1.
Effect Of Two Synchronization Protocols (Ovsynch Va 2Pg)And Gnrh Pn Day Of Breeding On Reproductive Performancein
by Hasan Riaz | Prof. Dr. Nasim Ahmad | Dr. Mian Abdul Sattar | Prof. Dr.
Material type: ; Format:
print
Publisher: 2011Dissertation note: The main objective of the present study is to compare the two synchronization protocols (Ovsynch vs 2PG) on estrus response, follicular development, ovulation, pregnancy rate and fecundity in Beetal and Teddy breeds of Pakistan. The secondary objective is to determine the efficacy of GnRH at the time of breeding on pregnancy rate in Beetal goats. Experiment 1 was conducted with a total of 28 goats during breeding season (September-October) at Livestock Production Research Institute, Bahadurnagar, Okara. The goats were randomly allocated in one of two treatment groups, Ovsynch and two PGF treatments. The Ovsynch treated does received an intramuscular injection of GnRH analogue 12.5µg lecirelin (Dalmarelin®, Fatro, Italy), on "Day 0". On "Day 7" does were treated with injection of PGF2? analogue 37.5µg d-cloprostenol (Dalmazin®, Fatro, Italy,) followed by a second injection of 12.5µg lecirelin (Dalmarelin®, Fatro, Italy), after a 48h period. Animals in the double PGF group received two injections of 37.5µg d-cloprostenol (Dalmazin®, Fatro, Italy), 10 days apart. In order to determine the estrous response and its duration, two aproned bucks were used twice daily for 30 minutes after the last PGF2? injection in both groups until the termination of estrous signs. All goats were naturally bred 12h after the onset of estrus. Follicular and luteal activity was monitored by transrectal Ultrasonography (ALOKA, SSD 900 Japan) with the aid of a 7.5-MHz linear array transducer daily- from the start of treatment, until estrus and then twice a day until the cessation of large follicles (apparent ovulation). On day 7 after breeding, all goats were re-examined by transrectal ultrasonography to determine the ovulation rate, based upon the number of CL's present. Pregnancy rate and fecundity (no of embryos per doe) was again determined at Day 30 after breeding by transrectal ultrasonography. Experiment 2 was performed on 25 goats. These does were randomly allocated to two groups (GnRH, n=11 and Control, n=14). GnRH does received 12.5µg lecirelin on the day of natural breeding (12h after standing estrus), while the control does did not receive any treatment. Timing of ovulation, pregnancy rate and fecundity were assessed by transrectal ultrasonography as in Experiment 1. Estrus (Experiment 1) was detected in 10 out of 14 does (71%) in Ovsynch group, while all 14 goats (100%) of double PGF responded group. The interval from the last PGF injection to estrus was 48.0 ± 2.6h in the Ovsynch group, while 36.0 ± 1.2h in the double PGF group (P<0.05). The interval from the last PGF injection to ovulation did not differ significantly between the Ovsynch (73.0 ± 4.8h) and double PGF (66.0 ± 2.7h) groups respectively. Similarly the interval from standing estrus to ovulation was not significant between the Ovsynch (24.0 ± 3.7h) and double PGF (30.0 ± 2.7h) treatments respectively. Estrous duration was similar in both treatment groups (44.7 ± 4.9h for Ovsynch and 47.1 ± 2.9h for the 2PGF). The size of ovulatory follicle was similar in both treatment groups (Ovsynch, 7.1 ± 0.2mm; 2PGF, 7.1 ± 0.1mm). On day 7 after breeding, the ovulation rate (no. of CL's on the ovary) were similar in both the Ovsynch (1.6 ± 0.2) and double PGF (2.0 ± 0.2) groups. The pregnancy rate as determined by transrectal ultrasonography on day 30 (Fig. 1) revealed 6/10 (60%) in the Ovsynch and 11/14 (78%) in the double PGF groups respectively (not significant). Fecundity was similar for both treatment groups (1.6 ± 0.2). In Experiment 2 the interval from standing estrus to ovulation recorded was not significant (31.2 ± 2.9h and 36.0 ± 5.3h), the ovulatory diameter (6.8 ± 0.1mm, 7.2 ± 0.5 mm (P<0.05), the pregnancy rate (54% (6/11) and 64% (9/14), and fecundity (1.5 ± 0.5, 1.7 ± 0.5) were not significant between GnRH treated and control does respectively (Table 2). From these experiments it can be concluded that the Ovsynch protocol, which is a non-steroidal and safe approach for estrous synchronization appears to be similar to the double PGF injection protocol, in terms of the reproductive performance in goats. However, this needs to be tested on larger number of animals. Furthermore, the use of GnRH at the time of breeding does not improve the reproductive performance in goats. The data generated has important implications for commercial goat farming in the developing countries.
Availability: Items available for loan: UVAS Library [Call number: 1295,T] (1).
2.
Prevalence Of Brucellosis In Dairy Animals And Their Handlers In District Bannu, Khyber Pakhtoon Khwa
by Azmatullah Khan | Dr. Amjad Riaz | Dr. Hassan | Dr. Mian Abdul Sattar.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1641,T] (1).
3.
Effect Of Biostimulation During Estrus Synchronization Protocols On Pregnancy Rate In Buffalo
by Abdul Nasir | Dr. Amjad Riaz | Dr. Aqeel | Dr. Mian Abdul Sattar.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1666,T] (1).
4.
Effect of Timing of Artificial Insemination and Ovulation in Relation to Onset of Standing Heat on Pregnancy Rate in Nili-Ravi Buffaloes
by Umair Riaz | Prof. Dr. Nasim Ahmad | Dr. Mian Abdul Sattar | Prof. Dr.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2013Dissertation note: Artificial insemination (AI) is one of the first and most influential biotechnologies of animal agriculture. To be beneficial artificial insemination must be done properly. There are many factors involved in quality of AI performance, including insemination technique, technician, semen quality, cleanliness, etc. However, the single most important factor in achieving high conception rate in buffalo is the timing of AI relative to the timing of ovulation. Application of AI is difficult because of poor estrus expression by buffaloes and poor estrus detection by owners, a highly variable duration of estrus and the difficulty to predict time of ovulation. The objective of the present study was to determine the effect of A.I at different time intervals (0, 12, 24 hours after the onset of standing heat) in relation to time of ovulation on pregnancy rate in buffalo. For this purpose, buffaloes with average body condition 2.5 ± 0.5 at Livestock Experimental Station Bhuneki, Buffalo Research Institute, Pattoki District Kasur during breeding season (September to December) were exposed to teaser bull two times in a day at 12 hours interval (6:00 am in morning and 6:00 pm in evening) to determine the standing heat (0h). Buffaloes, allocated randomly, at different time intervals i.e., either 0, 12 or 24 hours after the onset of standing heat were bred artificially using frozen thawed semen. Trans-rectal ultrasonography (HS 1500V; Honda; Japan, with 7.5 MHz transducer) was used to determine ovarian follicular development and ovulation, from onset of standing heat till ovulation after every 12 hours. Pregnancy status was determined by ultrasonography, 30 to 40 days post AI. Results revealed that the overall time of ovulation from onset of standing heat averaged 35.33 ± 0.21 h. Pregnancy rates were 26% (8/30) at 0 h, 37% (10/27) at 12 h and 53% (15/28) at 24 h after the onset of standing heat. It is concluded that timing of ovulation is about 35 h and 24
maximum pregnancy rate are achieved when bred after 24 hours after the onset of heat in buffaloes.
Availability: Items available for loan: UVAS Library [Call number: 1694,T] (1).
5.
Effect Of Control Internal Drug Release & Human Chorionic Gonadotropin Administration On Day 7 After Fixed Time
by Ali Husnain | Prof. Dr. Nasim Ahmad | Dr Mian Abdul sattar | Prof. Dr.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1697,T] (1).
6.
Association Between Numbers Of Ovarian Follicles And Fertility In Nili-Ravi Buffaloes
by Qaisar Shahzad | Dr. Amjad Riaz | Dr. Jawad Nazir | Dr. Mian Abdul Sattar.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2013Dissertation note: 6.1: Back Ground:
The water buffalo (Bubalus bubalis) is an important animal of livestock species. It has an essential role in the economy of developing countries. Buffalo is being used as dairy, beef and draught purpose. Despite so many qualities, the reproductive potential of the buffalo is lower than cattle. One of the reasons for low reproductive potential is smaller ovaries and low number of ovarian follicles. Due to these reasons superovulation and embryo transfer has not been much successful in buffaloes. There is no tool through which reproductive performance of buffaloes can be phenotyped. Antral follicular count can be a tool on the basis of which reproductive performance of buffaloes can be phenotyped
6.2: Hypothesis:
Higher the antral follicular count higher will be the fertility in Nili-Ravi buffaloes.
6.3: Methodology:
Ten Nili-Ravi heifers were used in the study to measure repeatability of antral follicular. Each heifer was scanned on alternating days from day 1 of estrous cycles to day 9 of the successive estrous cycle. Antral follicles were counted in different follicular waves and repeatability of antral follicles was assessed. In the 2nd experiment of the study 10 heifers were used to count antral follicles from day 1 to day 10 of the estrous cycle, on the basis of antral follicular count animals were divided into three groups, Low (1-4), Intermediate (5-8) and High (?9).Blood sampling was done on 7th day of estrous cycle to measure progesterone concentration of 3 animals from each group and an association was developed between antral follicular count and progesterone concentration. In the 3rd experiment, 25 Nili-Ravi buffaloes were used to antral follicles from day 1 of estrous cycle to day 10 of estrous cycle. , on the basis of antral follicular count animals were divided into three groups, Low (1-4), Intermediate (5-8) and High (?9). In the next heat animals were artificially inseminated and were checked for pregnancy on 36th and 60th day by using ultrasound. Animals pregnant on 60th day were considered as pregnant. After that association was developed between antral follicular count and fertility.
6.4: Results:
Buffalo can be phenotyped on the basis of antral follicular count. Follicular counts are highly repeatable in different follicular waves of same estrous cycle (0.83) and different estrous cycles (0.85) within individual animals. Follicular counts are highly positively correlated (0.91) with progesterone concentration. Higher the antral follicular count, higher should be the progesterone concentration. Antral follicular count is highly positively associated with (0.99) with fertility. Higher the antral follicular count higher will be the fertility.
Availability: Items available for loan: UVAS Library [Call number: 1734,T] (1).
