51.
Taxonomical Studies Of Endo Parasites & Their Sequence Of Prevalence At Different Ages Of Layers
by Anwar ul Haq | Dr. Hajid Ahmad Hashmi | Dr. Mubasher Saeed Mian | Dr. Sh | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1994Dissertation note: In the present study the total infection of gastro-intestinal parasites were studied in commercial layers, maintained at various poultry farms located in and around Lahore. About 930 faecal samples were collected from those birds which were dewormed at least one month before. Birds were divided into six age groups starting from Week-i to 48.
Out of 930 faecal samples, 689 (74%) were positive for parasitic infection and 241 (26%) were negative. Ascariclia g.1ij was found in 432 (46.4%) faecal samples, Heterakis gallinarum 326 (35%) and Coccidiosis in 237 (25.4%) of the total faecal samples examined.
The age wise incidence of parasites in group A (1-8 wks) was 59.3%, in group B (9-16 wks) 66.04%, iii group C (17-24 wks) 74.1%, in group D (25-32 wks) 74.8%, in group E (33-40 wks) 88.3% and in group F (41-48 wks) 81.2% i.e. ascending order of infection. In case of seasonal prevalence of infection, the occurrence of Nematodes in June was 41.25%, in July 40.25%, in August 45.75% and in September 38.80%. The incidence of Coccidiosis in June was 27.7%, in July 24.8%, in August 16.1% and in September 28.5%. The results were recorded on the basis of E.P.G. (Egg Per Gram) of nematodes and O.P.G. (Oocyst Per Gram) of Eimeria species. In general low body weight gain and low Egg production (30-40%) in the parasitised birds as compared with non-parasitised birds were noted. Statistical analysis revealed non-significant difference between prevalence of different species of parasites in the birds.
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52.
A Study On The Chemotherapy And Taxonomy Of Mange Mites In Ovine And Its Effect On Different Blood Parameters
by Rafique Rana, M | Dr. Muhammad Afzal | Dr. Mubasher Saeed Mian | Prof. Dr. SAghir | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1993Dissertation note: A study was carried out to find out the taxonomy, chemotherapy of mange mites in sheep and its effect on different blood parameters. A total of 30 flocks comprising of fifteen hundred sheep of different age and sexes were examined in the city and villages around Lahore. Out of above mentioned animals 200 suspected sheep were thoroughly examined and 30 (15%) were found positive for mange mites infestation. Species of three genera namely Sarcoptes, Psoroptes and Chorioptes were found infesting these animals.
The average number of mites recovered from the species of each genera were recorded as 851, 825 and 815 for Psoroptes, Sarcoptes and Chorioptes respectively. For the identification of different species of mange mites microscopic examination was carried out and mites were first seen under low power and then high power of magnification. The slides were mounted with mites and then examined to study their morphological characteristics.
The three genera identified were Sarcoptes, Psoroptes and Chorioptes in sheep.
Chemotherapeutic trials were carried out in suspected sheep by using two concentrations of neguvon solution i.e. 0.10% and 0.15% at ten days intervals. For this purpose sheep were divided into three groups. Group A was treated with 0.10% solution of neguvon, group B with 0.15% solution of neguvon and group C was kept as untreated control group. The data obtained on reduction of mites due to acaricide application were subjected to statistical analysis by applying completely randomized design and results were recorded. It was noticed that 0.15% neguvon was highly effective against mange mites infestation when it was replied twice after ten days interval.
The effect of mange mites on different blood parameters was also recorded. For this purpose sheep were divided into three groups. Group I comprised of clinically infested sheep, group II subclinically infested sheep and group C included healthy sheep which was kept as control for the comparison. The effect of mange mites on different blood parameters of two infested groups of sheep i.e. I and II was recorded before and after two applications of 0.10% and 0.15% neguvon solution at ten days interval and results were compared with group C for comparison.
The findings of this study indicated that the infestation of mange mites is a quite common problem in sheep population of this area. The sheep suffering from mange mites exhibit poor feed intake, severe irritation, scratching, bleeding, loss of wool, low body growth, reduced production and damaged skin. Such infested sheep are also susceptible to various viral and bacterial diseases which makes sheep production uneconomical and put extra burden on the sheep breeders.
Keeping all this in view suitable and prompt measures should be taken for early diagnosis and treatment of the infested animals. This will result not only for the control of the disease, but also help to improve the economical condition of the owners. It will also increase the gross national product of Pakistan.
Availability: Items available for loan: UVAS Library [Call number: 0386,T] (1).
53.
A Study On The Taxonomy Of Sheep Cestodes And The Efficacy Of Albendazole And Miclosamide Against Natural Infections
by Ashraf, M | Dr. Asif Rabbani | Dr. Mubashar Saeed Mian | Dr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1994Dissertation note: Pakistan has a large population of live stock, which plays an important role in the economy of the country. Parasitism adversally effects the growth and production of livestock. Great economic losses have been attributed to the sheep population in our country due to the parasitic infections including cestodes. A study of cestodal infections with taxonomy of the prevalent cestoctes species affecting sheep at different age groups in and around Lahore was conducted. The taxonomical study was conducted in the month of August, September and October, 1993. For this purpose 200 guts (one hurtdered each from below six month and above six month of sheep) were collected from Lahore Abattoir. Parasites were identitied under the microscope by preparing permanent mounts.
The over all incidence of cestodes infection in sheep was 65.5%.
The species of various genera of cestodes observed during the present
study were:
Moniezia pansa (64%), Moniezia benedeni (60%), Avitellina cjj[iur1ctta
(49.50%), Avitellina lahorea (40%), Avitellina sudanea (30%), Stilesia vittata
(30%), Stilesia globiounctata (50%) and Thvsanosoma actinioides (6%).
Monthwise prevalence of cestodes infection was also carried out and it was observed that the highest infection rate was (76%) during the month of August and (66.25%) during the month of September. While the lowest incidence was recorded as (57.14%) during the month of october, 1993.
The taxonomy of the prevalent cestode species was carried out. The effect of age of the animals on the intensity of cestodal infection was also studied. There was a significant difference in the prevalence of cestode infections among the two groups.
The present work was also aimed to verify the efficacy of two commonly available anthelmintics namely albendazole (valbazen) and niclosarnide (mansonil), in naturally infected stceep with Gastrointestinal cestocJes. Fifty sheep positive for cestods infections were randomly divided into two groups A and B, each group comprising of 25 animals. Group. A was given albendazole (valbazen) at a dose rate of 2.5 mg/kg body weight orally while the group B was administered niclosamide (mansonil) at a dose rate of 100 mg/kg body weight orally. The efficacy of both the drugs was evaluated on the bases of reduction of segments and number of eggs per gram of faeces after medication. The egg counts were made on zero, 3rd and 21st day. Both the drugs revealed a progressive decrease in the faccal egg/segment counts. The efficacy of albenclazole on 3rd and 21st day was 96.02 and 99.23% repectively while the efficacy of niclosamide (mansonil) was 100% on 3rd and 21st day.
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54.
The Host Reaction To Experimentally Induced Eimeria Infections In Broilers Chicks
by Ata ur Rehman Awan | Dr. Haji Ahmad Hashmi | Dr. Mohammad | Dr. Mubasher Saeed Mian | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1994Dissertation note: The experiment was designed to study the host reaction to the experimentally induced Eimeria species in broiler chicks. For this purpose two hundred and twenty, one-day-old chicks were reared. At the age of day 3, they were divided randomly in four groups each comprising of 55 birds. They were named as A, B, C and D. The group A was kept as un-infected control, while group B, C and D, at the age of day 3, received a primary dose of 1000 virulent sporulated oocysts, through oral, intraperitoneal and subcutaneous routes respectively. The challenge doses of 50,000 virulent sporulated oocysts each were adminigtered to the members of each group B, C and D on days 13, 28 and 43 of age.
The OPG counts in group A remained nil throughout the experiment the maximum OPG counts 65,500 were recorded in group B by the day 21. On the same day OPG counts of group C and D were also on the peak found to be 40,500 and 54,500 respectively. Three mortalities occurred in group B, 4 in C and 6 in group D. Similarly the maximum weight was attained by the members of group A (Control) i.e. 1750 gm, secondly by group B (oral) i.e. 1530 gm, thirdly group D (S/C) i.e. 1410 gm and fourthly group C (I/P) i.e. 1280gm. The difference in B was of 220gm, in C 470 gm, and in D 340 gm lesser as compared to group A.
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55.
A Study On The Prevalence & Taxonomy Of Biting & Sucking Lice Infestation In Sheep With Their Effect On Different Blood Parameters
by Jamal Abdul Nasir | Dr. Muhammad Afzal | Dr. Mubasher Saeed Mian | Dr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1995Dissertation note: The study was planned to determine the prevalence and taxonomy of biting and sucking lice infestation in sheep with their effect on various blood parameters. For this purpose specimens were collected from Outdoor Hospital College of Veterinary Sciences, Lahore and various private clinics in the city.
The collected specimens were preserved, stained and ide.ntified after preparing permanent mounts under the sterioscopic microscope in the laboratory. Two species of lice i.e. Damalinia (biting lice) and Liiioqnathus pedalis (sucking lice) were identified having a percentage of 10.50 and 1.0 respectively. The infestation recorded was highest during spring and autumn and lowest during the summer.
The taxonomy of the identified species were carried out by using morphological characters (Chandler, 1961 and Soulsby, 1982).
Haernatological examinations were made to compare the blood picture of healthy and infested sheep. For this purpose 20 blood samples each from healthy and infested sheep were examined. Five ml of blood was collected from the jugular vien of the sheep, with the help of disposable syringes, in a test tube having few drops of 0.1 % EDTA.
The blood parameters studied were: Haemoglobin level, Total Erythrocyte Count, Total Leukocyte Count, Packed Cell volume and differential leukocytic count. The results of haematological examination showed a significant decrease in haemoglobin, total erythrocyte count and packed cell volume, whereas a significant rise in leukocytic count and D.L.C. of infested sheep as compared to healthy sheep was observed.
The results of the present study will help in planning better control measures in order to minimize lice infestation and also aid in the eradication of various lice born diseases in sheep.
Availability: Items available for loan: UVAS Library [Call number: 0407,T] (1).
56.
A Comparative Study Of Helminth And Haemoparasites Of Domestic And Wild Pigeons
by Asma Hussain | Dr. Mubashir Saeed Mian | Dr. Asif Rabbani | Dr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1995Dissertation note: There is an increasing interest in pigeons and other game and ornamental birds. These birds are generally kept either free roaming or confined in outdoor pens and hence are vulnerable to various parasitic infectious, which greatly effects the productivity of these birds.
The meat production of the pigeons can be improved by controlling disease problems particularly helrninths and blood protozoan infections, so that in near future they may contribute towards narrowing down the animal protein supply gap by substituting poultry meat with squabs.
The present work was planned for the comparative study of helminths and heemoparasites of domestic and wild pigeons. For this purpose 300 each of guts and blood smears were studied. The overall incidence of gastrointestinal helminths and blood protozoans was 77.33% and 31.99% respectively, while 36% of birds had mixed infection. The incidence of gastro-intestinal helminths and blood protozoa in wild pigeons was 89.33 and 20.66 percent respectively and in domestic pigeons it was 65.33% and 11.33% respectively. Whereas mixed infections were 22% and 14% respectively.
The following species of helmitiths and blood protozoa were recorded and identified.
1. Raillietina tetragona
2. Raillietiiia cesticillus
3. Choanolaenia infundibulum
4. Ascaridia colurnbae and
5. Cap illaria obgnata
The two species of blood protozoa recovered were:
1. Aegypanella pullorum and
2. Haeiçotuscumbae
Among the helrninths recorded, cestodes were found predominating as compared with nernatodes in both wild and domestic pigeons. Raillietina cesticillus was the most common cestode species in both Wild and domestic pigeons i.e. 51 and 33 percent respectively while Asci colurnbae predominated the nernatode species i.e. 18 and 15 percent respectively. The Incidence was higher in wild pigeons.
Aegyptianella pullorum was more common blood protozoan i.e. 22 and 11 percent respectively in wild and domestic pigeons. The incidence of Haemoproteus clumbae was 9 and 11 percent respectively.
Availability: Items available for loan: UVAS Library [Call number: 0410,T] (1).
57.
Study Of Gastro-Intestinal Helminths And Taxonomy Of Species Of The Genus Paramphistomum In Camels
by Saeed A. Khan | Dr. Muhammad Afzal | Dr. Mubasher Saeed Mian | Dr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1994Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0411,T] (1).
58.
The Effects Of Piroplasmosis On Hematology In Equines
by Salva | Dr. Mubasher Saeed Mian | Dr. Asif Rabbani | Dr. Shakeel | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1996Dissertation note: Piroplasmosis is a serious disease of the domestic animals including horses. It is caused by species of the genus Babesia i.e. . egui and B. caballi resulting in death due to excessive loss of blood. The present study was conducted to find out the effect of natural Piroplasmosis on various blood parameters, viz, total erythrocytes count (TEC), hemoglobin estimation (Jib), packed cell volume (PCV), total leukocytes count (TLC) and differential leukocytes count (DLC) in horses.
A total of forty horses (Group A including 20 uninfected horses and Group B included 20 horses showing clinical symptoms of the disease and confirmed positive for piroplasmosis through blood examination were used in the study and found to cause the infection although . caballi infection was more predominant.
Heamatological examination revealed a significant decrease (P<0.05) in the erythrocyte count, hemoglobin level and packed cell volume of the infected horses.
Normocytic and hypochromic type of anemia was observed. Total leukocyte count was also decreased. A decrease in the number of rieutrophils, eosinophils, basophils, monocytes and lymphocytes was also observed in the infected horses.
Out of the 20 infected horses, 4 had B. equi and sixteen had B. caballi infection indicating that . caballi was more prevalent.
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59.
A Study Of Gastro Intestinal Parasitism And Haematological Disturbances Associated With Single Or Multiple Infection In Sheep
by Hafeez, M | Dr. Asif Rabbani | Dr. Mubashar Saeed Mian | Dr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1996Dissertation note: Nature has blessed Pakistan with a large population of livestock which play an important role in the economy of the country. Parasitism adversely effects the growth and production of livestock. Great economic losses have been attributed to the sheep population in our country due to parasitic infections including gastro-intestinal parasitism. A study of gastro-intestinal parasitism with isolation and identification of the parasite species affecting sheep at different age groups in and around Lahore was conducted in the months of July, August, September and October, 1994.
For this purpose 200 G.I. tracts of sheep (One hundred each from below six months and above six months of age) were collected from Lahore Metropolitan Corporation, Abattoir.
The overall incidence of gastro-itestinal parasitism in sheep was found to be 68.5%. Age group wise incidence was found to be 71% and 66% in sheep below and above six months of age respectively. Classwise overall incidence was found to be Sporozoan (51%), Trematodes (21%), Cestodes (62%) and Nematodes (68%).
Age group wise incidence of Sporozoan, Trematodes, Cestodes and Nematodes was found to be 60% and 42%, 12% and 30%, 71% and 53%, 73% and 63% in sheep below and above six months of age.
Month-wise prevalence of gastro-intestinal parasitism was also carried out which was higher in the month of August and lowest in the month of October. Nineteen different species of Parasites were recorded which are detailed as under:
1. Eimeria arloingi
2. Eimeria ninakohlvakimovae
3. Eimerla parva
4. Elmerla intricata
5. Eimeria faurei
6. Paramphistomum cervi
7. Cotylophoron cotvlophorum
8. Moniezia. expansa
9. Moniezia benedeni
10. Avitellina centripunctata
11. Haemonchus contortus
12. Oesophagostomum colunThianum
13. Oesophagostonim venulosum
14. Trichuri
15. Ostertagia circumcincta
16. Ostertagia ostertagi
17. Chabertia ovina
18. Trichostrongvlus colubriformis
19. Nematodirus spathiger
The isolation and identification of various collected parasites was carried out. The relationship between the age of sheep and the occurrence of gestro-Intestinal parasitism was also studied. There was a Non Significant difference in the prevalence of gastro-intestinal parasites among the two groups.
The present project was also aimed to observe the effect of single or multiple parasitism on certain haematological parameters like, estimation of haemoglobin contents, total erythrocytic count, total leukocytic count, erythrocyte sedimentation Rate, packed cell volume and differential leukocytic count.
These heamatological studies were carried out on control (parasitic free) and parasitised sheep of below and above six months of age.
The findings of haematological studies showed that there was decrease in haemoglobin contents. total erythrocytic count, packed cell volume, total leukocytic count and increase in erythrocyte sedimentation rate in both sheep below and above six months of age under the effect of single or multiple gastrointestinal parasitism. The values of differential leukocytic count were variable in both age groups.
Most of the haematological disturbances on various blood parameters in both age group of sheep were proved to be statistically significant.
From the results of the presents study, it has been concluded that the incidence of gastro-intestinal parasitism in sheep below and above six months of age is on the higher side which warrants that certain essential preventive measures must be adopted to safeguard our valuable livestockfrom these serious parasitic infestations. It is also evident from the results that gastro-intestinal parasitism adversely affects the blood components in sheep which may lead to anaemia, loss of growth and loss of production.
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60.
Comparative Efficacy Of Anthelmintics Against Gastro Intestinal Parasites Of Buffalo Calves And Their Effects On Blood Parameters
by Tariq Humayun | Dr. Mubasher Saeed Mian | Dr. Haji Ahmad Hashmi | Dr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1995Dissertation note: A total of 90 (60%) out of 150 fecal samples from buffalo calves of different age groups were found positive for gastrointestinal parasites. Coprological examination revealed Toxocara vitulorurn (42.22%) as single infection and 31.11% as mixed infection. Strongy.Zoides papillosus as 21.11% as single infection arid 20% mixed infection. Nematodirus spp. 1.11% as single. Haemonchus sp., 2.22% as single and 1.11% mixed infection. Honiezia sp. 3% as single and Eimeria spp., 21% as single and 13.33% as mixed infection. Intensity of infection of gastro-intestinal parasites in buffalo calves was determined as 41.1% of calves upto 500 eggs per gm of faeces, in 22.22% calves from 501 to 1000 epg, in 16.66% calves from 1001 to 1500 epg and in 20% calves greater than 1500 epg of faeces.
Comparative percentages of means of EPG counts of infected control subgroups of groups A,B and C showed increasing trend of EPG counts by day 14. However subgroup A3, showed 'I3.l% reduction, subgroup B3 showed 61.15% reduction and C) showed 72.29% reduction in epg counts on day 3 after medication with oxfendazole. Similarly on day 7 of medication, the reduction percentage were, 95.52, 89.93 and 96.38 in respective subgroups. Albendazole in subgroups A4, 84 and C4 showed reduction on day 3, by 67.08, 64.89 and 78.57 and 95.24 respectively. On day 14, both the anthelmintics showed 100% etticacy No significant change was noted in RBC, UBC counts Hb gm/mi and PCV%, however, Eosinophils and lymphocyte counts depicted siynificant increase in the infected subgroups.
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61.
A Study Of Gastro Intestinal Helminthiasis And The Effects Of Natural Infection On Various Blood Parameters In The Buffaloes
by Ishtiaq Ahmad | Dr. Asif Rabbani | Dr. Mubasher Saeed Mian | Dr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1995Dissertation note: A total of 76 buffaloes brought to the Lahore Metropolitan Slaughter house were studied for helminthiasis. Out of which 50 (65.79%) were found positive.
The helminthiasis positive guts were examined for the presence of helminths and the following species were identified Haemonchus contortus (11.84%), Haemonchus placei (10.53%), Ostertagia ostertagi (7.89%), Trichostrongylus axei (3.95%), Mecistocirrus digitatus (2.63%), Trichostrongylus colubriformis (7.89%), Cooperia oncophora (9.21%), Cooperia punctata (5.26%), Cooperia pectinata (6.58%) , Nematodirus helvetianus (2.63%), Bunostomum phiebotomum (2.63%), Strongyloides papillosus (2.63%), Oesophagostornum radiatum (3.95%), Trichuris globulosa (1.32%), Paramphistomum cervi (3.95%), Paramphistomum microbothrium (2.63%) and Monezia benedeni (2.63%)
The effect of helminthiasis on blood parameters like, total erythrocytic count, total leukocytic count, packed cell volume, erythrocytic sedimentation rate, haemoglobin concentration was investigated and the values recorded were 5.840±0.110x106 Cimm, 5.447±0.074x103 Cumm, 32,858±0.230%, 38.004±0.217 mm/hr. and 10.368±0.096 gm/lOOml respectively. The differential leucocytic count values for neutrophils, lymphocytes, eosinophils, monocytes and basophils were
43.16±0.531%, 43.82±0.718%, 6.40±0.187%, 5.620±0.241% and 1.040±0.124% respectively.
Similarly the helminth free (control> animals studied for their total erythrocytic count, total leukocytic count, packed cell volume, erythrocytic sedimentation rate, haemoglobin concentration, gave their averages as 7.382±0.068x106 Cmm, 6.321±0.084x103 Cimm, 38.927±0.286%, 28.213±0.148 mm/hr. and 11.892±0.286 gm/lOOml respectively. The differential leukocytic count values for neutrophils, lymphocytes, eosinophils, monocytes and basophils were
29.15±0.365%, 56.0±0.513%, 8.12410.28%, 5. 852410 .264% and 0.90±0.191% respectively.
The average values for helminth infected and helminth free animals were analysed statistically. The infected animals had a significantly decreased total erythrocytic count, total leukocytic count, packed cell volume, haemoglobin concentration, lymphocytes and eosinophils with increased erythrocytic sedimentation rate and neutrophils, while no significant difference was seen in monocytes and basophils.
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62.
Effects Of Theileriasis On Various Blood Parameters And Total Serum Protein In Exotic And Cross Bred Cattle Present In And Around Lahore
by Arshad Mahmood, Ch | Dr. Muhammad Afzal | Dr. M. Sarwar | Dr. Muhasher Saeed Mian | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1994Dissertation note: A study was conducted on Theileriasis in rfleilel.ia species, as well as haematological values were conducted by using 28 crossbred and 22 exotic cattles. Theileria annulata was the only species identified in the erythrocytes of infected cattle. Clinically, signs of panting, temperature ranging from 103.8 to 105.8°F, swelling of superficial lymph nodes, salivation and decrease in milk production were observed in infected animals.
There were significant differences in haematological values in infected cattle as compared with non-infected ones. There was decrease in total erythrocytic count, haemoglobin concentration, packed cell volume, Total leukocytic count, serum protein and neutrophils. Whereas lymphocytes showed an increase in the infected cattle.
Regarding the comparison of crossbred and exotic cattle, there was statistically significant difference in both the means of total erythrocyte count, PCV, Haemoglobin, Serum Protein, lymphocytes and neutrophils. But no significant difference was seen between means of eosinophils, Basophils and monocytes of the exotics as well as the crossbred cattle.
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63.
The Effects Of Induced Coccidiosis On Growth And Blood Parameters In Commercial Quails (Coturnix Coturnix
by Ashraf, M | Dr. Asif Rabbani | Dr. Mubashar Saeed | Dr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1994Dissertation note: The study was designed to see the effects of experimentally induced coccidiosis op growth and blood parameters in commercial quails (coturnix coturnix japonica). For this purpose 200 day old quail chicks were obtained from a local hatchery and raised under standard, controlled coccidia free conditions. A commercial coccidiostat free feed was provided. At the age of 21 days, birds were divided into 2 groups A and B each comprising 100 birds.
Birds of group 'A' were kept as non infected control and birds of group 'B' were infected with I ml of coccidial inoculum having 50,000 sporulated oocysts given directly into crop. After the infection, the OPG of the both groups was recorded. Weekly weight gain/bird of both A and B group was recorded. Meanwhile the morbidity, mortality, clinical finding and post-mortem finding were also observed. At the end of experimental period of 42 day, 20 birds from each group were slaughtered and blood sample were collected for haematology.
The weight of birds in group 'B' was significantly lower than that of group 'A' birds. The morbidity and mortality rate in group B were 100% and 38% respectively.
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64.
A Study On Gastro Intestinal Nematodes Of Camels Slaughtered At Metropolitan Corporation Abattoir Lahore With Taxonomy Of The Isolated Species
by Abbas Ali, Syed | Dr. Asif Rabbani | Dr. Mubasher Saeed Mian | Dr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1993Dissertation note: The present study was carried out to determine the incidence of gastro-intestinal nematodes of camels, with the taxonomy of the isolated species. Sixty guts were examined rendomly at the rate of 15 guts per month from August to November, 1993. The adult parasites were collected, fixed and preserved. The whole mounts were prepared. The faecal samples were examined and the EPG was estimated using McMaster egg counting technique. The taxonomy of the isolated species was determined by examing the whole mounts, according to the morphological characters given in the keys. The five species of nematodes representing different genera were isloated and identifed as Haenionchus ion gistipes, Izlaemonchus contortus, C'ainelostrongy!us mentulatus, Neniatodirus spathiger and Trichuris giobulosa.
The overall incidence recorded from camels during the present study was 65 percent. The morphological characters were studied by preparing permanent mounts and were described.
The findings of the present study will help in planning programme for the control of parasitic disease, especially caused by the above mentioned species of nematodes of camels in Pakistan, which will contribute to the development of animal industry by providing much effective coverage for the devastating effects of the parasites and will ultimately enchance the production of milk, meat and hides.
Availability: Items available for loan: UVAS Library [Call number: 0463,T] (1).
65.
A Study On The Epidemiological Aspects Of Fascioliasis In Buffaloes In Lahore District
by Rabbia Sahar | Dr. Asif Rabbani | Dr. Haji Ahmed Hashmi | Dr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1996Dissertation note: The study was designed to find the prevalence and intensity of liver fluke infection in buffaloes in Lahore District. The study period was from April 1995 to July 1995. The data was based on:
Data collected from four Veterinary Hospitals in Lahore District namely, Herbenspura, R.A. Bazar, Shamkey Bhattian and Rukh Chandra indicated that during the study period from 1 April, 1995 to 31 July, 1995, a total of 2184 buffaloes were treated for different diseases in these hospitals. Based on history, clinical symptoms and response to treatment 229 (10.48%) buffaloes were positive for fascioliasis.
Information collected from Municipal Corporation, Lahore abattoirs regarding economical losses revealed that one hundred eight million rupees were lost annually due to damage and condemnation of liver fluke infected livers.
Two hundred faecal samples collected directly from the rectum of buffaloes from various localities at Lahore were examined microscopically by fresh smear and sedimentation methods, which revealed that 75 (37.5%) buffaloes were suffering from fascioliasis.
In order to determine the intensity of Fascioliasis in infected animals, quantitative faecal examination was done (and it ranged from 32.6 to 45.6 EPG indicating that the disease is likely to be pathogenic).
Overall liver fluke infection was recorded by examining forty livers along with their bile ducts collected from slaughtered buffaloes and it was found that 16 (40%) had liver fluke infection.
Identification of live flukes from the infected flukes revealed that 8 (50%) had mixed infection of Fasciola hepatica and Fasciola gigantica, 5 (31.25%) had only Fasciola gigantica infection while 3 (18.75%) had only Fasciola hepatica infection.
It was concluded from the above studies that Fascioliasis in buffaloes in Lahore district is quite prevalent, and it needs to adopt appropriate measures to control the infection.
Availability: Items available for loan: UVAS Library [Call number: 0485,T] (1).
66.
Taxonomical Study Of Treatment Infections And Their Effect On Blood Picture In Sheep And Goats
by Sulman Hameed | Dr. Asif Rabbani | Dr. Haji Ahmad Hashmi | Dr. M. Sarwar | Faculty of Veterinary Sciences.
Material type: ; Format:
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; Literary form:
not fiction
Publisher: 1996Dissertation note: In the present study two hundred sheep and two hundred goats both above one year of age and below one year age were examined after their slaughtering at abattoirs of Lahore for the presence of Gastrointestinal trernatodes during the month of May to August, 1995.
Monthwise prevalence of p. Cervi, f... hepatica a6% D. dendriticum and D. dendriticurn during the month of May to august, 1995, was as under:
May 1995 15% cervi, 20% f hepatica 6% D. dendriticum
June // 19% P.cervi 27% f. hepatica 8% D. dendriticum
July // 33% P.cervi 40% hepatica 16% D. dendriticum
August // 28%P.cervi 32% f hepatica 13% D. dendriticurn
It was observed that the infection increased gradually and reached at peak in the month of July and then declined. An overall incidence of 64.25% of trematodes was recorded in both the species. The parasitic incidence was as . cervi 23.75%, .J hepatica (29.75%) and .. dendriticum (1 0.75). So the rate of infection was on the peak in the month of July in P.cervi, E. hepatica and . dendriticurn as shown in Figure 1. The hernatological values of sheep and goats infected with trernatodes were :total Erythrocytic count 1 2.01 3 ± 0.096 million/cubic microliter, Haemoglobin level 8.037±0.160 g/l00rnl, Packed cell volume 30.10 ± 0.326%, IErythrocytic sedimentation rate 2.71 ± 0.242 mrn/24 hours and Total Erythrocytic count 10.973 ± 0.094 million/ microliter, Haemoglobin 7.637±0.128 g/1 OOml, Packed cell volume 28.15 ± 0.316% and Erythrocyte sedimentation rate 1.94±0.246 rnm/24 hour respectively for sheep and goats. It was observed that the infection with trernatode had an adverse effect on total erylhrocyte count, haemoglobin level and packed cell volume.
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67.
Taxonomuy And Prevalence Of Eimeria Species Infecting Young Buffalo And Cattle Calves And Their Effect On Various
by Afzal, M | Dr. Muhammad Afzal | Dr. Haji Ahmad Hashmi | Dr. Shakil | Faculty of Veterinary Sciences.
Material type: ; Format:
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; Nature of contents: ; Literary form: Publisher: 1996Dissertation note: This study was contemplated to evaluate some aspects of prevalent species including taxonomy of Eimeria infection& in young cattle and buffalo calves. In addition the effects of Eimeria infections on various haematological parameters i.e., Haemoglobin, PCV, RBC count, ESR and Differential leucocyte count were also studied. For this purpose the animals (Buffalo and Cattle calves) were divided into two groups. Firstly those having diarrhoea and secondly those which were apparently healthy. The buffalo and cattle calves were divided into four groups (A, B, C and D) on the basis of the age in days under the maximum age of 120 days. A group ranged from 1-30 days, B ranged 31- 60 days, C ranged 61-90 and D ranged 91-120 days. The haematological observations were recorded from both the groups formulated upon the basis of age as A, B, C and D in both cattle and buffalo calves and findings compared with the control groups A, B, C and D having the same age range.
Results revealed that out of 816 animals (442 cattle and 374 buffalo calves), 167 (84 cattle and 83 buffalo calves) were found to be positive (20.46%) for Eimeria species infection. Whereas 62 out of 250 (24.80%) suffering from diarrhoea and 22 out of 192 (11%) apparently healthy cattle calves showed coccidial infections. For buffaloes 63 out of 220 cases (29%) were positive from animals showing diarrhoea and 20 out of 154 (13%) were found positive for coccidiosis from apparently healthy group. Four species of Eimeria namely, Eimeria zuernii, Elmerip bovis, Eimeria cylindrica and Eimeria ellipsoidalis were found in the above mentioned ca1ves
E. zuernhi was the most prevalent species found (48%) followed by . bovis (27%), Eimeria cvlindrica (16%) and Eimeria ellipsoidalis (10%) respectively. OPG count carried out ranged from 2500-70,000 oocyst per gram of faeces.
Haematological observations showed anaemia, which was a feature of the diarrhoeic calves and not found in apparently healthy animals although some of them had been harbouring Eimeria infection. The PCV decreased proportionately with haemoglobin whereas erythrocyte count also showed decrease in positive cases. On the other hand ESR of the above referred calves increased. There was neutrophilia in the infected animals versus control animals and lymphocytes decreased in infected animals. There was no significant change in eosinophil and monocyte counts.
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68.
Taxonomical Studies Of Prevalent Species Of Eimeria In Broiler Chicks And Comparison Of Immucox (Imported Vaccine) Versus Locally Prepared Vaccine Against Coccidiosi
by Sodaghar Ali Shaker | Dr. Muhammad Afzal | Dr. Haji Ahmad Hashmi | Dr. Shakil | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1997Dissertation note: The study was designed to identify different species of the genus Eimeria in broiler chicks occuring in poultry farms situated in and around Lahore.
For this purpose one thousand faecal samples were collected from different broiler farms out of which 145 (14.5%) were positive for coccidiosis. Two hundred broiler carcases, suspected to he suffering from coccidiosis were obtained from different diagnostic Laboratories at Lahore. One hundred and ninety out of 200, (95%) were found positive for coccidiosis.
Identification of the species of the genus Eimeria was done and seven species were identified i.e, E tenella 119 out of 190 (62.63%), J. brunetti 27 (14.21%), E. necatrix 13 (6.84%), . acervulina 19 (10%), . maxima 4 (2.10%), . mitis 3 (1.58%) and . praecpx 5 (2.63%).
The second aim of the present study was to compare the immunogenic properties of locally prepared (atlenuated) and imported (live) vaccine (Immucox). For this purpose one hundred and fifty five day old broiler chicks were reared under controlled conditions. They were equally divided into five groups i.e. A, B, C, D and E. Group A was kept as uninfected control. Group B acted as infected control. Group B, C, D and E were infected with 50,000 sporulated Oocysts at day 15, 25, 35 and 45 of age. Group C acted as infected and treated control alongwith 5 days treatment with amprolium, after three days of each primary and challenge doses. Group D received 1000 sporulated formalin treated oocysts orally, at the age of day 3 and day 10. The members of group E received orally imported vaccine (Immucox) at the age of day 5.
Immunogenicity was measured by the passage of oocysts per gram of faeces (OPG) and by comparative weight gains amongst different groups. It was noticed that no coccidiosis could be established in groups A, C, D and E. The OPG counts remained nil in groups A and C throughout the experimental period. Groups D and E showed moderate counts and Group B showed high counts of OPG. The OPG counts gradually decreased to 35,000, 1200 and 1150 in groups B, D and E, respectively at the end of the experiment.
Average weight gain records showed 430, 150, 332 and 270 gms greater in A, C, D and E groups, respectively at the end of the experiment, as compared to group B.
FCR of group A, B, C, D and E was 2.82, 3.48, 3.11, 2.76 and 2.93, respectively at the end of the experiment. Group D was the best and group B was the worst. Group D depicted full protection and remained second to the uninfected control group (A) in respect of weight gains. Group E also showed complete protection and remained third to the uninfected control group A in respect of weight gains. Inspite of complete protection in group C due to amprolium treatment, the weight gains remained lower than groups D and E. There was no development of exogenous species of Elmeria due to immunization with immucox.
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69.
Studies On The Incidence Of Gastro Intestinal Helminths And Their Economic Impact On Production Potential Of Sheep
by Zia ur Rehman, Qazi | Dr. Asif Rabbani | Dr. Muhammad | Dr. Muhammad Afzal | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1998Dissertation note: A study of gastro intestinal helminth parasites of sheep and goats of District Peshawar has been conducted, covering a period of 4 months (May to August, 1998). During this study incidence of GIT. helminths and their impact on production potential (weight gain) of sheep and goats was studied. Random collection of samples were made from Peshawar slaughter house and a total of 40 guts each in sheep and goats were examined and following species of helminths were recovered and identified. Oesophagostomum columbianum, 0. venulosum, Trichuris parvespiculum, Haemonchus contortus, Chabertia ovina, Bunostornum trigonocephalum, Nematodirus spathiger, Moniezia benedeni, Moniezia expansa, Trichuris lani, T. globulosa, Cysticercus tenuicollis.
Only two species of cestodes and nine species of nematodes were recorded during the study. However no trematode specie was recorded. Amongst the nematodes Trichiurisparuespiculum infection was recorded to be the highest in sheep and goats with an incidence of 64.70% and 73.33% respectively. The incidence of Haemonchus contortus was 24.19 and 53.33% in sheep and goats respectively. The worm burden ranged from 1-470 in sheep and 3-141 in goats respectively.
Out of total gastro intestinal tracts examined (40 each in sheep and goats), 73.33% and 60.29% were found positive for mixed infection in sheep and goats respectively.
In order to assess the effect of GIT helminths on live weight gain and its measurement in terms of economics of the above mentioned animals, 0 Hasthnagri sheep and 30 beetal goats were divided into two groups (control vs. treated by Nilzan Plus of ICI). The average daily weight gain in sheep group was 83.78 gms while in goats it. was 85.26 gms per animal. On comparison with treated group, the increase weight gain was 3.03 kg and 2.56 kg in sheep and goats respectively at the end of the experiment. Calculating the increase on live weight basis Rs.234.5 and 158 per animal were earned more in sheep and goats respectively. After deducting the cost of anthelmintic treatment the net gain Rs.222.5 and Rs.146 per animal in sheep and goats. During this study a progressive decrease in faecal egg counts with increasing age was observed both in sheep and goats.
Statistical analysis by using un-paired "t" test confirmed that there was significant differences in weight gain and faecal egg counts, between control and treated groups of sheep and goats.
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70.
Study On The Prevalence Of Genus Trypanosoma And Effects Of Natural Infection On Various Blood Parameters In Dogs
by Afzal, M | Dr. Kamran Ashraf | Dr. Asif Rabbani | Dr. M. sarwar | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1998Dissertation note: The study was conducted to determine the prevalence of genus trypanosome and the effects of natural infection on some blood parameters in dogs.
For this purpose blood samples of 500 pet dogs (250 adult dogs and 250 pups) were examined at Dog Hospital, College of Veterinary Sciences, Lahore. Giemsa staining procedure was admpted to stain glass slides having blood smears made from blood samples.
Out of 500 ogs, 30 dog were found positive for tryponosomiasis. It revealed that only 6% prevalence of trypanosomiasis in dogs was estimated. In case of adult dogs out of 250 dogs, 13 dogs (5.2%) and in pups out of 250 dogs, 17 dog (6.2%) were found trypanosome infected. So pups were found more susceptible as compared to adult dogs. During research it was also noticed that mot of the dogs which were found positive being kept with horses.
Then blood samples of 10 adult dogs and pups each which wre positive for trypanosomiasis and 10 adult dogs and pups each which were healty (as control) were further examined for the estimation of haemoglobin paced cell volum, erythrocytes sedimentation rate and total serum protein to check the effects of natural infection on mentioned blood parameters.
The blood parameters of trypanosome infected and healthy adult dogs were compared and similarly blood parameters of trypanosome infected and healthy pups were compared. For this comparison unpaired 't' test was applied. By calculation it was found that haemoglobin, packed cell volume and total serum protein of trypanosome infected adult dogs and pups were significantly decreased as compared to healthy adult dogs and pups, respectively. But the erythrocytes sedimentation rate was found significlantly increased in case of trypanosome infected adult dogs and pups as compared to healty, adult dogs and pups.
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71.
Comparative Studies On The Incidence Of Gastro Intestinal Parasites Of Sheep And Goats And Their Effect On Different Blood
by Najeeb ur Rehman | Dr. M. Afzal | Dr. Haji Ahmad Hashmi | Dr. M. Akram | Faculty of Veterinary Sciences.
Material type: ; Format:
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; Literary form:
not fiction
Publisher: 1998Dissertation note: A study of gastro-intestinal parasitism alongwith isolation and identification of the parasite species affecting Demani sheep and goats in and around district Dera Ismail Khan was conducted in the months of May, June, July and August, 1998. For this purpose 96 gastro-intestinal tracts of sheep and goats (48 from each) were collected from the slaughter houses of D.I. Khan and adjoining areas.
The overall incidence of gastro-intestinal parasitism in sheep and goats was found to be 67.60 and 55.81%, respectively. Overall incidence of Trernatodes (22.91 and 18.75%), Cestodes (25 and 33.33%) and Nematodes (52.08 and 47.91%) was found in the said animals, respectively.
Month-wise prevalence of gastro-intestinal parasitism was also carried out which was the highest in the month of August. Fifteen different species of parasites were isolated and identified which include:
Paramphi stomum cervi (18.75 and 12.5%), Cotylophoron cotylophorum (4.16 and 6.25%), Moniezia expansa (16.66 and 10.41%), Moniezia benedeni (6.25 and 16.66%), Avitellina centripunctata (0 and 16.06%), Haernonchus contortus (27.08 and 14.58%), Oesophagostomum venulosum (8.33 and 0%), Trichuris gyj (20.83 and 31.25%), Ostertagia circumcincta (8.33 and 10.41%), Trichostrongvlus colubriformis (6.25 and 0%), Trichostrongvlus vitrinus (6.16 and 6.25%), Trichostron&vlus axel (2.08 and 8.33%), Nematodirus spathiger (12.50 and 0%), Cooperia curticel (18.75 and 0%), Strongvloides papillosus (6.25 and 0%) in sheep and goats, respectively.
The relationship between the seasonal conditions and occurrence of gastro intestinal parasitism showed close correlation.
The findings of haematological studies showed that there was decrease in haernoglobin contents, TEC, PCV, TLC, and increase in ESR both in sheep and goats under the effect of gastro intestinal parasitism. The values of differential leukocytic count were variable both sheep and goats. Most of the haematological disturbances of different blood parameters in both sheep and goats were proved to be statistically significant, particularly TEC, Hb level and ESR. Whereas the percentage of basophils and monocytes was not affected.
From the results of the presents study, it may be concluded
that the incidence of gastro-intestinal parasitism in sheep and goats warrants that rational treatment and preventive measures must be adopted to safeguard our valuable livestock from these serious parasitic infections. It is also evident from the results that gastrointestinal parasitism adversely affects the blood components in sheep and goats which may lead to anaemia, loss of growth and loss of production resulting into depreciation of quality of milk, meat and wool.
Therefore it should be considered as top priority to educate the farmers for following a strict deworming programme amongst their animals to get rid of detrimental parasites. This will ultimately be useful in producing healthy and profitable livestock, which will not only fulfill our protein requirements but also help for foreign exchange earnings.
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72.
Studies On The Taxonomy And Prevalence Of Eimeria Species And Their Effect On Total Serum Protein In Commercial Layers
by Usman Naqi | Dr. Haji Ahmad Hashmi | Dr. M. Afzal | Dr. Shakil Akhtar Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1998Dissertation note: The present project was designed to study the prevalence of Eimerian species in layers of different age groups arid breeds. For the purpose, 500 carcasses of layers were obtained from various sources and brought to the College of Veterinary Sciences Lahore, and opened in Paraisitology Laboratory. Different parts of intestine were examined for the presence of Eimerian species. In addition, to asses the general health status of birds, relationship between oocyst count and total serum protein, 30 different layer farms were selected randomly and from cacti farm 5 birds were subjected to faecal arid blood examination. The faecal samples were checked for the presence of Eimerian. oocysts arid their count par gram of faeces. The serum wan separated from the collected blood sample and total serum protein value was calculated. The managemental factor of these 30 Farms were also recorded.
Five species were isolated from the 500 carcasses on the basis of site of infection, oocyst shape, size, length/width ratio and sporulation time. These specie5 included Simeria tenella 32.40%, C. acervulina 29.62%, C. necatrix 15.68%, C. brunetti 13.59% and C. maxima G.71@.
Out of 30 farms, 13 farms were found positive for coccidiosis. Correlation was found between the values of oocyst count and total serum protein. Five farms showed positive correlation which indicated that there was no significant difference between oocyst count and total serum protein values. Eight farms showed negative correlation indicating significant difference between the relavtive values of oocyst count and total serum protein.
It was found that layers between 3-9 weeks of age were most susceptible to coccidiosis. ESb3 was found to be the most effective and commonly used drug. Among different types of litter being used in the farms, rice husk was found to be the best bedding. Babcock was found to be the most resistant breed in layers. It was also observed that inadequate space provided to the birds, uncontrolled temperature of the farm and inadequate number of drinkers and feeders favoured the infection. Biosecurity measures remained the most important among all the factors.
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73.
A Study On The Effect Of Experimentally Induced Coccidiosis On Weight Gain And Haematological Parameters And Its Control In Qualis
by Abdul Razzaq | Dr. Asif Rabbani | Dr | Dr. Kamran Ashraf | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1999Dissertation note: The present study was conducted to see the effects of the experimentally induced coccidiosis on weight gain and some blood parameters and its control with medication and vaccination. For this purpose, 150 day-old quail chicks were purchased from the local hatchery and were reared under control managemental and coccidiosis free condition upto the 42 days of experiment. A commercial coccidiostat free feed and drinking water were provided ci libitum throughout the experiment. The birds were divided in five separate groups i.e. A, B, C, D and E, each comprising of 30 birds. Group A was kept as a non-infected, non-medicated and non- vaccinated. The chicks of groups B, C, D and E, were administered 50,000 oocysts of. tenella directly into the crop at the 18 days of age, the chicks in group C were immunized against coccidiosis with a locally prepared vaccine on 3rd and 10th day of age, while those of group D with an imported coccidiosis vaccine (Coccivac) on 7th day of age. The chicks of group E were medicated with Salinomycin on the appearance of clinical symptoms and OPG of the infected birds.
No oocysts were seen in the droppings of group A chicks, throughout the experiment, while group B, chicks had the highest OPG count after induction of infection, the highest peak being in the 3rd week post-infection.
The highest mortality recorded in the infected B group and low in other infected C, D and E groups and highest mortality in 3rd week of induced infection in all infected groups.
The birds in groups A and E attained highest weight gain as compared with groups B, C and D but the difference was non significant.
The lowest Hb and TEC values were recorded in infected group B, while in other infected groups C, D and E both these values were also decreased but comparatively lesser than B group. Similarly, values of TLC were increased in infected group B as compared with other infected groups i.e. C, D and E and the difference was statistically significant.
In case of differential leukocyte counts there were increased heterophil and monocytes values in group B, while decreased values lymphocytes, basophils and eosinophils were recorded in other infected groups than the other i.e. C, D and E.
The postmortem lesions observed were swollen intestines petechial haemorrhages on the wall of the intestine and dehydrated liver and lungs in infected carcasses.
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74.
Incidence Of Molluscan Intermediate Host And Faecal Diagnosis Of Bovine Schistosomiasis In And Around Lahore
by Naeem Irshad | Dr. Haji Ahmad Hashmi | Dr. Kamran Ashraf | Dr. Khalid | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1998Dissertation note: An investigation was undertaken to find out the prevalence of molluscan intermediate host, identification of the infected and non infected snails harbouring the intermediate stages of Schistosoma bovis and the role of coprological examination in the diagnosis of bovine schistosomiasis.
Four places in and around Lahore were selected for the purpose of collection of snails, isolation of cercariae from these snails and collection of faecal samples for coprological examination Four hundred snails were collected, 100 from each of 3 ponds and a natural habitat. The Snails were collected fortnightly from October 1997 to December 1997.
Prevalence of Lymneae acuminate, L. luteola, Indoplanorbis exustus, Physa acuta Melania tuberculata and Gyralaus was found to be 37.75%, 3.25%, 58%, 20.83%, 1.92% and 1.33%, respectively. Only Indoplanobis exustus was infected with intermediate stage of schistosomes, i.e. C. nudifurca and C. indice with total infection of 12792 (C nudifurca) and 7930 (C.indice) in Bansinager fish pond and Mustafabad drain Kahana, Distt. Lahore during the period from October, 1997 to December, 1997. Out of total 200 faecal samples, 32% were positive for Schistosoma infection with 3 species of genus. The positive cases showed S. bovis (100%), S. Japonicum (75%) and S. nasalis (7.8%).
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75.
A Study On The Effect Of Experimentally Induced Caecal Coccidiosis On Weight Gain Haematological Parameters In Broilers
by Irfan Ullah Khan | Dr.Haji Ahmad Hashmi | Dr.Muhammad Afzal | Dr.Shakil | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1999Dissertation note: The study was designed to observe the effect of experimentally induced coccidiosis on weight gain, FCR and somc blood parameters in broilers. For this purpose 90 day old broiler chicks were obtained from a local hatchery and reared under controlled managemental, coccidia free conditions upto 42 days of age. A commercial coccidiostat free feed and water were provided ad Jib. The birds were divided into three groups A, 13 and C, comprising of 30 birds each. The birds in group A were kept as non-infected, un-medicated control, the group B as infected and unniedicated, while group C as infected and treated with salinomycin (Coxistac 6%; Pfizer). Coxistac 6% at a doze rate of 50 grams per feed bag was provided and the resufts were studied. The chicks of group 13 and C were given oral dozes of 40,000 sporulated oocysts of jjmeria ej.eJi at the age of day 20 and 35.
There was no oocyst excretion in faeces of birds in group A, OPG of faeces in group B was higher than in group C. The OPG of faeces reached the peak during 2nd week post-infection in both the infected groups. 'Phe higher mortalit.y in the infected group 13 (20%) than in group-C (1 0%) was observed, while no mortality was recorded in group A. On postmortem it was observed that the caeca were swollen, on incision petcchial hacmorrhagcs were seen on walls of the caeca and lungs and liver of the infected carcasses were dehydrated and pale.
The group A showed highest weight gain than groups B and C. however, the group C (infected treated) showed better weight gains than group 13 (Infected). Feed conversion ratio in groups A, 13 and C were significantly different. Group A being uninfected gave the best FCR followed by birds in group C, which were medicated while birds in group B gave the poorest values.
The haemoglobin and TEC values in the infected group B were low and were normal for group A. However, the birds of group C also showed lower values as compared to group A. It was observed that the percentage of heterophils and monocytes increased, while the number of lymphocytes, basophils and eosinophils decreased in the blood of the infected groups.
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76.
The Prevalence & Control Of Natural Infestations Of Ectoparasites & Their Effect On Blood Parameters On Indigenous Birds
by Irfan Mueed, M | Dr.Kamran Ashraf | Dr. Muhammad Afzall | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2000Dissertation note: A study was conducted to record the incidence and identification of various species of ectoparasites (ticks, lice, mites), and to find out the efficacy of ivermectin against ectoparasites in indigenous poultry. A total of 2000 indigenous birds were examined, during June to August, 1999 and out of these 1035 were found infested with ectoparasites, showing an overall infestation rate of 51.75%.
The infestation rate of ticks, lice, mites and mixed infestation (ticks and lice) was recorded as 6, 16.5, 2.25 and 27 percent, respectively. The species of ectoparasites identified were: Argas persicus (fowl tick), Menopon gallinae, Menacanthus stramineus, Liveurus caponis, Goniodes gigas (lice) and Cnemidoco1)tes mutans (Scaly Leg Mite).
In order to assess the efficacy of ivermectin (Ivomec) one hundred and fifty naturally infested birds with different species of ectoparasites were selected for treatment trials and divided into three groups i.e. A, Band C, each comprising of 50 birds. The birds of group A and B were the test groups and group C served as control group.
Fifty birds of group A given ivermectin intramuscularly at a dose rate of 200Ji/kg (0.2 mg/kg) body weight and 50 birds were treated with ivermectin orally at the same dose rate of 10 mg/5 gallons of drinking water as for group A. The efficacy of the drug was determined on the basis of negative skin scrapings and absence of other ectoparasites (lice, ticks) from the body of the test group birds as recorded on day zero, 14th and 28 of post-medication of ivermectin. The cure percentage recorded in group A and B on day 28 of the experiment was 94 and, 90 percent in birds treated by intramuscular injection and oral treatment of the drug, resp~ctively.
Haematological examinations were made to compare the blood picture of Infested birds treated with ivermectin and birds infested with ectoparasites and kept as untreated control. For this purpose blood samples from treated and untreated infested birds were examined. Blood parameters studied were: Total erythrocytic count (TEC), Packed cell volume (PCV), haemoglobin (Hb) Crythrocyte sedimentation rate (ESR), Differential leukocytic count DLC). The results of haemotological examinations showed a significant decrease in erythrocytic count, haejmoglobin, packed cell volume and rise in erythrocyte sedimentation rate and increase in differential leukocytic count of treated birds as compared to infested birds.
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77.
The Prevalence And Taxonomy Of Toxocara Canis Alongwith Associated Haemotological Changes In Naturally
by Chatha, Capt. Muhammad Athar | Dr.Asif Rabbani | Dr.Muhammad Afzal | Faculty of Veterinary Sciences.
Material type: ; Format:
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; Literary form:
not fiction
Publisher: 2000Dissertation note: Toxocariasis is a common disease of carnivores throughout the world and is caused by Toxocara canis.The larvae of the parasite have been identified as the most frequent etiologic agent of Visceral larva migrans, which is primarily a paediatric disease.
In view of the zoonotic importance of the parasite, the present study was conducted to assess the incidence, taxonomy of the species of the genus Toxocara and their effects on various blood parameters of the infected canines.
For above purpose, samples were collected from dog hospital, College of Veterinary Sciences, the faecal and blood samples were stored in the refrigerator, of Parasitology Laboratory (C. V. S. Lahore).
Only those dogs which had high prevalence in Toxocara canis were examined by different faecal examination methods and were selected to get mature worms, after killing the dogs. Preservation and clearing of worms were carried out by adopting the prescribed methods. The taxonomy of the species of the genus Toxocara was studied with the help of morphological keys.
The collective over all prevalence of different types of Toxocara canis recorded in stray and pet dogs was 39 5% The species of Toxocara identified in our area were: Toxocara canis, and Toxocara Leonina.
Blood of infected dogs were exposed to various haematological studies including Fib estimation, erythrocyte sedimentation rate (ESR), packed cell volume (PCV), total erythrocyte count (TEC), total leukocyte count (TLC) and differential leukocyte count (DLC).
Haematological examinations were made to compare the blood picture of healthy and infected dogs. For this purpose , blood samples each from healthy and infected dogs were examined.
The results of haematological examination showed a significant decrease in haemoglobin estimastion, total erythrocyte count, packed cell volume Whereas a significant increase in erythrocyte sedimentation rate total leukocyte count and differential leukocyte count , like neutrophils , eosinophils and very little increase in basophils was also seen.
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78.
Taxonomy & Chemotherapeutic Control Of Prevalent Helminth Species In Indigenous Poultry
by Abid Farid | Dr.Kamran Ashraf | Dr. Asif Rabbani | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2000Dissertation note: The present study was conducted to identify the helminth infestations, compare the drug efficacy percentage of I vermectin and Albandazole, effect of chemotherapy on haematology in indigenous poultry.
For this purpose, one hundred and sixty (160) positive birds were purchased from market and backyard farms, divided in four groups A, il, C, and D, kept in experimental rooms of College of Veterinary Sciences, Lahore.
Eggs per gram (EPG) of faeces of all the four groups was done at zero day of the experiment which were, 2307, 2000, 2050, 2325, in groups A , B, C, D, respectively. Groups A, Band C, were treated with Ivermectin and Albandazole (using 1% Ivomec Injection and Farbenda 10% solution).
After medication EPG was carried out of all four groups (A, D, C, D) at different days of experiment. The results were as, the EPG at 7th day 825, 667, 700, 2750, at 14th day 100, 250, 325, 3000, on
21st day. It was 70, 155, 237, 3500, and at 28th day the EPG. recorded was 45, 120, 185, 3600 respectively.
At end of the experiment, the drug efficacies were recorded, for group A, "it was 98%. In group B,94%, and for group C, it was 90% while group D, was untreated control.
The taxonomy of the slaughtered birds of group D, was done at the end of the experiment. By collecting GIT, and trachea of all the forty birds of the group D. Identifications were made after making permanent mounts of recovered helminths. Four species of nematodes were recovered from indigenous poul try. Ascaridia galli was the most common species of nematodes recovered from small intestine, while Heterikis gallinae and Subulura brumpti were isolated from the caeca of the birds. Syngamus trachea was the only respiratory tract nematode species which was recovered from trachea of the fowls. The incidence of Ascaridia gglli, Heterikis lZallinae, Subulura brumpti, Syngamus trachea were 57.5, 40, 20 and 2.5 percent respectively.
Six species of Cestode parasites were isolated from the small intesliine of Desi fowl, Raillictina tetragona, Raillictinna costcillus, Raillietina echinobothrida, Cotugnia dignopora, Choanotaenia infundibulum, Amoebotaenia sphenoides and the incidence recorded, 5235, 37.5, 20, 27.5, 22.5 and 2.5 percent, respectively
Availability: Items available for loan: UVAS Library [Call number: 0638,T] (1).
79.
Comparative Study On Immuno Modulatory Effect Of Anticoccidial Vaccines And A Coccidiostat On Nd Vaccinated Broiler Chicks
by Khalid Hazeen, M | Muhammad Afzad | Haji Ahmad Hashmi | Khushi | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 2000Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0646,T] (1).
80.
Prevalence And Taxonomical Studies On Haemoparasites Of Equine And Their Effect On Blood Parameters During Winter
by Wasim Shehzad | Haji Ahmad Hashmi | Asim Aslam | Dr. Kamran Ashraf | Faculty of Veterinary Sciences.
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Publisher: 2000Dissertation note: The present study was conducted between November 1999 and January 2000 to find out the prevalence and to study the taxonomy of haemoparasites of horses. In addition, their effect on haematological parameters was also studied.
A total of 40 out of 435 horses were found positive for haemoparasites. The prevalence recorded was 9.20%. Out of positive animals 22(5.06%) were positive for babesia species, of which 10 (45.45%) were infected with B. equi, 6 (27.27%) were infected with B. caballi and 6 (27.27%) were positive with mixed infection of B.qui and B. caballi. out of remaining positive cases, 14 (3.22%) were positive for T.evansi and 4 (0.92%) were positive for P.multipapillosa.
Animals of group A, belonged to all the places of other groups. Animals of group B showed 10.52 % prevalence for haemoparasites, Babesia species was present in 6 (6.3l%) and T.envansi was present in 4 (4.2l%) animals respectively. Horses of group C showed prevalence as 14.28% haemoparasites out of which 6(8.57%) were positive for babesia species, and 4 (5.71%) positive for T.evansi . Similarly, animals in group D, indicated 11.11 % prevalence of haemoparasites including babesia species 3 (3.33%), T.evansi 3 (3.3%) and P.multipapillosa, 4 (4.44%) respectively. Ten out of 180 (5.56%) horses of group E were positive for haemoparasites and 7 (3.89%) were positive for Babesia spp. and 3 (1.66%) were positive for I.evansi.
Haematological examination revealed a significant decrease (P<0.05) in the total erythrocyte count, total leukocyte count, haemoglobin estimation, packed cell volume, neutrophils and basophils in the infected horses as compared with healthy horses. However, there was a significant increase (P<0.05) in lymphocytes and eosinophils in the infected horses as compared with healthy horses of group A.
From the results of the present study it may be concluded that the horses belonging to the poor community need due attention for the control of haemoparasite diseases. Services must be extended to educate owners of these Tonga ponies for the control of haemoparasitic diseases and their vectors.
Availability: Items available for loan: UVAS Library [Call number: 0648,T] (1).
81.
Comparative Prevalence And Taxonomy Of Amphistomes Infecting Cattle And Buffaloes And Their Effect On Various Blood Components
by Fareed Ahmad | Haji Ahmad Hashmi | Dr. Kamran Ashraf | Dr.Asinm Aslam | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 2000Dissertation note: The present study was carried out to determine the incidence and taxonomy of various gastric amphistomes in buffaloes and cattle. I addition the effect of these amphistomes on different haematological parameters (TEC, PCV, Hb, ESR and DLC) was carried out.
For this purpose 151 compound stomachs (70 buffaloes and 81 cattle) of slaughtered ruminants were examined at Lahore abattoirs. The worms were collected and identified and taxonomical findings were recorded. In addition, faecal samples from the positive cases were also collected and examined to record the EPG count. The blood samples were also collected from the above mentioned animals to study the effect on different haematological parameters. The animals awaiting slaughter were selected irrespective of age and sex.
A total of 70 buffaloes and 81 cattle had to be examined to obtain the 50 positive buffaloes and 50 positive cattle, infected with different species of amphistomes. The overall incidence of amphistomes was found to be 71.42% in buffaloes and 61.72% in cattle.
The various species of gastric trêmatodes identified were as follow i.e. Paramphistomum cervi, Cotylophoron cotylophorum, Gastrothvlax crumenifer and Carmverius spatiosus. The incidence of . cervi infection was higher both in buffaloes (54.48%) and in cattle (44.34%) as compared with the other species of the family Paramphistomatidae. The incidence of Cotvlophoron cotylophorum was found to be 50% in buffaloes and 39.5% in cattle. The incidence rate of Gastrothylax crumenifer and Carmyerius spatiosus was found to be 38.57% and 28.57% in buffaloes while 29.62% and 19.75% in cattle respectively.
The EPG of affected buffaloes and cattle carcasses was also undertaken and the average EPG recorded was 44.67 eggs/gram in buffaloes and 39.78 eggs/gram in cattle.
Haematological findings revealed that the Hb, PCV and TEC were decreased while the neutrophils and eosinophils were increased both in buffaloes and cattle suffering from amphistomiasis.
Availability: Items available for loan: UVAS Library [Call number: 0657,T] (1).
82.
Comparative Immune Response Of Anticoccidial Monovalent And Polyvalent Vaccines On Nd Vaccinated Broiler
by Wajiha Maqsood | Dr.Haji Ahmad Hashmi | Dr.Muhammad | Dr.Muhammad Afzal | Faculty of Veterinary Sciences.
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Publisher: 2001Dissertation note: The study was conducted to estimate the immune response of the three anticoccidial virus vaccines on NDV vaccinated broiler chicks. The vaccines used were, Eirneria tenella vaccine (ETV), Immucox and Coccivac. For this purpose a total of one hundred and five chicks were randomly divided into five groups, namely group A, B, C, D and E on day I Each group consisting of twenty one birds. All the chicks except those in group A, were occulonasally vaccinated with Newcastle disease virus vaccine using Mukteswar strain of virus on day I and 2 1
Chicks in group A were kept as non-i nfectecl, non-vaccinated controls. Group B was non-infected, hut vaccinated with NDV. group C was also vaccinated with Eimeria tenella vaccine on days 3 and 10 of age. Group D was administered with single dose of Immucox on day 5th of age and, group E was administered with Coccivac on day 6111.
After vaccination weekly mean weightgains, mean oocysts per gms of faeces (OPG), and mean feed conversion ratio of chicks in all the groups were recorded. Regular sera samples were also collected from the blood obtained from birds of all the groups, after administration of second dose of NDV on day 21 of age. All the sera samples were analyzed by using Haernagglutination (HA) and Haemagglutination inhibition (HI) tests.
obtained from birds of all the groups, after administration of second dose of NDV on day 21 of age. All the sea samples were analyzed by using Haemagglutination (HA) and Haemagglutination inhibition (HI) tests.
It was observed that no coccidiosis could be established in chicks of groups A, B and C. However oocysts were observed in the faecal material of chicks of group D and E after one week of administration of anticoccidial vaccines (immucox and coccivac) respectively. But OPG was gradually increase after second week of vaccination and one bird in group D died due to coccidiosis, OPG count reached to a peak after second week of vaccination and one bird in group D and one in group F died due to coccidiosis.
Mean weight gains per chick on day 1 was 41, 42, 40, 39 and 38 of groups A, B, C, D and F respectively. however the mean body weights were 1746, gms 1848, 1725, 1710 and 1695 gms for chicks in groups A, B, C, D and E respectively at Group B showed significant weight gain as compared to other groups and their difference of weight gain from group B was, 103, 123, 138, and 153 gms of groups A, C, D and F respectively. (P > 0.05).
Feed conversion ratio of groups A. B, C, D and F was 1.85, 1.99, 1 .88, 1.77 and 1.78 respectively at the end of experiment. Group B and C showed the best FCR as compared to other groups of the same age. But statistically the differences were non-signiFicant amongst all the test groups.
Morphometeric analysis of immuno modulatory organs revealed that group B showed highest organ weight and the organ body ratio as compared to other groups of same age at the end of experiment Besides group B, group C also showed the second highest of the organ body weights and the organ body ratios, as compared to other anticoccidial vaccinated groups D and E and among them group C showed the highest of organ weight and organ body ratio.
Antibody analysis revealed that geometric mean titre (GMT) in the chicks of group B (NDV vaccinated control group) showed the highest value (73.3) as compared to other groups of same age at the end of experiment. However group C showed the second highest value (68.6) on 49th day. Where as other anticoccidial vaccinated groups D and E showed their GMT value as 21.1 and 22.6 respectively, and antibody titre of group A (non-infected non vaccinated control group) was zero at the end of experiment.
Availability: Items available for loan: UVAS Library [Call number: 0660,T] (1).
83.
Study On The Comparative Efficacy Of Phenothiazine, Levamisole And Ivermetin Against Gastro Intestinal Nematodes In Horses
by Ashfaq Ullah Hashmi | Dr . Nisar Ahmad | Dr . Kamran Ashraf | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 2001Dissertation note: The study was undertaken to find out the comparative efficacy of Phenothiazine, levamisole and Ivermectin against GIT nematodes in horses at Lahore. For the purpose one hundred horses irrespective 'of age, breed and sex were exam for the presence of various species of gastro-intestinal nematodes in horses. The coprological examination showed a positive percentage of 64% while 36 cases (36%) were found to be negative. The positive cases had mixed infestation of large and small strongyIids, Parascaris equorum and Oxyuris equi species. Forty naturally infested horses divided in four groups (A, B, C and D), were selected for the drug trial. The drugs used were Phenothiazine @ 55 mg/kg body weight, levamisole @ 7.5 mg/kg body weight (1 mI/2 kg b.w) Ivermectin @ 0.2 mg/kg body weight to the groups A, B, C and D, while the animals of group D acted as non-medicated control.
The mean EPG count was carried out pre-medication and post medication of the said anthelmintics.
Based upon EPG count, conducted on 3rd, 7th and 14th day for each anthelmintic; it was revealed that the mean EPG count of group A (Phenothiazine) showed 62.99, 82.67 and 95.27% reduction in ova output while the animals of group D (medicated with levamisole exhibited 53, 82 and 93% reduction in the said equines). The group C (medicated with Ivermectin showed reduction ofEPG as 79.83,95.16 and 100% on 3rd, 7th and 14th day post medication).
From the results of this study it is concluded that Ivermectin was the most effective as compared to Phenothiazine and levamisole. Therefore, it is stressed that Ivermectin should be used in equines for the effective control of gastro intestinal nematodes. The drug is quite safe and without any adverse effects on these animals.
Availability: Items available for loan: UVAS Library [Call number: 0682,T] (1).
84.
Epidemiological Studies On The Prevalence Of Coccidiosis In Commercial Broilers And Its Relationship With Other Diseases
by Umber Rauf | Dr . Asif Rabbani | Dr . Khalid Saeed | Dr. Shakil | Faculty of Veterinary Sciences.
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Publisher: 1999Dissertation note: In this epidemiological study 26.48% birds of 26.82% farms were found affected with coccidiosis. Six species of Eimeria, E. acervulina. E. mitis, E. tenella, E. necatrix, E. praecox and E. mivati were observed. Number of oocysts in litter varied with the age of birds. Concurrent infections were detected in 80.68% cases, which included infectious coryza 21.95%, aflatoxicosis 27.45%, infectious bursal disease 16.94%, chronic respiratory disease 12.54%, Newcastle disease 10.16%, hydropericardium syndrome 9.83%, Escherichia coli 9.15% and Salmonella 8.13% infections. In the month of July and August , incidence of coccidiosis was high. Maximum incidence of disease was observed from 4th to 6th week while no disease incidence was noted during 1st and 8th week. It was also noted that the disease had drastic effects on Newcastle disease antibody titer.
Availability: Items available for loan: UVAS Library [Call number: 0691,T] (1).
85.
Estimation Of Blood Haemoglobin, Serum Iron And Magnesium Level In Clinical Cases Of Ovine Haemonchosis
by Haneef ur Rehman | Dr . Asif Rabbani | Dr . Mubashar Saeed Mian | Dr . Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1985Dissertation note: Haemonchosis is a serious helminthic infection of sheep and goats resulting in the mortality due to excessive blood loss and causes great economic losses. The objective of the present study was to determine the effect of natural infection of Haemonchus contortus in sheep upon blood haemo- globin, serum iron and magnesium level so that it could aid in accurate diagnosis and effective therapeutic measures. Infection was confirmed through faecal examination. Eggs per gram of faeces were calculated by McMaster egg counting technique. To see the effect of severity of infection on these blood components, blood samples of positive cases were taken from jugular vein.
Blood haemoglobin, serum iron and magnesium were estimated by using standard diagnostic kits with spectrophotometer. A decreased level of haemoglobin and serum iron was directly proportional to the severity of infection. However, no alteration in serum magnesium level was observed in infected and non-infected animals.
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86.
Studies On The Babesia Canis And Ticks Of Dogs
by Abdul Haseeb Khan | Haji Ahmad Hashmi | Dr.Khalid Pervez | Dr.Khalid Saeed | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 2001Dissertation note: The present study was conducted from September to November, 2000 to find out the prevalence and taxonomy of ticks and Babesia canis of dogs. In addition, their effect on haematological parameters was also studied. To find out the prevalence, 759 dogs were included in the study. The samples were collected from the dogs brought to Dog Hospital, College of Veterinary Sciences, Lahore, Private Veterinary clinics and from different Private Kennels present in and around Lahore.
Out of the total 759 dogs, 205 (27 %) dogs were found positive for single species of ticks i.e. Rhipicephalus sanguineous and 179 (23.5%) dogs were found positive for Babesia canis. Seventy four (9.7%) dogs were infested with only ticks and 48 (6.3%) dogs were found positive for only Babesia canis. Out of total 205 ticks infested dogs, 131 (63.3%) were found positive for Babesia canis.
For haematological study from the total examined dogs 80 (60 infected, 20 healthy) were selected and divided in to four groups A (mixed infection of ticks and Babesia), B (ticks only), C (Babesia only) and D (non-infected). Each group comprising of 20 animals.
The total erythrocytic count were 2.5x106±0.28/,il, 3.2x106±0.21/iil, 3.6x106±0.13/id and 4.9x106±0.18/j.d of group A, B, C and ID respectively. There was significant difference amongst group A, B, and C as compared to the control group-D. A significant difference amongst A-B, A-C was also noted. But no significant difference between group B and C could be recorded.
Hemoglobin estimation in group A was 7.1±0.33 g %, in B was 9.2±0.30 g % and in group C was 9.8±0.30 g %. It was revealed that there was significant decrease in hemoglobin contents as compared to group 'D' which showed 12.01±0.6 g %. There was significant difference between group A-B and A-C, but no significant difference between group B-C (P<0.05) was noted.
The animals belonging to group A, B, C and D had ESR as 40.15±3 mm/hour, 32.25±0.17 mm/hour, 25.2±1.1 mm/hour, and 16.3±1.3 mm/hour respectively. There was significant difference amongst the groups A, B, C as compared to group D. There were also significant difference amongst groups A-B, A-C and B-C (P<0.05). The RBC of the groups A, B, and C were found crenated and macrocytic hypochromic.
The overall results showed that effects of mixed infection of ticks and Babesia on haematology were more adverse as compared to the single infections of ticks or Babesia.
Availability: Items available for loan: UVAS Library [Call number: 0712,T] (1).
87.
Biocontrol Of Caecal Coccidiosis In Broilers
by Nadeem Afzal, M | Dr.Haji Ahmad Hashmi | Dr.Muhammad Sarwar Khan | Dr.Nisar Ahmad | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 2001Dissertation note: The present study was designed to estimate the efficacy of two types of monovalent . tenella vaccines i.e. Formalin treated . tenella oocysts for 48 (FEV-48) and/or 96 (FEV-96) hours and irradiated . tenella vaccine. For estimation of the efficacy of the said vaccines, OPG counts weight gain, FCR and mortality records were compared amongst the control and test groups.
A total of 105 day-old broilers were equally divided into 5 different groups namely; A, B, C, D and E on day 3, each group comprising of 21 chicks. NDV vaccine was administered on day land 21 of age of chicks of all the groups. Group A acted as non-infected control, B as infected control, C was vaccinated with FEV-48, D was vaccinated with FEV-96 and E was vaccinated with 1EV on day 3 and 10 of age. Spread of contaminated faeces over the bedding with . tenella oocysts was commenced on day 12 upto day 17 of age of chicks. The OPG counts were conducted on each 4th day from day 18th to 52.
The results indicated that the first observation of OPG counts (day-18) showed heavy infection in all the control (infected) and test groups. In group B, 222200 oocysts were observed on day 18 which gradually reduced to 5300 on day 50 followed by zero on day 52. Group C (FEV-48) showed 131400 OPG counts, which sharply declined on each observation and showed 100 OPG on day 38 followed by '0' afterwards and showing significant differences from the control (P < 0.05). Group D showed gradual decline like infected control upto day 34, but ironically on days 38 and 42, 196.3% and 376.4% increase was noted, respectively as compared to group B, which again declined by day 50 with significant difference. In group E the first observation showed 119460. OPG counts which abruptly declined to 75 on day 38 followed by '0' on day 42. Groups C and E showed comparable results.
Weight gains were almost equal in all the test and control groups by day 14. The difference appeared on third observation between infected control and other groups. At the end of the experiment members of groups A, C, D and E gained 31%, 14%, 11.83% and 10.92% greater weights than group B. The FCR in ranked order was shown as A, C, E, D and B, showing the efficacy of FEV-48. Mortalities occurred in groups B, C, D and E as 4 (19%), 2 (9.5%), 1 (4.76%) and 3 (14.28%), respectively.
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88.
Taxonomy And Prevalence Of Intestinal Parasites Of Sparrows (Passer Domesticus)
by Anjum Mahmood | Dr.Kamran Ashraf | Dr.Muhammad Sarwar Khan | Dr.Nisar Ahmad | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 2000Dissertation note: The study was undertaken to assess the prevalence and taxonomy of the gastro-intestinal tract helminths in 200 sparrows that were naturally parasitized. In addition, the blood examination was carried out to record the effect of worm infestation on different blood parameters namely Haemoglobin (Hb) estimation, Total leukocyte count (TLC), Packed cell volume (PCV) and Differential leukocyte count (DLC) and these values were compared with the normal values.
Two hundred sparrows purchased from the market were kept in cages in Parasitology Section, College of Veterinary Sciences, Lahore. The birds were slaughtered at due intervals. Blood was collected and gastro-intestinal tract was searched for the collection and identification of the helminthic species.
The results revealed that out of 200 sparrows, 120 had the worm infestation, showing an overall incidence as 60%. The worms collected were subjected to the taxonomical study which was carried out in the laboratory of PaLrasitology Section.
Two species namely Ascaridia galli (36%) and Heterakis gallinarum (24%) were found inhabiting the small intestine and caeca of the infested birds, respectively. No trematodes and cestodal species were recorded from the GIT of these experimental sparrows. Blood examination revealed slight increase in TLC and Heterophils, while basophils and monocytes were not affected. However, Hb levels, PCV and lymphocytes showed decrease resulting into anaemia and retarded growth of the affected birds versus non-infected ones.
The eggs per gram (EPG) ranged between 200-1000 in case of Ascaridia galli and 400-1000 in Heterakis gallinarum infestation in naturally parasitized sparrows. The main EPG count recorded was 516.666 and 660.41 in case of Ascardia galli and Heterakis gallinarum infestation, respectively.
Availability: Items available for loan: UVAS Library [Call number: 0721,T] (1).
89.
Study On Ticks And Haemoparasitic Diseases Of Local And Cross Bred Cattle In Malakand Agency
by Nazir Ahmad | Dr.Haji Ahmad Hashmi | Dr.Muhammad Sarwar Khan | Dr.Nisar Ahmad | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 2002Dissertation note: Ticks infestation and piroplasmosis are serious problems of domestic cattle. The present study was conducted to find out the prevalent species of ticks and the piroplasms transmitted by these ticks. The effect of ticks and blood parasites on various blood parameters was also studied. The parameters included: RBC count, WBC count, Haemoglobin (Hb) estimation, erythrocytic sedimentation rate (ESR) and differential leukocytic count (DLC). The study was conducted from June to August, 2001.
A total of 80 cattle (40 local nondiscript cattle and 40 crossbred) were selected. Each type was divided into four groups i.e. A, B, C and D. The cattle of group-A were free from any infestation/infection. The animals of group B were only tick infested. Animals of group-C were only piroplasm infected and the animals of group-D were infested with ticks and the piroplasms. Each group from both the breeds consisted of 10 animals.
Haemotological examination revealed a significant decrease (P<0.05) in TEC, Hb and TLC in groups B, C and D as compared to group-A relating to each breed.
The lymphocytes were decreased from normal in all test groups in both the breeds, significantly. However, neutrophil and eosinophils were increased significantly in test groups as compared to control group (A). The values of erythrocytic sedimentation rate (ESR) showed a significant increase in groups B, C and D of both types of cattle as compared to the control group (A).
The overall results showed that the effect of mixed infection on haematology was greater than the single infection of ticks or piroplasm.
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90.
A Study On The Prevalence Taxonomy And Control Of Mange Mites Infestation In Equines And Their Effect On Blood
by Arshad, M | Dr.Kamran Ashraf | Dr.Asim Aslam | Dr.Haji Ahmad Hashmi | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 2000Dissertation note: The present research project was designed to find out the prevalence, taxonomy and control of mange mites infestation in equines and to record the effects of mange on different blood parameters of the said animals.
For this purpose, a total of 200 equines suspected for mange mites infestation were examined during June August (1999). Out of these 200 suspected equines, 24 were found positive, indicating an overall prevalence of mange mites as 12%. Out of the positive animals, 20 naturally infested animals were selected for further investigation and divided into two Groups-A & B having 10 animals in each. Ten animals of Group-A were medicated with ivomac injection by (Rhone Poulenc) at a dose rate of 0.2 mg / kg body weight or (1 ml / 50 kg b.wt.) subcutaneously and the 10 infested animals of Group-B served as untreated controls. The efficacy of drug was assessed by examination of skin scrapping and the animals were re-examined at 7th, 14th and 28th day post treatment with ivomac injection. Taxonomy of the identified species was also carried out.
In addition, the effect of mange mite infestation on different blood parameters of equines was also recorded. Haematological parameter showed significant reduction in total erythrocyte count (TEC), haemoglobin (Hb) level and Packed Cell Volume (PCV) while Erythrocyte Sedimentation Rate (ESR) was increased in infected animals. Differential leukocyte count (DLC) showed reduction in the neutrophils and lymphocytes but eosinophils, basophils and monocytes were increased in number in mite infested horses.
From the results of the present study, it was concluded that the skin scrapings examination of the animals showing irritation and pruritis should be carried out on regular basis and those found positive should be given prompt acaricidal treatment. Ivomac injection @ 0.02 pg / kg body weight has proved the most beneficial as a single injection was found to be quite effective to cure the mange mites infestation in these animals. Hence, the farmers, Tonga pony owners and other equine lovers should use Ivomac Injection to treat and control the mange mites infestation in horses, mules and donkeys. This will not only result in the improvement of the health status of these animals but also increase income of the owners.
Availability: Items available for loan: UVAS Library [Call number: 0733,T] (1).
91.
Oocyst Production Potential In Experimentally Infected Broiler With Eimeria Tenella
by Khalid Younas, M | Dr.Kamran Ashraf | Dr.Haji Ahmad Hashmi | Dr.Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 2001Dissertation note: The present study was designed to observe the periodicity of oocyst shedding, the ratio of administered oocysts with the oocysts found in the faeces or intestinal contents of infected birds and effect of artificially induced coccidiosis at different ages on weight gain, FCR, OPG count, mortality and lesion scores. For this purpose 84 day-old chicks were purchased from the market and vaccinated against NDV on day 1 and 21 (Mukteswar strain). The birds were divided into 4 groups i.e. A, B, C and D comprising oC 21 birds each. Group A was kept as non-infected control. Virulent Elmeria en at a dose rate of 50,000 sporulated oocysts/bird was given to group B, C and D on day 15, 25 and 35 of age, respectively.
There was no oocyst excretion in faeces of birds in group A. OPG of faeces in group B was higher than in groups C and 1). rFIe OPO of faeces reached the peak on 6th day post-infection in infected groups, but group D again obtained the peak on 17th day postinfection. The highest caecal oocyst counts were observed in group B than C and 1) groups. It was observed that the recovery of the number of oocysts in purified isolates was 58.74%, 50.05% and 26.87% in B, C and D, respectively as compared to OPG counts. It was also noted that the caecal output of oocyst counts was always lower than the total OPG counts per chick during the period of infection in different groups.
Group A showed the highest weight gain (2155 gms) than the infected groups B (1985 grns), C (1930 gms) and D (1978 grns). Feed conversion ratio in groups A, B, C and D was significantly different showing the values as 1.62, 2.26, 1.89 and 1.82, respectively. Group A being uninfected gave the best FCR followed by the birds in groups D and C. While birds in group B gave the poorest values, it was observed that the weight of faeces per chick was almost equal in groups C (163 gms) and D (166.5 gms), but was significantly lower in B (123.5 gms). However, the highest OPG counts occured in group B and the number of oocysts acquired were the best in this group.
The highest mortality was observed in group B (28%) followed by group D (14%) and group C (10%), while no mortality was recorded in group A.
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92.
Comparative Effecacy Of Different Prophylactic Measures Against Cocidiosis In Broilers
by Faisal Masood | Dr. kamran Ashraf | Dr. Haji Ahmad Hashmi | Dr. Muhamad | Faculty of Veterinary Sciences.
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Publisher: 2000Dissertation note: The study was designed to look into the comparative efficacy of different prophylactic measure against coccidiosis in broilers. One hundred and fifty, day old broiler chickens were reared under controlled conditions. They were equally divided into five groups, on day 1, i.e. A, B, C, D and E. Group 'A' was kept as uninfected, unmedicated control whereas all other test groups B, C, D and E were infected with 30,000 sporulated virulent oocysts. Group 'B' acted as infected and unmedicated control at day 20 and 30 of age. Group 'C' acted as infected and treated with Diasul-S at the rate of 1/2 gm/lit of drinking water for 3 days and group 'D' was treated with coxicure A-K at the rate of 1/4 gm/lit drinking water for 3days. The birds of group E received locally prepared Eimeria tenella vaccine (ETV) on day 3 and 10 of age of birds.
No coccidiosis in the birds of group A and E was noted through out the experiment. The oocysts per gram (OPG) of faeces in group A and E was nil Members of group 'B' showed the highest counts than any other group. The birds of group 'C' and 'D' showed the moderate counts but towards the completion of experiment, the OPG counts gradually reduced in both groups.
Weekly average weight gain record showed that average weight gain by the birds of group 'A' were the highest as compared with all other groups. The average weight gain of group E showed the second highest weight gains. The members of group 'B' showed the lowest weight gains while the members of groups 'C' and 'D' were ranked as 3rd and 4th in weight gains. The weight gains observed in groups A, C, D and E as compared with groups B were significantly higher (PO.O5) N. P.. In groups 'A', 'C' and 'E' the morbidity was nil. While in groups 'B' 30% morbidity was observed. Group 'D' showed low morbidity and one bird died due to coccidiosis.
The best feed conversion ratio (FCR) was observed in groups 'A' and followed by groups 'E', 'C' and 'D'. The feed conversion ratio in groups 'C' and 'D' was equally good. Worst feed conversion ratio was observed in group 'B'.
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93.
Prevalence And Taxonomical Studies On Haemoparasites Of Equine And Their Effect On Blood Parameters During Winter Season
by Wasim Shehzad | Dr. Haji Ahmad Hashmi | Dr. Asim Aslam | Dr. Kamran Ashraf | Faculty of Veterinary Sciences.
Material type: ; Format:
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not fiction
Publisher: 2000Dissertation note: The present study was conducted between November 1999 and January 2000 to find out the prevalence and to study the taxonomy of haemoparasites of horses. In addition, their effect on haematological parameters was also studied.
A total of 40 out of 435 horses were found positive for haemoparasites. The prevalence recorded was 9.20%. Out of positive animals 22(5.O6%) were positive for babesia species, of which 10 (45.45%) were infected with 6 (27.27%) were infected with B.caballi and 6 (27.27%) were positive with mixed infection of and B caballi out of remaining positive cases, 14 (3 22%) were positive for T.evansi and 4 (0.92%) were positive for P.multipapillosa.
Animals of group A, belonged to all the places of other groups. Animals of group B showed 10.52 % prevalence for haemoparasites, Babesia species was present in 6 (6.31%) and T.evansi was present in 4 (4.21%) animals respectively. Horses of group C showed prevalence as 14.28% haemoparasites out of which 6(8.57%) were positive for babesia species, and 4 (5.7l%) positive for T.evansi. Similarly, animals in group D, indicated 11.11 % prevalence of haemoparasites including babesia species 3 (3.33%), T.evansi 3 (3.3%) and P.multipapiltosa, 4 (4.44%) respectively. Ten out of 180 (5.56%) horses of group E were positive for haemoparasites and 7 (3.89%) were positive for Babesia spp. and 3 (1.66%) were positive for T.evansi.
Haematological examination revealed a significant decrease (P<0.05) in the total erythrocyte count, total leukocyte count, haemoglobin estimation, packed cell volume, neutrophils and basophils in the infected horses as compared with healthy horses. However, there was a significant increase (P<0.05) in lymphocytes and eosinophils in the infected horses as compared with healthy horses of group A
From the results of the present study it may be concluded that the horses belonging to the poor community need due attention for the control of haemoparasite diseases. Services must be extended to educate owners of these Tonga ponies for the control of haemoparasitic diseases and their vectors.
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94.
Imunization Of Rabbits Against Boophilus Microplus Using Midgut And Salivary Glands
by Mohammad Asif | Dr. Khalid Saeed | Dr. H. A. Hashmi | Dr. Khushi | Faculty of Veterinary Sciences.
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Publisher: 2002Dissertation note: The current research was conducted as a simulation model in rabbits for the development of vaccines against cattle ticks Boophilus micro/us using tick salivary glands & midgut as the sources of antigens. The comparative efficacy of these vaccines was evaluated on the basis of antibody titres and tick rejection on the host in terms of mortality, decrease in egg laying and decrease in hatchability of eggs. It was found that the vaccine prepared from the midgut of B. microplus gave better results than the vaccine prepared from salivary glands. The highest percentage of dead ticks was observed in animals which were given midgut vaccine (21.82%). This vaccine also caused a significant decrease in the egg laying capacity of the ticks (35.83%). However, none of the vaccines had any significant effect on the hatchability of eggs laid by the ticks. The highest antibody titers were observed in rabbits injected with midgut vaccine (GMT=5.50), which was 45.28% higher than rabbits given salivary gland vaccine. The overall success rate for midgut vaccine, in terms of tick mortality on host, decrease in egg laying capacity and antibody titer,. was 37.06% for midgut vaccine while only 16.51% for salivary gland vaccine. The results are very encouraging and it is hoped that with the original host i.e. cattle, more satisfactory results can be achieved.
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95.
Prevalence Of Toxocariasis In Dogs And Its Treatment With Nigella Sativa Saussurea Lappa And Ivermectin
by Adnan Mushtaq | Dr. Nisar Ahmad | Dr. Kamran Ashraf | Faculty of Veterinary Sciences.
Material type: ; Format:
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; Literary form:
not fiction
Publisher: 2002Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0773,T] (1).
96.
Study On Naturtal Quine Strongylosis And Its Control
by Zahida Qadir | Dr.Khalid Saeed | Dr.H.A.Hashmi | Dr.Shakil Akhtar Khan | Faculty of Veterinary Sciences.
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Publisher: 2001Dissertation note: Two hundred equines of different age, sex and breed were subjected to coprological examination. A total of 65.51% sample were found to be infected with various helminths. Strongyles were the most common parasites and were detected in 58.5% of total faecal samples. The other species identified in this study included P. jprum (2%), Dictyocalus arnifeldi (1.5%), Gafitrodisus aegvuticus (1.5%), Anaplocepha species (1.5%), Strongvloides westeri (1.5%) and Strongyles + Ascaris (1.5%).
The higher infection rate of helminth parasites were observed in spring (78%) and in summer (82%) as compare with autumn (40%) and winter (56%). Highest average EPG of 530 was recorded in summer and lowest EPG of 161 in winter season. Age related susceptibilities indicated a high prevalence of strongylosis in equines of under 10 years of age as compared with horses of more than 10 years of age. A higher egg per gram of the faeces were also recorded in horses of 1-3 years of age (512) as compared with older horses. Males have higher prevalence of helminths (76%) as compared with female (49%). There was no difference in average egg shedding by male (304) and fema1 horses (296).
The anthelmintics efficacy of oxafax, ivomec and farbencja were also evaluated. Forty horses found positive for natural strongyle infection were randomly divided into four equal groups. Group A, B and C were dosed with Oxafax, Ivomec and Farbenda respectively as recommended by manufacturers. Group D acted as non-medicated infected control group. Percent egg reduction test indicated an efficacy of 96%, 98% and 81% for Oxafax, Ivomec and Farbenda, respectively on day 14 (post-medication). An efficacy of 100%, 96% and 86% were observed for Oxafax, Ivomec and Farbenda respectively on day 28, Statistically there was a non-significant difference in the efficacy of Oxafax, Ivomec and Farbendazole on days 14 and 28 post medication (P>O.05). However, these drugs showed a significant reduction in average EPG as compared with control group (P >0.05) on both sampling days.
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97.
Prevalence And Treatment Of Faciolosis In Buffaloes Under Farm Conditions
by Sarfraz Ahmed | Dr.H.A.Hashmi | Dr.Kamran Ashraf | Dr.Muhammad | Faculty of Veterinary Sciences.
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Publisher: 2002Dissertation note: The present study was designed to investigate the prevalence of fasciolosis in buffaloes under farm conditions, efficacy of recently introduced Homeopathic (Trematox) with Allopathic (Zanil, ICI) drug and economic losses in terms of liver damage and decrease in milk production. For this purpose, 500 faecal samples were examined for the presence of liver fluke. The prevalence was observed 15% in buffaloes under farm conditions located in Okara district. For therapeutic purpose, sixty naturally infected and 20 healthy buffaloes were selected and divided into four equal groups i.e. A, B, C and D containing 20 animals each. Anirnals of group-A (healthy animals) were given no medication. Animals of group-B were infected control group. Animals of group-C were treated with Zanil (Oxyclozanide), while animals of group-D were treated with Trematox (A horneopathetic drug).
The therapeutic efficacy of Zanil and Trematox was compared on the basis of pre- and post-medication egg counts. The animals used for this study were naturally infected buffaloes (confirmed by faecal examination). Sixty positive and 20 negative cases were selected for tlie study. rfhe faecal samples were collected directly from the rectum of the animals.
Eggs per gram of faeces (EPG) and clinical performance were monitored. Faecal samples were examined at zero, 1st, 3rd, 5th, 7th, 10th and 18th day post-treatment. Animals which remained positive on 18th day were given a second dose of the respective drug on the same day and their faecal samples were further examined on 21st and 28th day.In buffaloes treated with Trematox the efflcacy recorded was 20.45% on 3rd day, 29.54% on 5th day, 40.90% on 7th day, 47.72% on 10th day, 50% on 14th day, 54.54% on 18th dày, 56.81% on 21st day and 61.36% on 28th day.
Buffaloes treated with Zanil showed better results than Trernatox. Decrease in the faecal egg counts was 39.13% on 3rd day, 56.52% on 5th clay, 69.56% on 7th day, 78.26% on 10th day, 82% on14th day, 86.95% on 18th day, 93.47% on 21st day and 95.65% on
28th day as compared to that of zero day.
The economic losses due to liver damage recorded were 21.6 million (Rs.) annually in 600 animals. Wheras economic loss in terms of decrease in milk production in infected animals was recorded as Rs.10,800 annually per animals
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98.
Prevalence Of Parasites Of Zoonotic Importance In Public Parks Of Lahore
by Mahmood Ahmad Tahir | Dr.H.A.Hashmi | Dr.Khalid Saeed | Dr.Muhammad Sarwar Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 2003Dissertation note: To find out the prevalence of parasitic larvae/eggs of zoonotic importance at VaI1OLIS public places, 600 samples (200 canine faeces, 200 lierbage and 200 soil samples) were collected and processed according to the techniques mentioned in the text. Five samples for each material were collected fortnightly from and around five different public parks namely: "lqbal Park, Nasir Bagh, Bagh-e-Jinnah, Gulshan-e-Iqbal and Race Course Park" during the period from 1st September to 31st December, 2002. The impact of meteorological conditions on the prevalence of various parasitic eggs/larvae was studied. Forty nine (24.5%) out of 200 faecal samples were sound to he positive amongst which 11(22.45%) were positive for T. canis. and 34 (69.39%) were positive for A. caninum whereas 4 samples (8.1 %) were found positive for other parasites namely: D. canium, Trichuris vulpis, Teania spp. and Strongyloides sp. Out of 200 soil samples 29 (14.5%) were found positive for parasites amongst which 8 (27.59%) and 19 (65.5%) were positive for T canis and A. Caninum respectively, whereas only 2 (6.90%) were positive for other parasites. Out of 200 herbage samples 69 (34.5%) were positive for parasite eggs/larvae amongst which 22 (31.88%), 42 (60.86%) and 5 (7.26%) were positive for T. canis, A. can/null and other parasites respectively.
Overall contamination of parks including all types of samples was recorded as 24.5%. It was observed that during the period of study (1st September to 31st December, 2002), the contamination of parks was the highest between 1st October to 30th November, 2002 when environmental temperature became optimal (27-30°C) and humidity being 75-76%. Inspite of the fact, rainfall did not occur during the highest prevalence of contamination.
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99.
A Comparision Of Biological And Medicinal Contro Of Coccidiosis In Broilers Under Field Condition
by Mirza Waqar Baig | Dr.Kamran Ashraf | Dr.Muhammad | Prof. Dr.Haji Ahmad Hashmi | Faculty of Veterinary Sciences.
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Publisher: 2003Dissertation note: The present study was designed to estimate the efficacy of different locally prepared anticoccidial vaccines against E. tenella infections i.e. irradiated non-sporulated vaccine and irradiated sporulated E. tenella vaccine, herbal anticoccidial (Coxigon) and cemical anlicoccidial (Suipha Diverdine). For estimation of the efficacy of the said anticoccidials, OPG counts, weight gain, FCR and mortality records were compared amongst the control and test groups.
A total of 150 day-old broilers were equally divided into six different groups on day 3 namely; A, B, C, D, E and F. Each group comprised of 25 chicks. ND vaccine was administered on day 1 and 21 of age to the chicks of all groups. rIhe test groups were infected through contamination of the bedding of the chicks by spraying sporulated oocysts between day 12 and 17 of the experinient. Group A acted as non-infected control, .13 as infected control, C was infected but vaccinated with irradiated non-sporulated E. tenella vaccine on day 3 and 10 @ 1000 oocysls per bird. Group D was infected and treated with Coxigon (herbal medicine), E was infected but treated with Coxidal (Sulpha diverdine). Similarly group F' was infected and vaccinated with irradiated but sporulated oocyst on day 3 and 10 @ 1000 oocysts per bird. To access the comparative efficucies of different groups mean OPG, mean weight gains and mean FCR of control and test groups were compared. The OPG counts were conducted on each 4th day from day 18th to 42. The results indicated that the first observation of OPG counts (day-18) showed heavy infection in all control (infected) and test groups. In group 13, 264523 oocysts were observed on day 18 which gradually reduced to 15773 on day 42. Group C (vaccinated with non sporulated, irradiated oocysts) showed 14214 OPG counts, which sharply declined on each observation and showed 89.00 QPG and day 38 followed by '0, by the end of the experiment, showing significaht difference from the control (1? <0.05). Group D (Coxigon) and groap E (Coxidar) showed gradual decline like infected control group and
735 OPG counts were observed in group E even on day 42. In group F the first observation showed 150428 OPG counts, which abruptly declined to 119 on day 38 followed by '0' on day 42. So group C and F' showed comparable and the best results as compared to groups D and E.
Weight gains were almost equal in all the test and control groups by day 14. The difference appeared on third observation (on day 21) between infected control and other groups. At the end of the experiment members of groups A, C, D, E and F gained 31%, 14%, 11.83%, 10.92% and 12% greater weights than group B. rphe FOR in ranked order was calculated in groups A, F, C, D, E and B, showing the cfCicacy of irradiated - sporulated vaccine. Mortalities occurred in groups B, C, D, E and F as 5, 2, 1, 4 and 1, respectively.
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100.
Identification And Effect Of Gigantocotyle Expalnatum On The Haematology And The Liver Enzymes Of Buffaloes
by Rizwan Muhammad Samee | Mr.Kamran Ashraf | Dr.Haji Ahmad Hashmi | Mr.Muhammad | Faculty of Veterinary Sciences.
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Publisher: 2003Dissertation note: Amphistomosis is a wide spread disease of buffaloes,cattle,sheep and goats.acute disease has been reported in young animals while older animals arecapable of withstanding massive infection.Heavy infection of amphistomes both in rumen and liver are mostly insidious and generally do not cause serious damage t the host. In the redent study, ist has bveen concluded that due to its mild subl\clinical nature, deworming for the control of biliary amphistomes is n\done very rarely and large animals, once infected carry this trematode throughout rest of thi\eir life. The prevalence of the Gigntocotyle explanatutum detercted in Lahore district was 42.1%. Statistically ono-signifcant difference in prevalence was observed during different months of study which may be attributed to the lack of dewormingfor the control of biliary amphistomosisis done very rarely and large animals, once infected carrys this infection throughout there lives. The prevalence of the Gigantocotyle explanatum detected in Lahore Ditrict was 42.1%.Statistically non significant differences in prevalence was observed during different months of study which may be attributed to the lack of deworming practices in animals.High prevalence in buffaloes might be due to wallowing habit and grazing grasses and weeds.The infected buffaloes had significant decrease in Hb concentration (30.43%) ,and PCV (29.2%).However,there was no significant increase in TLC (1.93%).No significant decrease in neutrophils(3.71%),monocytes(13.41%),lymphocytes(1.50%)and basophils(13.33%) counts was observed. There was significant increase in eophilic counts(52.74%).The ALT and AST were also estimated in infected andhealthy buffaloes.The statistical analysis showed significant increase in AST (28.5%) and non significant increase in ALT (13.0%).
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