000			02285nam a2200193Ia 4500
005			20151006134116.0
008			150525s2013 xx 000 0 und d
041			_aeng
082			_a1571,T
100			_aHasan Javed _98248
110			_cMs. Shagufta Saeed _98180
245			_aBioconversion Of Industrial Wastes To 6-Aminopencillanic Acid With Escherichia Coli.
260			_c2013
502			_a6-aminopenicillanic acid is ?-lactam nucleus produced by penicillin acylaseupon hydrolysis of penicillin. 6-APA is main component of semi-synthetic penicillins. Penicillin acylase is most valuable enzyme and is produced by many microbes such as Escherichia coli. Different media and method were used for the isolation, identification an characterization of E. coli. Total 30 strains of E. coli were isolated from fecal matter of equine species and tested for the penicillin acylase activity. About 13 isolates gave the enzyme activity. For the production of cell mass, different low cost media was used to cut down the price of production. Corn steep liquor, molasses, milk whey and wheat bran was tested for the growth of E. coli. These industrial wastes can minimize the production cost of 6-APA which has a high demand for the production of semi-synthetic penicillins. Corn steep liquor showed better growth of E. coli and can be used as the cheap source of carbon and nitrogen.Phenylacetic acid was also used in the growth medium and it was used as the inducer for enzyme. Without phenylacetic acid in medium, enzyme production decreases. Corn steep liquor is the best sources for production of cells which is 0.520 mg mL-1 Molasses also better for fermentation and highest value is 0.336 mg mL-1. Milk whey media needs further studies for the better production of cells with using different concentrations.it gave best production 0.112 mg mL-1 Wheat bran is not proper source for cell production and does no showed E. Coli growth. All the strains showed growth in corn steep liquor, milk whey and molasses but not in wheat bran. Among all the strains horse sample (Ho-9) showed better cell production in all the media used.
650			_aInstitute of Biochemistry & Biotechnology _95022
700			_aDr. Abu Saeed Hashmi _95240
700			_aDr. Muhammad _94698
942			_cTH
999			_c3272 _d3272