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1. Comparative Studies On The Sensitivity Of Polymerase Chain Reaction (Pcr) And Conventional Serological Methods For the Diagnosis of Bovine Brucellqsis

by Raheela Akhtar | Prof. Dr. Zafar Iqbal Chaudhary | Prof. Dr. Abdul Rauf Shakoori | Prof. Dr. Asim | Faculty of Veterinary Sciences.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2007Dissertation note: The polymerase chain reaction was standardized and its efficacy was evaluated against Rose Bengal Plate Test (RBPT) and Milk Ring test (MRT) for the diagnosis of brucellosis in 200 cows and buffaloes from Lahore and Okara districts of Punjab. Under aseptic measures 200 serum and 200 milk samples were tested by RBPT, MRT and PCR on both milk and serum samples in both cows and buffaloes as described in materials and methods. RBPT showed high sensitivity values (27.7% in cows and 45.2% in buffaloes) than serum PCR (25% in cows and 3 9.6% in buffaloes) but on other hands MRT showed low sensitivity (11.1% in cows, 25.4% in buffaloes) and high specificity (98.4% in cows and 93.6% in buffaloes) than milk PCR with sensitivity of 13.8% in cows, 29.4% in buffaloes and specificities of 95.2% in cows and 89.3% in buffaloes respectively. The comparison of PCR assays conducted on both types of samples showed high sensitivity of serum PCR against milk PCR. The comparison of RBPT and MRT in both species showed high sensitivity of RBPT than MRT. But due to low positive predictive value of RBPT and instability in its results in both species it is concluded that there is no significant difference in PCR and serological methods so no single test can be used for the exact diagnosis of bovine brucellosis. Availability: Items available for loan: UVAS Library [Call number: 0957,T] (1).

2. Antibody Resoinse Of Broilers To Oil Based Combined Mycoplasma Gallisepticum And Avian Influenza H9

by Sadi Sarfaraz | Prof. Dr. Khushi Muhammad | Prof.Dr. Asim | Prof.Dr.Tahir yaqub.

Material type: book Book; Format: print Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1976,T] (1).

3. Optimization Of Loop Mediated Isothermal Amplification Lamp Technique For The Molecular Diagnosis Of Surra In Domestic Animals

by Muhammad Saleem iqbal | Dr. Haroon akbar | Dr. Muhammad lateef | Prof. Dr. Asim.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2039,T] (1).



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