51.
Haematological And Serum Protein'S Values Of Young Hourses Affected With Strangles In Sargodha
by Zahid Hassin Abid | Manzoor ahmed Basra | Muhammed | Zafar Iqbal Chaudry | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1992Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0243,T] (1).
52.
A Study On The Patholgoy Of Internal Genital Organs Of Cows
by Tariq, M | Manzoor Ahmed Basra | M.sarwer khan | Zafar iqbal Chaudry | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1992Dissertation note: Genital abnormalities inflict heavy economic losses. There were many abnormalities which do not Interfere with normal health of living animals but causes Infertility and sterility. Some of these abnormalities can only be diagnosed If the animal is subjected to postmortum examination. For this purpose three hundred and seven Internal genitalia of non descript cows were collected from abattoir, during the months of August, September and October 1991. The samples thus collected were catagorised Into three age groups I.e. group A (Less than 3 years), group B ( 3 to 7 years) and group C (7 Years and above). The specimens from each carcase were collected, labelled and brought to the pathology laboratory for gross and histopathological studies.
Out of 307 genitalia, the distribution of samples was 74 In group A, 138 in group B and 95 in group C.
In different age groups, the macroscopic lesions observed from the ovaries and bursae consisted of ovarian hypoplasia in 1.62%, inactive ovary In 1.30%, Ovarlo-bursal adhesion in 3.90%, follicular cyst in 2.60%, cystic corpus luteum in 1.95% and Par-ovarian cyst in 0.65% genitalIa. The macroscopic lesions observed In fallopian tubes were salpingitis in 0.65% and hydrosalplnx in 0.65% cases. The gross lesions of uteri were obstruction of one horn In 0.32%, endometritls In 2.93%, pyometra in 2.28%, mucometra in 1.62% and mummified foetus in 0.65% cases. The gross abnormalities observed from the cervix consisted of cervicitis In 2.93%, mucocervix in 1.62%, cervIcal dialation In 0.65% and twisted cervix in 0.65% animals. Above mentioned acroscoplc lesions in different parts of genitalia was confirmed microscopically.
Availability: Items available for loan: UVAS Library [Call number: 0244,T] (1).
53.
Histopathological Studies Of Experimentaly Produced Disease Of Ranikhet In Broilers And Layers
by Sait ud Din Jahagir Abkar | Manzoor Ahmed Basra | Tufail Muhammed Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
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not fiction
Publisher: 1985Dissertation note: A total number of sixty four, day old chicks of which 32 broilers and 32 layers were used. The incubation period was increased with age while the mortality rate was reduced with increase of age.
Macroscopic examination of proventriculus, intestine, heart and brain revealed haemorrhages, in addition to that liver, spleen and kidneys showed necrotic foci. The nasal passages were full of catarrhal exudates.
Microscopic appearances of trachea, lungs, proventriculus, liver, heart, kidneys and spleen showed lymphocytic infiltration. The blood vessels were dilated. The necrotic changes were prominant in all the organs.
Availability: Items available for loan: UVAS Library [Call number: 0260,T] (1).
54.
Studies On Histopathology Of Brain And Salivary Glands Of Dogs Suspected For Rabies
by Shakil Akhtar Khan | Tufail Muhammed Khan | Ata-Ur-Rehman | Manzoor Ahmed Basra | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1986Dissertation note: Rabies is a fatal viral disease of all warm blooded animals, including man. Because of its highly fatal nature it is the most notorious and dreaded disease in dogs. The disease in man is characterized by an emotional and neurological disorders with relfex excitability, paralysis and death. The infectious agent of rabies is a neurotropic member of the family Rhabdoviridae found in the nerve tissue, tears, saliva, salivary glands and rarely in other body fluids.
A total of thirty stray dogs and thirty dogs suspected for rabies were included in the present work. From the both groups, the impression smear5were prepared (of hippocampus, cerebellum and mandibular salivary gland) for detection of Negri bodies. Biological test in mice and histopathological changes of bippocampus, cerebellum and mandibular salivary glauds were noted. Out of 30 stray dogs the impression smears of three hippocampus and one cerebellum were doubtful for Negri bodies and all the mandibular salivary glands were negative, for Negri bodies. The biological test in mice was negative for rabies in all stray dogs. The impression smears from thirty dogs suspected for rabies, showed Negri bodies in twelve hippocampus, seven cerebellum and all the mandibular salivary glands were found negative for Negri bodies. Seventeen dogs out of 30 dogs were biological positive for rabies. Histopathological changes were observed in fourteen hippocampus, fourteen cerebellum and ten mandibular salivary glands.
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55.
The Effects Of Coccidiostatic Sulfa Drugs On The Blood Of Chicken
by Akmir Ghani | tufail muhammed Khan | Manzoor Ahmed Basra | Mubasher | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1985Dissertation note: Amongst the various poultry diseases coccidiosis is of paramount importance. Many drugs are commonly used against coccidiosis for prophylactic and therapeutic purposes which are found to be toxic. This study was conducted to see the adverse effect of coccidiostatic sulfa drugs Polycox, Darvisul and. Esb3 on the blood components, liver and kidneys of the chicks. These drugs were given to 70 broiler chicks at 4 weeks of age at therapeutic dose and higher level for 3-2-3 days. Their blood was collected on the 9th day post-medication to see the adverse effects on hematological values.
The coccidiostatic sulfa drugs medication produced severe anemia characterized by decrease of erythrocytic count, leukocytic count, hemoglobin contents and hematocrit values. Heterophils, lymphocytes and monocytes decreased significantly. The coccidiostatic sulfa drugs also effected the maturation of the cells in the bone marrow and produced a significant decrease of immature stages of erythrocytic,granulocytic and thrombocytic series. Erytliroblasts, polychromatic erythrocytes, thromboblast, immature and mature thrombocytes and granuloblasts decreased significantly by coccidiostatic su-fa drugs. The bone marrow smears were chiefly composed of erythrocytes and lymphocytes. The bone marrow was pale and fatty in appearance. This study has shown that changes in the blood picture are airectly related to the maturation of the cells in the bone marrow.
On gross examination liver and kidneys showed haemorrhages and necrosis, while microscopic examination revealed degenerative changes, necrosis, haemorrhages and infiltration of mononuclear cells which were prominent. It was evident that increase in the dose level of coccidiostatic sulfa drugs increased the severity of adverse effects. Esb3 produced less toxic effect than Polycox and Darvisul (containing Suiphaquinoxaline and Diaveridine).
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56.
Study On The Normal Haematology And Biochemistry Of Blood Of Buffaloes
by Waqar Sarwar Gillani, Syed | Muhammed Irfan | Tufail Muhammed Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
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not fiction
Publisher: 1984Dissertation note: Two hundred clinically normal and healthy buffoloes were studied for different haematological and biochoraical parameters. The animals from birth to thirteen years of age were included in the study.
i) A significant variation in the values of haemoglobin, total erythrocyte count and packed cell volume was seen between animals below 1 2 months of age and above 1 2 months of age.
2) Animals below 12 months of age showed significantly higher haemoglobin contents, total erythracyte count and haematocrit values.
3) Animals below 12 months possessed significantly higher blood glucose level.
4) The cholesterol level in lactating animals was higher compared to non lactating animals.
5) The average values for buffaloes below 12 months of age were as follows: Haemoglobin 12.0 ± 2.H prcent
Total erythroeyte count 8.84 + 1 .85 millions/cmm
packed cell volume 39.7 ± 9.7 per cent
6) The average haematological values for buffaloes above 12 months of age were as under
Haemoglobin
Total erythrocyte count
Packed cell volume
Mean cell volume
Mean cell haemoglobin
Mean cell haemoglobin concentration
Total leucocyte count Absolute neutrophil
11.6 ± 2.8 go per cent
7.48 ± 1 .98 millions/cram
35.35 ± 6.35 per cent
47.92 +
15.95 ±
30.42 ± 4.02 per cent
11641 . 5 ± 1 239 .5 thousands/cm
4339.5 + 540.5 per cubic-milimetor
Absolute lymphocyte
Absolute monocyte
Absolute eosinophil
Absolute basophil 6388.5 ± 667.5/corn
556.5 ± 107.5/cmm
464i-97/emm
Nil
The average biochemical values for buffaloes above 12 months of age were as follows:-
Glucose
Urea
Cholesterol
Inorganic phosphorous Calcium
Calcium
Potassium
Sodium
Choloride 59.7 ± 13.9 mg per cent
33.75 ± 11.25 mg per cent
280.5 ±25.5 mg per cent
5.84 ± 1 .84 rng per cent
9.69 ± 2.91 mg per cent
4.6 ± 1.0 miliequivalent/lit.
136.5 ± 8.5 miliequivalunt/lit
105.5 ± 9.5 miliequivalent/lit
The blood glucose for anirals below 12 months of age was 71.25 ± 14.35 mg per cent.
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57.
A Study On The Pathology Of Internal Genital Organs Of Buffalo Heifer
by Adil Pervaiz Anwar Khan | Manzoor Ahmed Basra | Tufail Muhammed Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1986Dissertation note: The buffalo (Bulus bubalis) is a triple purpose animal. It provides milk and meat and is extensively used for work in certain parts of the world. It is a good source of fat, protein, vitamins and minerals for human diet. The buffalo milk with its higher fat content is considered by most of its consumero as superior to cow milk.
Gross lsions of the ovaries, oviducts, uteri and cervices, and histopathological changes of the morbid tissues of the ovaries, oviducts, uteri and cervices were studied on the internal genitalia of 150 buffalo heifers. In addition E.coli was isolated and identified from the animals routinely slaughtered at the slaughter house, Lahore, during the months of February and March, 1986. The specimens from each heifer's carcass were collected, labelled, packed in a separate polythene bag and brought to the Pathology laboratory, College of Veterinary Sciences, Lahore in a box containing ice.
The macroscopic lesions recorded from the ovaries and bursae consisted of cystic follicles in 8 (5.3%), par-ovarian cysts in 9 (6%), hypoplasia in 7(4%), ovaro-bursal adhesions in 8(5.3%) cases. The gross lesions of fallopian tubes were hydrosalpinx in 6(4%) cases. The gross lesions of uteri were mucometra 5(3.33%) and haemorrhages were also in 5(3.33%) cases. The cervices revealed no pathological abnormality. Histopathlogical changes confirmed the above mentioned gross pathological changes.
Escherichia coli was isolated from the uterine horns of 31 out of 150 buffalo-heifers. For the isolation of E.coli uterine material was cultured in nutrient broth tubes, - incubated at 37°C overnight. A smear was prepared from each incubated tube, stained and examined for the presence of Gramnegative organisms. The Nutrient broth containing gram-negative organisms were further inoculated on MacConkey's agar media plates and incubated for 24 hours at 37°C. The characteristic circular, opaque, glistening and rosepink colonies of E.coli on this medium were recorded. Biochemical properties of isolated E.coli isolates were studied. The pathogenicity of these isolates was tested in Swiss albino mice intraperito- nially. No pathogenic isolate of E.coli was found out of 31 E.coli isolates and E.coli could not be isolated from the heart blood of these mice when they were killed after eight days.
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58.
Pathological Changes Caused By Escherichia Coli In Young And Adult
by Tufail Muhammad Khan | Manzoor Ahmed | Altaf Hussain Sheikh | Muhammed | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1977Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0270,T] (1).
59.
A Study On The Effects Of Organos Phosphate (Insecticide) On Various Organs And Blood Parameters Of Broiler Chickens
by Shahzada Khurram Ashraf | Manzoor Ahmed Basra | Muhammed | Zafar Iqbal Chaudry | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1992Dissertation note: The investigation was conducted to study the effects of organophosphate insecticide Trich- lorf on (Ditrifon) on the hematology and liver, kidneys, heart and spleen of the broiler chicken. One hundred and twenty day old broiler chicks were reared upto 3 weeks of age, then they were randomly divided into four groups i.e. A, 8, C and D containing 30 chicks in each group. Group A was maintained on afeed containing Ditrifon at dose rate of 400 mg/kg in feed. Group, B was maintained on a feed containing Ditrifon at dose rate of 200 mg/kg in feed. Group C was maintained on a feed containing Ditrifon at dose rate of 100 mg/kg in feed. Group D was kept as control. All the said groups were maintained on said feed for 7 days. The weights of the birds from each group were taken before treatment with Ditrifcon. All the groups were kept under close observation to record signs and symptoms of Ditrifon toxicity upto the end of 4 weeks and 5 weeks of age 15 chicks from each group were slaughtered for the hasmatology (PCV, Hb concen- tration, TEC and TLC) and histopathological studies (liver, kidneys, heart and spleen). The weight of the chicks from each group were taken and recorded before slaughtering.
There was a significant decrease in weight in groups A,E4 and C as compared to control group D.
Antemortem symptoms were recorded throughout the experiment and observed that feed intake of the treated groups was decreased, growth was retarded significantly. The treated birds of Group A and Group B showed dyspnoea, tremors, exhaustion, incoordination, ataxia and paralysis. The treated birds of group C showed no antemortem symptoms.
The main necropsy findings were small hemorrhages through out the body muscles, hepatomegaly, spleenomegaly, enlarged heart, swollen kidneys and gastroenteritis in group A and B, hepatomegaly and gastro enteritis in group C. Haematological alterations were studied in all the groups i.e. A, B, C and D. There was no significant difference found in packed cell volume, hemoglobin concentration, total erythrocytic count amongst all the groups, at both the ages. The total leukocytic count showed a significant increase in group A and B as compared to control group D. No significant increase was noted in group C as compared to group B and no significant increase between 28 and 35 days of age in groups A, B and C was noted.
The main histopathological lesions of liver and kidney included cloudy swelling, necrosis, and leukocytic infiltration in all treated groups i.e. A,B and C. Histopathological lesions o-f spleen included presence of focal areas of necrosis in group A and group B after 28 and 35 days of age. In group C after 28 and 35 days of age the spleens were normal. Histopathological lesions of heart were haemorrhage and cloudy swelling in the myocardium in group A and B after 35 days of age. No lesions were observed in all the treated groups after 28 days of age and in group C after 35 days of age. No changes were observed in liver, heart, spleen and kidneys of group .D (control).
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60.
A Study On The Pathology Of The Liver & Total Serum Bilirubin In Buffaloes Infected With Gigantocotyle Explanatum
by Akram, M | Zafar Iqbal Chaudry | Asif Rabbani | Manzoor Ahmed Basra | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1992Dissertation note: Due to neglect of proper hygienic measures, the water buffaloes are subjected to many snail-borne parasitic diseases. Amongst these diseases, paramphistomiasis due to bile duct fluke Gigantocotyle explanatum has been reported from many tropical countries including Pakistan. This parasite has been reported to cause various degrees of hepatic dysfunction in the buffaloes.
In the present study, 450 buffaloes were examined for the record of prevalence of explanatum infection. The age dependent prevalence recorded in 3 age groups i.e. upto 2 years, 2-5 years and above 5 years, was 18.2%, 30.5% and 61.4% respectively. Majority of the buffaloes in age groups upto 2 years and 2-5 years were lightly infected (100% and 78.8% respectively). Moderate infection was observed in 2-5 years and above 5 years age groups (2 1.2% and 33.3% respectively). Heavy infection (45%) was only observed in buffaloes above 5 years of age.
The pathological lesions produced by G. explanatum were observed in infected livers from groups A(upto 2 year), B(2-5 years) and C(above 5 years). Grossly, the notable changes in the size, color, consistency and appearance were recorded only in heavily infected buffaloes above 5 years of age (group C). The parasites were mostly seen attached to the common bile duct mucosa. Only in heavily infected buffaloes the parasites were seen attached to intra-hepatic bile ducts mucosa. Lentil sized nodules were observed at the site of attachment of the parasites. The walls of affected bile ducts were thickened and lumina were dilated to accomodate the parasites.
The microscopic lesions included degeneration and desquamation of epithelium of affected bile ducts. The subepithelial mucous glands showed hyperplasia. Mono-nuclear cells infiltration was observed in the subepithelial tissues. Proliferation of fibrous connective tissue was seen on the outermost layer of the affected bile ducts. Disruption of hepatic cords and dilatation of sinusoids were mostly observed in the areas adjacent to affected bile ducts. The portal tracts showed fibro-cellular reaction.
The serum samples from infected groups A, B and C and control groups A1, B1 and C1 were analysed for the study of serum total bilirubin patterns. The serum bilirubin level gradually increased with the increase in degree of parasitic infestation. The serum bilirubin level increased significantly in infected buffaloes above 2 years of age (group B and C) as compared to infected buffaloes below 2 years of age (group A). In the case of buffaloes above 5 years of age (group C), there was a significant increase in serum total bilirubin level in infected group as compared to control group. The major cause of increase in bilirubin level, in this case, was heavy parasitic infestation.
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61.
A Study On The Incidence And Pathology Of Hydropericardium Syndrome In Broilers
by S. Israr H. kazmi | Manzoor Ahmed Basra | Asif Rabbani | Zafar Iqbal Chaudry | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1993Dissertation note: Prevalence of HPS was recorded by surveying 55 broiler farms in and around Lahore between September 1990 to December 1990. Out of these 55 broiler farms 12 farms were affected with HPS. From 12 affected farms 15225 birds died due to HPS, so the average mortality recorded was 16.36 percent. Indicence of HPS recorded was maximum between 3rd to 5th to week of age, however birds of younger & older age groups were also affected with HPS. Different breeds & feeds have no affect on prevalence of HPS. HPS inoculum prepared from morbid livers, when inoculated into 10 days old, twenty embryonating eggs with 0.2 ml through chorioallantoic membrane route, caused 100% mortality within 3-6 days post inoculation.
Day old, 120 broiler chicks were reared and divided into four groups A, B, C, D each comparising of 30 chicks. At the age of 21 days, broiler chicks of A, B and C groups were inoculated against HPS through various routes i.e. oral, subcutaneously and Intramuscularly, respectively, while D group remained control. Mortality was recorded for the experimental period of 12 days. It was noted that the broiler chicks in group A, 3 birds died having 10% mortality, in group B, 17 birds died having 56.66% mortality while in group C, 14 birds died having 46.66% mortality.
Mortality was maximum within first 5 days, whereas mortality become minimum in the last 4 days of the experimental period. Common clinical signs observed were sudden increase in mortality, depression, yellowish diarrhoea, huddling of the birds.
The histopathological changes in affected broiler chicks included ballooning of the pericardial sac with blood stained (amber) fluid. The pericardial fluid varied from 0.5 to 2.5 ml. The heart become flabby, rnisshaped and enlarged. Microscopically, cardiac muscles showed degeneration, mild congestion, leukocytic infiltration. Liver appeared swollen, enlarged, friable, dark brown in colour and show mottling. Pin point subscapular haemorrhagic lesions observed in markedly fatty livers. Microscopically, liver showed fatty changes, massive necrosis, dilatation of sinoids, nuclei become larger than normal. Light basophilic intranuclear inclusion bodies of different sizes observed in liver parenchymoal cells. The kidneys become swollen, enlarged, friable and dark brown to pale yellow in colour. Microscopically, kidneys showed tubular dilatation, degeneration and necrosis. The glomeruli were misshaped and showed glomerunephritis. The thymus did not exhibit any gross or histopathological changes.
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62.
Etiology Of Hypertension In Adult Human Beings
by Tariq Ismaeel, Qazi | Manzoor Ahmed Basra | Tufail Muhammed Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1992Dissertation note: A total number of 80 hypertensive c±€ idividuals and 40 normal control individuals of 40 - 60 years age included in this study. Out of 80, 40 males of 40-60 years of age and 20 males out of 40 control of same age were placed in major group A and AC.40 females of 40-60 years of age and 20 control of same age were placed in major group B and BC. The major groups A, AC, B and BC were further subdivided into A1, A2, A3, A4 AC1, AC2, AC3 AC4, B1, B2, B3, B4, BC1, BC2, BC3 and BC4 according to the age group as followings:-
A1, AC1, B1, BC1 (40-45 years of age).
A2, AC2, B2, BC2 (46-50 years of age).
A3 AC3, B3, BC3 ( 51-55 years of age).
A4, AC4, B4, BC4 (56-60 years of age).
From all individuals blood samples were collected and Serum was separated. The Serum was transferred to clear plastic bottles labelled and stored at - 20 C untill use for the analysis of Serum Cholesterol and Serum Sodium levels. Serum cholesterol and Serum Sodium estimations were done by connercial kits.
The mean Serum Cholesterol in normal adult males were 188 ± 8.41 194.6 ± 6.42, 194 + 14.47 and 191.8 + 20.57 mg/lOO ml in sub groups AC1 AC2, AC3 and AC4 respectively. While in hypertensiveobese males the mean Serum Cholesterol were 281.4 ± 56.46, 291.1 ± 28.61, 286.1 ± 45.90 and 279.9 + 41.19 mg/100 ml in sub groups A1, A2, A3 and A4 respectively. The mean Serum Cholesterol in normal adult females were 179.6 ± 15.54, 174.8 ± 9.54, 177.6 ± 13.42 and 176.8 + 5.12 MG/100 ml in sub groups BC1, BC2 BC3 and respectively. While in hypertensive obese female the mean Serum Cholesterol were 278.4 ± 42.21, 269.8 ± 36.72, 266.6 ± 51.96 and 271.3 ± 45.99 in sub groups B1, B2, B3 and B4 respectively.
These results clearly show that there was significant difference (P < 0.05) present between mean Serum Cholesterbi of individuals in different physiological status. The mean Serum Cholesterol of different age groups of normal and hypertensive obese individuals were not significantly different (P<O.Ol). The mean values of rum Sodium levels in normal adult males were 146.8± 3.42, 145.6 ± 8.29, 147.8 ± 5.01 and 145.6 ± 4.03 mmol/L in sub groups AC1, AC2, AC3 and AC4 respectively. While in hypertensive obese males it was 167.8 ± 8.88 172.1 ± 6.45, 168.6 ± 8.56 and 169.9 ± 9.19 mmol/L in sub groups A1, A, A3 and A4 respectively. The mean Serum Sodium levels in normal adult females were 144.2 ± 7.29, 144.6 ± 7.30, 147.4 ± 4.77 and 147.6 ± 3.28 mmol/L in sub groups BC1, BC2, BC3 and BC4 respectively. While in hypertensive obese females the mean Serum Sodium levels 168.8 + 8.74, 167.4 ± 10.28, 168.6 ± 8.99 and 168.5 ± 11.06 mmol/L in sub groups B1, B2, B3 and B4 respectively.
The results of Serum Sodium levels show that there was significant difference (P <0.05) present between the mean Serum Sodium levels of individuals in different Physiological Status. The mean Serum Sodium level of different age groups of normal and hypertensive obese individuals were not significantly different (P<0.01).
Significant difference (P < 0.05) of Serum Cholesterol of individuals of different socioeconauic classes, different grades of hypertensicn and different degree of deviation fran standard weight were also noted. While in case of Serum Sodium level there was significant difference (P <0.05) in the individuals of different socioeconanic classes and there was no significant difference (P<0.05) in the individuals of different degree of hyperbension and different degree of deviation from standard weight.
The findings of present study will help in the diagnosis and management of hypertension and will prove beneficial for screening Of the cases from general population.
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63.
Pathology Of Experimentally Induced Stunting Syndrome In Broilers
by Ghulam Mustafa | Dr. Shakil Akhtar Khan | Dr. Mian | Dr. Shahzada Khurram Ashraf | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1993Dissertation note: One hundred and twenty, day-old commercial broiler chicks were obtained. These chicks were divided randomly into two equal groups i.e. control (A) and treatment (B). Each chick of both the groups was weighed and then dosed with one ml of either tryptose phosphate broth (A) or prepared inocula (B) at the same day. Chicks of both the groups were housed separately under similar standard managemental conditions. By 1-2 days of inoculation it was observed that the affected birds of group-B were having pasty vents and diarrhoea of yellow to brownish color. They were having a hunched appearance, ruffled feathers and drooping wings. By 4- 5th day of age an unevenness in the size of inoculated birds was quite obvious. By second week of age some underweight birds had feather developing problems and they were lame showing the lesions of tenosynovitis. In the subsequent period of experiment it was noticed that these severely affected birds had few ruffled, short broken-shaft mature wing feathers and in the later stages of experiment these birds had pale combs, wattles and shanks. During 28-days experiment 20% of the inoculated birds died while none of the bird died amongst the control group.
Each chick of both the groups was weighed at the end of every seven days upto day-28 of the experiment. While to determine the "Feed Conversion Ratio" (FCR) sufficient amount of feed was provided to each group and the remaining feed in feeding troughs was calculated at the same weekly intervals. It was statistically concluded that birds of group-B remained significantly lighter in the weight at all the stages of experiment with severely impaired "Feed Conversion Ratio".
By 29th day of the experiment 23-stunted birds from group-B and an equal number of normal birds from group-A were slaughtered, all of these birds were weighed prior to and post slaughtering. Then the weight of skin, dressed carcasses, muscles, bones, and all the internal organs was also ascertained and their ercentage weight relative to the respective live weight of bird was also determined. During the dressing the gross lesions observed were also recorded. It was observed that the stunted birds were severely emaciated and their skeleton was brittle which broke easily during dislocation. The intestine of the affected birds were palor and filled with partially digested feed and gases. The pancreas and the lymphoid organs were severely atrophied. The lungs, kidneys and the liver were congested, while the heart was having the lesions of focal rnyocarditis.
Then some of the affected organs (intestines, pancreas, liver, spleen, kidney, femur bone and muscles) were processed for histopathological examination. Although all of these organs were having the characteristic changes hut amongst them the pancreas was the most severely affected organ which revealed the classical histopathological alterations necessary for understanding the pathogenesis of the syndrome. Whereas affected spleen presented reduced number of lymphoid follicles with less number of lymphocytes per follicle indicating incidence of immuno-suppression. The datas obtained during present study were analysed statistically by applying student's t-test.
Hence we can estimate a successful induction of the syndrome by a crude intestinal homogenate prepared from the affected birds under local conditions.
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64.
Study Of The Pathogenesis Of Oedema In Broiler Chicks Affected With Hydropericardium Pulmonary
by Shirin Munir | Dr. Shakil Akhtar Khan | Dr. Rashid | Dr. Shahzada Khurram Ashraf | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1993Dissertation note: For this research project 120 one-day old broiler chicks were divided into two groups i.e., A and B. The birds in group B were challenged with the H.P.S. agent at 15 days of age, whereas those in group A were dealt with as control birds. After the appearance of the syndrome serum samples were obtained from the diseased as well as control birds and then they were all perfused with 1% silver nitrate solution. Tissue samples from heart, liver, lungs, kidneys, spleen and bursa of fabricius were collected, processed and slides were prepared and stained by Haematoxylin and Eosin staining technique. In addition, tissue sections from all the organs were also stained by Silver nitrate impregnation technique.
The most peculiar post-mortem finding was over distension of the pericardial sac with clear or amber coloured fluid. The heart was mis-shaped, flabby with petechial haemorrhages on the epicardium. The liver was congested, swollen, friable with multiple pale areas of necrosis. Kidneys were enlarged, congested and dark in colour. The renal tissue was found to be much friable. Lungs were oedematous, congested and dark. The spleen showed congestion and haemorrhages. Bursa of fabricius was found to have normal appearance in some cases while in others showed slight atrophy.
For this research project 120 one-day old broiler chicks were divided into two groups i.e., A and B. The birds in group B were challenged with the H.P.S. agent at 15 days of age, whereas those in group A were dealt with as control birds. After the appearance of the syndrome serum samples were obtained from the diseased as well as control birds and then they were all perfused with 1% silver nitrate solution. Tissue samples from heart, liver, lungs, kidneys, spleen and bursa of fabricius were collected, processed and slides were prepared and stained by Haematoxylin and Eosin staining technique. In addition, tissue sections from all the organs were also stained by Silver nitrate impregnation technique.
The most peculiar post-mortem finding was over distension of the pericardial sac with clear or amber coloured fluid. The heart was mis-shaped, flabby with petechial haemorrhages on the epicardium. The liver was congested, swollen, friable with multiple pale areas of necrosis. Kidneys were enlarged, congested and dark in colour. The renal tissue was found to be much friable. Lungs were oedematous, congested and dark. The spleen showed congestion and haemorrhages. Bursa of fabricius was found to have normal appearance in some cases while in others showed slight atrophy.
The histopathological study of tissue sections stained by H&E staining technique revealed necrosis, accumulation of mononuclear cells and haemorrhages in the heart. The characteristic feature of liver was coagulative necrosis with disruption of hepatic cords. Some hepatocytes had basophilic intranuclear inclusion bodies. Renal tissue showed massive necrosis and degeneration of tubular epithelium along with lymphocytic infiltration. The lungs had oederna and mononuclear cell infiltration. The spleen showed degeneration, necrosis and haernorrhages while bursa of fabricius exhibited disruptions in the epithelial covering of its follicles.
Silver nitrate impregnation explored distinct changes in the blood vessels. There was disruption of the capillary endothelium along with accumulation of oedema fluid around the capillaries and an increase in the size of intercellular spaces.
The data, obtained by the analysis of serum, statistically indicated that the concentration of serum sodium, potassium, urea and uric acid increased greatly whereas the total serum proteins were markedly decreased in the affected birds. Thus it can be concluded from this study that disruption of the capillary endothelium and severe damage and malfunctioning of the heart, liver and kidneys appear to play a predominant role in the development of edema in this syndrome.
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65.
Effects Of Copper Sulphate Medication On Different Organs & Serum Copper Level In Broiler Chicks
by Azhar Saqib | Dr. Manzoor Ahmed Basra | Dr. Mubashar | Dr. Shakeel Akhtar Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1993Dissertation note: The present work was planned to study the effects of copper sulphate medication in feed in broiler chicken. For this experiment 90 broiler chicks were reared under standard managemental conditions for 30 days. All the birds weze weighed and their weights were recorded. Then these were divided into three groups, i.e., Groups A, B and C, each group comprising of thirty chicks.
Group A was given 500 mg and Group B was given 1000 mg copper sulphate per kg of feed, for one week. At the end of the experimental period the birds were again weighed, blood of the birds was collected, the birds were slaughtered and the tissues were taken. The blood was subjected to serum-copper analysis and the tissues of Liver, Kidney, gizzard and intestine to histopathological changes.
Clinically the birds showed anorexia. There was increased water intake, severe depression and paralysis in mild case of poisoning. In severe case in addition to the above conditions there was loss of weight, blood stained diarrhoea, ruffled feathers and, the birds lethargy and anaemic. The most important, gross postmortem finding observed was fluid filled abdomen, and peritoneal hemorrhages. There were areas of etosion and necrosis throughout the length of the gastro-intestinai tract. The liver was pale in appearence, with a slight increase in the weight and fragile consistency. The kidneys exhibited severe inflammatory reaction in the form of congestion and swelling.
Histologically the liver showed vascular congestion, vacuolization due to fatty degeneration and leucocytic infiltration. The sinusoidal channels were reduced. In severe cases there was proliferation of fibroblasts. The hepatocytes showed margination of the chromatin material. In kidneys the renal epithélium was the most effected portion by necrosis and karyorrhexis. There was vascular congestion and leukocytic infiltration. The intestines and the gizzard both showed wide spread areas of necrosis and erosion with thickened lining showing fibrotic areas and the cells of the lining mucosa and sub-mucosa desquamated. The muscular layer of the gizzard showed dystrophy.
The weights of the birds were recorded at the age of 37 days before slaughtering. The results were analysed statistically. There was a significant decrease in the weights in groups A and B as compared to control group C. The serum- copper level was also estimated and analysed statistically. There was a significant increase in serum copper contents in Groups A and B, as compared with the Control Group C.
Little work has been reported on the effects of copper sulphate mediation on the different organs and serum-copper level, in Pakistan. The results of the present study has helped us to assess the effects of copper sulphate medication on different organs, serum-copper level and weight gain in broiler chicks.
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66.
The Incidence And Pathology Of Neoplasms Of Buffaloes In Sargodha District
by Khalid Bashir | Dr. Shakeel Akhtar Khan | Dr. Muhammad | Dr. Shahzada Khurram Ashraf | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1993Dissertation note: One hundred and forty tissue specimens (suspected neoplasms) were obtained from buffaloes in District Sargodha. These specimens were examined macro and microscopically. It was found that 37 neoplasms were benign and 63 neoplasms were malignant. The benign connective tissue tumours accounted for fibroma (12), melanoma (5). Benign epithelial tumour included papillorna (20). The malignant connective tissue tumour was sarcoma lympho(20) and the malignant epithelial tumour included ocular squamous cell carcinoma (35), horn cancer (6) and Adeno-carcinoma (2).
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67.
The Incidence And Pathology Of Neoplasms Of Cattle In District Mirpur Azad Kashmir
by Ahmad Rafi | Dr. Shakeel Akhtar Khan | Dr shahzada khurram Ashraf | Dr. Mohammad | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1993Dissertation note: A survey was conducted in Bhimber, Sammani and Iftikharabad areas of District Mirpur, Azad Kashmir to record the incidence of neoplasms among the cattle. One hundred and thirty tissue specimens (suspected neoplasms) were obtained either by biopsy or from slaughter houses. The tissue specimens were examined macroscopically and microscopically.
Out of total tissue specimens, 100 samples (43 biopsy samples and 57 morbid samples) proved neoplasms. Neoplasms accounted for 74 malignant and 26 benign. These neoplasms included 34% Bovine Ocular Squamous Cell Carcinoma, 16% I-Torn Cancer, 6% Carcinoma, 2% Adenocarcinoma, 16% Lymphosarcoma, 14% Papilloma, 6% Melanoma and 6% Fibroma.
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68.
A Study On The Pathology Of Internal Genital Organs Of Female Canmel (Camelus Dromedarius)
by Yasser Mustafa Butt, M | Dr. Shakil Akhtar Khan | Dr. M. Athar | Dr. Shahzada Khurram Ashraf | Faculty of Veterinary Sciences.
Material type: ; Format:
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; Nature of contents: ; Literary form: Publisher: 2009Dissertation note: A total of 260 ovaries, 260 oviduct, and 130 cervices of 130 female camels including 15 female camel calves, 34 female camel heifer and 81 adult female camels were collected from the Lahore abattoir.
Biometrical values of normal ovaries, oviduct and cervices also determined. The biometrical values of both left and right ovaries comprising of length, width, thickness and weight were recorded. Statistically there was no difference in the measurements of both left and right ovaries which however, increased with the advancement of age of camel.
The mean length of the oviducts of these 3 age groups of female camels determined. There was no difference between the mean length of both left and right oviduct of same group but difference between the group and the length of the oviducts increased with the advancement of age.
The mean length of cervices and cervical annular rings of female camels of the 3 age groups were also determined. The mean length of cervices correlated well with the number of rings.
The incidence of diseased condition of ovaries and cervices in the 3 age groups were also noted which revealed that the incidence of diseased condition of ovaries in camel calves was nil and in heifer ovarian hypoplasia (2.94%) on left ovaries and follicular cyst (1.23%) in adult female camels on the left ovary.
The incidence of diseased condition of cervices in camel calves was nil, mucocervix (2.96%) in heifers and 2.46% including mucocervix (1.23%) and cervicitis (1.23%) in adult camels. The multiple diseased condition was found (1.53%) out of 130 genitalia.
The gross and histological findings of each diseased conditions were studied.
Haematological parameters i.e. Total leukocytic count and Differential leukocytic count studied in both normal and diseased conditions. The normal Total leukocytic values were higher in calf than heifer and adult. Differential leukocytic values were higher in adult. The neutrophils numbers were higher while basophils were lower. The values of Total leukocytic count and Differential leukocytic count were also increased in diseased condition.
The biometrical norms of ovary, oviduct and cervix have been established which could be used as yard stick in diagnosis of pregnancy in female camels. The study of diseased conditions of. ovary and cervices has line-lighted the type of disease and their extent in camel. The haemotological studies including Total leukocytic and Differential leukocytic count in both normal and diseased conditions will also helpful. Based on the information, prophylactic and control measure can better be formulated.
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69.
A Study On Pathology And Haematology Of Broiler Chickens Experimentally Infected With Salmonella
by Younus, M | Dr. Shahzada Khurram Ashraf | Dr. Mubasher | Dr. Shakeel Akhter Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1994Dissertation note: The investigation was conducted to study the pathology and Haemotology of broiler chickens suffering from experimental salmonella gallinarum infection. Total of 140 day did broiler chicks were reared upto 3 weeks of age and were fed on commercial poultry feed. The birds were randomly divided into four groups i,e. A, B, C and D containing 35 chicks in each group. The inoculation of broiler chicks of experimental groups were done at the age of two weeks at a dose rate of 1ml per bird through different routas ic group A through oral route, group B through subcut, group C through I/rn and group D was kept as control. All the groups were kept under close observation to record signs and symptoms. At the age of 3 weeks (7 days of post infection) the birds of experimental and control groups were slaughtered for the study of haematological parameters (Fib estimation, TEC and TLC), gross and histopathological lesions in visceral organs (Heart, Liver, lung, spleen and kidneys)
Antemortem symptoms were recorded throughout the experiment in all the infected groups and observed listlessness with ruffled feathers, droopy wings, poor growth, weakness, loss of appetite, adherence of whitish material to vent, laboured breathing, slight depression and increased thirst. Conjunctivitis and arthritis in few cases were observed.
The postmortem changes observed in all the infected groups A, B and C at the age of 21 days (7 clays post infection) included enlarged heart, necrosis of myocardium, pericarcil tis, enlarged creamy white liver with pin point haemorrhages, congested lungs with necrotic loci ple.nomegaiy. and swollen kidneys with c1isco1.oiration and triable consistency. No lesions could be observed in control group D.
Haemotological alterations were studied in all the groups i ,e A, B, C and D. There was a significant decrease in haemoglobin concentration, total erythrocytic count amongst all the infected groups A, B and C at 21 days of age (7 clays post infection) as compared to control group D. but: the total leukocytic count showed a significant increase in all the infected groups, i, e. A, B and C as compared to control group D at the 21 clays of age.
The main histopathological lesions observed in al .1 the infected groups A, B and C at the age of 21 clays (7 clays of post infection) in heart, liver, lungs, spleen and kidneys included necrosis, haemorrhage and leukocytic: infiltration. However, in few cases fatty destrophy, congestion and degenerative changes were also observed. No histopathological lesion could be observed in control group D.
From present study it was concluded that fowl typhoid caused by Salmonella gallinarum infection resulted in higher mortality and pathological changes especially through intramuscular route as compared to other routes of infection.
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70.
Effect Of Vitamin E Deficiency And Excess On Immune System Of Broiler Chickens
by Fauzia Kaukab Bashir | Dr. Shakil Akhtar Khan | Dr. Muhammad | Dr. Shahzada Khurram Ashraf | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1994Dissertation note: This study was carried out to investigate whether different dietary levels of vitamin E (recommended, deficient and excess) had any detrimental or beneificial effect on body weight gain, lymphoid organs, haemalotogical values, humoral response to NDV-vacci nation, eel I ular response to tuberculin and phagocytic activity of neutrophils of broiler chickens.
The study indicated that vitamin E supplementation 300 lU/Kg feed had beneficial effects on the weight gain of birds, weight of lymphoid organs such as bursa of fabricius, spleen, thymus and liver; feed intake and feed conversion ratio; serum antibody development against NDV vaccination; phagocytic index and delayed hypersensitivity index. Whereas, vitamin E deficiency adversely affects the body weight gain; weights of lymphoid organs such as bursa of fabricius, spleen, thymus and liver; feed intake and feed conversion ratio, serum antibody development NDV vaccination; Phagocytic index and delayed hypersensitivity index.
No significant effect of vitamin E supplementation/deficiecy was noted on haematological values.
From the results of this study it is concluded that vitamin E is not an immunosuppressive agent when used at recommended leavels or even in excess. Vitamin E deficient level in diet lowersthe immunocompetance of broiler chicken, whereas vitamin E excess level in diet improved immune responses and body weight gain.
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71.
Studies On Blood Chemistry And Histopathological Alteration Induced By Mycoplasma Gallisepticum In Broiler
by Shahida Parveen | Dr. Shakil Akhtar Khan | Dr. Asif | Dr. Shahzada Khurram Ashraf | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1994Dissertation note: For this project 120 one-day-old broiler chicks were divided into two equal groups i.e. A and B. The birds in group B were challenged with Mycoplasma gallisepticum at 7 days of age, whereas those in group A were dealt with as control birds. After 21 days of post infection serum samples were obtained from the diseased as well as control birds during slaughtering. Tissue samples from lungs, liver and trachea were collected and processed for histopathological examination.
The postmortem findings revealed catarrhal exudate in the flares, sinuses, trachea, lungs and oedema of airsacs wall. The lungs were congested, dark in colour and inflammed. Liver showed fibri nopurulent perihepatitis, haemorrhages and congestion. Trachea was found to have a tenacious viscous cloudy exudate which was adherent to the tracheal wall.
The histopathological sections showed oedema, haemorrhages, congestion, massive infiltration of monocytes and heterophils in the lungs. The liver showed evidence of necrosis, haemorrhages and infiltration of heterophils, lymphocytes in interlobular spaces around portal vessels. The trachea showed epithelial and submucosal infiltration with lymphocytes, hypertrophy of epithelial mucous glands' and some catarrhal exudate on the mucosal surface. Sloughing of mucosa with variable degree was noted.
The data, obtained by the analysis of serum, statistically indicated that the concentration of serum uric acid, serum glutamic pyruvic transaminase, serum alkaline phosphatase and serum lactic dehydrogenase was increased significantly in infected birds.
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72.
Comparative Study On Pathology Of Hemic System Of Three Broiler Chicken Strains Suffering From Experimental
by Farrukh Hasan Zaidi | Dr. Shakil Akhtar Khan | Dr Mubashar | Dr. Shahzada Khurram Ashrrf | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1994Dissertation note: Three hundred day old broiler chicks of three different strains (one hundred of each strain) were obtained from the market. The strains were Hubbard (H) Lohmann (L) and Indian River (IR). The chicks of each strain was divided into two groups of equal number, which were further subdivided into two subgroups. The subgroups Al, A2 ; C1, C2 ; and El, E2 were kept as uninoculated control and subgroups B1, B2 ; Dl, D2 and Fl, F2 where inoculated with HPS antigen subcutanoously at the age of 21 days. The number of diseased chicks who died or slaughtered were counted. The maximum number of chicks showed HPS and died and slaughtered, i.e 66% belonged to H, followed by 30% which were JR and then the L which were 24%.
The haematologlcal values of all the broilers affected with lIPS were determined. In Hubbard strain the mean ±S.E of TEC was 2.02 ± 0.06 million percubic millimeters, TLC 12.57 ± 0.51 thousand per cubic millimeter, Thrombocyte count 28.60 ± 0.40 thousand per cubic millimeter, Hb content 6.51 ± 0.16, PCV 26.05 ± 0.58, ESR 3.63 ± 0.21. The thousand per cubic milimeter. The PLC were also determined which included lymphocytes 55.16 ± 0.54, monocytes 3.00 ± 0.32% heterophils 40.26 ± 0.39%, eosinphils 0.89 ±. 0.18% and basophils 0.68 ± 0.15.
In Lohznann strain the mean j S.Eof TEC was 2.14 ± 0.11 million per cubic millimeter, TLC 18.85 ± 0.2.4 thousand per cubic millimeter, Thrombocyte count 28.42 ± 0.53 thousand per cubic millimeter, Hb content 7.51 ± 0.21, PVC 27.14 ± 0.68, ESR 3.28 ± 0.28. The DLC were also determined which included lymphocytes 58.28 ± 0.82%, moaocytes 3.80 ± 0.31%, heterophils 36.00 ± 0.90%, eosinophils 1.14 ± 0.24% and basophils 0.7l ± 0.26.
In Indian River strain the mean ± S.E of TEC was 2.08 ± 0.07 million per cubic millimeter, TLC 16.10 ± 0.78 thousand per cubic millimeter, thrombocyte count 28.58 ± 0.53, Hb content 7.38 ± 0.15, PVC 26.8 t. 0.58, ESR 3.20 ± 0.24. The DLC were also determined which included lymphocytes 55.60 0.47%, monocytes 3.30 . 0.28, heterophils 39.30 ± 0.49%, eosinophils 0.90 .0.17 amd basophils 0.80 ±. 0.27.
Gross pathological lesions in the inoculated bizds were studied. The heart showed ballooning due to distention of pericardial sac with pericardial fluid. Other lesions Included haemorrhages on the epicardium and flabbiness of the mycordium .tJnder the microscope, degenerative changes in myocordial tissue were seen. Lesions in the spleen included splenomegaly and haemorraghic spots. Histopathologically there were degeneration, necrosis, and haemorrhages. Aorta showed flabbiness of the wall. The disruption of endothelium was also discerned.
Keeping in view all the above findings, It Is concluded that there is a marked difference in uaceptibility of HPS among three different commercial broiler strains and HPS also effects a lot on the hemic system of the broiler. The Hubbard broiler strain is more susceptible which is followed by the Indian River and Lohmann respectively.
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73.
Effect Of Vitamin A Deficiency And Excess On Immune System Of Broiler Chickens
by Ahmad Raza | Dr. Shakil Akhtar Khan | Dr. Haji Ahmad | Dr. Shahzada Khurram Ashraf | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1994Dissertation note: This study was conducted to investigate the effect of different dietary levels of vitamin A (Excess, deficient and recommended) on body weight gain, lymphoid organs, haematological values, humoral response to NDV-vaccination, cellular response to tuberculin and phagocytic activity of neutrophils of broiler chickens.
The results of the study revealed that vitamin A supplementation at 20,000 1U/Kg feed had beneficial effects on the weight gains of birds; weight of lymphoid organs such as bursa of Fabricius, spleen, thymus and liver; serum antibody development against NDV vaccination; feed intake and feed conversion ratio; phagocytic index and delayed hypersensitivity index. Whereas, vitamin A deficiency had adverse effects on body weight gains; weight of lymphoid organs, serum antibody development agaist NDV vaccination, feed intake and feed conversion ratio, phagocytic index and hypersensitivity index.
No significant effect of vitamin A supplementationldeficiency was noted on haematological values.
From the results of this study it can be concluded that vitamin A is not an immunosuppressive agent when used at recommended level or even in excess (for short duration). Vitamin A deficient level in diet had detrimental effects on immune system in terms of lowered humoral and cellular responses and also decreaed phagocytic activity of neutrophils. Whereas, vitamin A supplementation at excess level improves cellular and humoral responses along with improved phagocy bic activity of neutrophils.
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74.
Study On The Pathlogy Of Leg Deformity In Broiler Chickens
by Farrukh Khurshid | Dr. Shakil Akhtar Khan | Dr. Haji Ahmad Hashmi | Dr. Shirin Munir | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1994Dissertation note: The project was designed to. study the pathology of legdeformity in broiler chickens. Fifty leg deformed broiler chickens were collected from 30 commercial poultry farms of Lahore, Kasur and Pattoki. Abnormal posture or gait, reluctanceto walk and twisted, bent, bowed or rotated legs were the presenting signs. Routine post-mortem examination was performed to observe qualitative (severity and direction of deformity, position of gastrocnemius tendon, subjective assessment of bone strength, swollen and enlarged joints) assessment of bones. Four cases of varus, 30 cases of valgus, 14 cases of slipped tendon, 2 cases of dislocated condyles were recorded, in gross pathology 30 cases of dyschondroplasia, 4 cases of rickets, 7 cases of thickening of tendons, 1:3 cases of synovitis, 5 cases of femoral head necrosis, 1 case of bone abcess and 8 cases of curly toes were observed. It was observed that male birds had greater incidence of leg deformity than females Hubbard strain had greater incidence than other strain and It was noted that the most important age of deformity was 2nd and 3rd week of age and Hubbard birds grew fastly at this age and more prone to leg deformity. The affected portions of bones and tendons were processed for histopathological examination.
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75.
Pathology Of Infectious Bursal Disease Following Challenge In Broiler Chicks Vaccinated With Three Different Vaccines
by Mamoon Rashid Chaudhry | Dr. Shakil Akthar Khan | Dr. Ahmad Raza | Dr. S. Ata-ur-Rehman | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1996Dissertation note: This project was designed to study the pathology of IBD following challenge in broiler chicks vaccinated with three different commercial imported live virus vaccines i.e. [BUR-706 (V1), D-78 (V2) and Bursine-2 (V3)]. A total number of 240 day old broiler chicks were randomly divided into eight groups, A through H, each containing 30 birds. Groups A, B and C were vaccinated with vaccines V1, V2 and V3 respectively as per schedule of the manufacturers and were challenged at 30 days of age with the local IBD strain procured from Veterinary Research Institute (VRI), Lahore intraocularly, while groups D, E, and F vaccinated with the V1, V2 and V3 were kept unchallenged. The group G nonvaccinated but challenged at 30 days of age was kept as control. The group H, which was non vaccinated and nonchallenged also kept as control.
All the birds were challenged at day 37 of experiment, lymphoid organs (bursa of Fabricius, spleen and thymus) to body weight indices were determined. These organs were also gross and histopathologically examined. Serum samples of each bird was analyzed for estimation of antibody titre by applying Agar Gel Precipitation technique. The experimental data was statistically analysed by complete randomized design.
The clinical signs like haemorrhages in thigh and pectoral muscles alongwith mucus in intestine were noted only in the group which was non-vaccinated but challenged. Similarly the bursa of Fabricius grossly appeared severely hemorrhagic and atrophied in the birds of group G. But the bursas of the birds inoculated with the intermediate vaccines V1 and V2 were also slightly atrophied. The spleen and thymus of the birds inoculated with the field isolate but non vaccinated were slightly swollen with grey foci on their surfaces.
Histopathological study of the bursa revealed an abundance of plasma cells, macrophages and necrotic areas in the medullary areas of follicles in the group G.
The spleen showed multifocal areas of liquefactive necrosis with lymphocytic depletion in the same group which was kept non vaccinated but challenged. The thymus showed no any marked histologic lesion.
All the inoculated birds were found positive for the Infectious bursal disease virus antibodies.
The statistical analysis of the lymphoid organ: body weight index of bursa showed a highly significant difference in the birds challenged but non vaccinated and the birds either vaccinated challenged or vaccinated nonchallenged and non inoculated birds. The lymphoid organ: body weight indices of spleen and thymus showed non significant difference amongst all the birds. Hence from this study it can be concluded that all three imported vaccines were some what equally efficacious in preventing clinical IBDV infection, except the little immunosuppressive effect of the two intermediate strain vaccines.
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76.
Pathological Changes Associated With Experimental Infectious Bursal Disease In Broiler Chickens
by Nyla Dil Tarar | Dr. Shakeel Akhter Khan | Dr ..S. Ata-Ur- Rehman Rizvi | Dr. Ahmed Raza | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1995Dissertation note: This project was designed to study the pathological changes associated with experimental Infectious bursal disease in broiler chickens. Two hundred and forty broiler chicks on 21 days of age were randomnly divided into six groups, A through F, each containing 40 birds. The inoculum of Infectious bursal disease virus was inoculated intraocularly in the chicks of group A at the age of 21 days, in group C at the age of 28 days, and in group E at the age of 35 days. Groups B, D and F were kept as control for groups A, C and E respectively. Blood samples and lymphoid organs of seven randomnly selected chickens from each group were collected at day 4 post inoculation (P1), 8 P1 and 12 P1, and haematology (TLC, DLC), serology (AGPT) and histopathology of lymphoid organs were conducted.
Typical clinical signs of IBD were noted. The chief and consistent gross lesion produced in the chickens infected at different ages was an initial increase in the size of bursa. At day 4 P1 bursa was oedematous and double in size but at days 8, 12, P1 it was smaller in size than in control chickens. The spleen and thymus appeared almost normal grossly except a few gray foci on spleen.
Histopathological study of bursa of Fabricius (regardless of the age at infection) revealed an initial macrophage and heterophil infiltration of the lymphoid follicle and inter-follicular spaces followed by increased inter-follicular connective tissue, plasma cells, macrophage and thickening of bursal epithelium. Spleen showed lymphocytic necrosis and depletion in the initial stages followed by repopulation. Statistical analysis of lymphoid organ: body weight indices of spleen, bursa and thymus showed a significant difference in the inoculated and control chickens but there was no difference in the chickens inoculated at different ages. All the inoculated birds were found positive for the Infectious bursal disease virus antibodies. The results of hematological study indicated that there was a decrease in the total number of leukocyte and percentage of hetrophils and lymphocytes in all the inoculated chickens regardless of the age at the time of infection. Thus it can be concluded from this study that age susceptibility, from 21 to 35 days, among broiler chickens for Infectious bursal disease was nearly the same.
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77.
Comparative Study On The Effect Of Experimentally Produced Hydropericardium Syndrome On Some Visceral Organs of Three Different Broiler Chicken Strains
by Imtiaz Rashid Qureshi | Dr. Shakil Akhtar Khan | Dr. Haji Ahmad | Dr. Shahzada Khuram Ashara | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1994Dissertation note: Susceptibility, liver function , kidney function and gross and histopathological changes in different organs among different strains of broilers were studied in this experiment.
For this purpose 300 days old broiler chicks of three different strains (Hubbard,Lohmann and Indian River)were obtained from two different commecial hatcheries .Each strain was divided into two equal groups comprising of 50 birds each. These groups were further subdivided into two equal subqroups comprising of 25 birds each .Sub groups so formed were A1,A2,r3l,E2.Cl.C2,Dl,D2,El,E2,Fl and F2. Sub groups (Bl,B2,Dl,D2,Fl and F2) were challenged with HPS inoculum thiough S/C route on 21st day whereas groups (Al,A2,C1,C2,E1 and E2) were kept as control and inoculated with normal saline through S/C route on the same day. Clinical symptoms observed were depression, ruffled feathers, loose dropings, inappetance, disinclination to move and lowering down the head with beak point rested on the litter. Gross lesions observed were hydropericardium,hepatomagaly,nephritis, congestion and oedema of lungs. Microscopically necrosis, congestion, oedema, haemorrhages and leukocytic infiltration was recorded. For the estimation of liver and kidney function, tests for the estimation of total protein concentration and urea concentration in serum were carriedout which showed significant decrease (P<0.0l)in the total serum protein concentration and significant increase (P>0.0l) in urea concentration in the serum of experimentally challanged chicks.
This study revealed that highest mortality/slaughter occurred in Hubbard strain of broiler in which the increase in the level of urea concentration in the serum of inoculated group was the highest. Similarly lowest mortality/slaughter was recorded in Lohrnann strain in which the increase in the level of urea concentration in the serum of experimentally challenged chicks was the lowest. This study indicated that succeptibility directly depends upon the damage occurred to liver and kidneys.
The decrease in the level of total protein concentration in the serum was almost equal in all strains. As regards mortality this study exhibited that maximum mortality occurred in hubbard strain of broiler and lowest in lohmann strain which indicated that susceptibility for HPS varied among different strain of broilers.
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78.
Comparative Study On The Incidence And Pathology Of Morbid Conditions Of Uterus And Cervix Of Teddy And Local Non Descriptive Goats
by Yousaf Hassan Khera | Dr. Shakil Akhtar Khan | Dr. Ahmad Raza | Prof. Dr. Rashid Ahmed Ch | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1996Dissertation note: The project was designed to study incidence and pathology of uterine and cervical maladies in two breeds (Teddy and Local non- descriptive) of goats. Three hundred uteri and cervices (150 from each breed) of goats of three age groups, 1-2 years, 2-3 years and above three years (50 in each group) were collected from Lahore slaughter house and then thoroughly examined for any gross pathological change. The organs exhibiting macropathological lesions were subjected to morphometric, histopathological and histochemical examinations.
Of the 300 uteri and cervices, 35 (11.66%) showed morbid conditions. In 150 Teddy goats, pathological conditions were observed in, 1-2 years age group 4 (8%), 2-3 years age group 7 (14%) and in above 3 years age group 8 (16%). In 150 local non-descriptive goats, gross pathological conditions were present in, 1-2 years age group 3 (6%), 2-3 years age group 6 (12%) and in above 3 years age group 7 (14%). These results showed that incidence of pathological conditions increased with the age of the goats in both the breeds and incidence was slightly higher in Teddy goats than Local non descriptive goats in the same age groups. The following eight pathological conditions namely metritis, pyometra, pigmentation, endometrial cysts, adhesions, fibrosis, hemorrhages and aplasia of uterus were found. The morphometric, gross and histopathological changes of each disease condition of uterine horns, uterine body and cervices were studied. The histochemical evidence of tissue exhibited PAS and alcian blue reactions revealing presence of carbohydrates were also studied.
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79.
Study Of Pathogenesis Caused By Vitamin "E" Deficiency And Excess (Histopathology Of Brain, Livers, Spleen, Myocardium And Skeletal Muscles) In Broiler Chickens
by Robina Nasim | Dr. Shakil Akhtar Khan | Dr. Muhammad | Dr. Shahzada Khurram Ashraf | Faculty of Veterinary Sciences.
Material type: ; Format:
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; Nature of contents: ; Literary form: Publisher: 1994Dissertation note: The investigation was conducted to study the effects of deficiency and excess of vitamin E on growth, hematology and liver, spleen, myocardium, brain and skeletal muscles of the broiler chicken. One hundred and eighty day old broiler chicks were divided. into three groups A, B and C containing 60 chicks in each group. Group A was maintained on a feed deficient in vitamin E. Group B was maintained on a feed containing excess of vitamin E at the rate of 300 il/kg. Group C was kept as control. All the said groups were maintained on said feed for 28 days. The weights of the birds from each group were taken every week. All the groups were kept under close observations to record signs and symptoms upto the end of 4 weeks of age. 20 birds from each group were slaughtered for hematology (PCV, Hb concentration, TEC and TLC) and histopathological studies (liver, spleen, myocardium, brain and skeletal muscles). The weight of the organs (liver, spleen, heart and brain) were taken and recorded after slaughtering. There was a significant decrease in weight in group A and a significant increase in weight in group B as compared to control group C.
Antemortem symptoms were recorded throughout the experiment the birds of group. A showed loss of condition, leg weakness and slight paralysis of legs and birds of group B showed hock deformities.
The main necropsy findings were small hemorrhages throughout the body muscles more prominent on breast and thigh muscles and small pinpoint haemorrhages in brain and anemia throughout the muscles in group A. Haematological alterations were studied in all the groups i.e. A, B and C. There was no significant difference in total erythrocytic count among all the groujs. There was significant difference in packed cell volume haemoglobin concentrations and total leukocytic count. The total leukOcytic count showed significant increase in group A as compared to control group C. The main histopathological lesions of liver and spleen were necrosis and leukocytic infiltration in group A. No change was noted ub kuver abd sokeeb if group B. The brain showed necrosis, calcium deposits and haemorrhages and in myocardium there were necrosis, leukocytic infiltration and haemorrhages while in skeletal muscels necrosis, leukocytic infiltration and hyaline degeneration after 28 days of age in group A No. lesions were observed in group B and C (control)
Availability: Items available for loan: UVAS Library [Call number: 0446,T] (1).
80.
The Pathology Of Experimental Vitamin A Exess And Deficiency In Broiler Chickens
by Sami Itrat, M | Dr. Shakil Akhtar Khan | Dr. Ahmad Raza | Dr. Muhammad Athar Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
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; Literary form:
not fiction
Publisher: 1995Dissertation note: This study was conducted on broiler chickens for 7 weeks to investigate the effect of three different dietary levels of vitamin A on different body organs (oesophagus, liver, kidneys and ureters) by studying their weight and pathological lesions. The haematological parameters (TEC, Hb estimation and PCV) were also observed.
The results of the study revealed that vitamin A supplementation at 20,000 lU/Kg feed had beneficial effects on weight of organs such as oesophagus, liver, kidneys and ureters. Whereas vitamin A deficiency had adverse effects on body organs in terms of weight and morphology. No effect of different levels of vitamin A on haematological values.
Availability: Items available for loan: UVAS Library [Call number: 0447,T] (1).
81.
Studies On The Pathological Aspects Of Early Chick Mortality Due To Bacterial Infections
by Raza ur Rehman | Dr. Shakil Akhtar Khan | Dr. Shahzada Khurram Ashraf | Faculty of Veterinary Sciences.
Material type: ; Format:
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; Literary form:
not fiction
Publisher: 1993Dissertation note: The present study was conducted to investigate the bacterial etiology in the early chick mortality in Punjab. For the purpose 500 dead chicks (1-2 weeks old) were collected and subjected to the bacteriological examinations.
Various media were used for the isolation, purification and identification of bacteria including MacConkey's broth, Selenite broth, S.S. agar, E.M.B. agar MacConkey's agar, Nutrient broth, Triple sugar iron agar, Blood agar, Mannitol salt.
The isolation of bacteria was done from heart, liver, spleen, yolk sac, intestine.
The standard techniques were used for biochemical and sugar fermentation reactions. The dead chicks were also examined for gross histopathological lesions.
Of 500 birds examined 56.8% birds yielded . cjj isolates followed by . pullorum (25.4%) and S. aureus (17.8%).
Sixty chicks suffering from Escherichia, Salmonella and Staphylococcus spp. infections (Twenty chicks positive for each genera) were selected for histopathological examination. The visceral organs (liver, lungs, heart, kidneys and spleen) showing characteristic lesions of diseases from 60 chicks were collected for histopathological study.
From the foregoing, it is evident that . cli, S. pullorum and S. aureus are major bacteria involved in early chick mortality. Therefore to avoid these infections, implementation of hygienic standards both at hatchery and brooder house, strict vaccination schedule; practice of disinfections, proper husbandry practices, testing of parent flocks for carriers, and finally strict quarantine measures are suggested.
Availability: Items available for loan: UVAS Library [Call number: 0448,T] (1).
82.
Effect Of Fermacto On The Function And Morphology Of Liver And Kidneys Of Broiler Chicks
by Tahir Aziz | Dr. Shakil Akhtar Khan | Dr. Ahmad Raza | Dr. Haji Ahmad Hashmi | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1997Dissertation note: This project was designed to study the effect of two levels of Fermacto feeding for different durations on general metabolic state of broiler chickens by determining the functional and morphological state of liver and kidneys. One hundred and five day-old broiler chicks were randomly divided into seven groups viz A through G each containing fifteen birds. Group A was kept as control. To other six groups Fermacto feeding was done at two different levels (2 gm/kg and 4 gm/kg of feed) and for different durations. The experimental period was 42 days. Organ: body weight indices, FCR, gross and histopathological lesions of liver and kidneys, serum glucose, serum cholesterol, serum total protein and serum uric acid levels and antibody titres against Newcastle disease studied.
The statistical analysis of organ body weight index for left kidney and right kidney showed a highly significant difference between all treated and control groups. The highest mean left kidney body weight index was achieved in the birds of group E. Similarly the highest mean right kidney body weight index was obtained in the birds of group C. Organ: body weight index for liver also showed a highly significant difference between control and treated groups and the highest liver body weight index was achieved in birds of group G. Best feed conversion efficiency was shown by the birds of group B. The gross pathological study of liver and kidneys revealed some nonspecific gross lesions such as slight atrophy, little changes in colour and streaks of haemorrhages in some birds of control and experimental groups. No characteristic histopathological lesion was observed in liver and kidneys of birds except accumulation of RBCs in some birds of control and experimental groups.
The data obtained by the analysis of serum statistically indicated that the concentration of serum glucose and serum cholesterol levels were significantly increased in treated groups than that of control group. Concentration of serum total protein was increased in all treated groups except for C group. But there was no significant difference in serumuric acid levels of all treated and control groups. The data obtained by the analysis of antibody titre against Newcastle disease on zero, 14th, 28th and 42nd day of experiment showed that geometric mean HI titres of all groups carried no significant statistical difference on any of the above mentioned days.
Availability: Items available for loan: UVAS Library [Call number: 0493,T] (1).
83.
Haematological Studies And Estimation Of Electrolytes In Dogs Exhibiting Diarrhoeal Signs
by Shuaib Zaffer, M | Dr. shakeel Akhtar khan | Dr. ahmed Raza | Dr. Asif Rabbani | Faculty of Veterinary Sciences.
Material type: ; Format:
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; Literary form:
not fiction
Publisher: 1997Dissertation note: This project was designed to study the haematological values and the serum electrolytes (sodium, potassium and chloride) levels in the dogs exhibiting diarrhoeal signs. For this purpose one hundred dogs showing diarrhoeal signs were selected from Dog Hospital, College of Veterinary Sciences, Lahore while ten healthy dogs were also used as control group. Tentative diagnosis was tried to establish in each animal of experimental group. Blood samples were collected from the all the animals of both groups. The aematological parameters (TEC, PCV, ESR and Hb) and serum biochemical analysis (sodium, potassium and chloride) were conducted on all blood samples. The changes in blood values of each case was tried to correlate with its tentative diagnosis.
On the basis of tentative diagnosis conditions observed were: parvovirus infection, parasitic infestation, mixed infection, canine distemper and dietary disturbances. These conditions of experimental dogs were named as groups A, B, C, D and E. Haematological values (TEC, PCV, ESR and Hb) in group A were decreased considerably as compared to the control group. In group B the values of (TEC, PCV and ESR) were decreased while the values of Hb was normal as compared to the control group. Group C also showed considerable decrease in the values of haematological parameters. In group D the change in haematological parameters showed that the values of TEC, Hb and PCV were decreased considerably while the value of ESR was increased considerably. The haematological changes in group E were similar to the changes recorded in group E. The changes in biochemical substances revealed that in groups A, B, C and D the mean values of biochemical substances (sodium, potassium and chloride) were decreased as compared to the control group, in group E values of sodium and chloride were decreased slightly while the value of potassium was within the normal range. All the dogs of experimental groups exhibited macrocytic normochromic anaemia.
Availability: Items available for loan: UVAS Library [Call number: 0497,T] (1).
84.
Effect Of Fermacto Feeding On Function And Morphology Of Liver And Kidneys Of Commercial Laying Hens
by Sarwar, M | Dr. Shakil Akhtar khan | Dr. Ahmed Raza | Prof.Dr. Rashid Ahmed Chaudry | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1997Dissertation note: The effect of three levels of Fermacto feeding on egg production, general metabolic state and health of the laying hens was observed by determining the functional and morphological state of liver and kidneys. One hundred, 60 weeks old laying hens, were divided into four equal groups viz A through D each of 25 birds. The hens were already vaccinated. The group A was kept as a control and iii group B, C and D, the Fermacto feeding was done at a dose rate of 1gm, 2gm and 4gm per kg of feed respectively. The experiment period was 60 days. Egg production records, visceral organ: body oveight indices, gross pathological lesions of liver and kidneys, serum glucose, serum cholesterol, serum total protein and serum uric acid levels and antibody titres against Newcastle disease were determined.
The statistical analysis of egg production record showed that from 1-15th day there was no significant difference among the experimental and control groups and from day 16-30th, there was highly significant difference between the experimental and the control groups. The highest mean egg production record was achieved I n the birds of group A. There was no significant difference between the experimental and control groups from day 31-45th ,but there was a significant difference between the experimental and control groups from day 46-60th. The birds from the group B showed the highest mean egg production.
The organ: body weight index for left kidney and right kidney showed a highly significant difference between all experimental and control groups. The highest mean left kidney: body weight index was achieved in the birds of group D. Similarly the highest mean right kidney: body weight index was obtained in the birds of group C. Organ: body weight index for liver also showed a highly significant difference between control and experimental groups and the highest liver: body weight index was achieved in birds of group B. The gross pathological study of liver and kidneys revealed some non-specific gross lesions such as little changes in colour and streaks of haemorrhages in some birds of control and experimental groups. No characteristic pathological lesion was observed in liver and kidneys of birds.
The data obtained by the analysis of serum statistically indicated that the concentration of serum glucose level was significantly increased in experimental groups than that of control group. Concentration of serum cholesterol was also significantly higher in the treated groups than in control group. Concentration of serum total protein was highest in the C group. There was significant difference in the level of serum total protein of all the experimental and control groups. The serum uric acid concentration was significantly different between control and experimental groups. The uric acid concentration was highest in group A. The data obtained by the analysis of antibody titre against ND on 1st, 15th , 30th, 45th and 60th day of the experiment showed that the highest GMHI in the birds of C group which reflected the stimulation of the immune system. So, it was concluded that Fermacto feeding had no deleterious effect on general metabolic state and immune system of laying hens.
Availability: Items available for loan: UVAS Library [Call number: 0498,T] (1).
85.
Changes In The Harderian Gland And Respiratory Tract Of The Broiler Chicken Following Intraocular Vaccination And Infection With/Against Newcastle Disease Virus
by Amer Jumal, M | Dr. Shakil Akhtar Khan | Dr. Ahmad Raza | Dr. M. Asif Rabbani | Faculty of Veterinary Sciences.
Material type: ; Format:
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; Literary form:
not fiction
Publisher: 1997Dissertation note: This project was designed to determine the immune response, macroscopic and microscopic changes in the Harderian gland (HG) and respiratory organs of young chickens reared in well controlled environment following eye-drop vaccination against ND and subsequent challenge with a NDV field strain. This endeavour also represents an effort to raise the curtain on the extent of local and humoral immunity in response to eye-drop vaccination against NDV and also after challenge by two different routes (intraocular and intratracheal).For this project a total of 150 day old broiler chicks were procured from local commercial hatchery. At day one 6 birds were used to determine the maternal antibody titres against ND. On day 7, chicks were randomly subdivided into 6 subgroups Al and A2, Bi and B2, Cl and C2, each subgroup having 24 birds. Three subgroups were vaccinated through eye-dropping against NDV (i.e. Al, A2 and Cl) at 7th and 21st day of age. The other three sub-groups were kept non-vaccinated (i.e. Bi, B2 and C2). Challenge of virulent NDV was administered at 28t1i day of age by two different routes to the different subgroups. Intraocular challenge was given to Al and Bi subgroups and intratracheal challenge was given to A2 and B2 subgroups, whereas Cl and C2 subgroups were kept as control. Determination of antibody response against NDV by heamagglutination inhibition test and gross and histopathology of Harderian glands, trachea and lungs of six birds of all experimental and control subgroups were performed on days 14, 28, 31 and 35 of age. Ocular vaccination engendered higher level of circulating antibodies against NDV but developed poor local protective immunity in tracheal rrucosa. Intratracheal challenge, caused pronounced changes in vaccinateçl birds by eye-dropping in comparison to intraocular challenge, so it can be infered that route of challenge of NDV played a characteristic' role in developing disease to the host vaccinated through eye-droppings. Availability: Items available for loan: UVAS Library [Call number: 0504,T] (1).
86.
Study On Various Mineral Levels And Pathology Of Visceral Organs Of Broiler Chickens Affected With Leg Deformities
by Asim Aslam | Dr. Shakil Akhtar Khan | Dr. Ahmad Raza | Prof. Dr. Rashid Ahmad | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1996Dissertation note: This project was designed to determine the incidence of various leg disorders in commercial broiler chickens and to elucidate the effects of leg deformity on the metabolism of broiler chickens in terms of serum levels of calcium, phosphorus, sodium, potassium, chloride and also on the morphology of liver, kidneys and pancreas. Fifty leg deformed broiler chickens were collected from 38 different commercial poultry farms in and around Lahore, kasur, Sialkot and Gujranwala Districts.
Out of the observed cases of leg deformity, 18 cases of valgus, 13 cases of slipped gastrocnemius tendon, 9 cases of curly toe, 5 cases of femoral head necrosis, 3 cases of varus and only 2 cases of synovitis were reported. This data of incidence of various leg deformaties were analysed on the basis of age, sex, strain of bird and also on the basis of location of deformity. It was noted that the most frequently encountered leg deformity was valgus. The maximum incidence of valgus occurred at 5th week of age. The males have greater incidence of valgus as compared to females. Hubbard strain had greater incidence of valgus in comparison to other strains of birds.
Unilateral cases of valgus were more common than bilateral. The levels of all minerals were decreased in experimental birds as compared to control ones. The mineral levels of birds affected with valgus deformity showed that there was a highly significant difference in the levels of calcium phosphorus and sodium between the experimental and control groups. But there was a non-significant difference in the levels of potassium and chloride in both the groups. The slipped gastrocnemius tendon was noted frequently at 7th week of age, slightly more in females and all the reported cases were unilateral. Hubbard strain was found to be most liable to slipped tendon. The mineral levels of birds affected with slipped tendon showed a highly significant difference in the levels of calcium, phosphorus and potassium between the experimental and control groups. A significant difference was also noted between the experimental and control groups in terms of levels of chloride, but a non-significant difference was recorded between the experimental and control groups in case of serum sodium level. Curly toe was again recorded to its peak in birds of 7 week age, more in males and mostly bilateral. Hubbard strain was found to be the most affected one. The mineral levels of birds affected with curly toe showed a highly significant difference in the levels of calcium and phosphorus between the experimental and control groups. But a non-significant difference was noted between the two groups in cases of serum levels of sodium, chloride, and potassium. Femoral head necrosis was recorded at its highest incidence at 9th week of age, more in males, and all the recorded cases were unilateral. The strain most liable to femoral head necrosis was Indian river. The mineral levels of birds affected with femoral head necrosis showed a highly significant difference in the levels of phosphorus and potassium between experimental and control groups. A significant difference was also recorded between the experimental and control groups in terms of levels of calcium. But in the serum levels of sodium and chloride a non-significant difference was obtained between the two groups. Varus was recorded more frequently at 6 and 7 week of age, more in females, and mostly unilaterally although bilateral cases were also noted. Hubbard, Arboracres, and Lohmann strains showed more susceptible behaviour. The mineral levels of birds affected with varus showed a highly significant difference in the level of calcium. A significant difference was also recorded between the experimental and control groups in terms of levels of phosphorus and potassium, but a non-significant difference was noted in the sodium level between the two groups. Synovitis was noted mostly between 3 and 6 week of age, more or less equally between males and females. Both unilateral and bilateral cases were seen, and Hubbard and Arboracres strains were found to be more prone to synovitis. The mineral levels of birds affected with synovitis showed a significant difference in the levels of calcium and sodium in the experimental and control groups. But a non-significant difference lies between the two groups.
In all these various types of leg deformities, the macroscopic lesions like, slight enlargement of liver along with discolorization and necrotic areas and in some cases congested livers showing the lesions of perihepatitis were also noticed. The kidneys showed inflammatory signs alongwith urates deposits. A pale fibrosed pancreas showed atrophic changes. The pancreas was the most severely affected organ. In majority of the birds it was severely atrophied presenting a shrunken fibrous band like structure in the convoluted duodenal loop. The colour of these pancreases was white or off-white to pink and were firm in consistency. Microscopically cloudy swellings and severe necrosis were seen in the affected livers. Mononuclear and plasma cell infiltration were also quite evident. Leukocytic infiltration in the interstitial spaces and glomerular region were quite evident. The dilated blood vessels and haemorrhages in tissue spaces were observed. In some of the severely affected cases the glomeruli were enlarged and were less in number as compared to that of normal birds.Lesions of necrosis were also noted in some of the affected kidneys. Pancreas was observed that the acinar cells were atrophied and underwent vacuolation, degeneration and necrosis. The Zymogen granules were almost absent from acinar cells. Heterophil, leukocytic and lymphocytic infiltration were frequently encountered in affected cases. A characteristic change was an inflammatory reaction in one or more pancreatic ducts where the epithelium and fibrous tissues occluded the lumen of the ducts and led to obstruction in pancreatic drainage.
Availability: Items available for loan: UVAS Library [Call number: 0513,T] (1).
87.
Comparative Study Of Different Techniques For Diagnosis Of Lymphoid Leucosis In Commercial Layer Chickens
by Azhar Nazir | Dr. Shakil Akhtar Khan | Dr. Ahmad Raza | Dr. Muhammad Sarwar Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1997Dissertation note: This project was designed to study and compare the results of ELISA, impression smears of visceral organs, haematological (TLC and DLC) and histopathological examination for diagnosis of lymphoid leucosis in commercial layers. For this purpose a total of 90 birds were selected from different commercial layer farms, having the history of lymphoid leucosis, age ranged from 5 to 9 months and having enlarged liver and bursa of Fabricious on palpation.
After the selection of birds, their blood was obtained and serum was seprated avoiding the lysis of RBCs. The serum thus obtained was used to detect the lymphoid leucosis antigen with the help of proflock kit of ELISA by KPL USA. Ninety serum samples were put into the ELISA plate well coated with an anti-P27 antibody. The anti-P27 antibody formed a antibody antigen complex. After washing the plate, an affinity purified rabbit anti-P27 peroxidase conjugate was added to each well. The antibody antigen complex remaining from the previous step reacted with the conjugate, brief incubation period was provided and then unreacted conjugate was washed through second wash step. Substrate which contained a chromogen ABTS), was added to each well. Chrornogen colour was changed (from clear to green-blue), which occurred in the presence of peroxidase enzyme. The relative intensity of colour developed in 15 minutes (Compared to control) which is directly proportional to the quantity of P27 antigen in the sample. Now the substrate was incubated, stop solution was added to each well to terminate the reaction. The plate was read using an automatic ELISA reader, which also calculated the SP (sample to positive) values for each sample tested.
The birds having titres of 1000 or above were found positive.
Twenty birds out of total 90 were found positive by ELISA.
These ELISA positive birds were purchased from each farm and the same number of healthy birds from the same flock were subjected to organ body weight ratio and haematological examination (TLC, DLC) and histopathology of positive cases.
The total leukocytic count of the ELISA positive birds showed increase in the leukocytic count when compared with the healthy birds of the same flock. The mean values for ELISA +ve birds of flock number 1, 2, 3 and 4 were 44.93±0.40, 45.90±0.41, 45.90±0.41 and 45.04±0.63, respectively with mean TLC values of 27.32±0.23, 27.38±0.35, 27.38±0.35,27.24±0.29 of healthy birds of flock number 1, 2, 3 and 4, respectively, where the differential leukocytic count showed mild increase in lymphocytes and basophilis and a marked increase in monocyte. The mean value for lymphocytes of the ELISA +ve flock numbers 1, 2, 3 and 4 were 57.35±0.14, 57.30±0.15,57.30±0.15 and 57.76±0.44 respectively compared with the healthy birds of flock numbers 1, 2, 3 and 4 which were 56.0±0.20, 56.0±0.29, 56.20±0.29 and 57.44±0.3. The mean basophilic value for ELISA +ve flock number 1, 2, 3 and 4 were 1.61±0.60, 1.56±0.24, 1.56±0.24 and 1.48±0.37 respectively, compared with the mean value of healthy birds of flock numbers 1, 2, 3 and 4 which were 1.57±0.68, 1.48±0.68, 1.48±0.59 and 1.60±0.37, respectively.
The mean monocyte value for ELISA +ve flock were 14.50±0.13,14.50±0.10, 14.50±0.10 And 14.56±0.44 for flock number 1, 2, 3 and 4, respectively, mean value of the healthy birds were 9.19±0.57, 9.10±0.44, 9.10±0.44, 9.10±0.44 For flock numbers 1, 2, 3 and 4 respectively.
The organ body weight ratio showed a highly significant
increase in the organ body weight indices of affected liver, kidney, spleen, ovary and bursa of Fabricious.
The gross lesions mainly were great enlargement of the liver, spleen, kidney, ovary and bursa of Fabricious, with grayish white, or creamy white foci. In some organ the nodular tumor growth has also seen.
Histopathological studies of the affected birds revealed the infiltration of the lymphoblasts and other blood cells in the affected organ. Areas of necrosis at some places in affected organ were observed. The tumors cells showed the aggrigates of lymphoblast. The impression smears made from the fresh specimen of liver, spleen, kidney, ovary and bursa of Fabricious, stained with Giemsa stain, confirmed the lymphoid leucosis due to the presence of lymphoblast.
Out of 20 positive cases, liver was affected in all the cases, spleen in 18 cases, ovary in 15 cases, kidney in 11 and bursa of Fabricious in 14 cases.
It was concluded that ELISA can be used as a most reliable and effective method of diagnosis in live birds, whereas in dead birds, disease can be confirmed by the presence of lymphoblasts in impression smears of liver and spleen. Blood changes can be use as aid in early diagnosis, whereas histopathology is a time consuming procedure.
Availability: Items available for loan: UVAS Library [Call number: 0514,T] (1).
88.
Studies On The Incidence And Pathology Of Different Reproductive Disorders In Desi Laying Hens In And Around Lahore
by Shahbaz ul Haq | Dr. Shakil Akhtar Khan | Dr. Asim Aslam | Dr. Muhammad Aleem | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1997Dissertation note: This project was designed to study incidence and pathology of reproductive disorders in Desi laying hens. This study also represented an endeavour to establish relationship between salmonellosis, mycoplasmosis and egg drop syndrome-76 infections and diverse types of reproductive abnormalities. A total of 300 Desi laying hens were randomly selected from the various poultry sale centres of Lahore and villages around Lahore. Blood sample and female genital tract of each bird were collected. The ovaries and oviducts of all hens were thoroughly examined to detect any gross pathological lesion. Organs exhibiting gross pathological lesions were subjected to histopathological examination. The biometrical study of ovaries and oviducts of all birds was also conducted. Serologically the incidence of salmonellosis, mycoplasmosis and egg drop syndrome 76 was detected by adopting rapid method agglutination test, rapid slide agglutination test and haemagglutination inhibition test, respectively.
In this study 32 (10.67%) birds exhibited five pathological conditions i.e. oophoritis, degenerated ovarian follicles, salpingitis, cystic oviduct and adenoma. Macro and microscopic pathological lesions were studied in all the above five conditions. Out of 300 serum samples, 113 (37.66%) were positive for Salmonella pullorum and Salmonella gallinarum infection, 209 (69.66%) were positive for Mycoplasma gallisepticum infection and 41 (13.66%) samples were positive for EDS-76 infection.
Availability: Items available for loan: UVAS Library [Call number: 0517,T] (1).
89.
Comparative Study On The Effects Of Dietary Aflatoxin And Detoxified Aflatoxin On Functions Of Liver In Commercial
by Kamran Younas Chohan | Dr. Shakil Akhtar Khan | Dr. Ahmad Raza | Dr. Talat Naseer Pasha | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1997Dissertation note: The effect of dietary aflatoxin and detoxified aflatoxin was studied on postmortem lesion, total serum protein, serum bilirubin, SGOT and SGPT in commercial broiler, which were fed on broiler starter ration upto 21 days. From 21st day to 42nd day they were divided into 5 group and fed toxicated feed along with different treatment. Aflatoxins were produced on corn by moistened it at room temperature for 15 days, and the concentration of aflatoxin in corn was determined through direct ELISA (Brinton, 1987) from a commercial Laboratory. This corn were ground and appropriate amount of this was mixed into broiler finisher ration to generate 70 ppb aflatoxin B1 level.
One hundred day old broiler chicks were obtained from a commercial hatchery and fed on broiler starter ration upto 21 days. At 21st day these were randomly divided into 5 groups, having 20 birds each. Each birds in each. One group was received simple feed, 2nd group was received contaminated feed with aflatoxin B1 70 ppb, 3rd, 4th, and 5th group were received contaminated feed with 70 ppb aflatoxin and treated for detoxification with extrusion, sodium bentonite and Mycofix plus inclusion respectively.
All the birds were thoroughly observed throughout the experimental period to detect any clinical sign deviated from normal. All the birds of each group were slaughtered at the end of experiment at 42nd days their blood was taken for separation of serum and gross postmortem lesions were recorded.
The common clinical symptoms observed in chicken at 70 ppb level were dullness, depression, paleness of combs, wattles and shank. The severity of these symptoms were less in groups feed a diet contaminated with aflatoxin with detoxification with extrusion and Mycofix plus. The group fed diet containing sodium bentonite, normal combs, wattles and shanks. Birds were remained active.
The postmortem changes in the group receiving 70 ppb dietary aflatoxin showed the enlargement of liver, kidney, pancreas and spleen. Haemorrhages were present on pancreas and on intestine. The severity of lesion on these organs were less in groups fed on diet contaminated with aflatoxin with detoxification with extrusion, Mycofix plus and sodium bentonite. The group fed on sodium bentonite added contaminated diet has normal intestine.
The total serum protein in group 2 decreased at 70 ppb aflatoxin level in feed. In group 3 which was treated with extrusion serum protein level also decreased. In group which was treated with sodium bentonite protein level increased and showed much improvement. In group 5 it also increased but less than group 4. Increase was highly significant (P<0.01) at the end of experiment.
The total serum bilirubin in group 2 increased at 70 ppb aflatoxin level. Improvement was in group 3,5 and 4 which were treated with extrusion, Mycofix plus and sodium bentonite. Much improvement in group 3 whose feed was detoxified with extrusion process. Decrease was highly significant (P<0.01) at the end of experiment.
The activity of SGOT in group 2 were increased at 70 ppb aflatoxin level in feed as compared to control. The activity of SGOT in treated groups were also increased. The treatment means of SGOT differed significantly (P<0.01).
The activity of SGPT in group 2 was increased at 70 ppb aflatoxin level in feed as compared to control. The activity of SGPT is treated group were also increased. The treatment means of SGPT differed significantly (P<0.01).
It was concluded from this study that the aflatoxin contaminated feed can be detoxified with extrusion, Mycofix plus or with addition of sodium bentonite. Addition of these in the feed improved the general body condition and liver function of the chickens.
Availability: Items available for loan: UVAS Library [Call number: 0521,T] (1).
90.
Classification Of Anaemia In Dogs
by Farid Khan | Dr. Javed Rashid | Dr. Aasif | Dr. Shakil Akhtar Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1997Dissertation note: The present project was designed to investigate various causes and the incidence rate of anaemia and further to classify it on the basis of morphological changes.
Forty dogs were used brought to the Dog Hospital, College of Veterinary Sciences, Lahore. Based on history and clinical examination the dogs were described as anaemic. Fifteen dogs were used as control animals. Haemoglobin (Hb) estimation, packed cell volume (PCV), total erythrocytic count (TEC) and erythrocyte indices (mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration) were investigated.
Among forty sick dogs fourteen were found anaemic due to nutritional deficiency, nine due to parvovirus infection, seven were suffering from parasitic infestation, seven had mixed infection and only three had canine distemper. High incidence of anaemia indicated nutritional deficiency.
It is concluded that anaemia in dogs due to parvo virus infection, parasitic infestation and canine distemper is of macrocytic normochromic, due to mixed infections macrocytic hypochromic and nutritional deficiency had microcytic normochromic, normocytic normochromic, normocytic hypochromic and microcytic hypochromic in nature.
Availability: Items available for loan: UVAS Library [Call number: 0528,T] (1).
91.
A Study On The Effect Of Doxin On The Immune System And Its Toxicity In Broiler Chickens
by Afzal, M | Dr. Shakil akhtar Khan | Dr. Khlid Muneer | Dr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1997Dissertation note: The project was designed to study the effect of Doxin on the 1.txuxuine system and if any, its toxicity (in normal and excessive doses) in broiler chickens. A total number of' 150 day old broJer chicks were randomly divided into six equal groups, A through F, each containing 25 birds. Group A arid B were kept as control i.e. A was non-medicated (Doxin) and vaccinated against ND while B was non-medicated (Doxin) and non vaccinated against ND. Groups C and E were medicated I)oxin at different levels (0.5gm/I and 4 gm/I) at 1st to 5th day and 22nd to 26th day respectively. Both groups were vaccinated against ND. Groups D and F were medicated Doxin at different levels (0.5gm/i and 4 gm/i) at 1st to 5th day and 22nd to 26th day respectively, both groups were non vaccinated against ND. The experimental period was 42 days. Organ body weight indices, gross lesions on liver, spleen, thymus, bursa of Fabricius and kidneys, determination ol immune response against Newcastle disease, serum total protein, albumin, globulin, A/G ratio, alkaline phosphatase, alanine amino transferase, aspaitate amino transferase, uric acid and urea levels were conducted.
The statistical analysis of organ body weight index for liver showed significant difference between the medicated and nonmedicated (control) groups. The highest. liver body weight index was achieved in the birds of group E. The organ body weight indices of spleen, thymus and bursa of Fabricius are quite irregular. The data obtained by the the analysis of serum statistically indicated that the mean levels of serum total protein and albumin of control and medicated groups were not significantly different from each other. The mean levels of Globulin, A/G ratio of vaccinated and non- vaccinated groups carry statistical significant difference. There was no significant difference in alkaline phosphatase (ALP), alanine amino transferase (ALT) and asparate amino transferase (AST) levels of all medicated and non-medicated groups. The mean levels of urea and uric acid were increased in the medicated groups, highest in the high dose medication. The data obtained by the analysis of antibody titre against Newcastle disease on 0, 14th, 28th and 42nd day of experiment showed that geometric mean HI titres of all medicated groups decreased that of control groups.
Availability: Items available for loan: UVAS Library [Call number: 0532,T] (1).
92.
Effects Of A Live Coccidial Vaccine On Browth Traits And Immune Response In Broiler Chickens
by Azam Ali Nasir | Dr. Javed Rashed | Dr. Asif | Dr. Shakil Akhtar Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1997Dissertation note: One hundred day old broiler chicks at the age of two days were randomly divided into four groups A, B, C and D. Group B, C and D were vaccinated orally with Cocci-vac on 3rd, 10th and 20th day of age respectively, while group A was kept as non-vaccinated control. At the age of 35 days, all the birds of each group were challenged with coccidial oocysts and the effects of Cocci-vac in controlling coccidiosis in broiler chicks as well as its effects on immune response and growth traits were studied.
The immunogenic effects of Cocci-vac were recorded by challenging the birds with sporulated oocysts. In vaccinated groups (B, C and D) the immunity was better than non-vaccinated control group (A), as there were reduced or no signs of coccidiosis in vaccinated groups. The data obtained from oocysts count per gram of feces showed that the number of oocysts was 28290 and 33830 in group A (control) and 14685 and 16480 in group B (vaccinated) on 7th and 10th day post challenge, while group C had not shown any oocysts count throughout the experimental period. However, group D had shown oocysts count 3240 on 10th day post challenge but not on 7th day. The data obtained from analysis of antibody titers against Newcastle disease on 14th, 28th and 42nd day of experiment, revealed that the GM HI titers of group A (control) was slightly better (181 and 119.4) than other groups B (168.9 and 104), C (104 and 78.8) and group D (168.9 and 55.7) on 14th and 28th day but the lowest (9.8) in group A on 42nd day than other groups B (14.9), C (19.7) and D (13.9). The gross pathological study of intestine showed serosal changes (inflammation and haemorrhages) and mucosal changes (thickened wall, haemorrhages and blood tinged contents), while liver showed discolouration. These changes were much prominent in group A as compared to group B. Spleen and bursa of Fabricius represented no lesions. The data obtained by analysis of weight gain and FCR showed better weight gain (1614, 1783 and 1720) in groups B, C and D respectively and FCR 2.35, 2.24 and 2.26 in groups B, C and D respectively, in vaccinated groups as compared to control .group 'A' (Weight gain 1301 and FCR 2.45). However, within the vaccinated group C showed the best results.
Availability: Items available for loan: UVAS Library [Call number: 0538,T] (1).
93.
Effect Of Two Anticoccidials With And Without Supplementation Of Vitamins On Some Liver And Kidney Functions
by Muti Ur Rehman Khan | Dr. Shakil Akhtar Khan | Dr.Javed Rashid | Dr.Muhammad Amin Sheikh | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1998Dissertation note: The project was designed to study the effect of anticoccidials (Ampisol 20% and Esb3 30%) with and without supplementation of vitamins on some liver and kidney functions of broiler chicken. A total of hundred day old broiler chicks were randomly divided into five equal groups designated as A, B, C, D and E. Group A was medicated with Ampisol 20% at the dose rate of 3 gm/10 liters. Group B was given Ampisol 20% alongwith supplementation of Symodek at the dose rate of 1 gmJ2 liters. Group C was medicated with Esb3 30% while group D was Esb3 30% plus Symodek. Group E was non- medicated control. Serum samples were collected randomly from five birds in each group on day 21, 28, 35 and 42 and were stored at -20°C uptil tests. The experimental period was 42 days. Estimation of alanine aminotransferase, aspartate aminotransferase, serum creatinine and serum uric acid was made by using commercially available kits in collected serum samples of all groups.
The statistical analysis of alanine aminotransferase, aspartate aminotransferase, serum creatinine showed no significant difference among groups A, B, C, D and Eon 21, 28,35,42 day of experiment. Serum uric acid levels showed no significant difference in different groups on 21 and 42 day. On day 28 of the experiment there was no significant difference between mean serum uric acid levels of group C and D but were significantly different from groups A, B and E. The mean uric acid levels of groups A, B and E were significantly different from each other. On day 35 there was significant difference among different groups except between B and C groups. As there were no adverse effects on liver and kidney functions of broiler chickens, the administration of Ampisol 20% and Esb3 30% with and without vitamins is recommended for chemoprophylaxis of coccidiosis in broiler chickens.
Availability: Items available for loan: UVAS Library [Call number: 0576,T] (1).
94.
Effects Of Two Anticoccidial Drugs With And Without Supplementation Of Vitamins On The Immune System Of Broiler Chickens
by Nazim ud Din | Dr.Khalid Munir Ch | Dr.Muhammad | Dr.Shakil Akhtar Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1998Dissertation note: This project was designed to investigate the effects of two anticoccidials Ampisol 20% and Esb3 30% with and without supplementation of vitamins (A, D3, E and K3 as "Symodek") on the immune system of broiler chickens. To evaluate such effects the parameters used for study were; effects, on the weights of immuno regulatory organs (bursa of Fabricius, thymus, spleen and liver), organ body weight indices, histopathology of primary lymphoid organs (bursa of Fabricius and thymus), total and differential leukocytic counts, total serum protein, albumin globulin ratio and immune response to Newcastle disease virus vaccination. A total of 120 chicks, divided into 5 equal groups viz A to E were reared for 42 days. Groups A, B, C, and D were medicated with, Ampisol 20%, Ampisol 20% + Symodek, Esb3 30%, and Esb3 30% + Symodek, respectively while group E was kept as control(no drug medication). All the birds were vaccinated with ND virus vaccine on day 7th and day 2 1st. All the medication, was done through drinking water at the recommended prophylactic dosage levels.
The study revealed that Ampisol 20% had favourable effects on the weights of immunoregulatory organs, and organ (bursa of Fabricius, thymus, spleen and liver):bodyweight indices. Esb3 30% had negative effects on these parameters.
Histological studies of primary lymphoid organs (bursa of Fabricius and thymus) revealed no detrimental effects of different medications except in birds of group C and D given Esb3 30% or Esb3 30% + Symodek, where slight degenerative changes were noted in bursae and thymi.
On day 42 the mean TLC values were slightly lower in birds medicated with Esb3 30% as compared to birds which received Ampisol 20%. On day 42 the mean lymphocytic percentages were higher in groups medicated with Arnpisol 20% as compared to those given Esb3 30%. Vitamins supplementation exerted favourable effects on TLC and DLC values. This study further indicated that mean total serum protein (TSP), mean serum albumin, mean serum globulin and mean A/G ratios were higher in birds medicated Ampisol 20%, than those medicated Esb3 30%. Vitamin supplementation had augmenting effects on these values. On day 28, 35 and 42 the antibody titres in birds medicated with Ampisol 20% were higher than those given Esb3 30%. Vitamin supplementation exerted augmenting effects on the antibody titres.
It is concluded that Ampisol 20% had no immunosuppressive effects rather it exerted favourable effects on the weights of immunoregulatory organs, rpp albumin, globulin, A/G ratio, DLC and HI antibody titres against Newcastle disease virus. Esb3 30% moderately depressed the aforesaid components of immune system. The present study also revealed that vitamins (A, D3, E, K3) supplementation to anticoccidials improved the immune response of birds. However, there is a need for further investigation in this regard, with special reference to immune system of broiler chickens.
Availability: Items available for loan: UVAS Library [Call number: 0579,T] (1).
95.
Effects Of Formalinized And Oil Based Hydropericardium Syndrome Vaccines On Haematology & Immunocompetent
by Zafar Abbas | Dr.Javed Rashid | Dr.Muhammad | Dr.Shakil Akhtar Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1998Dissertation note: This study was designed to find out the effects on haematology (Hb, TLC and DLC), immune response (against NDV vaccine) and on organ body weight indices (liver, spleen, bursa of Fabricius and thymus) in broiler chickens when given two different HPS vaccines (Formalinized and oil-based HPS vaccines). Morbid livers of chickens affected with naturally occurred hydropericardium syndrome (HPS) were collected from field outbreaks to prepare the HPS vaccines and inoculum. Sixty broiler chicks were divided into three groups (A, B and C), comprising of 20 chicks in each on 10th day of experiment. At 14th day of age, chickens of groups A and B were given f-HPS and o-HPS vaccines, while group C was kept as unvaccinated control. On 28th day of age chickens of groups A, B and C were challenged with HPS inoculum. To study the effects of f-HPS and o-HPS vaccines, blood samples were collected on 12th (pre-vaccination), 26th (postvaccination) and 35th (post-challenge) day of experiment.
Haematological study revealed that f-HPS and o-HPS vaccines had no significant effects on haemoglobin concentration in groups A and B, but its concentration decreased significantly on 35th day in control group-C. Whereas TLC values increased significantly on 26th and 35th day in groups A and B, but decreased significantly on 35th day in group-C. DLC value revealed that there was relative lymphocytosis and heterpenia on 26th and 35th day in groups A and B, whereas eosinophilia on 35th day. While in control group-C there was lymphopenia and eosinopenia along with hetrophHia on 35th day. Antibody titre against Newcastle disease virus vaccine indicated that there was no significant difference in'GMHI titre values among the groups A, B and C. Results of organ body weight indices indicated that in control group-C (unvaccinated) indices of liver, spleen and bursa of Fabricius were significantly higher than those of groups A and B, whereas no effect was observed on thymic body weight index among groups A, B and C.
The results of this study suggested that f-HPS and o-HPS vaccines had no significant effect on Hb concentration, TLC and DLC values. No significant immuno-modulatory effect was noted on antibody titre against NDV vaccine of these HPS vaccines, whereas significant effect was noted on organ body weight indices of livers, spleen and bursa of Fabricius in group-C as compared to vaccinated groups.
Availability: Items available for loan: UVAS Library [Call number: 0582,T] (1).
96.
Studies On Stress Induced Effects In Broilers Following Vaccination (Nobilis Ma5 + Clone 30) Against Infectious Bronchitis and Newcastle Disease
by Saima Irum Syed | Dr.Asim Aslam | Dr.Sameera | Dr.Shakil Akhtar Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2000Dissertation note: This study was intended to detect the stress induced by live, freezed-dried vaccine against Newcastle disease and infectious bronchitis disease viruses in broilers and to determine the comparative efficacy of vitamins and aspirin to combat it. One hundred and sixty day-old chicks were divided into four group i.e. A, B, C and D having 40 birds each. The birds were kept for 42 days after vaccination with Nobilis Ma5+Clone 30 by eye-droppings on 1st and 22nd day of age. Chicks from group A was kept as control. Group B was given vaccine and no treatment. Group C was given vaccine and only vitamins (Vety Stress-Check). Group D was given vaccine and only aspirin. Ten birds from all groups were randomly selected to collect the serum samples from blood on 1st, 14th, 28th and 42nd day of antibody titre determination.
The following parameters were studied (1) antibody response against Newcastle disease vaccine, (ii) Serum bio-chemical substances (Total serum protein, glucose and cholesterol), (iii) Heterophil/ lymphocyte ratio, (iv) Adrenal/body weight ratio (v) Gross histopathology of adrenal glands and (vi) the economics of the flock.
The live virus Newcastle disease vaccine had no significant stressor effect on any of the above parameters. However, use of vitamins played vital role in combating the vaccination stress, because it showed immuno competent effect and also positive trend in growth traits. That is why this group showed better economic value than the rest of the groups. The effect of Aspirin was also variable.
Availability: Items available for loan: UVAS Library [Call number: 0653,T] (1).
97.
Study Of Incidence & Pathology Of Different Reproductive Disorders In Female Breeder Layers
by Imtiaz Ahmed Cheema | Dr. Shakil Akhtar Khan | Dr. Asim Aslam | Dr.Rashid Ahmad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2000Dissertation note: This study was carried out to find the incidence and pathology of reproductive disorders in breeder female layers. A total of 120 breeder female layers were randomly selected from various poultry sale centers of Lahore. Blood sample and female genital tract of each bird were collected. The ovaries and oviducts of all hens were thoroughly examined to detect any gross pathological lesion. Organs exhibiting gross pathological lesions were subjected to histopathological examination. The biometrical study of ovaries and oviducts of all birds was also conducted. Serologically, the incidence of Salmonella pullorum and Mycoplasma gallisepticurn was detected by adopting rapid method agglutination test and rapid slide agglutination test, respectively. This study also represented an endeavor to establish relationship between Salmonellosis and Mycoplasmosis infections and adverse types of reproductive abnormalities.
In this study 15 (12.5 %) birds exhibited seven pathological conditions i.e. cystic ovary, degenerated ovarian follicles, salpingitis, impaction of oviduct, ruptured oviduct, adenoma and underdeveloped tracts. Macro and microscopic pathological lesions were studied in all the above conditions. Out of 120 serum samples 57 ( 47.5 % ) were positive for Salmonella pullorum infection and 87 (72.5%) were positive for Mycoplasma gallisepticum infection.
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98.
A Study On Stress Induced Effects In Broilers After Vaccination (Angavag) Against Hydropericadium Syndrome
by Muhammad Yasin Tipu | Dr. Shakil Akhtar Khan | Dr.Asim Aslam | Dr.Muhammad Aslam | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2000Dissertation note: The main aim of this study was evaluation of vaccination (Angavac) stress against hydropericardium syndrome with an oil- based vaccine and possible measures to check that stress. For this purpose one hundred and seventy chicks (day-old) were randomly divided into four groups namely, A, B, C and D. Each group comprised of forty birds while ten birds were used to collect serum samples for evaluation of maternal antibody titre in the chicks. Group "A" was kept as control while group B, C and D were vaccinated against Hydropericardium syndrome (HPS). Group "B" was kept as such while group C and D were supplemented with multi-vitamins and aspirin respectively in drinking water. Blood samples were collected at 24, 48 and 72 hours post vaccination from ten randomly selected birds from each group to study heterophil/ lymphocyte ratio and change in serum chemistry i.e. (protein, glucose and cholesterol). Blood serum was also collected from 10 randomly selected birds of each group an day 14, 28 and 42 of the experiment to see the level of antibody titre in the groups. Adrenal/body weight ratio, pathological examination of adrenal gland and economics of the flock was also studied at the end of experiment. Vaccine did not cause any significant stress in broilers. However, multivitamin therapy gave better growth traits and increased profit.
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99.
Detection Of Stress Following Vaccination (Eimeria Vaccine) Against Coccidiosis In Broilers
by Asghar Ali | Dr.Asim Aslam | Dr.Shakil Akhtar Khan | Haji Ahmad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2000Dissertation note: The present project was designed to detect the stress following vaccination (Eimeria Vaccine) against coccidiosis in broilers and its management by using multivitamins and aspirin. In this study a total of 160 day old broiler chicks were randomly divided into four groups designated as A, B, C and D Group A was kept as non vaccinated, non treated control and group B was vaccinated against coccidiosis but no other treatment was given. While group C and D were vaccinated against coccidiosis and treated with multivitamins vitamins and aspirin respectively.
The parameters studied were heterophil/lymnphocyte ratio, total serum protein, serum glucose, serum cholesterol estimation, for three consecutive post vaccination days. At the end of experiment, on 42nd day, adrenal gland body weight ratio and histopatholor of adrenal gland was performed to assess any change.
In our experiment there was no statistical significant difference among different groups. 1-lowever, group C which was given multivitamins showed maximum weight gain and minimum stress, while the aspirin therapy to the group D did not show any difference with group A and B.
In the adrenal body weight ratio, there was also no significant difference among different groups. No gross and histopathological changes were seen in any group.
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100.
Management Of Vaccinal (Nobilis Gumboro D-78) Stress In Broiler Chicks
by Amir Qadeer Nizami | Dr.Asim Aslam | Dr.Kamran | Dr.Shakil Akhtar Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2000Dissertation note: This project was planned to know the effects of vaccinal stress by infectious bursal disease (IBD) live virus vaccine (D-78) strain and to determine the methods by certain compounds to overcome the stress. Three hundred and thirty (330) day-old birds were reared upto 42 days. Ten birds out of 330 were slaughtered at zero day of the experiment, for estimation of maternal antibody titre. The remaining 320 birds were randomly divided into four equal groups i.e., A, B, C and D. Group A was kept as control. Group B was vaccinated but non-medicated, Group C was vaccinated and treated with vitamins for three post-vaccination days and group D was vaccinated and treated with aspirin for three post-vaccination days. Blood samples were collected on days 14th, 28th and 42nd for detection of antibody titer against IBD. Blood samples were collected from 10 randomly selected birds of each group after 1st, 2nd, 3rd and 4th day postvaccination. In this project following parameters were studied (i) estimation of serum biochemical substances, (ii) heterophil/ lymphocyte ratio, (iii) estimation of antibody response against IBD vaccine (iv) adrenal gland body weight index, (v) pathological studies
of adrenal glands and (vi) economics of the flock.
It was analyzed whether this management was an economical procedure or not by estimating the expenses, on raising the management of these stressed birds with aspirin and vitamin. It was estimated that aspirin and vitamin both check the stress to a certain extent. The birds expressed vaccination stress for variable period ranging from 1-2 days post-vaccination. In field conditions whether the flock is small or large aspirin and vitamins can be therapeutically given to control the stress and these two agents are not expensive, so they do not have any marked effect on economics of any flock.
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