1.
Adverse Effects Of Kanamycin In Dwarf Goats (Teddy Goats)
by Atta Ullah, M | Dr. Muhammad Sabir | Dr. Haji Ahmad | Dr. M. Arshad Qureshi.
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Publisher: 1995Dissertation note: Kanamycin, an aminoglycoside antibiotic is commonly employed as a therapeutic agent for the treatment of many infectious diseases.
Goat farming is considered to be more profitable than other farm animals. Especially the
Teddy goats are abundently kept in plains and mountaneous and barani, areas of Pakistan on account of their rapid growth, early maturity and higher rate of twins, triplets in this species.
An experiment was conducted to observe the influence of kanamycin sulphate on the haematologic and enzymatic activity in teddy goats.
In this experiment kanamycin sulphate was injected to the goats at the dose rate of 10 mg/kg body weight twice daily for five successive days. The blood sample were taken after one week and then once a week for four weeks of injection The sera thus collected were subjected to analytical examination.
The study indicated the significant increase in the total number of erythrocytes and leukocytes. The packed cell volume and the haemoglobin concentration did not change
significantly. The activity of alkaline phosphatase, alanine aminotransferase (OPT), aspartate aminotransferase (GOT) was also estimated'. The alkaline phosphatase activity was enhanced during the experimental period which returned to normal at the end of the experiment. The value of OPT activity increased after the first week of injection and persisted through out the experiment, althou9h it decreased in the later stages of study.
On the basis of the observations made during this study, it is suggested that kanamycin do have damaging effect on the liver which has resulted in an increased activity of ALP, OPT and GOT.
The increase in the number of erythrocytes and leukocytes during this study might be due to the increase in haemopiosis, resulting in the production of immature blood cells.
Availability: Items available for loan: UVAS Library [Call number: 0442,T] (1).
2.
Studies Of Antibacterial Activity Of Azadirachata Indica Leaves (Neem)
by Naeem Butt, M | Dr. Muhammed Sabir | dr. Khushi muhammed | Dr. Razzaq Ali.
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Publisher: 1997Dissertation note: One hundred, day old chicks were reared in the college room. Before the introduction of chicks to the shed, the room was washed and disinfected. Azadirachta indica (Neem) leaves were used in the form of powdered, aqueous and methanolic extracts in three major groups i.e. A, B and C (containing 30 birds each) and D with 10 birds, which were kept as a control group.
The powdered, aqueous and methanolic extracts were given in the feed to each sub-group at 10,15 and 20mg/Kg body weight to A, B and C i.e., A1, A2 and A3 B1, B2,and B3 C1,C2 and C3 respectively. The N.D. vaccine was administered after 2 days of start of medication. The blood of the experimental and controlled birds were taken at the age of 28, 35 and 42 days. The medication was started at 21 day of age. The sera collected from the blood samples were tested for antibody response against N.D. vaccine. No antibacterial activity of extracts of Neem leaves against Staphylococcus aureous and Pasteurella multocida was observed. the titre of each group at one week post vaccination of ND vaccine to the birds, were determined. It was noted that the water extract of 10mg Neem leaves did not effect the antibody response of birds. The feed conversion ratio of the experimental and control birds were also determined weekly as shown in the Table No.14.
There is no apparent different in F.C.R. among the dosage level of powdered, aqueous and methanolic extracts as shown in the Figure-i. During the experimental period ii birds died. The postmortem findings showed E. coli infection.
Availability: Items available for loan: UVAS Library [Call number: 0496,T] (1).
3.
Antibacterial Activities Of An Antibiotic On Complexation With Metals
by Abid Riaz Ahmed | Dr. Muhammad Sabir | Dr. Khushi Muhammad | Mr. Razzaq Ali.
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Publisher: 1997Dissertation note: The work presented in this thesis concerns the role of metal ions through chelation in drug design. The area received much attention since the claim that the complexes of drug substances with metal ions are more active and less toxic than the parent drug.
In this work complexes of cephalexin with copper and zinc ions were prepared and characterized by microanalysis, IR and UV-vis spectrophotometry, magnetic measurement techniques, differential scanning calorimetry, and atomic absorption spectrometry. The complexes were found to be of ML2 type where M is the metal ion and L stands for the ligand. Both the complexes were monohydrate. On the basis of the analytical and spectroscopic data, the copper complex is suggested to possess an elongatedtetragonal copper (II) ions environment with d22 ground state, the complex was found to be mononuclear with respect to copper atom. The zinc complex possessed square-pyramidal geometry having coordination number 5.
The complexes were found to be much more active (copper complex possessed about four times enhanced activity, zinc complex about three times higher activity) against S. aureus. Similarly copper complex possessed 14 times higher activity and zinc complex about 10 times higher activity against E. coli.
This work presents a major advance to the pharmaceutical sciences. The complexes reported in this study may replace cephalexin in daily use due to their higher activities against the micro-organisms sensitive to cephalexin.
Availability: Items available for loan: UVAS Library [Call number: 0544,T] (1).
4.
Protective Effect Of Diltiazem Hydrochloride On Occurrence Of Alloxan Induced Diabetes In Rabbits
by Azeem, M | Dr.Muhammad Razzaq Ali | Dr.Haji Ahmad | Dr.Muhammad Sabir.
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Publisher: 1998Dissertation note: An investigation was carried out to study the protective effect of calcium channel blocker i.e. diltiazem hydrochloride on occurrence of alloxan diabetes in rabbits and also to see the protective effect of a herbal mixture of medicines, i.e. Kalonji, Kasni and Maithee on occurrence of alloxan induced diabetes in rabbits.
For this purpose, 20 rabbits were randomly divided into four groups i.e. A, B, C and D, comprising 5 rabbits in each and were provided food and water libitum.
Each of the members of group A (control) were given 150 mg/kg body weight alloxan intravenously into ear vein. These animals were given food and water libitum for two days, then they were fasted over night and blood samples were collected for estimation of glucose and total protein.
The results obtained showed significant increase in blood glucose level subsequent to the administration of alloxan. There was no change observed in total protein.
The rabbits of group B were given 20 mg/kg body weight of Diltiazem hydrochloride intraperitoneally and after 15 minutes alloxan was administered intravenously. The results obtained showed significant increase in blood glucose level and no effect on total protein.
Similarly the rabbits of group 'C' were given 40 mg/kg body weight of diltiazem hydrochloride intraperitoneally and then after 15 minutes 150 mg/kg body weight of alloxan was administered intravenously into ear vein. Blood samples showed significant decrease in blood glucose level and no effect on total protein.
While rabbits of group-D were given 2 gm/kg body weight of herbal mixture orally, and then after two hours alloxan was administered intravenously. The data showed a significant rise in blood glucose level and again no effect on total protein. This showed that the herbal mixture had no protective effect.
From this result, it is conceivable that diltiazem hydrochloride showed protective effect on occurrence of alloxan induced diabetes in rabbits and there was no effect on total protein whereas the herbal mixture did not have protective effect.
Availability: Items available for loan: UVAS Library [Call number: 0577,T] (1).
5.
Protective Effect Of Indomethacin On Alloxan Induced Diabetes In Rabbits
by Razaq Waria, M | Dr. Muhammad Sabir | Dr. Haji Ahmad | Dr. Muhammad Razzaq Ali.
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Publisher: 1998Dissertation note: An investigation was undertaken to study the protective effect of indomethacin and powdered Cuminum nigrum Linn. Seeds (Kala zeera) on alloxan induced diabetes in rabbits.
Thirty healthy rabbits were kept in animal house of College of Veterinary Sciences, Lahore. These rabbits were randomly divided into six groups A, B, C, D, E and F comprising of 5 rabbits in each and were provided food and water aci-libitum.
Group A was kept as control which was given alloxan (150mg/Kg body weight) without prior administration of indomethacin or Kala zeera. Group B rabbits were given 10 mg/Kg body weight indornethacin intraperitoneally, 15 minute prior to alloxan administraiton intravenously of 150mg/Kg body weight. Group C was given 20mg/Kg body weight of indomethacin intraperitoneally 15 minute prior to alloxan administration intravenously of 150mg/Kg body weight. Group D rabbits were given powdered Cuminum nigrurn 2gm/Kg body weight orally 2 hours prior to alloxan administraiton intravenously of 150mg/Kg body weight. Group E was given only 10mg/Kg body weight of indomethacin intrperitoneally and its effect was noted. Group F was given only 25mg i.e. 0.5m1 NaHCO3 5% intraperitoneally to see its effect.
The animals were sampled for glucose and protein estimation after zero 24, 48 and 72 hours. Before taking the samples animals were fasted overnight. Their blood glucose levels were estimated by glucorneter and total serum protein was estimated by Biuret method. Group A showed marked increase of blood glucose level and had not effect on total protein. Group B did not show protective effect on blood glucose level and no change in total serum protein level, while group C showed significantly protective effect on the blood glucose level and no change in total serum protein level. Group D showed marked increase of blood glucose level and did not show any protective effect and also no change in total serum protein level. Group E and F showed significant increase of blood glucose level but increased level remained in the normal limit and no change in total serum protein.
It is clear that indomethacin showed dose dependently protective effect on alloxan induced diabetes in rabbits but herbal medicine Cuminum nigrum has no protective effect. It is also clear that there in no change in total serum protein level even in severe diabetic condition.
Availability: Items available for loan: UVAS Library [Call number: 0578,T] (1).
6.
Synthesis And Evaluation Of Biological Properties Of Derivatives Of Cephalosporins
by Rehmat Ullah, Major | Dr.Muhammad Sabir | Dr.Haji Ahmad | Dr.Muhammad Raziq Ali.
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Publisher: 1999Dissertation note: This study was aimed at preparation and evaluation of the biological properties of cephradine. Schiff base transition metal complexes of cephradine were prepared. In the first step Schiff bases were prepared by condensation of the primary amine with a carbonyl compound. For this purpose pyridoxal HCI and salicylaldehyde were used. In the next step the prepared Schiff bases were chelated with metal salts using copper acetate and zinc acetate, here metal binds to polydenate ligands to form the ring structure, where metal is the part of the ring. In this way a three dimensional molecule was obtained which exhibited enhanced antibacterial activity in some cases and depressed activity in the others. The complexes were subjected to biological evaluation to find out the minimum lethal concentration of the new complexes and then it was compared with the parent drug. The standard organisms of S. aureus, .E.coli Pseudomonas aeruginosa and . pneumoniae were used to study the antimicrobial activity. Cephradine complexes showed a depressed activity against S. aureus, . E.coli and P . aemginosa. But the activity of CPC and CSC derivatives was found ten times enhanced against
K. pneumoniae, when compared with the present drug. The haematological study revealed that the parent drug and the new derivatives did not have any deleterious effect upon the total erythrocyte count, haemoglobin concentration, leukocytic count, differential leukocytic count i.e., lymphocytes and polyrnorphs (neutrophils). Thus the new compounds were found to be as safe as the parent drug itself.
Availability: Items available for loan: UVAS Library [Call number: 0602,T] (1).
7.
Synthesis And Evaluation Of Bioilogical Properties Of Derivatives Of Ceftriaxone Sodium
by Shahid Zaigham | Dr.Muhammad Sabir | Dr.Muhammad Razaq Ali.
Material type: ; Format:
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not fiction
Publisher: 1999Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0622,T] (1).
8.
Study Of Changes In Hematological Laboratory Profile Induced By Vinorelbine In Cancer Patients
by Taha Nazir | Prof.Dr.Saghir Ahmad Jafri | Dr.Muhammad Mehmood Khan | Prof.Dr.Muham.
Material type: ; Format:
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Publisher: 2000Dissertation note: The present study was carried to investigate the hematological alterations in the patients diagnosed as cancer and were administered vinorelbine as part of their chemotherapy at Shoukat khanum memorial cancer hospital and research centre. It was carried in the chemotherapy department, pathology laboratory, medical record section, pharmacy department, and data processing department of the hospital. For investigating there were a total of 60 adult cancer patients with non-small cell lung cancer (NSCL), metastatic breast cancer (MBC), and cancer of cervix (CC), with age between 24 to 71 years (Mean±SEM: 42.73±2.69) selected. Which were divided into two groups: Group - I for those patients who were on the treatment protocol of Vinorelbine alone (25 mg/m2/day one, weekly x 4) and group - II for those patients who were on treatment protocol of Vinorelbine base combinations Vinorelbine/Doxorubicin (Vinorelbine - 20 mg/m2/day one and day eight, Doxorubicin - 50 mg/m2/day one only) and Vinorelbine/Cisplatin (Vinorelbine - 20 mg/m2/day one and day eight, Cisplatin - 40 mg/m2/day one only). The Laboratory tests (C.B.C.) of all the patients were performed in the Pathology Laboratory of the hospital. After completion of the study, the collected Hematological values were statistically analyzed by student pair test. By the overall comparison of mean values over time, there were significant leukopenia (neutrophile, eosinophil, & monocyte), anemia and decreased hematocrit value observed in the patients on chemotherapy protocol of Vinorelbine alone and significant leukopenia (neutrophile only), anemia and decreased hematocrit value and hemoglobine conc. observed in the patients on chemotherapy protocol of Vinorelbine based combinations. By an independent comparison of mean values of two groups at every week, there were significantly higher toxicities 1 e leukopenia at week-3 (neutrohpil at week-3, eosinophil at weekI, lymphocyte at week-i &2) and thrombocytopenia at week-i observed in the patients on the treatment protocol of Vinorelbine based combinations. When the mean values observed before therapy were compared with that of at week-4 of both of the groups, there were significant decreases noted in TLC, neutrophil count, monocyte count, erythrocyte count, hemotocrit value and Hb conc in the patients on treatment protocol of Vinorelbine alone, and erythrocyte count, hemotocrit value and hemoglobine conc. in the patients on treatment protocol of Vinorelbine based combinations. It is concluded from this study that, there is insignificant difference in the overall hematological toxicities produced by both of the chemotherapy protocols. The clinical oncologist, consultant physicians and pharmacist, therefore now suggested to select the either of the of the chemotherapy protocol with respect of the therapeutic efficacy only
Availability: Items available for loan: UVAS Library [Call number: 0661,T] (1).
9.
Efficacy Of Various Chemical Agents Against Avian Influenza Virus (Type H9)
by Imran Altaf | Prof.Dr.Muhammad Ashraf | Dr. Khushi | Dr. Muhamad ovais Omer.
Material type: ; Format:
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Publisher: 2001Dissertation note: In the present project, 300 eggs were taken from Big Birds. They were cleaned and incubated for 9 days. After 9 days they were candled to separate the live and dead embryo. On the 11th day, the embryos were inoculated with the different concentrations of drugs which were to be tested. The detail of these drugs are given below:
Chlorine was taken in the concentration of 1, 2 and 5 ppm. They were divided into control and experimental groups containing drug + Normal saline and drug. + virus. The results showed that 2 ppm and 5 ppm chlorine was most effective as disinfectant, while 1 ppm was not effective.
Iodine was also tested in the concentration of 0.1, 0.5 and 1 per cent for control and experimental groups in the same fashion as in chlorine. The results showed that 1% and 0.5% solutions are highly antiviral but are toxic for living cells, while 0.1% iodine is virucidal against virus and can be used for the living cells as it was not toxic for embryo.
In case of amantadine 50 jig/mi, 500 jig/mi and 1000 jig/mi were used along with virus and Normal saline for experimental and control groups, respectively. Results show that 1000 i.g/ml concentration was toxic for cell and hence can not be used in vivo, while 50 jig/ml failed to stop the replication of virus. 500 jig/ml concentration not only stopped the viral replication but also did not kill the embryo. So 500 jig/mi of amantadine can be used in poultry, if desired.
Acyclovir is another pharmaceutical product, used in the concentration of 50 jig/mi, 200 jig/mi and 400 jig/mi in the fashion as described above. Results showed that acyclovir was effective within the range of 200 jig/ml to 400 jig/mi without any fear of damage to cell, but 50 jig/mi concentration failed to stop the viral replication and also showed high HA titer.
In herbal group, Soyabean was tested in the concentration of 1 g/l00ml, 5g/l00ml and 10 g/l00ml. When the results were collected it was noticed that neither concentration of soyabean has any antiviral effect against influenza.
Opuntia plant group which comprises of three species named:
0-Stricta, O-Dellinii and 0-Manocantha was testified for their antiviral efficacy. The concentrations used were 1 gm, 5 gms and 10 gms dissolved in 100 ml of Normal saline. Their extracts were obtained and injected in the embryo alongwith virus and Normal saline for experimental and control groups after giving them an interaction time of one hour. In the results, 10 g/l00ml and 5 g/l00ml show a good anti-viral efficacy against influenza, while 1 g/l00ml failed to show any significant effect and gave high HA titer. So in Pakistan these herbal drugs should be further investigated so that these can be used against influenza.
Availability: Items available for loan: UVAS Library [Call number: 0743,T] (1).
10.
Effect Of Diclofenac Sodium On Experimentally Induced Aseptic Arthritis In Equines
by Dr . Aqeel Javeed | Prof.Dr.Muhammad Ashraf | Dr . Muhammad | Dr . Muhammad Ovais Omer.
Material type: ; Format:
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Publisher: 2002Dissertation note: Aseptic arthritis of the knee joint was induced in 12 healthy donkeys, weighing 60 to 85kg by intra-articular injection ot O.3m1 of turpentine oil. The animals were randomly divided into 2 groups A and B of 6 animals each. Group A served as a control whereas in Group B anti-arthritic drug, diclofenac sodium (Diclocare 5%) was administered intramuscularly at the dose rate of 2.5mg/kg body weight daily for 5-6 days starting one day after the induction of arthritis. In group B, the joint circumference became normal on 6th day after induction of arthritis while in group A (control), the joint circumference took 16 to 20 days to become normal. According to this trial, diclofenac sodium provided an early return to normal stance and weight bearing in treated group B, taking longer time in control group A. No significant change in blood Haemoglobin, Total erythrocyte count, Total leukocyte count, Erythrocyte sedimentation rate and Packed cell volume was observed in control and treated animals. It is concluded that diclolènac sodium is an effective treatment for arthritis in donkeys.
Availability: Items available for loan: UVAS Library [Call number: 0780,T] (1).
11.
Therapeutic And Prophylactic Effects Of Antimicrobial Agents Against Omphalitis In Broiler Chicks
by Qamar Arif | Prof.Dr.Muhammad Ashraf | Dr. M. Ovais Omer | Dr. Shakil.
Material type: ; Format:
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Publisher: 2002Dissertation note: The Project was designed to study therapeutic and prophylactic effect of antimicrobial agents against omphalitis in the broiler chicks. Three hundred and seventy five day-old chicks were divided into two groups for therapeutic and prophylactic trails containing 200 and 175 chicks respectively. Therapeutic trail group was sub-divided into live groups A, B, C, D and E containing forty chicks in each. Chicks of group "A" served as non-infected, non-treated control. Infection was induced in group 13, C, D and E on first day. After 24 hours post infection, five chicks from each group were randomly slaughtered to observe the postmortem lesions of omphalitis. Trizine, Mycomas and Vetymoxil C medication was done in groups C, 1) and E respectively from 2nd to 5th day after the confirmation of omphalitis. Prophylactic trail group was also sub-divided into five groups A, 13, C, D ail E containing 35 chicks each. Group "A" served as non-infected, non-treated control. Chicks of group C, I) and E were first given medication of Trizine, Mycomas and Vety-moxil C respectively as a prophylactic measure for first 3 days of life. Then infection of confirmed pathogen of E. coil was given to group B, C, D and E on the 4th day of life. Therapeutic and prophylactic effect of Trizine, Mycomas and Vetymoxil C was compared based on feed consumption, mortality, clinical findings and postmortem lesions.
The results indicated that the treatment and prophylactic use of Mycomas, Trizine and Vety-moxil C were effective against omphalitis to reduce the early chick mortality. During therapeutic trials Mycomas cure rate was better than the Vety-moxil C and Trizine. In prophylactic trails Mycomas provided better protection followed by Vety-moxil C and trizine.
Therefore Mycomas may be used to reduce the early chick mortality caused by omphalitis.
Availability: Items available for loan: UVAS Library [Call number: 0792,T] (1).
12.
Bioavailability And Pharmacokingetics Of Ampicillin In Volunteeers
by Naseer Ahmad | Prof.Dr.Muhammad Ashraf | Dr. Muhammad Ovais Omer.
Material type: ; Format:
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Publisher: 2001Dissertation note: The bioavailability and pharmacokinetics of ampicillin were investigated in six healthy male volunteers after intravenous and oral administration of penbritin 500mg and relative bioavailability of ampicap 500mg after oral administration. The blood samples were collected at various time intervals following administration of single dose of 500mg to each individual volunteer. The concentrations of ampicillin in serum samples were determined by using microbiological assay. The serum concentrations of ampicilin at different time intervals were plotted on semi-logarithmic graph paper. The bioavailabilty and pharmacokinetics parameters were calculated and expressed as mean + S.D.
The peack oncerntration of 8.58+0.02 ug/ml reached in 2.30+0.002 hours after oral administration. By calculating the AUC i/v and AUC oral the bioavailability of penbritin 500mg oral was calculated and was 51.60+ 2.2.0 and that of ampicap was 50.00+10.00. The relative bioavailability (Bioequivalence ) of ampicap was determined by dividing the AUC (Ampicap) by AUC (penbritin) and it was 96.94+5.18%. Time to reach the maximum concerntration of penbritin orally was 2.30+0.002 and that of ampicap was 2.31+0.006 hours
The half-live of elimination after i/v administration of penbritin was 1.005+0.00 hours, after oral administration was 1.21+0.001 hours and that of ampicap was 1.21+0.004, respectively. The volume of distribution after pendbritin i/v, penbritin orally and ampicap orally was 11.08+0.091, 25.92+0.76 and 26.64+0.82 liters, respectively. The total body clearance of penbritin was 127.84+10.53, 246.70+104.13 and 254.46+45.32 ml/minutres, respectively after penbritin i/v, penbritin orally and ampicap.
Availability: Items available for loan: UVAS Library [Call number: 0812,T] (1).
13.
Efficacy Of A Newly Developed Herbal Diuretic Mixture In Broiler Chickens
by Mahreen Zahara | Prof.Dr.Muhammad Ashraf | Dr.Muhammad | Dr.Muhammad Ovais Omer.
Material type: ; Format:
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Publisher: 2002Dissertation note: A number of diuretic drugs are presently used in poultry under the name of "flushers". These drugs play an important role in poultry disorders especially in cases of ascties, liver disfunctions, nephritis, Urates deposition, restoring normal functions of kidneys by eliminating toxic substances and extra fluid (through urinary system) and in oedematous conditions. The present study was under taken to evaluate the efficacy of a new locally prepared herbal diuretic mixture containing Cuminum Cyminum, Robonate of Poast, Sneeze wort and Salt petre and was compared with uronex in broiler chicken.
For this purpose, one hundred a day-old boiler chicks were reared under standard conditions. At the age of 28 day they were randomly divided into groups A, B, C, D and E. The birds of group A, B and C were given herbal diuretic mixture at different dilutions 1%, 2% and 3% in drinking water. The birds of group D were given uronex in drinking water and birds of group E were given with normal water, being control group. Diuretic efficiency of this herbal mixture was evaluated by measuring total serum protein, electrolytes (sodium and potassium) creatinine and uric acid contents before and after drug administration in broiler chicks.
Non significant (P>O.05) changes were observed in the serum protein and serum creatinine levels. The values of serum sodium, potassium and uric acid were significantly (P< 0.05) decreased in treated birds after medication as compared to control birds.
From these observations it is concluded that the newly prepared herbal diuretic mixture was effective and can be successfully used for the elimination of toxic and oedematous fluids in broiler chicks.
Availability: Items available for loan: UVAS Library [Call number: 0813,T] (1).
14.
Role Of Alpha-2-Adrenocepter Agonists As Sedative And Analgesic In Fancy Birds
by Uzma Farid Durrani | Prof.Dr.Muhammad Asharaf | Dr.Muhammad | Dr.Muhammad Ovais Omer.
Material type: ; Format:
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Publisher: 2003Dissertation note: This project was designed to study the role of alpha-2-adrenocepter agonists as sedative and analgesic in fancy birds. A study was conducted on budgerigars pigeons and quails which were devided into three groups i.e. group A (budgerigar), group B (pigeon) and group C (quail), each group with fifty birds. Each group was subdivided into five subgroups i.e. X, D, K, X-K and D-K, each subgroup with 10 birds. All birds were physically examined and weighed. In group A, all birds of subgroups X, D, K, X-K, D-K were given xylazine (20mg/kg), detomidine (2.8mg/kg), ketamine (80mg/kg), xylazine+ketamine (1 Omg/kg+4Omg/kg) detomidine+ketamine (1 .4mg/kg+4Omg/kg) respectively via intramuscular route. In group B, all birds of subgroups X, D, K, X-K, D-K were given xylazine (16mg/kg), detomidine (1 .4mg/kg), ketamine (60mg/kg), xylazine+ketamine (8mg/kg+3 0mg/kg), detomidine+ketamine (0.7mg/kg + 30mg/kg) respectively via intramuscular route. In group C, all birds of subgroups X, D, K, X-K, D-K were given xylazine (18mg/kg), detomidine (2.4mg/kg), ketamine (60mg/kg), xylazine+ketamine (9mglkg+30mg/kg), detomidine+ketamine (1 .2mg/kg+3 0mg/kg) respectively via intramuscular route.
Different parameters i.e. time and nature of induction, duration, recovery, temperature, respiration, heart rate, reflexes and degree of analgesia were used for the evaluation of sedation and anaesthesia.
The results of this study indicated that in budgerigars, for non surgical procedures, xylazine (alone) can be used safely. For minor and major surgical procedures of budgerigars xylazine-ketamine combination can be used safely. In pigeons, for non surgical procedures, detomidine (alone) can be used safely; however, in case of unavailability of detomidine second choice is xylazine (alone). For minor and major surgical procedures of pigeons either xylazine-ketamine combination or detomidine-ketamine combination can be used safely. In quails, for non surgical procedures, xylazine (alone) can be used safely while for minor and major surgical procedures of quails, xylazine-ketamine combination can be used safely. Second choice for this purpose is detomidine-ketamine combination. All sedatives and anaesthetics should be used with proper thermoregulatory measures.
Availability: Items available for loan: UVAS Library [Call number: 0835,T] (1).
15.
Comparative Efficacy Of Various Antiviral Agents Avian Influenza Virus(Type H7)
by Tahir Mahmood Shaukat | Prof.Dr.Muhammad Ashraf | ghjgh | Prof.Dr.Khushi.
Material type: ; Format:
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Publisher: 2007Dissertation note: In this project 3 different concentrations of each of amantadine HC1, extracts of fresh leaves of opuntia herb, papaya and dried powdered leaves of green tea in distilled water were evaluated for their antiviral activity, by injecting into 10 days old live embryonated broiler chicken eggs through chorioallantoic sac route. For each drug embryonated eggs were treated only with it's 3 different concentrations to evaluate toxicity, whereas to check antiviral efficacy, embryonated eggs were treated with suspensions of three different concentrations of drug with 4HA titre of AIV (type H7).
In case of amantadine HC1 out of 50ug/ml, 500ug/ml and l000ug/ml concentrations only 500ug/ml was found to be an ideal concentration, as in addition to stop virus growth it also did not kill the embryos. In case of Opuntia dellinii all the 3 concentrations used were not toxic for embryos, but antiviral oeffect was observed only by 4gm and 8gm/100ml concentrations. Green tea extract was found to be effective against AIV only at 8gm/100ml concentration with no damage to chick embryos. Papaya leaves extract as a whole failed to check virus replication at all the three concentrations used in this experiment i.e. 2gm, 4gm and 8gm/100ml. All these dose levels were not lethal for chick embryos.
Availability: Items available for loan: UVAS Library [Call number: 0900,T] (1).
16.
Efficacy Of Livergen And Icterene Aginst Experimentally Induced Hepatotoxicity In Rabbits
by Ahmed Sardar Mirza | Prof.Dr.Muhammad Ashraf | Dr. Muhammad Ovais Omer | Prof.Dr.
Material type: ; Format:
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Publisher: 2004Dissertation note: The study was conducted on 35 male rabbits weighing from 1-1.5 kg. The study was carried out on the basis of elevation and decline in the levels of Alanine Transminase (ALT/SGPT), Aspartate Transminase (AST/SGOT) by inducing experimental hepatic injury by paracetamol and then treating it with hepatoprortective agents like, Livergen, Icterene and Jetepar. The rabbits were divided into the seven group i.e. A, B, C, D, E, F and G. All animals except animals of group A which is a control group were administered with hepatotoxic dose of paracetamol 2.5g/kg at day zero, while group C, D and E received hepatoprotective dose of Livergen syrup 1ml, 2ml and 3ml/kg respectively at day 2 for five consecutive days in addition to the toxic dose of paracetamol at day zero. The animals of group F received hepatoprotective dose of Icterene tablets 140mg/kg at day 2 for 5 consecutive days in addition to paracetamol toxic dose at day zero. The animals of group G received hepatoprotective dose of Jetepar syrup 1ml/kg at day 2 for five consecutive days in addition to the hepatotoxic dose of paracetamol 2.5g/kg at day zero. The blood samples were collected at day 0, 1, 2, 4, 6, 8 and 10. Concludingly, the drug Jetapar was found to be best effective. While Livergen and Icteren showed equal effective results at normal dose but significantly less than Jetepar. However, Livergen at dose 2ml/kg and 3ml/kg showed equal but almost similar results to Jetepar at last day.
Availability: Items available for loan: UVAS Library [Call number: 0915,T] (1).
17.
Antimicrobial Activity Of Mentha Longifolia And Artemisia Maritima
by Sakhawat Ali | Prof.Dr.Muhammad Ashraf | Dr. Muhammad Ovais Omer | Prof. Dr.
Material type: ; Format:
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Publisher: 2005Dissertation note: This study was undertaken to determine the use of medicinal plants as an antibacterial agent and its potential as an alternative medicine against bacterial infections. For this purpose water, methanol and chloroform extract of the Artemisia maritirna and Mentha Ion gifolia were tested against Staphylococcus aureus, Escherichia coli, Salmonella spp. and Bacillus subtilis by Disc Diffusion Method, Well Diffusion method and Micro Dilution Method. After determination of the antibacterial properties the data was analyzed statistically by using paired t-test and it was observed that methanol extract had greater antibacterial activity as compared to chloroform and water extract, whereas, chloroform extract had moderate antibacterial activity but the water extract had negligible antibacterial activity. From the four tested organisms Escherichia coli was more susceptible to methanol extract of Mentha ion gfolia. In general Staphylococcus aureus was more susceptible to both plant extracts as compared to other three tested microorganisms.
Availability: Items available for loan: UVAS Library [Call number: 0924,T] (1).
18.
Toxicity Of Flunixin Meglumine In Avian Species
by Muhammad Irfan | Prof.Dr.Muhammad Ashraf | Dr. Muhammad Ovais Omer | Prof. Dr.
Material type: ; Format:
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; Literary form:
not fiction
Publisher: 2006Dissertation note: This project was designed for the evaluation of different effects of toxic dose levels of flunixin meglumine and diclofenac sodium in broiler chickens. For this purpose one hundered and fifty broiler chicks were purchased from the market and were reared upto day 28 and then were divided into different groups (A, B, C, D E and F). Groups A, B, C and D were treated with flunixin meglumine at the dosage rate of 1.25, 2.5, 5.00 and 10.00mg/kg body weight JIM once daily respectively and group E was kept as Control. The remaining 25 birds of group F were treated with Diclofenic sodium 5.00mg/kg body weight by same route of administration and dosing interval as mentioned for flunixin meglumine. Treatment was continued for five consecutive days. Clinical signs, mortality, liver and kidney function tests were performed by estimation of Aspartate Transaminase (AST), Alanine Transaminase (ALT), Alkaline phosphatase (ALP), uric acid and Creatinine in the serum samples. Postmortem findings and histopathological changes of different tissues were also recorded. Toxicity of flunixin meglumine was evaluated through the above mentioned parameters and this toxicity was compared with that of Diclofenac sodium. Based on the mortality data and biochemical analysis it was concluded found that flunixin meglumine was toxic in avian species. Keeping in view the environmental problem (vultures crises) it is suspected that flunixin meglumine may cause some problems in avian species as diclofenac sodium.
Availability: Items available for loan: UVAS Library [Call number: 0925,T] (1).
19.
Effect Of Malathion On Serum Cholinesterase Activity And Lipid Profile In Rabbits
by Muhammad Imran | Prof.Dr.Muhammad Ashraf | Dr. Kamran | Dr. Muhammad Ovais Omer.
Material type: ; Format:
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Publisher: 2007Dissertation note: Malathion is an organophosphorous compound widely used as pesticide, mostly in spray form. The present project was designed to study the effect of Malathion on lipid profile and inhibition of cholinesterase activity in rabbits. The experimental rabbits were kept at the animal shed of Department of Pharmacology and Toxicology and were divided into five groups A, B, C, D, and E, each comprising of five animals. All the animals of each groups were shaved from their dorsal side for the application of Malathion. The animals of group A were treated with Malathion 50 mg / kg body weight, dermally. Group B was treated with 100mg of Malathion per kg B.W., dermally. Rabbits in group C were treated with Malathion at the rate of 200mg! kg B.W. Group D was treated with 400mg/kg B.W., dermally. The application of Malathion was repeated on Day 1, Day 3, Day 5 and Day 7 of the experiment. Group E was as control. Blood samples were collected from each animal separately before and after the experiment from their marginal ear veins. Serum was separated from the blood samples and was further analyzed for cholinesterase activity and lipid profile by spectro photometric method. Specified Randox kits were used for this purpose. Data collected from the experiment showed that Malathion inhibits the activity of cholinesterase enzyme. Also Malathion influences the lipid profile. In group A which was treated with Malathion 50mg/kg, the total cholesterol was increased up to 27.11%, high density lipoprotein (HDL) were increased up to 22.40%, low density lipoprotein were increased up to 175.44% and triglycerides (TGs) were decreased by 14.42%. In group B which was treated with Malathion 100mg/kg, total cholesterol was increased up to 34.46%, HDL increased up to 35.40%, LDL increased up to 183.09% while TGs were decreased by 14.02%. In group C which was treated with Malathion 200mg/kg, total cholesterol was increased up to 31.46%, HDL were increased up to
42.10%, LDL were increased up to 137.44% and TGs were decreased by 15.44%. While in group D which was treated with Malathion 400mg/kg, total cholesterol was increased up to 23.46%, HDL were increased up to 45.76%, LDL were increased up to 82.37% and TGs were decreased up to 14.95%.Activity of cholinesterase was decreased up to 82.74%, 91.59%, 89.63% and 86.64% in different groups like group A, B, C and D respectively. All the results were compared with the values of control group E. All the data obtained from these experiments was analyzed statistically.
Availability: Items available for loan: UVAS Library [Call number: 0974,T] (1).
20.
Effect Of Detomidine And Chloramphenicol On Propofol-Induced Ana Esthesia
by Salamat Ali | Prof.Dr.Muhammad Ashraf | Dr. Muhammad | Dr. Muhammad Ovais Omer.
Material type: ; Format:
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Publisher: 2007Dissertation note: In clinical practice anaesthetics are of great importance as for as surgeries are concerned whether in human beings or animals. This project was designed to evaluate the effect of Chioramphenicol and Detomidine on Propofol-induced anaesthesia in mice and dogs. The study was conducted in two separate experiments. In first experiment, sixty mice were divided into six groups; group A, group B, group C, group D, group E and group F. Each group comprised of ten mice. Group A was treated with Propofol (100 mg/kg). Chioramphenicol pretreatment (100, 150, 200mg/kg), 15 minutes prior to administration of Propofol (100 mg/kg) were used in Group B, C, D. Group F was treated with normal saline 0.3 ml only. Both the drugs were given as intraperitoneal injection. In second experiment, twelve dogs were divided into three groups i.e. group A, B and C. Each group comprised of four dogs. Group A was treated with Propofol (6 mg/kg, I/V) as a single bolus, while group B was given Detomidine hydrochloride (40 pg/kg body weight) intramuscularly as a premedication 15 mm before Propofol intravenously (6 mg/kg) while group C was treated with 3 mg/kg Propofol along with 40 pg/kg of Detornidine as premedication. After a wash out period of two weeks the dogs of group A were treated with Detomidine hydrochloride (40 jig/kg body weight) intramuscularly as a premedication 15 mm. before the Propofol intravenously (6mg/kg body weight) while, the dogs of group B were treated with Propofol 6 mg/kg body weight intravenously as a single bolus and group C was repeated with the 3 mg/kg Propofol along with 40 pg/kg of Detomidine as premedication. Anaesthetic parameters were noted. Serum level of Calcium, Chloride, Potassium and Sodium fell and were normal after recovery.
The results indicated that induction time remained same and was smooth with these premedication while duration of anaesthesia increased to threefold compare to control groups and uncomplicated recovery was recorded.
The collected results from this study conclude that this premedication gives better results and should be used in clinical practice.
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21.
In Vitro Antibacterial Effect Of Opuntia Dillenii And Zingiber Officinale Extracts
by Muhammad Ihtisham Umar | Prof.Dr.Muhammad Ashraf | Dr.Aftab Ahmad Anjum | Dr.Sheryar Afzal.
Material type: ; Format:
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; Literary form:
not fiction
Publisher: 2008Dissertation note: This study was designed to test the antibacterial activity of Opuntia dillenii (Chhittarthohar) and Zingiber officinale (Ginger) extracts in four different solvents i.e. petroleum ether, chloroform, methanol and water against Staphylococcus aureus, Bacillus subtilis, Escherichia coil and Salmonella lyphi. Plant material was cut into small pieces and dried in desiccators. Each plant material was weighed and 20.0 grams of it was taken in four different bottles and the bottles were labeled as petroleum ether extract, chloroform extract, methanol extract and water extract. 500.0 ml of each solvent was added in the respective bottle. Plant material was macerated for three days. The extracts were filtered by whatmann's filter papers, dried in vacuum desiccators and the powder mass obtained was weighed and then reconstituted in respective solvent to get the final extract of known concentrations.
Each of the bacteria was inoculated separately in the nutrient agar medium in a concentration of 106 CFU/ml and the media was poured in petri dishes and was allowed to solidify. Five wells of 1 .0 centimeter diameter were cut in each petri dish by the help of a cork borer. 200pA of plant extract (containing 2000 jig) was poured in one well and 400 jil of extract (containing 4000 jig) was poured in second well. Gentamicin (400 jig per well) and penicillin-G (640 jig per well) were used as positive controls and respective solvent was used as negative control for each extract. The plates were remained open for 20 minutes in laminar flow hood, allowing organic solvents to evaporate and then the plates were closed and incubated at 37 degree Celsius for 24 hours and the diameter of inhibitory zone was calculated in millimeters. Each experiment was performed in five replicates. Both plant extracts showed considerable activity against gram positive bacteria. However, only ginger extract showed activity against Escherichia coli. Plant extracts showed no activity against Salmonella typhi. Petroleum ether and chloroform extract of ginger showed more activity against gram positive bacteria and methanol and water extract of ginger showed more activity against gram negative bacteria.
Availability: Items available for loan: UVAS Library [Call number: 0993,T] (1).
22.
Efficacy Of Florfenicol Against Haemorrhagic Septic Aemia In Bufalo Calves
by Shoaib Ashraf | Dr.Muhammad Ovais Omer | D. /Sjeruar Afzal | Prof. Dr. Azhar Maqboo.
Material type: ; Format:
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Publisher: 2008Dissertation note: Two antibiotic preparations of florfenicol and amoxicillin were used in these trials against haemorrhagic septicaemia, singly and along with combination of a non-steroidal-anti-inflammatory-drug (flunixin meglumin).
Forty buffalo calves suffering from haemorrhagic septicaemia were selected and treated under field conditions. Weighed clinical score was recorded before and after treatment in each case on the basis of severity of clinical symptoms. Reduction in this score and recovery or death of animal was also noted.
It was concluded that florfenicol was more effective than amoxicillin and the
n of non-steroidal-anti-inflammatory-drug reduced convalescence period and also jincreased the survival rate. Therefore florfenicol along with flunixin meglumin is ecommended as the successful therapy for haemorrhagic Septicaemia.
Availability: Items available for loan: UVAS Library [Call number: 1004,T] (1).
23.
Comparative Pharmacokinetics Of Metronidazole In Healthy Volulteers & In Patients Suffering From Amoebiasis.
by Bilal Ashiq | Prof.Dr.Muhammad Ashraf | Muhammed | Muhammed Imran Khokar.
Material type: ; Format:
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; Nature of contents: ; Literary form: Publisher: 2008Dissertation note: This study was designed to compare the pharmacokinetic parameters of Metronidazole in healthy volunteers and in human patients suffering from amoebiasis (target individuals). The study was conducted in six healthy male volunteers and six male patients suffering from amoebiasis in Services Institute of Medical Sciences (SIMS) Lahore. Only those patients were selected who were suffering from amoebiasis (confirmed after Stool test) between the age of 25-40 years. Healthy volunteers were also between ages of 25-40 years. The healthy volunteers were considered as group A and patients were considered as group A. 13oth groups were treated with Metronidazole 500mg orally per individual. 5m1 Blood samples were collected at 0, 0.25, 0.5, 1, 2, 3, 6, 12, 24 & 36 hrs after oral administration of Metronidazole. Plasma was separated by centrifugation at 5000 RPM and stored at -20°C until assayed. Metronidazole concentrations in plasma were measured by HPLC method. Pharmacokinetics parametes were calculated from plasma concentration time profile data. Pharmacokinetics of Metronidazole in healthy volunteers and in amoebiasis patients was compared. Data was analyzed by statistical methods (T Test) and it was observed that there was no significant difference in pharmacokinetic parameters of Metronidazole in healthy volunteers and in amoebiasis patients after oral administration and there is no need of dose adjustment for Metronidazole in amoebiasis patients.
Availability: Items available for loan: UVAS Library [Call number: 1024,T] (1).
24.
Comparative Pharmacokinetics Of Levofloxacin In Healthy Volunteers & Patients Suffering From Typhoid Fever
by Muhammed Usman | Prof.Dr.Muhammad Ashraf | Dr. Muhammad Imran Khokhar | Dr. Shehryar.
Material type: ; Format:
print
Publisher: 2008Dissertation note: This study was designed to compare the pharmacokinetic parameters of Levofloxacin in healthy volunteers and in human patients suffering from typhoid fever (target individuals). The study was conducted in six healthy male volunteers and six male patients suffering from typhoid fever in Services Institute of Medical Sciences (SIMS) Lahore. Only those patients were selected who were suffering from typhoid (confirmed after widal test) between the age of 25-40 years. Healthy volunteers were also between age of 25-40 years. The patients were considered as group A and healthy volunteers were considered as group B. Both groups were treated with Levofloxacin 5 00mg tab orally per individual. 5m1 Blood samples were collected at 0, 0.25, 0.5, 1, 2, 3, 6, 12, 24, 36 & 72 hr from vein through 5m1 B.D syring of 23guage needle after oral administration of Levofloxacin. Plasma was separated by centrifugation at 5000 RPM and stored at -20°C until assayed. Levofloxacin concentrations in plasma were measured by previously described HPLC method. Calculation of all the pharmacokinetic parameters was done by entering plasma concentration-time data in software APO pharmacological analysis MW/PHARM version 3.02 by assuming bio-availability of levofloxacin after oral administration as 1. Pharmacokinetic parameters of Levofloxacin in healthy volunteers and in typhoid patients were compared. Data was analyzed by appropriate statistical methods and it was observed that there is no significant difference in pharmacokinetic parameters of Levofloxacin in healthy volunteers and in typhoid patients after oral administration and there is no need for dose adjustment of Levofloxacin in typhoid patients.
Availability: Items available for loan: UVAS Library [Call number: 1027,T] (1).
25.
Study Of Hypoglycemic & Antihyperlipidemic Effect Of Ethanolic Extract Of Gymnema Sylvestre
by Shagufta Perveen | Prof.Dr.Muhammad Ashraf | Dr.Muhammed Ovais Omer | Muhammed.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2008Dissertation note: Gymnema sylvestre (Gurmar booti) is a herb native to the tropical forests of southern and central India. This project was designed to study the hypoglycemic and antihyperlipidemic effect of ethanolic extract of Gymnema sylvestre in alloxan induced diabetes in rabbits. For this purpose thirty rabbits were divided into six groups i.e. A, B, C, D, E and F having 5 rabbits in each group. Group A was taken as control and no treatment was given to control group. Group B was taken as control group for hyperlipidemia and it was treated with cholesterol powder at a rate of 600mg/Kg body weight orally daily for 15 days. Group C was treated with cholesterol powder at a rate of 600mg/Kg body weight orally daily to produce hyperlipidemia after 5 days this Group was treated with ethanolic extract of Gymnema sylvestreat at a rate of 400mg/Kg body weight orally daily for 15 days. In other groups diabetes were induced by treating them with alloxan at a rate of 150mg/Kg body weight intravenously into the marginal ear vein. The rabbits were monitored for a week for the development of diabetes by measuring glucose level, then they were treated. The Group D was taken as diabetic control group. Group E was treated with glibenclamide at a rate of 600p.g/kg body weight orally for 15 days. Group F was given ethanolic extract of Gymnema sylvestre at a rate of 400mg/kg body weight daily for 15 days. After the administration of ethanolic extract of Gymnema sylvestre, the animal was held in the wooden holder and 2 ml of the blood was obtained from the juglar vein with the help of syringe and serum glucose level and different biochemical parameters of lipid profile were estimated with the help of a UV-spectrophotometer. The samples were collected at 0, 5, 10 and 15 days. On the basis of results it was concluded that ethanolic extract of Gymnema sylvestre has a significant hypoglycemic effect in diabetic rabbits and their effect was comparable to that of gilbenclamide. Therefore, this medicinal plant is considered to be effective and alternative treatment for diabetes. Ethanolic extract of Gymnema sylvestre also has a significant antihyperlipidemic effect.
Availability: Items available for loan: UVAS Library [Call number: 1041,T] (1).
26.
Pharmacokinetics Of Meloxicam In Different Species
by Khawaja Tahir Mahmood (2003-VA-207) | Prof.Dr.Muhammad Ashraf | Dr. Mansoor Ud Din Ahmad | Dr. Makhdoom Abdul Jabbar.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2009Dissertation note: Meloxicam is a non-steroidal anti inflammatory drug. It has been registered in Pakistan as a safe substitute of diclofenac sodium which was banned for veterinary use due to its relay toxicity associated with the catastrophic decline in vulture population of the subcontinent.
Marked inter-species differences in the pharmacokinetics of NSAIDs have been reported and pharmacokinetic data cannot be extrapolated from one to another species.
The project was designed to study the pharmacokinetic parameters of meloxicam in domestic animals under local conditions. The study was completed in two phases. A simple, precise and accurate HPLC analytical methods for measurement of meloxicam in biological fluids was standardized and validated in phase-I. The pharmacokinetic parameters and absolute bioavailability of meloxicam in dogs were investigated in phase-I whereas pharmacokinetic parameters of meloxicam were characterized in the horses, donkeys, buffalo, and goats in phase-Il.
Eight healthy animals of each species (dogs, horses, donkeys, buffalos and goats) were used for this project. The dose for these animals was chosen from literature in order to achieve plasma concentrations of meloxicam that were likely to have an effect against inflammation.
The samples of blood were drawn as per protocol before and after medication at various time intervals. The separated plasma was stored at -20 °C till analyzed.
Availability: Items available for loan: UVAS Library [Call number: 1073,T] (1).
27.
Effect Of Two-Stroke Auto Rickshaw Smoke Solution On Wound Healing In Mice
by Aqeela Iqbal | Prof.Dr.Muhammad Ashraf | Dr.Muhammad Ovais Omer | Prof.Dr.Zafar.
Material type: ; Format:
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Publisher: 2009Dissertation note: The use of 3-wheel auto-rickshaws powered by a 2-stroke engine is widespread in south Asia; exhaust from these vehicles may cause different types of toxicities resulting in different pathologies. The aim of this study was to explore the association between exposure to 2-stroke auto-rickshaw smoke solution (2SARSS) and wound healing. The in vivo model of wound healing was customized to evaluate different stages of wound healing. A total of 56 mice were used of aged 4-5 weeks. After anesthetizing and hair removing from dorsum side of mice a wound of 3mm diameter was generated with the help of a sterile wound puncture. Smoke was collected in the lOOml distilled water from six 2-stroke rickshaws and then these solutions were filtered by syringe filters. 10% ointment of all the samples was prepared in Petroleum Jelly and applied to the experimental group, petroleum jelly to positive control group, no treatment was applied to negative control. Macroscopic evaluation was done by using high resolution camera photographs and measurement of wound by vernier caliper on alternate days. At the end of the study (day 12) histological evaluation was done when the control group wounds were healed. Histological examination revealed a significant delay in the outcome of re-epithelialization, dermal matrix regeneration, and maturation of collagen bundles among all 2SARSS-exposed wounds. Delayed activation of neovascularization was seen in the 2SARSS-treated groups at day 12 post-wounding. The Abbot curve, angular spectrum, and several other 3D surface parameters of reverse wound topographies revealed a highly significant reduction (p<O.OOl) in angiogenesis. These results demonstrate that application of 2SARSS causes a substantial delay in the progression of angiogenesis, resulting in delayed onset of wound healing. These observations validate the damaging effects of 2SARSS on wound healing. Thus, people who are directly or indirectly exposed to this toxic exhaust are expected to have delayed wound healing, which could result in chronic wounds.
Availability: Items available for loan: UVAS Library [Call number: 1083,T] (1).
28.
Comparative Efficacy Of Various Antiviral Agents Against Newcastle Disease Virus
by Shahzada Khurram Syed | Dr.Muhammad Ovais Omer | Dr.Aftab | Prof.Dr.Muhammad Ashraf.
Material type: ; Format:
print
; Nature of contents: ; Literary form: Publisher: 2010Dissertation note: In this project, 120 embryonated broiler chicken eggs of 9th days were obtained from Hi-tech laboratories Pvt Ltd and were placed in WTO quality control laboratory. Eggs were candled for separation of live embryos. For each drug to be tested, embryonated eggs were divided in 8 groups and marked them with lead pencil.
Eggs were disinfected with 70% ethanol. The New castle disease was virus obtained from Department of Microbiology, University of Veterinary and Animal Sciences, Lahore. Virus was identified by haemagglutination inhibition test. To prepare inoculums, stored virus was admixed at room temperature with antibiotics. Each embryonated eggs were inoculated with 0.1 ml of inoculum with the lml of BD syringes.
Eggs were incubated for 72 hours with frequent candling after every 24 hours. After, 72 hours of post inoculation, all live and dead embryos in eggs were kept in refrigerator at 4°C to 8°C. After, 72 hours antiviral groups were checked for the replication of ND virus by means of spot haemagglutination test. Drug's toxicity was checked for the viability of embryo by candling.
Three different concentrations of plant extracts of garlic, glycyrriza and ribavirin in normal saline were evaluated for' their antiviral activity and toxicity in embryonated eggs. Three concentrations for glycyrrhiza 1 5mg/iOOml, 30mg/i OOml and 60mg/i OOml were used. l5mg/lOOmi was neither toxic nor against virus. 6Omg/lOOmi was toxic and killed the embryos. Only 3Omg/lOOmi was antiviral as well as non-toxic.
Three different of concentrations of garlic were lgm/lOOmi, 5gm/lOOmi and 10gm/lOOm!. lgm/lOOmi was not having antiviral activity and it did not stop virus replication. 5gm/lOOmi and lOgm/lOOmi stopped virus replication and also non-toxic.
Three different concentrations 5ig/m1, lOtg/ml, 2Oig/ml of ribavirin were used. The first concentration was not having toxicity and antiviral activity. Second concentration was antiviral as well as non-toxic. Third concentration was toxic and killed the embryos.
This project was designed to investigate the efficacy of ribavirin and plant extracts (garlic and glycyrrhiza) against new castle disease virus and to reduce the cost of antiviral medicines and to explore flora of Pakistan.
Availability: Items available for loan: UVAS Library [Call number: 1104,T] (1).
29.
Effect On Lansoprazole On Antibacterial Activity Of Clarithromycin And Tetracyclin
by Samreen Faisal | Prof.Dr.Muhammad Ashraf | Dr.Aqeel Javeed | Dr.Sualeha Riffat.
Material type: ; Format:
print
Publisher: 2010Dissertation note: The study was designed to evaluate the effect of antibacterial drugs in the presence of lansoprazole (proton pump inhibitor) to determine the inhibition of bacterial efflux pumps. Resistance through efflux pump is one of the major mechanisms of resistance in bacteria. Bacillus ,subtilis, Salmonella typhi and Escherichia coli were isolated from clinical specimen and identified by using different microbiological techniques. Bacteria were inoculated separately in nutrient agar and their susceptibility for tetracycline and clarithromycin were checked by using Kirby baur disk diffusion method and the diameter of inhibitory zones (DIZ) was measured and analyzed by Unianova. The media were inoculated with three different concentrations of lansoprazole 1X, 2X and 3X separately and the same experiments were performed with each of the lansoprazole concentrations. The experiment was performed in five replicates. The diameter of inhibitory zone of tetracycline and clarithromycin in the presence of lansoprazole was measured in millimeters with the help of a scale and compared with the previously measured diameter of inhibitory zone of bacteria in absence of lansoprazole. Results of study showed that with increasing concentration of lansoprazole diameter of zone of inhibition of antibacterial drugs increases. The data suggest that the lansoprazole dose dependently increased the zone of inhibition of antibacterial drugs in the three test organisms used in study.
Availability: Items available for loan: UVAS Library [Call number: 1091,T] (1).
30.
Potentiation Of Fluoroquinolones By The Use Of Promethazine As Efflux Pump Inhibitor
by Rabia Altaf | Prof.Dr.Muhammad Ashraf | Dr.Sheryar Afzal | Prof.Dr.Khushi Muhammad.
Material type: ; Format:
print
Publisher: 2009Dissertation note: This study was conducted to demonstrate the potentiating effect of promethazine, an efflux pump inhibitor, on the sensitivity of Streptococcus pyogenes, Staphylococcus aureus, and Escherichia coli against fluoroquinolones. The bacteria were isolated from the field and were identified by using different microbiological techniques. The sensitivity of these bacteria was tested against four Fuoroquinolones i.e. ciprofloxacin, levofloxacin, norfioxacin and pefloxacin by using Kirby Bauer method. Diameters of inhibitory zones were measured in millimeters and all the tests were performed in five replicates. The same experiment was revised with the addition of Promethazine in concentrations of 64.tgIml, 128j.tg/ml, 192J1g1m1 and 256.tg/ml to the Petri plates separately. Diameters of inhibitory zones were measured and were compared with the negative control. The diameters of inhibitory zones of Staphylococcus aureus against ciprofloxacin(5 tg), levofloxacin(5 .tg), norfloxacin( 1 Oj.tg) and pefloxacin(5 .tg) alone were 14.6 mm, 20.4mm, 11.2 mm and 13.2mm but in the presence of promethazine in 256ig/ml concentration, the zones were 47.6mm, 39mm, 42.2mm, 35.8mm respectively. The diameters of inhibitory zones of Streptococcus pyogenes against ciprofloxacin(5 j.ig), levofloxacin(5 jig), norfloxacin( 1 Ojig) and pefloxacin(5 jig) alone were 22.4mm, 20.6mm, 15.0mm and 16.8mm but in the presence of promethazine (256j.tg/ml) the diameter of inhibitory zones were 40mm, 41mm, 37.8mm, 41.4mm respectively. The diameters of inhibitory zones of Escherichia coil against ciprofloxacin(5 jig), levofloxacin(5 jig), norfloxacin(lOjig) and pefloxacin(5 jig) alone were 23.2mm, 19.6mm, 20mm and 17mm but in the presence of Promethazine (256gig/ml) the zones were 42mm, 39mm, 43mm, 35mm respectively.
The increase in the diameter of inhibitory zones of bacteria against fluoroquinolones measured first in the absence and then in the presence of promethazine was found to be significant with P value less than 0.05. the results also demonstrated that this increase in the diameter of inhibitory zones was related to the increasing dose of promethazine, indicating that the increase in the susceptibility of bacteria for fluoroquinolones was a result of inhihition of bacterial efflux pumps by promethazine.
Availability: Items available for loan: UVAS Library [Call number: 1107,T] (1).
31.
Disposition Kinetics Of Oxytetracycline In Sheep
by Rana Fatima | Dr. Muhammad Nawaz | Mr. Riaz ul Haq Siddique.
Material type: ; Format:
print
Publisher: 1985Dissertation note: The pharmacokinetic s of ooxytetracycline was investigated in eight normal adult female sheep after intravenous administration. The blood samples collected at various time intervals were assayed for unchanged oxytetracycline. The plasma concerntrations of oxytetracycline at different time intervals from 5 minutes to 24 hours ater injection were plotted on semilograthmic graph paper versus time. The results are expressed after performing two compartment model kinetic analysis. After single intravenous administration of ox tetracycline in sheep, the distribution half life and biological half life shoed average +- values of 21.52 + 5.72 and 413.75+ 25.08 minutes and ranged between 16 to 29 manures and 365 to 442 minutes, respectively. The apparent volumes of distribution of oxytetracycline in sheep calculated by area method was 1.049+ 0.0009 1/kg and ranged between 0.99 to 1.25 1/kg-1. The average + values of volume of central compartment was 0.659+ 0.005 1/kg. The rate constant for oxyteyracyline transfer across compartment 1 to 2 (k12)and 2 to 1 (k21) were an average + SEM 0.723+0.02, 1.281 + 0.03 respectively. Total body clearance of oxytetracyline through intravenous route calculated on per kg basis ranged between 91.56 to 122.88 ml. min h-1 oxytetracylcine was bound to plasma protein on an average 49.3+ .002 percent and ranged between 46 to 51 percent. The renal clearance of total and free oxytetracycline as 5.42 + 2.09 and 10.57 + 3.81 ml/min 10 kg, respectively. The results showed that the renal clearance of oxyytetracylcine involved molecular filtration and back diffusion which is independent of pH, diuresis and presence of total and free oxtetracylcine. The average + SD urinary excretion of oxytetracycline as about 18.01 + 4.03 percent of the does at 24 hourse.
Availability: Items available for loan: UVAS Library [Call number: 1144,T] (1).
32.
Comparative Pharmacokinetics Of Carvediolol In Healty Male And Female Volunteers
by Alishba syed | Dr. Sualeha Riuffat | Prof. Dr. Muhammad Ashraf.
Material type: ; Format:
print
Publisher: 2010Dissertation note: This study was designed to compare the pharmacokinetic parameters of Carvedilol in 6 healthy male and female volunteers. The study was conducted in six healthy male volunteers and six healthy female volunteers. Only those male volunteers were selected who were of age between 18-30 years, not suffering from any disease. Female volunteers were also between age of 18-30 years, who were not pregnant and not suffering from any disease. Written consent was taken from them and they were be informed about objectives of the study, frequency of blood sampling, and possible side effects of drug which they might face during the study. The male volunteers were considered as group A and healthy female volunteers were considered as group B. Both groups were treated with Carvedilol 12.5mg tablet per orally to each individual. 5m1 Blood samples were collected at 0, 0.25, 0.5, 1, 1.5. 2, 3, 4, 6, 8, 12 & 24 hr from vein through 5ml B.D syringe of 22guage needle after oral administration of Carvedilol. Plasma was separated by centrifugation at 5000 RPM and stored at -40°C till analysis. Carvedilol concentrations in plasma were measured by HPLC method. All pharmacokinetic parameters were calculated by entering plasma concentration-time data in software APO pharmacological analysis MW/PHARM version 3.02. Pharmacokinetic parameters of Carvedilol in healthy male volunteers and in healthy female volunteers were compared. Data was analyzed byapproprite statistical methods and no significant difference was found between AUC and Cmax. Absorption rate was highr in females as compared to males. AUC of Carvedilol was 0.076±0.021 µg.h/ml in healty male voluneteers and 0.197±0.105 µg.h/ml in healty female volunteers. The half life was 5.205±1.824 hours in healty male volunteers and 6.6768±1.328 hours in female volunteers. The Cmax was observed as 0.024±0.004 µg.h/ml in healty volunteers and 0.048±0.018 µg.h/ml in healty female volunteers.
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33.
Pharmacokinetic Studies Of Amoxcillin Trihydrate In Healthy Buffalo Calves
by Amir Rashid | Dr. Sheryar Afzal | Prof. Dr. Muhammad Ashraf.
Material type: ; Format:
print
Publisher: 2010Dissertation note: The present study was designed to determine and compare the pharmacokinetic parameters of Farmox (Test Product) and Amoxi-vet (Reference Product) in healthy buffalo calves. For this study sixteen healthy buffalo calves between the ages of 6 to 12 months were purchased from the local market. They were kept in the shed of University of Veterinary and Animal Sciences Lahore. Their health status was monitored by physical examination. All the animals were maintained under similar conditions. The calves were provided fodder and water. All the animals were dewormed with Albendazole and a fifteen days wash out period was observed after deworming. The study was cross over design. Calves were divided into two groups A and B having eight animals in each. In the first phase calves of group A were administered Farmox (Test Product) intramuscular at the dose of 15mg/kg body weight and calves of group B were administered Amoxi-vet (Reference Product) intramuscularly at the dose of 15mg/kg body weight. In the second phase of the study, after a washout period of 15 days, group A were administered Amoxi-vet (Reference Product) and group B received the treatment with Farmox at same dose rate. Then 5ml blood was collected by direct pricking of jugular vein with needle and the blood was collected in heparinized test tubes. Prior to drug administration control/blank 5ml blood sample was also collected from each animal. After the drug administration the blood samples were collected at 0.166, 0.33, 0.50, 0.75, 1.0, 1.5, 2.0, 3.0, 4.0, 6.0, 8.0, 12.0, 24.0, 36.0 and 48.0 hours. The blood samples were then centrifuged at 4000 rpm for 10 minutes. Plasma was separated in small capped plastic bottles and measured by high performance liquid chromatographic (HPLC) method. Calculation of all the pharmacokinetic parameters was done by entering plasma concentration-time data in software APO pharmaceutical analysis MW/PHARM version 3.02. Pharmacokinetic parameters of Farmox and Amoxi-vet were compared. Data was analyzed by appropriate statistical methods and it was concluded that there is no significant difference in pharmacokinetic parameters of Farmox (Test Product) and Amoxi-vet (Reference Product) after intramuscular administration and both products are bioequivalent in their rate and extent.
Availability: Items available for loan: UVAS Library [Call number: 1201,T] (1).
34.
Effect Of Strawberry Juice On Angiogenesis Using Chorioallantoic Membrane (Cam) Assay
by Sadia Abrar | Dr. Muhammad Ovais Omer | Prof. Dr. Muhammad Ashraf.
Material type: ; Format:
print
Publisher: 2010Dissertation note: Angiogenesis, the formation of new blood vessels, is a hallmark of almost all neoplastic and non-neoplastic degenerative diseases. Targeting angiogenesis with natural antiangiogenic compounds may lead to safe, effective and low cost budget therapies. Strawberries provide various vital natural substances which have a significant role in human health and disease prevention. In our study, we have focused on the effect of strawberry juice on angiogenesis using chicken chorioallantoic membrane (CAM) assay. Fresh fertilized eggs were taken, sprayed with 70% ethanol and incubated at 37 °C (humidity 55-60%). At day 5 of incubation a small window was made on each egg, 4-5 ml of albumen was removed, windows were sealed with sterile parafilm and eggs were returned to incubator. Strawberry juice was obtained from fully ripened strawberry fruits and various dilutions were prepared in distilled water. Filtered dilutions of the juice were used for experimental analysis and applied to the CAMs on day 6. of incubation. Macroscopic vascular changes were evidently observed among all treated CAMs on day 7 of incubation. Reduction in the total area and diameter of primary, secondary and tertiary blood vessels was observed after treatment with strawberry juice in concentration dependent manner. By using SPIP software 3D surface roughness measurements were carried out which clearly elaborated antiagiogenic effect of strawberry juice on CAMs. Strawberry juice inhibits angiogenesis, which is a common denominator shared by various major disease.
Availability: Items available for loan: UVAS Library [Call number: 1213,T] (1).
35.
Effect Of Diclofenac Sodium On Angiogenesis Using Chorio Allantoic Membrance (Cam) Assay
by Iradat hussain | Dr.Muhammad Ovais Omer | Prof.Dr.Habib | Prof.Dr.Muhammad Ashraf.
Material type: ; Format:
print
Publisher: 2010Dissertation note: Angiogenesis, the growth of new capillary blood vessels in the body, has much more importance in healing and reproduction. The body controls angiogenesis as there is a natural balance between growth and inhibitory factors in healthy tissues.
When this balance is disturbed, the result is either too much or too little angiogenesis. Abnormal blood vessel growth, either excessive or insufficient, is now recognized as a "common denominator" underlying many deadly and debilitating conditions, including cancer, skin diseases, age-related blindness, diabetic ulcers, cardiovascular disease, stroke, and many others.
Treating angiogenesis with anti-angiogenic compounds results in safe and effective anti-angiogenic therapies. In current experiment, focus was on the effect of Diclofenac sodium on angiogenesis using chicken chorio-allantoic membrane (CAM) assay. Fresh fertilized eggs were taken, sprayed with 70% ethanol and incubated at 37 °C (humidity 55-60%). At day 5 of incubation a small window was made on each egg, 4-5 ml of albumin was aspirated, windows were sealed with sterile Para-film and eggs were returned to incubator. Concentrations i.e. 0.7%, 0.5%, 0.3% were prepared in distilled water. Filtered concentrations of the Diclofenac sodium were used for experimental analysis and applied to the CAMs on day 7 of incubation. Macroscopic vascular changes were evidently observed among all treated CAMs on day 6 of incubation. There was reduction in total area and diameter of primary, secondary and tertiary blood vessels was observed after treating CAMs with Diclofenac sodium. By using SPIP (Scanning probe image processor) software, 3D surface roughness measurements, Sa (surface area ), Sq (root mean square), Ssk (surface deviation), Sku (peakedness), Sdr (ratio of increment of interfacial area of a surface over sampling area), Sci (ratio of void volume of the unit sampling area at core zone over root mean square deviation ), Sy (lowest valley), Sz (average absolute height), Ssc (arithmetic mean submit), Sdq (root mean square sloop), Spk (reduce summit height), Sku (kurtosis of surface) , Stdi (texture index), Sk (core roughness depth) were carried out which clearly elaborated anti-angiogenic effect of Diclofenac sodium on CAMs. Diclofenac sodium inhibits angiogenesis, which is a common denominator shared by various major disease.
Availability: Items available for loan: UVAS Library [Call number: 1250,T] (1).
36.
Method Validation & Pharmacokinetics Of Carvedilol In Healthy Volunteers
by Mamoona Tariq.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2011Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1272,T] (1).
37.
Bioequivalence Study Of Montelukast Tablets In Healthy Volunteets
by Sadia Amin | Dr. Sualeha Riffat | Prof. Dr. Muhammad Ashraf.
Material type: ; Format:
print
; Nature of contents: ; Literary form: Publisher: 2011Dissertation note: Objective of this bioequivalence study was to compare pharmacokinetic parameters
and to evaluate bioequivalence of two generic drug products. A multinational
company brand was compared with locally manufacture brand. It was a randomized,
single dose, two-period crossover study in which 12 volunteers were participated with
the age limit of 18-30yrs. These volunteers were selected according to different
inclusion and exclusion criteria and the study was conducted with one week washout
period. Each volunteer was one tablet of montelukast (reference or test) lOmg. 14
blood samples of 4-Sml collected at predefined time intervals i.e, 0, O.S, 1.0, 1.S, 2.0,
2.5, 3.0, 3.S, 4.0, 6.0, 8.0, 10, 12 and 24 hours .. Heparinized vacuette were used for
collection of blood samples. After sampling, blood samples were centrifuged
immediately to separate plasma and stored at -80°C till analyzed. Plasma montelukast
concentration was evaluated by using reverse phase - high performance liquid
chromatography (RP-HPLC) method. Potassium dihydrogen phosphate O.OSM at pH
3.5 with orthophosphoric acid in combination to acetonitrile (20:80) was used as
mobile phase. The wavelength of detector was set at 34Snm and flow rate was set to
2.0ml per min. Drug from plasma was extracted by de-proteinizing the plasma with
acetonitrile. 70 III injection volume was given to HPLC for analysis. For comparing
the pharmacokinetic parameters two compartment analysis was used and pair t-test was applied. Non compartmental analysis was used for evaluating pharmacokinetic
parameters to evaluate the both drugs were bioequivalent or not. 3 major parameters
of bioequivalence Cmax, AVC O-inf and AVC O-t were evaluated and they did not
show significant difference in between two formulations. Also the 90% confidence
interval values were within the limit. So, it was concluded that both the test and
reference drug were bioequivalent and test drug could be used interchangeably with
the reference drug.
Availability: Items available for loan: UVAS Library [Call number: 1311,T] (1).
38.
Pharmacoinetic Srudy Of Ketoprofen In Healthy Sheep
by Awais Ali | Dr. Sheryar Afzal | Prof. Dr. Muhammad Ashraf.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2011Dissertation note: Objective of this bioequivalence study was to compare pharmacokinetic parameters and to evaluate bioequivalence of two generic drug products. A multinational company brand was compared with locally manufacture brand. It was a randomized, single dose, two-period crossover study in which 12 volunteers were participated with the age limit of 18-30yrs. These volunteers were selected according to different inclusion and exclusion criteria and the study was conducted with one week washout period. Each volunteer was one tablet of montelukast (reference or test) 10mg. 14 blood samples of 4-5ml collected at predefined time intervals i.e, 0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 6.0, 8.0, 10, 12 and 24 hours. . Heparinized vacuette were used for collection of blood samples. After sampling, blood samples were centrifuged immediately to separate plasma and stored at -80°C till analyzed. Plasma montelukast concentration was evaluated by using reverse phase - high performance liquid chromatography (RP-HPLC) method. Potassium dihydrogen phosphate 0.05M at pH 3.5 with orthophosphoric acid in combination to acetonitrile (20:80) was used as mobile phase. The wavelength of detector was set at 345nm and flow rate was set to 2.0ml per min. Drug from plasma was extracted by de-proteinizing the plasma with acetonitrile. 70 µl injection volume was given to HPLC for analysis. For comparing the pharmacokinetic parameters two compartment analysis was used and pair t-test was applied. Non compartmental analysis was used for evaluating pharmacokinetic parameters to evaluate the both drugs were bioequivalent or not. 3 major parameters of bioequivalence Cmax, AUC 0-inf and AUC 0-t were evaluated and they did not show significant difference in between two formulations. Also the 90% confidence interval values were within the limit. So, it was concluded that both the test and reference drug were bioequivalent and test drug could be used interchangeably with the reference drug.
Availability: Items available for loan: UVAS Library [Call number: 1312,T] (1).
39.
Evaluation Of The Effeet Of Different Modalities Of Vitiligo
by Basit Zaheer | Prof.Dr.Muhammad Ashraf | Dr. Aftab | Dr. Aqeel Javeed.
Material type: ; Format:
print
Publisher: 2011Dissertation note: This study was conducted to find out the clinical pattern of Vitiligo in selected patients and to
evaluate and compare the commonly used modalities for its treatment. All the patients with
vitiligo presented to the Department of Dermatology Mayo Hospital, Lahore. A total of seventy
patients were registered and followed-up for four months for repigmentation therapy. The
present study was aimed to uncover the various expressions of melanocyte deficiencies in
vitiligo In our people and to evaluate and compare the commonly used modalities for its
treatment. No such study, comparing the psoralens with topical steroids at the same time was not
done before.
For repigmentation therapy patients were randomly divided into various treatment groups and
me followed for the response for four months. The treatment groups included were PUVA
(Psolarens Ultraviolet A), PUVASOL (Psolarens Ultraviolet from Sunlight), topical PUVA,
Topical PUVASOL, topical steroids, systemic PUVA plus topical steroid and topical PUVA plus
topical steroid. Two new modalities were included to find newer effective ways of treatment and
their possible side effects. Complete repigmentation of the vitiligo without damage to the rest of
the body was the goal of the treatment. Initial approach involved to make a definite diagnosis,
psychological assistance and other supportive interventions such as use of camouflage cosmetics
and sunscreens. The active treatment modalities that could be utilized included the topical use of
potent steroids or photochemotherapy for atleast 2 months followed an assessment for response
measurement. Novel interventions are required to increase patient compliance and a search for
better treatment combinations.
Availability: Items available for loan: UVAS Library [Call number: 1323,T] (1).
40.
Bioequivalence Study Of Deferiprone In Healthy Volunteers
by Naila Waheed | Dr. Sualeha Riffat | Prof. Dr. Muhammad Ashraf.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2011Dissertation note: The study was conducted with the aim of evaluating bioequivalence, relative
silability and efficacy of deferiprone manufactured locally (Ferinil, Global
aceutical, Pakistan) with a reference drug (Ferriprox, ApoPharma, Canada) in healthy
volunteers. It was a randomized crossover study enrolling 12 volunteers within age limit
g·55yrs and meeting the inclusion and exclusion criteria of the study, Each volunteer was
administered two tablets of deferiprone 500mg of both reference and test drug with a two-
washout period. Blood samples of about 5ml was collected at 0, 0.25, 0.5, 0.75, 1, 1.5, 5,4, 6, 8, 12 hour at predetermined time intervals and one sample was taken as control
giving first dose to volunteers. Heparinized vacuette was used for collection of blood
les. After sampling, blood samples was centrifuged at approximately 3000 rpm for 10
les and then stored at -80°C till analyzed. Plasma deferiprone levels were analyzed using
led High pressure liquid chromatography (HPLC) method. Pharmacokinetic parameters
calculated from plasma concentration time curve non-compartmentally and two-
artmental. After logarithmic transformation of data statistical comparisons of Cmax,
(0-1), AUC(o.oo) was calculated and appropriate statistical method was used for calculation. mean relative bioavailability was 104% and was proved to be bioavailable. The Cmax
(mean ±SD) for reference and test drug was 12.68 ± 4.91 and 14.41 ± 5.04 ug/ml,
ctively while average ± SD of AUCO-t and AUCO-inf of test and reference drug was 40.49
6,05 and 42.84 ± 18.47 ugh/ml and 38.63 ± 13.65 and 40.75 ± 14.17 ugh/ml. Average
(test/reference) of Cmax 90% CI was 0.9876-1.3125. Average ratio (test/reference) of
Co.190% CI was 0.9737-1.1150, and of AUCo-inf 90% CI was 0.9542-1.1343. Therefore both test and reference drug was fairly tolerated by volunteers and no adverse event was
detected. Hence, the average ratio of 90% confidence interval of AUCo-t and AUCO-inf was
0.9737-1.1150 and 0.9542-1.1343 that lie within the acceptable limit of (0.80 - 1.25) for
bioequivalence acceptance. Effectiveness of deferiprone depends on AUC instead of Cmax therefore the average ratio of 90% confidence interval of Cmax was 0.9876-1.3125 that lie
with the acceptable limit of WHO bioequivalence acceptance (0.75 - 1.33). ANOVA show
no significant variations among drug, period and sequence effect. Therefore, it was concluded
that Ferriprox was proved to be bioequivalent in healthy male Pakistani volunleers.
Availability: Items available for loan: UVAS Library [Call number: 1327,T] (1).
41.
Comparative Pharmacokinetics Of Silymarin In Healthy Male And Female Volunteers
by Farah Abid | Prof.Dr.Muhammad Ashraf | Dr. Mateen | Dr. Sualeha Riffat.
Material type: ; Format:
print
; Nature of contents: ; Literary form: Publisher: 2011Dissertation note: The study was designed to compare the pharmacokinetic parameters of Silymarin in 8
healthy male and 8 healthy female volunteers. Only those healthy volunteers were
selected who were of age between 18-45 years, not having any disease. Female's
volunteers were also of age of 18-45 years and also who were not pregnant and also not
suffering from any disease.
Written consent form were taken from the volunteer and they were thoroughly
inform about the study and objectives of study ,frequency of blood sampling, and any
other side effects linked to the drug which they might having during the study.
Volunteers were divided into two groups A and B respectively. Both groups
were given silymarin 200mg dose per oral to each individua1.5ml of blood samples were
drawn after different time interval .5 ,1,2,3,5,8 and 12 hr from the vein through 5ml BD
syringes of 22 gauge needle after oral administration of silymarin.
Plasma were separated by centrifugation at 5000 RPM and stored at -40 C till
analysis. Silymarin concentration in plasma was determined by using HPLC method. All
pharmacokinetics parameter were determined by entering the plasma concentration time
data in software APO pharmacological analysis .Then pharmacological parameters in
healthy male and healthy females were compared. Result showed that pharmacokinetic
parameters are significant & those parameters were AVC, Tmax, t1l2 & CI. This result
showed that there is a significant relationship between healthy male & female.
Availability: Items available for loan: UVAS Library [Call number: 1329,T] (1).
42.
Pharmacokinetics Of Ketoprofen In Domestic Animals
by Zaka-ur-Rehman | Prof.Dr.Muhammad Ashraf | Prof. Dr. Makhdoom Abdul Jabbar.
Material type: ; Format:
print
Publisher: 2011Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1427,T] (1).
43.
Evaluation Of Mutagenicity And Cytotocicity Of Ferst Line Anti Tuberculosis Drugs
by Riffat Fatima | Dr. Muhammad Ashraf | Dr. Imran Altaf | Dr. Muhammad Adil Rasheed.
Material type: ; Format:
print
Publisher: 2012Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1458,T] (1).
44.
Comparative Evaluation Of Mutagenicity And Cyhalothrin, Of Endosulfan, Lambda-Cyhalothrin,
by Umber Saleem | Prof. Dr. Muhammad Ashraf | Dr. Imran Altaf | Dr. Muhammad Ovais Omer.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2012Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1460,T] (1).
45.
Quality Evaluation Of Different Brands Of Ceftriaxone
by Sana Tariq | Prof. Dr. Muhammad Ashraf | Dr. Muhammad Adil Rasheed | Miss Huma.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2012Dissertation note: This study was designed to determine the physicochemical equivalence of selected brands of ceftriaxone sodium registered with the ministry of health government of Islamic republic of Pakistan. Out of 9 selected brands 3 were of lowest price distribution class, 3 from the intermediate price distribution class and remaining three from the highest price distribution class. For quality evaluation 3 parameters were selected which were physical, chemical and microbial. Physical characters analyzed were general appearance, pH, solubility and moisture content. Characters which determine the chemical equivalence were assay of active ingredient and percentage of impurities present in powdered drug. Both these parameters were quantified chromatographically using high pressure liquid chromatography. Clinical efficacy of selected brands of this valuable antibiotic was accessed by determining the minimal inhibitory concentrations against Staphylococcus aureus, Salmonella typhi, Klebsiella pneumonia and Escherichia coli.
Statistically all brands were significantly different from one another but all the parameters taken as quality indicators showed results within the range specified by united state pharmacopoeia.
None of selected brands of ceftriaxone sodium were found to be counterfeit or even substandard. Irrespective of difference in price, no visible variation was found among different quality assessment parameters, all samples showed compliance with the international pharmacopoeial standards. Through this study it can be concluded that the quality of ceftriaxone in Pakistan is well regulated, all the registered brands are up to the mark and irrespective of variation in price there is no variation in the quality of brands.
Availability: Items available for loan: UVAS Library [Call number: 1462,T] (1).
46.
Chemical Equivalence Of Different Brands Of Oxytertacycline Hydrochloride And Its Minimum
by Sadaf Hina | Prof. Dr. Muhammad Ashraf | Dr. Aftab | Dr. Muhammad Adil Rasheed.
Material type: ; Format:
print
; Nature of contents: ; Literary form: Publisher: 2012Dissertation note: This project was designed to study the chemical equivalence of various brands of Oxytetracycline hydrochloride (long acting, short acting & PVP) approved by the ministry of health and available in the local market for veterinary use. Oxytetracycline was measured by HPLC method developed and standardized in the laboratory. Limit of detection (LOD) and limit of quantification (LOQ) of the Oxytetracycline by HPLC assay method were determined. From stock solution of working standard (Oxytetracycline hydrochloride) different concentrations 0.05, 0.1, 0.5, 1.0, 10, 25, 50 and 100µg per ml were prepared for the determination of LOD. The LOD calculated was 0.100(µg/ml) and LOQ was 0.5 (µg/ml). Correlation coefficient was 0.99994050. Concentration of the active ingredient (Oxytetracycline hydrochloride) in all preparations was same as mentioned on the label except Oxytetracycline (74%), Terrasym PVP-100 (81%), and Onyx-LA (72%).
MIC of Oxytetracycline hydrochloride against following bacterial isolates determined by micro-broth dilution test was Bacillus subtilis (50µg), Staphylococcus aureus (100µg), Eschericiha coli (50µg), Salmonella enterica (1000µg) and Pasturella multocida (50µg).It showed that all these bacterial cultures have developed resistance against Oxytetracycline hydrochloride.
Availability: Items available for loan: UVAS Library [Call number: 1468,T] (1).
47.
Pharmaceutical Equivalence Of Different Brands Of Moxifloxacin Hydrochloride And Minimum Inhibitory Concentration
by Sarmat Tamjeed Afzal | Dr. Muhammad Adil Rasheed | Prof. Dr. Muhammad Ashraf.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2012Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1471,T] (1).
48.
Effects Of Lycopersicon Esculentum And Citrus Limon Juice On Angiogenesis
by Nausheen Saba Khalid | Dr. Muhammad Ovais Omer | Prof. Dr. Muhammad Ashraf.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2012Dissertation note: Lycopersiconesculentumand Citrus limonumare largely known for their anti cancer activity. Various investigations using various assays have been done regarding anti cancer activity of Lycopersiconesculentumand Citrus limonum. This study wasplanned to estimate the effects of LycopersiconesculentumandCitrus limonumjuice on angiogenesis using the chick chorioallantoic membrane model. LycopersiconesculentumandCitrus limonum wascollected, sliced to squeeze juice, centrifuged and then supernatant was collected. Different dilutions were prepared using distilled water. PH was adjusted in range of 6-7 and filtered using disposable syringe filter. Fertilized eggs wereobtained from local hatchery and sprayed with 70% alcohol then divided in to six groups containing five eggs in each group one group act as control. The eggs were incubated at 37C° and at60_65% humidity. On 4th day postincubationwindows were made in all eggs under strict aseptic conditions, albumin was aspirated (approximately 4 -5 ml) with a disposable syringe, sealed with sterilized adhesive tapes and kept in incubator for 24 hours.After 24 hours windows were opened, 200 ?l of each dilution waspoured on CAM with disposable syringe, sealed again with sterilized adhesive tapes and again kept in incubator till 6th day. After 24 hours pattern of development of new blood vessels were assessed in CAM by taking images with the help of digital camera.Decrease in length and diameter of primary, secondary and tertiary blood vessels were seen in a concentration dependent way. All images were quantified by using scan probing image processing software (IBM- Denmark). 3D surface roughness parameters were calculated that evidently showed antiangiogenic nature of tomato and lemon juice. All the calculated data was subjected to statistical analysis.
Availability: Items available for loan: UVAS Library [Call number: 1476,T] (1).
49.
Bioequivalence Study Of Atorvastatin Tablets In Human Volunteers
by Asif Ali Bokhari | Dr. Sualeha Riffat | Prpf. Dr. Muhammad Ashraf.
Material type: ; Format:
print
Publisher: 2012Dissertation note: Main purpose of this clinical trail was to evaluate bioequivalence parameters of two generic drug products. It was a randomized, single dose, two-period crossover trial. Study was done on 12 volunteers with the age limit of 18-45yrs. Inclusion and exclusion criteria was used for volunteers selection and the study conducted with two week washout period. Each volunteer received one tablet of atorvastatin (reference or test) 10mg. Almost 13 blood samples of 5ml were collected at predefined time intervals. Blood samples were collected in heparinized vacuette. Blood was centrifuged after sampling and stored at -80°C till analysis. Serum atorvastatin levels were examined by using high performance liquid chromatography (HPLC) method mobile phase was a mixture of Sodium di-hydrogen phosphate buffer and acetonitrile 65:35 v/v pumped at flow rate of 1ml/min at a wavelength of 260nm. Two compartmental analysis was used for evaluating bioequivalence parameters to evaluate the both drugs were bioequivalent or not .2 major parameters of bioequivalencepeak plasma concentration Cmax and area under the curve AUC 0-t were evaluated. By statistical analysis, 90% confidence interval for area under the curve AUC 0-t was found to be 0.5972 - 1.7093, it was not within the range (0.80 - 1.25) proving an in equivalence between the two products so it shows that area under the curve AUC 0-t for both drugs is not equivalent. By statistical analysis, 90% confidence interval for peak plasma concentration Cmax found to be 0.3840 - 3.6638., it was not within the range (0.80 - 1.25). So it is evident that peak plasma concentration Cmax for both drugs is not equivalent. So, it was concluded that both of the drugs were not bioequivalent. From the given data, it is concluded that both the drugs produced uncomparable results. So it can be concluded that Lipiget cannot be used in replacement of Lipitor.
Availability: Items available for loan: UVAS Library [Call number: 1480,T] (1).
50.
Cytocenetic Effects Of Anti-Breast Cancer Drugs, Cyclophosphamide, Doxorubicin, Cisplatin And
by Zainab Batool | Prof. Dr. Muhammad Ashraf | Dr. Imran Altaf | Dr. Muhammad Ovais Omer.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2012Dissertation note: In this study mutagenicity and cytotoxicity of the chemotherapeutic agents used in breast cancer were evaluated. The drugs included in this study were Cyclophosphamide, Doxorubicin, Cisplatin and 5-Flourouracil. They were tested alone as well as in combination for their cytogenetic effects. The mutagenicity of these drugs was tested by Ames test using two strains of Salmonella i.e. TA100 and TA98 with and without S-9 at different concentrations. While for cytotoxicity evaluation MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) colorimetric assay was selected. 96 well plate and BHK-21 cell lines were used to perform this assay.
This study indicated that cyclophosphamide was mutagenic ( 62.5 µg/plate) to TA 100 with S-9 but non mutagenic to TA 98 with and without S-9, while the concentration of 250µg/ml and above was found cytotoxic. Doxorubicin was mutagenic to TA 100 and TA 98 with and without S-9 at 1 µg/plate and above, while cytotoxic dose was 10µg/ml and above. 5-FU was found non mutagenic in this assay to both test strains with and without S-9 at all test concentrations, however it was found cytotoxic above 5µg/ml in MTT assay. Cisplatin showed mutagenicity to both test strains at 2µg/plate and above , while at 5µg/ml and above it was found cytotoxic.
When the combinations of these drugs were tested for cytogentic effects , it was found that the concentrations which were non mutagenic individually became mutagenic and cytotoxic when combined together.
Availability: Items available for loan: UVAS Library [Call number: 1481,T] (1).
