000			01878nam a2200193Ia 4500
005			20151006131843.0
008			150525s2011 xx 000 0 und d
041			_aeng
082			_a1507,T
100			_aMuzna Rana _98160
110			_cDr. Asif Nadeem _95126
245			_aVariation In The Non Structural Nd3 Gene Of Hepatitis C Viruses Isolated From Local Hospital Samples
260			_c2011
502			_aHepatic C virus (HCV) infects approximately 4% of the population worldwide. HCV establishes a chronic infection of the liver that causes cirrhosis and can lead to the development of hepatocellular carcinoma. HCV is transmitted exclusively through direct blood-to-blood contacts between humans. Current drug therapies for HCV infection often fail to resolve viral infection; therefore, new approaches for treating HCV infection are crucial in controlling chronic HCV infection. The viral protease NS3 has been identified as an attractive target for anti-HCV drugs, because its activity is indispensable for processing many of the NS proteins of HCV. Although the NS3 helicase is a potentially attractive target for anti-HCV drugs, no helicase inhibitors have yet entered clinical trials. In this research we are going to isolate the NS3 region of HCV genome, from different HCV positive patients with the help of RT PCR. Then sequence these, and observe similarities in these sequences. The main objective of the present study is to determine variation in the non structural gene NS3 of hepatitis C viruses isolated from patients suffering from hepatitis and reporting to local hospitals. The ultimate objective would be to utilize this data generated in this study attempting to prepare vaccine against this virus.
650			_aInstitute of Biochemistry & Biotechnology _95022
700			_aDr. Abu Saeed _95180
700			_aMrs. Saeeda Awais _98161
942			_cTH
999			_c3211 _d3211