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1. Pereentage Of Hemorrhagic Septieemia Carrier Buffalo And Their Immunity Status At District Swat, Khyber

by Ikramul Haq | Dr. Aneela Zameer Durrani | Dr. Ayesha | Dr. Jaweria Ali Khan.

Material type: book Book; Format: print Publisher: 2012Dissertation note: The study was conducted in District Swat, Khyber Pukhtoonkhwa province, to study the percentage of carrier buffaloes of hemorrhagic septicemia in the region and Immune status of buffaloes. Tracheal swabs and blood were collected from 200 buffaloes slaughtered at Saidu Sharif slaughter house. The selected buffaloes were divided into four age groups, the first group had animals of two and less than two years of age, the second had animals with age of three years, the third group, five years and fourth group had animals with age of more than five years. Tracheal swabs were cultured for the isolation of Pasteurella multocida, for identification of carrier animals and blood sample were processed to determine the antibodies titer of buffaloes against Pasteurella multocida. The results showed that 8 (4%) out of total 200 examined buffaloes were identified as carriers of hemorrhagic septicemia by isolating the Pasteurella multocida from their tracheal swab. Out of 8 carrier buffaloes 4 were from group IV, 3 from Group III and 3 from group II. The percentage of carrier buffaloes in four age groups was 0%, 2%, 6% and 8% in group-I, group-II, group-III and group-IV respectively, showed that percentage of carrier buffaloes were high in group IV while lowest in group I. The Pathogenicity tests of the all 8 isolates showed that they were pathogenic to mice and cause their death within 24 hours of incubation. Serological study of blood sample for antibodies titer showed that out of 200 buffaloes 156 (78%) showed antibodies titer ?1:16 which were considered immune against hemorrhagic septicemia. Out of 156, 17 were from group-I, 46 from group-II, 46 group-III and 47 from group-IV. Percentage of immune buffaloes in different age groups was 34%, 92%, 92% and 94% in group-I, group-II, group-III and group-IV respectively. The higher number of immune animals i:e 94% were in group-IV and the lowest were in group-I i:e 34%. Results showed that all the carrier buffaloes had antibodies titer more than 1:16. The antibodies titer of the 4 carrier buffaloes were 64, 2 had 128 while 1, 1 had antibodies titer 16 and 32 respectively. Availability: Items available for loan: UVAS Library [Call number: 1443,T] (1).

2. Prevalence And Therapeutic Trials On Ticks And Mites In Sheep In And Around Lahore City

by Hafsa Maqsood Sana | Dr. Jawaria Ali Khan | Dr. Ayesha | Dr. Syed Saleem Ahmed.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2012Dissertation note: Sheep and goats sustain an expensive profitable and environmental position in Asian agriculture (Devendra 1996). Majority of the livestock owners earn their income through sale of additional animals and their by-products. There are several factors affecting the production potential of livestock. Among these, parasitic infestations are prevalent, affecting different livestock species all over the world. Parasitic infestation especially ecto-parasites are the major veterinary problems in most of the developing and under developed countries of the world. The physical condition and production in animals is severely affected by tick and mite infestation. It is one of the most common problem of sheep characterized by anemia, poor physical condition, decreased milk quality and poor meat production.. The present study was conducted on ticks and mites to study their prevalence, their effect on various blood parameters and to evaluate the efficacy of Ivermectin and Azadirchta indica (neem oil) on sheep in and around Lahore, Pakistan. In this study 300 sheep suspected for tick and mite infestation on the basis of clinical signs were collected from outdoor hospital of University of Veterinary and Animal Sciences Lahore, Outreach hospital Sitar wala and Civil Veterinary Hospital Thokar Niaz Biag. The tick sample were collected with the help of foreceps and mites sample through skin scraping. Out of this 300, 198 samples were collected from outdoor hospital UVAS Lahore, from which 84 were positive with prevalence of 42.42% for tick and mite infestation, and 62 samples were collected from outreach hospital Sitarwala from which 24 were positive giving prevalence of 38.70% and 40 samples were collected from CVH Thokar Niaz Biag, out of which 17 samples were positive with prevalence of 42.5%. All the collected samples were processed at medicine laboratory, UVAS, Lhr. Whereas in the third part therapeutic trials on 20 sheep (n=15 tick and mite infested and n=5 healthy sheep) was done and divided into four groups A, B, C&D. Each group was comprised of 5 sheep .In group A, the infested sheep were treated with two doses of ivermectin 0.2mg/kgS/C 7 days apart, on 7th day its efficacy was 40%and on 14th day it gave 100% efficacy. In group B the infested sheep were treated with neem oil (Azadirachta indica) @1 table spoon, its efficacy was 20% on 7th day and on 14th its gave 40% efficacy, in group C infested sheep were kept as positive control, whereas healthy sheep were included in group D. In the second part of present project haematological studies on 10 sheep (n=5 tick and mite infested sheep, n=5 healthy sheep) were performed to see the effect of tick and mite infestation on various blood parameters (Hb, PCV,TLC) and it was fsound that Hb and PCV values were decreased whereas TLC was increased in infested sheep. The therapeutic trials of this study concluded that the Ivermectin was most effective drug against ticks and mites in sheep. Availability: Items available for loan: UVAS Library [Call number: 1492,T] (1).



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