000			01880nam a2200193Ia 4500
005			20151008141559.0
008			150525s2007 xx 000 0 und d
041			_aeng
082			_a0984,T
100			_aMubashir Hussain _97792
110			_cProf. Dr. Muhammad Akram Muneer _96232
245			_aFactors Affecting Hemagglutination Potential Of Avain Influenza Viuruses (H5, H7, H9 Subtypes)
260			_c2007
502			_aThe objective of this study was to standardize hemagglutination and hemagglutination inhibition tests for AIV H5, H7 and H9 subtypes. These subtypes were propagated in 09-day old chicken embryonated eggs and after 72 hours post incubation the allantoic fluid (AF) was harvested and confirmed by spot agglutination test and by AGPT. While standardizing HA test maximum titers were recorded using 1% RBCs of chicken, human blood group Qe and dog using phosphate buffer saline (PBS) as a diluting agent for washing suspension of erythrocyte and by incubating the micro titer plates at 22c or 37C for 30 minutes or 40 minutes time period. The AIV subtypes eluted rapidly with increase in temperature with maximum elution observed within the time period of 8 hours. The live AIV provided much higher HA titer when compared with the titers obtained from AJV subtypes inactivated with formalin or Binary ethylene imine (BET). The BET was found to have little effect on HA activity as compared to formalin. While standardizing the HI test the best titers were obtained using 4 HA units of AIV antigen as compared to 1 HA and 8 HA units of antigen and by incubating the micro titer plates for 60 minutes period (time given for antigen-antibody reaction before the addition of erythrocytes suspension).
650			_aDepartment of Microbiology _94609
700			_aDr. Mansur-ud-Din Ahmad _95677
710			_aFaculty of Veterinary Sciences _94292
942			_cTH
999			_c2707 _d2707