1.
The Diagnostic Value Of Tta Codon Substitution In Los Angeles Galactosemia
by Sahr Malik | Dr. Muhammad Imran | Dr. Muhammad | Miss. Sehrish Firyal.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2012Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1590,T] (1).
2.
Pcr (Polymerase Chain Reaction) Test Development And Its Application For The Diagnosis Of Congenital Leptin Deficiency
by Nida Fakhar | Dr. Muhammad Imran | Miss Faiza | Miss. Sehrish Firyal.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1383,T] (1).
3.
Identification Of Pesticide Residues In Different Vegetable Collected From Market Of Lahore, Pakistan.
by Anam Munawar | Dr. Muhammad Imran | Dr. Abu Saeed Hashmi | Dr. Muhammad.
Material type: ; Format:
print
Publisher: 2013Dissertation note: Pesticides are the chemicals which are used to kill or repel the unwanted objects such as pests. Different types of pesticides are present which undergo a different mechanism and kill the pests. Four different types are being used in Pakistan such as organophosphate, organochlorine, pyrehtroid and carbamates. Use of organophosphate and organochlorine become less due the presence of residues. Use of pesticides is increased for a number of purposes such as to increase the rate of production, to decrease the damage of crops and to increase the saving time of different vegetables.
Vegetables are the main source of income of Pakistan, and vegetables are common in our use. Vegetables contain different nutritional elements of our diets. That's why vegetables play an important role in the nutritious diet of a person. The spray of different chemicals on vegetables not only decreases the nutritional elements but also increase the risk of different diseases.
As pesticides leave their residues in vegetables, different techniques can be used to detect the residues and their maximum residue limit, at which limit these pesticides are harmful for humans. Pesticides can also act on unintended individual such as human beings and cause different acute and chronic diseases.
Different vegetables were selected for analyses that are common in use and available in every season. Pesticides which were selected are that which are common in Pakistan and from different pesticide classes.
In present study vegetables of different areas of Lahore were collected and analyzed through HPTLC and GC/MS. HPTLC was used to analyze and calculate the concentration and GC/MS was used for the confirmation of results, and it was concluded that which vegetable contain the high concentration of pesticides. It was studied that which vegetable absorb large amount of pesticides. Potato, tomato, egg plant, okra and cucumber of different markets of Lahore contain high concentration of pesticides as compared to the other vegetables.
Availability: Items available for loan: UVAS Library [Call number: 1510,T] (1).
4.
Diagnostic Value Of 4Bp- 5' Gtca Deletion In Duarte Galactosemia
by Sadia Zia | Dr. Muhammad Imran | Ms. Faiza | Ms. Sehrish Firyal.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1602,T] (1).
5.
Identification Of Pesticides Residues In Defferent Samples Of Milk
by Neelam Shahzadi | Dr. Muhammad Imran | Dr. Abu Saeed Hashmi | Dr. Muhammad.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1646,T] (1).
6.
Identification Of Polymorphism In Bone Morphogentic Protein Receptor Type-1B (Bmpr-1B) In Teddy Goats
by Sonia Noreen Anjum | Dr. Muhammad Imran | Dr. Abu Saeed | Ms. Sehrish Firyal.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2013Dissertation note: Teddy goats provide a great scope for enhancing meat and milk production being the primary objective to compensate for increased demand in Pakistan. It is an established fact that an animal producing twins or triplet contributes more than 1.5 times toward meat than the animals producing single offspring per kidding. Hence, the identification of major fecundity genes, mutations of which are thought to elevate ovulation rate and litter size in goats as well as sheep breeds, has been the center of attention for all scientists. Four major fecundity genes expressed in goat ovary namely: GDF-9, BMP-15, ESR-? and BMPR-1B are the causative genes for high prolificacy.
Bone morphogenetic protein receptor type-1B (BMPR-1B) gene first identified ingranulosa cells of ovary. A-G transition at 746 bp at the FecB gene locus causing an amino acid substitution namely Q249R increases the antral follicular maturation leading to the release of a large number of ovules hence increasing litter size in range from 1.4-2.7 kids/birth.
In this study, blood samples from 52 Teddy goats were collected having twining record and processed for DNA extraction. DNA fragments containing FecB gene were PCR-amplified from extracted DNA samples. The PCR amplicons containing Q249R substitution were subjected to RFLP so that the presence or absence of these polymorphisms could be analyzed.
On analysis with DdeI restriction enzyme, three types of allelic fragments namely: wild type, homozygous mutant and heterozygous mutant of FecB gene mutation in Pakistani Teddy goats were to be observed. Whereas,the results obtained for this study strongly suggests that the Q249R mutation of FecB marker in BMPR-1B gene was not present in Teddy goats and these goats were found to be non-carriers for this mutation having wild type alleles. However, this work did not claimed the absence of any other mutation in BMPR-1B. There may be the involvement of other fecundity genescausing the increased prolificacy of these goats causing twining and triplets namely: Growth differentation factor-9 (GDF-9) and bone morphogenetic protein-15 (BMP-15).
Availability: Items available for loan: UVAS Library [Call number: 1670,T] (1).
7.
Development Of The Test For The Diagnosis Of Classical Galactosemia In General Papulation
by Mehmmona Iqbal | Dr Muhammad Imran | Ms Faiza | Ms Sehrish Firyal | IBBT.
Material type: ; Format:
print
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1856,T] (1).
8.
Molecular Chracterization Of Pakistani Gaucher Disease Type 2 Patients From Lahore
by Maliha Afreen | Dr Muhammad Imran | Ms Asma Waris | Ms Sayeda Kalsoom | IBBT.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1857,T] (1).
9.
In-Silico Functional Prediction Analysis Of Prion Protein Polymorphisms In Sheep Scrapie
by Mubashar ahmad | Dr. Muhammad imran | Dr. Muhammad | Dr. Wasim shehzad.
Material type: ; Format:
print
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2012,T] (1).
10.
In Silico Functional Prediction Of Prion Protein Polymorphisms In Chronic Qasting Disease
by Iqra khizar | DR. Muhammad imran | Dr. Muhammad | Dr. Muhammad yasir zahoor.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2024,T] (1).
11.
A Studyof Pesticidues In Different Fruits Collected From Differentfruit Markets Of Lahore Punjab
by Muhammad shafi | Dr. Muhammad imran | Ms. Huma mujahid | Ms. Saeeda.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2037,T] (1).
12.
Variation Analysis Of Hepatitis C Virus Gene Encoding E2 Glycoprotein
by Saimoon Theeen (2009-VA-565) | Dr. Muhammad Imran | Dr. Sehrish Firyal | Dr. Muhammad Tayyab.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: Hepatitis C virus (HCV) major cause of liver infections was discovered in 1989. It is positive stranded RNA virus and belongs to Flaviviridae family. Its genome shows high rate of variations due to which, its rate of infection is high. As in Pakistan 3% to 6% population and 170 million people worldwide are affected by it. Due to variations in its genome it is classified into 6 major genotypes and these genotypes are further classified into many subtypes. Size of HCV is about 9500 bps which only encodes single polyprotein. This 3000 to 3300 amino acids polyprotein is processed by cellular and viral proteases to generate 10 polypeptides consisting of 4 structural (Core, E1, E2 and P7) and 6 non-structural proteins (NS2, NS3, NS4A, NS4B, NS5A, NS5B). Whereas E2 is considered the most immunogenic gene from all the genes. It involves in the interaction with the host cell and easily escape from the immune system of host due to the presence of hypervariable regions in E2 gene.
To isolate the E2 gene RNA extraction was done using the kit method and then it was converted to the cDNA which is then followed by the PCR amplification. The amplification products were then purify and sent for the sequencing to CAMB. Then the bioinformatics tools were applied on the results. In which the protein structural analysis and epitope mapping was done. Then the conserved epitopes were predicted using the IEDB conservancy analysis tool. The conserved B-cell epitopes (TElAILPCSFTPMPAL and RGERCDIEDRSEQH) and T-cell epitope (TPMPALSTG) are now considered valuable to produce the antibodies against E2 protein.
For diagnosing HCV genotype 3a, these conserved epitopes may be highly useful and may also help in developing a successful vaccine that can target 3a genotypes. Availability: Items available for loan: UVAS Library [Call number: 2333-T] (1).
13.
Mutational Analysis Of Hepatitis C Virus Ns4b Gene Encoding Protein
by Faiza Nisar Bukhari (2013-VA-12) | Dr. Muhammad Imran | Dr. M.Yasir Zahoor | Dr. Abu Saeed Hashmi.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: Hepatitis C virus (HCV) infection has an estimated global prevalence of 2%–3% with approximately 270 million infected people worldwide of which 10 million belongs to Pakistan. HCV is a positive-sense single-stranded RNA virus from the family of Flaviviridea. Its genome encodes 10 proteins including a 261 amino acids protein NS4B. NS4B is a non-structural protein that performs hyperphosphorylation of NS5A, transactivates interleukin 8 promotor, suppresses HCV translation and modulates the ER stress response. Mutation in NS4B encoding gene may be targeted to develop an effective vaccine against HCV before virus invasion in host immune system.
Serum was collected from 20 patients who are infected with HCV. RNA was isolated from these samples to reverse-transcribe it into cDNA. This cDNA were PCR-amplified and amplicons were detected by UV light after their separation through agrose gel electrophoresis followed by seguencing of NS4B encoding gene.
Variations in HCV NS4B gene was analyzed by using Mega 6 software and sequence 2 blast analysis NCBI tool. Protein modeling was performed with phyre 2, DIANNA tool and I-Tasser online server and quality of model and antigenicity was checked by Vaxajen V 2.0 and Epijen softwares. Conservative analysis and epitope mapping were performed by using Immune Epitope Database (IEDB).
A systematic approach was employed for the prediction of potential epitopes in NS4B protein. Vaxijen V 2.0 was used to determine overall antigenicity of NS4B using cut off value of 0.4. Values above this threshold level show probable antigens in the protein. The topology of NS4B was determined by TMHMM server with the help of membrane topology data. Regions outside membrane and transmembrane were eradicated for epitopes prediction. T cell epitopes propred was used with proteosome cleavage site filter of 4% threshold. From the following data analysis 12 to 15 possible T-cell epitopes can be predicted; only these T cell epitopes were included in conservation analysis.
The present study provides information about mutational changes in HCV protein NS4B and thus preliminary data for the development of vaccine against HCV. This can also lead to future prospects for diagnosis, treatment and prognosis of HCV.
Hepatitis C virus (HCV) infection has an estimated global prevalence of 2%–3% with approximately 270 million infected people worldwide of which 10 million belongs to Pakistan. HCV is a positive-sense single-stranded RNA virus from the family of Flaviviridea. Its genome encodes 10 proteins including a 261 amino acids protein NS4B. NS4B is a non-structural protein that performs hyperphosphorylation of NS5A, transactivates interleukin 8 promotor, suppresses HCV translation and modulates the ER stress response. Mutation in NS4B encoding gene may be targeted to develop an effective vaccine against HCV before virus invasion in host immune system.
Serum was collected from 20 patients who are infected with HCV. RNA was isolated from these samples to reverse-transcribe it into cDNA. This cDNA were PCR-amplified and amplicons were detected by UV light after their separation through agrose gel electrophoresis followed by seguencing of NS4B encoding gene.
Variations in HCV NS4B gene was analyzed by using Mega 6 software and sequence 2 blast analysis NCBI tool. Protein modeling was performed with phyre 2, DIANNA tool and I-Tasser online server and quality of model and antigenicity was checked by Vaxajen V 2.0 and Epijen softwares. Conservative analysis and epitope mapping were performed by using Immune Epitope Database (IEDB).
A systematic approach was employed for the prediction of potential epitopes in NS4B protein. Vaxijen V 2.0 was used to determine overall antigenicity of NS4B using cut off value of 0.4. Values above this threshold level show probable antigens in the protein. The topology of NS4B was determined by TMHMM server with the help of membrane topology data. Regions outside membrane and transmembrane were eradicated for epitopes prediction. T cell epitopes propred was used with proteosome cleavage site filter of 4% threshold. From the following data analysis 12 to 15 possible T-cell epitopes can be predicted; only these T cell epitopes were included in conservation analysis.
The present study provides information about mutational changes in HCV protein NS4B and thus preliminary data for the development of vaccine against HCV. This can also lead to future prospects for diagnosis, treatment and prognosis of HCV.
Availability: Items available for loan: UVAS Library [Call number: 2339-T] (1).
