000			01461nam a22002057a 4500
005			20151020131820.0
008			151020b2015 xxu||||| |||| 00| 0 eng d
041			_aeng
082			_a2315-T
100			_aArslan Sardar (2013-VA-282) _917200
110			_cDr. Imran Altaf _917199
245			_aGenetic Diversity Among Different Isolates Of Pasteurella Multocida From Poultry
260			_c2015
300			_a67p.;
502			_aFowl cholera is an acute bacterial disease of broiler breeders and layer breeders caused by Pasteurella multocida. In the present study, 10 isolates from different areas of Punjab were purified. These samples were confirmed by API Kit. Different molecular techniques like PCR and RFLP were used to investigate variation at the molecular level among 10 isolates collected from different areas of Punjab. Different mutations were observed among 10 field isolates at different mutation sites by sequencing. Phylogentic tree was also made using MEGA6 software that supported the sequencing results. ‘Msp1’ endonuclease cleaved bacterial whole genome at different cutting sites, all 10 isolates collected from different districts of Punjab cleaved into 3 to 5 fragments ranging from 600 to 10000 base pairs which showed the genetic variation among 10 isolates of P.mulocida.
650			_aDepartment of Microbiology _94609
700			_aProf. Dr. Aftab Ahmad Anjum _98817
700			_aDr. Sehrish Firyal _96653
942			_cTH
999			_c6376 _d6375