000			02209nam a2200193Ia 4500
005			20150917144557.0
008			150525s1997 xx 000 0 und d
041			_aeng
082			_a0500,T
100			_aAkif Masood _95709
110			_cAtta-ur-Rehman Rizvi _95710
245			_aPhysicochemical Factors Effecting The Survival Of Egg Drop Syndrome Virus
260			_c1997
502			_aAbout 417 duck eggs were collected from a duck farm. These eggs were incubated at 37oC in automatic incubator for 10 days. At the 11th day the eggs were candled to confirm the fertility of eggs, either they are embryonated or not. Fertile eggs were then be inoculated with the physically and chemically treated EDS-76 virus which have already been treated and stored in plastic vials at -20oC. About 0.1 ml of the sample was inoculated per egg. Four eggs were set for each of the factor i.e physical and chemical. The physical factors were temperature, pH and U.V light. The current project was conducted to study the survival of EDS virus when it was subject to various physical and chemical factor. As far as the physical factors were concerned it was observed that at different temperatures i,e -2oC, +4C°, 331::0 and 37C°. The virus survived at each temperature far 35 days and the same virus survived at 56C° for 90 minutes. AS far as the pH was concerned it was examined that EDS virus remained viable at pH 1,47,10,13 for 24 hours. Following exposure of virus to WY light it was observed that EDS virus servived for 45 minutes. Similairly, the results of chemical factors showed that formalin of 0.067. could not inactivate the virus but 0.12% and o.24x formalin solution killed the virus in time from 6 to 24 hours. Losan with 0.5%., 1.0%. and 1.5%. killed the EDS virus in 15, 30 and 45 minutes respectively. The results of this endeavor show that the formalin and Losan in other words chemical like these should be one of the options for farmers to disinfect their sheds to prevent the occurrence of infection from EDS virus.
650			_aDepartment of Microbiology _94609
700			_aDr. Muhammed Shakeel akhtar Khan _95711
710			_aFaculty of Veterinary Sciences _94292
942			_cTH
999			_c2253 _d2253