000			02374nam a2200205Ia 4500
005			20150917154512.0
008			150525s1997 xx 000 0 und d
041			_aeng
082			_a0531,T
100			_aAbid Akbar, M _95864
110			_cDr. Muhammad Naeem _95395
245			_aIn Vitro Studies On The Effects Of Physico Chemical Factors On The Survival Of Mycoplasma Capri
260			_c1997
502			_aThe physical factors under study were pH and temperature. It was observed that at p11 6.5, 7.5 and 8.5, although the growth of the organism took placed but the growth was best achieved at pH 6.5 with 30% absorbance measured by turbidometery. As far as the temperature is concerned, growth at different temperatures i.e. at room temperature (30°C), at incubator temperature (37°C), at refrigerator temperature (4°C) with respect to various sources of serum i.e. horse, cattle, buffalo, sheep and allontoic fluid, were tested and it was observed that at room temperature the growth was best in media containing buffalo serum 25% absorbance, respectively. At incubator temperature the growth of ycoplasnja pj was good enough with media containing cattle, buffalo and sheep serum, giving 25%, 32% and 33%, absorbance respectively and even at refrigerator temperatvye the growth of the said organism was available with media containing cattle serum, buffalo serum, sheep serum and allontoic fluid, giving 9%, 12%, 11.5% and 9% absorbance respectively. The results of chemical factors showed that the survival and growth of Mycoplasma capri was best attained at these serum sources i.e. horse, cattle and buffalo giving 28%, 26% and 29.5% absorbance, respectively. The effect of different concentrtions of horse serum i.e. 5%, 15% and 20% on ithe growth of the organism depicted that 15% concentration level is good for its growth and survival, giving 31.5% absorbance. Various disinfectants i.e. Formaline, Phenol, Beloran-500 (Ciba), Detol (Reckitt & Colman) were tested for disinfection against the said organism revealed that 1% solution of Formaline, 1% solution of Phenol, 0.5% solution of Beloran-500 and 4% solution of Detol effectively killed the bacteria.
650			_aDepartment of Microbiology _94609
700			_aDr. Muhammad _94698
700			_aDr. Muhammad Amin Sheikh _95865
710			_aFaculty of Veterinary Sciences _94292
942			_cTH
999			_c2279 _d2279