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1. Prevalence Of Soil Borne Helmithiasis Of Zoonotic Importance In Dogs

by Muhammad Tayyab Farooqi | Prf.Dr. Azhar Maqbool | Dr.Irshad | Dr.Kamran Ashraf | Faculty of Veterinary Sciences.

Material type: book Book; Format: print Publisher: 2008Dissertation note: To record the prevalence of soil borne helminthiasis of zoonotic importance in dogs' one hundred faecal samples were obtained from pet dogs, fifty from pups of age less than six months and fifty from adult dogs. Four hundred soil samples along with grass were also be collected from different dog kennels, parks and playgrounds, backyards and streets of Lahore city, where soil and grass is contaminated with the faeces of stray dogs. After collection, the faecal samples were preserved in polythene bags and kept in refrigerator or preserved in 10 % formalin to avoid any other contamination of the samples. Then the samples were brought to the Diagnostic Laboratory, Department of Parasitology University of Veterinary & Animals Sciences, Lahore. Microscopic examination was performed by using different parasitological techniques like Direct Smear Method, Floatation Method and Sedimentation Method for the detection of the parasite ova. Grass Samples were examined through Sedimentation-Flotation Method. The results were analyzed by using chi2 test to check the significance difference of the ova in soil samples of different areas. the result showed that out of 400 soil and grass samples 33 were positive for Ancylostoma caninum, 37 were positive for Toxocara canis, 29 were positive for Dipylidium caninum and 19 were positive for Echinococcus granulosis and out of 100 faecal samples taken from pups and adult dogs, 10 were positive for Ancylo stoma caninum, 13 were positive for Toxocara canis, 8 were positive for Diplyidium caninum and 6 were positive for Echinococcus granulosis. The results suggested that better deworming stragies shold be adopted so that the health of the dogs can be improved and this will also reduce the reisk of zoonotic infectin in humans, entry of the stray dogs in public areas should be restricted to avoid the risk of zoonotic diseases. Availability: Items available for loan: UVAS Library [Call number: 1019,T] (1).

2. Detection And Taxonomy Of Dust Mites In The City Of Lahore With Special Reference To Allergic Manifestation

by Muhammad Tanveer Khan | Dr.Kamran Ashraf | Dr.Irshad | Mr.Awais Anees | Faculty of Veterinary Sciences.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2009Dissertation note: The most important allergy causing mites found worldwide in houses are the house dust mite's i.e. D. farinae & D. pteronyssinus and most houses contain multiple species. The geographical distributions of mite species are vary between houses within a geographical region and among different areas. Therefore it is need to know the exact prevalence of species in a particular area when diagnostic testing is performing and prescribing immunotherapy. Among environmental factors, temperature and humidity play a key role for the survival as well as prevalence of house dust mites. The most favorite place for the better survival of house dust mites is houses belong to the humid geographical areas. In this study, 1280 dust samples from carpet, bedroom mattress, sofa and floor of total 64 houses from posh area and old city area from May to August, 2007, were collected and two examination procedures were selected (Acarex Strips Test and Direct Microscopic Examination). 320 dust samples (160 posh area and 160 old city area) were detected with Acarex Strips Test and remaining dust samples were examined by using the direct microscopy. In the present study, the overall occurrence of house dust mites in dust samples of carpet, bedroom mattress, sofa and floor was recorded as 22.5% (9), 22.5% (9), 20% (8) and 10% (4) respectively from posh area by using the Acarex strips test from May to August, 2007, at Lahore. Similarly from old city area, the overall occurrence of dust mites from house dust samples of carpet, bedroom mattress, sofa and floor was reported as 27.5% (11), 30% (12), 22.5% (9) and 15% (6) respectively. Direct microscopy was performed for remaining 960 (240 carpet, 240 bedroom mattress, 240 sofa and 240 floor) dust samples to examine the occurrence and taxonomy of house dust mites. Among 26.3% (63) positive dust samples of carpet 63.5% (40) were found positive for D. farinae and 36.5% (23) for D. pteronyssinus. Total 28.8% (69) bedroom mattress dust samples were found positive for house dust mites but 74.9% (51) and 26% (18) dust samples were containing D. farinae and D. pteronyssinus species respectively. Among 17% (41) positive dust samples of sofa, 61% (25) were containing 13. farinae and 39% (16) D. pteronyssinus. Out of 11.7% (28) positive dust samples of floor for house dust mites, 68% (19) were examined positive for D. farinae and 32% (9) for D. pteronyssinus. The high occurrence of house dust mites were seen during May, June, July, and August 2007. During May, 2007, there was not so highly occurrence of house dust mites observed as compare to other three months (June, July and August, 2007). From late June rainy season (monsoon) was started and high moisture remains in the environment to late August. These environmental conditions have direct impact on the population of house dust mites. From previous study, it was suggested that two species (D. farinae and D. pteronyssinus) occur in the area of Lahore, Pakistan. The highly prevalent specie in the house dust samples of carpet, bedroom mattress, sofa and floor was reported D. farinae as compare to D. pteronyssinus. Availability: Items available for loan: UVAS Library [Call number: 1045,T] (1).



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