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Molecular Basis Of Antibiotic Resistanc In E. Coli Isolates From Poultry Drinking Water

by Hira Naseer | Ms. Sehrish Firyal | Prof. Dr. Masroor Ellahi Babar.

Material type: book Book; Format: print ; Literary form: drama Publisher: 2012Dissertation note: Back Ground: E. coli is a single-celled organism belonging to the large bacterial family Enterobacteriaceae, the enteric bacteria. Most of the E. coli strains are harmless but there are some serotypes of it that are pathogenic and cause serious food poisoning in human and major economic losses in both chicken and turkeys. Poultry, the second largest industry in Pakistan is supplied by lots of antibiotics like streptomycin, sulfamethoxazole and tetracycline, streptomycin and ampicillin etc, either through feed or water. Use of antibiotics at large scale is resulting in the development of antibiotic resistance in poultry and human. Hypothesis: To check the molecular basis of antibiotic resistance in E.coli and to find out the genotypic and phenotypic correlation between resistant E.coli from poultry drinking water. Methodology/Parameters: In this study, drinking water samples from poultry water were collected and cultured on MacConkey agar. Standard disk diffusion method will be used to check antibiotic susceptibility. Plasmid DNA was extracted from colonies showing antibiotic resistance by mini-prep protocol. Using universal set of primers, antibiotic resistant genes was amplified and then sequenced. The sequence thus obtained was compared in the database with previously reported sequences of antibiotic resistant gene in E. coli strains. Statistical Design: Prevalence of tetA and tetB genes was shown by a Column chart. Outcomes: This study helped to find out prevalence of these antibiotic resistance gene in E. coli isolated from poultry drinking water, which are potential threats to human being. Availability: Items available for loan: UVAS Library [Call number: 1497,T] (1).

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Genetic Characterization Of Pakistani Lathy Pigeons Using D-Loop, Cyt B And 16S Rrna Genes As Genetic Marker

by Muhammad Umair Latif | Ms. Sehrish Firyal | Dr. Ali Raza Awan | Dr. Muhammad.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1399,T] (1).

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Comparasion Of Differnt Presumptive Tests For Detection Of Bloodstain After Washing Fabric With Different

by Samreen Mushtaq | Ms. Sehrish Firyal | Dr. Muhammad Imran | Ms. Faiza.

Material type: book Book; Format: print ; Literary form: drama Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1674,T] (1).

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Level Of Amylase From Human Saliva Deposited On Fruit First Bite Mark

by Umar Draz | Ms. Sehrish Firyal | Dr. Mohammad Ashraf Tahir | Prof. Dr. Tahir.

Material type: book Book; Format: print ; Literary form: drama Publisher: 2013Dissertation note: Saliva is colorless fluid which consists of epithelial cells, enzymes, non enzyme protein and inorganic components. Saliva is secreted by three glands in mouth. One is parotid gland, second is submandibular gland and third is sublingual gland. There are two types of amylases in human. One is salivary amylase, while other is pancreatic amylase. The salivary amylase is secreted by salivary gland while pancreatic amylase is secreted by pancreas. The salivary amylase is present in saliva, perspiration and breast milk. Pancreatic amylase is present in blood, feces and urine. Saliva stain is very important at crime scene for forensic investigation. Majority of techniques used for detection of saliva are based upon the presence of salivary amylase. Human saliva can serve for identification. One can extract DNA from saliva stain and generate DNA profile, whereby individual can be identified who is a source DNA profile that is generated from saliva stain. In present study level of salivary amylase was determined from human saliva deposited on fruit with first bite mark. Apple, peach and apricot were selected for this experiment. Ten males and ten females were selected to bite on fruits. The time interval was used as variable for determining the level of amylase. The time intervals were 0 hour, 12 hours, 24 hours, 36 hours and 48 hours. Samples were collected from bite mark area on fruit. The samples collected from apples and apricot pits were positive for amylase activity till 48 hours. The samples collected from peach were positive till 12 hours. The samples collected from peach were negative after 24 hours. This research indicates that salivary DNA could be found on bite mark area on apple and apricot pit till 48 hours. Availability: Items available for loan: UVAS Library [Call number: 1755,T] (1).

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