000			02671nam a2200205Ia 4500
005			20150921152452.0
008			150525s 2004 xx 000 0 und d
041			_aeng
082			_a0885,T
100			_aIqbal Munir _97199
110			_cProf. Dr. Ijaz Ahmed _97200
245			_aEffect Of Sperm Storage Tubules Secretions From Pre-Layer Hen On Cockerel Sperm Clumping And Motility
260			_c2004
502			_aArtificial insemination (AT) in the poultry industry has considerable importance because of better results in fertility and hatchability. Increasing male utilization in artificial insemipation depends upon the optimum use of semen by suitable diluting media to increase the volume of ejaculate and to preserve fertility. In the present study, the effect of sperm storage tubules secretions on percentage motility and extent of clumping of sperms was noticed. An optimum osmotic pressure 375 mOsm with pH 7.0 was used to preserve the cockerel's semen at 5°C. A total of 20 meat Breeder cockerels were randomly selected. After providing 10-days sexual rest, they were trained for semen collection by abdominal massage technique. Three birds failed to produce good quality semen. These birds were removed from the study. Semen from seventeen Meat Breeder Cockerels was pooled. After macroscopic evaluation, the pooled semen was divided into 4 groups.Group A was diluted with Modified Van Wambeke diluent with addition of sperm storage tubules secretions. Group B was diluted with the above diluent without SST secretions. Group C was diluted with saline solution with addition of SST secretions while group D was diluted with saline solution without SST secretions. These four groups were stabilized at 375 mOsm osmotic pressure in pH 7.0 and stored at 5°C. The diluted semen samples were examined for percentage motility and extent of clumping. After 72 hours of semen storage (5°C), group A showed significantly (P<0.05) higher motility as compared to groups B, C and D. The extent of clumping was higher (P <0.05) in group D as compared to groups A, B and C. However, group A showed less (P <0.05) clumping upto 64 hours as compared to groups B, C and D. The results of the present study suggested that at 375 mOsm, pH 7.0 the cockerels semen stored at 5°C diluted wiih Milk based extender and saline solution with addition to SST secretions proved to be suitable for short-term preservation of Meat Breeder Cockerel semen.
650			_aDepartment of Theriogenology _916218
700			_aDr. Amir Saeed _97202
700			_aProf. Dr. Zafar Iqbal Chaudhry _97203
710			_aFaculty of Veterinary Sciences _94292
942			_cTH
999			_c2613 _d2613