1.
Molecular Characterization Of Antimicrobial Resistance Genes In Salmonella Isolates From Diarrheic Calves
by Hania Zulfiqar | Prof. Dr. Tahir Yaqub | Dr. Abu Saeed | Miss. Sehrish Firyal.
Material type: ; Format:
print
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drama
Publisher: 2013Dissertation note: A number of infectious (bacteria, viruses, parasites) and non-infectious factors cause diarrhoea in calves. Salmonella bacteria are gram-negative and belong to the family Enterobacteriaceae. Salmonella infections in calves continue to be a major problem worldwide and are responsible of causing major economical losses. To avoid the consequences of disease caused by Salmonella drugs like pencillin, tetracyclines e.g, are given to cattle but it is observed that Salmonella show resistance against these drugs after certain period of time. Salmonella is the major causative agent of calf diarrhea. The antibiotic genes against tetracycline and ampicillin are present in slamonella isolates from calves which are suffering from diarrhea.
Aim of my study was 1) Salmonella isolation and investigation of the antimicrobial resistance gene from diarrheic calves and 2) Molecular analysis of antibiotic resistance gene of isolated salmonella species. For this purpose, salmonella antibiotic resistant isolates against ampicillin and tetracycline were selected. Antibiotic resistant plasmid genes were amplified by PCR. After gel electrophoresis the resulting fragments were sequenced through genetic analyzer.
After sequencing all the sequences were viewed in Chromas Lite 2.1.1 , Sample sequences were aligned with the reference sequences obtained from NCBI by using Mega 5.05 software. Alignment results show that there is no Single Nucleotide Polymorphism found in salmonella.
Availability: Items available for loan: UVAS Library [Call number: 1744,T] (1).
2.
Genetic Characterization Of Livestock Species Of Pakistan Through Dna Barcoding
by Madiha Booter | Dr.Ali Raza Awan | Dr. Abu saeed | Dr. Muhammad Imran.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2013Dissertation note: The interaction of livestock with ecosystem plays a vital role in sustainability of life. The demand of livestock products is rising day by day which is changing the relationship between livestock and natural resources. Livestock animals are playing a major role towards domestication and also contributing to fulfill human needs through meat and milk production for food industry, which generate big revenues. Pakistan is blessed with the world's best livestock species and there is a need to establish a well characterized system for the classification and identification of these important livestock species.
Mitochondrial DNA is of small size, constitutes a small fraction of the total of cell's genome and due to high rate of mutation, it is considered to be an ideal model to study evolutionary relationships. DNA barcoding is being used to characterize animals by using a standard region of mitochondrial DNA as a molecular marker. The study is designed to develop the DNA barcode for genetic characterization of livestock species of Pakistan which includes sheep, goat, cow, buffalo and camel.
Blood samples were collected from the selected livestock species. Primers were designed using primer designing free-ware software. The amplified PCR products weresequenced in both orientations by chain termination method. For data analysis,Chromas was used to read sequencing results. To study variation in all sequenced data, alignment tools were used from NCBI. Theblastnalignment tool available at NCBI is more reliable to give authentic results.The alignment results showed 100% homology with the reference sequences (No SNP or mutation was identified). The results can further be validated with the help of mass level sampling to rationalize the study at population level.Phylogenetic analysis indicated that COIDNA barcode region can be used to discriminate unknown samples of any of the species under consideration. The COIgene successfully cladded already reported sequences of the same species.
This study provided genetic data which help in species identification, to assess evolutionary pattern and genetic diversity. So, it will also be helpful to monitor legal or illegal trade of livestock species and to identify processed and unprocessed meat for quality assurance. Establishment of an elaborated DNA barcode system for livestock species will help to start taxonomic investigation and will lead towards to identify many new mammalian species of Pakistan.
Availability: Items available for loan: UVAS Library [Call number: 1752,T] (1).
3.
Estimation Of Various Adulterants In Milk Available In Local Market
by Farhan Tanveer | Dr. Muhammad Wasim | Dr. Abu Saeed | Ms. Sehrish Firyal.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1781,T] (1).
4.
Molecular Investigation Of K99 Enterotoxigenic Escherichia Coli
by Nida Javaid | Prof. Dr.Tahir yaqub | Dr. Muhammad wasim | DR.Abu Saeed.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1967,T] (1).
5.
Detection And Analysis Of Traces Of Ignitable Liquids On Burnt Substeates
by Abdul basit | Akhtar ali | Dr. Abu Saeed | Dr. Muhammad Wasim.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2007,T] (1).
6.
Genetic Study Of Myp6, Mpy7, And Myp8, Loci Of Myopia In Punjabi Families
by Maria Arshad | Prof.Dr.Masroor Elahi Babar | Dr. Abu Saeed | Dr. Ali Raza Awan.
Material type: ; Format:
print
Publisher: 2010Dissertation note: Myopia is a refractive abnormality of the eye in which the parallel light rays from an object at optical infinity are focused by the eye in front of the retina rather than on it. It may be syndromic or non-syndromic. An extreme genetic heterogeneity is associated with this disorder. This is the first experimental study on Myopia in Pakistan. So, investigating the loci of myopia here is very important because this disease is spreading day by day with prevalence rate of 36.5%.
Microsatellite markers have been proved as an efficient and powerful tool for discovering any diseased locus. So a panel of these markers was used in this study. Blood samples of various myopic families were collected from various areas of Punjab and their DNA was extracted with the standard protocol. The amplification of DNA was done with primers of microsatellite markers belonging to the loci MYP6, MYP7 and MYP8. Genotyping was done for linkage analysis through PAGE. Haplotypes were made manually by observing the alleles of all the individuals on the gel. The results showed potential linkage against MYP7 locus for the family Myo-3 with autosomal dominant mode of inheritance. This family belongs to the caste "Khawaja" and was enrolled from PCSIR Phase II, Lahore, Punjab. All the affected individuals carried the same allele that was not present in the normal subject. Later the LOD Score for this family was calculated and maximum LOD score came out to be 0.0803 at the marker D11S904 that showed very low percentage of linkage. This can be confirmed by extending the family by further sampling.
Availability: Items available for loan: UVAS Library [Call number: 1157,T] (1).
7.
Single Nucleotide Polymorphisms In Pouifi Gene And Its Association With Milk Production Traits In Pakistani Cattle
by Sadia Munir | Dr. Asif Nadeem | Dr. Abu Saeed | Dr. Muhammad Wasim.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2011Dissertation note: In farm animals, the primary focus of selection has been to improve milk yield. Milk field is a polygenic trait and a few potential candidate genes have been recognized.
Association studies have shown that POUlFl is related to many production traits in
domestic animals and is one of those candidate genes that are involved in milk
production. POUl F 1 encodes a pituitary-specific transcription factor. It is well
established that growth hormone (GH) released from pituitary gland plays an essential
role in growth, mammary gland development and lactation process. The bovine
POUlFl gene is of 15952 bp length having 6 exons. The genetic characterization of
the POUlFl gene to identify the SNPs as genetic markers and validation of these
potential markers by associating them with milk production traits has been performed.
A total 35 samples from Sahiwal and 30 from Holstein-Friesian cattle breeds were
sequenced for all 6 exonic portions of the POUlFl by using 6 sets of primers. A total
15 polymorphic sites in Sahiwal and 14 in Holstein-Friesian were identified from
these sequences. Out of total 15 SNPs identified in Sahiwal, 12 were in intronic
region and 3 were in exonic. Out of 14 SNPs identified in Holstein-Friesian, 10 were
in intronic and 4 were in exonic region. The sequences of the amplified POUl F 1 gene
fragments were aligned with the help of BLAST for SNPs identification. This study is
first step in finding some confirmed markers for milk yield in Sahiwal and Holstein-
Friesian cattle breed that can be used in future for selection and breeding programmes.
Availability: Items available for loan: UVAS Library [Call number: 1373,T] (1).
8.
Genetic Variability Of Sahiwal And Cholistani Cattle Breeds Of Pakistan Usin Mitochondrial D-Loop Sequences
by Sania Saeed | Mr. Tanveer Hussain | Dr. Abu Saeed | Dr. Muhammad Wasim.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2011Dissertation note: Pakistan is rich in cattle genetic resources. The phenotypic and genetic diversity of animal breeds in Pakistan is very vast. Efforts to manage and utilize these genetic resources efficiently are lacking due to lack of both awareness and weakness of Government institutions. The genetic data of dairy cattle breeds (Sahiwal and Cholistani) is not yet been studied for their genetic identification, conservation and to find the genetic diversity among them and it needs to be established. For this study the blood samples( 25 samples from each breed) were collected from their home tracts and livestock farms. Unrelated animals with typical phenotypic features known for Sahiwal and Cholistani cattle breeds were selected from their breeding areas and Government livestock farms. Blood samples from true representative individuals of Sahiwal breed were collected from Research Centre for the Conservation of Sahiwal Cattle (RCCSC), Jahangirabad, Khanewal, Semen Production Unit (SPU) Qadirabad and Barani Livestock Production & Research Institute (BLPRI), Kherimurat District Attock. Cholistani cattle samples were collected from Govt. Livestock Farm, Jugaitpeer, Bahawalpur. Sampling from siblings was avoided to minimize inbreed samples as it results in depleting of gene pool along with causing inbreeding depression.DNA was extracted and quantified with the standard protocol in Molecular Biology and Genomics Laboratory of Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore. Specific primers were designed by using special software i.e. Primer Fox for mitochondrial D-loop region from NCBI accession no. NC_006853.1.Primers optimization was done after primer designing and afterwards, PCR amplification was performed. Then sequencing of target fragments was carried out using Prism ABI 3130L sequencer and Analyser.Sequences were alligned with the help of software blast2sequence and SNPs were detected. It was found that ratio of transition mutation was higher than transversions i.e. 41 transition and 10 transversions. Sequences were analyzed and compared with already reported sequence of Mitochondrial DNA of Bosindicuss, Bostaurus, Bubalusbubalis, Canis lupus familiaris, Caprahircus, Equuscaballusisolate, Ovisaries and Cameliusdromedaries sequencesavailable at NCBI. Single Nucleotide Polymorphisms (SNPs) were then detected. A phylogenetic tree constructed using MEGA 5.1 software revealed that Pakistani, European and Asian cattle are genetically same but different from Buffalo.This work is very helpful about breed characterization of two cattle breeds (Sahiwal and Cholistani) and developing understanding about genetic architecture of cattle breeds as present study conclude that 52 SNPs were present in Sahiwal and Cholistani breed of Pakistan.
Availability: Items available for loan: UVAS Library [Call number: 1498,T] (1).
9.
Mutation Screen Of "Gamma-Aminobutyric Acid (Gaba)-A Receptor, Gamma 2" In Punjab Population
by Muhammad Javed Iqbal | Dr. Muhammad Wasim | Dr. Abu Saeed | Dr. Ali Raza Awan.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2012Dissertation note: Epilepsy is a formidable form of neural disorder that can impose its long lasting effect on person's life and development. To date, it lacks any effective therapy and is multistep disease strengthened by an overwhelming number of genetic and epigenetic mechanisms that streamline epileptic attacks. This particular study encompasses two major types of epilepsy, CAE and GTCS by targeting a GABRG2 gene. Mutation analysis of the coding exons (exon 3, 5 and 9) was performed by direct sequencing of GABRG2 in order to sought out complex biological entities in both types of epilepsies. GABRG2 is a molecule that has recently been characterized as the culprit for epileptic seizures onset. GABRG2 encodes GABA receptor that is fundamental inhibitory neurotransmitter in mammalian brain and is a ligand-gated chloride channels. This ligand-receptor coupling results in the inward shuttling of chloride ions through the channels and this hyperpolarizes the neurons, which induce the inhibitory effect of neurotransmitters. Direct sequencing of candidate gene "GABRG2" traced out a single polymorphic site in the exon 3 of the CAE as well as GTCS cases. However, this single nucleotide alteration is more commonly identified in childhood absence epilepsy patients as compared to the generalized cases. Silent mutation was identified at locus 27909 C>T of 46.66% of the total screened or analyzed cases.
Availability: Items available for loan: UVAS Library [Call number: 1500,T] (1).
10.
Variation In The Non Structural Nd3 Gene Of Hepatitis C Viruses Isolated From Local Hospital Samples
by Muzna Rana | Dr. Asif Nadeem | Dr. Abu Saeed | Mrs. Saeeda Awais.
Material type: ; Format:
print
Publisher: 2011Dissertation note: Hepatic C virus (HCV) infects approximately 4% of the population worldwide. HCV establishes a chronic infection of the liver that causes cirrhosis and can lead to the development of hepatocellular carcinoma. HCV is transmitted exclusively through direct blood-to-blood contacts between humans. Current drug therapies for HCV infection often fail to resolve viral infection; therefore, new approaches for treating HCV infection are crucial in controlling chronic HCV infection. The viral protease NS3 has been identified as an attractive target for anti-HCV drugs, because its activity is indispensable for processing many of the NS proteins of HCV. Although the NS3 helicase is a potentially attractive target for anti-HCV drugs, no helicase inhibitors have yet entered clinical trials. In this research we are going to isolate the NS3 region of HCV genome, from different HCV positive patients with the help of RT PCR. Then sequence these, and observe similarities in these sequences. The main objective of the present study is to determine variation in the non structural gene NS3 of hepatitis C viruses isolated from patients suffering from hepatitis and reporting to local hospitals. The ultimate objective would be to utilize this data generated in this study attempting to prepare vaccine against this virus.
Availability: Items available for loan: UVAS Library [Call number: 1507,T] (1).
11.
Assessment Of Genetic Diversity In Balochi And Rakhshani Sheep Breeds Of Balochistan Using Microsatellite Dna
by Abdul Wajid | Dr. Muhammad Wasim | Dr. Abu Saeed | Mr. Tanveer Hussain.
Material type: ; Format:
print
Publisher: 2011Dissertation note: Pakistan being agriculture based country has a great potential in livestock sector, it plays an important role in the economy of the country. Pakistan is rich in Animal Genetics Resource (AnGR) and has various breeds of sheep but lacking genetic data of these breeds which need to established data for their genetic identification. Customarily, classification of breed was based on phenotypic traits. In some cases, recent genetic studies have found differences in the structure proposed. Molecular characterization is a prevailing tool to consider the genetic variation existed within and among breeds. Characterization and evaluation of genetic differences among these breeds is necessary for their effective and meaningful improvement and conservation. The advent of molecular techniques has led to an increase in the studies that focus on the genetic characterization of domestic breeds using genetic markers. Due to their reliability and availability, the microsatellites have become preferred method for the genome mapping. Microsatellites or STRs are the 2-6 nucleotide tandem repeats present in both coding and non coding regions of both prokaryotes and eukaryotes. Microsatellites are powerful tools in genome mapping, forensic DNA studies, paternity testing, population genetics and conservation/ management of biological resources.
The present study was conducted on the molecular diversity analysis of two sheep breeds Balochi and Rakhshani of Balochistan using 11 FAO recommended microsatellites markers. Blood samples of unrelated true representative animals of sheep breeds were selected from their breeding tracts and from different Government Livestock Farms in Balochistan province. DNA was extracted with the standard protocol and amplification of DNA done with selected markers in Molecular Biology and Genomics Laboratory in the Institute of Biochemistry and Biotechnology. PCR products were examined on non denaturing Polyacralamide Gel Electrophoresis (PAGE). Genotyping results vanalyzed through the software POPGENE VERSION 1.31 and "POWER STATE" for calculating the observed and expected number of alleles, expected and observed heterozygosity, homozygosity, F-statistics (FST, FIT, FIS), Polymorphic Information Content (PIC), matching probability power of discrimination and power of exclusion. This work provided the genetic data which is useful in breed identification and making effective breeding policies and conservational activities in future according to FAO global Farm Animal Genetic resource data.
Average observed heterozygosity, average observed homozygosity, observed number of allels (na) and expected number of alleles for all loci and population in this study were 0.6055, 0.3945, 6.3636 and 4.2805 respectively. Almost all of the microsatellite markers showed significant variations in both breeds of sheep.
This work provided the genetic data which will be helpful in breed identification and making effective breeding policies and conservational activities in future according to FAO global Farm Animal Genetic resource data. Moreover this study can become the basis for further research investigations in sheep breeds in Balochistan and Pakistan.
Availability: Items available for loan: UVAS Library [Call number: 1516,T] (1).
12.
Linkage And Mutational Analysis Of Gene Lebercilin (Lca5) In Families With Leber Congenital Amaurosis
by Adeel Ahmad | Prof. Dr. Masroor Ellahi Babar | Dr. Abu Saeed | Mrs. Saeeda Kalsoom.
Material type: ; Format:
print
Publisher: 2012Dissertation note: Leber congenital amaurosis (LCA, MIM #204000) accounts for at least 5% of all retinal dystrophies and approximately 20% of children attending schools for the blind. LCA is the most severe retinal dystrophy causing blindness or severe visual impairment before the age of 1 year. Inheritance is autosomal recessive in most cases. Clinically, LCA is characterized by the presence of four key features, namely severe and early visual loss (usually around the age of 6 weeks), sensory nystagmus, amaurotic pupils, and minimal or absent responses on the electroretinogram (ERG).
A total of five families (LA01-LA05) were enrolled, blood samples were collected and processed for DNA extraction. During linkage and genome scan, single family showed linkage to LCA5 locus. The diagnosis was established in all affected individuals by medical history, funduscopy, and standard ERG. We performed genome-wide linkage analysis for mapping the disease locus in this family.
Congenitally severely reduced visual acuity and nystagmus were reported for all patients. LCA in the family cosegregated with homozygosity for a single nucleotide polymorphism (SNP) haplotype on chromosome 6p14.1. The respective candidate region contained Leber congenital amaurosis 5 (LCA5), a gene previously reported to underlie LCA; subsequently identified a novel truncating mutation in exon 4 of LCA5, c.642delC, in homozygous state in all affected persons of the family LA01. Here, a novel LCA5 mutation causing LCA in a Pakistani family is reported.
Availability: Items available for loan: UVAS Library [Call number: 1518,T] (1).
13.
Identification Of Polymorphism In Bone Morphogentic Protein Receptor Type-1B (Bmpr-1B) In Teddy Goats
by Sonia Noreen Anjum | Dr. Muhammad Imran | Dr. Abu Saeed | Ms. Sehrish Firyal.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2013Dissertation note: Teddy goats provide a great scope for enhancing meat and milk production being the primary objective to compensate for increased demand in Pakistan. It is an established fact that an animal producing twins or triplet contributes more than 1.5 times toward meat than the animals producing single offspring per kidding. Hence, the identification of major fecundity genes, mutations of which are thought to elevate ovulation rate and litter size in goats as well as sheep breeds, has been the center of attention for all scientists. Four major fecundity genes expressed in goat ovary namely: GDF-9, BMP-15, ESR-? and BMPR-1B are the causative genes for high prolificacy.
Bone morphogenetic protein receptor type-1B (BMPR-1B) gene first identified ingranulosa cells of ovary. A-G transition at 746 bp at the FecB gene locus causing an amino acid substitution namely Q249R increases the antral follicular maturation leading to the release of a large number of ovules hence increasing litter size in range from 1.4-2.7 kids/birth.
In this study, blood samples from 52 Teddy goats were collected having twining record and processed for DNA extraction. DNA fragments containing FecB gene were PCR-amplified from extracted DNA samples. The PCR amplicons containing Q249R substitution were subjected to RFLP so that the presence or absence of these polymorphisms could be analyzed.
On analysis with DdeI restriction enzyme, three types of allelic fragments namely: wild type, homozygous mutant and heterozygous mutant of FecB gene mutation in Pakistani Teddy goats were to be observed. Whereas,the results obtained for this study strongly suggests that the Q249R mutation of FecB marker in BMPR-1B gene was not present in Teddy goats and these goats were found to be non-carriers for this mutation having wild type alleles. However, this work did not claimed the absence of any other mutation in BMPR-1B. There may be the involvement of other fecundity genescausing the increased prolificacy of these goats causing twining and triplets namely: Growth differentation factor-9 (GDF-9) and bone morphogenetic protein-15 (BMP-15).
Availability: Items available for loan: UVAS Library [Call number: 1670,T] (1).
14.
Detection And Quantification Of Dna From Saaliva From Cigarette Butts In Different Genders
by Qurra-tul-Aien | Prof. Dr. Tahir Yaqub | Dr. Abu Saeed | Dr. Muhammad Imran.
Material type: ; Format:
print
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1675,T] (1).
15.
To Investigate The Morphology Of Lip Prints And Their Effectiveness In Individualization And Sex Determination
by Makhdoom Saad Waseem Ghouri | Dr. Muhammad Wasim | Dr. Abu Saeed | Prof. Dr. Tahir Yaqub.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1720,T] (1).
16.
Assesment Of Diacylglycerol-Acyltransferase-1 Gene Polymorphisms In Nili Ravi Buffalo For Milk Production Trait
by Muhammad Amir zaib khan | Dr. Asif Nadeem | Dr. Abu saeed | DR. Tanveer hussain.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1966,T] (1).
17.
Forensic Utilization Of Synthetic And Non Synthetic Powders And Liquid Soaps For Development Of Fingerprints On Adhesive Sides
by Sobia nosheen | Dr. Muhammad Ashraf tahir | Dr. Abu saeed | Dr. Muhammad wasim.
Material type: ; Format:
print
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1993,T] (1).
18.
Meca Gene Based Methicillin-Resistant Staphylococcus Aureus Typing Isolated From Poultry And Their Potential
by Muhammad Sohaib iftikhar baig | Dr. Sehrish Firyal | Dr. Abu Saeed | Dr. Muhammad Zubair Yousaf.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2082,T] (1).
