000			01869nam a2200193Ia 4500
005			20150917130819.0
008			150525s1994 xx 000 0 und d
041			_aeng
082			_a0383,T
100			_aAmir Saeed, M _95397
110			_cProf. Dr. Muhammad Aleem Bhatti _95398
245			_aAntiseperim Antibodies: A Plausible Cause Of Repeat Breeding In Nili-Ravi Buffaloes
260			_c1994
502			_aThe present study was designed with the assumption that high titres of antisperm antibodies in repeat breeders might be a contributing factor for conception failure in such animals. For this purpose, 32 buffaloes/buffalo heifers were divided into four equal groups. From the normal cyclic and repeat breeder buffaloes, 48 serum samples were collected at 0, 1, and 24 hours after insemination, respectively. Oestrus mucus was also collected from these animals. A total of 16 serum samples were collected from virgin heifers and pregnant buffaloes. The serum and mucus samples were subjected to simple agglutination test, sperm quantitative penetration test and sperm cervical mucus contact test. High antibody titres were recorded in the serum and oestrus mucus of repeat breeders. Moreover, it was observed that lesser number of spermatozoa penetrated through a definite distance in cervical mucus of repeat breeder buffaloes as compared to cervical mucus of normal cyclic animals. Similarly higher number of spermatozoa exhibited shaking movement over a period of time in cervical mucus of repeat breeders, which indicates the presence of spermagglutinins. Thus, it can be concluded that antisperm antibodies may be responsible for conception failure in repeat breeder buffaloes.
650			_aDepartment of Theriogenology _916092
700			_aProf. Dr. Rashid Ahmad Chaudhary _95400
710			_aFaculty of Veterinary Sciences _94292
942			_cTH
999			_c2140 _d2140