Effect Of Various Levels Of Probiotics(Lactobacillus Acidophilus And Bifidobacterium Bifidum) On Physicochemical, Microbiological And Sensory Characteristics Of Ice Cream
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Publisher: 2010 Dissertation note: Ice-cream is a frozen mixture of combination of components, such as milk, sweeteners, stabilizers, emulsifiers and flavoring agents. Ice-creams are food products which show excellent potential for delivering probiotics to consumer. Probiotics are basically health promoting gut friendly bacteria. Minimum viable quantity of probiotics which is beneficial for human beings is 106 or 107 cfu/g. Benefits are strain specific and cannot be extrapolated. Keeping in view the importance of probiotics the present research study was planned to determine the influence of various levels of probiotics (Lactobacillus acidophilus and Bifidobacterium bifidum) on physicochemical and sensory characteristics of ice cream, assessing the viability of probiotics at different storage periods and to give innovative and value added product to commercial ice cream manufacturers. In order to carry out the study, Freeze dried cultures of two probiotic bacteria i.e., Lactobacilus acidophilus (DVS LA-5 Probio-Tec®) and Bifidobacterium bifidum (DVS BB-12® Probio-Tec®) were obtained from Chr. Hansen (Hørsholm Denmark). The probiotic cultures were stored at - 18? C in freezer.
These two probiotic strains were inoculated alone and in combination in ice cream mix at three different inclusion levels. Hence there were total nine experimental treatments. A regular or control ice cream i.e., without any probiotic culture was also used along with treatments. Probiotic ice cream preparation and physical tests were performed at walls Ice Cream factory, Lahore, Pakistan. Chemical analysis were performed at department of Food and Nutrition laboratory, whereas, Microbiological tests were conducted at department of Microbiology, University of Veterinary and Animal Sciences, Lahore.
Probiotic ice cream was manufactured in the pilot plant of the research and development department, Unilever Walls Ice Cream factory Lahore. Ice cream mix was prepared by following standard procedure. After mixing, homogenization and pasteurization of ice cream mix, it was cooled to 41°C and was divided into ten equal parts. Probiotics cultures alone and in combination, according to experimental design, were added in ice cream mix. Then it was fermented for two hours at 41°C. Then mix was aged at 4°C for a period of 2 hours followed by freezing and hardening. Ice cream samples were packed in one liter plastic tubs and were stored at - 18°C in freezer.
Ice cream samples were analyzed after every fifteen days interval during storage period of three months. Physicochemical tests including overrun, viscosity, melting resistance, pH, acidity, fat, protein, total solids were performed by following the methods of AOAC (2000). As far as results of physicochemical tests are concerned, it was observed that both Lactobacillus acidophilus and Bifidobacterium bifidum are able to produce acid in ice cream. Acidity tended to increase as a result of increase in the number of bacteria. Highest pH was observed in control (R) sample, whereas, lowest pH was observed for samples having mixed culture. pH tended to decrease throughout storage. Lactobacillus acidophilus found more acid producing than Bifidobacterium bifidum. Ice cream samples containing high levels of probiotic bacteria showed decreased value of overrun. Whereas no effect was found in case of viscosity. Samples with increased level of bacteria showed significantly less melting resistance. Also, melting resistance tended to decrease significantly with increase in storage. Total solids and fat contents remained constant throughout storage and effect of treatments was statistically non significant. In case of protein contents, a positive correlation was observed .Increase in level of bacteria , increased the protein contents of ice cream, but it remained constant throughout the storage.
Viable probiotic bacteria were enumerated by using the technique of spread plate method by using RCA media. In present study, cell count of viable bacteria, after fermentation, showed increase number of colonies. Samples inoculated with B3 were excellent in regard of exhibiting probiotic property followed by treatments B2 and A3. Treatments in combination i.e., C1, C2 and C3 showed results close to treatments having single cultur , which might be due to nutrients competency with each other. Furthermore, it was quite difficult to count colonies in mixed culture. Ice cream samples prepared from mixed culture were more acidic in taste and flavour and were least liked by sensory evaluators.
Probiotic ice cream was subjected for sensory evaluation by five panel of judges. They rated ice cream by using nine point hedonic scale. Color of ice cream did not show any change throughout storage and was not affected by various levels of probiotics. No surface spots were found in any treatment at any storage period. Due to acid producing nature of probiotics, slight acidic changes were observed in thickness, flavour and taste of ice cream. However, overall acceptability for all probiotic ice cream was good. The data were analyzed according to analysis of variance technique under factorial arrangement. Significance of means was compared by using Duncan's multiple range test.
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Study On Chemical Composition Of Nili Ravi Buffalo Milk
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Publisher: 2010 Dissertation note: Milk is a complete food and contains all the nutritional components in balanced form. It helps to meet important nutritional needs of the human body. Vitamins, minerals, sugar, Fat, and protein are present in milk. Among the established breeds of buffalo in Pakistan. the Nili-Ravi breed originated in the valleys of Sutlej and Ravi rivers is dominant in the Punjab province. The purpose of the study is to determine the chemical composition and fatty acid profile of buffalo milk.
The milk animals, Buffaloes were divided into 3 groups on the basis of lactation period i.e early, middle and late lactation. Five animals were present in each group. Six milk samples. 3 in the morning and 3 in the evening from each animal was collected. A total of 90 milk samples were obtained for analysis. Milk in bottles of 500 ml capacity was transported to lab. [he samples were analyzed in Livestock Production Research Laboratory, Livestock Production Research Institute Bahadurnagar (Okara). Temperature, Density, Conductivity and freezing point was determined by using Milkoscan.
Buffalo samples were analyzed for proximate analysis, parameters like fat, crude protein, lactose, ash, solids not fat, total solids, pH and acidity were determined. Fatty acid profile of buffalo milk was determined by the method of AOAC (2000). Milk fat content (%)was Ihund to be significantly (p<0.05) lower in the early (6.52) and mid lactation (6.61) stages than the late lactation stage (6.85). The content of TS (%) was found to be significantly (p<O.05) higher in the late lactation (14.62 and 15.45) stage than the early (12.94 and 13.65) and mid lactation (13.76 and 14.14) stages. In contrast, the content of total protein (4.31 to 4.78%), SNF (X.4() to8.70%), lactose (4.36 to 4.74%), and ash (0.l8to 0.19%) did not vary significantly among the different lactation stages.
Fatty acids profile also checked from Cl to C20. The values of Butyric acid, Caproic acid. Myristic acid, Stearic acid, and Oleic acid showed significant increase towards end of lactation. 'lhcse values of fatty acids also revealed that there is significant difference among the animals and between the lactation stages of Nih- Ravi buffalo milk. Results showed that Capric acid, Laurie acid, Palmitic acid, Palmitoleic acid, Linoleic and Linolenic acid decreased in early to middle and increased towards end of lactation.
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Improving Nutritional Value And Acceptability Of Dairy Products With Lower Contents Of Saturated Fatty
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Publisher: 2013 Dissertation note: Abstract
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The Effect Of Date Palm Fruit (Phoenix Dactylifera L.) Extract On Oxidative Stabilization Of Butter At Ambient
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Publisher: 2013 Dissertation note: This investigation aimed to explore the antioxidant potential of date fruit extract using butter as an oxidation substrate on the basis of certain instrumental, orthodox analytical techniques and sensory characteristics. The objectives of this study were to characterize the date extract for polyphenolic compounds, free radical scavenging activity and inhibition of nitric oxide macromolecules and to study the effect of various concentrations of the date palm extract on oxidative stability and sensory characteristics.
The butter was added 200, 400 and 600-ppm respectively date palm extract and compared with a control, stored at refrigeration and ambient temperature during winter months for 90-days at the interval of 30-days. Peroxide value, anisidine value, iodine value, conjugated dines, totox value, free fatty acids and sensory evaluation were performed in the fresh and three month stored butters. Sensory evaluation of supplemented butter added with various concentrations of date palm extract stored revealed the effect of treatments, storage period and their interaction to be significant. Taste, Color and overall acceptability score was non-significant up to 30-days of storage. After this sensory score started to deteriorate and went on decreasing the rest of storage period. The decline in taste score was not due to the addition of date palm extract rather it was due to the generation of peroxides and other oxidation products. Oxidation products and peroxides are found to be in direct relation with temperature; with rise in temperature the decline in sensory evaluations was more pronounced. This connection between taste score and peroxide value was highly correlated (R= 0.9982).
The results of triplicate treatments and triplicate analysis (n=3x3; ±SD n=3x3) was analyzed by using one way analysis of variance techniques (ANOVA) to find out the effect of the date palm extract to the storage conditions. Differences among treatments were compared by Duncan Multiple Range Test.
Supplementation of butters with date palm fruit extract did not revealed any significant effect on the inhibition of free fatty acids (P>0.05). The free fatty acid content of fresh and 90 days stored treatments and control were at par with each other. The peroxide value of all the treatments and control increased during the storage period but to varying degree. The addition of date palm extract revealed a significant effect on inhibition of peroxide value (P<0.05). The peroxide value of fresh supplemented, control and 90 days stored samples showed a significant difference. The difference in control and treatments were in order of To > T 1 > T2 > T3 in 90-days stored experimental samples and control. T3 revealed a maximum resistance towards the increase of peroxide value during storage period of 90-days over the control at refrigeration temperature. It is evident from the result that the addition of date palm extract significantly inhibited the auto-oxidation phenomenon in butter stored at room temperature, however the concentration of oxidation products were higher as compared to butter stored at refrigeration temperature. The iodine value decreased during the storage period in control experimental samples throughout the storage period of 90-days but to varying extents at refrigeration temperature. Control (T0) showed highest decrease in iodine value while T3 showed least change in iodine value during the storage period.
The anisidine value of control and treatments stored at refrigeration temperature were in the order T0 > T1 > T2 > T3. Control revealed significant increase in anisidine value as compared to treatments. After 90 days of storage period the extent of secondary oxidation products in control and T3 was 22.24 and 8.35 at refrigeration temperature respectively. The classical rise of anisidine value was observed in all the treatments to varying degree, supplementation of natural antioxidant offered a great deal of resistance towards the rise of anisidine value. Supplementation of butter with date palm fruit extract revealed significant effect on the generation of oxidation products. Conjugated dienes for control and all the treatments increased throughout the storage time slowly and steadily.
Analysis of variance revealed that the effect of treatments, storage period and their interaction was significant. Taste, Color and overall acceptability score was non-significant up to 30-days of storage. After this sensory score started to deteriorate and went on decreasing the rest of storage period. The results indicate that ethanolic date palm extract at 600-ppm concentration can be used as natural antioxidant source for the long term storage of butter at ambient temperature during winter months. The outcome of this study will help the food industry for preservation of fats and oils.
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Photo-Oxidation Of Pasteurized Milk In Polyethylene Pouch Packs
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Publisher: 2014 Dissertation note: Abstract
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Fractions Of Milk Fat On Lipolysis In Cheddar Cheese
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Publisher: 2014 Dissertation note: Lipids are heterogeneous group of biological compounds, soluble in fat and insoluble in water. Oils and fats mainly comprise of triglycerides and has great importance in Food systems. Fats and oils are completed through ester of three fatty acids and a glycerol molecule (Stolyhmo, 2007; McClement and Decker, 2010; Nichols et al. 2011). Fats have a primary importance for the texture of Foods (Rao, 2003).
Fat has more energy values as compared to other Foods nutrients (Wu et al. 2013), presently, one of the major developments is declining the cholesterol contents and fat in the Foodstuffs (Ma and Boye, 2013). Sensory attributes like appearance, flavor, texture and physicochemical properties of the Foods depend upon the fat because it is very important for the Foodstuffs improvement. For these properties, there should be practical strategies which can reduce fat contents only in the Foodstuff (Wu et al. 2013) and should not effect on nutritious and sensory properties (Boff et al. 2013).
Milk fat can be transformed into various fractions such as very high melting fraction (>50°C), high melting fraction (35-50°C), middle melting fraction (25-35°C), low melting fraction (10-25°C) and very low melting fraction (<10°C). Low melting fraction (<15°C) has strong butter flavor and can be incorporated into milk powder to improve functionality. It can be used in confectionery products and can increase spread ability of butter at low melting temperature (Gunstone, 2001).
It has been investigated that melting point of butter oil decreased with C18:2 and increased with C16:0 and C14:0 fatty acids. It has also been investigated that melting point is negatively correlated to C40, C38, C30 and C28 tri-acylglyceride while positively correlated with C48, C46 and C44 tri-acylglycerides (Ortiz-Gonzalez et al. 2007).
The anhydrous milk fat has a higher fraction of low melting tri-acylglycerids due to more unsaturated fatty acids (Smet et al. 2010).
Fatty acids are composed of carbon and hydrogen atoms having one carboxyl (COOH) group at one side of the chain (Ghatak and Bandyopadhyay, 2007). Fatty acids are divided into short, middle, long chain fatty acids and also in saturated and unsaturated fatty acids. Unsaturated fatty acids are further categorized as monounsaturated, poly unsaturated fatty acids and saturated fatty acids. Almost 65-68% saturated fatty acids of milk fat possess many health concerns (Richmond, 2007). More than 4 hundred types of fatty acids are present in milk fat of different bovine breeds. Plasma cholesterol and incidence of coronary heart disease is increased by medium chain saturated fatty acids of milk fat lauric acid (C12:0), myristic acid (C14:0) and palmitic acid (C16:0) (Jensen, 2002). Fatty acid composition of diet has a great impact on health. Considerable attention has been given on the saturated fatty acids present in diet (Astrup et al. 2011). Nutritionists recommend that saturated fatty acids should be replaced by unsaturated fatty acids to decrease the incidence of cardiovascular disease (Erkkila et al. 2008).
Medium chain fatty acids C12:0, C14:0 and C16:0 are atherogenic (Parodi, 2004; Kris-Etherton and Innis, 2007). The coronary heart diseases and atherogenic fatty acids are highly correlated (Moss and Reed, 2003; MacRae et al. 2005; Mensink, 2006). As compared to other lipids sources dairy products are well known to increase the cardiovascular diseases in human beings, because dairy products contain lower proportion of unsaturated fatty acids and higher proportion of saturated fatty acids especially palmitic and myristic fatty acids (Sacks and Katan, 2002). Dairy Foodstuffs are the sources of dietary cholesterol which increases the serum cholesterol (Collins et al. 2003).
Modification in milk fat can be carried out by chemical and enzymatic interesterification (Pal et al. 2000). Physical modiﬁcation (fractionating of bovine milk fat into different fractions) of milk fat can be carried out by fractionation (Ali and Dimick, 1994). Commercial dairy industries commonly use dry fractionations to improve the texture and flavour properties of dairy products (Grall and Hartel, 1992). Nadeem et al. (2013) found in a study that long chain and short-chain fatty acids can be increased by fractionation of milk fat. Fractionation improves the functional properties of milk fat. Low melting fractions of milk fat can be used in a wide range of functional dairy products.
Cheese is worldwide extensively used dairy product. Researchers have tried to improve the unsaturated fatty acid composition of milk fat by blending with vegetable oil but the use of vegetable oils in cheese has a negative impact on texture, functional properties, excessive lipolysis and flavor characteristics of cheese (Wijesundera and Watkins, 2000;Yli-Jokipii et al. 2001; Dinkci et al. 2011). There are many factors which affect the texture and rheological properties of the cheese. These factors also effect the appearance, functional properties of the cheese and also effect the flavor of the cheese which are very important for consumer. On the base of texture varieties of cheeses are differentiated. Important property for the determination of differences in the cheeses is texture as compared to other flavor and taste property (Wendin et al. 2000)
Free fatty acids of cheddar cheese produced in the result of lipolysis are the precursors of flavor compounds (Smit et al. 2002; McSweeny, 2004). The suitability of low melting fractions of milk fat as a substrate in the manufacturing of cheddar cheese has not been previously investigated.
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Chemical Characteristics Of Trans Free Margarine Enriched With Omega Fatty Acids Through Chia (Salvia Hispanica L.) Oil
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Publisher: 2015 Dissertation note: Omega fatty acids has been related with low cholesterol level in blood, reduce the blood pressure, decreases the risk of heart attacks. Omega-3 PUFA is anti-carcinogenic, anti-atherogenic, anti-lipogenic, prevent the hypertension. Margarine was prepared by blending milk fat 70%, palm oil different concentration 30%, 27.5%, 25%, 22.5% and 20% T0 to T4 respectively .Chia seed oil in various proportions 0%, 2.5%, 5%, 7.5% and 10% T0 to T4 respectively. Milk fat, Palm oil and chia Seed oil was characterized for free fatty acids, moisture content, saponification value, iodine value, refractive index. Solid fat index was determined at 0, 10, 20, 30 and 40˚C by the dilatometric method, melting point was determined by open capillary tube technique.Color, peroxide value, anisidine value, conjugated dienes and trienes was determined. Fatty acids composition of margarines were determined by the transformation into fatty acid methyl esters. Margarine was stored at -6±1oC, for 60-days, iodine value, peroxide anisidine values, free fatty acids, conjugated dienes and trienes was determined. Induction period of margarine was determined by oxidizing the samples under a steady stream of oxygen (20L/hour) at 120oC, on a Rancimat. Sensory evaluation was performed by a panel of ten trained panel of judges, samples was evaluated for color, smell, taste and overall acceptability on a 9-point Hedonic scale.All treatments were replicated three times, every sample was analyzed three times and data were expressed as Mean ± SD. The data was analyze by one way and two way analysis of variance technique, the difference among the treatments was made by Duncan Multiple Range Test
Free fatty acids increased in all the treatments during the entire storage period from 0 day to 60 days, the content of fatty acid during storage period depend upon the degree of unsaturation. . Peroxide value increased in trans free margarine enriched with omega fatty acids through chia oil the during the storage of margarine. The rise in peroxide value at all the
determination frequencies was in the order of T4> T3> T2> T1> control. Iodine value increases in trans free margarine enriched with omega fatty acids through chia oil during the storage time. The decline in iodine value of all the treatments and control was in the order of T4> T3 > T2 > T1 > control. The treatment having more unsaturated fatty acids, underwent more oxidation and yielded the higher extents of oxidation products. Anisidine value of all the treatments and control increased throughout the storage period, all the determination frequencies revealed an increasing trend, however, the rise in magnitude of oxidation products was different in all the treatments and control.Values of conjugated dienes and trienes numerically increased during the storage period, all the measurement intervals revealed an increasing trend, however, the rise in extent of oxidation products was mainly dependent upon the fatty acid composition and treatments having higher concentration of unsaturated fatty acids suffered more oxidation.Addition of chia oil in margarine did not have any impact on moisture content of margarine, moisture content of all the treatments and control was not different from each other (P>0.05). Non-significant changes melting point during storage time in margarine. Color of all the experimental margarines and control were not different from each other (P>0.05). Saponification value non-significant in treatments during storage time from 0 day to 60 days. Analysis of variance indicated that treatments had significant effect on SFI, whereas, the effect of storage and the interaction between treatments and storage was non-significant. The content of C18:3 (omega fatty acid) in margarine is significantly increased from T1 to T4 due to chia oil because it contain 68 % alpha linolenic acid.
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Enhancement Of Omega Fatty Acids In Margarine Through Chia (Salvia Hispanica L.) Oil
Material type: Book ; Literary form:
Publisher: 2016 Dissertation note: The higher incidents of deaths from cardiovascular diseases have led to the identification
and modification of risk factors for atherogenesis; it is regarded as the number one reason of
deaths in developed and underdeveloped countries. Chia contains about 40% edible oil, with
fairly high content of unsaturated fatty acids, oil contains about 60% omega-3 fatty acids on
weight basis. Margarine will be prepared from palm oil, palm kernel oil, butter and chia oil
blends by varying the concentration from 5% to 20%.
Margarine was prepared by blending butter, palm oil and Chia seed oil in various
proportions. Market margarine (Blue Band) was used as a positive control. Butter, Palm oil and
Chia Seed oil was characterized for free fatty acids, moisture content, saponification value,
unsaponifiable matter, iodine value, refractive index. Solid fat index was determined at 0, 10,
20, 30 and 40˚C by the dilatometric method, melting point was determined by open capillary
tube technique. Colour, free fatty acids, iodine value, peroxide value, anisidine value,
conjugated dienes and trienes was determined. Fatty acids composition of margarines was
determined by the transformation into fatty acid methyl esters. Margarine was stored at 6±1oC,
for 2 months, iodine value, peroxide anisidine values, free fatty acids, conjugated dienes and
trienes was determined. Sensory evaluation was performed by a panel of ten trained panel of
judges, samples was evaluated for color, smell, taste and overall acceptability on a 9-point
Peroxide value of 60 days old control and T4 (12% chia oil) were 7.06 and 12.10
(MeqO2/kg). After 60 days of storage period, peroxide value was in the order of T4> T3> T2>
T1> control. Free fatty acids increased with the storage period in all the treatments. They are
also increasing with the treatments having higher concentration of chia oil. At 60 day, control
and T4 are 0.16 and 0.19% respectively. Anisidine value trend is considerably accelerating
along the treatments as well as with the storage time. At 60 day, control and T4 is 24.42 and
44.78 respectively. Conjugated Dienes have the increasing trend throughout the treatments.
They also have higher values from 0 to 60 days of time period. At 6o day, the control and T4
are 2.47 and 3.34 respectively. Conjugated Trienes of all the samples increased throughout the
storage period of 60 days. At 60 day, the control and T4 are 2.60 and 3.28 respectively. Iodine
value is in the decreasing trend throughout the storage period and the treatments. They are in the
order of T0>T1>T2>T3>T4. Fat content of all the treatments and the control went on decreasing
throughout the storage period of 60 days. At 60 day, control and T4 were 68.61 and 90.91
respectively. The order of the fat content in all the treatments were T0>T1>T2>T3>T4. Moisture
content of all the treatments and the control went on decreasing throughout the storage period of
60 days. At 0 day, Moisture content of all the treatments were virtually the same. At 60 day,
control and T4 were 27.52 and 7.64 respectively. Salt content of all the treatments at the end of
the storage period were increased. At 60 day, control and T4 were 4.18 and 1.42 respectively.
The taste score of all the treatments along the treatments having different concentrations of
unsaturated fatty acids and also along with the storage period of time is decreased. The taste
score of the control and T4 were 8.06 and 6.90 respectively. The order of the taste score is in the
descending order of T0>T1>T2>T3>T4. Results of this evidenced that concentration of omega
fatty acids can be enhanced in margarine through chia oil with acceptable sensory
characteristics. Major changes in fatty acid composition were observed around C18:2 and C18:3
(omega 6 and 3). Concentration of trans fatty acids in control, T1, T2, T3 and T4 was 24.75%,
6.67%, 5.49%, 4.71% and 3.19%.
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