000			02062nam a2200181Ia 4500
005			20151006131202.0
008			150525s2012xx 000 0 und d
041			_aeng
082			_a1495,T
100			_aMuhammad Asim Raza _98142
110			_cDr. Arfan Ahmad _98143
245			_aIn Process Quality Control Factors Affecting The Potency Of Indigenous Mycoplasma Gallisepticum (Mg)
260			_c2012
502			_aMycoplasma gallisepticum (MG) is cause of chronic respiratory disease (CRD) and is responsible for significant economic losses to poultry industry. In Pakistan, imported MG bacterin fails to induce immunoprophylaxis that could be due to subtle antigenic variation in the immunogen of the vaccine. Therefore present study was conducted to optimize inactivations( phenol, formalin and binary ethylenimine) concentration and exposure time to inactivate MG and their effect on potency of MG bacterin ( prepared from local isolates) along with different bacterial biomasses (0.5%, 1% and 1.5% PCV) and adjuvants (montanide oil ,gel and water ). It was observed that the MG bacterin containing 1.5% level of immunogen/biomass induced significantly higher anti-MG-ELISA antibody titer (p < 0.05) as compared to other bacterins containing lower concentrations of the immunogen.The formaldehyde inactivated the pathogen within shortest possible time and showed undetectable effect on its potency. The antibody response was significantly higher (p<0.05) as compared to that of bacterins prepared from the pathogens inactivated by either phenol or BEI. . Montanide ISA70 containing MG bacterin induced significantly higher anti-MG-ELISA antibody titer (p<0.05) in broilers than the other bacterins containing either water or aluminum hydroxide gel. It is concluded that formaldehyde inactivated oil based vaccine containing one percent immunogen (0ne percent PCV) induce antibody response in broilers that is comparable with the imported vaccine.
650			_aDepartment of Microbiology _94609
700			_aProf. Dr. Masood Rabbani _96052
942			_cTH
999			_c3199 _d3199