000			02194nam a2200193Ia 4500
005			20151006131357.0
008			150525s2012 xx 000 0 und d
041			_aeng
082			_a1501,T
100			_aShahzad Asghar _98149
110			_cProf. Dr. Muhammad Ashraf _94840
245			_aEffect Of Vitis Vinifera (Grapes) Fruit Juice On Angiogenesis.
260			_c2012
502			_aAngiogenesis, the novel blood vessels formation, is a mark of nearly every neoplastic and non-neoplastic degenerative disease. Thetreatment of angiogenesis with plant derived anti-angiogenic substances may be proved as more efficacious,harmless, and lowbudget therapies. Grapes contain many vital natural compounds which can significantly prevent diseases and maintain human health. In this work, we have studied the effect of grapes juice (Vitis vinifera) on angiogenesis by use of chicken chorioallantoic membrane (CAM) assay. We took Fresh fertilized eggs and sprayed them with 70% ethanol for decontamination and incubated at 37?C and (humidity 65%-70%). At 5th day of incubation small windows were made on all eggs, 4-5 ml of albumin was extracted, windows were sealed with sterile Para-film tape and eggs were reincubated. Grapes juice was obtained from fully ripened grapes and various dilutions were formulated by using distilled water. Dilutions of the juice were filtered by special syringe filters used for experimental analysis and applied to the CAMs on 6th day of incubation. Changes in vessels were clearly observed macroscopically among all treated CAMs on 7th day of incubation. The total diameter & area of primary, secondary and tertiary blood vessels was observed to be reduced after treatment with grapes juice in a concentrationand dose related manner. Scanning probe image processor(SPIP)software was used for 3D surface roughness measurements which clearly demonstrated the antiangiogenic effect of grapes juice on CAM. Grapes (Vitis vinifera) juice inhibits angiogenesis, which is a common denominator of various major diseases.
650			_aDepartment of Pharmaoclogy & Toxicology _95037
700			_aDr. Muhammad Ovais Omer _95891
700			_aProf. Dr. _94896
942			_cTH
999			_c3205 _d3205