry by providing much effective coverage for the devastating effects of the parasites and will ultimately enchance the production of milk, meat and hides. Availability: Items available for loan: UVAS Library [Call number: 0463,T] (1).

16. A Study On The Epidemiological Aspects Of Fascioliasis In Buffaloes In Lahore District

by Rabbia Sahar | Dr. Asif Rabbani | Dr. Haji Ahmed Hashmi | Dr. Muhammad | Faculty of Veterinary Sciences.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 1996Dissertation note: The study was designed to find the prevalence and intensity of liver fluke infection in buffaloes in Lahore District. The study period was from April 1995 to July 1995. The data was based on: Data collected from four Veterinary Hospitals in Lahore District namely, Herbenspura, R.A. Bazar, Shamkey Bhattian and Rukh Chandra indicated that during the study period from 1 April, 1995 to 31 July, 1995, a total of 2184 buffaloes were treated for different diseases in these hospitals. Based on history, clinical symptoms and response to treatment 229 (10.48%) buffaloes were positive for fascioliasis. Information collected from Municipal Corporation, Lahore abattoirs regarding economical losses revealed that one hundred eight million rupees were lost annually due to damage and condemnation of liver fluke infected livers. Two hundred faecal samples collected directly from the rectum of buffaloes from various localities at Lahore were examined microscopically by fresh smear and sedimentation methods, which revealed that 75 (37.5%) buffaloes were suffering from fascioliasis. In order to determine the intensity of Fascioliasis in infected animals, quantitative faecal examination was done (and it ranged from 32.6 to 45.6 EPG indicating that the disease is likely to be pathogenic). Overall liver fluke infection was recorded by examining forty livers along with their bile ducts collected from slaughtered buffaloes and it was found that 16 (40%) had liver fluke infection. Identification of live flukes from the infected flukes revealed that 8 (50%) had mixed infection of Fasciola hepatica and Fasciola gigantica, 5 (31.25%) had only Fasciola gigantica infection while 3 (18.75%) had only Fasciola hepatica infection. It was concluded from the above studies that Fascioliasis in buffaloes in Lahore district is quite prevalent, and it needs to adopt appropriate measures to control the infection. Availability: Items available for loan: UVAS Library [Call number: 0485,T] (1).

17. Taxonomuy And Prevalence Of Eimeria Species Infecting Young Buffalo And Cattle Calves And Their Effect On Various

by Afzal, M | Dr. Muhammad Afzal | Dr. Haji Ahmad Hashmi | Dr. Shakil | Faculty of Veterinary Sciences.

Material type: book Book; Format: print ; Nature of contents: biography; Literary form: Publisher: 1996Dissertation note: This study was contemplated to evaluate some aspects of prevalent species including taxonomy of Eimeria infection& in young cattle and buffalo calves. In addition the effects of Eimeria infections on various haematological parameters i.e., Haemoglobin, PCV, RBC count, ESR and Differential leucocyte count were also studied. For this purpose the animals (Buffalo and Cattle calves) were divided into two groups. Firstly those having diarrhoea and secondly those which were apparently healthy. The buffalo and cattle calves were divided into four groups (A, B, C and D) on the basis of the age in days under the maximum age of 120 days. A group ranged from 1-30 days, B ranged 31- 60 days, C ranged 61-90 and D ranged 91-120 days. The haematological observations were recorded from both the groups formulated upon the basis of age as A, B, C and D in both cattle and buffalo calves and findings compared with the control groups A, B, C and D having the same age range. Results revealed that out of 816 animals (442 cattle and 374 buffalo calves), 167 (84 cattle and 83 buffalo calves) were found to be positive (20.46%) for Eimeria species infection. Whereas 62 out of 250 (24.80%) suffering from diarrhoea and 22 out of 192 (11%) apparently healthy cattle calves showed coccidial infections. For buffaloes 63 out of 220 cases (29%) were positive from animals showing diarrhoea and 20 out of 154 (13%) were found positive for coccidiosis from apparently healthy group. Four species of Eimeria namely, Eimeria zuernii, Elmerip bovis, Eimeria cylindrica and Eimeria ellipsoidalis were found in the above mentioned ca1ves E. zuernhi was the most prevalent species found (48%) followed by . bovis (27%), Eimeria cvlindrica (16%) and Eimeria ellipsoidalis (10%) respectively. OPG count carried out ranged from 2500-70,000 oocyst per gram of faeces. Haematological observations showed anaemia, which was a feature of the diarrhoeic calves and not found in apparently healthy animals although some of them had been harbouring Eimeria infection. The PCV decreased proportionately with haemoglobin whereas erythrocyte count also showed decrease in positive cases. On the other hand ESR of the above referred calves increased. There was neutrophilia in the infected animals versus control animals and lymphocytes decreased in infected animals. There was no significant change in eosinophil and monocyte counts. Availability: Items available for loan: UVAS Library [Call number: 0511,T] (1).

18. Taxonomical Studies Of Prevalent Species Of Eimeria In Broiler Chicks And Comparison Of Immucox (Imported Vaccine) Versus Locally Prepared Vaccine Against Coccidiosi

by Sodaghar Ali Shaker | Dr. Muhammad Afzal | Dr. Haji Ahmad Hashmi | Dr. Shakil | Faculty of Veterinary Sciences.

Material type: book Book; Format: print Publisher: 1997Dissertation note: The study was designed to identify different species of the genus Eimeria in broiler chicks occuring in poultry farms situated in and around Lahore. For this purpose one thousand faecal samples were collected from different broiler farms out of which 145 (14.5%) were positive for coccidiosis. Two hundred broiler carcases, suspected to he suffering from coccidiosis were obtained from different diagnostic Laboratories at Lahore. One hundred and ninety out of 200, (95%) were found positive for coccidiosis. Identification of the species of the genus Eimeria was done and seven species were identified i.e, E tenella 119 out of 190 (62.63%), J. brunetti 27 (14.21%), E. necatrix 13 (6.84%), . acervulina 19 (10%), . maxima 4 (2.10%), . mitis 3 (1.58%) and . praecpx 5 (2.63%). The second aim of the present study was to compare the immunogenic properties of locally prepared (atlenuated) and imported (live) vaccine (Immucox). For this purpose one hundred and fifty five day old broiler chicks were reared under controlled conditions. They were equally divided into five groups i.e. A, B, C, D and E. Group A was kept as uninfected control. Group B acted as infected control. Group B, C, D and E were infected with 50,000 sporulated Oocysts at day 15, 25, 35 and 45 of age. Group C acted as infected and treated control alongwith 5 days treatment with amprolium, after three days of each primary and challenge doses. Group D received 1000 sporulated formalin treated oocysts orally, at the age of day 3 and day 10. The members of group E received orally imported vaccine (Immucox) at the age of day 5. Immunogenicity was measured by the passage of oocysts per gram of faeces (OPG) and by comparative weight gains amongst different groups. It was noticed that no coccidiosis could be established in groups A, C, D and E. The OPG counts remained nil in groups A and C throughout the experimental period. Groups D and E showed moderate counts and Group B showed high counts of OPG. The OPG counts gradually decreased to 35,000, 1200 and 1150 in groups B, D and E, respectively at the end of the experiment. Average weight gain records showed 430, 150, 332 and 270 gms greater in A, C, D and E groups, respectively at the end of the experiment, as compared to group B. FCR of group A, B, C, D and E was 2.82, 3.48, 3.11, 2.76 and 2.93, respectively at the end of the experiment. Group D was the best and group B was the worst. Group D depicted full protection and remained second to the uninfected control group (A) in respect of weight gains. Group E also showed complete protection and remained third to the uninfected control group A in respect of weight gains. Inspite of complete protection in group C due to amprolium treatment, the weight gains remained lower than groups D and E. There was no development of exogenous species of Elmeria due to immunization with immucox. Availability: Items available for loan: UVAS Library [Call number: 0533,T] (1).

19. Studies On The Incidence Of Gastro Intestinal Helminths And Their Economic Impact On Production Potential Of Sheep

by Zia ur Rehman, Qazi | Dr. Asif Rabbani | Dr. Muhammad | Dr. Muhammad Afzal | Faculty of Veterinary Sciences.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 1998Dissertation note: A study of gastro intestinal helminth parasites of sheep and goats of District Peshawar has been conducted, covering a period of 4 months (May to August, 1998). During this study incidence of GIT. helminths and their impact on production potential (weight gain) of sheep and goats was studied. Random collection of samples were made from Peshawar slaughter house and a total of 40 guts each in sheep and goats were examined and following species of helminths were recovered and identified. Oesophagostomum columbianum, 0. venulosum, Trichuris parvespiculum, Haemonchus contortus, Chabertia ovina, Bunostornum trigonocephalum, Nematodirus spathiger, Moniezia benedeni, Moniezia expansa, Trichuris lani, T. globulosa, Cysticercus tenuicollis. Only two species of cestodes and nine species of nematodes were recorded during the study. However no trematode specie was recorded. Amongst the nematodes Trichiurisparuespiculum infection was recorded to be the highest in sheep and goats with an incidence of 64.70% and 73.33% respectively. The incidence of Haemonchus contortus was 24.19 and 53.33% in sheep and goats respectively. The worm burden ranged from 1-470 in sheep and 3-141 in goats respectively. Out of total gastro intestinal tracts examined (40 each in sheep and goats), 73.33% and 60.29% were found positive for mixed infection in sheep and goats respectively. In order to assess the effect of GIT helminths on live weight gain and its measurement in terms of economics of the above mentioned animals, 0 Hasthnagri sheep and 30 beetal goats were divided into two groups (control vs. treated by Nilzan Plus of ICI). The average daily weight gain in sheep group was 83.78 gms while in goats it. was 85.26 gms per animal. On comparison with treated group, the increase weight gain was 3.03 kg and 2.56 kg in sheep and goats respectively at the end of the experiment. Calculating the increase on live weight basis Rs.234.5 and 158 per animal were earned more in sheep and goats respectively. After deducting the cost of anthelmintic treatment the net gain Rs.222.5 and Rs.146 per animal in sheep and goats. During this study a progressive decrease in faecal egg counts with increasing age was observed both in sheep and goats. Statistical analysis by using un-paired "t" test confirmed that there was significant differences in weight gain and faecal egg counts, between control and treated groups of sheep and goats. Availability: Items available for loan: UVAS Library [Call number: 0563,T] (1).

20. The Prevalence & Control Of Natural Infestations Of Ectoparasites & Their Effect On Blood Parameters On Indigenous Birds

by Irfan Mueed, M | Dr.Kamran Ashraf | Dr. Muhammad Afzall | Faculty of Veterinary Sciences.

Material type: book Book; Format: print Publisher: 2000Dissertation note: A study was conducted to record the incidence and identification of various species of ectoparasites (ticks, lice, mites), and to find out the efficacy of ivermectin against ectoparasites in indigenous poultry. A total of 2000 indigenous birds were examined, during June to August, 1999 and out of these 1035 were found infested with ectoparasites, showing an overall infestation rate of 51.75%. The infestation rate of ticks, lice, mites and mixed infestation (ticks and lice) was recorded as 6, 16.5, 2.25 and 27 percent, respectively. The species of ectoparasites identified were: Argas persicus (fowl tick), Menopon gallinae, Menacanthus stramineus, Liveurus caponis, Goniodes gigas (lice) and Cnemidoco1)tes mutans (Scaly Leg Mite). In order to assess the efficacy of ivermectin (Ivomec) one hundred and fifty naturally infested birds with different species of ectoparasites were selected for treatment trials and divided into three groups i.e. A, Band C, each comprising of 50 birds. The birds of group A and B were the test groups and group C served as control group. Fifty birds of group A given ivermectin intramuscularly at a dose rate of 200Ji/kg (0.2 mg/kg) body weight and 50 birds were treated with ivermectin orally at the same dose rate of 10 mg/5 gallons of drinking water as for group A. The efficacy of the drug was determined on the basis of negative skin scrapings and absence of other ectoparasites (lice, ticks) from the body of the test group birds as recorded on day zero, 14th and 28 of post-medication of ivermectin. The cure percentage recorded in group A and B on day 28 of the experiment was 94 and, 90 percent in birds treated by intramuscular injection and oral treatment of the drug, resp~ctively. Haematological examinations were made to compare the blood picture of Infested birds treated with ivermectin and birds infested with ectoparasites and kept as untreated control. For this purpose blood samples from treated and untreated infested birds were examined. Blood parameters studied were: Total erythrocytic count (TEC), Packed cell volume (PCV), haemoglobin (Hb) Crythrocyte sedimentation rate (ESR), Differential leukocytic count DLC). The results of haemotological examinations showed a significant decrease in erythrocytic count, haejmoglobin, packed cell volume and rise in erythrocyte sedimentation rate and increase in differential leukocytic count of treated birds as compared to infested birds. Availability: Items available for loan: UVAS Library [Call number: 0620,T] (1).

21. Estimation Of Blood Haemoglobin, Serum Iron And Magnesium Level In Clinical Cases Of Ovine Haemonchosis

by Haneef ur Rehman | Dr . Asif Rabbani | Dr . Mubashar Saeed Mian | Dr . Muhammad | Faculty of Veterinary Sciences.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 1985Dissertation note: Haemonchosis is a serious helminthic infection of sheep and goats resulting in the mortality due to excessive blood loss and causes great economic losses. The objective of the present study was to determine the effect of natural infection of Haemonchus contortus in sheep upon blood haemo- globin, serum iron and magnesium level so that it could aid in accurate diagnosis and effective therapeutic measures. Infection was confirmed through faecal examination. Eggs per gram of faeces were calculated by McMaster egg counting technique. To see the effect of severity of infection on these blood components, blood samples of positive cases were taken from jugular vein. Blood haemoglobin, serum iron and magnesium were estimated by using standard diagnostic kits with spectrophotometer. A decreased level of haemoglobin and serum iron was directly proportional to the severity of infection. However, no alteration in serum magnesium level was observed in infected and non-infected animals. Availability: Items available for loan: UVAS Library [Call number: 0701,T] (1).

22. Occurrence And Importance Of Enteric Protozoan Infections On Different Goat Farms In District Swat

by Farooq Shah | Dr. Kamran Ashraf | Dr. Muhammad | Prof. Dr. Haji Ahmed hashmi | Faculty of Veterinary Sciences.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2005Dissertation note: A study was conducted to ascertain the prevalence and importance of enteric protozoa in goat population. A total of 225 faecal samples were collected from fifteen different goat farms in district Swat. These samples were collected from three age groups i.e Group I (day 1 to 6 months), Group II (7 - 12 months) and Group III (over one year). These samples were processed by Direct Smear method, Sheather's Flotation and Zinc Sulphate Flotation technique for the detection of Giardia, Cryptosporidium and Eimeria cysts and oocysts. Overall prevalence of Eimeria 37.77%, Giardia 12.44% and Cryptosporidium 6.22% was recorded. Age related prevalence showed highest the number of Eimeria 53.33%, Giardia 18.66% and Cryptosporidium 8% in Group I which comprised animals of I day to 6 month. Among 225 faecal samples 73, were collected from diarrhoeic and 152 from non diarrhoeic animals. The infection rates were higher in diarrhoeic animals, Eimeria 45.22%, Giardia 21.9% and Cryptosporidium 10.99% than non diarrhoeic animal. 34.6% faecal samples were found positive for other parasites such as Haemonchus 10.22%, Trichostrongylus 8%, Trichuris 20%, Strongyloids 6.6%. A large number of faecal samples (58.66%) were found positive for parasitic infections. Results of this study indicated widespread prevalence of' parasitic diseases in goat population and especially Eimeria and Giardia are the predominant protozoan parasites Availability: Items available for loan: UVAS Library [Call number: 0897,T] (1).

23. Seroprevalence Of Ftoxoplasma Gondii Infection In Sheep And Goats

by Sher Hayat Khan | Prf.Dr. Azhar Maqbool | Dr. Muhammad | Prof. Dr. Haji Ahmad Hashmi | Faculty of Veterinary Sciences.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2005Dissertation note: Keeping in view the zoonotic importance of Toxoplasmosis the present study was carried out to determine the seroprevalence of Toxoplasma gondii infection in sheep and goats using commercially available kit "Toxopalasmosis Latex" based on the principal of Latex agglutination. . For this purpose 100 blood samples (50 sheep & 50 goats) were collected randomly from slaughter house of Lahore and local sheep & goat forms, the serum was separated and analyzed for anti Toxoplasma antibodies. The animals were divided in three groups, Group I included animals below one year, Group II 1-4 years and Group III included animals aged 4 years and above. The overall 8% and 6% seroprevalence were recorded in sheep and goats respectively. Age titer relationship in sheep showed that Group III had (12.5%), Group 11(7.6 %) and Group I had no positive cases in sheep examined. In goats Group III had (10 %), Group II (5.7%) and no seropositive cases in Group I could be recorded. These results indicate that the infection was higher in older animal than young ones, Breed wise seroprevalence indicated that, Kchhi sheep 14.28%, Desi (non descript) 8.33% and no seropositive case in Lohi and Thali breeds was recorded. In goats Teddy breed had 11.11%, Desi (non descript) 5.55% while no seropositive case in Beetal goats could be recorded. From the data analyzed it is concluded that although the seroprevalence of T gondii infection in sheep and goats is relatively low in this area as compared to the other parts of the world. Yet adequate management might be useful and essential to further minimize the infection in sheep and goats herds. Consequently, preventive measures could further reduce the exposure of toxoplasmosis in the human population. Availability: Items available for loan: UVAS Library [Call number: 0899,T] (1).

24. Occurrence And Importance Of Enteric Protozoan Infections On Different Sheep Farms In District Swat

by Habib Ali | Prf.Dr. Azhar Maqbool | Dr. Kamran Ashraf | Dr. Muhammad | Faculty of Veterinary Sciences.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2005Dissertation note: A study was conducted to determine the prevalence of various enteric protozoan infections in sheep. A total of 225 fresh faecal samples were collected from different sheep farms in District Swat N.W.F.P. These animals belonged to three different age groups i.e. Group I (up to 6 months of age), Group II (7-12 months of age) and Group III (above 1 year of age). Faecal samples were processed for the detection of Eimeria spp. oocysts, Giardia cysts, Cryptosporidium oocysts and other parasites by three different techniques. By Direct smear method, Sheather,s sugar flotation and zinc sulphate flotation technique. A total of faecal samples found positive for Eimeria oocysts 63 (28%), Giardia cysts 35 (15.55%), Cryptosporidium oocysts 16 (7.11%) and mixed parasitic infection 58 (25.77%). Data recorded for various protozoan infections indicated that prevalence rate in Group-I (Eimeria 37.33%, Giardia 22.66% and Cryptosporidium 12%) was higher than the Group-II (Eimeria 28%, Giardia 16% and Cryptosporidium 3%) and Group-III ( Eimeria 18.66%, Giardia 8% and Cryptosporidium 5.33%). Prevalence rate was also higher in diarrhoeic animals (Eimeria 35.71%, Giardia 23.80% and Cryptosporidium 16.66%) than the non diarrhoeic animals (Eimeria 26.22%, Giardia 13.66% and cryptosporidium 4.91%). A large number of faecal samples (25.77%) were found positive for various other parasitic infections. No significant difference was noted in males and females. Results of this study indicated wide spread prevalence of parasitic diseases in sheep population and mostly young animals were found to be infected by different protozoan parasites. It can be concluded that enteric protozoan infections in sheep can be controlled through improved managemental practices, hygienic measures and avoidance of overcrowding the animals. Availability: Items available for loan: UVAS Library [Call number: 0901,T] (1).

25. Toxonomical Study Of The Genus Trichuris And Comparative Anthelminitc Efficacy Of Fenbendazole (Panacur) Ad Albendazole (Valbazen) Against Gastro-Intestinal Nematodes in Sheep

by Dr. Muhammad Iftikhar | Mr. Asif Rabbani | Mr. M. Akram | Mr. Muhammad Afzal | Faculty of Veterinary Sciences.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 1990Dissertation note: Pakistan has a large population of livestock, which plays an important role in the economy of the country. Parasitism is a major problem in our country. Great economic losses have been attributed to the sheep population in our country due to the parasitic infections. The genus Trichuris includes some of the most important nematode species effecting the sheep, therefore a taxonomical study of the genus Trichuris was conducted in the month of September and October, 1989. For this purpose 100 guts (large intestine) of sheep slaughtered at Lahore abattoir were procured and brought to the parasitology laboratory College of Veterinary Sciences, for further investigations. The overall incidence of Trichuris spp. was found to be 74% . The following two species were recorded. 1. Trichuris ovis 66% 2. Trichuris globulosa 18% Taxonomy of Trichuris species was studied by preparing permanent mounts. The present work was also aimed to see the effect of two commonly available anthelmintics namely fenbendazole (panacur) and albendazole (valbazen), on naturally infected sheep with gastrointestinal nematodes. Sixty sheep positive for nematode infections were randomly divided into three groups A,B & C each group comprising of 20 animals. Group A and B were given fenbendazole and albendazole respectively according to the manufacturers dose rate while group C was kept as untreated control. The efficacy of both the drugs was evaluated on the bases of reduction of eggs per gram of faeces after medication. The egg counts were made on zero, 7th, 14th and 21st day. Both the drugs revealed a progressive decrease in the faecal egg counts. The efficacy of fenbendazole on 7th, .14th and 21st day was 96.33, 97.05 and 99.66% respectively while the efficacy of albendazole was 92.56, 95.37 and 98.18% respectively. There was no significant difference in the effectiveness of the drugs. Availability: Items available for loan: UVAS Library [Call number: 1130,T] (1).

26. Studies On Rats And Mice As A Reservoir Of Zoonotic Parasites

by Muhammad Sarfaraz Ahmad | Prf.Dr. Azhar Maqbool | Dr. Muhammad Mustaq-ul-Hassan | Faculty of Veterinary Sciences.

Material type: book Book; Format: print Publisher: 2009Dissertation note: A total of 3600 rats and mice were examined for the prevalence of various zoonotic parasitic infections. Species of various parasites were identified. The order of ifection rate was as Hymenolepis nana (59.9%), Toxoplasma gondii in rats and mice 52.0%), Hymenolepis diminula (35.4%), Cryptosporidium parvum (16.5%), Taenia taenieformis (11.7%), Toxoplasma gondii in humans (11.3%), Capillaria hepatica 7.0%), Babesia spp. (2.3%), Trypanosomu spp. (2.3%) and Leishmania spp. (1.2%). The highest (78.0%) month-wise prevalence of FL nana was found during August whereas the lowest (43.0%) during February. The highest (70.8%) season-wise prevalence was noted during summer followed by autumn (62.9%) then spring whiles the lowest (46.8%) during winter. Infection was higher in males (78.0%) than females 45.9%). Adult rats and mice were more commonly affected than younger. Infection was higher in rats (65.1%) than mice (16.3%). The highest (48.0%) month-wise prevalence of H diminuta was found during August whereas the lowest (28.0%) during January. The highest (45.4%) season-wise prevalence was noted during summer followed by spring (35.1%) while the lowest (29.3%) during winter. Infection was higher in males (43.8%) than females (29.3%). Adult rats and mice were more commonly affected than younger. Infection was higher in rats (37.3%) than mice (20.2%). The highest (23.0%) month-wise prevalence of C. parvum was found during July whereas the lowest (10.0%) during December. The highest (22.4%) season-wise valence was noted during summer followed by spring (16.9%) then autumn (15.3%) le the lowest (11.3%) during winter. Infection was higher in females (19.9%) than es (12.0%). Adult rats and mice were more commonly affected than younger, infection was higher in rats (17.7%) than mice (7.6%). The highest (20.0%) month-wise prevalence of T raenieformis was found during y and August whereas the lowest (6.0%) during December. The highest (18.4%) son-wise prevalence was noted during summer followed by autumn (10.8%) then ring (10.6%) whereas lowest (7.0%) during winter. Infection was higher in females 15.6%) than males (6.5%). Adult rats and mice were more commonly infected than younger. Infection was higher in rats (12.4%) than mice (6.0%). The highest (12.7%) month-wise prevalence of C. hepatica was found during August whereas the lowest (4.0%) during November. The highest (10.8%) season-wise prevalence was noted during summer followed by autumn (6.0%) then spring (6.2%) hues the lowest (4.9%) during winter. Infection was higher in females (8.4%) than males (5.0%). Adult rats and mice were more commonly affected than younger. Infection higher in rats (7.3%) than mice (4.6%). The highest (9.0%) month-wise prevalence of Babesia spp. was found during August whereas infection was absent during January, February, March, April, November and December. The highest (5.0%) season-wise prevalence was noted during summer followed by autumn (4.2%) then spring (0.1%) and was absent during winter. The infection was higher in males (2.7%) than females (2.0%).The infection was present only in adult rats and mice and no case was recorded in younger. Higher infection was noted -n (2.3%) and than mice (2.4%). The highest (7.3%) month-wise prevalence of Trypanosoma spp. was found August whereas it was absent during January, February, November and December. The highest (5.7%) season-wise prevalence was noted during summer by spring (2.2%) then autumn (1.2%) and it was absent during winter. The infection was higher in females (2.5%) than males (2.0%). Adult rats and mice were more commonly affected than younger. Higher infection was noted in rats (2.5%) than mice The highest (4.7%) month-wise prevalence of Leishmania spp was found during y and August whereas infection was absent during January, February, March, April, October, November and December. The highest (3.4%) season-wise prevalence was during Summer followed by autumn (1.1%) then spring (0.1%) and infection was during winter. The infection was higher in females (1 .4%) than males (0.9%). The infection was found only in adult rats and mice and no positive case was recorded in 1younger. The infection was (1.2%) in rats and mice. The role of meteorological data i.e temperature, humidity and rainfall was also studied. It was noted that there was a positive co-relation of high temperature and humidity with the prevalence of various parasitic infections. As the infection were maximum during summer when the temperature and humidity was favorable for high prevalence of parasitic burden. This study will he helpful to disseminate information regarding zoonotic potential parasitic infections via media i.e. newspapers. magazine, electronic media (T.V, I.T, Radio) through seminars, meetings and conferences with professionals doctors, paramedical staff and livestock farmers. Availability: Items available for loan: UVAS Library [Call number: 1149,T] (1).

27. Git Helminthiasis Indigenous And Commercial Layers In District Quetta.Balouchistan

by Rizwan ullah hashmi | Dr. Khalid saeed | Dr. kamran ashraf | Dr. muhammad.

Material type: book Book; Format: print Publisher: 2011Dissertation note: The study was conducted to identify and compare the extent of gastrointestinal helminths in indigenous poultry and commercial layers. For this purpose, 200 gut samples (100 each from indigenous and commercial layers) were collected from different sources of Quetta district. All the material was brought to disease investigation lab, Quetta, for detailed postmortem and coprological examination. The overall incidence of nematodes in indigenous chickens and commercial layers was 87% and 08% respectively. Three species of nematodes were recovered from both indigenous chickens and commercial layers. Ascaridia galli was the most common specie of nematodes recovered from small intestine while Heterakis gallinae and Subulura brumpti were isolated from the caeca of the sampled birds. The incidence of Ascaridia galli, Heterakis gallinae and Subulura brumpti was 75%, 25% and 16% in indigenous chickens and 06%, 04% and 03% in commercial layers respectively. The overall incidence of cestodes was lower than those of the nematodes. It was found to be 78% in indigenous chickens and 05% in commercial layers respectively. Five species of cestodes recorded from the gastrointestinal tract of indigenous chicken and were Raillietina tetragona (60%), Raillietina echinobothrida (21%), Raillietina cesticillus (14%), Cotugnia digonopora (29%) and Choanotaenia infundibulum (13%). On the other hand, following two species of cestodes were isolated from the gastrointestinal tract of commercial layers and included Raillietina tetragona (04%), and Choanotaenia infundibulum (02%). Older birds have higher prevalence as compared with younger birds in indigenous and commercial layers. Slightly higher infection rates were recorded in females (51.7%) as compared with males (48.2%). On coprological examination, a greater proportion of birds were found infected with various helminthes and infection rate in indigenous birds was 66% as compared with commercial layers which was 3%. Availability: Items available for loan: UVAS Library [Call number: 1300,T] (1).

28. Helminthiasis In Children Upto Twelve Years Of Age And Their Effect On Different Blood Parameters

by Dr. Fareed Ahmad Afzal | Prf.Dr. Azhar Maqbool | Dr. Muhammad | Dr. Tayyaba Ejaz.

Material type: book Book; Format: print Publisher: 2011Dissertation note: Helminthic infections causing anemia are the major public health problems globally. Intestinal parasites are most prevalent in tropical countries. Tropical countries have mild climate and heavy rains which are breeding grounds for parasites. The present study was conducted to determine the frequency of intestinal helminthic infections in two to twelve years old children at three different hospitals of lahore. For this purpose, a total of 250 stool samples were examined for the presence of various endoparasites. Stool samples were examined by direct smear and floatation techniques. Identification of eggs was made on the basis of morphological characteristics. 250 blood samples of the same children were also collected for the determination of haemoglobin level. Haemoglobin determination was done by haematology analyzer (Sysmex- model number KX-21). In this way association of parasitic infections with anemia was done. Prevalence of intestinal parasitic infections was noted in children according to age and gender, among different hospitals of lahore and among children whose family members had received different levels of education. The present study has also dealt with the relationship between haemoglobin levels along with their means and standard deviation. Out of the total 250 stool samples examined, it was found that 29 samples were infected with a prevalence of (11.6%). Out of these 29 samples, there were 13(44.82%) cases of Ascaris lumbricoides, 8(27.58%) cases of Hymenolepis nana, 6(20.68%) cases of Taenia saginata and 2(6.89%) cases of Hookworm. The level of haemoglobin in these children ranged from 3.7gm/dl to 9.8 gm/dl. This indicates that there is a relationship between helminthiasis and anemia. Availability: Items available for loan: UVAS Library [Call number: 1334,T] (1).

29. Prevalence, Serodiagnosis And Zoonotic Importance Of Hydatidosis In Small Ruminants And Humans

by Hafiz Javed Iqbal | Prof. Dr. Azhar Maqbool | Dr. Muhammad | Prof. Dr. Athar Khan.

Material type: book Book; Format: print ; Literary form: drama Publisher: 2011Dissertation note: Hydatidosis is a world-wide zoonotic parasitic disease which is caused by the larval stage of tape worm Echinococcus granulosus and E. multilocularis. It is highly endemic in some regions of the world. Keeping in view the importance of the disease, the present study was conducted to record the age, sex, species and season wise prevalence of hydatidosis in small ruminants. The study also includes comparison of the two serological tests used for the diagnosis of this disease and efficacy of mebendazole was aIso evaluated. Prevalence and organ specificity of hydatidosis was studied in 2400 sheep and 2400 goats of different age and sexes. The overall prevalence of hydatidosis in sheep and goats was 8.25 and 6.21 percent respectively. Sex wise prevalence indicated that it was 9.85 and 7.85 percent in female and male sheep respectively whereas in female and male goats it was 5.83 and 7.23 percent respectively. A reasonably higher prevalence of hydatidosis was observed in female animals as compared with males. In adult animals hydatid cysts were present in 11.38 and 7.77 percent in sheep and goatsrespectively while in lambs and kids it was 1.79 and 1.06 percent respectively. Statistically no significant difference was observed in any season through out the year in both the species. In the present study, lungs were found to be most commonly infected organ in sheep andliver in goats. Out of 198 infected sheep, 45.45 percent had cysts in lungs, 33.84 percentin liver, 10.10 percent in both liver and lungs, 2.53 percent in spleen, 2.02 percent in heart and 6.06 percent in abdomen and thoracic cavity whereas in goats organs specificity was 34.23, 40.27, 16.78, 0.67, 8.05 percent respectively for lungs, liver, lungs & liver both, spleen and abdomen and thoracic cavity. A total of 60 cysts in sheep were examined for fertility and it was found that out of 18 cysts of less than two centimeter size, maximum (50 percent) were suppurative orcalcified and minimum (16.7 percent) were fertile in nature. Of 30 cysts of 2-4 em size, 40 percent were suppurative and 40 percent were fertile and from 12 cysts of more than four centimeter size, maximum (66.7 percent) were fertile and only 16.7 percent were sterile in nature. In goats, of 15 cysts of less than 2cm size, maximum (46. 7percent) were suppurative, and minimum (13.3 percent) were fertile. Of 7 cysts, belonging to the group of more than four centimeter seize, 71.4 percent were fertile in nature and only 14.3 percent were sterile. Overall fertility rate in sheep and goats was 38.33 and 36.96 percent respectively whereas overall sterility rate was 23.3 and 32.60 percent in sheep and goats respectively. In sheep, total number of protoscolices found in 23 cysts was 936 of which 72.65 percent were fertile in nature. The mean of total proto scolices was 40.70 with standard deviation of 23.05 whereas the mean number of viable protoscolices was 29.57 percent with a standard deviation of 18.92. In goats, total number of protoscolices observed in 17 cysts was 719 whereas only 52.71 percent were fertile in nature. The mean of total protoscolices was 42.30 with a standard deviation of 17.13 whereas mean of viable protoscolices was 22.30 with standard deviation of 17.10. Blood samples of 40 positive and 40 negative sheep for hydatid disease were collected from slaughter house and serum was separated. ELISA and IHA test were applied on these samples. The sensitivity, specificity, efficacy, positive predictive value and negative predictive value of ELISA were 92.5 percent each whereas these values for IHA were 80 percent, 97.5 percent, 88.75 percent, 96.96 percent and 82.98 percent respectively. In goats, blood samples from 40 positive and 40 negative cases of hydatidosis were collected from slaughter house before slaughtering and ELISA and IHA were applied on all of these samples. The sensitivity and specificity of ELISA was 90 percent and 95 percent respectively whereas the sensitivity and specificity of IHA was 75 percent and 97.5 percent respectively. A total of 12 i.e. 6 sheep and 6 goats (healthy) were purchased and kept at Ravi campus Pattoki, University of Veterinary and Animal Sciences, Lahore. These were divided into experimental (4 sheep and 4 goats) and control group (two sheep and two goats). In experimental group, 100 eggs of Echinococcus granulosus were given orally to all animals and then mebendazole was given to two sheep and two goats on zero day and then after two months intervals. Blood samples were collected from all animals on zero day, 90 days and 180th day and serum was separated. ELISA and IHA were applied to all samples. Mebendazole was not found completely effective and ELISA detected the infection earlier than IHA. On postmortem examination, multiple cysts were found in all animals of group A and no cyst was found in group B. Eighty blood samples of humans i.e. 40 positive and 40 negative for hydatidosis were coilected, serum was separated and ELISA and IHA test were applied to all the samples. The sensitivity and specificity of ELISA was 95 percent and 97.5 percent respectively whereas the sensitivity and specificity of IHA was 82.5 percent and 97.5 percent respectively. The present study will be helpful in disseminating the informations regarding the prevalence, zoonotic importance, effect of mebendazole in animals and the use of immunodiagnostic tests for the diagnosis of hydatidosis in small ruminants and human beings. Availability: Items available for loan: UVAS Library [Call number: 1405,T] (1).

30. Prasitic Contamination Of Vegetables Eaten Raw In Lahore

by Shafa-ul-Haq | Prof. Dr. Azhar Maqbool | Dr. Muhammad | Dr. Nisar Ahmad.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2012Dissertation note: Fresh vegetables are important part of human diet. Eating raw vegetable is customary in many parts of the world including Pakistan, but these raw vegetables can be a major source of parasites. A parasitic survey on vegetables collected from major markets in Lahore was conducted for the discovery of human and animal parasites. Ten species of vegetables: beet (Beta vulgaris), cabbage (Brassica denceal), carrot (Davcus carota) chili (Capsicum frutecense), coriander (Corriandum sativum) cucumber (Cucumis sativa), lettuce (Lactuca sativa), Mint (Mentha viridis), radish (Raphanus sativus), tomato (Lycopersicon esculentun) were evaluated in this study. Fifty samples of each vegetable were taken, comprising a total of 500 samples for the study. Hundred gram of each vegetable was washed with a cationic solution of Hyamin detergent containing glass particles for the elution of eggs. Concentration of eggs/cysts was achieved by centrifugal sedimentation technique at 5000 rpm for 5 minutes. Sediment was examined under microscope for the presence of parasite eggs, cysts and larvae. Examination of vegetables revealed twelve genera of parasites. All vegetables were highly contaminated with parasites with an overall prevalence of 31.2%. Of parasites studied, Ascaris eggs found to be the highest (37.1%), followed by Hook worm(10.8%) and Trichostrongyloides sp.(8.9%), while the least common parasite was Toxoplasma gondii (1.9%). Lettuce showed the highest contamination (48%), followed by Cabbage (44%) and Mint (podina) (42%) while chili showed the least contamination (16%). The results would seem to indicate that one of the important routes of parasitic infection is due to consumption of raw and unwashed vegetables. So, enlightenment programs for the public on necessity of food sanitation and personal hygiene should be intensified. Availability: Items available for loan: UVAS Library [Call number: 1419,T] (1).

31. Prevalence And Control Of Gastrointestinal Nematodes Of Goats In District Swat, Khyber Pakhtunkhwa

by Habibun Nabi | Dr. Khalid Saeed | Dr. Aneela | Dr. Muhammad Lateef.

Material type: book Book; Format: print ; Literary form: drama Publisher: 2012Dissertation note: Goat rearing is a traditional activity in peri-urban areas and villages and plays an important role in the nutrition, social and economic needs of the population. Gastrointestinal nematodes are common in various production systems and these infections are the single most significant constraint to small ruminant productivity. The present study was carried out at Saidu Sharif, District Swat to generate data about the gastrointestinal (GIT) nematodes prevalent in the area. To record the prevalence of various nematodes of goats, a total of 150 faecal samples were examined. A total of 61/150 (40.67 %) of samples were found positive. Different species were identified and included Nematodirus spathiger (28.66 %), Haemonchus contortus (14.66 %), Trichostrongylus spp (4 %), Strongyloides papillosus (6 %) and Trichuris ovis (11.33 %). Highest prevalence of GIT nematodes were found in young and immature animals (48 %) with mean EPG 282 and 211 respectively. Adult had lower infection rate (26 %) with mean EPG of 142. Sex related prevalence indicated that male had higher prevalence (46.66 %) than female (34.66 %) and greater intensity of infection in males as compare to females. Higher infection rate with GIT nematodes was recorded in pregnant goat (42 %) as compared to non pregnant (33.92 %). House hold animals showed high infection rate i.e. 45.33 % with mean EPG of 254 as compared to commercially raised animals which had infection rate 36 % with mean EPG of 169. The Study -------------------------------------------------------------------------------------------------- SUMMARY 71 showed significant difference of egg per gram count between breeds. Beetal goats had high infection rate (100 %) and intensity as compared with other breeds. Higher egg shedding was recorded in animals around parturition as compared to dry goats. Liter size also effected the EPG and higher counts were recorded in goats with duplets and triplets. Albasym (Albendazole) was evaluated at two dose levels i.e. 1 ml/ 20 kg body weight and 1.25 ml/20 kg body weight. The efficacy at normal dose and higher dose at day 7th was 98.81 % and 100 % respectively, at day 14th 95.24 % and 99.53 % and at day 28th 80.95 % and 93.90 % respectively. No significant difference at normal dose and higher dose was observed while significant difference with control group was recorded. Availability: Items available for loan: UVAS Library [Call number: 1435,T] (1).

32. Adulticidal And Larvicidal Activity Of Cassia Fistula And Piper Nigrum Against Anopheles (Malaria Vector).

by Sara Mehmood | Dr. Muhammad Lateef | Dr. Aftab | Dr. Khalid Saeed.

Material type: book Book; Format: print ; Literary form: drama Publisher: 2012Dissertation note: Mosquitoes are major vectors, in transmitting many diseases like malaria, filariasis, Japanese encephalitis and dengue fever etc, which are greatly, affecting human beings worldwide. Malaria is a major global health problem. There are estimated 247 million malaria cases with almost half of the global population at risk and nearly a million deaths each year. Several Anopheles species are responsible for transmission of malaria .i.e. Anopheles stephensi, An. Subpictus. Control of mosquitoes primarily depends on chemical insecticides includes organochlorine, organophosphates, synthetic pyrethroids, IGRs etc, but all these chemicals have developed a feedback of environment ill-effect, have negative impact on non-targeted biota and most mosquitoes species have become physiologically resistant to these insecticides. These problems have highlighted the need for the development of new strategies for mosquito control. Botanical insecticides are naturally occurring products that are derived from plants. The use of plant extracts for insect control has several appealing features, as these are generally more biodegradable, less hazardous, and rich storehouse of chemicals of diverse biological activity. The current study were carried out to evaluate the activity of methanol extract of leaves of C.fistula, and ripened fruits of P. nigrum against Anopheles mosquito. Among both of these plants the methanolic extracts of Piper nigrum (black pepper) were exhibited remarkable adulticidal and larvicidal potentials. The percentage mortalities were increased by gradual increase in extracts concentrations. Larvae were more susceptible to these methanolic pepper extracts than adults. Larvae were showed mortalities even at very low concentrations. The LC50 values obtained by using probit analysis were 25.05 ppm and 12.05 ppm after 24 hrs and 48 hrs respectively, whereas LC90 were 78.63 ppm and 53.06 ppm respectively for aulticidal bioassays. Larvicidal assays were also showed good results having LC50 values as 3.87 ppm and 2.07 ppm after 24 hrs and 48 hrs respectively, whereas LC90 were 10.63 ppm and 6.56 ppm respectively. Cassia fistula (golden shower) leaf extracts were also showed promising mosquitocidal efficacy against Anopheles stephensi. LC50 values obtained by using probit analysis were 35.13 ppm and 16.18 ppm after 24 hrs and 48 hrs respectively, whereas LC90 were 94.57 ppm and 76.43 ppm respectively for aulticidal bioassays. Larvicidal assays were also showed good results having LC50 values as 44.99 ppm and 24.40 ppm after 24 hrs and 48 hrs respectively, whereas LC90 were 106.12 ppm and 76.30 ppm respectively. It is concluded that methanolic extracts of these plants Cassia fistula and Piper nigrum have high potential of adulticidal & larvicidal activities. So the extracts of these plants can be used as an alternatives to the conventional insecticides for long lasting mosquito problems as these are less hazardous and ecologically feasible. However, a lot of work on biochemistry as well as insectidal activity and on other aspects has to be done. Availability: Items available for loan: UVAS Library [Call number: 1474,T] (1).

33. Serofpidemiology, Zoonotic Potential And Chemotherapy Of Neosporosis In Dogs And Cattle

by Muhammad Mudasser Nazir | Prof. Dr. Azhar Maqbool | Dr. Muhammad Lateef | Prof. Dr.

Material type: book Book; Format: print ; Literary form: drama Publisher: 2010Dissertation note: The aim of current study was to demonstrate the most important features of Neospora caninum infection in Pakistan. In the present study, I examined the prevalence of N. caninum in 7 districts of the country and to accessed the efficacy of various drugs against the parasite in cell culture. For the achievement of this purpose, the core objectives were, To have an overview on the overall seroprevalence of neosporosis throughout the country by means of cELISA in aborting, at risk and clinically healthy cows. To check the correlation of Iscom ELISA and cELISA, and determination of prevalence of N. caninum by means of Iscom ELISA on milk samples. To identify the transmission of disease towards human. To determine the efficacy of various drugs against N. caninum. In phase 1, overall seroprevalence of N. caninum in dairy cattle (detected by means of cELISA, VMRD, Inc., Pullman, WA, USA) was found to be 43.4% with a significant difference (P < 0.05) of seropositivity among all 18 herds (n = 5 aborting herds, n = 13 non-aborting/clinically healthy herds) selected from 7 districts of Pakistan. The seropositivity of cattle to N. caninum antibodies was significantly higher in aborting animals (52.7%) as compared to non-aborting cows ( 41.5%), indicating a significant difference between aborting and non-aborting cattle. In case of pregnant and non-pregnant animals, similar findings were recorded in our study. A significantly higher rate of seroprevalence was observed in pregnant dams (59.8%) than non-pregnant cattle (35.2%). Overall, higher serological prevalence was evaluated during the summer season (61.1%) in all areas followed by autumn (46.9%), spring (34.9%) and least seropositivity was observed in winter season (26.6%). The difference in seropositivity was significantly different among all age groups, greater in animals older than 2 years of age. Furthermore, the prevalence was statistically significant (P < 0.05) among cattle of different breeds. Seroprevalence in cases of crossbred animals were higher followed by exotic and indigenous breeds. Phase 2, describes the seroprevalence of N. caninum in clinically healthy dairy cows. A selection of 760 animals from 13 dairy herds located in Punjab and Sindh Province, Pakistan to demonstrate the presence or absence of the Neospora caninum infection in commercial dairy cattle. The serostatus of the cows towards N. caninum was detected by cELISA (VMRD, Pullman, WA). Out of 760 animals, (43.2%) were seropositive to N. caninum. A significant difference of positivity was recorded among all 13 dairy herds. Age wise prevalence though not statistically significant among all age groups, was greater in animals over 2 years of age and least in heifers. Variation was also observed in samples from cattle of different breeds. A significantly higher prevalence was observed in crossbred animals than in purebred and nondescript cattle. Seasonal prevalence was higher during summer season than rest of the seasons. The seroprevalence of N. caninum in pregnant cows was significantly greater than in non-pregnant animals. Second experiment of this phase describes the seroprevalence of 240 animals from 5 herds with a high rate of abortion, the percentage of seropositivity observed in these herds was 43.8%, slightly higher than the clinically healthy and non-aborting cattle. No significant difference was observed among all sample locations in this experiment. However, significant difference of positivity was recorded among different breeds of cattle. Age wise prevalence, though not statistically significant (P > 0.05), was greater in animals older than 2 years of age. The assessment of milk samples from lactating cows were also determined for Neospora caninum antibodies by means of Iscom ELISA (SANOVIR® Sanova Biotech AB, Uppasala, Sweden) and showed a good level of agreement (r² = 0.9959) between the two tests (cELISA and Iscom ELISA). Although, the cELISA (VMRD, Inc., Pullman, USA) expressed a higher seropositivity and sensitivity than Iscom ELISA (Sanova Biotech AB, Uppasala, Sweden). Therefore, both of the ELISA tests (cELISA and Iscom ELISA) for the detection of N. caninum antibodies in dairy cattle can perform better in lactating animals. The Iscom ELISA has some advantages over cELISA as it's easy to collect milk samples than serum samples, moreover Iscom ELISA is cheaper and easy to use but has low sensitivity than cELISA and cannot be used in dry animals. The percentage of positivity detected through Iscom ELISA on individual milk samples were 61.4% and 76.6% by cELISA. In phase 3, an epidemiological study was conducted to determine seroprevalence of N. caninum in dogs of different breeds and age groups. The serum samples of dogs were analyzed by cELISA (VMRD, Pullman, USA) showed a seropositivity of 23.5%. There was no significant difference of seropositivity among various sample locations, highest prevalence was observed in Muzaffar Garh (31.9%), followed by Gujranwala (27.9%), Lahore (25.1%), Hafizabad (20.2%) and least prevalence was recorded in district Okara (14.6%). A significant difference in prevalence of N. caninum antibodies between male (26.1%) and female (18.8%) dogs were recorded. The difference in seroprevalence was not significant among all age groups. The samples with no age record showed a highest prevalence (29.5%) and least seropositivity was observed in adult dogs of 3-6 years of age (18.7%). During Summer season, highest positivity to N. caninum was (31.0%) recorded while the lowest prevalence (16.0%) was observed in Winter season. Phase 4, describes the seroprevalence and transmission of N. caninum in humans. A selection of 52 serum samples from humans was analyzed for the presence of N. caninum antibodies. The serostatus of the humans towards N. caninum antibodies (IgG) was determined by using commercially available antigen coated IFAT slides (VMRD, Inc., Pullman, Washington USA) and human conjugate. Overall very low prevalence (1.9%) of N. caninum antibodies was reported in this study. Only one case was found to be positive, these findings indicate that no strong evidence of N. caninum infection in humans. In phase 5, in vitro drug trials was conducted to access the best efficacy of three commercially available drugs. We found that among three anticoccidial drugs i.e Clindamycin, Diclazuril and Sulfadiazine, Diclazuril has best inhibitory effect against N. caninum tachyzoites in cell culture followed by Clindamycin and sulfadiazine. Availability: Items available for loan: UVAS Library [Call number: 1575,T] (1).

34. Epidemiology, Zoonotic Potential, Serodiagnosis And Chemotherapy Of Sheep Fasciolosis In Different Ecological zones of balochistan

by Masood Ul Haq Kakar | Prof. Dr. Azhar Maqbool | Dr. Muhammad | Prof. Dr. Yasmeen Nawaz.

Material type: book Book; Format: print ; Nature of contents: biography; Literary form: Publisher: 2012Dissertation note: Various epidemiological aspects of human and sheep fasciolosis were investigated in four districts of Balochistan (Pakistan) having different ecology i.e. district Bolan from (Plain zone), Lasbela (Coastal zone), Qilla Saifullah (sub humid and semi arid sub zone of Upland zone) and district Pishin from (Arid sub zone of Upland zone). Sheep samples were examined through Coprological examination showed overall prevalence of 10.26% in one year study period from June 2010 t0 May 2011. The uppermost prevalence was recorded in district Bolan (14.79%) followed by Lasbela (10.63%), Qilla Saifullah (8.75%), and the lowest in district Pishin (6.88%). Overall the highest prevalence by season was recorded in autumn (25.31%) followed by winter (9.22%), summer (6.41%) and lowest in spring (5%). Amongst the month the overall highest prevalence was recorded in the month of September (30.63%) and lowest in the month of May (1.88%). Sex wise prevalence was found highest in female more susceptible to infection (11.22%) than male (8.48), but sex wise difference was non-significant statistically. Amongst the age group significantly higher prevalence was recorded in adults young than adult of age group (5.91%). During one year study period prevalence (%) of human fasciolosis in some districts of Balochistan was recorded (0.42%), with overall district wise prevalence in Qilla Saifullah and Bolan (0.83%) and (0%) in Lasbela and Pishin. Overall season wise prevalence was noted the highest in autumn (1.25%) followed by summer (0.63%) and 0% prevalence in winter and spring. Month wise results showed 2.5% prevalence only in the month of August and October while 0% in the other months. Gender wise prevalence 0.42% was found only in male, no female samples were collected due to some religious, traditional and community problems. Prevalence by age was recorded the highest in above 20 years of age group (0.74%) while this value decreased to zero in below 20 years of age group. Antibodies against fasciolosis in serum samples through indirect (ELISA) were recorded 13.13% (63/480) in sheep and 0.42% (2/480) in human indicates the higher prevalence (%) as compared to fecal examination. Likewise district, age and sex wise seroprevalence (%) of fasciolosis was reported higher than coprological examination in case of humans as well as in sheep. In sheep positive correlation was noted between fasciolosis and relative humidity while negative correlation with temperature (ºC) and rainfall (mm). While in humans prevalence positive correlation was observed with temperature (ºC), relative humidity (%) and rainfall (mm). Overall 1123 snails belonging to different 5 genera were collected from different district from different agr-ecological zones of Balochistan from June 2010 to May 2011. Amongst the snails the highest prevalence (37.04%) was found for Indoplanorbis, followed by Bulinus (32.15%), then Lymnea (20.66%), Melanoides (5.52%) and the lowest Physa (4.63%). Comparative study for coprological and serological tests (ELISA) was conducted for four districts from different agro-ecological zones of Balochistan i.e. District Bolan from (Plain zone), Lasbela (Coastal zone), Qilla Saifullah (sub humid and semi arid sub zone of Upland zone) and district Pishin from (Arid sub zone of Upland zone) for one year i.e. from June 2010 t0 May 2011. Overall prevalence of sheep and humans was 0% and 8.13% by coprological examination and 13.13% and 0.42% by indirect ELISA tests. Prevalence by ELISA was found higher than fecal examination when analyzed statistically. Similar seroprevalence for month, districts, age and sex was noted higher than coprological examination for sheep and humans. ELISA Sensitivity (%) and specificity (%) was recorded >97.0% and 95% and 100%, 100%, respectively for sheep and humans. Indigenous plants i.e., Saussurea lappa (roots), Fumaria parviflora (aerial) and Caesalpinia crista (seeds) were used at dose level of 60, 70 and 80 mg/kg body weight against naturally infected sheep with fasciolosis and their effectiveness was compared with triclabendazole (10mg/kg body weight). Triclabendazole was found 100 % effective after second dose whereas all herbal medicine it reached up to this mark after administration of second dose of 80 mg/kg body weight. From this study we can conclude that these herbal medicines can safely replace the triclabendazole, which is not, only cost effective but have no side effects. Availability: Items available for loan: UVAS Library [Call number: 1587,T] (1).

35. Assessment Of Parasitological Contamination Of Fresh Ad Dry Fruits In Lahore

by Muhammad Anis Khan | Dr. Nisar Ahmad | Dr. Muhammad | Prof. Dr. Azar Maqbool.

Material type: book Book; Format: print Publisher: 2013Dissertation note: Fruits are important part of human diet. Eating unwashed fruits is common in many parts of the world including Pakistan, but these can be a major source of parasites. A parasitic survey on fruits collected from major markets in Lahore was conducted for the discovery of human and animal parasites. Ten species of fruits: Guava, Apple, Sapodilla, Sweetsop, Strawberry and dry fruits: Apricot, Date, Persimmon, Fig and Raisins were evaluated in this study. Fifty samples of each fruit and a total of 500 samples were taken for the study. Hundred gram of each fruit was washed with a cationic solution of Hyamin detergent containing glass particles for the elution of eggs. Concentration of eggs/cysts was achieved by centrifugal sedimentation technique at 5000 rpm for 5 minutes. Sediment was examined under microscope for the presence of parasite eggs, cysts and larvae. Examination of fruits revealed nine genera of parasites. All fruits were highly contaminated with parasites with an overall prevalence of 35.2%. Of parasites studied, Ascaris found to be the highest (36%), followed by Trichuris (14.3%) and Trichostrongyloides sp.(11.9%), while the least common parasite was Fasciola (2.9%). Fruits are the source of parasitic infestation. The most contaminated fresh fruit was strawberry (54%) followed by guava (48%). Whereas the least contaminated fresh fruits was sweetsop (22%). The most contaminated dry fruits was fig (46%) followed by date (38%). whereas the least contaminated dry fruits was (20%). The results indicate that one of the important routes of parasitic infection is due to consumption of unwashed fruits. Conclusions: Strong efforts should be made to prevent contamination of fruits and vegetables during production, transport, processing and handling, much improvement is still needed in our country if hygienic production of fruits and vegetables is to be ensured. This study provides an overview of the hazards associated with eating contaminated fruits. Parasitic infections like Cryptosporidiosis can be acquired by people if orchards or water sources near cow pastures become contaminated from infected cows and people consume the fruit without proper washing. The study needs to be carried out in other areas of Pakistan and more fresh and dry fruits should be included in the study. Information regarding the contamination of the fruits should be published in the national newspapers because awareness of the public is very important for the control of these contaminations. Availability: Items available for loan: UVAS Library [Call number: 1645,T] (1).

36. Prevalence Of Defferent Developmental Stages Of Aedes Mosquitoes And Their Role In Transmission Of Dengue

by Sabila Afzal | Prof. Dr. Azhar Maqbool | Dr. Aftab A | Dr. Muhammad Latif.

Material type: book Book; Format: print ; Literary form: drama Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1735,T] (1).

37. Isolation Of Local Strain Of Toxoplasma Gondii Through In-Vivo Cultivation In Mice

by Rahim Gul | Dr. Muhammad Imran Rashid | Dr. Aneela | Dr. Nisar Ahmad.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2013Dissertation note: Toxoplasma gondii is an obligate apicomplexan, intracellular, parasite that infects all warm-blooded vertebrates, including mammals and birds. Human beings can be infected by ingestion of oocysts from cat faeces or through the consumption of meat containing Toxoplasma gondii cysts. Thus, food animals can be the source of transmission of Toxoplasmosis in human population especially among people who consume undercooked meat in the forms of barbecues, beef steaks, kebabs, burgers and shawarmas. Oocysts of T. gondii from cat faeces were identified by using direct microscopy and flotation technique. The positive oocysts were confirmed by micrometry having diameter of 9-13 ìm. The oocysts were then sporulated in aerated condition. After sporulation oocyst were inoculated in Swiss albino mice for in-vivo culturing. After 56-70 days brain tissue was collected from infected mice and subjected to DNA extraction and PCR amplification. Similarly DNA was also extracted from sporulated oocyst for copro-PCR. Out of 200 faecal samples only three were found positive for Toxoplasma gondii through direct microscopic examination and flotation technique. From positive faecal sample and brain tissue DNA was extracted by QIAGEN mini stool kit and QIAGEN DNA mini kit. After DNA extraction the samples were examined through PCR by using specific Toxoplasma gondii B1 gene primer having 529 bp size. Two hundred faecal samples were examined for T. gondii using direct microscopy, flotation technique, bioassay and polymerase chain reaction. Out of 200 samples 3 (1.5%) were found infected through direct microscopy and flotation technique. Toxoplasmosis was more prevalent in adult cats (1.65%) as compared to young ones. Prevalence was also found high in females (2.08%) as compared to males. Similarly healthy cats have higher prevalence rate (1.30%) as compared to diseased ones. A further confirmation was done through polymerase chain reaction and brain tissue cyst Bioassay give 1 positive amplification while Copro-PCR gives 2 positive amplifications. Therefore it can be concluded that the copro-PCR is can be used for the confirmation of Toxoplasma oocysts from cat faeces and tissue cysts from bioassay in mice. Therefore, we propose that the copro-PCR can be used as the new gold standard for determining potential cat infectivity and tissue cysts from bioassayed mice or contaminated meat samples of livestock. Availability: Items available for loan: UVAS Library [Call number: 1778,T] (1).

38. Comparative Efficacy Of Different Diagnostic Techniques For Ovine Haemonchosis Through Faecal

by Sadaf Anwar | Dr. Muhammad Lateef | Dr. Aftab | Prof. Dr. Azhar Maqbool.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2013Dissertation note: Livestock plays pivotal role in the economy of country and small ruminants are the major source of food products for human. Haemonchus contortus is the most significant parasite of small ruminants and cause heavy production losses by causing reduction in meat and wool production. The parasite directly affects the health of an animal and causes anemia, hemorrhages, anorexia, weight loss and death of affected animal. This study was designed to diagnose the Haemonchosis in sheep in and around Lahore. The accurate diagnoses of the parasite are important for its control and treatment. Direct smear, floatation technique and Polymerase Chain Reaction (PCR) were applied to check the sensitivity and specificity in diagnosing Haemonchosis in sheep. For this purpose 100 faecal samples were collected randomly from different areas of Lahore. Each faecal sample was examined by direct smear method and floatation technique. Out of 100 faecal samples 44 were microscopically positive. 30 by direct smear method and 44 by floatation technique. Specific primers were designed to diagnose Haemonchosis in sheep by using ITS-2 Region. 44 microscopically positive samples were confirmed by PCR. 29 (66%) samples were found to be PCR-positive and 15 (34%) were found to be PCR-negative. Several other species of parasites were also found during microscopic examination of faecal samples. Two samples wetre found positive for coccidial oocyst and seven samples were also positive for other nematodes along with Haemonchus spp. Flotation technique was found to be superior in diagnosis of Haemonchosis as compared to direct smear. Conclusion: From current study it is concluded that Haemonchosis is widely prevalent in ovines. Its accurate diagnosis is essential for the treatment of infectious diseases and control of this parasite. Molecular technique has the advantage over conventional diagnostic techniques because PCR is more specific than conventional methods of diagnosis. According to the present study by conventional method there is 34% error chances to diagnose other Trichstrongylid eggs as Haemochus spp. The main advantage of using PCR as diagnostic test, are an increased speed of diagnosting the disease and its capability to notice low worm burden in small volume of faeces from individual animals. Availability: Items available for loan: UVAS Library [Call number: 1809,T] (1).

39. Prevlence Zoonotic Potential Water Borne Transmission And Chemotherapy Of Cryptosporidiosis In Small Ruminants

by Muhammad Abubakar shafiq | Prof. Dr. Azhar Maqbool | Dr. Muhammad | Dr. Muhammad Lateef.

Material type: book Book; Format: print Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1910,T] (1).

40. Detection And Chemotherapy Of Demodex Mite In Dogs In And Around Lahore

by Asim rasool | Dr. Nisar ahmad | Dr. Muhammad Haroon akber | Prof. Dr.

Material type: book Book; Format: print ; Nature of contents: biography; Literary form: Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2019,T] (1).

41. Phylogenetic Analysis Of Haemoproteus In Chicken And Sparrows

by Anha fatima | Dr. Muhammad imran rashid | Dr. Azhar maqbool | Dr. Wasim.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2038,T] (1).

42. Optimization Of Loop Mediated Isothermal Amplification Lamp Technique For The Molecular Diagnosis Of Surra In Domestic Animals

by Muhammad Saleem iqbal | Dr. Haroon akbar | Dr. Muhammad lateef | Prof. Dr. Asim.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2039,T] (1).

43. Phylogenetic Analysis Of Plasmodium Species In Sparrows And Domestic Chicken

by Ghanwa ahmad | Dr. Haroon Akbar | Dr. Muhammad lateef | Prof Dr. Aftab.

Material type: book Book; Format: print Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2075,T] (1).

44. Prevalence And Chemotherapy Of Canine Babesiosis

by Faisal Shrif | Dr. Nisar Ahmad | Dr. MUhammad | Prof. Dr. Azhar Maqbool.

Material type: book Book; Format: print Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2117,T] (1).

45. Epidemiology Zoonotic Potential Haematology And Control Of Amoebiasis In Dogs And Humans

by Muhammad Azhar Alam | Prof. Dr. Azhar Maqbool | Dr. Muhammad Lateef | Prof. Dr.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2130,T] (1).

46. Prevalence And Chemotherapy Of Trichostrongyloids In Camels In Charsada

by Muhammad Qasim | Prof. Dr. Azhar Maqbool | Dr. Muhammad | Prof. Dr. Kamran Ashraf.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2131,T] (1).

47. Development Of Molecular Tools For The Diagnosis Of Plasmodium Vivax Using Cytochrome C Oxidase Gene

by Ayaz Shaukat | Prof. Dr. Azhar Maqbool | Dr. Muhammad | Dr. Muhammad Imran Rashid.

Material type: book Book; Format: print Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2153,T] (1).

48. Molecular Diagnosis Of Feline Babesiosis

by Muhammad Younus Khan | Dr. Haroon Akbar | Dr. Muhammad | Dr. Muhammad Lateef.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2168,T] (1).

49. Detection Of Falciparum Malaria And Its Control Under Local Climatic Conditions

by Muhammad Oneeb | Prof. Dr. Azhar Maqbool | Dr. Muhammad Lateef | Prof. Dr.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2180,T] (1).

50. Epidemiology Zoonotic Potential Haematology Amd Chemotherapy Of Sarcoptic Mange In Camel In Punjab

by Muhammad Irfan Zahid (2011-VA-800 | Prof. Dr. Azhar Maqbool | Prof. Dr. Muhammad Sarwar Khan | Prof. Dr.Shazia Anjum | Prof. Dr. Kamran Ashraf.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2015Dissertation note: A camel is a very hardy ruminant animal, which can survive under harsh climatic conditions very effectively by utilizing the marginal areas with excellent capabilities and produce under such conditions (Hjort and Hussein, 1986; Abbas and Tilley, 1990). Camel is an important animal as it is well adopted in unique manners in the hot, arid and semi-arid environments (Schwartz, 1992). It can survive without water and food for many days and this unique ability of camel makes it an ideal for such harsh conditions for which it is also commonly known as “The Desert Ship”. In spite of the fact that camel is an important member of a group of animals which produces food for human consumption in the shape of milk and meat, yet it is the most neglected one in the field of scientific research. It may be due to the fact that camel belongs to such areas of the world which are arid, semi-arid or rain fed in nature, having harsh climatic conditions, where poor nutrition and poor management are the major issues (Sohail, 1983). It is an established fact that diseases originating from parasites lead to the main health hazard issues in animals. These parasites survive at the expense of the host animals causing lot of health problems, like skin irritation, anemia leading to weakness and debility. Some of the parasites have zoonotic importance and may become a source for the transfer of many contagious diseases like scabies to the human beings (Dominguez et al. 1978). McClain et al. 2009, observed the scabies as a major health problem globally both for humans and animal population. Sarcoptes scabiei is an ectoparasite which is a cause of scabies, a skin problem in the human beings worldwide and the similar species of mites do also produce a similar type of disease in a large variety of wild and domesticated mammals (Pence and Ueckermann, 2002; Fitzgerald et al. 2004). Fain, 1978, reported that more than fifteen (15) different species of Sarcoptes scabiei morphologically and genetically distinct from each other have been identified in different hosts. Introduction 2 Sarcoptic mange is the second important problematic disease of camel after Trypanosomiasis (Nayel and Abu-Samra, 1986). Scabies caused by Sarcoptes scabiei var cameli is a serious & highly contagious skin problem and also economically important disease of the camels (Pegram and Higgins, 1992). Camels, which are reared with deficient nutrition, poor management and under unhygienic conditions are mostly affected by this disease (Kumar et al. 1992). A large group of people and communities living in arid diverse ecozones in the entire world, particularly in harsh climates earns their livelihoods by depending on camels. This dependence may spread to the utilization of camel milk, meat, wool and leather besides its use in transportation, riding and sports (Wilson, 1984; Snow et al. 1992). In Pakistan camels are also raised by the people for meat, milk, riding, transportation and sports purposes in the deserts, semi desert & rain-fed / warm areas of the entire country being a hardy animal as it can tolerate easily the rugged climate as well as extremes of temperatures of such areas. The natural harsh and adverse climatic conditions, particularly during long dry seasons lead to a paucity of feeding regimes resultantly the camels raised in such areas are subjected to stress conditions which lower their resistance and make them easily vulnerable to diseases (Abbas et al. 1993; Agab, 1993). Abbas & Tilley, 1990; Saint-Martin et al. 1992; Abbas and Agab, 2002; Pathak and Chhabra, 2010; while reviewing the parasites & parasitic diseases of camel population in India were of the opinion that Sarcoptic mange is a serious, debilitating, dreaded and widely prevalent disease of camels in India. Besides other infectious diseases of bacterial and viral origin, camels are exposed to a wide range of internal & external parasitic infestations. Amongst other so many external parasites to which camels are exposed, the Sarcoptic mange is recognized to be one of the most Introduction 3 serious and damaging disease. This disease is caused by a mite known as Sarcoptes scabiei var cameli which belongs to genus Camelus of SARCOPTIDAE family in Veterinary Entomology. It is an extremely pruritic, contagious and debilitating skin disease which is very frequently and sudden in onset. It is also ranked as one of the most serious and important disease of the camels. Sarcoptic mange infestation is very common in the areas of thin skin, the head, neck, flanks, medial aspect of thighs or inguinal region, mammary glands and prepuce. The head is usually affected very rapidly as the animal uses its teeth for scratching the affected areas. Besides linking the occurrence of the disease with poor camel management, malnutrition and contact with infected objects, the stray & infected camels also often become a focus of infecting the healthy animals when mingling with them particularly at watering places for drinking purpose (Richard, 1987; Abdel-Rehman et al. 2001). Sarcoptes is a burrowing mite as it penetrates deeply through the skin surface of the infected camel. This burrowing of mites in the skin helps these parasites lead to intense pruritus and exudative dermatitis. In pruritus, mites penetrate deep into muscular areas, damaging the flesh and lowering the quality of meat. The early inflammatory reaction of the host body towards the mites becomes evident in the shape of small popular elevations, invasion and injuries leading to formation of hairless areas, scaly crust formation or scabs on the affected parts and the skin become dark and thickened. Skin of mangy camel show hemorrhages, and subcutaneous odema after the development of fissures in the underlying epidermis (Kumar et al. 1992; Amer et al. 2006). The fertilized female mites create winding burrows or tunnels in the upper layers of the epidermis of the skin of the host animal and feeding on the serous exudate, a liquid oozing from the damaged tissues. The female mites lay about 40-50 fertilized eggs in these tunnels which Introduction 4 hatch in 3-5 days into a six legged larvae. These larvae immediately crawl to the surface and burrow themselves in the superficial layers of the skin and create small molting pockets. In these molting pockets, the larvae molt to next stages of nymph and adult. The adult male then emerges and seeks a female either in the molting pocket or on the surface of skin. After fertilization the female produces new tunnels, either de novo or, by extension, of the molting pockets, lays eggs in these tunnels and a new life cycle starts. The entire life cycle of Sarcoptic mange is completed in 17-21 days. New hosts can be infected through direct transmission by contact between the animals, presumably from larvae, nymph or adult mites, which are commonly present on the skin surface of the infected animal. Indirect transmission of infestation can also take place through the objects or fomites having mange infection, which come into contact with the affected camel, such as harnesses, blankets, baggage tack, tents and tree trunks (Richards, 1987). The pruritus increases as the mites penetrate deeper in the skin (Al-Rawashdeh et al. 2000, Driot et al. 2011, Bekele et al. 2012). Based on the rate of infection camels can be seriously disturbed by the Sarcoptic infestation as they may stop grazing which can lead to a rapid fall in milk production, and deterioration of health condition. With the increase in the irritation due to scabies, the camel rubs, bites and scratches the affected areas in an attempt to reduce the itchiness. Due to rubbing, biting or scratching, the mites move to the periphery affecting the healthy tissues and resultantly affected area spreads. As the disease prolongs, the skin becomes excoriated, leading to hair loss and the development of scabs. These scabs in turn may be rubbed away and a red surface developed. The animal becomes restless due to severe Sarcoptic mange infestation and involvement of most of the body surface. If the diseased animal is not treated in time, the animal loses its health condition, become emaciated and within two, three weeks the acute stage of Introduction 5 disease may give way to more chronic state (Gorakh et al. 2000, Abubakar et al. 2002, Driot et al. 2011). Sarcoptic mites rarely survive long off the host under natural conditions. A continuous direct contact of animal keepers with their camels can also lead to transmission of diseased condition in human beings which is termed as pseudo scabies. Transmission of infection from camel to man usually takes place during milking, handling or riding. The main symptoms of pseudo scabies can therefore be seen in the inter digital spaces of the hands, on the wrists, forearms, the elbows, the axillary folds and inner side of the thighs. Once a herd is infected with Sarcoptic mange, continuous reinfection of the disease occurs (Schillinger 1987, Singh & Veer 2005, Premalatha et al. 2010). Sarcoptic mange is usually considered to be a seasonal disease and is often reported severe during the winter months as in cold weather the disease had an acute course. However, there is some evidence that in some countries hot weather predisposes to acute outbreaks of camel mange and in the cooler, winter season the rate of mange infestations are at the lowest. In the summer the activity of the mite seems to decline or disease becomes chronic. Dietary intake is an important factor in mange infestation. Nomadic camels on a low nutrition plan, probably carrying heavy worm burdens in hot desert conditions are likely, therefore, to be highly prone to Sarcoptes at this time (Dinka et al, 2010). During such periods of great activity, the mites are readily transmissible from one animal to other animals (Richards, 1987, Banaja & Ghandour, 1994, Tefera & Gebreah, 2001). Mange can easily be diagnosed clinically from the occurrence of pruritus, depilation, alopecia, thickened skin, folds around the joints and encrusted plaques being the main characteristics of this parasitosis. In order to control this zoonotic disease, it is essential to treat Introduction 6 both camel and man along with effective checks over other predisposing factors of the disease such as hygiene and nutritional requirements of the animals. The skin diseases like the scabies both in human beings and animals are being treated with a variety of allopathic drugs now a day, but the role of herbal plants in use since centuries in different shapes cannot be ignored at all, especially in the rural lifestyle. Further with the continuous use of different acaricidal drugs, the issue of resistance development has come across as a challenge for the researchers to find some alternatives for the purpose. Accordingly the research work on the use of traditional herbal medicines is gaining attention day by day. Although there are many reports and studies regarding the prevalence of Sarcoptic mange in camel from different parts of the world, only few preliminary reports are available for Pakistan and none of them provide detailed epidemiology of Sarcoptic mange and its effect on host health. Therefore, keeping in view the importance of the mange problem in camel population of the country, the present project was designed to determine the prevalence of Sarcoptic mange infestation, factors in its occurrence its zoonotic importance, effect on blood physiology and different treatment options in the camel population of Punjab, province in Pakistan. Availability: Items available for loan: UVAS Library [Call number: 2190,T] (1).



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