000			01686nam a2200205Ia 4500
005			20150917144110.0
008			150525s1997 xx 000 0 und d
041			_aeng
082			_a0490,T
100			_aJameel Ahmad _95649
110			_cDr. Khalid Pervaiz _95585
245		2	_aA Serological And Coprological Study Of Paramphistomiasis In Buffalo
260			_c1997
502			_aTwo hundred faecal samples, serum samples and paramphistomes used in the present study collected from main slaughter house of Lahore and from surrounding of the city. Faecal examinations were carried out with fresh smear technique and 61.5% animals found positive for paramphistomiasis. Serum was separated from the blood samples collected from infected and suspected buffaloes for the performance of enzyme-linked immunosorbent assay. Paramphistome crude antigen was prepared from fresh stomach flukes, which contained 95.66 mg proteins/mi at 545 nm. For performing the enzyme linked immunosorbent assay, the 96 well ELISA plates were coated with crude antigen & kept overnight & then the other procedure for the assay was followed, & the serum samples were tested with paramphistome crude antigen. In positive samples, the yellowish-brown colouration was formed. In faecal examination, 61.5% and in the ELISA 100% of the animal found positive. From the conclusion of the results if was found that ELISA (100%) has given more positive results then faecal examination (61.5%).
650			_aDepartment of Clinical Medicine & Surgery _94563
700			_aDr. Haji Ahmed _95483
700			_aDr. Muhammad Ashraf _94741
710			_aFaculty of Veterinary Sciences _94292
942			_cTH
999			_c2243 _d2243