000			02123nam a2200205Ia 4500
005			20150921154127.0
008			150525s2005 xx 000 0 und d
041			_aeng
082			_a0910,T
100			_aSyed Amer Mahmood _97276
110			_cProf. Dr. Ijaz Ahmed _97200
245			_aEffect Of Cold Shock On Frozen-Thawed Spermatozoa Of Buffalo & Cow Bulls
260			_c2005
502			_a Effect of cold-shock on frozen-thawed bovine spermatozoa was measured in terms of motility, viability, plasma membrane and acrosome . integrity. Single ejaculates each from seven Nili-Ravi buffalo bulls and 7 Sahiwal cow bulls were processed for freezing. Three straws of 0.5ml (for each parameter) from each bull were thawed and pooled at 3rC before and after cold-shock at 4°C for 2 min. Individual motility was observed by phase contrast microscope before and after cold-shock. Before and after cold-shock, sperm viability, plasma membrane and acrosome integrity were evaluated by supravital stain, hypo osmotic swelling test and normal acrosomal reaction, respectively. Two hundred sperm were counted for each parameter before and after cold-shock. Comparison of before and after cold shock values showed that cold shock had no effect (P>0.05) on motility (64.0±1.88 vs 60.4±2.56), viability (141.1±4.39 vs 124.5±8.03), plasma membrane integrity (83.51±5.82 vs 71.19±4.31) and acrosome integrity (148.3±2.36 vs 141.1 ±2.85) of buffalo bull spermatozoa. Comparison of before and after cold shock values of cow bull spermatozoa indicated that cold shock had significant (P<0.05) effect on motility (59.2±1.05 vs 41.9±.12), viability (140: 14±2.94 vs 90.5±2.73), and plasma membrane integrity (62.3±4.28 vs 47.24±3.71) but no effect on acrosome integrity (147.9±2.21 vs 140.6±2.40). In conclusion, cold shock had significant detrimental effect on cattle bull spermatozoa but no effect on buffalo bull spermatozoa.
650			_aDepartment of Theriogenology _916219
700			_aDr.Azhar _95913
700			_aDr.Muhammed Amir Saeed _97278
710			_aFaculty of Veterinary Sciences _94292
942			_cTH
999			_c2636 _d2636