1.
Optimization Of Loop Mediated Isothermal Amplification Lamp Technique For The Molecular Diagnosis Of Surra In Domestic Animals
by Muhammad Saleem iqbal | Dr. Haroon akbar | Dr. Muhammad lateef | Prof. Dr. Asim.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2039,T] (1).
2.
Phylogenetic Analysis Of Plasmodium Species In Sparrows And Domestic Chicken
by Ghanwa ahmad | Dr. Haroon Akbar | Dr. Muhammad lateef | Prof Dr. Aftab.
Material type: ; Format:
print
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2075,T] (1).
3.
Molecular Diagnosis Of Feline Babesiosis
by Muhammad Younus Khan | Dr. Haroon Akbar | Dr. Muhammad | Dr. Muhammad Lateef.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2168,T] (1).
4.
Characterization Of Recombinant Surface Antigen-1 (Rsag-1) Of Toxoplasma Gondii Through Western Blotting Using Mouse Anti-Serum
by Mati Ullah Khan (2008-VA-107) | Dr. Haroon Akbar | Prof. Dr. Khalid Saeed | Dr. Uzma Farid Durrani.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: Toxoplasmosis is caused by Toxoplasma gondii, which is an obligate intracellular apicomplexan protozoan parasite. The infection is highly prevalent worldwide, and is important both from veterinary and human health concern. In the past recombinant surface antigen-1(rSAG-1) has been shown to be a good candidate for the development of immuno-diagnostic kits as well as Vaccine. Through immuno-blotting, Surface antigen-1 has been identified by sera collected from Toxoplasma gondii infected cats, dogs and humans. Looking at the importance of this health threatening issue, the current study was designed to characterize rSAG-1 through western blotting for development of local diagnostic kit through western blotting using mouse anti-serum. The rSAG-1 was previously expressed under the project of Grand Challenges Canada at Molecular Parasitology laboratory, Department of Parasitology, University of Veterinary and Animal Sciences, Lahore. Recombinant SAG-1 was quantified by using commercial kit based on BCA assay. The 15 μg of rSAG-1 was inoculated subcutaneously (S/C) 3 times with each 2 weeks interval in mice to raise hyper-immune serum. Blood was collected from mice two weeks after the each injection through lateral Retro-Orbital Bleeding (Sharma et al. 2014). Serum was collected by centrifugation. The rSAG-1 was electrophoresed on 12% polyacrylamide gel through SDS-PAGE technique and the protein was transferred to nitrocellulose membrane for western blotting. Anti-serum raised against rSAG-1 was cross-reacted with the rSAG-1 already immobilized on the nitrocellulose membrane. Anti-mice immunoglobulin G conjugated with Alkaline Phosphatase (AP) was used as secondary antibodies for the development of immuno-blot. Immuno-blot revealed a band of 35 KDa. Availability: Items available for loan: UVAS Library [Call number: 2397-T] (1).
5.
Exploring Anthelmintic Resistance In Ovine Haemonchosis Through Faecal Egg Dna At Livestock Research And Development Station, Paharpur, D .I. Khan
by Ghulam Hassan (2007-VA-144) | Dr. Haroon Akbar | Dr. Muhammad Imran Rashid | Dr. Muhammad Ijaz.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Gastrointestinal nematodes are recognized as a major constraint of small ruminant production system at small and large-scale farming in developing countries, leading to significant economic losses. The most important of these is Haemonchus contortus. Anthelmintic resistance now poses problems to sheep farmers throughout the world. This study has been designed to check anthelmintic resistance against haemonchosis of sheep by an in vivo method. The current study was carried out at Parasitology laboratory (Toxovacc lab), Department of Parasitology, University of Veterinary and Animal Sciences, Lahore. 100 faecal samples were collected from sheep at Livestock Research and Development Station, Paharpur, D.I. Khan. Animals were drenched with anthelmintic (Albashell containing Albendazole 2.5%, administered @10 mg/kg of body weight) orally after 1st sampling, at 0 day. The faecal samples were examined microscopically, micrometery was exploited and EPG analysis was performed by using McMaster technique. After 14 days, the second sampling was done. The fecal samples were brought and stored at 4°C in Parasitology laboratory (Toxovacc lab). Pre-trial & Post trial EPG were compared and positive samples were taken (tag#1057 Damani sheep male, tag#13 Balkhi sheep male, tag#1096 Damani female, tag#06 Balkhi female, tag#20 Balkhi) for egg isolation (Module, 2004) for egg DNA extraction through classical method of Phenol-Chloroform-Iso-Amyl Alcohol extraction. DNA samples were subjected to polymerase chain reactions (PCR) targeting β tubulin gene for detection of benzimidazole resistance at genetic level. Fecal egg count reduction percentage of 74.57% at day 14 post treatment clearly shows the presence of benzimidazole drug resistance in parasites infecting Balkhi and Damani sheep at Livestock Research and Development Station, Paharpur, D.I. Khan.
Summary
64
In conclusion, PCR-Sequencing technique finds its value in the detection of benzimedazole resistance at molecular level in eggs of Haemonchus contortus of sheep and this technique also helps the understanding of the development of drug resistance in the parasite. Availability: Items available for loan: UVAS Library [Call number: 2513-T] (1).
6.
Anthelmintic Activity Of Ginger Against Gastrointestinal Nematodes In Goats
by Muhammad Shahid (2008-VA-127) | Dr. Haroon Akbar | Prof. Dr. Kamran Ashraf | Dr. Muhammad Avais.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Gastrointestinal tract nematodes are responsible for wide range of health problems,
economic losses in goats and are characterized by impaired milk production, meat process,
decreased fertility, low kidding rates, decreased working efficiency and even death of the goats.
Gastrointestinal tract nematodes cause economic losses via morbidity and negative effects on
feed intake, nutrient utilization efficacy and also reduce young animal’s growth rate as a result,
leading to decreased productivity and performance of the infected animal. Due to such economic
losses, the control of the helminths is unavoidable which is also possible by herbal products such
as Ginger. Ginger has pharmacological and gastrointestinal prokinetic activities to cure
constipation, indigestion, vomiting, infectious diseases and helminthiasis. In the current study,
anthelmintic activity of Ginger has been tested against gastrointestinal nematodes of goats.
For therapeutic trials, a total of 75 goats positive for nematodes having EPG >150 were
selected randomly and divided into three groups named as Group A, B and C, each group
comprising of 25 animals. The goats of Group A were orally treated with crude powder of ginger
(Zingiber officinale) @ 3 gram/kg body weight, orally. Goats in group B served as positive
control (infected and treated with Oxfendazole). Group C comprised of positive animals which
were not treated during whole the experiment. The fecal samples were collected at day 14 (Posttreatment).
Drug’s efficacy was assessed on the Fecal Egg Count Reduction Test (FECRT) and
was calculated by following formula (Traversa et al. 2007).
[((Pre-treatment EPG – Post-treatment EPG)) / Pre-treatment EPG] × 100
SUMMARY
34
Data regarding therapeutic trails were analyzed by repeated measures one-way ANOVA, using
SPSS version 20.0, p< 0.05 was considered as significant. Trial was analyzed under different
parameters.
Ginger has shown good anthelmintic activity against gastrointestinal nematodes in goats
as evident from 63% reduction in EPG. It is suggested hereby to conduct a dose trial for the use
of ginger against nematodes in goats by using different dose levels including at least 5 different
groups of dosages like 3gram; 3.5grams; 4grams; 4.5grams and 5grams per Kg body weight. The
current study has highlighted the anthelmintic activity of ginger (Zingiber officinale) against
gastrointestinal nematodes in goats. More trials using this herbal product in other animals will
further highlight the importance of using this commonly-available and economical herbal
product in the control of gastrointestinal nematodes in livestock. Availability: Items available for loan: UVAS Library [Call number: 2712-T] (1).
