000			02364nam a2200205Ia 4500
005			20150917154153.0
008			150525s1997 xx 000 0 und d
041			_aeng
082			_a0529,T
100			_aSajid Mahmood _95856
110			_cDr. Muhammad Akram Muneer _95562
245			_aStandardisation Of Indirect Haemagglutination Test For Monitoring Infectious Bursal Disease Virus
260			_c1997
502			_aIndirect haemagglutination (IHA) test was standardized and evaluated to moniter antibodies against infectious bursal disease (IBD). It was observed that oil based vaccine prepared from bursae of fäbricius of infected birds, induced a high level of antibody which were detected by agar gel precipitation test (AGPT). It was recorded Chat tannic acid, glutaraldehyde and chromium chloride had 0.0000781, 0.003906 and 0.0001562 per cent subagglutinating dilutions in normal saline solution (pH 7.2) respectively while 0.000001220, 0.0156 and 0.0025 subagglutinating dilution of the coupling agents were found in phasphate buffered saline (pH 7.2), respectively. Indirect. haemagglutination test is sensitive and specific serological technique to study infectious bursal disease. However, antigen dilution to sensitize erythrocytes, source of erythrocytes, chemical nature of diluent, interaction temperature and time, nature and concentration of coupling agent coated erythrocytes and antiserum against IBD, had influenced the sensitivity of IHA test. Ten percent antigen for sensitizing sheep erythrocytes, incubation temperature of 37°C for 10 minutes for antigen, tannic acid (0.005%) and erythroéyte interaction, freshly prepared sensitized erythrocytes and normal saline solution (pH 7.2) as diluent were found suitable for detecting maximum titre of anti-IBD antibodies through the IHA. Moreover it was observed that the standardized IHA proportionally showed reduction in the titre on dilution of serum. The antibody titre in the IHA was the well having serum dilution, showing resistance to bleed (flow) on tilting the plate for 5 seconds. The final results of antibody titre were achieved within 120 minutes post processing of the samples.
650			_aDepartment of Microbiology _94609
700			_aDr. Hajid _95385
700			_aDr. Khushi Muhammad _95480
710			_aFaculty of Veterinary Sciences _94292
942			_cTH
999			_c2277 _d2277