1.
Mercury Intoxiation In Rohu (Rabeo Rohita)
by Waseem, M | Dr. Shakil Akhtar Khan | Dr.Sharif Mughal | Dr.Zafar Iqbal | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2003Dissertation note: The present project was designed to study the effect of Mercuric chiorkie (TIgCI2) toxicity in Labeo rohita (Rohu). For this purpose 175- famred fish Laheo rohita were bought from a local hatchery, kept and managed at University of Veterinary and Animal Sciences. After one week of acclimatization fish were exposed to mercuric chloride for acute and chronic phase to different sub-lethal doses of mercury. For acute phase experiment 40 fish were divided in to 4 equal groups viz A, B, C and D. Groups B, C and D were given HgCI2 at sub-lethal dose rate of 0.06, 0.09 and 0.12 mg/I respectively while group A served as control group. Liver, skin and gills were collected after 48 and 96 hours for pathological studies.
For chronic phase experiment 50 fish were divided in five equal groups viz F, G, 11, 1 and 3 were given mercuric chloride (HgCI2) at sublethal dose rate of 0.04mg/i for 4, 8, 12, 16 and 20 days respectively. Whereas group E which served as control further subdivided into five groups viz Ei, E2, E3, E4 and E5 had 25 fish maintained in 5 aquaria. Liver, skin and gills were collected from each group after 4, 8, 12, 16 and 20 days respectively for pathological studies. In treatment groups normal histology of liver disturbed and there observed necrosis, pycnotic nuclei, cellular infiltration, congestion, degeneration, deformation and dilatation of ducts. Histology of skin showed changes like pycnosis, necrosis, deposition of salts, degeneration and vacuolization of epithelial cells. Histopathology of gills also showed a wide range of lesions like hypertrophy of lamellae, larnellar fusion, club shaped lamellae, dis-integration, congestion and hemorrhages. In chronic phase experiment effects of HgCI2 on clinical signs and growth rate of fish were also studied. Restlessness, difficult breathing, fin flickering and jerky movements were observed in treatment groups. Suppressed growth rate was also observed in treatment fish. It was concluded that mercury intoxication not only results in marked histopathological changes and abnormal clinical signs but also results in depressed growth rate.
Availability: Items available for loan: UVAS Library [Call number: 0805,T] (1).
3.
Passive Immunization Of Foot And Mouth Disease Virus Infected Bovines
by Sajjad Hussain | Dr.kushi Muhammad | Dr.Masood Rabbani | Dr.Zafar iqbal | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2003Dissertation note: In this study, passive immunization of Foot and Mouth Disease (FMD) virus infected bovines was studied. One-year-old buffalo calves were primed and boosted with an inactivated aluminized FMDV vaccine. It induced antiFMDV-serotype "0" and "Asia-1"-complement fixing (CF)-antibodies in their blood.
Antibodies against FMD virus serotype "0" (calculated-CF anti-body units) protected the FMD infected animals. It was observed that the animals receiving 1600, 800 and 400 CF-antibody units against FMD virus serotype "0" showed 100%, 100% and 25% protection in 24 hours respectively. The FMD infected animals (control group) receiving serum containing undetectable level of anti-FMDV-type "O"-CF-antibodies showed zero % protection. The serum samples of this group were found positive for antiFMDV-serotype "O"-CF-antibodies while negative for anti-FMDV-serotype "Asia-1"-CF-antibodies, conferring the outbreak of FMD virus serotype "0".
It was inferred from this study that the hyperimmune serum containing more than 800 units of anti-FMD V-type "O"-CF-anti-bodies can be used as therapeutic agent to cure the FMDV-type "0" infected animals.
Availability: Items available for loan: UVAS Library [Call number: 0824,T] (1).
