000			02215nam a2200193Ia 4500
005			20151005151022.0
008			150525s2011 xx 000 0 und d
041			_aeng
082			_a1373,T
100			_aSadia Munir _97927
110			_cDr. Asif Nadeem _95126
245			_aSingle Nucleotide Polymorphisms In Pouifi Gene And Its Association With Milk Production Traits In Pakistani Cattle
260			_c2011
502			_aIn farm animals, the primary focus of selection has been to improve milk yield. Milk field is a polygenic trait and a few potential candidate genes have been recognized. Association studies have shown that POUlFl is related to many production traits in domestic animals and is one of those candidate genes that are involved in milk production. POUl F 1 encodes a pituitary-specific transcription factor. It is well established that growth hormone (GH) released from pituitary gland plays an essential role in growth, mammary gland development and lactation process. The bovine POUlFl gene is of 15952 bp length having 6 exons. The genetic characterization of the POUlFl gene to identify the SNPs as genetic markers and validation of these potential markers by associating them with milk production traits has been performed. A total 35 samples from Sahiwal and 30 from Holstein-Friesian cattle breeds were sequenced for all 6 exonic portions of the POUlFl by using 6 sets of primers. A total 15 polymorphic sites in Sahiwal and 14 in Holstein-Friesian were identified from these sequences. Out of total 15 SNPs identified in Sahiwal, 12 were in intronic region and 3 were in exonic. Out of 14 SNPs identified in Holstein-Friesian, 10 were in intronic and 4 were in exonic region. The sequences of the amplified POUl F 1 gene fragments were aligned with the help of BLAST for SNPs identification. This study is first step in finding some confirmed markers for milk yield in Sahiwal and Holstein- Friesian cattle breed that can be used in future for selection and breeding programmes.
650			_aInstitute of Biochemistry & Biotechnology _95022
700			_aDr. Abu Saeed _95180
700			_aDr. Muhammad Wasim _95181
942			_cTH
999			_c3086 _d3086