1.
Comparayive Efficacy Of Chemotherapeutic Trails Against Hypodermosis In Cattle In Rajanpur
by Muhammad Zahid | Dr. Aneela Zameer Durrani | Dr. Muhammad Hassan Saleem | Prof. Dr. Azhar.
Material type: ; Format:
print
Publisher: 2011Dissertation note: Study was carried out in district Rajanpur from February, 2011 to April, 2011 to find out percentage of infestation and to study chemotherapeutic trails against warble fly infestation in cattle in three tehsils namely Rojhan, Rajanpur and Jampur of district Rajanpur. In each tehsil 200 cattle were examined to record clinical signs of hypodermosis. For treatment of affected animals drug trail were done with Trichlorophon (Tagafon Star,Pakistan) and Ivermectin (Imec, SJG, Pakistan) 1% subcutaneously @ 0.2mg/kg body weight. Study included percentage of infestation in cattle in the households, veterinary hospitals and private farms. Average percentage of warbles in cattle was calculated and found 10 %, 17% and 16.5% in Rojhan, Rajanpur and Jampur respectively. It was found that percentage of infestation was 12% to 18% in hilly areas as compared to semi-hilly areas with 8% to16% and riverine areas 8% to 18% warble fly infestation in cattle. It was observed that warble fly infestation in cattle was absent in some plane areas in tehsil Jampur.
The comparison of different chemotherapeutic trails against Hypodermosis in cattle were observed and recorded. Three groups of cattle were made for chemotherapeutic trails against hypodermosis. Application of Tagafon in two different groups of cattle was carried out to evaluate their efficacy against Hypodemosis. In one group Trichlorophon (Tagafon Star,Pakistan) was applied with cotton gauzes by swabbing and spraying method with 5% solution of trichlorophon. In second group Trichlorophon (Tagafon Star, Pakistan) was given by drenching method with 2% solution at a dose rate of 0.2ml/kg body weight. In third group (Inj.Imec, SJG, Pakistan) Ivermectin 1% at dose rate of 0.2mg/kg body weight subcutaneous injection was carried out. Ivermectin1% was found to be 100% effective. Tagafon (Trichlorophon) was found 90% effective by swabbing and spraying method with 5% solution and Tagafon (Trichlorophon) by drenching method with 2% solution was found 85%effective against warble fly infestation in cattle in the study area. The data was analyzed statistically and found significant.
Availability: Items available for loan: UVAS Library [Call number: 1297,T] (1).
2.
Chemotheraph Of Ancylostomiasis In Cats In Lahore.
by Awais Bokhari | Prof. Dr. Muhammad Sarwar Khan | Dr. Muhammad Hassan Saleem | Dr. Nisar.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2012Dissertation note: It has been a long time since the affection of cats is with human beings. Cats have recorded as most adoptable pet of the modern world. Cats can be found anywhere in the world. Many diseases of cats are zoonotically important and they may cause zoonotic problem in human and mainly these pathogen are involved in enteric problems. The relationship of feline with human does provide many benefits to them but also act as a cause of zoonotic diseases.
A total of 300 fecal samples were collected from cats and were examined in the Clinical Medicine Laboratory, University of Veterinary and Animal Sciences, Lahore. The positive samples for Ancylostoma were 65 out of 300, indicating prevalence of Ancylostomiasis was 21.66%. For the chemotherapeutical trails 18 positive cats and 6 healthy cats were divided into four groups viz. A, B, C and D with 6 animals in each group. The cats of group A were treated with Pyrantel Pamoate @ 20mg/kg orally. The cats of Group B were treated with the Albendazole @ 50mg/kg orally. The cats of Group C were kept as positive control while the cats of Group D were kept as negative control.
The fecal samples were collected on day 0 pre-treatment and then on day 7th, 14th and 21st of post-treatment. The efficacy of the drugs was calculated on the basis of reduction of the eggs count from the fecal samples on the respective days. The efficacy of Pyrantel Pamoate on these days was 43.47%, 67.39% and 94.20% respectively, against Ancylostoma. The efficacy of Albendazole on these days was 30.77%, 60.44% and 86.81% respectively, against Ancylostoma. Thus it was recorded
that Pyrantel Pamoate was more effective against Ancylostoma than Albendazole as it caused more reduction of the egg count than albendazole.
Hematological study showed that the values of hemoglobin, packed cell volume and total erythrocyte count were lower in groups A, B and C as compared to non-infected control group. The values of total leukocyte count were higher in group A, B and C as compared to non-infected group. As a result of treatment in group A and B, the values of hemoglobin, packed cell volume and total erythrocyte gradually increased while that of total leukocyte count gradually decrease on day 7th, 14th and 21st post-medication. It has been observed during the present study that Pyrantel Pamoate and Albendazole showed their positive effect on blood parameters as a result of Ancylostomiasis with more efficacy of Pyrantel pamoate as compared to that of Albendazole.
Availability: Items available for loan: UVAS Library [Call number: 1391,T] (1).
3.
Prevalence And Chemotherapy Of Gastro-Intestinal Paradites In Deer In Punjab, Pakistan.
by Ruheeb Aslam | Dr. Jawaria Ali Khan | Dr. Muhammad Hassan Saleem | Dr. Nisar.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2012Dissertation note: The parasitic load on deer affects them badly and eventually leads to death. In the present study a total of 100 feacal samples were collected from deer and were examined in the Clinical Medicine Laboratory, department of CMS, University of Veterinary and Animal Sciences, Lahore. Out of 100 samples thirty eight were positive and among these thirty eight, 12 deer were infected with cestodes, 6 with nematodes, 12 with trematodes, and 8 with the mixed infection of parasites. So, the prevalence of the cestodes, trematodes, nematode and mixed infection was 12%, 12%, 6% and 8% respectively. For the chemotherapeutic trails thirty positive animals and 10 healthy animals were divided into four groups A, B, C and D with 10 animals in each. The animals of group A was treated with albendazole @ 7.5mg/kg orally. Those of group B was treated with the ivermectin @ 1mg/ 50kg orally. The group C animals were kept as positive control and group D were kept as negative control.
The feacal samples were collected on the 0 day (pre-treatment), 7th, 14th and 21st day (post-treatment). The efficacy of the drugs were calculated on the basis of reduction of the eggs count from the feacal samples on the respective days. The efficacy of albendazole 42.59 %, 71.3% and 87.96% against cestodes, 33.33%, 60.3% and 82.88% against nematodes and 40.18%, 68.22% and 89.72% against trematodes at day 7, 14 and 21 respectively.. The efficacies of ivermectin were 42.59 %, 73.15% and 88.88% against cestodes, 51.78%, 76% and 91.07% against nematode while 42.85%, 64.28% and 85.71% against trematodes at day 7, 14 and 21 respectively.
It is thus concluded that the ivermectin is more effective drug than albendazole as it caused more reduction of the egg count than albendazole.
Availability: Items available for loan: UVAS Library [Call number: 1392,T] (1).
4.
Prevalence And Chemotherapy Of Theileriosis In Equines In And Around Lahore
by Muhammad Azhar | Dr. Muhammad Ijaz | Dr. Muhammad Hassan Saleem | Prof. Dr. Azhar.
Material type: ; Format:
print
Publisher: 2012Dissertation note: Theileriosis is an infectious malady of equidae characterized by intermittent fever, loss of appetite, anemia, loss of weight, dyspnea, pale mucous membranes, jaundice and recumbency. Considering the significance and utilization of equines in our country and the substantial losses rendered by theileriosis, the present study was designed to study prevalence and chemotherapy of theileriosis in equines in and around Lahore.
For this purpose 300 equines (Horses=100; mules=100; donkeys=100) were examined for theileria infection.The blood was collected by ear tip puncture and from Jugular venipuncture. Then thin blood smear slide was prepared and dried up in air and stained with Geimsa's staining method and examined under microscope. Theileriaequiwere identified by their morphological characteristics as described by Rashid et al. (2009) and Guimaraeset al. (1997).Over all prevalence of Theileriaequiin equines was estimated as 58%. There was also a mixed infection of Theileriaequi and Babesiacaballiin some cases. Individually there was 54%, 64% and 56% prevalence of Theileriaequi found in horses, mules and donkeys respectively.
The effect of theileriosis on various blood parameters (Hb, ESR, TEC, TLC, DLC and PCV) was determined in 30 equines (Horses= 10; mules= 10; donkeys= 10). The remarkable decrease in hemoglobin, TEC, PCV and lymphocytes was observed. The value of ESR increased while there were no remarkable changes in TLC, monocytes, basophils, neutrophils and eosinophil.
For chemotherapy, 36 equines (Horses=12; mules=12; donkeys=12) positive for theileriosiswere divided into three groups (A, B, and C). Each group was further subdivided into three subgroups comprising of 4 animals in each subgroup. The animals of group A (Horses=4; mules=4; donkeys=4) were treated with Imidocarbdipropionate (Imizol®, ICI, Pakistan) @ 2 mg/kg BW I/M, and the efficacy of drug was found 91.7% in equines against theileriosis.The group B (Horses=4; mules=4; donkeys=4) was treated with Buparvaquone (Butalex®, ICI, Pakistan) @ 2 mg/kg BW I/M alone and was observed 66.7% effective. While the group C (Horses=4; mules=4; donkeys=4) was treated with Auk(Calotropisprocera)@ 0.3mg/kg BW, 8 doses at alternate days (local treatment), which was found 58.3% effective. The efficacy of drugs was measured on the basis of disappearance of clinical signs and blood smear examination at day 2, 4, 6 and 10 of post-medication. The efficacy of each drug was measured through recovery rate of the animals.
Finally, the data on prevalence of theileriosis was estimated by Pearson's chi-square test for significance whereas data on hematology was analyzed by Student's T-test using statistical software package STATA 9.1 (College Station T×77845, USA), P < 0.05 was considered significant.
Considering the significance and utilization of equine in our country and the substantial losses rendered by theileriosis, the present project was designed to check prevalence and chemotherapy of theileriosis in equines in and around Lahore. The results of this study will help farmers and veterinary practitioners in field.
Availability: Items available for loan: UVAS Library [Call number: 1487,T] (1).
5.
Srudy Of Mycotoxicosis In Cattle And Buffalo In District Sheikhupura, Punjab.
by Salman Arshad | Dr. Aneela Zameer Durrani | Dr. Muhammad Hassan Saleem | Ms. Sehrish.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1618,T] (1).
6.
Prevalence Of Dermatophytosis In Domestic Dogs And In-Vivo Evaluation Of Topical Anri-Fungal Agents
by Muhammad Mudassar Aziz | Dr. Asim Khalid Mahmood | Dr. khalid Saeed | Dr. Muhammad Hassan Saleem.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2011Dissertation note: The pet dog is a domesticated animal, belonging to the family Canidae and it comes within the order Carnivora. The given nomenclature is used for all varieties of pet dogs. Earlier, this pet animal was used for different purposes which include hunting, fighting and also different types of work had been taken from it. Dermatophytosis is the common skin infection in dogs especially in the young ones. A total of 300 samples were collected. The 36 samples were found positive and then divided into 3 groups. In group K, Ketoconazole was applied topically twice a week. Later on the efficacy of Ketoconazole was observed by collecting the samples on day 7, 14 and 21 after the commencement of treatment, which was 20%, 60% and 90% respectively. Similarly, Group L was treated with Lime sulphur and the efficacy of Limesulphur against M.canis infection, was found to be 60%, 80% and 100% on day 7, 14 and 21 respectively. Group M was treated with Miconazole. The efficacy of miconazole against M.canis was found to be 50%, 75% and 100% on day 7, 14 and 21 respectively. At the end, it was concluded that Lime sulphur was the best drug of choice among the three drugs used and the efficacy of ketoconazole was lesser than the others.
Availability: Items available for loan: UVAS Library [Call number: 1304,T] (1).
7.
Effects Of Aflatoxin M1 (Afm1) On Sheep And Efficacy Of Mycotoxin Binders
by Muhammad Akhtar (2008-VA-122) | Dr. Jawaria Ali Khan | Dr. Muhammad Hassan Saleem | Dr. Muhammad Ovais Omer.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: Livestock production is an important part of national economy and it plays a significant role in providing the high quality food for human beings. Dairy industry of Pakistan contributes up to 46.8% in the agriculture and about 10.8% of the GDP in the form of milk, milk products, meat, hides, skin and bone meal (Ghaffar et al. 2007). Molds are fungi consisting of filaments that are present in the feed of animals. Molds can cause many infections in dairy cattle, especially during period of stress when their immune system is depressed. They cause a special disorder that is termed as Mycosis. Specific mycotoxins are produced by these fungi and when these animals eat such type of feed then they also engulf such dangerous mycotoxins. Mycotoxins are produced by a wide range of various molds and can be classified as secondary metabolites meaning that their function is not essential to the mold’s existence (Whitlow and Hagler 2005).
In the European countries, sheep milk is mainly produced in the Spain, and it is about 403000 tons per year. The main purpose of this milk is to produce cheese. Manchego cheese is considered as the best quality cheese in the world. This is the only technique by which we can produce the high quality of cheese (Rubio et al. 2009). These Aflatoxins are the fungal main dangerous constituents that can contaminate the feed of animals very easily. If AFB1 contaminated ration is consumed by animals then metabolism takes place in these toxins and excrete AFM1 in milk which further reduces milk production. More long-lasting aflatoxin poisoning produces very dissimilar signs that may not be clinically superficial and decreased rate of production in young animals. Severe aflatoxicosis causes hepatitis, hemorrhage, and death. Decreased growth rate is the most delicate clinical symbol of chronic aflatoxicosis, and it may be the only readily obvious abnormality. Natural toxins considerably show the greater danger to human and animal health. One large group of natural toxins that are universally
Introduction
2
documented as poisons of food and feed are the mycotoxins. The toxins occur naturally in various animal feeds, including corn and cottonseed. To control AFM1 in milk, it is essential to decrease the feed contamination by AFB1 (Prandini et al. 2009).
The livestock is playing major role in enhancing agricultural productivity and it has also major contribution to decrease the rate of poverty in the rural areas of Pakistan. Most farmers have domesticated sheep in their home which are their major sources of income (Mahmood et al. 2009). Aflatoxins adulterated rations prompted important reduction in daily feed consumption and the means of body weight, body weight increment and feed transformation rates were radically affected during the exposure stage to aflatoxins. Furthermore, serum ingredients and ruminal measurements showed lessened liver function and digestive turbulences in sheep fed aflatoxin (Jouany and Diaz 2005)
Aflatoxins are included in that group of toxins which are considered as highly toxic toxins. Aspergillus parasiticus, Aspergillus flavus and Aspergillus nomius are those types of fungi which can produce very strong type of toxins. Aflatoxin B1 (AFB1) is considered as the most dangerous type of aflatoxin and it is very strong hepato-toxin (damaging the liver as a whole). It can also cause cancer, can produce teratogens, and may also cause mutation in the both animals and in human beings.
AFB1 being very toxic, can be activated by the special metabolism of liver and through cytochrome P450. The cancer causing ability of AFBI is ten times more than the AFM1. The conversion of AFB1 to AFM1 in the milk producing animals is in the range of half percent to about six percent. In milk producing animals the level of aflatoxin M1 is greatly altered by the contamination rate of aflatoxin B1 (Bognanno et al. 2006).
It is observed that the excretion pattern of aflatoxin M1 in the milk of sheep is lower than the excretion pattern of aflatoxin M1 in the milk of cows. Due to presence of aflatoxin B1 in the feed of animals and hence appearance of aflatoxin M1 in the milk of animals shows that
Introduction
3
milk of such animals becomes more toxic and more un-hygienic. The young ones having milk as their main feed ingredient may have much more chances of getting various infections. (Var and Kabak 2009). To check the level of aflatoxin M1 in the milk of sheep, various experimental studies have been performed in the whole world (Battacone et al. 2005). In Pakistan, there are many favorable environmental conditions in which various types of fungi can easily prompted (Iqbal et al. 2011). The contamination level of aflatoxin M1 in the milk of dairy animals and in the dairy products has been found by (Hussain et al. 2008).
Aflatoxins can be produced from fungal metabolites. Aflatoxin M1 is produced as the complete metabolism of aflatoxin B1 has taken place. Aflatoxin B1, when converted into aflatoxin M1 is appeared in the milk of that animals and the same aflatoxin M1 can also be appeared in the dairy products which can contaminate the feed of human too. Transformation of aflatoxin B1 into the aflatoxin M1 is around one to two percent. This transformation is highly dependent upon the feed of animals, transformation from one affected animal to other healthy animal, transformation from the milking of affected animal to the milking of healthy animal and from one day to another day also. It is also noted that as the level of aflatoxin B1 in the feed of animal is decreased down then the level of M1 in the milk of animals is also dropped down significantly but it takes about 72 hours for this dropped down (Nilchian and Rahimi 2012).
AFM1 had a resistant to thermal inactivation used during food processing procedure such as pasteurization and autoclaving. Storage of various dairy products was not effective in the reduction of this toxin (Ozdemir 2007). Aflatoxin M1 level in the milk of animals can also be found in 12 to 24 hours after the ingestion of aflatoxin B1. After this, its level can be raised within the few days. The estimation of conversion rate of aflatoxin B1 in the contaminated feed of animals into aflatoxin M1 in the milk that animal is elaborated as 1 to 3 percent in a specific range (Ozdemir 2007).
Introduction
4
Aflatoxin B 1 being very much dangerous mycotoxin, it is very much important to know about such methods by which the level of aflatoxin B1 in the feed of animals can be controlled. For this purpose we should first come to know the basic source of feed contamination of animals, and after this we should adapt such easy method to determine the level of aflatoxin B1 in the animal’s feed and such methods should be very cost effective. The best accepted and recognized method to determine aflatoxin M1 level in the milk of infected animals is HPLC high-performance liquid chromatography and TLC thin-layer chromatography (Thirumala-Devi et al. 2002). Aflatoxin M1 can be found in the milk of infected animals and can also be found in the products that are prepared from that contaminated milk. This contamination is the main problem arising now a days in the whole world (Fallah et al. 2009). Availability: Items available for loan: UVAS Library [Call number: 2237-T] (1).
8.
A Study On The Correlation Of Serum Electrolytes And Trace Elements Along With Associated Risk Factors In Diarrheic Foals
by Rahmatullah (2008-VA-85) | Dr. Muhammad Ijaz | Dr. Muhammad Hassan Saleem | Prof. Dr. Kamran Ashraf.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: Foal diarrhea is one of the major threats to equine industry that causes high morbidity and mortality. Electrolytes (Na, K, Ca, Fe, Cu and Li etc) and water losses reduced dietary intake and probable translocation between intracellular and extra cellular compartments. Electrolytes are essential for controlling membrane potential, muscle contraction, nerve condition, and enzyme reactions, and they play a central role in the physiological processes. Serum electrolytes and trace elements deficits are the major causes of fluid and acid-base imbalance in diarrheic foals. Keeping in view the importance of foal diarrhea and its impact on the equine industry the current study was designed to determine serum concentration of electrolytes (Ca, K, Na) and trace elements (Li, Cu, Fe) along with the associated risk factors in diarrheic foals.
The present study was designed to examine diarrhea in foals at various equine stud farms, private and public veterinary hospitals. For this purpose a total of (n=100 foals) suffering from clinical diarrhea irrespective of the cause of diarrhea selected randomly after clinical examination and (n=10 foals) negative for diarrhea as control were included. Data of each diarrheic foal was collected separately in a data capture form. Blood and serum sample were collected for hematological and serum biochemical studies, respectively. Concentrations of serum electrolytes (Ca, Na, and K) were analyzed by Flame Photometry and trace elements (Cu, Li, and Fe) were estimated through Atomic Absorption spectrophotometer. PCV was determined by micro hematocrit method. Risk factors including species and age were analyzed for association.
Foals which were suffered from diarrhea showed increase in Pack cell volume. Serum concentration of Sodium, potassium, calcium, iron, copper and lithium were measured in diarrheic foals by atomic absorption and flame photometer method and were compared with healthy group of foals.Foals having diarrhea showed an acid baseimbalance as a result decrease in serum electrolytes and trace elements. The blood acid-base balance is precisely controlled because even a minor deviation from the normal range can severely affect many organs. The body uses different mechanisms to control the blood's acid-base balance.Sodium and potassium and calcium are important electrolytes imbalance in diarrhea. Potassium level was increased in foals having diarrhea. Calcium concentration in ppm significantly decreased from its normal value. In some diarrheic foals increased in level of calcium was also observed because these foals have blood in their feces that result in the calcium to come in blood as a clotting factor. Trace elements such as Cu, Fe and Li are needed in a very little concentration for many physiological functions. The serum iron was measured in foals having diarrhea.The iron concentration was less than normal value.The deficiency of iron lead to anemia. Clinical signs of anemia because of irondeficiency are rarely seen in foals. Therefore, mineral imbalances influence iron metabolism and may cause iron deficiency in foals. The serum copper concentration was significantly decreased in the diarrheic foals. Copper concentration is also related to the packed cell volume as in anemia copper deficiency is a significant feature. Copper is important element and its deficiency lead to decrease in serum concentration of iron. This decreased level might be because most of the circulating copper in plasma is attached to the serum glycoprotein, ceruloplasmin. Ceruloplasmin has ferroxidase activity and may be required to deliver iron into the circulation Therefore it was observed that low iron concentrations might have resulted from copper deficiency.The concentration of Lithium was also decreased in diarrheic foals when compared to healthy foals.
Availability: Items available for loan: UVAS Library [Call number: 2349-T] (1).
9.
Experimental Treatment Of Bovine Brucellosis Using Phyto-Chemo-Immuno Theraputic Agents
by Muhammad Kaleem (2013-VA-860) | Prof. Dr. Aneela Zameer Durrani | Dr. Ihtisham Khan | Dr. Muhammad Hassan Saleem | Prof. Dr. Khushi Muhammad.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: Bovine brucellosis is very important zoonotic disease in respect of outbreak that resulted in reproductive loses through storm of abortions in herds and infertility in bulls. Currently, no phyto treatment for complete recovery from brucellosis is available.
Phyto Chemo-immune therapeutic agents are effective for recovery from bovine brucellosis (Hypothesis)
Group A was comprise five adult cows (n=5) positive for brucellosis. Animals in this group were given 3 shots of Oxytetracycline LA at 20mg/kg BW (IM) repeated every 48 hrs. Streptomycin @ 13mg/kg BW IM was given for six consecutive days. Furthermore, flunixin meglumine @ 2mg/kg BW IM for 3 days in combination with antibiotic. Selevit injection containing selenium and vitamin E was administered (IM) for consecutive five days. At next day all the animals in this group were vaccinated with a combined vaccine of RB51 and FMD (Oil based) at a dose of 3mL/animal through deep IM route. In group B, all the animals were given Saafi (Herbal Product) orally consecutively for six days. Furthermore, flunixin meglumin @ 2 mg/kg BW IM was administered for three days. All these treatments were given simultaneously. Then injection Selevit containing selenium and vitamin E were administered (IM) for consecutive five days. After treatment all animals were vaccinated with RB51 + FMD (oil based) 3mL/ animals deep intramuscularly. In group C, flunixin meglumin @ 2 mg/kg BW IM was administered for three days. After NSAID, Selevit injection containing vit. E and selenium was administered (IM) for five days regularly. After treatment, animals were vaccinated with RB51 + FMD (oil based) 3 mL/ animals (IM). In D group five negative adult animals were included in this group and vaccinated subcutaneously with RB51 and FMD (Oil based) at a dose of 3 mL/animal.
Summary
40
Data regarding this study was analyzed with ANOVA and completely randomized design was used to compare the mean between different groups using statistical package for social science (SPSS) version 20. P < 0.05 was considered significant.
The results showed OD values after 1st, 3rd and 6th month were significantly different in all groups. The mean values depicted that OD values was significantly higher (2.886±0.099) in vaccinated (positive) animal’s vs the other three groups. There was no significant difference in OD values of herbal treated, antibiotic treated and health control animals. The lowest OD values was found in healthy control animals, though significantly similar with herbal treated, antibiotic treated. The mean OD value at 3rd month after treatment is significantly higher (1.794±0.090) in herbal treated and vaccinated positive groups but the OD value of healthy group was lowest (1.794±0.090) after 3rd month of treatment. After 6th month of treatment the OD value of positive vaccinated group was found significantly highest (1.146±0.194). On the other hand lowest value (0.595±0.079) was found in healthy control group.
All the groups have found non-significant difference in all group. Regarding Real time PCR the value range from 0-40 Ct values. In the present study the Ct values of the control positive was found to be 13 which indicate control positive. The antibiotic treated group had a Ct value found in this range 35.61 to 38.13. The herbal treated group were all positive and the values were 31.34 to 37.42 Ct. The vaccinated immune booster group. The Ct values in the group was found to be 30.83 to 34.21. The birth weight and placenta dropping time was normal in antibiotic treated animals. There were no significant results found in all groups.
Summary
41
This study concluded that herbal regimen is effective in vitro against brucellosis but in vivo it is yet not to be evaluated. The antibiotics can be effective to treat the Brucellosis with this protocol. This was help to control bovine brucellosis, and extensive economic losses. Comparative efficacy of four commonly-immune therapeutic agents were help in choosing the most effective therapy/method for the recovery of bovine brucellosis. Availability: Items available for loan: UVAS Library [Call number: 2388-T] (1).
10.
In-Vivo Efficacy Of Aqueous And Alcoholic Extracts Of Hedera Helix Against Fasciolosis In Sheep
by Muhammad Faisal Raza (2008-VA-206) | Dr. Muhammad Avais | Dr. Muhammad Hassan Saleem | Dr. Imran Rashid.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: The liver fluke, Fasciola hepatica, is a common parasite of ruminants in many countries in the temperate climates and often causes severe economic losses. Infection with both F. hepatica and F. gigantica may also occur in occasionally causing large scale epidemics. These parasites adversely affect the health status of animals and cause enormous economic losses Triclabendazole (TCBZ) is a worldwide, one of the most used drugs for the control of fasciolosis but the efficacy may depend on the susceptibility of the strain involved in the drug. However, resistance of the liver fluke to TCBZ in naturally infected sheep has been reported. Plants are being used as anthelmintic as the allopathic drugs today which offer substitute to the use of synthetic chemicals. This study was carried out to evaluate the efficacy of aqueous and methanolic extracts of H. helix against fasciolosis in sheep. For this purpose, adult sheep was used for In vivo trials. Animals were screened for the presence of liver fluke and 30 sheep positive for liver fluke infestation were used for therapeutic trials. They were divided into 5 groups. Group A and B were given single dose of aqueous extract of Hedera helix at concentrations of 1.13 gm/kg and 2.25 gm/kg body weight respectively. Animals in C and D group were given 1.13 gm/kg and 2.25 gm/kg body weight respectively, while sheep in E group were given Triclabendazole at dose rate of 1 ml/5kg body weight. Fecal samples from each animal was collected in the morning, starting from day 0 pre-treatment and at days 4,7 and 14 post-treatment and were evaluated for the EPG through Modified McMaster method.
The comparative efficacy was analysed by two way analysis of variance (ANOVA) using SPSS 20.0. A probability levels ˂0.05 was considered as statistically significant.
During In vivo experiment all the treatments showed significant reduction in EPG. The maximum reduction in EPG showed by crude aqueous extract (CAE) at day 4 post treatment at 1.13 gm/kg dose rate was 20.0012 %. There were no significant reduction in EPG at day 7 and 14 day for aqueous extract. The maximum reduction by aqueous extract at day 4 at dose
Summary
29
rate of 2.25 gm/kg was 40.47 % and at day 7 it was 45.24 %, whereas there was no significant reduction at day 14 post treatment. The maximum reduction showed by crude Methanolic extract at dose rate of 1.13 gm/kg at day 4 was 29.54 %. At day 7 post treatment the reduction of EPG was 40.09 % and at day 14 the reduction was 43.18 %. The maximum reduction showed by crude Methanolic extract at dose rate of 2.25 gm/kg at day 4 was 56.09 %. At day 7 post treatment the reduction of EPG was 64.85 % and at day 14 the reduction was 68.29 %. The positive control treated with Triclabendazole showed the maximum reduction of 97.5 % in eggs per gram (EPG).
So it is concluded from the study that the extracts of Hedera helix have the anthelmintic efficacy against liver fluke. The increase in dose rate of extracts may give better results. So there is need to conduct further studied and more trials on Hedera helix extracts under field conditions to achieve maximum efficiency levels. Availability: Items available for loan: UVAS Library [Call number: 2431-T] (1).
11.
Comparative Efficacy of Steroids and Non Steroids Anti-Inflammatory Drugs On Wound Healing In Equines
by Sajid Ali (2009-VA-129) | Prof. Dr. Muhammad Arif Khan | Dr. Hamid Akbar | Dr. Muhammad Hassan Saleem.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Blank CD. Availability: Items available for loan: UVAS Library [Call number: 2502-T] (1).
12.
Effect Of Cholesterol Loaded Cyclodextrin (Clc) Addition On Post Thaw Quality Of Jack Semen
by Muhammad Rafi Ullah (2009-VA-54) | Dr. Mushtaq Ahmad | Dr. Muhammad Zahid Tahir | Dr. Muhammad Hassan Saleem.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Improvement of post-thaw quality of Donkey (Equusasinus) semen is essential to augment the in-vivo and in-vitro fertilization rate. Whenever we think about the techniques for long term storage of the germ cells cryopreservation appears most useful but it causes lethal and sub-lethal damage to the sperm. Sperm cryosurvival rates are not optimal for most species including donkey because of its plasma membrane composition. Egg yolk is an important component of most of the equine freezing extenders. But another factor regarding this important component is that its higher concentrations can have some deleterious effects such as oxidase activity of dead spermatozoa, bacterial/ xenobiotic contamination, reduced respiration and motility of the spermatozoa, which ultimately results in lowering fertility rate. That’s why, cold shock resistance is more in those species semen possessing higher membrane cholesterol to phospholipids ratio as compare to those species semen having less cholesterol to phospholipids ratio. Indeed it may be a useful strategy to improve cryopreservation protocols for jack sperm. Addition of CLC may also be a substitute for egg yolk in semen extender always considering that the cryo-protective effect of EY is partially due to its high cholesterol content. Semen was collected in artificial vagina at 42ºC from two mature donkeys (n=2) five times (replicates=10) which were maintained at Ravi campus Pattoki. Semen samples possessing >60% motility were used in this study. Each ejaculate was divided into 6 aliquots and CLC was added into these aliquots according to desired concentrations (0 mg, 1.5 mg and 3 mg/120 million sperms) then kept at 25ºC for 15 minutes. After incubation semen aliquots were diluted in 1:1with centrifugation media and centrifuged at 600g for 15 minutes to separate seminal plasma after bead formation. Beads of the semen were resuspended in the centrifugation media having different concentrations of egg yolk for different CLC concentration groups in such a way that 100 million / ml sperms concentration was achieved. When this final dilution was done semen was shifted at 4 ºC for 2 hour
CHAPTER 6
SUMMARY
Summary
37
cooling then further 2 hours for equilibration at 4º C. Then semen was packed in 0.5 ml plastic straws. These straws were suspended on liquid nitrogen vapors upto7 mints then plunging these straws in the liquid nitrogen in freezing box and then shifting these straws to the liquid nitrogen container by placing them in the goblets and stored there until post thawing was done. After post thawing semen was analyzed for motility, Acrosomal and plasma membrane integrity, live ratios DNA integrity and MDA levels. Post-thaw parameters were analyzed by PROC MIXED as factorial ANOVA using SAS enterprise Guide Version 4.2. And it was observed that. And it was observed that both CLC 1.5 and 3 mg/120 million sperms with full egg yolk did not improved (p˃0.05) the post thaw quality except in malondialdehyde levels in which 3 mg dose with full yolk significantly (p˂0.05) decreased the level of malondialdehyde. While CLC with lower levels of egg yolk maintained the quality same as the control or improved significantly in some parameters.CLC1.5 HEY and CLC3 NEY both compete the control in motility, PMI by HOST and total viability parameter..DNA integrity increased with the decrement of egg yolk as CLC1.5 HEY, CLC3 HEY and CLC3 NEY are significantly (p˂0.05) better than the remaining three groups.MDA level is significantly lower in CLC1.5 HEY and CLC3 HEY groups comparable to control means that with partial removal of egg yolk and supplementation of CLC malondialdehyde levels are decreased significantly as compare to control. Thus CLC substitutes the egg yolk completely when 3mg of CLC /120 million sperms is added in kenney’s extender for jack semen cryopreservation. Availability: Items available for loan: UVAS Library [Call number: 2553-T] (1).
13.
Comparative Efficacy Of Albendazole, Pyrantel Pamoate, Ajwain And Kamala Against Toxocara Vitulorum Infestation In Bovine Calves
by Muhammad Zahid IQbal (2007-VA-72) | Prof. Dr. Aneela Zameer Durrani | Dr. Muhammad Hassan Saleem | Dr. Arshad Javid.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Toxocara vitulorum is a round worm of cattle and buffalo that is common in tropical and subtropical area.Toxocara vitulorum from infected cattle and buffalo transmitted to calves via colostrum and placenta while its transmission was very less though feed and water. Toxocara vitulorum infestation was very high in calves and it caused mortalities in calve ages between 1 to 3 months, while infestation was less in high age groups.Mortality in cattle and buffalo calves reached up to 50% andcause poor growth, colic, constipation, diarrhea, anorexia and ketosis in calves. These worms could cause intestinal strangulation, holes and blockage in intestines of calves.
Resent study was designed to check the prevalence and therapeutic trial of Toxocara vitulorum in cow and buffalo calves. These results were very helpful for the treatment of the Toxocara vitulorum that was a major endo-parasite in the cows and buffalo calves. Fecal samples from 300 cows and buffalo were examined under the compound microscope for ova. Positive cow and buffalo calves were divided in five groups and different treatments were given to each group. Egg per Gram (EPG) counted at day 7and 14th post-treatment.
Overall prevalence Toxocara vitulorum was 49% in cow calves and 59% in buffalo calves. Prevalence was higher in 1-3 month age group calves (78% in cow calves & 91% in buffalo calves) while prevalence was higher in female calves (52% in cow and 61% in buffalo calves) as compare to male calves (44% in cow and 55% in buffalo calves). Prevalence was higher in the summer stress months.
The efficacy of the Albendazole was lowest in both cows and buffalo calves. The efficacy of Albendazole in cow was 25% and 31% at day 7th and 14th, respectively while in buffalo calves the efficacy of Albendazole was 24% and 31% at 7th and 14th days of post treatment, respectively. The efficacy of Pyrantel pamoate was 98% and 100 % in cow calves at day 7th and 14th, respectively while in buffalo calves the efficacy of Pyrantel pamoate was 81% and 100% at day 7th and 14th, respectively. The efficacy of Ajwain in cow calves was 59% and 69% at day 7th and 14th, respectively while in buffalo calves it was 58% and 69% at day 7th and 14th, respectively. The efficacy of Kamala in cow calves was 33% and 39% at day 7th and 14th day of post-treatment, respectively and buffalo calves the efficacy was 34% and 42% at day 7th and 14th of post-treatment.
It is concluded from the present study that both in cow and buffalo calves, Toxocara vitulorum is most prevalent parasitic infestation. This parasite is more prevalent in female calves, 1-6 months of age and during hot and humid season in both cows and buffaloes. Pyrantel pamoate is proved to be better than Ajwain but Albendazole and Kamala was not justified good dewormer against Toxocariasis in bovine calves.
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Availability: Items available for loan: UVAS Library [Call number: 2656-T] (1).
14.
Clinico Epidemiology of tick Borne Hemoparasitic Diseases Using Single Round And Multiplex PCR Along With their Phylogenetic Analysis In Bovine
by Shahid Hussain Farooqi (2012-VA-447) | Dr. Muhammad Ijaz | Dr. Muhammad Hassan saleem.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: CD Crupt Availability: Items available for loan: UVAS Library [Call number: 2760-T] (1).
15.
Clinico Epidemiology of tick Borne Hemoparasitic Diseases Using Single Round And Multiplex PCR Along With their Phylogenetic Analysis In Bovine
by Shahid Hussain Farooqi (2012-VA-447) | Dr. Muhammad Ijaz | Dr. Muhammad Hassan saleem | Dr. Muhammad Imran Rashid.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: CD Crupt Availability: No items available
16.
Prevalence And Treatment Of Helminths In Race Horses During Summer Season In Lahore
by Syeda Qurrat-Ul-Ainn Zanjani (2008-VA-162) | Dr. Waseem Yaqub | Dr. Muhammad Hassan Saleem | Dr. Nasir Ahmad.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Horses and humans interact in many ways including a wide variety of sports competition, police work, agriculture, entertainment and warfare. The transport of horses across national boundaries for shows and performance competitions make the issue of worm control and containment of resistant strains a matter of global importance.
Among the diseases affecting horses, helminths infections are much extended. These parasites continue to be a significant threat to the health of horses. The nature or extent of damage varies with the parasites. They cause loss of nutrients, blood and serious economic losses. The control of nematodes in horses has been challenging for some years and there is general recognition that improvements should be made to commonly used worm control programs. These improvements should be based on scientific knowledge of the parasites, appropriate diagnostic techniques and should take into account the presence or potential selection for resistance to available anthelmintic. Horses of all ages are affected without sex or breed susceptibility. There is, therefore, a need to understand the current status of the prevalence and intensity of infection of helminths in horses
The prevalence and intensity of infection of helminths is influenced by the origin, age, and sex of animals. Horse breeders should regularly contact veterinarians for prophylactic and curative treatment of their animals and also, pasture management should be strictly respected. So, keeping in view the importance and economic losses rendered due to parasitic diseases.The background of this study is to diagnose the prevalence of helminths in race horses through fecal examination techniques.
The study was carried out at the Lahore Race Club for 6 months. A total of 300 horses were examined randomly.Fecal samples were collected from sampled horses and processed by Direct microscopic examination, Salt floatation, McMaster egg counting techniques. The data regarding age of animals, clinical history, feeding, housing and various treatment protocols as well as feedback were entered in the questionnaire. 45 horses were found infected with various parasites with the overall prevalence of 13.33%. Out of 45 horses, 10 horseswere infected with strongylus. 2 with trichostrongylus, 8 with oxyuris, 14 with parascaris, 6 with strongyloides and 5 with mixed infection. So, the prevalence for strongylus, trichostrongylus, oxyuris, parascaris, strongyloides and mixed infection was 3.33%, 0.66%, 2.66%, 4.66%, 2.0% and 1.66% respectively.
Drug trials were carried on positive animals. 5 grams of fecal sample from each animal was collected and drug trials were carried into 4 groups, i.e. A, B, C and D. Each group comprises of 10 animals,Pyrantelpamoate at 19mg/kg B.W. once orally,Ivermectin at 2mg/kg B.W. orally as single dose,Oxfendazoleat 10mg/kg B.W. once, Neem leaves orally at 375mg/kg B.W. once, were used in these four groups. The feces were examined on day 3, 7, 11 and 14 post-medication. The efficacy of the drugs was calculated on the basis of reductions in the number of ova per gram of feces.
The efficacy of pyrantelpamoate was 53.32%, 53.08%, 51.0% and 50.52% on days 3,7,11 and 14 respectively. The efficacy of ivermectin was 71.67%, 93.32% ,97.56% and 98.78% on days 3, 7, 11 and 14 respectively. The efficacy of oxfendazole was61.5%, 69.06%, 72.47%, and74.72% on days 3, 7, 11 and 14 respectively. The efficacy of neem leaves was 41.37%, 40.32%, 38.76% and 38.74% on days 3, 7, 11 and 14 respectively. It was concluded that ivermectin was most effective.
In hematological examination hemoglobin estimation, packed cell volume, total erythrocyte count and differential leukocytic count was performed followed by the collection of 5ml of the blood aseptically. Groups A and D had low hemoglobin values. The values get improved in groups B and C after 4days, consecutive treatment of positive horses, the sample was drawn days from the relative groups to evaluate the effectiveness of treatment for lowering the EPG.
This study helped to control helminthic load. Comparative efficacy of the drugs was also help in choosing the most effective drug against these parasites.
Availability: Items available for loan: UVAS Library [Call number: 2775-T] (1).
17.
Qualitative And Quantitative Assessment Of Fertile Period In Bitches
by Anam Mushtaq (2009-VA-388) | Dr. Muhammad Zahid Tahir | Dr. Amjad Riaz | Dr. Muhammad Hassan Saleem.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: The estrous cycle of the bitch consists of 4 phases including proestrus, estrus, diestrus and an obligatory anestrus. Most of the owners normally assess the fertile period on the base of qualitative parameters and mate their bitches on predetermined days. This is the major cause of apparent infertility in bitches. The most reliable assessment for timing of mating is through quantitative assessment through vaginal cytology and progesterone assay. The objective of this study was to compare the outcomes of qualitative and quantitative assessment of fertile period in bitches. Furthermore, the effect of assessment method was studied on fertility (number of pregnant bitches) and prolificity (number of pups in a litter).
This study was conducted on 148 referral cases of bitches brought to Theriogenology Laboratory. The data of qualitative assessment by the owners was collected by a questioner while quantitative assessment was based on vaginal cytology and progesterone assay. Statistical analysis was done using SPSS and logistic regression to compare the fertility (number of pregnancies) and prolificacy (number of pups) among groups.
In qualitative group, only 32% bitches were diagnosed pregnant. In contrast, the quantitative group showed a significantly higher fertility (83%) as compared to qualitative group (P<0.05) with odd ratio of 3 times higher fertility. Similarly, in qualitative group, 58% bitches had litter of 1-4 pups while the rest had a litter ranging in 5-10 pups. In contrast, quantitative group showed significantly (P<0.05) higher prolificity with 79% bitches having a litter of 5-10 with 4 times higher odd ratio. Meanwhile, the percentage of bitches having a smaller litter (1-4 pups) was considerably low (21%). In conclusion, the quantitative assessment of fertile period is more accurate and results in significantly higher fertility and prolificity. Availability: Items available for loan: UVAS Library [Call number: 2946-T] (1).
