000			01980nam a2200193Ia 4500
005			20151005133326.0
008			150525s2011 xx 000 0 und d
041			_aeng
082			_a1274,T
100			_aMuhammad Yasir _97472
110			_cProf. Dr. Kamran Ashraf _95070
245			_aComparative Efficiency Of Routine Identification Methods With Molecular Technique (Pcr) For Detection Of Caecal Eimeria Species in Broilers
260			_c2011
502			_aCaecl coccidiosis is caused by Eimeria tenella and E. necatrix and poultry industry is facing huge economic losses due to this infection world wide. Eimeria tenella is the most common cause of caecal coccidiosis but E. necatrix is also isolated rarely. In the present study, 400 caecal samples suspected for coccidiosis were collected from two districts (Lahore and Gujranwala). Ten samples from twenty farms of each district were collected and examined for coccidiosis and species identication. Prevalence of coccidiosis was found 68%. It was more prevalent in district Gujranwala (71.5%) as compared to Lahore (64.5%). Eimeria species were identified by conventional (Direct Microscopy, Sedimentation technique, Floatation Technique and Sporulation) and molecular technique (PCR). Polymerase chain reaction was found the most sensitive ands accurate technique for species identification as compared to all conventional techniques. The prevalence percentage of E.tenella and E,necatrix was found 68% and 12.5% respectively. E.tenella was more prevalent in district Gujranwala (71.5%) as compared to district Lahore (64.5%). E.necatrix was also more prevalent in district Gujranwala (13.5%) than district Lahore (11.5%). The difference in percent prevalence of coccidiosis and species of Eimeria may be due to difference in farm management, farming type, biosecurity measures.
650			_aDepartment of Parasitology _94290
700			_aDr. Aftab _96259
700			_aProf. Dr. Azhar Maqbool _95188
942			_cTH
999			_c3005 _d3005