Seroprevalence of brucellosis in 541 cattle, 708 sheep, 780 goats and 63 human beings of one farm and 189 cattle, 125 buffaloes, 68 goats and 51 human beings of the other farm was studied. The various serologic tests used for this investigation included the slide agglutination test for initial screening, and the standard tube agglutination test (SAT) and the enzyme-linked immunosorbent assay (ELIS1) for further processing of the sera i.e. quantitation of Brucella antibodies. The higher prevalence of the disease was observed in cattle than buffaloes while goats outnumbered sheep in this respect. The prevalence of the disease in human beings was found to be related positively with the prevalence of the disease in animals.

The overall prevalence of the disease in sheep of one farm was found to be 35(4.947.), 27(3.817.) and 29(4.097.), respectively, by the slide agglutination, standard tube agglutination test (SPIT) and ELISA. Goats of one farm displayed a prevalence of 202(25.897.), 173(22.187.) and 183(23.467.) and that of the other, 3(4.417.), 2(2.947.) and 2(2.947.), respectively, by the slide agglutination, SPT and ELISA. This remarkable difference in the incidence of the disease in two farms may be attributed to the difference of

sample size.
A prevalence of 127(23.48%), 87 (16.08%) and 91(16.82%) was recorded in cattle of one farm while 30(15.877.), 19(10.057.) and 20(10.587.) cattle of the other proved positive respectively to the slide agglutination, SpiT and ELIS. In buffaloes, a prevalence of 17(13.67.) and 11(8.87.) and 11(8.97.) was noted by the slide agglutination, ST and ELISA tests, respectively. While interpreting the age-group relationship of the disease, it was found that adult and old animals had a higher prevalence than the young animals. Owing to the small number of male serum samples, the sex- based analysis of the disease could not have been adequately discussed

The enzyme linked immunosorbent assay was found to be a sensitive approach in detecting anti-Brucella antibodies than the slide agglutination and standard tube agglutination tests. The ELISA titres were, on average, about 8 times higher than the corresponding SAT titres

The results of this study have revealed an alarming prevalence of brucellosis in animals of farms which calls for an emergent response of experts for reappraisal and reassessment of the present brucellosis control situation, especially when the disease is an important zoonosis and a potential threat to the human health.