1.
Studies On Stress Induced Effects In Broilers Following Vaccination (Nobilis Ma5 + Clone 30) Against Infectious Bronchitis and Newcastle Disease
by Saima Irum Syed | Dr.Asim Aslam | Dr.Sameera | Dr.Shakil Akhtar Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2000Dissertation note: This study was intended to detect the stress induced by live, freezed-dried vaccine against Newcastle disease and infectious bronchitis disease viruses in broilers and to determine the comparative efficacy of vitamins and aspirin to combat it. One hundred and sixty day-old chicks were divided into four group i.e. A, B, C and D having 40 birds each. The birds were kept for 42 days after vaccination with Nobilis Ma5+Clone 30 by eye-droppings on 1st and 22nd day of age. Chicks from group A was kept as control. Group B was given vaccine and no treatment. Group C was given vaccine and only vitamins (Vety Stress-Check). Group D was given vaccine and only aspirin. Ten birds from all groups were randomly selected to collect the serum samples from blood on 1st, 14th, 28th and 42nd day of antibody titre determination.
The following parameters were studied (1) antibody response against Newcastle disease vaccine, (ii) Serum bio-chemical substances (Total serum protein, glucose and cholesterol), (iii) Heterophil/ lymphocyte ratio, (iv) Adrenal/body weight ratio (v) Gross histopathology of adrenal glands and (vi) the economics of the flock.
The live virus Newcastle disease vaccine had no significant stressor effect on any of the above parameters. However, use of vitamins played vital role in combating the vaccination stress, because it showed immuno competent effect and also positive trend in growth traits. That is why this group showed better economic value than the rest of the groups. The effect of Aspirin was also variable.
Availability: Items available for loan: UVAS Library [Call number: 0653,T] (1).
2.
A Study On Stress Induced Effects In Broilers After Vaccination (Angavag) Against Hydropericadium Syndrome
by Muhammad Yasin Tipu | Dr. Shakil Akhtar Khan | Dr.Asim Aslam | Dr.Muhammad Aslam | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2000Dissertation note: The main aim of this study was evaluation of vaccination (Angavac) stress against hydropericardium syndrome with an oil- based vaccine and possible measures to check that stress. For this purpose one hundred and seventy chicks (day-old) were randomly divided into four groups namely, A, B, C and D. Each group comprised of forty birds while ten birds were used to collect serum samples for evaluation of maternal antibody titre in the chicks. Group "A" was kept as control while group B, C and D were vaccinated against Hydropericardium syndrome (HPS). Group "B" was kept as such while group C and D were supplemented with multi-vitamins and aspirin respectively in drinking water. Blood samples were collected at 24, 48 and 72 hours post vaccination from ten randomly selected birds from each group to study heterophil/ lymphocyte ratio and change in serum chemistry i.e. (protein, glucose and cholesterol). Blood serum was also collected from 10 randomly selected birds of each group an day 14, 28 and 42 of the experiment to see the level of antibody titre in the groups. Adrenal/body weight ratio, pathological examination of adrenal gland and economics of the flock was also studied at the end of experiment. Vaccine did not cause any significant stress in broilers. However, multivitamin therapy gave better growth traits and increased profit.
Availability: Items available for loan: UVAS Library [Call number: 0656,T] (1).
3.
Detection Of Stress Following Vaccination (Eimeria Vaccine) Against Coccidiosis In Broilers
by Asghar Ali | Dr.Asim Aslam | Dr.Shakil Akhtar Khan | Haji Ahmad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2000Dissertation note: The present project was designed to detect the stress following vaccination (Eimeria Vaccine) against coccidiosis in broilers and its management by using multivitamins and aspirin. In this study a total of 160 day old broiler chicks were randomly divided into four groups designated as A, B, C and D Group A was kept as non vaccinated, non treated control and group B was vaccinated against coccidiosis but no other treatment was given. While group C and D were vaccinated against coccidiosis and treated with multivitamins vitamins and aspirin respectively.
The parameters studied were heterophil/lymnphocyte ratio, total serum protein, serum glucose, serum cholesterol estimation, for three consecutive post vaccination days. At the end of experiment, on 42nd day, adrenal gland body weight ratio and histopatholor of adrenal gland was performed to assess any change.
In our experiment there was no statistical significant difference among different groups. 1-lowever, group C which was given multivitamins showed maximum weight gain and minimum stress, while the aspirin therapy to the group D did not show any difference with group A and B.
In the adrenal body weight ratio, there was also no significant difference among different groups. No gross and histopathological changes were seen in any group.
Availability: Items available for loan: UVAS Library [Call number: 0662,T] (1).
4.
Management Of Vaccinal (Nobilis Gumboro D-78) Stress In Broiler Chicks
by Amir Qadeer Nizami | Dr.Asim Aslam | Dr.Kamran | Dr.Shakil Akhtar Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2000Dissertation note: This project was planned to know the effects of vaccinal stress by infectious bursal disease (IBD) live virus vaccine (D-78) strain and to determine the methods by certain compounds to overcome the stress. Three hundred and thirty (330) day-old birds were reared upto 42 days. Ten birds out of 330 were slaughtered at zero day of the experiment, for estimation of maternal antibody titre. The remaining 320 birds were randomly divided into four equal groups i.e., A, B, C and D. Group A was kept as control. Group B was vaccinated but non-medicated, Group C was vaccinated and treated with vitamins for three post-vaccination days and group D was vaccinated and treated with aspirin for three post-vaccination days. Blood samples were collected on days 14th, 28th and 42nd for detection of antibody titer against IBD. Blood samples were collected from 10 randomly selected birds of each group after 1st, 2nd, 3rd and 4th day postvaccination. In this project following parameters were studied (i) estimation of serum biochemical substances, (ii) heterophil/ lymphocyte ratio, (iii) estimation of antibody response against IBD vaccine (iv) adrenal gland body weight index, (v) pathological studies
of adrenal glands and (vi) economics of the flock.
It was analyzed whether this management was an economical procedure or not by estimating the expenses, on raising the management of these stressed birds with aspirin and vitamin. It was estimated that aspirin and vitamin both check the stress to a certain extent. The birds expressed vaccination stress for variable period ranging from 1-2 days post-vaccination. In field conditions whether the flock is small or large aspirin and vitamins can be therapeutically given to control the stress and these two agents are not expensive, so they do not have any marked effect on economics of any flock.
Availability: Items available for loan: UVAS Library [Call number: 0663,T] (1).
5.
Effects Of Stress Folowing Vaccination (Avinew) Against Newcastle Disease In Broilers
by Asaf Jamal Khan, Major M | Dr.Asim Aslam | Dr.Irshad | Dr.Sohail Akhtar Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2000Dissertation note: This experiment was designed to find out the degree of stress and immune status in response to vaccination (Avinew) against ND and also to know the effects of two therapeutic agents, i.e. multivitaminsand aspirin, on efficacy of the vaccine. One hundred and sixty day old broiler chicks were divided into four groups i.e. A, B, C and D, each group having 40 birds. Group A was kept as control, B was vaccinated but non-medicated, group C was given multivitamins alongwith vaccine for 3 post-vaccination days and group D was treated with aspirin for 3 post-vaccination days. Blood samples were collected on days 1st, 14th, 28th and 42nd for detection of antibody titer against NDV. Blood samples were collected from 10 randomly selected birds of each group after 24, 48 and 72 hours of vaccination for study of heterophil/lymphocyte ratio and estimation of serum biochemical substances (protein, glucose and cholesterol). On 42nd day of the experiment, adrenal glands of 10 randomly selected birds from each group were examined for adrenal/body weight ratio and gross/histopathological lesions. At the end of the experiment the economics of the flocks was also calculated. "AVINEW" (freezed dried live VG/GA vaccine) against Newcastle disease, activated the immune response of the chicks without any significant stress. It provided a reasonable and persistent antibody titer. Multivitamins supplementation showed immuno-potentiating effect and also positive trend in growth traits. The effect of aspirin on immune response and weight gain was variable.
Availability: Items available for loan: UVAS Library [Call number: 0674,T] (1).
6.
Studies On Stress Induced Effects In Broilers Following Vaccination (Hydro Poultry) Against Hydropericadium
by Gulbeena Saleem | Dr. Shakil Akhtar Khan | Dr.Asim Aslam | Dr.Sameera Akhtar | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2000Dissertation note: The project was aimed at determining the vaccination stress follow hydropericardium syndrome vaccine (hydro-poultry vaccine, simple vaccine; Adenovirus type-4 Pak) by subcutaneous route under wing at 12th day of age and comparative efficacy of multivitamins and aspirin to combat it. One hundred and seventy day old broiler chicks were divided into four groups i.e. A, B, C and D having 40 birds each. Ten-day old broiler chicks were used for collection of blood sample by cardiac puncture for detection of maternal antibody titre. Birds were kept for the period of 42 days. The birds of group A served as unvaccinated, unchallenged control. Group B was given vaccine and no treatment. Group C was given vaccine and multivitamins for 3 days post-vaccination group D was given vaccine and aspirin for 3 days post vaccination. Blood samples were collected from 10 randomly selected birds from each group at different post vaccination (Against HPS) periods i.e. 24, 48 and 72 hrs for serum biochemical analysis and 10 birds from each group at 14th 28th ad 42' days for determining antibody response against hydropericardium syndrome. 10 birds of each group were slaughtered on 42 days for collection of adrenal glands. The following parameters were studied (1) determination of heterophil/lymphocyte ratio (ii) estimation of serum biochemical analysis (Total Serum Protein, serum glucose, serum cholesterol). (iii) determination of adrenal body weight ratio (iv) gross pathological and histopathological examination of adrenal glands (v) estimation of antibody response against hydropericardi um syndrome vaccine. Inactivated virus vaccine against hydropericardium was unable to induce any stress in broiler chicks and the above mentioned parameters can act as good indicators for detection of vaccination stress to some extent. Multivitamins played a minor role in improving the condition of bird whereas, aspirin did not seems to be much effective in this study.
Availability: Items available for loan: UVAS Library [Call number: 0707,T] (1).
7.
Detection And Control Of Vaccination Stress In Pullets Against Newcastle Disease Vaccine (Avinew)
by Shakila Khanum | Dr. Shakil Akhtar Khan | Dr.Asim Aslam | Dr.Muhammad Akram Munir | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2000Dissertation note: The project was carried out to detect the stress in layer chicks caused by vaccination against Newcastle disease. It alsi intended to find out the role of vitamins and aspirin in combating vaccination stress. One hundred and seventy (170) day-old layer chicks were obtained from the market. 10 day old chicks were utilized to determine maternal antibody Litre against NDV. Four groups were made at random comprising of 40 birds each. These were labelled as A (control group), B (vaccine), C (vaccine + vitamin) and D (vaccine + aspirin). The studied parameters in the experiment were: heterophil/lymphocyte ratio, serum biochemical analysis, adrenal! body weight ratio, pathological examination of adrenal glands as well as detection of antibody response against NDV.
At the end of the experiment, it was found that no considerable stress was induced in the birds vaccinated against NDV vaccine. It was also concluded that vitamins have a positive effect on immune response and growth rate of bird.
Availability: Items available for loan: UVAS Library [Call number: 0722,T] (1).
8.
A Study On The Pathogenesis Of Yolk Retention In Broiler Chicks
by Kashif Aziz Khan | Dr. Shakil Akhtar Khan | Dr.Asim Aslam | Dr.Shahnnaz Hamid | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2002Dissertation note: The present project was designed to identify the factors responsible for yolk retention in broiler chicks. Four different experiments were conducted to find out the effect of four factors (experimental yolk sac infection, off-feed period between hatching and housing, first feeding time in the poultry house and type of initial feed) on yolk absorption.
In Experiment-1, yolk sac infection was introduced in half of chicks while remaining acted as negative control. In Experiment-2, one third chicks were housed 24 hours after hatching, one third after 48 hours and remaining one third after 72 hours. In Experiment-3, one third chicks were offered feed immediately after housing, one third after 24 hours and remaining one third after 48 hours. Water was offered to all chicks immediately after housing. In Experiment-4, one third chicks were fed with commercial starter ration, one third with ground corn during first 24 hours followed by commercial starter ration and remaining one third were offered with 2% sugar solution in first 24 hours followed by commercial starter rations. Body weight, yolk sac weight, yolk sac/body weight ratio, estimated yolk absorption and composition of yolk was studied for each group.
Results of these experiments showed that yolk sac infection led to decrease in rate of yolk absorption. Moisture and protein percentage was increased in infected yolk but fat percentage was not affected by yolk sac infection. On the other hand, post hatch starvation due to delay in housing and delay in first feeding did not affect rate of yolk absorption. Moisture percentage was not affected while fat absorption increased and protein absorption decreased due to fasting. It was also observed that feeding ground corn in early life of chick led to slow absorption of yolk contents as compared to feeding commercial starter ration and 2% sugar solution. Yolk composition was not affected by feeding commercial starter ration, ground corn and 2% sugar solution in early life of chick.
It is concluded that experimental infection with E. coli and ground corn as initial feed leads to slow absorption of yolk sac in young broiler chicks.
Availability: Items available for loan: UVAS Library [Call number: 0726,T] (1).
9.
Molecular Detection Of Babesia Bigemina And Babesia Bovis In Carrier Cattle By Duplex Polymerase Chain Reaction
by Muhammad Suleman | Prof. Dr. Zafar Iqbal Ch | Dr.Asim Aslam | Prof. Dr Abdul Rauf Shakoori | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2006Dissertation note: Babesiosis is a highly important disease in the world, caused by the intraerythrocytic protozoan parasites of the genus Babesia. A wide range of domestic and wild animals and occasionally man are affected by this disease, which is transmitted by ticks and has a worldwide epidemiological distribution. While the major economic impact of babesiosis is on the cattle industry, infections also occurs in other domestic animals , including horses, sheep, goats, pigs and dogs.
The present study targeted the carrier cattle infected with Babesia bigemina and Babesia bovis, as they are difficult to detect because of the low numbers of parasites that occur in peripheral blood. However, diagnosis of low-level infections with the parasite is important for studying the transmission and standardizing epidemiological studies. Using the Polymerase Chain Reaction (PCR) to amplify a portion of the gene from the parasite, and tested the ability of this method to detect carrier cattle.
A study was conducted to detect the. Babesia in blood samples through PCR based techniques. A PCR assay was described which could differentiate Babesia bigemina and Babesia bovis by using specific primer in carrier cattle. Blood samples of 100 cattle were randomly analyzed with PCR assay 29 (29.0%) out of 100 blood samples were positive for babesiosis in which 18% were positive for Babesia bigemina and 11% were positive for Babesia bovis, While the Light Microscopy detected only 18 (18%) out of the same samples. The samples found positive by LM were reconfirmed during the PCR assay but no sample was found to be having both Babesia bigemina and Babesia bovis infections simultaneously.
Thus it is concluded that PCR is a reliable molecular diagnostic technique to detect low level of infections in carrier animals in a population and thus could be used as an effective screening tool for the control and eradication of disease.
Availability: Items available for loan: UVAS Library [Call number: 0929,T] (1).
10.
Diagnosis Of Surra In Equines By Indirection Fluorescent Anitobody
by Malik Ahsan Nadeem | Dr.Asim Aslam | Dr.Kamran | Prof.Dr.Zafar Iqbal Ch | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Nature of contents: ; Literary form: Publisher: 2007Dissertation note: Trypanosomiasis (‘surra) is the most widely distributed arthropod- born protozoan disease affecting the equines. This study was conduàted to check the efficacy of indirect fluorescent antibody test (IFAT) for the diagnosis of Surra. For this purpose 200 blood samples were collected from horses and donkeys from different areas of Gujranwala district. Thin blood smears were prepared on clean glass slides and blood samples were centrifuged to separate the serum. Serum was transferred into the vacutainers and transported to laboratory. The serum was separated by centrifugation and stored at -70°C. 200 thin blood smear slides were fixed with methanol and subjected to Giemsa stain for further microscopic examination. Then the 200 thin blood smear slides were fixed with acetone for further processing in indirect fluorescent antibody test (IFAT). The prevalence rate of 2% and 6% by using thin blood smear and indirect fluorescent antibody test (IFAT) was obtained respectively. The results helped us to determine accuracy of indirect fluorescent antibody test (IFAT) for diagnosis of Surra.
Availability: Items available for loan: UVAS Library [Call number: 0994,T] (1).
11.
Diagnosis Of Paratuberculosis (Johne,S Disease) In Cattle And Buffaloes Through Histopathological Techniques And Polymerase Chain Reaction
by Farhan Anwar Khan | Dr.Zafar Iqbal Ch | Dr.Asim Aslam | Prof .Dr.Khushi Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2008Dissertation note: Paratuberculosis, one of the infectious disease, is the emerging cause of poor health, low productivity and finally death due to single infectious agent among dairy and beef yielding animals (cattle and buffaloes) in the World. Mycobacterium avium subsp. paratuberculosis is the most common cause of bovine Johne's disease.
The study was conducted in Lahore to compare conventional methods and PCR for the diagnosis of paratuberculosis caused by M avium subspp. paratuberculosis in 300 cattle's and buffalo's tissue samples (150 of each specie), including terminal ileum and mesenteric lymph nodes. Conventional methods included Ziehi-Neelsen's (ZN) acid fast staining and histopathology. For M paratuberculosis insertion sequence IS 900, specific 626 bp fragment, were targeted. The sensitivity and specificity of PCR was found significant in comparison to Ziehl Neelsen staining and histopathology for the diagnosis of paratuberculosis in cattle and buffaloes.
Availability: Items available for loan: UVAS Library [Call number: 1011,T] (1).
12.
Pathology Of Naturally Infected Broilers With Mycoplasma Gallisepticum And Its Diagnosis Through Pcr
by Aamir Islam | Dr.Asim Aslam | Prof.Dr.Mansur- | Prof.Dr.Zafar Iqbal Ch | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2009Dissertation note: Two hundred broiler birds (200) showing the clinical signs of respiratory signs from fifty (50) poultry farms located in and around Lahore District were analyzed for the detection of Mycoplasma gallisepticum. The tissue samples (trachea and lungs) were subjected to PCR using for Mycoplasma gallisepticum 16S rRNA gene amplification with a set of primers (MG14-F and MG13-R). Out of 200 samples, 86 were found positive with MG. These positive samples were further analyzed for histopathological changes. Lungs showed hemorrhages, congestion and massive necrosis. Lymphocytic infiltration and oedema was also observed in lungs sections. Liver showed coagulative necrosis around the central vein, congestion and infiltration of lymphocytes. Similarly, heart section revealed necrosis and degeneration in cardiac muscles. Trachea revealed the epithelial and mucosal infiltration with lymphocytes. Hypertrophy of epithelial mucosa and catarrhal exudates recorded in trachea. Sloughing of the mucosa and sub mucosa of varying degree was noted in trachea. Few birds showed no obvious changes in the organs but were positive on PCR analysis.
Availability: Items available for loan: UVAS Library [Call number: 1055,T] (1).
13.
Comparison Of Different Diagnostic Techniques For John'S Disease In Small Ruminants
by Saba Badar | Prof.Dr.Zafar Iqbal Ch | Dr. Mansur-ud-Din | Dr.Asim Aslam | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2010Dissertation note: Paratuberculosis is one of the most hazardous infectious diseases, causing heavy economic losses due to poor health, low productivity and high fatality rate among domestic and wild ruminants. Mycobacterium avium subsp. paratuberculosis is the etiological agent of Bovine Johne's disease. In this study PCR were used to detect the presence of the Acid Fast Bacillus Mycobacterium paratuberculosis, in the intestinal tissues and Mesenteric Lymph Nodes of small ruminants causing Paratuberculosis. PCR was compared to HEY medium culture on the Herrold's Egg Yolk Media.
The samples were collected from Lahore Slaughter house and brought to the Molecular Pathology Laboratory at the Department of Pathology, University of Veterinary and Animal Sciences, Lahore. The study was conducted to compare PCR and the HEY medium culture for the diagnosis of paratuberculosis caused by M avium subsp. Paratuberculosis. A total of 500 tissue samples, 250 of the ileum and 250 of the mesenteric lymph nodes were collected randomly for the identification of Johne's disease. All samples were inoculated on the HEY medium prepared in the same laboratory aseptically. Followed by DNA extraction through the Kit method then run the PCR for insertion sequence IS 900, specific 626 bp fragment, were targetted in the genome of M paratuberculosis.
The results of the study showed more samples detected positive by PCR as compared to conventional culture methodology. Also they showed in the mass of 500 tissue samples that more bacilli are prone to the samples of small intestines than associated mesenteric lymph nodes. Regarding the sensitivity of the two techniques the PCR seemed more sensitive to detect the mycobacterium in the tissues than the conventional, laborious and time consuming HEY medium culture technique; though culture has been used as golden standard in this study also. When statistically analyzed results were insignificant due to small sample size.
The study will help in comparison of the two latest techniques for the diagnosis of M paratuberculosis, to check the validity of the better technique. In this study the sensitivity and specificity of PCR was checked and compared with culture on the HEY medium staining for the diagnosis of paratuberculosis in small ruminants.
Availability: Items available for loan: UVAS Library [Call number: 1077,T] (1).
14.
Immunohistochemical And Pathomorphological Studies Of Chronic Granulomatous Enteritis (John'S Disease) in Bovines
by Muhammad Shahid | Prof.Dr.Zafar Iqbal Ch | Dr.Asim Aslam | Prof.Dr.Khushi Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2010Dissertation note: Paratuberculosis, a disease caused by Mycobacterium paratuberculosis is a peril for both livestock and human beings. The present project was designed to study the pathmorphological changes induced by the organism and standardize more reliable diagnostic techniques to identify the M paratuberculosis. Tissue samples from ileurn and mesenteric lymph nodes were randomly collected from 1 50 cattle and buffalo, each in present study that was conducted in Lahore.
Gross lesions were recorded on a Performa. The samples were subjected to acid fast staining of smears from pellets after density gradient centrifugation and paraffin embedded tissue sections. All the samples also subjected to polymerase chain reaction and immunohistochemistry. The smears prepared from bacterial pellets of mucosal and cortical scraping of terminal ileum and MLN were stained indicated 11.4 % small intestine and 12.7% lymph nodes of cattle's and 8.7% and 10.7% lymph nodes of buffalo's tissue samples were positive. ZN staining of paraffin embedded tissue showed 8.0 % small intestine and 10% MLN of cattle's and 6.0 % of small intestine and 8.7% MLN in buffalo's tissue samples were positive. On basis of PCR 5.4% intestinal tissue samples and 6.0% MLN of cattle were positive. 3.4% intestinal tissue samples and 07(4.7%) MLN of buffaloes were positive. In buffaloes 4.0% intestinal tissue samples and 6.0% MLN were positive by IHC. In cattle 6.7% intestinal tissue samples and 8.0% MLN tissue samples were positive by IHC. In cattle, 27/150(18.0%) animals showed lesions in both intestine and mesenteric lymph nodes while 5/32 (15.7%) animals showed lesions in lymph nodes only. Out of 27/150(18.0%) intestinal tissue samples, 20/27 (74.1%) samples showed corrugation of the intestinal mucosa while 7/27 (26%) showed diffuse thickness.
In buffalo, 24/150 (16.0%) animals showed lesion in both intestine and mesenteric lymph nodes while 2/26 (7.7%) animals showed lesion in lymph nodes only. Out of 24 intestinal tissue samples, 19/24(79.2%) with gross lesion, samples showed corrugation of the intestinal mucosa while 5/24(20.9%) showed diffuse thickness.
In histopathology 20/27 samples of cattle showed focal granulomatous lesions while 7/27(26%) samples showed sever infiltration of macrophages and lymphocytes while 28/32(87.5%) lymph nodes showed infiltration of paracortical and cortical region of the lymph nodes with macrophages ,lymphocytes and multinucleated giant cells While 4/32 (12.5%) samples showed moderate infiltration of macrophages.
In buffaloes 19/24 (12.7%) samples showed focal granulomatous lesions while 5/24 (20.9%) samples showed sever infiltration of macrophages and lymphocytes while 22/26 (84.7%) lymph nodes showed infiltration of paracortical and cortical region of the lymph nodes with macrophages ,lymphocytes and multinucleated giant cells While 4/26 (15.4%) samples showed moderate infiltration of macrophages.
The sensitivity and specificity of immunohistochemical method was found significant in comparison Ziehl-Neelsen staining and histopathology for the diagnosis of paratuberculosis in cattle and buffaloes.
Availability: Items available for loan: UVAS Library [Call number: 1078,T] (1).
15.
Polymerase Chain Reaction And Restriction Fragment Length Polymorphism (Rflp) By Using Ssu-r DNA Amplification for the Species Specific Diagnosis of Trypanosomiasis in Horses
by Naveed Sabir | Prof.Dr.Zafar Iqbal Ch | Dr.Asim Aslam | Prof.Dr.Khushi Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2010Dissertation note: In the current research project, a pari-trypanosome polymerase chain reaction (PCR) was optimized by using 18S single sub unit ribosomal DNA amplification and restriction fragment length polymorphism (RFLP) was also optimized and evaluated for the species specific diagnosis of the trypanosomiasis in horses.
Blood samples from one hundred (100) suspected horses were collected aseptically from different localities of Lahore. Fresh blood smear was prepared from each sample. After drying and fixing with absolute methanol, the slides were stained with Giemsa stain. Microscopic examination of stained blood smears revealed 8 positive samples out of one hundred (100) suspected horses. Polymerase chain reaction (PCR) was carried out on the same trypanosomiasis suspected blood samples to evaluate its sensitivity. Genomic DNA was extracted by using Genomic DNA Purification Kit (Fermentas mci., USA). The PCR was performed in a 50 tl reaction mixture. The tubes containing PCR mix were subjected to amplification cycles in a thermocycier after adjusting the amplification conditions.
After completion of the amplification cycles, the PCR product was characterized by 1.2 % agarose gel electrophoresis along with 100 bp DNA ladder to estimate the size of the PCR product and the gel was photographed with a Polaroid camera. PCR gave a higher percentage of positive cases i.e. 21% as compared to microscopic examination. Semi-nested polymerase chain reaction was carried out on product of the first run amplification by using same reaction mixture and amplification conditions except for template DNA. In case of semi-nested PCR 1 tl of the simple PCR product was used. Semi-nested PCR gave 100% (21/21) results. Restriction fragment length polymorphism (RFLP) analysis was conducted on nested products of the positive samples. A reaction mixture of 20 1iJ was used and samples were incubated over night at 37 °C in an incubator. The restricted products were characterized by 2 % agarose gel electrophoresis along with 100 bp DNA ladder and photographed with Polaroid camera. Restriction fragment length polymorphism (RFLP) analysis of the nested products revealed that none of the species including T. congolense, T. theileri, T. brucei and T. vivax was found in all (2 1%) positive animals having trypanosoma infestation.
It can be concluded from current study that a pan-trypanosome polymerase chain reaction is a superior and sensitive test as compared to Giemsa stained blood smear examination. The test can not only be used for early diagnosis of the trypanosomiasis but it can also be used to screen out the carrier animals those act as a reservoir of the infection for the horses and other susceptible animals. The advantage of this test is its sensitivity, universal applicability and the existence various possibilities for restriction enzyme analysis of the amplified region depending on the trypanosome species.
Availability: Items available for loan: UVAS Library [Call number: 1079,T] (1).
16.
Tissue Residue Studies Of Enrofloxacin In Broilers Chicks
by Irfan Irshad | Prof.Dr.Zafar Iqbal Ch | Dr.Asim Aslam | Prof.Dr.Muhammad Athar Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2009Dissertation note: Poultry industry is the second largest industry of Pakistan. Antibiotics are enormously used in poultry both for prophylactic and treatment purpose. Irrational use of antibiotics in poultry industry has led to the serious concern among the general public. It has also resulted in emergence of drug resistance in many susceptible organisms. The present study has therefore been planned for quantitative detection of Enrofloxacin residues; in tissues (liver, kidney, fatty tissues and muscles) of broiler birds. So, the present study was designed to detect Enrofloxacin residues in tissues of birds reared under experimental conditions and routinely slaughtered at different poultry shops. The study was completed in two phases. In phase-I, tissue samples from 75 broiler birds reared at Department of Pathology, University of Veterinary and Animal Sciences, Lahore were analyzed for quantitative detection of Enrofloxacin by HPLC. In phase-TI, the 25 broiler birds were purchased from various poultry shops of different local markets of Lahore. Tissue sample (Liver, Kidney and thigh muscles) from these broiler birds were also analyzed for quantitative detection of Enrofloxacin by HPLC. In experimentally reared birds, the highest concentration of Enrofloxacin observed was 306ng/g. In the birds injected with Enrofloxacin intramuscularly the overall highest concentration was 68ng/g. The concentration in kidney, liver and thigh muscles was in the range of 28-64 ng/g, 26-63 ng/g, 26-68ng/g in kidney, liver and thigh muscles respectively in birds injected with drug intramuscularly. The drug residues were detected up to 120 hours post treatment in intramuscularly injected birds. In orally treated birds level of Enrofloxacin in the kidney, liver and muscles were between 56-2 17 ng/g, 29- 306 ng/g ,27- 170 ng/g. The residues were detected up to 96 hours post treatment in birds given Enrofloxacin orally. The result of phase-Il showed that among the 75 market samples, 10 (40%) muscles, 8 (32%) liver and 7 (28%) kidney samples showed the Enrofloxacin residues. Out of 25 samples in which Enrofloxacin residues were detected 20 (80%) samples showed the residues concentration above MRL.
This study helped us in drawing true picture about Enrofloxacin drug residues in poultry meat and it is clearly indicated that proper withdrawal time is not being observed while marketing birds. This poses a great health concern for end consumer.
Availability: Items available for loan: UVAS Library [Call number: 1086,T] (1).
17.
Detection Of Canine Parvo Infection At Different Pet Clinics In Lahore Through Haemagglutination (Ha)
by Asif Ali | Prof.Dr.M.Younus Rana | Dr.Asim Aslam | Prof.Dr.Kamran Ashraf.
Material type: ; Format:
print
Publisher: 2011Dissertation note: Canine parvovirus, caused by a haemagglutinating canine parvovirus (CPV), one of the most important acute viral infectious diseases of pups, had been prevalent in the country. In the present study, 200 faecal and blood samples, from clinically suspected cases of parvovirus diseases dogs were collected from five pet centers of Lahore. Serum samples were harvested for hemaglutination inhibition test while the faecal samples were diluted and centrifuged to collect the supernatant. Being a haemagglutinating (HA) virus, the pre-filtered supernatant from all suspected samples was checked for any HA activity using 1% washed chicken erythrocytes. Out of total of 200 samples, 127 samples were found HA positive. Postmortem of dead dogs suspected for CPV infection was done and various gross pathological lesions were noted. Blood filled intestine lumen was found during postmortem. When lumen of intestine was opened it gave washed out appearance of intestine as mucosa was severely sloughed off. The heart of young puppies showed marbled appearance due to the presence of whitish layer on heart. Tissue samples duodenum, jejunum, ileum and heart were preserved in 10% formalin solution. The CPV cases were also observed in the locally found cross breed. This could be due to the emergence of the new stains of parvo virus due to which the cross breed getting affected.
Availability: Items available for loan: UVAS Library [Call number: 1260,T] (1).
18.
Effect Of Vitamin-E Supplementation On Lead Toxicity In Japanese Quail (Coturnix Japinica)
by Yaseen Humayun | Dr.Asim Aslam | Dr.Kamran | Dr.Muhammad Younas Rana.
Material type: ; Format:
print
Publisher: 2011Dissertation note: Quails are farmed in large number in Pakistan. Due to improper manage mental rearing system for quails in Pakistan the chances of lead toxicity is more in country through feed and fences and water. A total number of 420, day old chicks of Japanese quail were procured from the hatchery of Avian Research and Training Centre (ARTC), UVAS, Lahore. They were assigned seven dietary treatments. There were three replicates in each treatment group and each replicate was of twenty chicks. Control diet group A received only basal diet without any supplementation. Group B received basal diet + 50 mg/kg Pb. Group C received basal diet + 75 mg/kg Pb. Group D received basal diet +100 mg/kg Pb. Group E received basal diet+50 mg/kg Pb+40 mg/kg vit-E. Group F received basal diet+75 mg/kg Pb + 40mg/kg vit-E. Group G received basal diet+100 mg/kg Pb + 40mg/kg vit-E. The body weight of each the birds were carried out weekly and significant results were observed. Blood samples were collected on the 21st (3rd week), 28th (4th week), 35th (5th week) and 42nd (6th week) days of dietary treatment from two birds from each group to evaluate the liver, kidney functions and non significant results were observed. In liver mild degeneration of hepatocytes and increase in Kupffer cells while degeneration in the epithelium and mild fibrosis in interstitial tissue and cystic dilatations in the tubules and hyaline casts in the lumens of kidney tubules were major pathological lesions caused by lead and vitamin-E were observed.
Availability: Items available for loan: UVAS Library [Call number: 1264,T] (1).
19.
Remodeling Of Histopathological Changes And Immunostimulatory Effect Of Probiotic Vitamin E-Selenium And Aniseed Supplementation in IBD Vaccinated Broiler Birds
by Aima Idrees | Dr.Asim Aslam | Prof.Dr.Azhar | Prof.Dr.M.Younus Rana.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2011Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1275,T] (1).
20.
Effect Of Mentofin On Serum Biochemistry And Antibody Response Of Broiler To Avian Influenza H9N2
by Rizwana sultan | Prof. Dr.Asim Aslam | Dr Gulbeena Saleeem | Dr. hafsa zaneb.
Material type: ; Format:
print
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1907,T] (1).
21.
Standardization Of Multiplex Rt-Pcr For The Diagnosis Of Avian Influenza (H9) Virus Newcastle Disease Virus (Ndv)
by Asad Shahzad | Dr.Asim Aslam | DR. Jawad Nazir | DR.Raheela Akhtar.
Material type: ; Format:
print
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1917,T] (1).
