000			02170nam a2200229 a 4500
020			_a1588295494 (hardcover)
020			_a9781588295491 (hardcover)
082			_a572.84 Frank 19013 2nd 2006 Microbiology
100			_aPellestor, Franck. _913728
245	1	0	_aPRINS and In Situ PCR Protocols
250			_a2nd ed.
260			_aUSA: _bHumana Press; _c2006.
300			_a244 p. ;
490	1		_aMethods in Molecular Biology
520			_aThe in situ hybridization and PCR technologies are now well-established molecular techniques for studying chromosomal aneuploidy and rearran- ments, gene localization and expression, and genomic organization. Over the last decade, we have seen increasing applications in these fields. By combining the high sensitivity of the PCR reaction and the cytological localization of target sequences, both PRINS and in situ PCR techniques have provided highly powerful complements to FISH for in situ cellular and molecular investigations. Both these approaches have several advantages in terms of sensitivity and specificity, owing to the use of primers and to the fast kinetics of annealing and elongation reactions in situ. In the first edition of PRINS and In Situ PCR Protocols edited by John R. Gosden, experts in the field presented in detail a variety of applications of PRINS and in situ PCR techniques, in a wide range of clinical conditions. Since the publication of this successful reference book, there have been s- nificant improvements in in situ detection techniques. This completely revised and updated second edition presents a compreh- sive selection of new procedures developed in the field of PRINS and in situ PCR technologies. The book has two sections. Part I, Basic Methodology, contains chapters that provide useful protocols for many variations of PRINS and in situ PCR, including a new fast multicolor PRINS method, and protocols for PRINS detection of unique sequences in situ.
650			_aPolymerase chain reaction (PCR) _913727
650			_aDiagnosis of Infection _913277
650			_aDNA _92634
650			_aClinical Microbiology _913276
650			_aIn Situ Hybridization _913729
942			_cBK
999			_c5459 _d5459