000 02628nam a2200181Ia 4500
005 20151006132805.0
008 150525s2012 xx 000 0 und d
041 _aeng
082 _a1534,T
100 _aHaider Noor
_98196
110 _cProf. Dr. Mansur-ud-Din Ahmad
_95767
245 _aDetection Of Carrier And Subclinical Infection Of Babesia Ovis Trough Pcr At Government Farms Of Punjab
260 _c2012
502 _aHaemoprotozoan infections in ruminants are of significant importance in tropical and subtropical regions of the world. Diseases are transmitted through ticks thus a number of epidemiological factors/risk factors are involved. Ovine Babesiosis is one such disease posing problems in Pakistan which is an agricultural country and livestock plays an important source of income for farmers. The economic losses in small ruminant production are significant in the tropical and subtropical regions of the world. Carrier sheep infected with Babesiosis are challenge to current diagnostic methods and are difficult to detect because of the low number of parasites in circulation. However diagnosis of carrier animals in herd is important for preventing outbreaks by transmission through vector ticks to healthy animals and for obtaining epidemiological data of disease. The work done on Babesia ovis is negligible. For this purpose a study was conducted at two farms to measure the prevalence and optimization of PCR for Babesia ovis. Blood was collected into an anticoagulant containing vacutainer. First thin smears were formed and stained with Giemsa stain for microscopic examination of Babesia ovis. For DNA extraction Puregene DNA purification system, Gentra, was used. Extracted DNA was amplified in a thermolyser using B. ovis primers and then analyzed using electrophoresis on 1% agarose gel. Microscopic examination demonstrated a prevalence of 16 % while PCR results revealed prevalence of 29% for B. ovis. Results displayed that the efficacy of PCR is more sensitive than Light Microscopy. Data on infection rate between male and female and between different age groups was statistically non-significant. Herd wise prevalence was 36% and 22% in Livestock Production Research Institute, Bahadarnagar, Okara and Small Ruminant Training and Research Center,Ravi Campus, Pattoki respectively. Common sites of attachment for the ticks were under the tail, perineal region and underneath ears.The data was analyzed by using SPSS (Statistical Package for Social Sciences).
650 _aDepartment of Epidemiology & Public Health
_94991
700 _aDr. Muhammad Hassan Mushtaq
_95047
942 _cTH
999 _c3236
_d3236