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1. Effect Of Diets Formulated With Different Nutrient Densities On Performance And Blood Parameters Of Broilers At Different Phases.

by Hafiz Gulfraz Ahmad (2007-VA-089) | Prof. Dr. TalatNaseer Pasha | Prof. Dr. Saima | Prof. Dr. Athar Mahmud.

Material type: book Book; Literary form: not fiction Publisher: 2015Dissertation note: Poultry sector, the second largest industry of Pakistan, is growing exponentially to fulfill the growing needs of meat and eggs. Although poultry sector has shown incredible growth rate during the past decade; however, there is still gap between poultry meat demand and supply. Every living animal has a need for protein. It is the basic structural material from which all body tissues are formed. Dietary protein and metabolize-able energy content has re-emerged as a topic of interest and importance over the last few years mainly due to environmental concerns. Availability of balanced and economical ration has great importance in broiler production. Feed cost is the single most important factor, which accounts more than 70 percent of total cost in poultry production. The challenge for nutritionist and production managers is not only to provide a feed cheaper to a maximum possible level, but also to maximize profitability, because performance of the bird depends upon a broad range of dietary nutrients. Two hundred day old chicks were purchased from the commercial hatchery. On the first day all chicks were weighed and were separated into 4 dietary treatment groups in completely randomized design so that each experimental treatment consist of 5 replicates having 10 chicks in each replicates in this way each treatment consist of total 50 chicks(4x5x10=200). Feeder and waterer were clean before start of experiment and were cleaned regularly till the end of experiment. Feed and water which were provided throughout experiment were clean, Ad libitum and completely hygenized. Four experimental diets were prepared according to the Aviagen Recommendation (2012) but the level of ME in all groups were different, HD feed with ME (3000-3200 Kcal/Kg) and CP (19-24 %), MD feed with ME (2900-3100 Kcal/Kg) and CP (19-23), ND feed with ME (2800- 3000 Kcal/Kg) and CP (19-23 %) and LD feed with ME (2700-2900 Kcal/Kg) and CP (18-23 %) respectively. Data were collected for different parameters of growth performance, carcass traits and blood TG and HDL in mg/dl, respectively. The economics of each treatment was analyzed at the end data was analyzed through Analysis of Variance (ANOVA) with use of Completely Randomized Design. The highest weight gain (1862.4± 106.56) was observed in group HD having (ME: 3000 Kcal/Kg in Starter ration, 3100 Kcal/Kg in grower ration and 3200 Kcal/Kg in finisher ration) and lowest (2974.6±26.96) was observed in group LD having (ME: 2700 Kcal/Kg in Starter ration, 2800 Kcal/Kg in grower ration and 2900 Kcal/Kg in finisher ration) at 35 days of age. Overall result showed that there was significantly decline in weight gain with decreasing level of ME in diet .Statistical analysis showed that all treatments in starter and finisher rations were different (P≤0.05) except in finisher ration. Carcass traits were non-significant (p > 0.05) to each other throughout experiment. Dressing percentage of broilers of different treatment groups was highest (60.38±7.90) in MD group and lowest was observed in LD feed group (54.6±10.37). Dressing percentage was increased as the level of metabolize-able energy increased in diets. The highest blood Triglyceride level (36.3±2.5) was observed in HD group and lowest (29.5±3.92) was observed in ND group. Statistical analysis showed that there was a strong significant difference (P≤0.05) among all these treatment. Same results were found in case of HDL levels in blood. Data analysis proved that MD feed was most economical with good results. Availability: Items available for loan: UVAS Library [Call number: 2308-T] (1).

2. Effect Of Different Bedding Materials On The Growth Performance, Carcass Characteristics And Immune Response Of Three Different Broiler Strains

by Muhammad Irfan (2013-VA-966) | Prof. Dr. Athar Mahmud | Dr. Shahid Mehmood | Prof. Dr. Saima.

Material type: book Book; Literary form: not fiction Publisher: 2015Dissertation note: Broiler birds spent most of their life in close association with bedding material. Managing poultry house bedding material is an important husbandry practice. The type of bedding material can affect the performance of the broilers to a certain extent in terms of growth performance, carcass characteristics and immune response. In Pakistan, selection of bedding material is dependent upon season and availability with little knowledge of interaction with particular broiler strain. Although, it is well documented that different strains have the tendency to perform differently in similar environmental conditions still performance of particular cross on a given bedding material is not studied. Poultry industry is constantly interested in evaluating the performance of the commercially available broiler strains. The concept of using different bedding materials for three different commercial broiler strains was evaluated at the Poultry Research and Training Centre (PRTC), Ravi Campus Pattoki, University of Veterinary and Animal Sciences, Lahore. A total of 360 day old commercial broiler chicks from three different strains (Ross 308, Cobb 500 and Arbor acres) 120 from each strain were purchased from the local market. All the day old chicks having uniform body weight were randomly distributed into 36 replicates having 10 chicks each and were fed the same quality of feed. Birds from each strain were reared on each bedding material in three replicates to evaluate the ultimate growth performance, carcass characteristics and immune response influenced by four different bedding materials for the period of 5 weeks. Three birds were randomly picked up from each treatment group at the end of trial and 5 ml blood was collected in marked test tubes from Jugular vein of each bird during slaughtering to obtain serum samples. The data were analyzed through analysis of variance (ANOVA) technique using PROC GLM in SAS 9.1. Means were compared using Duncan’s Multiple Range (DMR) test. The findings of the present study exhibited that Cobb 500 reared on corn cob pulp showed significantly (P<0.05) better final weight gain, feed intake, feed conversion ratio,European Production Efficiency Factor (EPEF), Point Spread (PS) and Livability. Regarding carcass characteristics better dressing proportion, breast yield, leg quarter yield, giblet weight and other body measurements were observed in Cobb 500 followed by Ross 308 and Arbor acres strain reared on other bedding materials used in this experiment. Corn cob pulp showed maximum potential with the best moisture absorbing capacity and favored the growth of birds followed by rice husk, wheat straw and wood shavings. Wood shaving proved to be a poor choice as it hinders the growth performance and was hard to manage due to wet, scaly and cake formation that promoted pathogenic growth leading to FPD. Moreover, corn cob pulp was better suited to promote dry condition because of fine particles size allowing more surface area for moisture evaporation and keeping it dry for longer duration. Therefore, birds kept on corn cob pulp were more comfortable as it is evident from the results that it has the potential to be used in poultry industry as alternate bedding material. Rice husk performed next to corn cob pulp while wheat straw and wood shavings did not impress by the outcome and proved to unfit for better growth and welfare of birds. In the current study, birds generated protective antibody titers against Newcastle Disease virus and no significant differences were found by using different bedding materials or broiler strains.   CONCLUSIONS AND RECOMMENDATIONS Based on the observations made from the current experiment it may be claimed that: • Corn cob pulp proved to be the most efficient as it significantly improved the growth performance and carcass characteristics of 3 commercial broiler strains especially for Cobb 500. • Corn cob pulp being most comfortable promoted health and welfare of birds leading to improved immunity against pathogens and viral diseases. • FPD can be reduced to minimum by adopting corn cob pulp as bedding material due to its fine particles and highly moisture absorbing abilities. • The highest carcass yield, dressing proportion, organ weight (heart weight, abdominal fat, liver, spleen, heart, thymus, bursa) along with breast yield, neck, keel and shank length were recorded by Cobb 500 on corn cob pulp. • Cobb 500 strain was found better than rest of two strains (Ross and Arbor acres) regarding growth performance and carcass characteristics. • Being a by-product of corn processing, corn cob pulp is the cheapest and can be an economical alternate to conventional bedding materials for all strains especially for Cobb. In Pakistan rice husk and wood shavings are abundantly used bedding materials depending upon season and availability. Rice husk is by product of rice processing and highly dependent upon season, while poultry farming is a continuous business so its availability stands as a major issue. Whereas, corn cob pulp being a by-product of corn processing is usually left on fields or wasted. So it can be used as a cheap alternative with better performance but its availability around the year and on commercial scale can be an issue. Availability: Items available for loan: UVAS Library [Call number: 2424-T] (1).

3. Physiological, Behavioral And Immune Response In Three Commercial Broiler Strains Under Four Heating Systems

by Muhammad Jamil Alvi (2008-VA-480) | Prof. Dr. Athar Mahmud | Prof. Dr. Muhammad Akram | Prof. Dr. Saima | Dr. Shahid Mehmood.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: The performance of broilers maintained under four different brooding systems with respect to behavior of the birds, blood profile and immune response in comparison to different strain of broilers raising needs to be studied under local conditions as it has been reported to possess better prospects. Furthermore, broiler performance in four different brooding zones having different air quality needs to be investigated in view of their effect on performance of broilers. In the trial, 360 commercial (Hubbard, Ross, Cobb) day-old broiler chicks purchased from the local hatchery. Hence the present study was conducted, Department of Poultry Production, University of Veterinary and Animal Sciences, Ravi campus, Pattoki with the objectives to compare the effect of strains and heating sources on the behavior (Walking, Feeding, Jumping, Litter Pecking, Lying, Standing, Preening, Breathing, Fearfulness, Body stretching, Rubbing beak with body) of the birds. In the present study effect of strains and heating system on blood biochemistry and immune response were also identified. The behavior of broilers strains studied under four different heating systems. Each treatment replicated 03 subgroups. The experimental chicks randomly divided into 36 experimental units of 10 chicks each after the adaptation period of three weeks. The heating system will be split into 04 groups (A, B, C and D). The birds in group A kept over hot water pipe lines. The birds in group B will be kept in gas heater area. The birds in group C kept in diesel brooder area. The birds in group D kept under pen cake brooder area. The data were analyzed according to analysis of variance (ANOVA) technique in Completely Randomized Design (CRD) under factorial arrangements using GLM procedures. Means separated through Duncan Multiple Range (DMR) test with the help of SAS, 9.1. Results of the present study showed significant highest jumping behavior in birds reared on hot water pipes as compared to gas heater during starter SUMMARY 48 phase and grower phase. In interaction, significantly more jumping behavior was recorded on electric bulbs in Ross when compared with Ross on hot water in starter phase. Significantly highest aggression was showed in birds reared on gas heaters than birds reared on electric bulb and diesel brooder. The preening behavior significantly improved in birds reared on gas heaters in starter, grower and finisher phase. While minimum was recorded on hot water during starter, grower and finisher phase. The feather picking behavior and walking behavior significantly higher in birds reared on gas heater during starter, grower and finisher phase. The laying behavior was statistically significant in Cobb broiler chicks during finisher phase. The feeding and litter picking behavior significantly higher in Ross broiler chicks during finisher phase. The under heat source, stretching and rubbing beak with body behavior significantly improved in Hubbard broiler chicks during starter phase. The blood profile was not significantly influenced by strains and heating source. The antibody titer against ND and IBD was higher in Ross reared on water heating system as compared to other strains and heating sources while blood biochemical profile was also higher in Ross when reared at floor heating system. Conclusion:  Ross reared under hot water during brooding showed better and healthy behavior including jumping, aggression, walking, standing, drinking, lying, feeding and litter picking  Ross strains exhibited better immune response and serum chemistry at hot water brooding system. Suggestion and Recommendations:  Hot water can be used as alternative brooding source  Behavior of commercial poultry needs further investigation and research. Availability: Items available for loan: UVAS Library [Call number: 2428-T] (1).

4. Bio-Conversion of Molasses to Phytase Through Solid State Fermentation With Aspergillus Niger

by Faseeha Nasim (2012-VA-633) | Dr. Abu Saeed Hashmi | Ms. Faiza Masood | Prof. Dr. Saima.

Material type: book Book; Literary form: not fiction Publisher: 2015Dissertation note: CD Corrupt. Availability: Items available for loan: UVAS Library [Call number: 2484-T] (1).

5. Impact Of Different Protein Sources With Digestible Lysine, Methionine + Cysteine Ratios On Production Efficiency, Carcass Characteristics, Gut Development And Total Tract Protein Digestibility In Broilers

by Ghulam Mustafa (2009-VA-471) | Prof. Dr. Anujum Khalique | Prof. Dr. Saima | Prof. Dr. Athar Mehmud.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: Due to price volatility of highly digestible but expensive protein sources like SBM future poultry diets will be formulated by using cheap and less digestible protein sources including CM and SFM due to price volatility. Undigested protein in hind gut subjected to microbial fermentation, resulted into formation of nitrogenous products, which are not only harmful to birds, but also excreted in litter bedding and generate ammonia, nitrous acid and nitrous oxide, the latter is powerful greenhouse gas with an effect about 300 times that of carbon dioxide. The potential solution to this problem is supplementation of synthetic amino acids (nearly 99 % digestible) in low nutrient density (crude protein) formulated by using cheap and less digestible protein sources. Therefore, objectives of current experiment were to determine the impact of digestible Lys, Methionine + Cysteine (M + C) ratios from different protein sources on production efficiency, carcass characteristics, gut development and total tract digestibility of protein in broilers. In total, mixed sex Ross 308 day old broilers were randomly assigned to 9 dietary treatments with replication of 6 replicates per treatment and 8 birds in each replicate. 1) Control corn soy based diet, 2) Canola meal (CM) based high crude protein ( 21% during starter phase and 19% during grower phase) diet with ideal protein ratio (IPR), 3) CM based high protein diet with 0.05 supplemented ( M + C ), 4) CM based low protein (19% during starter phase and 17% during grower phase) diet with IPR, 5) CM based low protein diet with 0.05 supplemented ( M + C ), 6) Sunflower meal (SFM) based high protein diet with IPR, 7) SFM based high protein diet with 0.05% supplemented (M + C), 8) SFM based low protein diet with IPR and 9) SFM based low protein diet with 0.05 % supplemented (M + C). All diets were fed as mash form in two phases (Starter 1-21 days and Grower 22-42 days). Feed and water were provided ad libitum throughout the experimental period. Celite @ 2% were used an inert marker for digestibility CHAPTER 6 SUMMARY SUMMARY 42 study. Average body weights, FCR, mortality rate were recorded from each replicate for the estimation of production efficiency factor. At 42 day, fresh excreta were collected from each replicate and 2 birds from each replicate were randomly selected and slaughtered for carcass and gut measurements. Crude protein and acid insoluble ash (AIA) in feed and excreta samples were determined. The results of the present study indicate that the protein sources showed non-significant difference on production efficiency and gut development, however, significant difference was observed on carcass characteristics and total tract digestibility. The birds fed higher CP level showed significant difference on production efficiency, carcass characteristics and total tract digestibility of protein. Amino acid supplementation showed significant difference on production efficiency, carcass characteristics, gut development and total tract digestibility of protein. In this study, CM and SFM showed poor performance regarding production efficiency, protein digestibility, gut development and carcass characteristics compared with SBM. However, the supplementation of Meth had improved the performance. It can be concluded that Meth supplementation may improve the overall performance in broilers fed poorly digestible protein source (CM and SFM). Further research is needed to evaluate the different strategies to improve the digestibility of poorly digestible protein sources including guar meal. Threonine also has an important role in gut development, so there is a need to study the effects of supplementation of threonine above the recommended levels in broilers fed poorly digestible protein sources. There is a need to evaluate the other protein sources that may replace SBM in broilers. Availability: Items available for loan: UVAS Library [Call number: 2586-T] (1).

6. Protease Supplementation Influences Growth Performance, Gut Health And Ileal Digestibility Of Protein In Broilers Fed Low Protein Diets

by Shahzad Ahmad (2008-VA-240) | Prof. Dr. Talat Naseer Pasha | Prof. Dr. Saima | Prof. Dr. Athar Mahmud.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: Proteases are protein-digesting enzymes that are used to break down the proteins of various plant materials and proteinaceous anti-nutrients in plant protein feedstuffs. The use of exogenous proteases can help in the reduction of the protein contents of a given feed by improving the digestibility and availability of the protein thereby reducing the cost of feed contributed by the protein feedstuff. It will also help to reduce the nitrogen excretion by the poultry and will contribute to mitigate the climate change. In the present study the protease from a bacillus lachniformis spp was supplemented to evaluate the effect of enzyme on protein digestibility, gut health and birds performance. For this three experimental diets with 21, 19 and 17 % CP (HP, MP and LP) with six treatments with and without exogenous protease were used in this trial to evaluate the effect of protease. Each treatment was replicated to 6 pens and 10 birds were allocated to each pen. Parameters studied regarding birds performance were feed intake, weight gain and FCR. All the growth performance parameters were recorded on weekly basis while the Ileal digestibility and gut health parameters were recorded at the 35th day of the trial. Data was analysed through completely randomized design (CRD) under factorial arrangement using ANOVA technique (Steel et al. 1997). Means were separated through Duncan’s Multiple Range test (Duncon’s, 1955) with the help of SAS 9.1. The results of feed intake, weight gain and feed intake: gain on weekly basis showed inconsistent results, however the overall performance of the birds fed low protein diets supplemented with protease showed significant results. The gut health parameters showed the significant increase in villus height. The apparent Ileal digestibility of protein was significantly increased in the MP supplemented with protease enzyme. 36 CONCLUSION Based upon the findings of this study it can be concluded that;  Supplementation of protease improves growth performance of the broilers however the results on weekly basis were inconsistent but overall growth performance data shows significant results.  Protease supplementation enhances the CP digestibility in 19 % diets compared to 21 % and 17 % CP diets.  The supplementation of protease enzyme improves the villus height and crypt depth: villus height in broilers. SUGGESTIONS AND RECOMMENDATIONS  Future researchers can further evaluate the subsequent effects of the protease on growth performance and immune response in broilers.  In future the research can be conducted to determine the effect of protease on energy digestibility and immune response in broilers. Availability: Items available for loan: UVAS Library [Call number: 2601-T] (1).



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